MD00G1105300.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004281
Physical Location & Seq
Forward (+)
22004253 .. 22004861
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1105300.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGCATTCTCATCTTCTAGCCATTTTGTCACATCTTTTCTCTTGGCACTTCTTATTAGTTGCTACATATTCTCTTCAACCTCAGCAGCAAGAGACCTTGCACAAAAAACCAACAATGAATTCATGAAAACATCTTGCAGTGCAACAACCTACCCAAAGCTTTGCCTTACTTCTCTTTCAAGACATTCAAGTGAAATCCAAACAAGCCCTAAACTTATGGCCAGCACAGCCCTCACCGTGACCCTTGCCTCCGCCAAATCAACCTCTACCATGATGTCAAAGCTCTCCCAAAGTCATGGCATGAAGCCTAGAGAAGTCGGAGCCATGAGGGACTGCATCGAAGAGTTAAGTGATTCGGTCGATGTCATTCAAAGGTCCATAGCTGAGATGGGCAACTTTACAAGTTCGGATTTTCAGCTCATGATAAGTGATGTGCAAACTTGGGTTAGTGCCGCTTTGACGGATGAGACAACATGCTCCGATGGGTTTGGTGGAAATGGGATGAACGGTAATTTGAAGACTGTTGTGAGGGGTAGAGTTGTGAATATTGCACAATTGACTAGCAATGCCTTGGCTCTGATCAACAGATACGCCTCAGTTCATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

203

Amino Acids

21.67

Weight (kDa)

7.66

Isoelectric Point (pI)

30.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 41 - 193 3.2e-39 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 252, 453
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 1 cut(s) 119
AgsI TTSAA 5 cut(s) 78, 180, 189, 371, 517
AjuI GAANNNNNNNTTGG 2 cut(s) 104, 136
AluBI AGCT 4 cut(s) 160, 283, 383, 418
AluI AGCT 4 cut(s) 160, 283, 383, 418
Alw26I GTCTC 2 cut(s) 87, 461
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 375
AsuHPI GGTGA 1 cut(s) 226
AvaII GGWCC 1 cut(s) 375
BalI TGGCCA 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 524
BbvCI CCTCAGC 1 cut(s) 82
BbvI GCAGC 1 cut(s) 98
BccI CCATC 2 cut(s) 382, 476
BclI TGATCA 1 cut(s) 579
BcoDI GTCTC 2 cut(s) 87, 461
BfaI CTAG 3 cut(s) 18, 309, 561
BisI GCNGC 2 cut(s) 87, 453
BlsI GCNGC 2 cut(s) 88, 454
Bme18I GGWCC 1 cut(s) 375
BmgT120I GGNCC 1 cut(s) 375
BmiI GGNNCC 1 cut(s) 322
BmsI GCATC 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 524
Bpu10I CCTNAGC 1 cut(s) 82
BsaI GGTCTC 1 cut(s) 87
BsaJI CCNNGG 1 cut(s) 570
Bse3DI GCAATG 1 cut(s) 571
BseDI CCNNGG 1 cut(s) 570
BseGI GGATG 2 cut(s) 469, 507
BseMI GCAATG 1 cut(s) 571
BseMII CTCAG 3 cut(s) 96, 375, 609
BseXI GCAGC 1 cut(s) 98
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 1 cut(s) 221
BsiEI CGRYCG 1 cut(s) 360
BslFI GGGAC 1 cut(s) 344
BsmAI GTCTC 2 cut(s) 87, 461
BsmFI GGGAC 1 cut(s) 344
BsmI GAATGC 1 cut(s) 5
BsnI GGCC 1 cut(s) 221
Bso31I GGTCTC 1 cut(s) 87
Bsp143I GATC 1 cut(s) 579
BspACI CCGC 2 cut(s) 252, 453
BspANI GGCC 1 cut(s) 221
BspCNI CTCAG 3 cut(s) 95, 376, 608
BspHI TCATGA 2 cut(s) 123, 420
BspLI GGNNCC 1 cut(s) 322
BspTNI GGTCTC 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 571
BssECI CCNNGG 1 cut(s) 570
BssMI GATC 1 cut(s) 579
BssT1I CCWWGG 1 cut(s) 570
Bst4CI ACNGT 3 cut(s) 238, 509, 523
Bst6I CTCTTC 2 cut(s) 79, 336
BstC8I GCNNGC 1 cut(s) 223
BstDEI CTNAG 3 cut(s) 82, 384, 595
BstF5I GGATG 2 cut(s) 469, 507
BstKTI GATC 1 cut(s) 582
BstMAI GTCTC 2 cut(s) 87, 461
BstMBI GATC 1 cut(s) 579
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 477
BstV1I GCAGC 1 cut(s) 98
BstV2I GAAGAC 1 cut(s) 524
BstXI CCANNNNNNTGG 1 cut(s) 296
BsuRI GGCC 1 cut(s) 221
BtsCI GGATG 2 cut(s) 469, 507
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 223
CciI TCATGA 2 cut(s) 123, 420
Cfr13I GGNCC 1 cut(s) 375
CviAII CATG 8 cut(s) 124, 271, 296, 301, 325, 421, 474, 602
DdeI CTNAG 3 cut(s) 82, 384, 595
DpnI GATC 1 cut(s) 581
DpnII GATC 1 cut(s) 579
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 2 cut(s) 79, 336
EarI CTCTTC 2 cut(s) 79, 336
EciI GGCGGA 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 570
Eco31I GGTCTC 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 375
EcoRI GAATTC 1 cut(s) 119
EcoT14I CCWWGG 1 cut(s) 570
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 8 cut(s) 127, 274, 299, 304, 328, 424, 477, 605
FaqI GGGAC 1 cut(s) 344
FatI CATG 8 cut(s) 123, 270, 295, 300, 324, 420, 473, 601
FbaI TGATCA 1 cut(s) 579
Fnu4HI GCNGC 2 cut(s) 87, 453
FokI GGATG 2 cut(s) 476, 514
Fsp4HI GCNGC 2 cut(s) 87, 453
FspBI CTAG 3 cut(s) 18, 309, 561
GluI GCNGC 2 cut(s) 87, 453
HaeIII GGCC 1 cut(s) 221
Hin1II CATG 8 cut(s) 127, 274, 299, 304, 328, 424, 477, 605
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 1 cut(s) 353
HphI GGTGA 1 cut(s) 226
Hpy188I TCNGA 4 cut(s) 320, 409, 481, 579
Hpy188III TCNNGA 3 cut(s) 124, 180, 421
HpyCH4III ACNGT 3 cut(s) 238, 509, 523
HpyCH4V TGCA 6 cut(s) 101, 138, 143, 336, 436, 551
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 3 cut(s) 82, 384, 595
Hsp92II CATG 8 cut(s) 127, 274, 299, 304, 328, 424, 477, 605
Ksp22I TGATCA 1 cut(s) 579
Kzo9I GATC 1 cut(s) 579
LmnI GCTCC 2 cut(s) 320, 482
LpnPI CCDG 1 cut(s) 235
Lsp1109I GCAGC 1 cut(s) 98
LweI GCATC 1 cut(s) 345
MaeI CTAG 3 cut(s) 18, 309, 561
MaeIII GTNAC 2 cut(s) 28, 238
MalI GATC 1 cut(s) 581
MboI GATC 1 cut(s) 579
MboII GAAGA 4 cut(s) 6, 66, 353, 529
MfeI CAATTG 1 cut(s) 554
MlsI TGGCCA 1 cut(s) 221
MluCI AATT 3 cut(s) 119, 511, 554
MluNI TGGCCA 1 cut(s) 221
MmeI TCCRAC 1 cut(s) 298
MnlI CCTC 7 cut(s) 91, 242, 259, 274, 321, 522, 604
Mox20I TGGCCA 1 cut(s) 221
MscI TGGCCA 1 cut(s) 221
MseI TTAA 2 cut(s) 347, 607
MslI CAYNNNNRTG 1 cut(s) 189
Msp20I TGGCCA 1 cut(s) 221
MunI CAATTG 1 cut(s) 554
Mva1269I GAATGC 1 cut(s) 5
MwoI GCNNNNNNNGC 1 cut(s) 227
NdeII GATC 1 cut(s) 579
NlaIII CATG 8 cut(s) 127, 274, 299, 304, 328, 424, 477, 605
NlaIV GGNNCC 1 cut(s) 322
NmuCI GTSAC 2 cut(s) 28, 238
NspI RCATGY 1 cut(s) 477
PagI TCATGA 2 cut(s) 123, 420
PctI GAATGC 1 cut(s) 5
PfeI GAWTC 1 cut(s) 353
PkrI GCNGC 2 cut(s) 88, 454
PspN4I GGNNCC 1 cut(s) 322
PspPI GGNCC 1 cut(s) 375
RseI CAYNNNNRTG 1 cut(s) 189
SaqAI TTAA 2 cut(s) 347, 607
SatI GCNGC 2 cut(s) 87, 453
Sau3AI GATC 1 cut(s) 579
Sau96I GGNCC 1 cut(s) 375
SetI ASST 9 cut(s) 83, 99, 152, 162, 266, 285, 377, 385, 420
SfaNI GCATC 1 cut(s) 345
SinI GGWCC 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 189
Sse9I AATT 3 cut(s) 119, 511, 554
SsiI CCGC 2 cut(s) 252, 453
SspI AATATT 1 cut(s) 547
SspMI CTAG 3 cut(s) 18, 309, 561
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 3 cut(s) 238, 509, 523
TaqI TCGA 2 cut(s) 339, 360
TaqII GACCGA 1 cut(s) 346
TasI AATT 3 cut(s) 119, 511, 554
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 1 cut(s) 353
Tru1I TTAA 2 cut(s) 347, 607
Tru9I TTAA 2 cut(s) 347, 607
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 2 cut(s) 28, 238
TseI GCWGC 1 cut(s) 86
Tsp45I GTSAC 2 cut(s) 28, 238
TspDTI ATGAA 6 cut(s) 112, 132, 140, 317, 518, 590
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 375
XapI RAATTY 1 cut(s) 119
XceI RCATGY 1 cut(s) 477
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XspI CTAG 3 cut(s) 18, 309, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.