AT4G30870

endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002805
Physical Location & Seq
Forward (+)
15028380 .. 15032645
4266 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30870.1

Sequence Viewer

Length: 1980 bp
ATGGATGACGAGAGACGGGTACTGTGCCCTGAGAACCGAGGTTTAGCGGCTTATGTGTTGCAGAAGAAGCAAGAATACGCAGAAAAGCCCAAAGGCTTGTCGGAAAATCTGGAGAGGACATTTGTTAAGGGGTATAGGAGTGTCTGCGACGCCAAGGATCCCATTAACACACTCAAAGACTTGTCGCAGATCAAGGGCTTTGGCAAGTGGATGGTTAAGCTCATGAAAGGATATTTTGATACTGCTGTTGAAAGTTCTGAGCAAGAAGATTTACCTGATAATCGTGCTGGGAAAAAGGCCAATGGAAAAAAACGGTACATTCCGCAAAGAAATTCAGTGGGATATGCATTACTAATAACGTTGCACAGAAGGACTACAAATGGGAAAGAATTCATGCGTAAACAAGAGCTTATTGATGCTGCTGACGCGAATGGGCTATCACACGCTCCTGTTGGGCCAGAGAAAGGGAAAGGGAAAGCAGGACTTGGACACTCTAAGAGGGAGTGGTATAGTGGATGGAGCTGCATGACCACTCTTATACAGAAGGGATTAGTGGTGAAATCTAGCAACCCAGCAAAGTACATGCTGACAGTTGAAGGTCGGGAAGTTGCAAATGAGTGTATCCTGCGATCTGGATTACCTGATTCAGTTGATATCTTATCTGTTGATGAAATGGATCCTACACCGCAAGCAAAGAAAACACCTAATCAAAATCCCACCTGTTCGTTTACCATGAGAGAAGAACTTCCATATGTCGATCCAAGATGTAGGGCACAATCAGCCATTCCAAGCGATATTCTTGAAAAGTTTACTCCGTTTGGTTATTCGAAGGAACAAGTCGTTGCTGCATTCAGAGAGGTTTCAGATGGATCTGGGGACAAAGATCCATCAACTCTCTGGCTCTCTGTAATGTGCCATCTTCGTCAAGCAGAGGTTTATAATTCCTGTCCGGACTCGAGAAATAGTAAAAAGGATTCATCAGGACCTTTTAAATCACAAATTCGTCAAGTTGATCTTGAGGGATCTCGTGCTAAGAAGTTCCGCTCTTGCAATGATGGATCCACCTTAAATCCATGTTCATCAGGTTCAAGTCATGCTGTGAAAGCCTGCTCATCTTCTTTGGCATCAGATGGAACCAAAGGAATAACAAATATTCCACGCCTCCCTCCTCTTCAATTTGGAGAAACGTTTGAGGAAGCATATGATGTGATCTTGATACTGGATGATCGAGAAAAGTTTGCCACCAAAGGATCACGATCCAGGAATATAGTTGAAAACATATGCTCTGAATTCAACATCAAGATTGAGGTTAGACGATTACCAGTCGGGGATTGTATATGGATTGCTCGGCATAAGTACCTTGAGACTGAATATGTTCTAGACTTTATTGCTGAGAGGAAGAACGTTGATGATATGCGCTCATCAATTAGAGATAATCGCTACAGAGACCAAAAACTTAGACTTCAGAGATCGGGATTCAAGAAGCTCATATACATTCTTGAAGGGGATCCAAACCATTCTGATGCAGCAGAAAGCATTAAAACAGCTTGTTTCACGACAGAGATTCTGGAGGGATTTGATGTGCTGAGGACACACGGGCTGGGTGAGACGCTAAGAAAATACGGCTACCTCACGAAATCAATATATCAATACTACAAGTTACGGGTGAATGATAATGACCAGAGCAAAGGCGCAGCCTCATGTCCTTCTTTTGATAGTTTTGTCAAAAGGTGTCAAGACCTTGATAAAATGACAATCAGCGATGTATTTGCCATTCAGCTAATGCAGGTCCCGCAGGTAACGGAGGAAATTGCCATAGCGGTTCTTGATATGTACCCAACGCTTTTGTCTCTTGCTTCTGCCTATTCTCATCTAGAAGCAGATGTCTCGGCGCAAGAAGAGATGTTGAGAAATAGAAGCAACAATGTAATATGTGCATCAGCTAGTAAGAATATATTCAAGTTAGTTTGGGGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

659

Amino Acids

74.06

Weight (kDa)

8.47

Isoelectric Point (pI)

48.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_MUS81 PF21136 108 - 205 6.5e-12 Crossover junction endonuclease MUS81-like, winged helix domain
ERCC4 PF02732 408 - 538 1e-24 ERCC4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 939
Acc36I ACCTGC 2 cut(s) 1777, 1786
AccBSI CCGCTC 1 cut(s) 1044
AccII CGCG 1 cut(s) 428
AccIII TCCGGA 1 cut(s) 949
AciI CCGC 6 cut(s) 47, 323, 686, 1042, 1793, 1820
AclI AACGTT 3 cut(s) 359, 1187, 1404
AcsI RAATTY 4 cut(s) 331, 389, 999, 1289
AcuI CTGAAG 1 cut(s) 1448
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 5 cut(s) 21, 317, 581, 1358, 1835
AfiI CCNNNNNNNGG 1 cut(s) 464
AjnI CCWGG 1 cut(s) 1259
AluBI AGCT 7 cut(s) 220, 409, 522, 1486, 1547, 1780, 1943
AluI AGCT 7 cut(s) 220, 409, 522, 1486, 1547, 1780, 1943
Alw26I GTCTC 6 cut(s) 7, 1358, 1440, 1601, 1854, 1891
Ama87I CYCGRG 1 cut(s) 955
Aor13HI TCCGGA 1 cut(s) 949
AoxI GGCC 2 cut(s) 297, 455
ApeKI GCWGC 5 cut(s) 419, 522, 845, 1526, 1694
ApoI RAATTY 4 cut(s) 331, 389, 999, 1289
Asp700I GAANNNNTTC 4 cut(s) 389, 744, 1374, 1955
AspLEI GCGC 3 cut(s) 1419, 1694, 1894
AspS9I GGNCC 3 cut(s) 455, 983, 1789
AsuHPI GGTGA 3 cut(s) 568, 1616, 1678
AsuII TTCGAA 1 cut(s) 827
AvaI CYCGRG 1 cut(s) 955
AvaII GGWCC 2 cut(s) 983, 1789
BaeGI GKGCMC 2 cut(s) 29, 775
BaeI ACNNNNGTAYC 2 cut(s) 231, 264
BamHI GGATCC 4 cut(s) 157, 676, 1058, 1507
BarI GAAGNNNNNNTAC 4 cut(s) 59, 91, 1475, 1507
BauI CACGAG 1 cut(s) 1026
BbvCI CCTCAGC 1 cut(s) 1586
BbvI GCAGC 5 cut(s) 406, 509, 832, 1538, 1706
BccI CCATC 7 cut(s) 205, 510, 860, 895, 924, 1049, 1124
BceAI ACGGC 1 cut(s) 1639
BcgI CGANNNNNNTGC 4 cut(s) 1751, 1785, 1869, 1903
BciT130I CCWGG 1 cut(s) 1261
BciVI GTATCC 1 cut(s) 632
BcoDI GTCTC 6 cut(s) 7, 1358, 1440, 1601, 1854, 1891
BfaI CTAG 4 cut(s) 564, 1379, 1874, 1944
BfmI CTRYAG 1 cut(s) 1441
BfuAI ACCTGC 2 cut(s) 1777, 1786
BfuI GTATCC 1 cut(s) 632
BisI GCNGC 6 cut(s) 48, 420, 523, 846, 1527, 1695
BlsI GCNGC 6 cut(s) 49, 421, 524, 847, 1528, 1696
Bme1390I CCNGG 1 cut(s) 1261
Bme18I GGWCC 2 cut(s) 983, 1789
BmeT110I CYCGRG 1 cut(s) 955
BmgT120I GGNCC 3 cut(s) 455, 983, 1789
BmiI GGNNCC 6 cut(s) 159, 678, 1060, 1135, 1509, 1791
BmrFI CCNGG 1 cut(s) 1261
BmsI GCATC 4 cut(s) 406, 1133, 1513, 1946
BpmI CTGGAG 2 cut(s) 131, 1589
Bpu10I CCTNAGC 1 cut(s) 1586
Bpu14I TTCGAA 1 cut(s) 827
BpuEI CTTGAG 2 cut(s) 1037, 1382
BsaBI GATNNNNATC 1 cut(s) 1255
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 1440
BsaJI CCNNGG 2 cut(s) 37, 153
BsaWI WCCGGW 1 cut(s) 949
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse1I ACTGG 2 cut(s) 1224, 1322
Bse3DI GCAATG 1 cut(s) 1057
Bse8I GATNNNNATC 1 cut(s) 1255
BseAI TCCGGA 1 cut(s) 949
BseBI CCWGG 1 cut(s) 1261
BseDI CCNNGG 2 cut(s) 37, 153
BseGI GGATG 4 cut(s) 10, 216, 521, 1228
BseJI GATNNNNATC 1 cut(s) 1255
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMI GCAATG 1 cut(s) 1057
BseMII CTCAG 4 cut(s) 21, 249, 1383, 1577
BseNI ACTGG 2 cut(s) 1224, 1322
BseRI GAGGAG 1 cut(s) 1158
BseSI GKGCMC 2 cut(s) 29, 775
BseXI GCAGC 5 cut(s) 406, 509, 832, 1538, 1706
BseYI CCCAGC 3 cut(s) 287, 571, 1600
Bsh1236I CGCG 1 cut(s) 428
BshFI GGCC 2 cut(s) 299, 457
BsiHKCI CYCGRG 1 cut(s) 955
BsiSI CCGG 1 cut(s) 950
BslFI GGGAC 2 cut(s) 890, 1775
BslI CCNNNNNNNGG 1 cut(s) 464
BsmAI GTCTC 6 cut(s) 7, 1358, 1440, 1601, 1854, 1891
BsmBI CGTCTC 2 cut(s) 7, 1601
BsmFI GGGAC 2 cut(s) 890, 1775
BsmI GAATGC 1 cut(s) 848
BsnI GGCC 2 cut(s) 299, 457
Bso31I GGTCTC 1 cut(s) 1440
BsoBI CYCGRG 1 cut(s) 955
Bsp119I TTCGAA 1 cut(s) 827
Bsp1286I GDGCHC 2 cut(s) 29, 775
Bsp13I TCCGGA 1 cut(s) 949
BspACI CCGC 6 cut(s) 47, 323, 686, 1042, 1793, 1820
BspANI GGCC 2 cut(s) 299, 457
BspCNI CTCAG 4 cut(s) 22, 250, 1384, 1578
BspEI TCCGGA 1 cut(s) 949
BspFNI CGCG 1 cut(s) 428
BspHI TCATGA 1 cut(s) 222
BspLI GGNNCC 6 cut(s) 159, 678, 1060, 1135, 1509, 1791
BspMI ACCTGC 2 cut(s) 1777, 1786
BspT104I TTCGAA 1 cut(s) 827
BspTNI GGTCTC 1 cut(s) 1440
BsrBI CCGCTC 1 cut(s) 1044
BsrDI GCAATG 1 cut(s) 1057
BsrI ACTGG 2 cut(s) 1224, 1322
BssECI CCNNGG 2 cut(s) 37, 153
BssNI GRCGYC 1 cut(s) 150
BssSI CACGAG 1 cut(s) 1026
BssT1I CCWWGG 1 cut(s) 153
Bst2BI CACGAG 1 cut(s) 1026
Bst2UI CCWGG 1 cut(s) 1261
Bst4CI ACNGT 3 cut(s) 24, 315, 592
Bst6I CTCTTC 2 cut(s) 1176, 1893
BstACI GRCGYC 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 827
BstC8I GCNNGC 2 cut(s) 690, 1108
BstDEI CTNAG 8 cut(s) 30, 258, 495, 1032, 1392, 1457, 1586, 1613
BstF5I GGATG 4 cut(s) 10, 216, 521, 1228
BstFNI CGCG 1 cut(s) 428
BstHHI GCGC 3 cut(s) 1419, 1694, 1894
BstMAI GTCTC 6 cut(s) 7, 1358, 1440, 1601, 1854, 1891
BstMWI GCNNNNNNNGC 5 cut(s) 67, 425, 779, 1103, 1792
BstNI CCWGG 1 cut(s) 1261
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 1 cut(s) 1259
BstSFI CTRYAG 1 cut(s) 1441
BstSLI GKGCMC 2 cut(s) 29, 775
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 5 cut(s) 406, 509, 832, 1538, 1706
BstX2I RGATCY 7 cut(s) 157, 676, 869, 883, 1022, 1058, 1507
BstYI RGATCY 7 cut(s) 157, 676, 869, 883, 1022, 1058, 1507
BsuI GTATCC 1 cut(s) 632
BsuRI GGCC 2 cut(s) 299, 457
BtgZI GCGATG 1 cut(s) 1776
BtsCI GGATG 4 cut(s) 10, 216, 521, 1228
BtsIMutI CAGTG 1 cut(s) 342
BveI ACCTGC 2 cut(s) 1777, 1786
Cac8I GCNNGC 2 cut(s) 690, 1108
CciI TCATGA 1 cut(s) 222
CfoI GCGC 3 cut(s) 1419, 1694, 1894
Cfr13I GGNCC 3 cut(s) 455, 983, 1789
CseI GACGC 3 cut(s) 158, 434, 1618
Csp6I GTAC 5 cut(s) 20, 316, 580, 1357, 1834
CviAII CATG 8 cut(s) 223, 394, 526, 583, 733, 1074, 1094, 1701
CviQI GTAC 5 cut(s) 20, 316, 580, 1357, 1834
DdeI CTNAG 8 cut(s) 30, 258, 495, 1032, 1392, 1457, 1586, 1613
DraI TTTAAA 1 cut(s) 991
Eam1104I CTCTTC 2 cut(s) 1176, 1893
EarI CTCTTC 2 cut(s) 1176, 1893
Eco130I CCWWGG 1 cut(s) 153
Eco31I GGTCTC 1 cut(s) 1440
Eco32I GATATC 1 cut(s) 655
Eco47I GGWCC 2 cut(s) 983, 1789
Eco57I CTGAAG 1 cut(s) 1448
Eco88I CYCGRG 1 cut(s) 955
EcoO109I RGGNCCY 2 cut(s) 983, 1789
EcoRI GAATTC 2 cut(s) 389, 1289
EcoRII CCWGG 1 cut(s) 1259
EcoRV GATATC 1 cut(s) 655
EcoT14I CCWWGG 1 cut(s) 153
EcoT22I ATGCAT 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 153
Esp3I CGTCTC 2 cut(s) 7, 1601
FaeI CATG 8 cut(s) 226, 397, 529, 586, 736, 1077, 1097, 1704
FalI AAGNNNNNCTT 4 cut(s) 468, 500, 999, 1031
FaqI GGGAC 2 cut(s) 890, 1775
FatI CATG 8 cut(s) 222, 393, 525, 582, 732, 1073, 1093, 1700
FauI CCCGC 1 cut(s) 1800
FauNDI CATATG 3 cut(s) 751, 1201, 1280
Fnu4HI GCNGC 6 cut(s) 48, 420, 523, 846, 1527, 1695
FokI GGATG 4 cut(s) 17, 223, 528, 1235
Fsp4HI GCNGC 6 cut(s) 48, 420, 523, 846, 1527, 1695
FspBI CTAG 4 cut(s) 564, 1379, 1874, 1944
GlaI GCGC 3 cut(s) 1418, 1693, 1893
GluI GCNGC 6 cut(s) 48, 420, 523, 846, 1527, 1695
GsaI CCCAGC 3 cut(s) 291, 575, 1604
GsuI CTGGAG 2 cut(s) 131, 1589
HaeIII GGCC 2 cut(s) 299, 457
HapII CCGG 1 cut(s) 950
HgaI GACGC 3 cut(s) 158, 434, 1618
HhaI GCGC 3 cut(s) 1419, 1694, 1894
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 8 cut(s) 226, 397, 529, 586, 736, 1077, 1097, 1704
Hin6I GCGC 3 cut(s) 1417, 1692, 1892
HinP1I GCGC 3 cut(s) 1417, 1692, 1892
HinfI GANTC 5 cut(s) 644, 953, 974, 1476, 1564
HpaII CCGG 1 cut(s) 950
HphI GGTGA 3 cut(s) 568, 1616, 1678
Hpy166II GTNNAC 3 cut(s) 401, 729, 810
Hpy188I TCNGA 8 cut(s) 103, 259, 854, 865, 1129, 1288, 1467, 1522
Hpy8I GTNNAC 3 cut(s) 401, 729, 810
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 6 cut(s) 363, 538, 590, 823, 1496, 1716
HpyCH4III ACNGT 3 cut(s) 24, 315, 592
HpyCH4IV ACGT 3 cut(s) 359, 1187, 1404
HpyF10VI GCNNNNNNNGC 5 cut(s) 67, 425, 779, 1103, 1792
HpyF3I CTNAG 8 cut(s) 30, 258, 495, 1032, 1392, 1457, 1586, 1613
HpySE526I ACGT 3 cut(s) 359, 1187, 1404
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 8 cut(s) 226, 397, 529, 586, 736, 1077, 1097, 1704
HspAI GCGC 3 cut(s) 1417, 1692, 1892
Kpn2I TCCGGA 1 cut(s) 949
LmnI GCTCC 2 cut(s) 451, 519
Lsp1109I GCAGC 5 cut(s) 406, 509, 832, 1538, 1706
LweI GCATC 4 cut(s) 406, 1133, 1513, 1946
MaeI CTAG 4 cut(s) 564, 1379, 1874, 1944
MaeII ACGT 3 cut(s) 359, 1187, 1404
MaeIII GTNAC 2 cut(s) 1659, 1798
MbiI CCGCTC 1 cut(s) 1044
MboII GAAGA 8 cut(s) 76, 278, 752, 911, 1107, 1163, 1411, 1910
MflI RGATCY 7 cut(s) 157, 676, 869, 883, 1022, 1058, 1507
MhlI GDGCHC 2 cut(s) 29, 775
MluCI AATT 8 cut(s) 331, 389, 940, 999, 1175, 1289, 1425, 1809
MlyI GAGTC 1 cut(s) 947
MmeI TCCRAC 1 cut(s) 81
Mph1103I ATGCAT 1 cut(s) 349
MroI TCCGGA 1 cut(s) 949
MroXI GAANNNNTTC 4 cut(s) 389, 744, 1374, 1955
MseI TTAA 6 cut(s) 126, 165, 216, 990, 1067, 1539
MslI CAYNNNNRTG 1 cut(s) 1521
MspI CCGG 1 cut(s) 950
MspR9I CCNGG 1 cut(s) 1261
Mva1269I GAATGC 1 cut(s) 848
MvaI CCWGG 1 cut(s) 1261
MvnI CGCG 1 cut(s) 428
MwoI GCNNNNNNNGC 5 cut(s) 67, 425, 779, 1103, 1792
NdeI CATATG 3 cut(s) 751, 1201, 1280
NlaIII CATG 8 cut(s) 226, 397, 529, 586, 736, 1077, 1097, 1704
NlaIV GGNNCC 6 cut(s) 159, 678, 1060, 1135, 1509, 1791
NmeAIII GCCGAG 2 cut(s) 1327, 1868
NsiI ATGCAT 1 cut(s) 349
NspI RCATGY 1 cut(s) 586
NspV TTCGAA 1 cut(s) 827
PaeR7I CTCGAG 1 cut(s) 955
PagI TCATGA 1 cut(s) 222
PctI GAATGC 1 cut(s) 848
PdmI GAANNNNTTC 4 cut(s) 389, 744, 1374, 1955
PfeI GAWTC 4 cut(s) 644, 974, 1476, 1564
PfoI TCCNGGA 1 cut(s) 1259
PkrI GCNGC 6 cut(s) 49, 421, 524, 847, 1528, 1696
PleI GAGTC 1 cut(s) 947
PpsI GAGTC 1 cut(s) 947
PpuMI RGGWCCY 2 cut(s) 983, 1789
PsiI TTATAA 1 cut(s) 939
Psp1406I AACGTT 3 cut(s) 359, 1187, 1404
Psp5II RGGWCCY 2 cut(s) 983, 1789
Psp6I CCWGG 1 cut(s) 1259
PspFI CCCAGC 3 cut(s) 287, 571, 1600
PspGI CCWGG 1 cut(s) 1259
PspN4I GGNNCC 6 cut(s) 159, 678, 1060, 1135, 1509, 1791
PspPI GGNCC 3 cut(s) 455, 983, 1789
PspPPI RGGWCCY 2 cut(s) 983, 1789
PsuI RGATCY 7 cut(s) 157, 676, 869, 883, 1022, 1058, 1507
RsaI GTAC 5 cut(s) 21, 317, 581, 1358, 1835
RsaNI GTAC 5 cut(s) 20, 316, 580, 1357, 1834
RseI CAYNNNNRTG 1 cut(s) 1521
SaqAI TTAA 6 cut(s) 126, 165, 216, 990, 1067, 1539
SatI GCNGC 6 cut(s) 48, 420, 523, 846, 1527, 1695
Sau96I GGNCC 3 cut(s) 455, 983, 1789
SchI GAGTC 1 cut(s) 947
ScrFI CCNGG 1 cut(s) 1261
SduI GDGCHC 2 cut(s) 29, 775
SfaNI GCATC 4 cut(s) 406, 1133, 1513, 1946
SfcI CTRYAG 1 cut(s) 1441
Sfr274I CTCGAG 1 cut(s) 955
SfuI TTCGAA 1 cut(s) 827
SinI GGWCC 2 cut(s) 983, 1789
SlaI CTCGAG 1 cut(s) 955
SmiMI CAYNNNNRTG 1 cut(s) 1521
SmlI CTYRAG 3 cut(s) 955, 1016, 1361
SmoI CTYRAG 3 cut(s) 955, 1016, 1361
Sse9I AATT 8 cut(s) 331, 389, 940, 999, 1175, 1289, 1425, 1809
SsiI CCGC 6 cut(s) 47, 323, 686, 1042, 1793, 1820
SspI AATATT 1 cut(s) 1153
SspMI CTAG 4 cut(s) 564, 1379, 1874, 1944
StyD4I CCNGG 1 cut(s) 1259
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 3 cut(s) 24, 315, 592
TaiI ACGT 3 cut(s) 362, 1190, 1407
TaqI TCGA 4 cut(s) 756, 827, 956, 1228
TasI AATT 8 cut(s) 331, 389, 940, 999, 1175, 1289, 1425, 1809
TatI WGTACW 1 cut(s) 579
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 4 cut(s) 644, 974, 1476, 1564
Tru1I TTAA 6 cut(s) 126, 165, 216, 990, 1067, 1539
Tru9I TTAA 6 cut(s) 126, 165, 216, 990, 1067, 1539
TscAI CASTG 1 cut(s) 342
TseI GCWGC 5 cut(s) 419, 522, 845, 1526, 1694
TspDTI ATGAA 5 cut(s) 239, 382, 684, 966, 1068
TspGWI ACGGA 2 cut(s) 804, 1817
TspRI CASTG 1 cut(s) 342
VpaK11BI GGWCC 2 cut(s) 983, 1789
XapI RAATTY 4 cut(s) 331, 389, 999, 1289
XbaI TCTAGA 2 cut(s) 1378, 1873
XceI RCATGY 1 cut(s) 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 894
XhoI CTCGAG 1 cut(s) 955
XmnI GAANNNNTTC 4 cut(s) 389, 744, 1374, 1955
XspI CTAG 4 cut(s) 564, 1379, 1874, 1944
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.