Prupe.2G032400_v2.0.a1

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0002805
Physical Location & Seq
Forward (+)
3493512 .. 3498481
4970 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G032400.1

Sequence Viewer

Length: 906 bp
ATGGAGAAGAGTGAACAGAGAAGGCAGAAGGCAGAAGGCAGAAGGCAGGAGGTGAGAGGGACCAGAGGGTTACGTCATGGAACTTGGATTGGTGGTAAAAGAAACAAAGGAAGCAGGCGCTATCTTCCACAAAAGAATTATGTGGCATATGCATTATTGATCACACTTTACAGGGGAACTGAAAATGTGAACGAATTTATGCCAAGTGGACTTTCTCATATGCCAATTATGTCAGAGAAGGGAAAGGGAAAACTTGGACACTTTGCGAGTTCTAAAAGGAATGAAATGTTTAGATTGTTTAGGCCAGATCCACTTGCCCTATTCTTGGTAACCATCTATGTTACTTTTATTTTCCCAGTCTTTGTAGACAATGTTGTTTGGTGTGGGCTAAAGAAACTGATGTATCTTGTGGAAAGTGATCCAAATACTTCTGAAGCAGCAGAAAGCATGGAAACAGACTGTTTCACAACTGAGATGTTGGAGGGATTTGACGTTCAAAGAACCATTGGCTTGACTGATACATTGAAGACGTATGCGTATCTTACTCAAGCAATTACTCAATATTACAATTCAGAATTCTCTGAGGAACAATATATACGTGCTGAGGAACCTTCTGCATGTCCTTTTAATTGTTACAATTCAGAATTCTCTTTTGAAACAGATAGTGAGAAAATGGTGAACAAACTTTACACACTTCTTCAATCATGTATGCAAGTCTCCCAGTTAACTGAGGAAGTTGTCATAGTTGTTCTGGATTTGTACCTAACGCTTTTATCTCTTACCCATGCCTATTCTCTACTTGAGGGCGATGTCTGTGCACAAGAAGAGATGCTTAGAACCCAGAGTAACAATGCTGTCAATGCGGGTGCTAGTAAGAATATATTTCACTTGGTTTGGGGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

302

Amino Acids

34.48

Weight (kDa)

5.85

Isoelectric Point (pI)

53.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 366
AciI CCGC 1 cut(s) 863
AclWI GGATC 2 cut(s) 302, 413
AcsI RAATTY 3 cut(s) 194, 575, 644
AcuI CTGAAG 1 cut(s) 453
AfaI GTAC 1 cut(s) 761
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 4 cut(s) 497, 526, 656, 701
AjuI GAANNNNNNNTTGG 2 cut(s) 196, 228
AloI GAACNNNNNNTCC 2 cut(s) 477, 509
Alw21I GWGCWC 1 cut(s) 820
Alw26I GTCTC 1 cut(s) 721
Alw44I GTGCAC 1 cut(s) 816
AlwI GGATC 2 cut(s) 302, 413
AoxI GGCC 1 cut(s) 302
ApaLI GTGCAC 1 cut(s) 816
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 3 cut(s) 194, 575, 644
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 60
AsuHPI GGTGA 2 cut(s) 64, 688
AvaII GGWCC 1 cut(s) 60
BaeGI GKGCMC 1 cut(s) 820
BaeI ACNNNNGTAYC 2 cut(s) 386, 419
BbsI GAAGAC 1 cut(s) 533
Bbv12I GWGCWC 1 cut(s) 820
BbvCI CCTCAGC 1 cut(s) 603
BbvI GCAGC 1 cut(s) 449
BccI CCATC 1 cut(s) 341
BcgI CGANNNNNNTGC 2 cut(s) 797, 831
BclI TGATCA 1 cut(s) 159
BcoDI GTCTC 1 cut(s) 721
BfaI CTAG 1 cut(s) 870
BfoI RGCGCY 1 cut(s) 121
BisI GCNGC 1 cut(s) 438
BlsI GCNGC 1 cut(s) 439
Bme18I GGWCC 1 cut(s) 60
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 2 cut(s) 61, 609
BmrI ACTGGG 2 cut(s) 350, 715
BmsI GCATC 1 cut(s) 819
BmuI ACTGGG 2 cut(s) 350, 715
BpiI GAAGAC 1 cut(s) 533
Bpu10I CCTNAGC 1 cut(s) 603
BpuEI CTTGAG 2 cut(s) 531, 821
BsaAI YACGTR 1 cut(s) 599
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 2 cut(s) 356, 721
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMII CTCAG 4 cut(s) 462, 573, 594, 720
BseNI ACTGG 2 cut(s) 356, 721
BseSI GKGCMC 1 cut(s) 820
BseXI GCAGC 1 cut(s) 449
BshFI GGCC 1 cut(s) 304
BsiHKAI GWGCWC 1 cut(s) 820
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 1 cut(s) 325
BsmAI GTCTC 1 cut(s) 721
BsmFI GGGAC 1 cut(s) 73
BsnI GGCC 1 cut(s) 304
Bsp1286I GDGCHC 1 cut(s) 820
Bsp143I GATC 3 cut(s) 159, 307, 418
BspACI CCGC 1 cut(s) 863
BspANI GGCC 1 cut(s) 304
BspCNI CTCAG 4 cut(s) 463, 574, 595, 721
BspLI GGNNCC 2 cut(s) 61, 609
BspPI GGATC 2 cut(s) 302, 413
BsrI ACTGG 2 cut(s) 356, 721
BssMI GATC 3 cut(s) 159, 307, 418
Bst4CI ACNGT 1 cut(s) 461
Bst6I CTCTTC 2 cut(s) 2, 819
BstBAI YACGTR 1 cut(s) 599
BstC8I GCNNGC 1 cut(s) 116
BstDEI CTNAG 5 cut(s) 471, 582, 603, 729, 833
BstEII GGTNACC 1 cut(s) 328
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 3 cut(s) 162, 310, 421
BstMAI GTCTC 1 cut(s) 721
BstMBI GATC 3 cut(s) 159, 307, 418
BstMWI GCNNNNNNNGC 1 cut(s) 860
BstNSI RCATGY 1 cut(s) 621
BstPI GGTNACC 1 cut(s) 328
BstSLI GKGCMC 1 cut(s) 820
BstV1I GCAGC 1 cut(s) 449
BstV2I GAAGAC 1 cut(s) 533
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 1 cut(s) 304
BtgZI GCGATG 1 cut(s) 822
Cac8I GCNNGC 1 cut(s) 116
CfoI GCGC 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 60
Csp6I GTAC 1 cut(s) 760
CviAII CATG 5 cut(s) 77, 448, 618, 705, 785
CviJI RGCY 3 cut(s) 304, 388, 510
CviKI_1 RGCY 3 cut(s) 304, 388, 510
CviQI GTAC 1 cut(s) 760
DdeI CTNAG 5 cut(s) 471, 582, 603, 729, 833
DpnI GATC 3 cut(s) 161, 309, 420
DpnII GATC 3 cut(s) 159, 307, 418
Eam1104I CTCTTC 2 cut(s) 2, 819
EarI CTCTTC 2 cut(s) 2, 819
Eco47I GGWCC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 453
Eco91I GGTNACC 1 cut(s) 328
EcoO65I GGTNACC 1 cut(s) 328
EcoRI GAATTC 2 cut(s) 575, 644
EcoT22I ATGCAT 1 cut(s) 154
FaeI CATG 5 cut(s) 80, 451, 621, 708, 788
FalI AAGNNNNNCTT 2 cut(s) 816, 848
FaqI GGGAC 1 cut(s) 73
FatI CATG 5 cut(s) 76, 447, 617, 704, 784
FauI CCCGC 1 cut(s) 856
FauNDI CATATG 2 cut(s) 148, 219
FbaI TGATCA 1 cut(s) 159
FblI GTMKAC 1 cut(s) 366
Fnu4HI GCNGC 1 cut(s) 438
Fsp4HI GCNGC 1 cut(s) 438
FspBI CTAG 1 cut(s) 870
GlaI GCGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 438
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 1 cut(s) 304
HhaI GCGC 1 cut(s) 120
Hin1II CATG 5 cut(s) 80, 451, 621, 708, 788
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HpaI GTTAAC 1 cut(s) 726
HphI GGTGA 2 cut(s) 64, 688
Hpy166II GTNNAC 7 cut(s) 14, 190, 209, 367, 679, 726, 818
Hpy188I TCNGA 5 cut(s) 235, 433, 574, 583, 643
Hpy188III TCNNGA 1 cut(s) 752
Hpy8I GTNNAC 7 cut(s) 14, 190, 209, 367, 679, 726, 818
HpyAV CCTTC 6 cut(s) 15, 22, 29, 36, 232, 621
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4IV ACGT 4 cut(s) 73, 492, 530, 598
HpyCH4V TGCA 4 cut(s) 152, 617, 712, 818
HpyF10VI GCNNNNNNNGC 1 cut(s) 860
HpyF3I CTNAG 5 cut(s) 471, 582, 603, 729, 833
HpySE526I ACGT 4 cut(s) 73, 492, 530, 598
Hsp92II CATG 5 cut(s) 80, 451, 621, 708, 788
HspAI GCGC 1 cut(s) 118
Ksp22I TGATCA 1 cut(s) 159
KspAI GTTAAC 1 cut(s) 726
Kzo9I GATC 3 cut(s) 159, 307, 418
LpnPI CCDG 9 cut(s) 32, 76, 100, 157, 318, 369, 734, 737, 854
Lsp1109I GCAGC 1 cut(s) 449
LweI GCATC 1 cut(s) 819
MaeI CTAG 1 cut(s) 870
MaeII ACGT 4 cut(s) 73, 492, 530, 598
MaeIII GTNAC 5 cut(s) 69, 328, 340, 632, 845
MalI GATC 3 cut(s) 161, 309, 420
MboI GATC 3 cut(s) 159, 307, 418
MboII GAAGA 5 cut(s) 19, 116, 538, 689, 836
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 820
MluCI AATT 9 cut(s) 136, 194, 225, 552, 568, 575, 628, 637, 644
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 8 cut(s) 43, 50, 59, 475, 577, 598, 724, 796
Mph1103I ATGCAT 1 cut(s) 154
MseI TTAA 2 cut(s) 627, 725
MwoI GCNNNNNNNGC 1 cut(s) 860
NdeI CATATG 2 cut(s) 148, 219
NdeII GATC 3 cut(s) 159, 307, 418
NlaIII CATG 5 cut(s) 80, 451, 621, 708, 788
NlaIV GGNNCC 2 cut(s) 61, 609
NsiI ATGCAT 1 cut(s) 154
NspI RCATGY 1 cut(s) 621
PkrI GCNGC 1 cut(s) 439
Ppu21I YACGTR 1 cut(s) 599
PspEI GGTNACC 1 cut(s) 328
PspN4I GGNNCC 2 cut(s) 61, 609
PspPI GGNCC 1 cut(s) 60
PsrI GAACNNNNNNTAC 4 cut(s) 579, 611, 671, 703
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 1 cut(s) 761
RsaNI GTAC 1 cut(s) 760
SaqAI TTAA 2 cut(s) 627, 725
SatI GCNGC 1 cut(s) 438
Sau3AI GATC 3 cut(s) 159, 307, 418
Sau96I GGNCC 1 cut(s) 60
SduI GDGCHC 1 cut(s) 820
SetI ASST 7 cut(s) 54, 76, 495, 533, 601, 613, 765
SfaNI GCATC 1 cut(s) 819
SinI GGWCC 1 cut(s) 60
SmlI CTYRAG 2 cut(s) 546, 800
SmoI CTYRAG 2 cut(s) 546, 800
Sse9I AATT 9 cut(s) 136, 194, 225, 552, 568, 575, 628, 637, 644
SsiI CCGC 1 cut(s) 863
SspI AATATT 1 cut(s) 563
SspMI CTAG 1 cut(s) 870
TaaI ACNGT 1 cut(s) 461
TaiI ACGT 4 cut(s) 76, 495, 533, 601
TasI AATT 9 cut(s) 136, 194, 225, 552, 568, 575, 628, 637, 644
Tru1I TTAA 2 cut(s) 627, 725
Tru9I TTAA 2 cut(s) 627, 725
TseI GCWGC 1 cut(s) 437
TspDTI ATGAA 1 cut(s) 297
VneI GTGCAC 1 cut(s) 816
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 3 cut(s) 194, 575, 644
XceI RCATGY 1 cut(s) 621
XmiI GTMKAC 1 cut(s) 366
XspI CTAG 1 cut(s) 870
Zsp2I ATGCAT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.