RLG00000022919

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002805
Physical Location & Seq
Reverse (-)
15152165 .. 15160511
8347 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022919

Sequence Viewer

Length: 1338 bp
ATGAGGGATCCACTGGCGTTCCGTACCTCGAGTGGATCAGCCAAGAGAACCAAATCAACCAAACGCTACGTTCCGCAAGAGAATTCCGTTTCATATGCTTTGTTGATCACGCTTTACAGGGGAACCGAAAATGGAGATGAATTTATGCGTAAACAGGAGCTTATTGATGCAGCTGAAGCAAGTGGACTTTCTCGTGTGCCGATTATGCCGGAGAAGGGGAAGAGAAAACCTACAACTCTCGGCAATTCTGCCAAGGAATGGTATAGTGGTTGGTCCTGCATGAAGACACTGATAACCAGAGGACTGGTTCTGAAATTAAGTTGTCCGGCAAAGTACATGCTTACTGAAGAAGGTCAGGAAGTTGCCCGTGAATGTCTCATGAGGTCTGGTTTGGCGGATCCTGTGGAAAAAGTTTCTAATGCCAAAGGGTTGTCTGTCGTGGATGTGAATGTATCAGAACAGGGCCTTTCTCATCCTGAGTCAGTGATAGATATGACATTGTTGTCCAGTAATTCAAGTAGTGAGGAGAACTTAATTGATATGCCATGGGAATGTCTTGAAAAGAGTTGTCCCGTGCCAAAGTCTAGTTCTCCGGGTGTCAAAGCAAGTTCAAAAGCTTTATCCATTCCACCTCTAAGCTTTGGGGAGAGATTTGAAGATGCTTATGAAGTAGTCAGACGGTTAACAGTTGGAGATGGGATTTGGATAGCTCGCCATAATTATCTTGAGACTGAATATGTACTTGATTTTATTGTTGAGAGGAAGAATGTTGATGATTTACGCTGTTCAATAAGAGACAACCGTTACAGGGATCAAAAATTGAGACTTTTGAGGTGCGGACTCAAAAAGCTGATATATCTTGTGGAAGGTGATCCCAATTCTTGTGCAGCAGCAGAAAGCATTAAAACAGCCTGCTTCACAACTGAAATTTTGGAGGGTTTTGATGTTCAGAGAACTGAAAACTTGACTGAAACCTTAAGGAAGTATGGTTATCTTACTGAAGCAATATTTAAATATTACAAAGAACTTCCTGAGGAACAGCGTAAGCATGCTGGAGTTTGTCTTAAATTTGATGAATTTAACAAAAGGAGTCAAGACCTGGATAAAATGACAGTCAGTGACGTATTTGCTGTTCAGCTCATGCAGGTCCCCCAGGTGACTGAGGAAGTTGCCATTGCTGTTCTGGATTTGTACCCAACGCCTTTTTCTCTTGCCCATGAATACTCTCTACTTGAGGGTGATGTCCGCACACAGGAGGAGATGCTTAGAACACAGAGTAACAATGTTGTAAACGCTGCTGCCAGTAAGAATATTTTTCGCTTTGTATGGAGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

446

Amino Acids

50.25

Weight (kDa)

5.87

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_MUS81 PF21136 25 - 123 2e-12 Crossover junction endonuclease MUS81-like, winged helix domain
ERCC4 PF02732 224 - 326 1.2e-20 ERCC4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 502
Acc36I ACCTGC 1 cut(s) 1135
AccB7I CCANNNNNTGG 1 cut(s) 258
AciI CCGC 4 cut(s) 74, 395, 837, 1246
AclWI GGATC 7 cut(s) 2, 15, 43, 392, 405, 819, 866
AcsI RAATTY 5 cut(s) 82, 140, 927, 1067, 1076
AcuI CTGAAG 3 cut(s) 195, 366, 1020
AfaI GTAC 4 cut(s) 25, 335, 741, 1193
AfiI CCNNNNNNNGG 4 cut(s) 215, 258, 808, 1252
AflII CTTAAG 1 cut(s) 976
AgsI TTSAA 5 cut(s) 516, 560, 612, 656, 789
AjnI CCWGG 2 cut(s) 1098, 1152
AjuI GAANNNNNNNTTGG 4 cut(s) 571, 603, 1189, 1221
AluBI AGCT 7 cut(s) 160, 173, 617, 639, 710, 850, 1138
AluI AGCT 7 cut(s) 160, 173, 617, 639, 710, 850, 1138
Alw26I GTCTC 4 cut(s) 380, 722, 789, 817
AlwI GGATC 7 cut(s) 2, 15, 43, 392, 405, 819, 866
Ama87I CYCGRG 1 cut(s) 28
AoxI GGCC 1 cut(s) 463
ApeKI GCWGC 5 cut(s) 170, 887, 890, 1295, 1298
ApoI RAATTY 5 cut(s) 82, 140, 927, 1067, 1076
AspS9I GGNCC 3 cut(s) 273, 463, 1147
AsuC2I CCSGG 1 cut(s) 594
AsuHPI GGTGA 3 cut(s) 881, 1168, 1250
AvaI CYCGRG 1 cut(s) 28
AvaII GGWCC 2 cut(s) 273, 1147
AxyI CCTNAGG 1 cut(s) 1032
BamHI GGATCC 2 cut(s) 7, 397
BauI CACGAG 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 290
BbvI GCAGC 5 cut(s) 182, 899, 902, 1282, 1285
BccI CCATC 1 cut(s) 689
BciT130I CCWGG 2 cut(s) 1100, 1154
BclI TGATCA 1 cut(s) 105
BcnI CCSGG 1 cut(s) 594
BcoDI GTCTC 4 cut(s) 380, 722, 789, 817
BfaI CTAG 1 cut(s) 585
BfrI CTTAAG 1 cut(s) 976
BfuAI ACCTGC 1 cut(s) 1135
BisI GCNGC 5 cut(s) 171, 888, 891, 1296, 1299
BlsI GCNGC 5 cut(s) 172, 889, 892, 1297, 1300
Bme1390I CCNGG 3 cut(s) 594, 1100, 1154
Bme18I GGWCC 2 cut(s) 273, 1147
BmeT110I CYCGRG 1 cut(s) 28
BmgT120I GGNCC 3 cut(s) 273, 463, 1147
BmiI GGNNCC 4 cut(s) 9, 124, 399, 1149
BmrFI CCNGG 3 cut(s) 594, 1100, 1154
BmsI GCATC 3 cut(s) 157, 649, 1251
BpiI GAAGAC 1 cut(s) 290
BpmI CTGGAG 1 cut(s) 1074
BpuEI CTTGAG 2 cut(s) 746, 1253
BpuMI CCSGG 1 cut(s) 594
BsaJI CCNNGG 3 cut(s) 252, 545, 1152
Bsc4I CCNNNNNNNGG 4 cut(s) 215, 258, 808, 1252
Bse1I ACTGG 4 cut(s) 18, 309, 507, 1302
Bse21I CCTNAGG 1 cut(s) 1032
Bse3DI GCAATG 1 cut(s) 1173
BseBI CCWGG 2 cut(s) 1100, 1154
BseDI CCNNGG 3 cut(s) 252, 545, 1152
BseGI GGATG 2 cut(s) 448, 472
BseLI CCNNNNNNNGG 4 cut(s) 215, 258, 808, 1252
BseMI GCAATG 1 cut(s) 1173
BseMII CTCAG 3 cut(s) 468, 1023, 1152
BseNI ACTGG 4 cut(s) 18, 309, 507, 1302
BseRI GAGGAG 2 cut(s) 539, 1271
BseXI GCAGC 5 cut(s) 182, 899, 902, 1282, 1285
BsgI GTGCAG 1 cut(s) 906
BshFI GGCC 1 cut(s) 465
BsiHKCI CYCGRG 1 cut(s) 28
BsiSI CCGG 3 cut(s) 209, 326, 593
BslFI GGGAC 2 cut(s) 555, 1133
BslI CCNNNNNNNGG 4 cut(s) 215, 258, 808, 1252
BsmAI GTCTC 4 cut(s) 380, 722, 789, 817
BsmFI GGGAC 2 cut(s) 555, 1133
BsnI GGCC 1 cut(s) 465
BsoBI CYCGRG 1 cut(s) 28
Bsp143I GATC 6 cut(s) 7, 35, 105, 397, 811, 871
Bsp19I CCATGG 1 cut(s) 545
BspACI CCGC 4 cut(s) 74, 395, 837, 1246
BspANI GGCC 1 cut(s) 465
BspCNI CTCAG 3 cut(s) 469, 1024, 1153
BspHI TCATGA 1 cut(s) 378
BspLI GGNNCC 4 cut(s) 9, 124, 399, 1149
BspMI ACCTGC 1 cut(s) 1135
BspPI GGATC 7 cut(s) 2, 15, 43, 392, 405, 819, 866
BspTI CTTAAG 1 cut(s) 976
BsrDI GCAATG 1 cut(s) 1173
BsrI ACTGG 4 cut(s) 18, 309, 507, 1302
BssECI CCNNGG 3 cut(s) 252, 545, 1152
BssMI GATC 6 cut(s) 7, 35, 105, 397, 811, 871
BssSI CACGAG 1 cut(s) 192
BssT1I CCWWGG 2 cut(s) 252, 545
Bst2BI CACGAG 1 cut(s) 192
Bst2UI CCWGG 2 cut(s) 1100, 1154
Bst4CI ACNGT 4 cut(s) 681, 688, 803, 1114
Bst6I CTCTTC 1 cut(s) 215
BstAFI CTTAAG 1 cut(s) 976
BstC8I GCNNGC 3 cut(s) 712, 913, 1050
BstDEI CTNAG 5 cut(s) 477, 635, 1032, 1161, 1265
BstDSI CCRYGG 1 cut(s) 545
BstF5I GGATG 2 cut(s) 448, 472
BstKTI GATC 6 cut(s) 10, 38, 108, 400, 814, 874
BstMAI GTCTC 4 cut(s) 380, 722, 789, 817
BstMBI GATC 6 cut(s) 7, 35, 105, 397, 811, 871
BstMWI GCNNNNNNNGC 2 cut(s) 176, 205
BstNI CCWGG 2 cut(s) 1100, 1154
BstNSI RCATGY 2 cut(s) 340, 1052
BstSCI CCNGG 3 cut(s) 592, 1098, 1152
BstV1I GCAGC 5 cut(s) 182, 899, 902, 1282, 1285
BstV2I GAAGAC 1 cut(s) 290
BstX2I RGATCY 2 cut(s) 7, 397
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 2 cut(s) 7, 397
Bsu36I CCTNAGG 1 cut(s) 1032
BsuRI GGCC 1 cut(s) 465
BtgI CCRYGG 1 cut(s) 545
BtsCI GGATG 2 cut(s) 448, 472
BtsIMutI CAGTG 4 cut(s) 11, 287, 489, 1123
BveI ACCTGC 1 cut(s) 1135
Cac8I GCNNGC 3 cut(s) 712, 913, 1050
CciI TCATGA 1 cut(s) 378
Cfr13I GGNCC 3 cut(s) 273, 463, 1147
Csp6I GTAC 4 cut(s) 24, 334, 740, 1192
CviAII CATG 7 cut(s) 280, 337, 379, 546, 1049, 1141, 1217
CviQI GTAC 4 cut(s) 24, 334, 740, 1192
DdeI CTNAG 5 cut(s) 477, 635, 1032, 1161, 1265
DpnI GATC 6 cut(s) 9, 37, 107, 399, 813, 873
DpnII GATC 6 cut(s) 7, 35, 105, 397, 811, 871
DraI TTTAAA 1 cut(s) 1012
DrdI GACNNNNNNGTC 1 cut(s) 502
DseDI GACNNNNNNGTC 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
EciI GGCGGA 1 cut(s) 410
Eco130I CCWWGG 2 cut(s) 252, 545
Eco47I GGWCC 2 cut(s) 273, 1147
Eco57I CTGAAG 3 cut(s) 195, 366, 1020
Eco81I CCTNAGG 1 cut(s) 1032
Eco88I CYCGRG 1 cut(s) 28
EcoO109I RGGNCCY 2 cut(s) 463, 1147
EcoRI GAATTC 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 1098, 1152
EcoT14I CCWWGG 2 cut(s) 252, 545
ErhI CCWWGG 2 cut(s) 252, 545
FaeI CATG 7 cut(s) 283, 340, 382, 549, 1052, 1144, 1220
FaqI GGGAC 2 cut(s) 555, 1133
FatI CATG 7 cut(s) 279, 336, 378, 545, 1048, 1140, 1216
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 1 cut(s) 105
Fnu4HI GCNGC 5 cut(s) 171, 888, 891, 1296, 1299
FokI GGATG 2 cut(s) 455, 459
Fsp4HI GCNGC 5 cut(s) 171, 888, 891, 1296, 1299
FspBI CTAG 1 cut(s) 585
GluI GCNGC 5 cut(s) 171, 888, 891, 1296, 1299
GsuI CTGGAG 1 cut(s) 1074
HaeIII GGCC 1 cut(s) 465
HapII CCGG 3 cut(s) 209, 326, 593
Hin1II CATG 7 cut(s) 283, 340, 382, 549, 1052, 1144, 1220
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HindIII AAGCTT 2 cut(s) 615, 637
HinfI GANTC 3 cut(s) 479, 840, 1090
HpaI GTTAAC 1 cut(s) 684
HpaII CCGG 3 cut(s) 209, 326, 593
HphI GGTGA 3 cut(s) 881, 1168, 1250
Hpy166II GTNNAC 4 cut(s) 152, 185, 684, 1291
Hpy188I TCNGA 4 cut(s) 312, 457, 677, 951
Hpy188III TCNNGA 8 cut(s) 356, 379, 476, 557, 725, 1031, 1094, 1184
Hpy8I GTNNAC 4 cut(s) 152, 185, 684, 1291
HpyAV CCTTC 3 cut(s) 208, 344, 860
HpyCH4III ACNGT 4 cut(s) 681, 688, 803, 1114
HpyCH4IV ACGT 2 cut(s) 69, 1122
HpyCH4V TGCA 4 cut(s) 170, 279, 887, 1144
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 205
HpyF3I CTNAG 5 cut(s) 477, 635, 1032, 1161, 1265
HpySE526I ACGT 2 cut(s) 69, 1122
Hsp92II CATG 7 cut(s) 283, 340, 382, 549, 1052, 1144, 1220
Ksp22I TGATCA 1 cut(s) 105
KspAI GTTAAC 1 cut(s) 684
Kzo9I GATC 6 cut(s) 7, 35, 105, 397, 811, 871
LmnI GCTCC 2 cut(s) 157, 1329
Lsp1109I GCAGC 5 cut(s) 182, 899, 902, 1282, 1285
LweI GCATC 3 cut(s) 157, 649, 1251
MaeI CTAG 1 cut(s) 585
MaeII ACGT 2 cut(s) 69, 1122
MaeIII GTNAC 4 cut(s) 803, 1118, 1156, 1277
MalI GATC 6 cut(s) 9, 37, 107, 399, 813, 873
MboI GATC 6 cut(s) 7, 35, 105, 397, 811, 871
MboII GAAGA 5 cut(s) 232, 295, 359, 668, 775
MfeI CAATTG 1 cut(s) 1333
MflI RGATCY 2 cut(s) 7, 397
MlyI GAGTC 3 cut(s) 488, 834, 1099
MmeI TCCRAC 1 cut(s) 670
MseI TTAA 8 cut(s) 317, 533, 683, 903, 977, 1011, 1065, 1080
MslI CAYNNNNRTG 1 cut(s) 550
MspA1I CMGCKG 1 cut(s) 173
MspCI CTTAAG 1 cut(s) 976
MspI CCGG 3 cut(s) 209, 326, 593
MspR9I CCNGG 3 cut(s) 594, 1100, 1154
MunI CAATTG 1 cut(s) 1333
MvaI CCWGG 2 cut(s) 1100, 1154
MwoI GCNNNNNNNGC 2 cut(s) 176, 205
NciI CCSGG 1 cut(s) 594
NcoI CCATGG 1 cut(s) 545
NdeI CATATG 1 cut(s) 94
NdeII GATC 6 cut(s) 7, 35, 105, 397, 811, 871
NlaIII CATG 7 cut(s) 283, 340, 382, 549, 1052, 1144, 1220
NlaIV GGNNCC 4 cut(s) 9, 124, 399, 1149
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 2 cut(s) 1118, 1156
NspI RCATGY 2 cut(s) 340, 1052
PaeI GCATGC 1 cut(s) 1052
PaeR7I CTCGAG 1 cut(s) 28
PagI TCATGA 1 cut(s) 378
PflMI CCANNNNNTGG 1 cut(s) 258
PkrI GCNGC 5 cut(s) 172, 889, 892, 1297, 1300
PleI GAGTC 3 cut(s) 487, 834, 1098
PpsI GAGTC 3 cut(s) 487, 834, 1098
PpuMI RGGWCCY 1 cut(s) 1147
Psp5II RGGWCCY 1 cut(s) 1147
Psp6I CCWGG 2 cut(s) 1098, 1152
PspGI CCWGG 2 cut(s) 1098, 1152
PspN4I GGNNCC 4 cut(s) 9, 124, 399, 1149
PspPI GGNCC 3 cut(s) 273, 463, 1147
PspPPI RGGWCCY 1 cut(s) 1147
PspXI VCTCGAGB 1 cut(s) 28
PsrI GAACNNNNNNTAC 2 cut(s) 1116, 1148
PsuI RGATCY 2 cut(s) 7, 397
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 4 cut(s) 25, 335, 741, 1193
RsaNI GTAC 4 cut(s) 24, 334, 740, 1192
RseI CAYNNNNRTG 1 cut(s) 550
SaqAI TTAA 8 cut(s) 317, 533, 683, 903, 977, 1011, 1065, 1080
SatI GCNGC 5 cut(s) 171, 888, 891, 1296, 1299
Sau3AI GATC 6 cut(s) 7, 35, 105, 397, 811, 871
Sau96I GGNCC 3 cut(s) 273, 463, 1147
SchI GAGTC 3 cut(s) 488, 834, 1099
ScrFI CCNGG 3 cut(s) 594, 1100, 1154
SfaNI GCATC 3 cut(s) 157, 649, 1251
Sfr274I CTCGAG 1 cut(s) 28
SinI GGWCC 2 cut(s) 273, 1147
SlaI CTCGAG 1 cut(s) 28
SmiI ATTTAAAT 1 cut(s) 1012
SmiMI CAYNNNNRTG 1 cut(s) 550
SmlI CTYRAG 4 cut(s) 28, 725, 976, 1232
SmoI CTYRAG 4 cut(s) 28, 725, 976, 1232
SphI GCATGC 1 cut(s) 1052
SsiI CCGC 4 cut(s) 74, 395, 837, 1246
SspI AATATT 3 cut(s) 1008, 1016, 1312
SspMI CTAG 1 cut(s) 585
StyD4I CCNGG 3 cut(s) 592, 1098, 1152
StyI CCWWGG 2 cut(s) 252, 545
SwaI ATTTAAAT 1 cut(s) 1012
TaaI ACNGT 4 cut(s) 681, 688, 803, 1114
TaiI ACGT 2 cut(s) 72, 1125
TaqI TCGA 1 cut(s) 29
TatI WGTACW 2 cut(s) 333, 739
Tru1I TTAA 8 cut(s) 317, 533, 683, 903, 977, 1011, 1065, 1080
Tru9I TTAA 8 cut(s) 317, 533, 683, 903, 977, 1011, 1065, 1080
TscAI CASTG 4 cut(s) 18, 294, 489, 1123
TseFI GTSAC 2 cut(s) 1118, 1156
TseI GCWGC 5 cut(s) 170, 887, 890, 1295, 1298
Tsp45I GTSAC 2 cut(s) 1118, 1156
TspDTI ATGAA 6 cut(s) 81, 153, 296, 681, 1089, 1233
TspGWI ACGGA 2 cut(s) 11, 76
TspRI CASTG 4 cut(s) 18, 294, 489, 1123
Van91I CCANNNNNTGG 1 cut(s) 258
Vha464I CTTAAG 1 cut(s) 976
VpaK11BI GGWCC 2 cut(s) 273, 1147
XapI RAATTY 5 cut(s) 82, 140, 927, 1067, 1076
XceI RCATGY 2 cut(s) 340, 1052
XcmI CCANNNNNNNNNTGG 1 cut(s) 1180
XhoI CTCGAG 1 cut(s) 28
XspI CTAG 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.