Rw3G025690

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002805
Physical Location & Seq
Forward (+)
34532187 .. 34533005
819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025690.1

Sequence Viewer

Length: 390 bp
ATGAGGGATCCACCGGCGTTTCGTACCTCGAGCGGATCAGCCAAGAGAACCAAATCAACCAAACGCTACGTTCCGCAGAAGAATTCCGTGGCGTATGCTTTGTTGATCACGCTTTACAGGGGAACCGAAAATGGAGATGAATTTATGCGTAAACAGGAGCTTATTGATGCAGCTGAAGCTAGTGGACTTTCTCGTGTGCCGATTATGCCGGAGAAGGGGAAGGGAAAGCCTACAACTCTCGGCAATTCTGCCAAGGAATGGTATAGTGGTTGGTCCTGCATGACGACATTGATAACCAAAGGACTGGTTCTGAAATTAAGTTGTCCGGCAAAGTATGGCTTTTGCTGTTACATTACTTTTTCGTTTGGTCATGCTAAAAAGCAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

129

Amino Acids

14.27

Weight (kDa)

9.78

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_MUS81 PF21136 25 - 113 2.7e-10 Crossover junction endonuclease MUS81-like, winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 258
AccBSI CCGCTC 1 cut(s) 33
AciI CCGC 2 cut(s) 33, 74
AclWI GGATC 3 cut(s) 2, 15, 43
AcsI RAATTY 2 cut(s) 82, 140
AcuI CTGAAG 1 cut(s) 195
AfaI GTAC 1 cut(s) 25
AfiI CCNNNNNNNGG 2 cut(s) 215, 258
AluBI AGCT 3 cut(s) 160, 173, 179
AluI AGCT 3 cut(s) 160, 173, 179
AlwI GGATC 3 cut(s) 2, 15, 43
Ama87I CYCGRG 1 cut(s) 28
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 2 cut(s) 82, 140
AspS9I GGNCC 1 cut(s) 273
AvaI CYCGRG 1 cut(s) 28
AvaII GGWCC 1 cut(s) 273
BamHI GGATCC 1 cut(s) 7
BauI CACGAG 1 cut(s) 192
BbvI GCAGC 1 cut(s) 182
BclI TGATCA 1 cut(s) 105
BfaI CTAG 1 cut(s) 180
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
Bme18I GGWCC 1 cut(s) 273
BmeT110I CYCGRG 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 2 cut(s) 9, 124
BmsI GCATC 1 cut(s) 157
BsaJI CCNNGG 2 cut(s) 87, 252
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 258
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 1 cut(s) 309
BseDI CCNNGG 2 cut(s) 87, 252
BseLI CCNNNNNNNGG 2 cut(s) 215, 258
BseNI ACTGG 1 cut(s) 309
BseXI GCAGC 1 cut(s) 182
BsiHKCI CYCGRG 1 cut(s) 28
BsiSI CCGG 3 cut(s) 14, 209, 326
BslI CCNNNNNNNGG 2 cut(s) 215, 258
BsoBI CYCGRG 1 cut(s) 28
Bsp143I GATC 3 cut(s) 7, 35, 105
BspACI CCGC 2 cut(s) 33, 74
BspLI GGNNCC 2 cut(s) 9, 124
BspPI GGATC 3 cut(s) 2, 15, 43
BsrBI CCGCTC 1 cut(s) 33
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 1 cut(s) 309
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 87, 252
BssMI GATC 3 cut(s) 7, 35, 105
BssSI CACGAG 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 252
Bst2BI CACGAG 1 cut(s) 192
BstDSI CCRYGG 1 cut(s) 87
BstKTI GATC 3 cut(s) 10, 38, 108
BstMBI GATC 3 cut(s) 7, 35, 105
BstMWI GCNNNNNNNGC 2 cut(s) 176, 205
BstV1I GCAGC 1 cut(s) 182
BstX2I RGATCY 1 cut(s) 7
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 1 cut(s) 7
BtgI CCRYGG 1 cut(s) 87
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 1 cut(s) 273
Csp6I GTAC 1 cut(s) 24
CviAII CATG 2 cut(s) 280, 371
CviJI RGCY 6 cut(s) 41, 160, 173, 179, 229, 339
CviKI_1 RGCY 6 cut(s) 41, 160, 173, 179, 229, 339
CviQI GTAC 1 cut(s) 24
DpnI GATC 3 cut(s) 9, 37, 107
DpnII GATC 3 cut(s) 7, 35, 105
Eco130I CCWWGG 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 273
Eco57I CTGAAG 1 cut(s) 195
Eco88I CYCGRG 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 2 cut(s) 283, 374
FaiI YATR 8 cut(s) 96, 146, 206, 264, 281, 336, 372, 388
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FatI CATG 2 cut(s) 279, 370
FbaI TGATCA 1 cut(s) 105
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 1 cut(s) 180
GluI GCNGC 1 cut(s) 171
HapII CCGG 3 cut(s) 14, 209, 326
Hin1II CATG 2 cut(s) 283, 374
HpaII CCGG 3 cut(s) 14, 209, 326
Hpy166II GTNNAC 2 cut(s) 152, 185
Hpy188I TCNGA 1 cut(s) 312
Hpy8I GTNNAC 2 cut(s) 152, 185
HpyAV CCTTC 2 cut(s) 208, 214
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 2 cut(s) 170, 279
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 205
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 2 cut(s) 283, 374
Ksp22I TGATCA 1 cut(s) 105
Kzo9I GATC 3 cut(s) 7, 35, 105
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 7 cut(s) 27, 103, 140, 222, 289, 290, 339
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 1 cut(s) 157
MaeI CTAG 1 cut(s) 180
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 347
MalI GATC 3 cut(s) 9, 37, 107
MbiI CCGCTC 1 cut(s) 33
MboI GATC 3 cut(s) 7, 35, 105
MboII GAAGA 1 cut(s) 91
MflI RGATCY 1 cut(s) 7
MluCI AATT 4 cut(s) 82, 140, 244, 314
MnlI CCTC 1 cut(s) 37
MseI TTAA 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 3 cut(s) 14, 209, 326
MwoI GCNNNNNNNGC 2 cut(s) 176, 205
NdeII GATC 3 cut(s) 7, 35, 105
NlaIII CATG 2 cut(s) 283, 374
NlaIV GGNNCC 2 cut(s) 9, 124
NmeAIII GCCGAG 1 cut(s) 219
PaeR7I CTCGAG 1 cut(s) 28
PflMI CCANNNNNTGG 1 cut(s) 258
PkrI GCNGC 1 cut(s) 172
PspN4I GGNNCC 2 cut(s) 9, 124
PspPI GGNCC 1 cut(s) 273
PspXI VCTCGAGB 1 cut(s) 28
PsuI RGATCY 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 1 cut(s) 25
RsaNI GTAC 1 cut(s) 24
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 3 cut(s) 7, 35, 105
Sau96I GGNCC 1 cut(s) 273
SetI ASST 5 cut(s) 29, 72, 162, 175, 181
SfaNI GCATC 1 cut(s) 157
Sfr274I CTCGAG 1 cut(s) 28
SgrAI CRCCGGYG 1 cut(s) 13
SinI GGWCC 1 cut(s) 273
SlaI CTCGAG 1 cut(s) 28
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
Sse9I AATT 4 cut(s) 82, 140, 244, 314
SsiI CCGC 2 cut(s) 33, 74
SspMI CTAG 1 cut(s) 180
StyI CCWWGG 1 cut(s) 252
TaiI ACGT 1 cut(s) 72
TaqI TCGA 1 cut(s) 29
TasI AATT 4 cut(s) 82, 140, 244, 314
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 153
TspGWI ACGGA 1 cut(s) 76
Van91I CCANNNNNTGG 1 cut(s) 258
VpaK11BI GGWCC 1 cut(s) 273
XapI RAATTY 2 cut(s) 82, 140
XhoI CTCGAG 1 cut(s) 28
XspI CTAG 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.