Rh3BG327000

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002805
Physical Location & Seq
Forward (+)
34079954 .. 34083736
3783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG327000.1

Sequence Viewer

Length: 948 bp
ATGAGGGATCCACCGGCGTTTCGTACCTCGAGCGGATCAGCCAAGAGAACCAAATCAACCAAACGCTACGTTCCGCAGAAGAATTCCGTGGCATATGCTTTGTTGATCACGCTCTACAGGGGAGCCGAAAATGGAGATGAATTTATGCGTAAGCAGGAGCTTATTGATGCAGCTGAAGCAAGTGGACTTTCTCGTGTGCCGATTATGCCGGAGAAGGGGAAGGGAAAACCTACAACTCTCGGCAATTCTGCCAAGGAATGGTATAGTGGTTGGTCCTGCATGAAGACATTGATAACCAAAGGACTGGTTCTGAAATCAAGTTGCCCCGCAAAGTACATGCTTACTGAAGAAGGTCAGGAAGTTGCCCGTGAATGTCTCATGAGGTCTGGTTTGGCGGATCCTGTGGACAAAGTTTCTAATGCCAAAGGGTTGTCTGTCGTGGATGTGAATGTATCAGAACAGGACCTTTCTCATCCTGAGTCAGTGACAGATATGACATTGTTGTCCAGTAATTCAAGTAGTGAGGAGAACTTAATTGATATGCCATGGGAATGTCTTGACAAGAGTTGTCCCGTGCCAAAGTCTAGTTCTCCGGGTGTCAAAGCAAGTTCAAAAGCTTTATCCATTCCACCTCTAAGCTTTGGGGAGAGATTTGAAGATGCTTATGAAGTAGTCTTTCTATTAGATGACCGAGAGCAATTGACCGCTCAGATATTGCGGTCTAGGAGCATTATTGAGAGTGTTATTTCTGAATCCAAAATTAAAGTAGAGGTCAGACGGTTACCAGTCGGAGATGGGATTTGGATAGCTCGCCATAATTATCTTGAGACTGAATATGTACTTGATTTTATTGTTGAGAGGAAGAATGTTGATGATTTACGCTGTTCAATAAGAGACAACCGTTACAGGGATCAAAAATTGAGACTTTTGGTATGGAGTTCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

315

Amino Acids

35.23

Weight (kDa)

7.53

Isoelectric Point (pI)

53.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_MUS81 PF21136 25 - 123 1.1e-13 Crossover junction endonuclease MUS81-like, winged helix domain
ERCC4 PF02732 229 - 309 1.7e-13 ERCC4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 502
AccB7I CCANNNNNTGG 1 cut(s) 258
AccBSI CCGCTC 2 cut(s) 33, 707
AciI CCGC 6 cut(s) 33, 74, 327, 395, 705, 718
AclWI GGATC 6 cut(s) 2, 15, 43, 392, 405, 918
AcsI RAATTY 2 cut(s) 82, 140
AcuI CTGAAG 2 cut(s) 195, 366
AfaI GTAC 3 cut(s) 25, 335, 840
AfiI CCNNNNNNNGG 3 cut(s) 215, 258, 907
AgsI TTSAA 4 cut(s) 516, 612, 656, 888
AjuI GAANNNNNNNTTGG 2 cut(s) 571, 603
AluBI AGCT 5 cut(s) 160, 173, 617, 639, 809
AluI AGCT 5 cut(s) 160, 173, 617, 639, 809
Alw26I GTCTC 4 cut(s) 380, 821, 888, 916
AlwI GGATC 6 cut(s) 2, 15, 43, 392, 405, 918
Ama87I CYCGRG 1 cut(s) 28
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 2 cut(s) 82, 140
AspS9I GGNCC 2 cut(s) 273, 463
AsuC2I CCSGG 1 cut(s) 594
AvaI CYCGRG 1 cut(s) 28
AvaII GGWCC 2 cut(s) 273, 463
BamHI GGATCC 2 cut(s) 7, 397
BauI CACGAG 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 290
BbvI GCAGC 1 cut(s) 182
BccI CCATC 1 cut(s) 788
BclI TGATCA 1 cut(s) 105
BcnI CCSGG 1 cut(s) 594
BcoDI GTCTC 4 cut(s) 380, 821, 888, 916
BfaI CTAG 2 cut(s) 585, 723
BfmI CTRYAG 1 cut(s) 115
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
Bme1390I CCNGG 1 cut(s) 594
Bme18I GGWCC 2 cut(s) 273, 463
BmeT110I CYCGRG 1 cut(s) 28
BmgT120I GGNCC 2 cut(s) 273, 463
BmiI GGNNCC 3 cut(s) 9, 124, 399
BmrFI CCNGG 1 cut(s) 594
BmsI GCATC 2 cut(s) 157, 649
BpiI GAAGAC 1 cut(s) 290
BpuEI CTTGAG 1 cut(s) 845
BpuMI CCSGG 1 cut(s) 594
BsaJI CCNNGG 3 cut(s) 87, 252, 545
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 258, 907
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 3 cut(s) 309, 507, 785
BseDI CCNNGG 3 cut(s) 87, 252, 545
BseGI GGATG 2 cut(s) 448, 472
BseLI CCNNNNNNNGG 3 cut(s) 215, 258, 907
BseMII CTCAG 2 cut(s) 468, 722
BseNI ACTGG 3 cut(s) 309, 507, 785
BseRI GAGGAG 1 cut(s) 539
BseXI GCAGC 1 cut(s) 182
BsiHKCI CYCGRG 1 cut(s) 28
BsiSI CCGG 3 cut(s) 14, 209, 593
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 3 cut(s) 215, 258, 907
BsmAI GTCTC 4 cut(s) 380, 821, 888, 916
BsmFI GGGAC 1 cut(s) 555
BsoBI CYCGRG 1 cut(s) 28
Bsp143I GATC 5 cut(s) 7, 35, 105, 397, 910
Bsp19I CCATGG 1 cut(s) 545
BspACI CCGC 6 cut(s) 33, 74, 327, 395, 705, 718
BspCNI CTCAG 2 cut(s) 469, 721
BspHI TCATGA 1 cut(s) 378
BspLI GGNNCC 3 cut(s) 9, 124, 399
BspPI GGATC 6 cut(s) 2, 15, 43, 392, 405, 918
BsrBI CCGCTC 2 cut(s) 33, 707
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 3 cut(s) 309, 507, 785
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 3 cut(s) 87, 252, 545
BssMI GATC 5 cut(s) 7, 35, 105, 397, 910
BssSI CACGAG 1 cut(s) 192
BssT1I CCWWGG 2 cut(s) 252, 545
Bst2BI CACGAG 1 cut(s) 192
Bst4CI ACNGT 2 cut(s) 780, 902
BstC8I GCNNGC 1 cut(s) 811
BstDEI CTNAG 3 cut(s) 477, 635, 708
BstDSI CCRYGG 2 cut(s) 87, 545
BstEII GGTNACC 1 cut(s) 780
BstF5I GGATG 2 cut(s) 448, 472
BstKTI GATC 5 cut(s) 10, 38, 108, 400, 913
BstMAI GTCTC 4 cut(s) 380, 821, 888, 916
BstMBI GATC 5 cut(s) 7, 35, 105, 397, 910
BstMWI GCNNNNNNNGC 2 cut(s) 176, 205
BstNSI RCATGY 1 cut(s) 340
BstPI GGTNACC 1 cut(s) 780
BstSCI CCNGG 1 cut(s) 592
BstSFI CTRYAG 1 cut(s) 115
BstV1I GCAGC 1 cut(s) 182
BstV2I GAAGAC 1 cut(s) 290
BstX2I RGATCY 2 cut(s) 7, 397
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 2 cut(s) 7, 397
BtgI CCRYGG 2 cut(s) 87, 545
BtsCI GGATG 2 cut(s) 448, 472
BtsIMutI CAGTG 1 cut(s) 489
Cac8I GCNNGC 1 cut(s) 811
CciI TCATGA 1 cut(s) 378
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 2 cut(s) 273, 463
Csp6I GTAC 3 cut(s) 24, 334, 839
CviAII CATG 4 cut(s) 280, 337, 379, 546
CviJI RGCY 7 cut(s) 41, 125, 160, 173, 617, 639, 809
CviKI_1 RGCY 7 cut(s) 41, 125, 160, 173, 617, 639, 809
CviQI GTAC 3 cut(s) 24, 334, 839
DdeI CTNAG 3 cut(s) 477, 635, 708
DpnI GATC 5 cut(s) 9, 37, 107, 399, 912
DpnII GATC 5 cut(s) 7, 35, 105, 397, 910
DrdI GACNNNNNNGTC 1 cut(s) 502
DseDI GACNNNNNNGTC 1 cut(s) 502
EciI GGCGGA 1 cut(s) 410
Eco130I CCWWGG 2 cut(s) 252, 545
Eco47I GGWCC 2 cut(s) 273, 463
Eco57I CTGAAG 2 cut(s) 195, 366
Eco88I CYCGRG 1 cut(s) 28
Eco91I GGTNACC 1 cut(s) 780
EcoO109I RGGNCCY 1 cut(s) 463
EcoO65I GGTNACC 1 cut(s) 780
EcoRI GAATTC 1 cut(s) 82
EcoT14I CCWWGG 2 cut(s) 252, 545
ErhI CCWWGG 2 cut(s) 252, 545
FaeI CATG 4 cut(s) 283, 340, 382, 549
FaqI GGGAC 1 cut(s) 555
FatI CATG 4 cut(s) 279, 336, 378, 545
FauI CCCGC 1 cut(s) 334
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 1 cut(s) 105
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 2 cut(s) 455, 459
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 2 cut(s) 585, 723
GluI GCNGC 1 cut(s) 171
HapII CCGG 3 cut(s) 14, 209, 593
Hin1II CATG 4 cut(s) 283, 340, 382, 549
HindIII AAGCTT 2 cut(s) 615, 637
HinfI GANTC 2 cut(s) 479, 752
HpaII CCGG 3 cut(s) 14, 209, 593
Hpy166II GTNNAC 2 cut(s) 185, 406
Hpy188I TCNGA 6 cut(s) 312, 457, 711, 751, 776, 791
Hpy188III TCNNGA 5 cut(s) 356, 379, 476, 557, 824
Hpy8I GTNNAC 2 cut(s) 185, 406
HpyAV CCTTC 3 cut(s) 208, 214, 344
HpyCH4III ACNGT 2 cut(s) 780, 902
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 2 cut(s) 170, 279
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 205
HpyF3I CTNAG 3 cut(s) 477, 635, 708
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 4 cut(s) 283, 340, 382, 549
Ksp22I TGATCA 1 cut(s) 105
Kzo9I GATC 5 cut(s) 7, 35, 105, 397, 910
LmnI GCTCC 3 cut(s) 122, 157, 726
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 2 cut(s) 157, 649
MaeI CTAG 2 cut(s) 585, 723
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 3 cut(s) 484, 780, 902
MalI GATC 5 cut(s) 9, 37, 107, 399, 912
MbiI CCGCTC 2 cut(s) 33, 707
MboI GATC 5 cut(s) 7, 35, 105, 397, 910
MboII GAAGA 5 cut(s) 91, 295, 359, 668, 874
MfeI CAATTG 1 cut(s) 698
MflI RGATCY 2 cut(s) 7, 397
MluCI AATT 9 cut(s) 82, 140, 244, 511, 534, 698, 759, 817, 917
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 769
MnlI CCTC 6 cut(s) 37, 375, 517, 642, 763, 852
MseI TTAA 2 cut(s) 533, 762
MslI CAYNNNNRTG 1 cut(s) 550
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 3 cut(s) 14, 209, 593
MspR9I CCNGG 1 cut(s) 594
MunI CAATTG 1 cut(s) 698
MwoI GCNNNNNNNGC 2 cut(s) 176, 205
NciI CCSGG 1 cut(s) 594
NcoI CCATGG 1 cut(s) 545
NdeI CATATG 1 cut(s) 94
NdeII GATC 5 cut(s) 7, 35, 105, 397, 910
NlaIII CATG 4 cut(s) 283, 340, 382, 549
NlaIV GGNNCC 3 cut(s) 9, 124, 399
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 1 cut(s) 484
NspI RCATGY 1 cut(s) 340
PaeR7I CTCGAG 1 cut(s) 28
PagI TCATGA 1 cut(s) 378
PfeI GAWTC 1 cut(s) 752
PflMI CCANNNNNTGG 1 cut(s) 258
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
PpuMI RGGWCCY 1 cut(s) 463
Psp5II RGGWCCY 1 cut(s) 463
PspEI GGTNACC 1 cut(s) 780
PspN4I GGNNCC 3 cut(s) 9, 124, 399
PspPI GGNCC 2 cut(s) 273, 463
PspPPI RGGWCCY 1 cut(s) 463
PspXI VCTCGAGB 1 cut(s) 28
PsuI RGATCY 2 cut(s) 7, 397
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 3 cut(s) 25, 335, 840
RsaNI GTAC 3 cut(s) 24, 334, 839
RseI CAYNNNNRTG 1 cut(s) 550
SaqAI TTAA 2 cut(s) 533, 762
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 5 cut(s) 7, 35, 105, 397, 910
Sau96I GGNCC 2 cut(s) 273, 463
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 1 cut(s) 594
SfaNI GCATC 2 cut(s) 157, 649
SfcI CTRYAG 1 cut(s) 115
Sfr274I CTCGAG 1 cut(s) 28
SgrAI CRCCGGYG 1 cut(s) 13
SinI GGWCC 2 cut(s) 273, 463
SlaI CTCGAG 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 550
SmlI CTYRAG 2 cut(s) 28, 824
SmoI CTYRAG 2 cut(s) 28, 824
Sse9I AATT 9 cut(s) 82, 140, 244, 511, 534, 698, 759, 817, 917
SsiI CCGC 6 cut(s) 33, 74, 327, 395, 705, 718
SspMI CTAG 2 cut(s) 585, 723
StyD4I CCNGG 1 cut(s) 592
StyI CCWWGG 2 cut(s) 252, 545
TaaI ACNGT 2 cut(s) 780, 902
TaiI ACGT 1 cut(s) 72
TaqI TCGA 1 cut(s) 29
TaqII GACCGA 1 cut(s) 705
TasI AATT 9 cut(s) 82, 140, 244, 511, 534, 698, 759, 817, 917
TatI WGTACW 2 cut(s) 333, 838
TfiI GAWTC 1 cut(s) 752
Tru1I TTAA 2 cut(s) 533, 762
Tru9I TTAA 2 cut(s) 533, 762
TscAI CASTG 1 cut(s) 489
TseFI GTSAC 1 cut(s) 484
TseI GCWGC 1 cut(s) 170
Tsp45I GTSAC 1 cut(s) 484
TspDTI ATGAA 3 cut(s) 153, 296, 681
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 1 cut(s) 489
Van91I CCANNNNNTGG 1 cut(s) 258
VpaK11BI GGWCC 2 cut(s) 273, 463
XapI RAATTY 2 cut(s) 82, 140
XceI RCATGY 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 28
XspI CTAG 2 cut(s) 585, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.