MD02G1296200.v1.1

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002805
Physical Location & Seq
Reverse (-)
35118795 .. 35126923
8129 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1296200.v1.1.491

Sequence Viewer

Length: 2022 bp
ATGGAGCGGCAGAAGCGCACGGTGGTTTGCCCGGAGAACGAAGAGCTTGCGACGTACATGCTGAGCAAGTGGCAGGAAATGGCCGATCAAAGGCCCAAGGGCATTTCCGAGAACATCGAAATGACTCTTTCCAAGGCTTACGCCAACCTCTGCAAATCCAAGAACCCTATTAAAACCCTCAAAGATTTTTCCCAAATCAAGGGCATAGGGAAGTGGATTTTGAAGCTTATGAAAGGATTTTTTGATACTGCTTCAGACAATTCTGAACCAGAAGACATAACTAAAAAGGGTAAGAGAACCAAAGGAAGCAGACGTTATGTTCCACAAAAGAATTCTGTATCGTATGCATTGTTGATCACACTTTACAGGGGAATTGAAAGTGGGAATGAATTTATGCGTAAACAGGAGCTTATTGATGCTGCTGAAGCAAGTGGACTTTCTCGTGTGCCAATTATGCCAGAGAAGGGAAAGGGAAAACCTGGACACTTTGCAACTTCTGCAAGGGAATGGTATAGTGGATGGTCCTGCATGACGACATTAATAAACAAGGGACTGGTTGTGAAATCAAGTAACCCAGCAAAGTACATGCTTACTCCAGAAGGTAAGGAAACTGCAAGTGAATGTCTCATGAGGTCTGGTATGGAAAAACCTGTAGGAAAGACTTCTCATGCCACAGGGCTTTCTGGAAATGGGGACAATATTTCACATCAGGAGCTTGCTCATTATGACTCAGCCACTTTGGCGACATTGGTCTCCACTAATTTGAGTACAAAGGAGAAATCCTTTGATATTCCACAGGAATACCTTGACAAGTATATGTGCATGGGGTATTCTAGAGAACAAATTGTTCAAGCTTTTACTGAAGTTTCGGAAACTTCCAATGACAAGGAAATGTCTTCACTATGGCCATCTGTTCTGTGTCGTCTTCGAGAAGATGAAGTTTATGGTTTGCATTTGAAGTCTCAAACTTTGAGGGAAGATCTTTGTCCAGCATCAGTCACTCATGTGGTTGCAAATGGTCTTAGAGATTTTGTAGCGAGTGAGAATAGACTTGCCAAATTTTCTCTCGATGGTGGACATGTGCCAAAAATTAATTCGGATGAGTCATTACCAAAATCTTTTACGCTTAGAGCTTGTTCTTCCACGAGTCATTCTGTGCCAAAGTCATGTTCGAATGGATTGGAAGCAAGTTCGAATGTTTTATCTATACCACCGCTAAGCTTTGGTGAGAGATTTGAGGAAGCATATGAAGTAATCTTGGTACTGGATGATCGGGAGCAATTTGCCACTCAGGGATCGCGATCTAGGAGACTTATCGAGAACGTTCGTACTCAATTCAAAATAAAAATAGAGGTTAGGAGGTTACCAGTTGGAGATGGAATCTGGATAGCTCGTCATAAACAACTCGAGAGTGAATATGTACTTGATTTTATTGTTGAGAGGAAGAATGTTGATGATTTGCGCTGTTCAATCAGAGATAACCGTTACAGGGATCAGAAATTGAGACTTTTGAGGTGTGGACTCAAGAAACTGATATATCTTGTGGAAGGTGATCCAAATACTTCTGAAGCAGCAGAAAGCATTAAAACAGCCTGTTTCACAACTGAGATTTTGGAGGGATTTGACGTCCAGAGAACCATTGGCTTGGCTGATACATTGAAGAAGTATGTTTATCTAACTCAAGCAATAGCTCAGTATTACAAATCAGAGTTCTCCGAGGAACAATGCAAACACGCTGGGGTTTGTCCTCCTTTTGATGAATTTATTAGAAGGTGCCAAGACTTGGATAAAATGACAGTCAGCGACGTTTTTGCCGTTCAGCTCATGCAGGTCCCACAGGTAACTGAGGAAGTCGCCATAGCTGTTCTGGATATGTACCCGACACTTTTATCTCTTGCTCGTGCCTACTCTTTACTTGAGGGTGATTTACGTGCACAAGAGGGGATGCTTAGAACACAGAGTAACAATGCCGTCAATGCGAGCGCTAGTAAGAATATATTTCAATTGGTTTGGGGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

674

Amino Acids

75.76

Weight (kDa)

7.47

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_MUS81 PF21136 108 - 205 5.2e-12 Crossover junction endonuclease MUS81-like, winged helix domain
ERCC4 PF02732 423 - 554 1.4e-22 ERCC4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1629
Acc36I ACCTGC 1 cut(s) 1819
AccB1I GGYRCC 1 cut(s) 1773
AccB7I CCANNNNNTGG 1 cut(s) 1783
AccBSI CCGCTC 1 cut(s) 7
AccII CGCG 1 cut(s) 1300
AciI CCGC 2 cut(s) 7, 1214
AclI AACGTT 1 cut(s) 1323
AclWI GGATC 3 cut(s) 1303, 1500, 1547
AcoI YGGCCR 2 cut(s) 81, 905
AcsI RAATTY 4 cut(s) 331, 389, 1058, 1760
AcuI CTGAAG 4 cut(s) 237, 444, 882, 1587
AcyI GRCGYC 1 cut(s) 1626
AfaI GTAC 7 cut(s) 56, 584, 769, 1263, 1330, 1422, 1877
AfeI AGCGCT 1 cut(s) 1984
AfiI CCNNNNNNNGG 5 cut(s) 90, 199, 464, 1489, 1783
AflIII ACRYGT 1 cut(s) 1078
AgsI TTSAA 8 cut(s) 223, 377, 851, 958, 1339, 1470, 1660, 2003
AjnI CCWGG 1 cut(s) 478
AjuI GAANNNNNNNTTGG 4 cut(s) 1078, 1110, 1153, 1185
AleI CACNNNNGTG 1 cut(s) 1004
Alw21I GWGCWC 1 cut(s) 1936
Alw26I GTCTC 5 cut(s) 629, 757, 966, 1303, 1498
Alw44I GTGCAC 1 cut(s) 1932
AlwI GGATC 3 cut(s) 1303, 1500, 1547
Ama87I CYCGRG 1 cut(s) 1406
Aor51HI AGCGCT 1 cut(s) 1984
AoxI GGCC 3 cut(s) 81, 92, 905
ApaLI GTGCAC 1 cut(s) 1932
ApeKI GCWGC 2 cut(s) 419, 1571
ApoI RAATTY 4 cut(s) 331, 389, 1058, 1760
AseI ATTAAT 2 cut(s) 539, 1092
Asp700I GAANNNNTTC 1 cut(s) 661
AspLEI GCGC 3 cut(s) 18, 1464, 1985
AspS9I GGNCC 3 cut(s) 93, 522, 1831
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 3 cut(s) 1238, 1562, 1934
AsuII TTCGAA 2 cut(s) 1172, 1193
AvaI CYCGRG 1 cut(s) 1406
AvaII GGWCC 2 cut(s) 522, 1831
BaeGI GKGCMC 1 cut(s) 1936
BalI TGGCCA 1 cut(s) 907
BanI GGYRCC 1 cut(s) 1773
BauI CACGAG 3 cut(s) 441, 1144, 1899
BbsI GAAGAC 3 cut(s) 279, 888, 917
Bbv12I GWGCWC 1 cut(s) 1936
BbvI GCAGC 2 cut(s) 406, 1583
BccI CCATC 4 cut(s) 513, 916, 1064, 1370
BceAI ACGGC 2 cut(s) 1799, 1955
BcgI CGANNNNNNTGC 6 cut(s) 29, 40, 63, 74, 1793, 1827
BciT130I CCWGG 1 cut(s) 480
BclI TGATCA 1 cut(s) 354
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 5 cut(s) 629, 757, 966, 1303, 1498
BfaI CTAG 3 cut(s) 834, 1305, 1986
BfmI CTRYAG 1 cut(s) 651
BfoI RGCGCY 1 cut(s) 1986
BfuAI ACCTGC 1 cut(s) 1819
BglI GCCNNNNNGGC 1 cut(s) 740
BglII AGATCT 1 cut(s) 979
BisI GCNGC 3 cut(s) 8, 420, 1572
BlpI GCTNAGC 2 cut(s) 62, 1217
BlsI GCNGC 3 cut(s) 9, 421, 1573
Bme1390I CCNGG 2 cut(s) 32, 480
Bme18I GGWCC 2 cut(s) 522, 1831
BmeT110I CYCGRG 1 cut(s) 1406
BmgT120I GGNCC 3 cut(s) 93, 522, 1831
BmiI GGNNCC 2 cut(s) 1775, 1833
BmrFI CCNGG 2 cut(s) 32, 480
BmsI GCATC 3 cut(s) 406, 1001, 1935
BoxI GACNNNNGTC 1 cut(s) 749
BpiI GAAGAC 3 cut(s) 279, 888, 917
BpmI CTGGAG 1 cut(s) 579
Bpu1102I GCTNAGC 2 cut(s) 62, 1217
Bpu14I TTCGAA 2 cut(s) 1172, 1193
BpuEI CTTGAG 3 cut(s) 1508, 1665, 1937
BpuMI CCSGG 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 1931
BsaBI GATNNNNATC 1 cut(s) 1300
BsaHI GRCGYC 1 cut(s) 1626
BsaI GGTCTC 1 cut(s) 757
BsaJI CCNNGG 3 cut(s) 96, 132, 1716
Bsc4I CCNNNNNNNGG 5 cut(s) 90, 199, 464, 1489, 1783
Bse1I ACTGG 3 cut(s) 558, 1269, 1367
Bse8I GATNNNNATC 1 cut(s) 1300
BseBI CCWGG 1 cut(s) 480
BseDI CCNNGG 3 cut(s) 96, 132, 1716
BseGI GGATG 4 cut(s) 524, 1105, 1273, 1950
BseJI GATNNNNATC 1 cut(s) 1300
BseLI CCNNNNNNNGG 5 cut(s) 90, 199, 464, 1489, 1783
BseMII CTCAG 6 cut(s) 53, 744, 1304, 1596, 1706, 1836
BseNI ACTGG 3 cut(s) 558, 1269, 1367
BseSI GKGCMC 1 cut(s) 1936
BseXI GCAGC 2 cut(s) 406, 1583
BseYI CCCAGC 2 cut(s) 574, 1736
Bsh1236I CGCG 1 cut(s) 1300
BshFI GGCC 3 cut(s) 83, 94, 907
BshNI GGYRCC 1 cut(s) 1773
BsiHKAI GWGCWC 1 cut(s) 1936
BsiHKCI CYCGRG 1 cut(s) 1406
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 3 cut(s) 564, 707, 1817
BslI CCNNNNNNNGG 5 cut(s) 90, 199, 464, 1489, 1783
BsmAI GTCTC 5 cut(s) 629, 757, 966, 1303, 1498
BsmFI GGGAC 3 cut(s) 564, 707, 1817
BsnI GGCC 3 cut(s) 83, 94, 907
Bso31I GGTCTC 1 cut(s) 757
BsoBI CYCGRG 1 cut(s) 1406
Bsp119I TTCGAA 2 cut(s) 1172, 1193
Bsp1286I GDGCHC 1 cut(s) 1936
Bsp143I GATC 8 cut(s) 85, 354, 979, 1270, 1295, 1301, 1492, 1552
Bsp1720I GCTNAGC 2 cut(s) 62, 1217
Bsp68I TCGCGA 1 cut(s) 1300
BspACI CCGC 2 cut(s) 7, 1214
BspANI GGCC 3 cut(s) 83, 94, 907
BspCNI CTCAG 6 cut(s) 54, 743, 1303, 1597, 1705, 1837
BspFNI CGCG 1 cut(s) 1300
BspHI TCATGA 1 cut(s) 627
BspLI GGNNCC 2 cut(s) 1775, 1833
BspMI ACCTGC 1 cut(s) 1819
BspPI GGATC 3 cut(s) 1303, 1500, 1547
BspQI GCTCTTC 1 cut(s) 36
BspT104I TTCGAA 2 cut(s) 1172, 1193
BspT107I GGYRCC 1 cut(s) 1773
BspTNI GGTCTC 1 cut(s) 757
BsrBI CCGCTC 1 cut(s) 7
BsrI ACTGG 3 cut(s) 558, 1269, 1367
BssECI CCNNGG 3 cut(s) 96, 132, 1716
BssMI GATC 8 cut(s) 85, 354, 979, 1270, 1295, 1301, 1492, 1552
BssNI GRCGYC 1 cut(s) 1626
BssSI CACGAG 3 cut(s) 441, 1144, 1899
BssT1I CCWWGG 2 cut(s) 96, 132
Bst2BI CACGAG 3 cut(s) 441, 1144, 1899
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 3 cut(s) 22, 1484, 1798
Bst6I CTCTTC 1 cut(s) 36
BstACI GRCGYC 1 cut(s) 1626
BstAPI GCANNNNNTGC 1 cut(s) 497
BstBAI YACGTR 1 cut(s) 1931
BstBI TTCGAA 2 cut(s) 1172, 1193
BstC8I GCNNGC 3 cut(s) 48, 717, 1981
BstEII GGTNACC 1 cut(s) 1362
BstF5I GGATG 4 cut(s) 524, 1105, 1273, 1950
BstFNI CGCG 1 cut(s) 1300
BstH2I RGCGCY 1 cut(s) 1986
BstHHI GCGC 3 cut(s) 18, 1464, 1985
BstKTI GATC 8 cut(s) 88, 357, 982, 1273, 1298, 1304, 1495, 1555
BstMAI GTCTC 5 cut(s) 629, 757, 966, 1303, 1498
BstMBI GATC 8 cut(s) 85, 354, 979, 1270, 1295, 1301, 1492, 1552
BstMWI GCNNNNNNNGC 6 cut(s) 13, 425, 454, 497, 740, 1976
BstNI CCWGG 1 cut(s) 480
BstNSI RCATGY 3 cut(s) 61, 589, 1082
BstPAI GACNNNNGTC 1 cut(s) 749
BstPI GGTNACC 1 cut(s) 1362
BstSCI CCNGG 2 cut(s) 30, 478
BstSFI CTRYAG 1 cut(s) 651
BstSLI GKGCMC 1 cut(s) 1936
BstUI CGCG 1 cut(s) 1300
BstV1I GCAGC 2 cut(s) 406, 1583
BstV2I GAAGAC 3 cut(s) 279, 888, 917
BstX2I RGATCY 1 cut(s) 979
BstXI CCANNNNNNTGG 1 cut(s) 1645
BstYI RGATCY 1 cut(s) 979
BsuRI GGCC 3 cut(s) 83, 94, 907
BtsCI GGATG 4 cut(s) 524, 1105, 1273, 1950
BtuMI TCGCGA 1 cut(s) 1300
BveI ACCTGC 1 cut(s) 1819
Cac8I GCNNGC 3 cut(s) 48, 717, 1981
CciI TCATGA 1 cut(s) 627
CfoI GCGC 3 cut(s) 18, 1464, 1985
Cfr13I GGNCC 3 cut(s) 93, 522, 1831
Csp6I GTAC 7 cut(s) 55, 583, 768, 1262, 1329, 1421, 1876
CviQI GTAC 7 cut(s) 55, 583, 768, 1262, 1329, 1421, 1876
DpnI GATC 8 cut(s) 87, 356, 981, 1272, 1297, 1303, 1494, 1554
DpnII GATC 8 cut(s) 85, 354, 979, 1270, 1295, 1301, 1492, 1552
EaeI YGGCCR 2 cut(s) 81, 905
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Eco130I CCWWGG 2 cut(s) 96, 132
Eco31I GGTCTC 1 cut(s) 757
Eco47I GGWCC 2 cut(s) 522, 1831
Eco47III AGCGCT 1 cut(s) 1984
Eco57I CTGAAG 4 cut(s) 237, 444, 882, 1587
Eco88I CYCGRG 1 cut(s) 1406
Eco91I GGTNACC 1 cut(s) 1362
EcoO109I RGGNCCY 1 cut(s) 1831
EcoO65I GGTNACC 1 cut(s) 1362
EcoRI GAATTC 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 478
EcoT14I CCWWGG 2 cut(s) 96, 132
EcoT22I ATGCAT 1 cut(s) 349
ErhI CCWWGG 2 cut(s) 96, 132
FaqI GGGAC 3 cut(s) 564, 707, 1817
FauNDI CATATG 1 cut(s) 1246
FbaI TGATCA 1 cut(s) 354
Fnu4HI GCNGC 3 cut(s) 8, 420, 1572
FokI GGATG 4 cut(s) 531, 1112, 1280, 1957
Fsp4HI GCNGC 3 cut(s) 8, 420, 1572
FspBI CTAG 3 cut(s) 834, 1305, 1986
GlaI GCGC 3 cut(s) 17, 1463, 1984
GluI GCNGC 3 cut(s) 8, 420, 1572
GsaI CCCAGC 2 cut(s) 578, 1740
GsuI CTGGAG 1 cut(s) 579
HaeII RGCGCY 1 cut(s) 1986
HaeIII GGCC 3 cut(s) 83, 94, 907
HapII CCGG 1 cut(s) 32
HhaI GCGC 3 cut(s) 18, 1464, 1985
Hin1I GRCGYC 1 cut(s) 1626
Hin6I GCGC 3 cut(s) 16, 1462, 1983
HinP1I GCGC 3 cut(s) 16, 1462, 1983
HindIII AAGCTT 3 cut(s) 224, 852, 1219
HinfI GANTC 6 cut(s) 124, 728, 1103, 1147, 1380, 1521
HpaII CCGG 1 cut(s) 32
HphI GGTGA 3 cut(s) 1238, 1562, 1934
Hpy166II GTNNAC 5 cut(s) 401, 434, 1076, 1520, 1934
Hpy8I GTNNAC 5 cut(s) 401, 434, 1076, 1520, 1934
Hpy99I CGWCG 2 cut(s) 55, 1808
HpyAV CCTTC 4 cut(s) 457, 593, 1541, 1764
HpyCH4III ACNGT 3 cut(s) 22, 1484, 1798
HpyCH4IV ACGT 6 cut(s) 53, 313, 1323, 1626, 1806, 1930
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 425, 454, 497, 740, 1976
HpySE526I ACGT 6 cut(s) 53, 313, 1323, 1626, 1806, 1930
Hsp92I GRCGYC 1 cut(s) 1626
HspAI GCGC 3 cut(s) 16, 1462, 1983
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 8 cut(s) 85, 354, 979, 1270, 1295, 1301, 1492, 1552
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 4 cut(s) 4, 406, 712, 1276
Lsp1109I GCAGC 2 cut(s) 406, 1583
LweI GCATC 3 cut(s) 406, 1001, 1935
MaeI CTAG 3 cut(s) 834, 1305, 1986
MaeII ACGT 6 cut(s) 53, 313, 1323, 1626, 1806, 1930
MaeIII GTNAC 6 cut(s) 569, 997, 1362, 1484, 1840, 1961
MalI GATC 8 cut(s) 87, 356, 981, 1272, 1297, 1303, 1494, 1554
MbiI CCGCTC 1 cut(s) 7
MboI GATC 8 cut(s) 85, 354, 979, 1270, 1295, 1301, 1492, 1552
MboII GAAGA 9 cut(s) 53, 284, 888, 917, 944, 989, 1131, 1456, 1672
MfeI CAATTG 1 cut(s) 2003
MflI RGATCY 1 cut(s) 979
MhlI GDGCHC 1 cut(s) 1936
MlsI TGGCCA 1 cut(s) 907
MluNI TGGCCA 1 cut(s) 907
MlyI GAGTC 5 cut(s) 118, 722, 1112, 1156, 1515
MmeI TCCRAC 1 cut(s) 1351
Mox20I TGGCCA 1 cut(s) 907
Mph1103I ATGCAT 1 cut(s) 349
MroXI GAANNNNTTC 1 cut(s) 661
MscI TGGCCA 1 cut(s) 907
MseI TTAA 4 cut(s) 171, 539, 1092, 1584
MslI CAYNNNNRTG 2 cut(s) 119, 1004
Msp20I TGGCCA 1 cut(s) 907
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 32, 480
MunI CAATTG 1 cut(s) 2003
MvaI CCWGG 1 cut(s) 480
MvnI CGCG 1 cut(s) 1300
MwoI GCNNNNNNNGC 6 cut(s) 13, 425, 454, 497, 740, 1976
NciI CCSGG 1 cut(s) 32
NdeI CATATG 1 cut(s) 1246
NdeII GATC 8 cut(s) 85, 354, 979, 1270, 1295, 1301, 1492, 1552
NlaIV GGNNCC 2 cut(s) 1775, 1833
NmuCI GTSAC 1 cut(s) 997
NruI TCGCGA 1 cut(s) 1300
NsiI ATGCAT 1 cut(s) 349
NspI RCATGY 3 cut(s) 61, 589, 1082
NspV TTCGAA 2 cut(s) 1172, 1193
OliI CACNNNNGTG 1 cut(s) 1004
PaeR7I CTCGAG 1 cut(s) 1406
PagI TCATGA 1 cut(s) 627
PciI ACATGT 1 cut(s) 1078
PciSI GCTCTTC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 1812
PdmI GAANNNNTTC 1 cut(s) 661
PfeI GAWTC 1 cut(s) 1380
PflMI CCANNNNNTGG 1 cut(s) 1783
PkrI GCNGC 3 cut(s) 9, 421, 1573
PleI GAGTC 5 cut(s) 118, 722, 1111, 1155, 1515
PpsI GAGTC 5 cut(s) 118, 722, 1111, 1155, 1515
Ppu21I YACGTR 1 cut(s) 1931
PpuMI RGGWCCY 1 cut(s) 1831
PscI ACATGT 1 cut(s) 1078
PshAI GACNNNNGTC 1 cut(s) 749
PshBI ATTAAT 2 cut(s) 539, 1092
Psp1406I AACGTT 1 cut(s) 1323
Psp5II RGGWCCY 1 cut(s) 1831
Psp6I CCWGG 1 cut(s) 478
PspEI GGTNACC 1 cut(s) 1362
PspFI CCCAGC 2 cut(s) 574, 1736
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 2 cut(s) 1775, 1833
PspPI GGNCC 3 cut(s) 93, 522, 1831
PspPPI RGGWCCY 1 cut(s) 1831
PsuI RGATCY 1 cut(s) 979
RruI TCGCGA 1 cut(s) 1300
RsaI GTAC 7 cut(s) 56, 584, 769, 1263, 1330, 1422, 1877
RsaNI GTAC 7 cut(s) 55, 583, 768, 1262, 1329, 1421, 1876
RseI CAYNNNNRTG 2 cut(s) 119, 1004
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 4 cut(s) 171, 539, 1092, 1584
SatI GCNGC 3 cut(s) 8, 420, 1572
Sau3AI GATC 8 cut(s) 85, 354, 979, 1270, 1295, 1301, 1492, 1552
Sau96I GGNCC 3 cut(s) 93, 522, 1831
SchI GAGTC 5 cut(s) 118, 722, 1112, 1156, 1515
ScrFI CCNGG 2 cut(s) 32, 480
SduI GDGCHC 1 cut(s) 1936
SfaNI GCATC 3 cut(s) 406, 1001, 1935
SfcI CTRYAG 1 cut(s) 651
Sfr274I CTCGAG 1 cut(s) 1406
SfuI TTCGAA 2 cut(s) 1172, 1193
SinI GGWCC 2 cut(s) 522, 1831
SlaI CTCGAG 1 cut(s) 1406
SmiMI CAYNNNNRTG 2 cut(s) 119, 1004
SmlI CTYRAG 4 cut(s) 1406, 1523, 1680, 1916
SmoI CTYRAG 4 cut(s) 1406, 1523, 1680, 1916
SsiI CCGC 2 cut(s) 7, 1214
SspI AATATT 1 cut(s) 700
SspMI CTAG 3 cut(s) 834, 1305, 1986
StyD4I CCNGG 2 cut(s) 30, 478
StyI CCWWGG 2 cut(s) 96, 132
TaaI ACNGT 3 cut(s) 22, 1484, 1798
TaiI ACGT 6 cut(s) 56, 316, 1326, 1629, 1809, 1933
TaqI TCGA 7 cut(s) 117, 928, 1068, 1172, 1193, 1317, 1407
TatI WGTACW 3 cut(s) 582, 767, 1420
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 1 cut(s) 1380
Tru1I TTAA 4 cut(s) 171, 539, 1092, 1584
Tru9I TTAA 4 cut(s) 171, 539, 1092, 1584
TseFI GTSAC 1 cut(s) 997
TseI GCWGC 2 cut(s) 419, 1571
Tsp45I GTSAC 1 cut(s) 997
TspDTI ATGAA 5 cut(s) 245, 402, 951, 1263, 1773
Van91I CCANNNNNTGG 1 cut(s) 1783
VneI GTGCAC 1 cut(s) 1932
VpaK11BI GGWCC 2 cut(s) 522, 1831
VspI ATTAAT 2 cut(s) 539, 1092
XapI RAATTY 4 cut(s) 331, 389, 1058, 1760
XbaI TCTAGA 1 cut(s) 833
XceI RCATGY 3 cut(s) 61, 589, 1082
XcmI CCANNNNNNNNNTGG 2 cut(s) 1637, 1864
XhoI CTCGAG 1 cut(s) 1406
XmnI GAANNNNTTC 1 cut(s) 661
XspI CTAG 3 cut(s) 834, 1305, 1986
ZraI GACGTC 1 cut(s) 1627
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.