Rroxscaffold_4G00328300

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002805
Physical Location & Seq
Reverse (-)
61201626 .. 61213992
12367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00328300.1

Sequence Viewer

Length: 1779 bp
ATGGAGCGGCACAAGCGGTTGGTGGTGTGCTCCGAGAATGAAGCGCTGGCAGAGTACATGTTGAGTAAGTGGCAGGAAATGGCAGACAAGAAGCCCAAAGGAATCTCTGAAAACATTGAAAAGACTCTTACCAAGGCATACTCCAATGTCTGCAACTCTAAGAATCCTGTTAAAACCCTCAAAGAGTTTTCCCAAATCAAGGGTGTTGGGAAATGGATTTTGAAGCTTATGCAAGGGTATTTTGACACCGGTTCAGGCAGTTCTGCACCAGAAGATGTAACTAAAAAGGGTAAGAGAACCAAAGGAAGCAAACGCTATGTTCCACAAAAGAATTCTGTAGCATATGCTTTACTGATCACACTTTACAGGGGAACTGAAAGTGGGAATGAATTTATGCGTAAACAGGAGCTCATTGATGCAGCTGAAGCAAGTGGACTATCTCGTGTTCCAATTATGCCAGAGAAGGGAAAGGGAATACCGGTACACCTGGGAAATTCTACAAGGGAATGGTATAGTGGTTGGTCCTGCATGAAGACATTGATAACCAAGGGACTGGTTGTGAAATCAAGTTGCCCAGCAAAGTACATGCTTACTCAAGAAGGTAAGGAAGCTGCTCGTGAATGTCTCGTGAGGTCTGGTTTGGCGGATCCTGTAGAAATAATTTCTAATGCCAGGGAGTTGTCTACTGGAGATGTGGACGTTTCACATCAGGAACTTTCCAGTCCGGACTCGGCCACAGATATGACATTGTTATCCTGTAATTCAAGTAGGAATCAAAACTCAATTGATATTCCACAGGAATATCTTGACAAGTTTACACGCATGGGGTATTCTGAGGAACAGATGAACCGAGCTATCAGTGAAGTTTTAGAGACTTCCCATAACAAGGAAATCTCTTCACTATGGCCATCAGTTCTTTGTCGCCTTCGAGAAGATGAGGTTTATGGTTTTCATATGAAGTCTCAAAGCGTAAGGGGAAATCTTCGGGCAACACCATCCACTCATACTCTTGTGAATGGTGATAGAGACTGTGTTAGACTGCTCAACACTTCTAGGGATGGAGGTCGTACACCAAGAATTAATTCTGACAAGGTTGCATCAAAGTCGTTTACGACGGGAGCTCCTTCATCTCCTAGTTGTCCTGTGCCAAAGTCTAGTTTCGCTGGTGTTGAAGCAAGTTCGAAAGCTCTGTCTGTGCCACCTTTAAGCTTTGGGGAGAAATTTGAAGATGCATATGAACTAGTCTTTGTATTGGATGATCGAGAACAATTGGCCGCTCAGATATCACGGTCTAGGAGCATTATCGAGAGTGTTATTTCTGGAAGCAAAATCACAGTAGAGGTTAGGCGGTTACCAGTTGGAGACGGAATTTGGATAGCTCGGCACAAACATCTGAAGAGTGAATATGTACTTGATTTTATTGTTGAGAGGAAAAATGTTGATGATTTGCGCTGTTCAATCAGAGACAACCGTTACAGGGATCAAAAATTGAGACTTTTGCGGTGTGGACTTAAGAAGCTGATATATCTCGTGGAAGGTGATCCAAATTCTAGTGAAGCAGCAGAAAGCATCAAAACAGCCTGCTTTACAACCGAACTTTTGGAGGGCTTTGATGTTCAGAGAACCGGAAACCTGACTGAAACCTTGAGGAAGTATGGTTATCTTACTGAAGCAATTTTTAAATATTACAAAGATCTCTCTGAGGAACAGCATAAGCAAGCTGGAGTCTGTCCTCCTTTGATGAATTTAACAAAAGGTGCCAAGACCTGGACAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

592

Amino Acids

66.23

Weight (kDa)

8.97

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_MUS81 PF21136 108 - 205 5.9e-12 Crossover junction endonuclease MUS81-like, winged helix domain
ERCC4 PF02732 419 - 549 7.1e-23 ERCC4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1757
AccB7I CCANNNNNTGG 1 cut(s) 1767
AccBSI CCGCTC 2 cut(s) 7, 1277
AccI GTMKAC 1 cut(s) 683
AccIII TCCGGA 1 cut(s) 724
AciI CCGC 6 cut(s) 7, 16, 644, 1275, 1348, 1501
AclWI GGATC 4 cut(s) 641, 654, 1488, 1535
AcoI YGGCCR 3 cut(s) 732, 905, 1272
AcsI RAATTY 7 cut(s) 331, 389, 493, 1220, 1368, 1546, 1744
AcuI CTGAAG 3 cut(s) 444, 1415, 1689
AfaI GTAC 5 cut(s) 56, 483, 584, 1069, 1410
AfeI AGCGCT 1 cut(s) 45
AfiI CCNNNNNNNGG 5 cut(s) 199, 464, 886, 1477, 1767
AflII CTTAAG 1 cut(s) 1511
AflIII ACRYGT 1 cut(s) 57
AgeI ACCGGT 2 cut(s) 248, 478
AgsI TTSAA 6 cut(s) 119, 223, 765, 1172, 1226, 1458
AhlI ACTAGT 1 cut(s) 1240
AjnI CCWGG 3 cut(s) 486, 671, 1766
AjuI GAANNNNNNNTTGG 2 cut(s) 1066, 1098
Alw21I GWGCWC 3 cut(s) 32, 411, 1123
Alw26I GTCTC 7 cut(s) 629, 866, 966, 1020, 1356, 1458, 1486
AlwI GGATC 4 cut(s) 641, 654, 1488, 1535
Aor13HI TCCGGA 1 cut(s) 724
Aor51HI AGCGCT 1 cut(s) 45
AoxI GGCC 3 cut(s) 732, 905, 1272
ApeKI GCWGC 3 cut(s) 419, 611, 1559
ApoI RAATTY 7 cut(s) 331, 389, 493, 1220, 1368, 1546, 1744
ArsI GACNNNNNNTTYG 2 cut(s) 1175, 1207
AseI ATTAAT 1 cut(s) 1080
AsiGI ACCGGT 2 cut(s) 248, 478
Asp700I GAANNNNTTC 1 cut(s) 1081
AspLEI GCGC 2 cut(s) 46, 1452
AspS9I GGNCC 1 cut(s) 522
AsuHPI GGTGA 2 cut(s) 1031, 1550
AsuII TTCGAA 1 cut(s) 1181
AvaII GGWCC 1 cut(s) 522
BalI TGGCCA 1 cut(s) 907
BamHI GGATCC 1 cut(s) 646
BanI GGYRCC 1 cut(s) 1757
BanII GRGCYC 2 cut(s) 411, 1123
BauI CACGAG 4 cut(s) 441, 615, 626, 1529
BbsI GAAGAC 1 cut(s) 539
Bbv12I GWGCWC 3 cut(s) 32, 411, 1123
BbvI GCAGC 3 cut(s) 431, 598, 1571
BccI CCATC 3 cut(s) 916, 1003, 1052
BcgI CGANNNNNNTGC 2 cut(s) 1086, 1120
BciT130I CCWGG 3 cut(s) 488, 673, 1768
BclI TGATCA 1 cut(s) 354
BcoDI GTCTC 7 cut(s) 629, 866, 966, 1020, 1356, 1458, 1486
BcuI ACTAGT 1 cut(s) 1240
BfaI CTAG 6 cut(s) 1053, 1134, 1155, 1241, 1293, 1551
BfmI CTRYAG 2 cut(s) 336, 651
BfoI RGCGCY 1 cut(s) 47
BfrI CTTAAG 1 cut(s) 1511
BglII AGATCT 1 cut(s) 1693
BisI GCNGC 5 cut(s) 8, 420, 612, 1275, 1560
BlsI GCNGC 5 cut(s) 9, 421, 613, 1276, 1561
Bme1390I CCNGG 3 cut(s) 488, 673, 1768
Bme18I GGWCC 1 cut(s) 522
BmgT120I GGNCC 1 cut(s) 522
BmiI GGNNCC 2 cut(s) 648, 1759
BmrFI CCNGG 3 cut(s) 488, 673, 1768
BmsI GCATC 4 cut(s) 406, 1106, 1219, 1578
BpiI GAAGAC 1 cut(s) 539
BpmI CTGGAG 2 cut(s) 708, 1743
Bpu14I TTCGAA 1 cut(s) 1181
BpuEI CTTGAG 2 cut(s) 579, 1666
BsaJI CCNNGG 4 cut(s) 132, 487, 546, 672
BsaWI WCCGGW 4 cut(s) 248, 478, 724, 1625
BsaXI ACNNNNNCTCC 2 cut(s) 1105, 1135
Bsc4I CCNNNNNNNGG 5 cut(s) 199, 464, 886, 1477, 1767
Bse118I RCCGGY 2 cut(s) 248, 478
Bse1I ACTGG 4 cut(s) 558, 691, 720, 1355
BseAI TCCGGA 1 cut(s) 724
BseBI CCWGG 3 cut(s) 488, 673, 1768
BseDI CCNNGG 4 cut(s) 132, 487, 546, 672
BseGI GGATG 3 cut(s) 995, 1063, 1261
BseLI CCNNNNNNNGG 5 cut(s) 199, 464, 886, 1477, 1767
BseMII CTCAG 3 cut(s) 825, 1292, 1692
BseNI ACTGG 4 cut(s) 558, 691, 720, 1355
BseXI GCAGC 3 cut(s) 431, 598, 1571
BseYI CCCAGC 1 cut(s) 574
BsgI GTGCAG 1 cut(s) 249
BshFI GGCC 3 cut(s) 734, 907, 1274
BshNI GGYRCC 1 cut(s) 1757
BshTI ACCGGT 2 cut(s) 248, 478
BsiHKAI GWGCWC 3 cut(s) 32, 411, 1123
BsiSI CCGG 4 cut(s) 249, 479, 725, 1626
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 5 cut(s) 199, 464, 886, 1477, 1767
BsmAI GTCTC 7 cut(s) 629, 866, 966, 1020, 1356, 1458, 1486
BsmBI CGTCTC 1 cut(s) 1356
BsmFI GGGAC 1 cut(s) 564
BsnI GGCC 3 cut(s) 734, 907, 1274
Bsp119I TTCGAA 1 cut(s) 1181
Bsp1286I GDGCHC 3 cut(s) 32, 411, 1123
Bsp13I TCCGGA 1 cut(s) 724
Bsp143I GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
BspACI CCGC 6 cut(s) 7, 16, 644, 1275, 1348, 1501
BspANI GGCC 3 cut(s) 734, 907, 1274
BspCNI CTCAG 3 cut(s) 826, 1291, 1693
BspEI TCCGGA 1 cut(s) 724
BspLI GGNNCC 2 cut(s) 648, 1759
BspPI GGATC 4 cut(s) 641, 654, 1488, 1535
BspT104I TTCGAA 1 cut(s) 1181
BspT107I GGYRCC 1 cut(s) 1757
BspTI CTTAAG 1 cut(s) 1511
BsrBI CCGCTC 2 cut(s) 7, 1277
BsrFI RCCGGY 2 cut(s) 248, 478
BsrI ACTGG 4 cut(s) 558, 691, 720, 1355
BssAI RCCGGY 2 cut(s) 248, 478
BssECI CCNNGG 4 cut(s) 132, 487, 546, 672
BssMI GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
BssSI CACGAG 4 cut(s) 441, 615, 626, 1529
BssT1I CCWWGG 2 cut(s) 132, 546
Bst2BI CACGAG 4 cut(s) 441, 615, 626, 1529
Bst2UI CCWGG 3 cut(s) 488, 673, 1768
Bst4CI ACNGT 4 cut(s) 1031, 1290, 1336, 1472
Bst6I CTCTTC 2 cut(s) 901, 1391
BstAFI CTTAAG 1 cut(s) 1511
BstBI TTCGAA 1 cut(s) 1181
BstC8I GCNNGC 3 cut(s) 48, 1582, 1719
BstDEI CTNAG 4 cut(s) 159, 834, 1278, 1701
BstEII GGTNACC 1 cut(s) 1350
BstF5I GGATG 3 cut(s) 995, 1063, 1261
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 2 cut(s) 46, 1452
BstKTI GATC 6 cut(s) 357, 649, 1261, 1483, 1543, 1696
BstMAI GTCTC 7 cut(s) 629, 866, 966, 1020, 1356, 1458, 1486
BstMBI GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
BstMWI GCNNNNNNNGC 2 cut(s) 13, 425
BstNI CCWGG 3 cut(s) 488, 673, 1768
BstNSI RCATGY 2 cut(s) 61, 589
BstPI GGTNACC 1 cut(s) 1350
BstSCI CCNGG 3 cut(s) 486, 671, 1766
BstSFI CTRYAG 2 cut(s) 336, 651
BstV1I GCAGC 3 cut(s) 431, 598, 1571
BstV2I GAAGAC 1 cut(s) 539
BstX2I RGATCY 2 cut(s) 646, 1693
BstXI CCANNNNNNTGG 1 cut(s) 553
BstYI RGATCY 2 cut(s) 646, 1693
BsuRI GGCC 3 cut(s) 734, 907, 1274
BtsCI GGATG 3 cut(s) 995, 1063, 1261
BtsIMutI CAGTG 1 cut(s) 865
Cac8I GCNNGC 3 cut(s) 48, 1582, 1719
CfoI GCGC 2 cut(s) 46, 1452
Cfr10I RCCGGY 2 cut(s) 248, 478
Cfr13I GGNCC 1 cut(s) 522
Csp6I GTAC 5 cut(s) 55, 482, 583, 1068, 1409
CspAI ACCGGT 2 cut(s) 248, 478
CviAII CATG 4 cut(s) 58, 529, 586, 823
CviQI GTAC 5 cut(s) 55, 482, 583, 1068, 1409
DdeI CTNAG 4 cut(s) 159, 834, 1278, 1701
DpnI GATC 6 cut(s) 356, 648, 1260, 1482, 1542, 1695
DpnII GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
DraI TTTAAA 1 cut(s) 1681
EaeI YGGCCR 3 cut(s) 732, 905, 1272
Eam1104I CTCTTC 2 cut(s) 901, 1391
EarI CTCTTC 2 cut(s) 901, 1391
EciI GGCGGA 1 cut(s) 659
Ecl136II GAGCTC 2 cut(s) 409, 1121
Eco130I CCWWGG 2 cut(s) 132, 546
Eco24I GRGCYC 2 cut(s) 411, 1123
Eco32I GATATC 1 cut(s) 1284
Eco47I GGWCC 1 cut(s) 522
Eco47III AGCGCT 1 cut(s) 45
Eco53kI GAGCTC 2 cut(s) 409, 1121
Eco57I CTGAAG 3 cut(s) 444, 1415, 1689
Eco91I GGTNACC 1 cut(s) 1350
EcoICRI GAGCTC 2 cut(s) 409, 1121
EcoO65I GGTNACC 1 cut(s) 1350
EcoRI GAATTC 1 cut(s) 331
EcoRII CCWGG 3 cut(s) 486, 671, 1766
EcoRV GATATC 1 cut(s) 1284
EcoT14I CCWWGG 2 cut(s) 132, 546
EcoT22I ATGCAT 1 cut(s) 1234
EcoT38I GRGCYC 2 cut(s) 411, 1123
ErhI CCWWGG 2 cut(s) 132, 546
Esp3I CGTCTC 1 cut(s) 1356
FaeI CATG 4 cut(s) 61, 532, 589, 826
FaqI GGGAC 1 cut(s) 564
FatI CATG 4 cut(s) 57, 528, 585, 822
FauNDI CATATG 3 cut(s) 343, 954, 1234
FbaI TGATCA 1 cut(s) 354
FblI GTMKAC 1 cut(s) 683
Fnu4HI GCNGC 5 cut(s) 8, 420, 612, 1275, 1560
FokI GGATG 3 cut(s) 982, 1070, 1268
FriOI GRGCYC 2 cut(s) 411, 1123
Fsp4HI GCNGC 5 cut(s) 8, 420, 612, 1275, 1560
FspBI CTAG 6 cut(s) 1053, 1134, 1155, 1241, 1293, 1551
GlaI GCGC 2 cut(s) 45, 1451
GluI GCNGC 5 cut(s) 8, 420, 612, 1275, 1560
GsaI CCCAGC 1 cut(s) 578
GsuI CTGGAG 2 cut(s) 708, 1743
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 3 cut(s) 734, 907, 1274
HapII CCGG 4 cut(s) 249, 479, 725, 1626
HhaI GCGC 2 cut(s) 46, 1452
Hin1II CATG 4 cut(s) 61, 532, 589, 826
Hin6I GCGC 2 cut(s) 44, 1450
HinP1I GCGC 2 cut(s) 44, 1450
HindIII AAGCTT 2 cut(s) 224, 1207
HinfI GANTC 6 cut(s) 102, 124, 163, 728, 772, 1725
HpaII CCGG 4 cut(s) 249, 479, 725, 1626
HphI GGTGA 2 cut(s) 1031, 1550
Hpy166II GTNNAC 9 cut(s) 401, 434, 484, 684, 697, 816, 1070, 1110, 1508
Hpy188I TCNGA 9 cut(s) 34, 109, 835, 1087, 1281, 1395, 1463, 1620, 1702
Hpy8I GTNNAC 9 cut(s) 401, 434, 484, 684, 697, 816, 1070, 1110, 1508
Hpy99I CGWCG 1 cut(s) 1117
HpyAV CCTTC 5 cut(s) 457, 593, 935, 1134, 1529
HpyCH4III ACNGT 4 cut(s) 1031, 1290, 1336, 1472
HpyCH4IV ACGT 1 cut(s) 699
HpyCH4V TGCA 7 cut(s) 153, 232, 266, 419, 528, 1097, 1232
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 425
HpyF3I CTNAG 4 cut(s) 159, 834, 1278, 1701
HpySE526I ACGT 1 cut(s) 699
Hsp92II CATG 4 cut(s) 61, 532, 589, 826
HspAI GCGC 2 cut(s) 44, 1450
Kpn2I TCCGGA 1 cut(s) 724
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
LmnI GCTCC 6 cut(s) 4, 35, 406, 1118, 1126, 1296
Lsp1109I GCAGC 3 cut(s) 431, 598, 1571
LweI GCATC 4 cut(s) 406, 1106, 1219, 1578
MaeI CTAG 6 cut(s) 1053, 1134, 1155, 1241, 1293, 1551
MaeII ACGT 1 cut(s) 699
MaeIII GTNAC 3 cut(s) 277, 1350, 1472
MalI GATC 6 cut(s) 356, 648, 1260, 1482, 1542, 1695
MbiI CCGCTC 2 cut(s) 7, 1277
MboI GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
MboII GAAGA 7 cut(s) 284, 544, 888, 944, 974, 1238, 1408
MfeI CAATTG 2 cut(s) 783, 1268
MflI RGATCY 2 cut(s) 646, 1693
MhlI GDGCHC 3 cut(s) 32, 411, 1123
MlsI TGGCCA 1 cut(s) 907
MluNI TGGCCA 1 cut(s) 907
MlyI GAGTC 3 cut(s) 118, 722, 1734
MmeI TCCRAC 1 cut(s) 1339
Mox20I TGGCCA 1 cut(s) 907
Mph1103I ATGCAT 1 cut(s) 1234
MroI TCCGGA 1 cut(s) 724
MroXI GAANNNNTTC 1 cut(s) 1081
MscI TGGCCA 1 cut(s) 907
MseI TTAA 6 cut(s) 171, 1080, 1205, 1512, 1680, 1748
MslI CAYNNNNRTG 1 cut(s) 740
Msp20I TGGCCA 1 cut(s) 907
MspA1I CMGCKG 1 cut(s) 422
MspCI CTTAAG 1 cut(s) 1511
MspI CCGG 4 cut(s) 249, 479, 725, 1626
MspR9I CCNGG 3 cut(s) 488, 673, 1768
MunI CAATTG 2 cut(s) 783, 1268
MvaI CCWGG 3 cut(s) 488, 673, 1768
MwoI GCNNNNNNNGC 2 cut(s) 13, 425
NdeI CATATG 3 cut(s) 343, 954, 1234
NdeII GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
NlaIII CATG 4 cut(s) 61, 532, 589, 826
NlaIV GGNNCC 2 cut(s) 648, 1759
NmeAIII GCCGAG 2 cut(s) 710, 1360
NsiI ATGCAT 1 cut(s) 1234
NspI RCATGY 2 cut(s) 61, 589
NspV TTCGAA 1 cut(s) 1181
PciI ACATGT 1 cut(s) 57
PdmI GAANNNNTTC 1 cut(s) 1081
PfeI GAWTC 3 cut(s) 102, 163, 772
PflMI CCANNNNNTGG 1 cut(s) 1767
PinAI ACCGGT 2 cut(s) 248, 478
PkrI GCNGC 5 cut(s) 9, 421, 613, 1276, 1561
PleI GAGTC 3 cut(s) 118, 722, 1733
PpsI GAGTC 3 cut(s) 118, 722, 1733
PscI ACATGT 1 cut(s) 57
PshBI ATTAAT 1 cut(s) 1080
Psp124BI GAGCTC 2 cut(s) 411, 1123
Psp6I CCWGG 3 cut(s) 486, 671, 1766
PspEI GGTNACC 1 cut(s) 1350
PspFI CCCAGC 1 cut(s) 574
PspGI CCWGG 3 cut(s) 486, 671, 1766
PspN4I GGNNCC 2 cut(s) 648, 1759
PspPI GGNCC 1 cut(s) 522
PsuI RGATCY 2 cut(s) 646, 1693
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 5 cut(s) 56, 483, 584, 1069, 1410
RsaNI GTAC 5 cut(s) 55, 482, 583, 1068, 1409
RseI CAYNNNNRTG 1 cut(s) 740
SacI GAGCTC 2 cut(s) 411, 1123
SaqAI TTAA 6 cut(s) 171, 1080, 1205, 1512, 1680, 1748
SatI GCNGC 5 cut(s) 8, 420, 612, 1275, 1560
Sau3AI GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
Sau96I GGNCC 1 cut(s) 522
SchI GAGTC 3 cut(s) 118, 722, 1734
ScrFI CCNGG 3 cut(s) 488, 673, 1768
SduI GDGCHC 3 cut(s) 32, 411, 1123
SfaNI GCATC 4 cut(s) 406, 1106, 1219, 1578
SfcI CTRYAG 2 cut(s) 336, 651
SfuI TTCGAA 1 cut(s) 1181
SinI GGWCC 1 cut(s) 522
SmiMI CAYNNNNRTG 1 cut(s) 740
SmlI CTYRAG 3 cut(s) 594, 1511, 1645
SmoI CTYRAG 3 cut(s) 594, 1511, 1645
SpeI ACTAGT 1 cut(s) 1240
SsiI CCGC 6 cut(s) 7, 16, 644, 1275, 1348, 1501
SspI AATATT 1 cut(s) 1685
SspMI CTAG 6 cut(s) 1053, 1134, 1155, 1241, 1293, 1551
SstI GAGCTC 2 cut(s) 411, 1123
StyD4I CCNGG 3 cut(s) 486, 671, 1766
StyI CCWWGG 2 cut(s) 132, 546
TaaI ACNGT 4 cut(s) 1031, 1290, 1336, 1472
TaiI ACGT 1 cut(s) 702
TaqI TCGA 4 cut(s) 928, 1181, 1261, 1305
TatI WGTACW 3 cut(s) 54, 582, 1408
TauI GCSGC 2 cut(s) 10, 1277
TfiI GAWTC 3 cut(s) 102, 163, 772
Tru1I TTAA 6 cut(s) 171, 1080, 1205, 1512, 1680, 1748
Tru9I TTAA 6 cut(s) 171, 1080, 1205, 1512, 1680, 1748
TscAI CASTG 1 cut(s) 865
TseI GCWGC 3 cut(s) 419, 611, 1559
TspDTI ATGAA 9 cut(s) 54, 402, 545, 860, 941, 971, 1116, 1251, 1757
TspGWI ACGGA 1 cut(s) 1380
TspRI CASTG 1 cut(s) 865
Van91I CCANNNNNTGG 1 cut(s) 1767
Vha464I CTTAAG 1 cut(s) 1511
VpaK11BI GGWCC 1 cut(s) 522
VspI ATTAAT 1 cut(s) 1080
XapI RAATTY 7 cut(s) 331, 389, 493, 1220, 1368, 1546, 1744
XceI RCATGY 2 cut(s) 61, 589
XmiI GTMKAC 1 cut(s) 683
XmnI GAANNNNTTC 1 cut(s) 1081
XspI CTAG 6 cut(s) 1053, 1134, 1155, 1241, 1293, 1551
Zsp2I ATGCAT 1 cut(s) 1234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.