Rh3AG289900

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002805
Physical Location & Seq
Forward (+)
34796653 .. 34803938
7286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG289900.1

Sequence Viewer

Length: 1344 bp
ATGAGGGATCCACTGGCGTTTCGTACCTCGAGCGGATCAGCCAAGAGAACCAAATCAACCAAACGGTACGTTCCGCAGAAGAATTCCGTGGCATATGCTTTGTTGATCACGCTTTACAGGGGAACCGAAAATGGAGATGAATTTATGCGTAAACAGGAGCTTATTGATGCAGCTGAAGCAAGTGGACTTTCTCGTGTGCCGATTATGCCGGAGAAGGGGAAGGGAAAGCCTACAACTCTCGGCAATTCTGCCAAGGAATGGTATAGTGGTTGGTCCTGCATGAAGACATTGATAACCAAAGGACTGGTTCTGAAATCAAGTTGCCCCGCAAAGTACATGCTTACTGAAGAAGGTCAGGAAGTTGCCCGTGAATGTCTCATGAGGTCTGGTTTGGCGGATCCTGTGGAAAAAGTTTCTAATGCCAAAGGGTTGTCTGTCGTGGATGTGAATGTATCAGAACAGGACCTTTCTCATCCTGAGTCAGTGACAGATATGACATTGTTGTCCAGTAATTCGAGTAGTGAGGAGAACTTAATTGATATGCCATGGGAATGTCTTGACAAGAGTTGTCCCATGCCAAAGTCTAGTTCTCCGGGTGTCAAAGCAAGTTCAAAAGCTTTATCCATTCCACCTCTAAGCTTTGGGGAGAGATTTGAAGATGCTTATGAAGTAGTCTTTCTATTAGATGACCGAGAGCAATTGGCCACTCAGATATTGCGGTCTAGGAGCATTATTGAGAGTGTTATTTCTGAATCCAAAATTAAAGTAGAGGTCAGACGGTTACCAGTTGGAGATGGGATTTGGATAGCTCGCCATAATTATCTTGAGACTGAATATGTACTTGATTTTATTGTTGAGAGGAAGAATGTTGATGATTTACACTGTTCAATAAGAGACAACCGTTACAGGGATCAAAAATTGAGACTTTTGAGGTGCGGACTCAAAAAGCTGATATATCTTGTGGAAGGTGATCCCAATTCTTGTGCAGCAGCAGAAAGCATTAAAACAGCCTGCTTCACAACTGAAATTTTGGAGGGTTTTGATGTTCAGAGAACTGAAAACTTGACTGAAACCTTAAGGAAATATGGTTATCTTACTGAAGCAATATTTAAATATTACAAAGAACTTCCTGAGGAACAGCGTAAGCATGCTGGAGTTTGTCTTACATTTGATGAATTTAACAAAAGGAGTCAAGACCTGGATAAAATGACAGTCAGTGACGTATTTTCTGTTCAGCTCATGCAGGTCCCCCAGGTGACTGAGGAAGTTGCCATTGCTGTTCTGGATTTGTACCCAACGCCTTTTTCTCTTGCCCATGAATACTCTCTACTTGTAAGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

447

Amino Acids

50.29

Weight (kDa)

5.58

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_MUS81 PF21136 25 - 123 2e-13 Crossover junction endonuclease MUS81-like, winged helix domain
ERCC4 PF02732 229 - 359 2.7e-24 ERCC4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 502
Acc36I ACCTGC 1 cut(s) 1234
AccB7I CCANNNNNTGG 1 cut(s) 258
AccBSI CCGCTC 1 cut(s) 33
AciI CCGC 6 cut(s) 33, 74, 327, 395, 718, 936
AclWI GGATC 7 cut(s) 2, 15, 43, 392, 405, 918, 965
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 4 cut(s) 82, 140, 1026, 1175
AcuI CTGAAG 3 cut(s) 195, 366, 1119
AfaI GTAC 5 cut(s) 25, 68, 335, 840, 1292
AfiI CCNNNNNNNGG 3 cut(s) 215, 258, 907
AflII CTTAAG 1 cut(s) 1075
AgsI TTSAA 3 cut(s) 612, 656, 888
AjnI CCWGG 2 cut(s) 1197, 1251
AjuI GAANNNNNNNTTGG 4 cut(s) 571, 603, 1288, 1320
AluBI AGCT 7 cut(s) 160, 173, 617, 639, 809, 949, 1237
AluI AGCT 7 cut(s) 160, 173, 617, 639, 809, 949, 1237
Alw26I GTCTC 4 cut(s) 380, 821, 888, 916
AlwI GGATC 7 cut(s) 2, 15, 43, 392, 405, 918, 965
Ama87I CYCGRG 1 cut(s) 28
AoxI GGCC 1 cut(s) 702
ApeKI GCWGC 3 cut(s) 170, 986, 989
ApoI RAATTY 4 cut(s) 82, 140, 1026, 1175
AspS9I GGNCC 3 cut(s) 273, 463, 1246
AsuC2I CCSGG 1 cut(s) 594
AsuHPI GGTGA 2 cut(s) 980, 1267
AvaI CYCGRG 1 cut(s) 28
AvaII GGWCC 3 cut(s) 273, 463, 1246
AxyI CCTNAGG 1 cut(s) 1131
BalI TGGCCA 1 cut(s) 704
BamHI GGATCC 2 cut(s) 7, 397
BauI CACGAG 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 290
BbvI GCAGC 3 cut(s) 182, 998, 1001
BccI CCATC 1 cut(s) 788
BciT130I CCWGG 2 cut(s) 1199, 1253
BclI TGATCA 1 cut(s) 105
BcnI CCSGG 1 cut(s) 594
BcoDI GTCTC 4 cut(s) 380, 821, 888, 916
BfaI CTAG 2 cut(s) 585, 723
BfrI CTTAAG 1 cut(s) 1075
BfuAI ACCTGC 1 cut(s) 1234
BisI GCNGC 3 cut(s) 171, 987, 990
BlsI GCNGC 3 cut(s) 172, 988, 991
Bme1390I CCNGG 3 cut(s) 594, 1199, 1253
Bme18I GGWCC 3 cut(s) 273, 463, 1246
BmeT110I CYCGRG 1 cut(s) 28
BmgT120I GGNCC 3 cut(s) 273, 463, 1246
BmiI GGNNCC 4 cut(s) 9, 124, 399, 1248
BmrFI CCNGG 3 cut(s) 594, 1199, 1253
BmsI GCATC 2 cut(s) 157, 649
BpiI GAAGAC 1 cut(s) 290
BpmI CTGGAG 1 cut(s) 1173
BpuEI CTTGAG 1 cut(s) 845
BpuMI CCSGG 1 cut(s) 594
BsaJI CCNNGG 4 cut(s) 87, 252, 545, 1251
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 258, 907
Bse1I ACTGG 4 cut(s) 18, 309, 507, 785
Bse21I CCTNAGG 1 cut(s) 1131
Bse3DI GCAATG 1 cut(s) 1272
BseBI CCWGG 2 cut(s) 1199, 1253
BseDI CCNNGG 4 cut(s) 87, 252, 545, 1251
BseGI GGATG 2 cut(s) 448, 472
BseLI CCNNNNNNNGG 3 cut(s) 215, 258, 907
BseMI GCAATG 1 cut(s) 1272
BseMII CTCAG 4 cut(s) 468, 722, 1122, 1251
BseNI ACTGG 4 cut(s) 18, 309, 507, 785
BseRI GAGGAG 1 cut(s) 539
BseXI GCAGC 3 cut(s) 182, 998, 1001
BsgI GTGCAG 1 cut(s) 1005
BshFI GGCC 1 cut(s) 704
BsiHKCI CYCGRG 1 cut(s) 28
BsiSI CCGG 2 cut(s) 209, 593
BslFI GGGAC 2 cut(s) 555, 1232
BslI CCNNNNNNNGG 3 cut(s) 215, 258, 907
BsmAI GTCTC 4 cut(s) 380, 821, 888, 916
BsmFI GGGAC 2 cut(s) 555, 1232
BsnI GGCC 1 cut(s) 704
BsoBI CYCGRG 1 cut(s) 28
Bsp143I GATC 6 cut(s) 7, 35, 105, 397, 910, 970
Bsp19I CCATGG 1 cut(s) 545
BspACI CCGC 6 cut(s) 33, 74, 327, 395, 718, 936
BspANI GGCC 1 cut(s) 704
BspCNI CTCAG 4 cut(s) 469, 721, 1123, 1252
BspHI TCATGA 1 cut(s) 378
BspLI GGNNCC 4 cut(s) 9, 124, 399, 1248
BspMI ACCTGC 1 cut(s) 1234
BspPI GGATC 7 cut(s) 2, 15, 43, 392, 405, 918, 965
BspTI CTTAAG 1 cut(s) 1075
BsrBI CCGCTC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 1272
BsrI ACTGG 4 cut(s) 18, 309, 507, 785
BssECI CCNNGG 4 cut(s) 87, 252, 545, 1251
BssMI GATC 6 cut(s) 7, 35, 105, 397, 910, 970
BssSI CACGAG 1 cut(s) 192
BssT1I CCWWGG 2 cut(s) 252, 545
Bst2BI CACGAG 1 cut(s) 192
Bst2UI CCWGG 2 cut(s) 1199, 1253
Bst4CI ACNGT 5 cut(s) 66, 780, 884, 902, 1213
BstAFI CTTAAG 1 cut(s) 1075
BstC8I GCNNGC 3 cut(s) 811, 1012, 1149
BstDEI CTNAG 6 cut(s) 477, 635, 708, 1131, 1260, 1341
BstDSI CCRYGG 2 cut(s) 87, 545
BstEII GGTNACC 1 cut(s) 780
BstF5I GGATG 2 cut(s) 448, 472
BstKTI GATC 6 cut(s) 10, 38, 108, 400, 913, 973
BstMAI GTCTC 4 cut(s) 380, 821, 888, 916
BstMBI GATC 6 cut(s) 7, 35, 105, 397, 910, 970
BstMWI GCNNNNNNNGC 2 cut(s) 176, 205
BstNI CCWGG 2 cut(s) 1199, 1253
BstNSI RCATGY 2 cut(s) 340, 1151
BstPI GGTNACC 1 cut(s) 780
BstSCI CCNGG 3 cut(s) 592, 1197, 1251
BstV1I GCAGC 3 cut(s) 182, 998, 1001
BstV2I GAAGAC 1 cut(s) 290
BstX2I RGATCY 2 cut(s) 7, 397
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 2 cut(s) 7, 397
Bsu36I CCTNAGG 1 cut(s) 1131
BsuRI GGCC 1 cut(s) 704
BtgI CCRYGG 2 cut(s) 87, 545
BtsCI GGATG 2 cut(s) 448, 472
BtsIMutI CAGTG 4 cut(s) 11, 489, 880, 1222
BveI ACCTGC 1 cut(s) 1234
Cac8I GCNNGC 3 cut(s) 811, 1012, 1149
CciI TCATGA 1 cut(s) 378
Cfr13I GGNCC 3 cut(s) 273, 463, 1246
Csp6I GTAC 5 cut(s) 24, 67, 334, 839, 1291
CviAII CATG 8 cut(s) 280, 337, 379, 546, 574, 1148, 1240, 1316
CviQI GTAC 5 cut(s) 24, 67, 334, 839, 1291
DdeI CTNAG 6 cut(s) 477, 635, 708, 1131, 1260, 1341
DpnI GATC 6 cut(s) 9, 37, 107, 399, 912, 972
DpnII GATC 6 cut(s) 7, 35, 105, 397, 910, 970
DraI TTTAAA 1 cut(s) 1111
DrdI GACNNNNNNGTC 1 cut(s) 502
DseDI GACNNNNNNGTC 1 cut(s) 502
EaeI YGGCCR 1 cut(s) 702
EciI GGCGGA 1 cut(s) 410
Eco130I CCWWGG 2 cut(s) 252, 545
Eco47I GGWCC 3 cut(s) 273, 463, 1246
Eco57I CTGAAG 3 cut(s) 195, 366, 1119
Eco81I CCTNAGG 1 cut(s) 1131
Eco88I CYCGRG 1 cut(s) 28
Eco91I GGTNACC 1 cut(s) 780
EcoO109I RGGNCCY 2 cut(s) 463, 1246
EcoO65I GGTNACC 1 cut(s) 780
EcoRI GAATTC 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 1197, 1251
EcoT14I CCWWGG 2 cut(s) 252, 545
ErhI CCWWGG 2 cut(s) 252, 545
FaeI CATG 8 cut(s) 283, 340, 382, 549, 577, 1151, 1243, 1319
FaqI GGGAC 2 cut(s) 555, 1232
FatI CATG 8 cut(s) 279, 336, 378, 545, 573, 1147, 1239, 1315
FauI CCCGC 1 cut(s) 334
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 1 cut(s) 105
Fnu4HI GCNGC 3 cut(s) 171, 987, 990
FokI GGATG 2 cut(s) 455, 459
Fsp4HI GCNGC 3 cut(s) 171, 987, 990
FspBI CTAG 2 cut(s) 585, 723
GluI GCNGC 3 cut(s) 171, 987, 990
GsuI CTGGAG 1 cut(s) 1173
HaeIII GGCC 1 cut(s) 704
HapII CCGG 2 cut(s) 209, 593
Hin1II CATG 8 cut(s) 283, 340, 382, 549, 577, 1151, 1243, 1319
HindIII AAGCTT 2 cut(s) 615, 637
HinfI GANTC 4 cut(s) 479, 752, 939, 1189
HpaII CCGG 2 cut(s) 209, 593
HphI GGTGA 2 cut(s) 980, 1267
Hpy166II GTNNAC 2 cut(s) 152, 185
Hpy188I TCNGA 6 cut(s) 312, 457, 711, 751, 776, 1050
Hpy188III TCNNGA 8 cut(s) 356, 379, 476, 557, 824, 1130, 1193, 1283
Hpy8I GTNNAC 2 cut(s) 152, 185
HpyAV CCTTC 4 cut(s) 208, 214, 344, 959
HpyCH4III ACNGT 5 cut(s) 66, 780, 884, 902, 1213
HpyCH4IV ACGT 2 cut(s) 69, 1221
HpyCH4V TGCA 4 cut(s) 170, 279, 986, 1243
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 205
HpyF3I CTNAG 6 cut(s) 477, 635, 708, 1131, 1260, 1341
HpySE526I ACGT 2 cut(s) 69, 1221
Hsp92II CATG 8 cut(s) 283, 340, 382, 549, 577, 1151, 1243, 1319
Ksp22I TGATCA 1 cut(s) 105
Kzo9I GATC 6 cut(s) 7, 35, 105, 397, 910, 970
LmnI GCTCC 2 cut(s) 157, 726
Lsp1109I GCAGC 3 cut(s) 182, 998, 1001
LweI GCATC 2 cut(s) 157, 649
MaeI CTAG 2 cut(s) 585, 723
MaeII ACGT 2 cut(s) 69, 1221
MaeIII GTNAC 5 cut(s) 484, 780, 902, 1217, 1255
MalI GATC 6 cut(s) 9, 37, 107, 399, 912, 972
MbiI CCGCTC 1 cut(s) 33
MboI GATC 6 cut(s) 7, 35, 105, 397, 910, 970
MboII GAAGA 5 cut(s) 91, 295, 359, 668, 874
MfeI CAATTG 1 cut(s) 698
MflI RGATCY 2 cut(s) 7, 397
MlsI TGGCCA 1 cut(s) 704
MluNI TGGCCA 1 cut(s) 704
MlyI GAGTC 3 cut(s) 488, 933, 1198
MmeI TCCRAC 1 cut(s) 769
Mox20I TGGCCA 1 cut(s) 704
MscI TGGCCA 1 cut(s) 704
MseI TTAA 6 cut(s) 533, 762, 1002, 1076, 1110, 1179
MslI CAYNNNNRTG 1 cut(s) 550
Msp20I TGGCCA 1 cut(s) 704
MspA1I CMGCKG 1 cut(s) 173
MspCI CTTAAG 1 cut(s) 1075
MspI CCGG 2 cut(s) 209, 593
MspR9I CCNGG 3 cut(s) 594, 1199, 1253
MunI CAATTG 1 cut(s) 698
MvaI CCWGG 2 cut(s) 1199, 1253
MwoI GCNNNNNNNGC 2 cut(s) 176, 205
NciI CCSGG 1 cut(s) 594
NcoI CCATGG 1 cut(s) 545
NdeI CATATG 1 cut(s) 94
NdeII GATC 6 cut(s) 7, 35, 105, 397, 910, 970
NlaIII CATG 8 cut(s) 283, 340, 382, 549, 577, 1151, 1243, 1319
NlaIV GGNNCC 4 cut(s) 9, 124, 399, 1248
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 3 cut(s) 484, 1217, 1255
NspI RCATGY 2 cut(s) 340, 1151
PaeI GCATGC 1 cut(s) 1151
PaeR7I CTCGAG 1 cut(s) 28
PagI TCATGA 1 cut(s) 378
PfeI GAWTC 1 cut(s) 752
PflMI CCANNNNNTGG 1 cut(s) 258
PkrI GCNGC 3 cut(s) 172, 988, 991
PleI GAGTC 3 cut(s) 487, 933, 1197
PpsI GAGTC 3 cut(s) 487, 933, 1197
PpuMI RGGWCCY 2 cut(s) 463, 1246
Psp5II RGGWCCY 2 cut(s) 463, 1246
Psp6I CCWGG 2 cut(s) 1197, 1251
PspEI GGTNACC 1 cut(s) 780
PspGI CCWGG 2 cut(s) 1197, 1251
PspN4I GGNNCC 4 cut(s) 9, 124, 399, 1248
PspPI GGNCC 3 cut(s) 273, 463, 1246
PspPPI RGGWCCY 2 cut(s) 463, 1246
PspXI VCTCGAGB 1 cut(s) 28
PsrI GAACNNNNNNTAC 2 cut(s) 1215, 1247
PsuI RGATCY 2 cut(s) 7, 397
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 5 cut(s) 25, 68, 335, 840, 1292
RsaNI GTAC 5 cut(s) 24, 67, 334, 839, 1291
RseI CAYNNNNRTG 1 cut(s) 550
SaqAI TTAA 6 cut(s) 533, 762, 1002, 1076, 1110, 1179
SatI GCNGC 3 cut(s) 171, 987, 990
Sau3AI GATC 6 cut(s) 7, 35, 105, 397, 910, 970
Sau96I GGNCC 3 cut(s) 273, 463, 1246
SchI GAGTC 3 cut(s) 488, 933, 1198
ScrFI CCNGG 3 cut(s) 594, 1199, 1253
SfaNI GCATC 2 cut(s) 157, 649
Sfr274I CTCGAG 1 cut(s) 28
SinI GGWCC 3 cut(s) 273, 463, 1246
SlaI CTCGAG 1 cut(s) 28
SmiI ATTTAAAT 1 cut(s) 1111
SmiMI CAYNNNNRTG 1 cut(s) 550
SmlI CTYRAG 3 cut(s) 28, 824, 1075
SmoI CTYRAG 3 cut(s) 28, 824, 1075
SphI GCATGC 1 cut(s) 1151
SsiI CCGC 6 cut(s) 33, 74, 327, 395, 718, 936
SspI AATATT 2 cut(s) 1107, 1115
SspMI CTAG 2 cut(s) 585, 723
StyD4I CCNGG 3 cut(s) 592, 1197, 1251
StyI CCWWGG 2 cut(s) 252, 545
SwaI ATTTAAAT 1 cut(s) 1111
TaaI ACNGT 5 cut(s) 66, 780, 884, 902, 1213
TaiI ACGT 2 cut(s) 72, 1224
TaqI TCGA 2 cut(s) 29, 515
TaqII GACCGA 1 cut(s) 705
TatI WGTACW 2 cut(s) 333, 838
TfiI GAWTC 1 cut(s) 752
Tru1I TTAA 6 cut(s) 533, 762, 1002, 1076, 1110, 1179
Tru9I TTAA 6 cut(s) 533, 762, 1002, 1076, 1110, 1179
TscAI CASTG 4 cut(s) 18, 489, 887, 1222
TseFI GTSAC 3 cut(s) 484, 1217, 1255
TseI GCWGC 3 cut(s) 170, 986, 989
Tsp45I GTSAC 3 cut(s) 484, 1217, 1255
TspDTI ATGAA 5 cut(s) 153, 296, 681, 1188, 1332
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 4 cut(s) 18, 489, 887, 1222
Van91I CCANNNNNTGG 1 cut(s) 258
Vha464I CTTAAG 1 cut(s) 1075
VpaK11BI GGWCC 3 cut(s) 273, 463, 1246
XapI RAATTY 4 cut(s) 82, 140, 1026, 1175
XceI RCATGY 2 cut(s) 340, 1151
XcmI CCANNNNNNNNNTGG 1 cut(s) 1279
XhoI CTCGAG 1 cut(s) 28
XspI CTAG 2 cut(s) 585, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.