RLG00000013988

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002805
Physical Location & Seq
Forward (+)
45205847 .. 45214881
9035 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013988

Sequence Viewer

Length: 1383 bp
ATGGAGCGGCACTCCAAGAATGAAGCGCTGGCAGAGTACATGTTGAGTAAGTGGCAGGAAATGGCAGACAAGAAGCCCAAAGGAATCTCGGAAAACATTGAAAAGACTCTTACTAAGGCGTACTCCAATGTCTGCAACTCTAAGAACCCTGTTAAAACCCTCAAAGACTTTTCCCAAATCAAGTGTGTTATAAGCCTGTTCTTTTTAATGCCAGAGAAGGGAAAGGGAATACCAGTACACCTGGGAAATTCTACAAGGGAATGGTATAGCGGTTGGTCCTGCATGAAGACATTGACAACCAAGGGACTGGTTGTGAAATCAAGTTGCCCAGCAAAGTACATGCTTACTCAAGAAGGTAAGGAGGCTGCTCGTGAATGTCTCATGAGGTCTGGTTTGGTGGATCCTGTAGAAATAATTTCTAATGCCAACGAGTTGTCTATCGGGGATGTGGACATTTCACATCAGGAACTTTCTAGTCCTGACTTGGCCACAGATATGACATTGTTCTCCTGTAATTCAAGCAGGAATAAAAACTCAATTGATATTCCACAGGAATATCTTGACAAGTTTACATGCATGGGGTATTCTGAAGAGCAGATGAACCGAGCTATCAGTGAATCTCAAAGCATAAGGGGAATTCTTCGGGCAACACCATCCACTCATACTCTTGTGAATGGTGATAGAGATTGTGTAGAGAGTGAGAGTAGACTGCCCAACACTTCTAGGGATGGAGGTCCTACGCCAAGAATTAATTCAGACAAGGTTGCATCAGAGTCGTTTACGATGGGAGCTCCTTCATCTCCTAGTTGTTCTGCTCCAAAGTCTAGTTTGGCTGGTGTTGAAGCAAGTTCGAAAGCTCTGTCAGTACCACCTTTAAGCTTTGGGGAGAAATTTGAAGATGCATACAAACTAGTCTTTGTATTGGATGATCGAGAACAATTGGCAGCTCAGATATCATGGTCTAGGAGCATTATCGAGAGTGTTATTTCTGGATGCAAAATCACAGTAGAGGTTAGGCGGTTACCAGTTGGAGACGGAATTTGGATAGCTCGGCACAAAAATCTGAAGAGTGAATATGTACTTGATTTTATTGTTGAGAGAAAGAATGTTGATGATTTGCGCTGTTCAATCAGAGACAACCGTTACAGGGATAAAAAATTAAGACTTTTGCGGTGTGGACTTAAGAAGCTGATATATCTCGTGGAAGGTGATCCAAATTCTAGTGAAGCAGTAGAAAGCATCAAAACAGCTTGCTTTACAACCGAACTTTTGGAGGGCTTTGATGTTCAGAGAACCGGAAACCTGACTGAAACCTTGAGGAACTCATGCAGGTTTAGGGACATGGAAGTCATCCCGTATTTGACAGTCAGGTCCCCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

461

Amino Acids

51.41

Weight (kDa)

8.1

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERCC4 PF02732 329 - 440 7.6e-22 ERCC4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 191
AasI GACNNNNNNGTC 2 cut(s) 1346, 1369
Acc36I ACCTGC 1 cut(s) 1320
AccBSI CCGCTC 1 cut(s) 7
AccI GTMKAC 1 cut(s) 706
AciI CCGC 4 cut(s) 7, 270, 1018, 1171
AclWI GGATC 3 cut(s) 395, 408, 1205
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 5 cut(s) 247, 636, 890, 1038, 1216
AcuI CTGAAG 2 cut(s) 609, 1085
AfaI GTAC 6 cut(s) 38, 122, 237, 338, 867, 1080
AfeI AGCGCT 1 cut(s) 27
AfiI CCNNNNNNNGG 2 cut(s) 218, 1147
AflII CTTAAG 1 cut(s) 1181
AflIII ACRYGT 1 cut(s) 39
AgsI TTSAA 5 cut(s) 101, 519, 842, 896, 1128
AhlI ACTAGT 1 cut(s) 910
AjnI CCWGG 1 cut(s) 240
AjuI GAANNNNNNNTTGG 2 cut(s) 736, 768
AluBI AGCT 8 cut(s) 608, 791, 857, 879, 947, 1049, 1189, 1250
AluI AGCT 8 cut(s) 608, 791, 857, 879, 947, 1049, 1189, 1250
Alw21I GWGCWC 1 cut(s) 793
Alw26I GTCTC 3 cut(s) 383, 1026, 1128
AlwI GGATC 3 cut(s) 395, 408, 1205
Aor51HI AGCGCT 1 cut(s) 27
AoxI GGCC 1 cut(s) 486
ApeKI GCWGC 2 cut(s) 365, 944
ApoI RAATTY 5 cut(s) 247, 636, 890, 1038, 1216
ArsI GACNNNNNNTTYG 2 cut(s) 845, 877
AseI ATTAAT 1 cut(s) 750
Asp700I GAANNNNTTC 1 cut(s) 751
AspLEI GCGC 2 cut(s) 28, 1122
AspS9I GGNCC 3 cut(s) 276, 734, 1371
AsuHPI GGTGA 2 cut(s) 689, 1220
AsuII TTCGAA 1 cut(s) 851
AvaII GGWCC 3 cut(s) 276, 734, 1371
BalI TGGCCA 1 cut(s) 488
BamHI GGATCC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 793
BauI CACGAG 2 cut(s) 369, 1199
BbsI GAAGAC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 793
BbvI GCAGC 2 cut(s) 352, 956
BccI CCATC 3 cut(s) 661, 722, 778
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciT130I CCWGG 1 cut(s) 242
BcoDI GTCTC 3 cut(s) 383, 1026, 1128
BcuI ACTAGT 1 cut(s) 910
BfaI CTAG 7 cut(s) 474, 723, 804, 825, 911, 963, 1221
BfmI CTRYAG 1 cut(s) 405
BfoI RGCGCY 1 cut(s) 29
BfrI CTTAAG 1 cut(s) 1181
BfuAI ACCTGC 1 cut(s) 1320
BisI GCNGC 3 cut(s) 8, 366, 945
BlsI GCNGC 3 cut(s) 9, 367, 946
Bme1390I CCNGG 1 cut(s) 242
Bme18I GGWCC 3 cut(s) 276, 734, 1371
BmgT120I GGNCC 3 cut(s) 276, 734, 1371
BmiI GGNNCC 2 cut(s) 402, 1373
BmrFI CCNGG 1 cut(s) 242
BmsI GCATC 4 cut(s) 776, 889, 983, 1248
BpiI GAAGAC 1 cut(s) 293
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu14I TTCGAA 1 cut(s) 851
BpuEI CTTGAG 2 cut(s) 333, 1336
BsaJI CCNNGG 2 cut(s) 241, 300
BsaWI WCCGGW 1 cut(s) 1295
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 1147
Bse1I ACTGG 3 cut(s) 233, 312, 1025
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 2 cut(s) 241, 300
BseGI GGATG 6 cut(s) 451, 653, 733, 931, 998, 1350
BseLI CCNNNNNNNGG 2 cut(s) 218, 1147
BseMII CTCAG 1 cut(s) 962
BseNI ACTGG 3 cut(s) 233, 312, 1025
BseXI GCAGC 2 cut(s) 352, 956
BseYI CCCAGC 1 cut(s) 328
BshFI GGCC 1 cut(s) 488
BsiHKAI GWGCWC 1 cut(s) 793
BsiSI CCGG 1 cut(s) 1296
BslFI GGGAC 3 cut(s) 318, 1352, 1357
BslI CCNNNNNNNGG 2 cut(s) 218, 1147
BsmAI GTCTC 3 cut(s) 383, 1026, 1128
BsmBI CGTCTC 1 cut(s) 1026
BsmFI GGGAC 3 cut(s) 318, 1352, 1357
BsnI GGCC 1 cut(s) 488
Bsp119I TTCGAA 1 cut(s) 851
Bsp1286I GDGCHC 1 cut(s) 793
Bsp143I GATC 3 cut(s) 400, 928, 1210
BspACI CCGC 4 cut(s) 7, 270, 1018, 1171
BspANI GGCC 1 cut(s) 488
BspCNI CTCAG 1 cut(s) 961
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 2 cut(s) 402, 1373
BspMI ACCTGC 1 cut(s) 1320
BspPI GGATC 3 cut(s) 395, 408, 1205
BspQI GCTCTTC 1 cut(s) 585
BspT104I TTCGAA 1 cut(s) 851
BspTI CTTAAG 1 cut(s) 1181
BsrBI CCGCTC 1 cut(s) 7
BsrI ACTGG 3 cut(s) 233, 312, 1025
BssECI CCNNGG 2 cut(s) 241, 300
BssMI GATC 3 cut(s) 400, 928, 1210
BssSI CACGAG 2 cut(s) 369, 1199
BssT1I CCWWGG 1 cut(s) 300
Bst2BI CACGAG 2 cut(s) 369, 1199
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 3 cut(s) 1006, 1142, 1366
Bst6I CTCTTC 2 cut(s) 585, 1061
BstAFI CTTAAG 1 cut(s) 1181
BstBI TTCGAA 1 cut(s) 851
BstC8I GCNNGC 2 cut(s) 30, 1252
BstDEI CTNAG 3 cut(s) 114, 141, 948
BstEII GGTNACC 1 cut(s) 1020
BstF5I GGATG 6 cut(s) 451, 653, 733, 931, 998, 1350
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 2 cut(s) 28, 1122
BstKTI GATC 3 cut(s) 403, 931, 1213
BstMAI GTCTC 3 cut(s) 383, 1026, 1128
BstMBI GATC 3 cut(s) 400, 928, 1210
BstNI CCWGG 1 cut(s) 242
BstNSI RCATGY 3 cut(s) 43, 343, 576
BstPI GGTNACC 1 cut(s) 1020
BstSCI CCNGG 1 cut(s) 240
BstSFI CTRYAG 1 cut(s) 405
BstV1I GCAGC 2 cut(s) 352, 956
BstV2I GAAGAC 1 cut(s) 293
BstX2I RGATCY 1 cut(s) 400
BstXI CCANNNNNNTGG 1 cut(s) 307
BstYI RGATCY 1 cut(s) 400
BsuRI GGCC 1 cut(s) 488
BtsCI GGATG 6 cut(s) 451, 653, 733, 931, 998, 1350
BtsIMutI CAGTG 1 cut(s) 619
BveI ACCTGC 1 cut(s) 1320
Cac8I GCNNGC 2 cut(s) 30, 1252
CciI TCATGA 1 cut(s) 381
CfoI GCGC 2 cut(s) 28, 1122
Cfr13I GGNCC 3 cut(s) 276, 734, 1371
Csp6I GTAC 6 cut(s) 37, 121, 236, 337, 866, 1079
CviAII CATG 9 cut(s) 40, 283, 340, 382, 573, 577, 957, 1326, 1342
CviQI GTAC 6 cut(s) 37, 121, 236, 337, 866, 1079
DdeI CTNAG 3 cut(s) 114, 141, 948
DpnI GATC 3 cut(s) 402, 930, 1212
DpnII GATC 3 cut(s) 400, 928, 1210
DrdI GACNNNNNNGTC 2 cut(s) 1346, 1369
DseDI GACNNNNNNGTC 2 cut(s) 1346, 1369
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 2 cut(s) 585, 1061
EarI CTCTTC 2 cut(s) 585, 1061
Ecl136II GAGCTC 1 cut(s) 791
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 793
Eco32I GATATC 1 cut(s) 954
Eco47I GGWCC 3 cut(s) 276, 734, 1371
Eco47III AGCGCT 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 791
Eco57I CTGAAG 2 cut(s) 609, 1085
Eco91I GGTNACC 1 cut(s) 1020
EcoICRI GAGCTC 1 cut(s) 791
EcoO109I RGGNCCY 2 cut(s) 734, 1371
EcoO65I GGTNACC 1 cut(s) 1020
EcoRI GAATTC 1 cut(s) 636
EcoRII CCWGG 1 cut(s) 240
EcoRV GATATC 1 cut(s) 954
EcoT14I CCWWGG 1 cut(s) 300
EcoT22I ATGCAT 2 cut(s) 578, 904
EcoT38I GRGCYC 1 cut(s) 793
ErhI CCWWGG 1 cut(s) 300
Esp3I CGTCTC 1 cut(s) 1026
FaeI CATG 9 cut(s) 43, 286, 343, 385, 576, 580, 960, 1329, 1345
FaqI GGGAC 3 cut(s) 318, 1352, 1357
FatI CATG 9 cut(s) 39, 282, 339, 381, 572, 576, 956, 1325, 1341
FblI GTMKAC 1 cut(s) 706
Fnu4HI GCNGC 3 cut(s) 8, 366, 945
FokI GGATG 6 cut(s) 458, 640, 740, 938, 1005, 1337
FriOI GRGCYC 1 cut(s) 793
Fsp4HI GCNGC 3 cut(s) 8, 366, 945
FspBI CTAG 7 cut(s) 474, 723, 804, 825, 911, 963, 1221
GlaI GCGC 2 cut(s) 27, 1121
GluI GCNGC 3 cut(s) 8, 366, 945
GsaI CCCAGC 1 cut(s) 332
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 1 cut(s) 488
HapII CCGG 1 cut(s) 1296
HhaI GCGC 2 cut(s) 28, 1122
Hin1II CATG 9 cut(s) 43, 286, 343, 385, 576, 580, 960, 1329, 1345
Hin6I GCGC 2 cut(s) 26, 1120
HinP1I GCGC 2 cut(s) 26, 1120
HindIII AAGCTT 1 cut(s) 877
HinfI GANTC 4 cut(s) 84, 106, 617, 773
HpaII CCGG 1 cut(s) 1296
HphI GGTGA 2 cut(s) 689, 1220
Hpy166II GTNNAC 6 cut(s) 238, 451, 570, 707, 780, 1178
Hpy188I TCNGA 8 cut(s) 91, 589, 757, 772, 951, 1065, 1133, 1290
Hpy188III TCNNGA 9 cut(s) 350, 371, 382, 464, 479, 560, 932, 976, 990
Hpy8I GTNNAC 6 cut(s) 238, 451, 570, 707, 780, 1178
HpyAV CCTTC 4 cut(s) 211, 347, 804, 1199
HpyCH4III ACNGT 3 cut(s) 1006, 1142, 1366
HpyCH4V TGCA 7 cut(s) 135, 282, 576, 767, 902, 996, 1329
HpyF3I CTNAG 3 cut(s) 114, 141, 948
Hsp92II CATG 9 cut(s) 43, 286, 343, 385, 576, 580, 960, 1329, 1345
HspAI GCGC 2 cut(s) 26, 1120
Kzo9I GATC 3 cut(s) 400, 928, 1210
LguI GCTCTTC 1 cut(s) 585
LmnI GCTCC 5 cut(s) 4, 788, 796, 820, 966
Lsp1109I GCAGC 2 cut(s) 352, 956
LweI GCATC 4 cut(s) 776, 889, 983, 1248
MaeI CTAG 7 cut(s) 474, 723, 804, 825, 911, 963, 1221
MaeIII GTNAC 2 cut(s) 1020, 1142
MalI GATC 3 cut(s) 402, 930, 1212
MbiI CCGCTC 1 cut(s) 7
MboI GATC 3 cut(s) 400, 928, 1210
MboII GAAGA 5 cut(s) 298, 602, 632, 908, 1078
MfeI CAATTG 2 cut(s) 537, 938
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 1 cut(s) 793
MlsI TGGCCA 1 cut(s) 488
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 2 cut(s) 100, 782
MmeI TCCRAC 1 cut(s) 1009
MnlI CCTC 7 cut(s) 170, 355, 378, 725, 1003, 1267, 1311
Mox20I TGGCCA 1 cut(s) 488
Mph1103I ATGCAT 2 cut(s) 578, 904
MroXI GAANNNNTTC 1 cut(s) 751
MscI TGGCCA 1 cut(s) 488
MseI TTAA 6 cut(s) 153, 206, 750, 875, 1160, 1182
MslI CAYNNNNRTG 1 cut(s) 494
Msp20I TGGCCA 1 cut(s) 488
MspCI CTTAAG 1 cut(s) 1181
MspI CCGG 1 cut(s) 1296
MspR9I CCNGG 1 cut(s) 242
MunI CAATTG 2 cut(s) 537, 938
MvaI CCWGG 1 cut(s) 242
NdeII GATC 3 cut(s) 400, 928, 1210
NlaIII CATG 9 cut(s) 43, 286, 343, 385, 576, 580, 960, 1329, 1345
NlaIV GGNNCC 2 cut(s) 402, 1373
NmeAIII GCCGAG 1 cut(s) 1030
NsiI ATGCAT 2 cut(s) 578, 904
NspI RCATGY 3 cut(s) 43, 343, 576
NspV TTCGAA 1 cut(s) 851
PagI TCATGA 1 cut(s) 381
PciI ACATGT 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 585
PdmI GAANNNNTTC 1 cut(s) 751
PfeI GAWTC 2 cut(s) 84, 617
PkrI GCNGC 3 cut(s) 9, 367, 946
PleI GAGTC 2 cut(s) 100, 781
PpsI GAGTC 2 cut(s) 100, 781
PpuMI RGGWCCY 2 cut(s) 734, 1371
PscI ACATGT 1 cut(s) 39
PshBI ATTAAT 1 cut(s) 750
PsiI TTATAA 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 793
Psp5II RGGWCCY 2 cut(s) 734, 1371
Psp6I CCWGG 1 cut(s) 240
PspEI GGTNACC 1 cut(s) 1020
PspFI CCCAGC 1 cut(s) 328
PspGI CCWGG 1 cut(s) 240
PspN4I GGNNCC 2 cut(s) 402, 1373
PspPI GGNCC 3 cut(s) 276, 734, 1371
PspPPI RGGWCCY 2 cut(s) 734, 1371
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 6 cut(s) 38, 122, 237, 338, 867, 1080
RsaNI GTAC 6 cut(s) 37, 121, 236, 337, 866, 1079
RseI CAYNNNNRTG 1 cut(s) 494
SacI GAGCTC 1 cut(s) 793
SapI GCTCTTC 1 cut(s) 585
SaqAI TTAA 6 cut(s) 153, 206, 750, 875, 1160, 1182
SatI GCNGC 3 cut(s) 8, 366, 945
Sau3AI GATC 3 cut(s) 400, 928, 1210
Sau96I GGNCC 3 cut(s) 276, 734, 1371
SchI GAGTC 2 cut(s) 100, 782
ScrFI CCNGG 1 cut(s) 242
SduI GDGCHC 1 cut(s) 793
SfaNI GCATC 4 cut(s) 776, 889, 983, 1248
SfcI CTRYAG 1 cut(s) 405
SfuI TTCGAA 1 cut(s) 851
SinI GGWCC 3 cut(s) 276, 734, 1371
SmiMI CAYNNNNRTG 1 cut(s) 494
SmlI CTYRAG 3 cut(s) 348, 1181, 1315
SmoI CTYRAG 3 cut(s) 348, 1181, 1315
SpeI ACTAGT 1 cut(s) 910
SsiI CCGC 4 cut(s) 7, 270, 1018, 1171
SspMI CTAG 7 cut(s) 474, 723, 804, 825, 911, 963, 1221
SstI GAGCTC 1 cut(s) 793
StyD4I CCNGG 1 cut(s) 240
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 3 cut(s) 1006, 1142, 1366
TaqI TCGA 3 cut(s) 851, 931, 975
TatI WGTACW 4 cut(s) 36, 235, 336, 1078
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 2 cut(s) 84, 617
Tru1I TTAA 6 cut(s) 153, 206, 750, 875, 1160, 1182
Tru9I TTAA 6 cut(s) 153, 206, 750, 875, 1160, 1182
TscAI CASTG 1 cut(s) 619
TseI GCWGC 2 cut(s) 365, 944
TspDTI ATGAA 4 cut(s) 36, 299, 614, 786
TspGWI ACGGA 1 cut(s) 1050
TspRI CASTG 1 cut(s) 619
Vha464I CTTAAG 1 cut(s) 1181
VpaK11BI GGWCC 3 cut(s) 276, 734, 1371
VspI ATTAAT 1 cut(s) 750
XapI RAATTY 5 cut(s) 247, 636, 890, 1038, 1216
XceI RCATGY 3 cut(s) 43, 343, 576
XmiI GTMKAC 1 cut(s) 706
XmnI GAANNNNTTC 1 cut(s) 751
XspI CTAG 7 cut(s) 474, 723, 804, 825, 911, 963, 1221
Zsp2I ATGCAT 2 cut(s) 578, 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.