RchiOBHm_Chr3g0488831

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002805
Physical Location & Seq
Forward (+)
36538384 .. 36546666
8283 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45314

Sequence Viewer

Length: 1434 bp
ATGGATTTCCTCTTTTTTTTCGTAGCCAAGAGAACCAAATCAACCAAACGGTACGTTCCGCAGAAGAATTCCGTGGCATATGCTTTGTTGATCACGCTTTACAGGTTAAATGCTTTCATCAGGGGAACCGAAAATGGAGATGAATTTATGCGTAAACAGGAGCTTATTGATGCAGCTGAAGCAAGTGGACTTTCTCGTGTGCCGATTATGTATGGGAAGGGAAAGCCTACAACTCTCGGCAATTCTGCCAAGGAATGGTATAGTGGTTGGTCCTGCATGAAGACATTGATAACCAAAGGACTGGTTCTGAAATCAAGTTGCCCCGCAAAGTACATGCTTACTGAAGAAGGTCAGGAAGTTGCCCGTGAATGTCTCATGAGGTCTGGTTTGGCGGATCCTGTGGAAAAAGTTTCTAATGCCAAAGGGTTGTCTGTCGTGGATGTGAATGTATCAGAACAGGACCTTTCTCATCCTGAGTCAGTGACAGATATGACATTGTTGTCCAGTAATTCGAGTAGTGAGGAGAACTTAATTGATATGCCATGGGAATGTCTTGACAAGAGTTGTCCCATGCCAAAGTCTAGTTCTCCGGGTGTCAAAGCAAGTTCAAAAGCTTTATCCATTCCACCTCTAAGCTTTGGGGAGAGATTTGAAGATGCTTATGAAGTAGTCTTTCTATTAGATGACCGAGAGCAATTGGCCACTCAGATATTGCGGTCTAGGAGCATTATTGAGAGTGTTATTTCTGAATCCAAAATTAAAGTAGAGGTCAGACGGTTACCAGTTGGAGATGGGATTTGGATAGCTCGCCATAATTATCTTGAGACTGAATATGTACTTGATTTTATTGTTGAGAGGAAGAATGTTGATGATTTACACTGTTCAATAAGAGACAACCGTTACAGGGATCAAAAATTGAGACTTTTGAGGTGCGGACTCAAAAAGCTGATATATCTTGTGGAAGGTGATCCCAATTCTTGTGCAGCAGCAGAAAGCATTAAAACAGCCTGCTTCACAACTGAAATTTTGGAGGGTTTTGATGTTCAGAGAACTGAAAACTTGACTGAAACCTTAAGGAAATATGGTTATCTTACTGAAGCAATATTTAAATATTACAAAGAACTTCCTGAGGAACAGCGTAAGCATGCTGGAGTTTGTCTTACATTTGATGAATTTAACAAAAGGAGTCAAGACCTGGATAAAATGACAGTCAGTGACGTATTTTCTGTTCAGCTCATGCAGGTCCCCCAGGTGACTGAGGAAGTTGCCATTGCTGTTCTGGATTTGTACCCAACGCCTTTTTCTCTTGCCCATGAATACTCTCTACTTGAGGGTGATGTCCGCACACAGGAGGAGATGCTTAGAACACAGAGTAACAATGTTGTAAACGCTGCTGCCAGTACGAATATTTTTCGCTTTGTATGGAGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

477

Amino Acids

54.05

Weight (kDa)

5.42

Isoelectric Point (pI)

51.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_MUS81 PF21136 20 - 122 5.8e-13 Crossover junction endonuclease MUS81-like, winged helix domain
ERCC4 PF02732 228 - 358 3e-24 ERCC4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 499
Acc36I ACCTGC 1 cut(s) 1231
AccB7I CCANNNNNTGG 1 cut(s) 255
AciI CCGC 6 cut(s) 59, 324, 392, 715, 933, 1342
AclWI GGATC 4 cut(s) 389, 402, 915, 962
AcoI YGGCCR 1 cut(s) 699
AcsI RAATTY 4 cut(s) 67, 143, 1023, 1172
AcuI CTGAAG 3 cut(s) 198, 363, 1116
AfaI GTAC 5 cut(s) 53, 332, 837, 1289, 1402
AfiI CCNNNNNNNGG 3 cut(s) 255, 904, 1348
AflII CTTAAG 1 cut(s) 1072
AgsI TTSAA 3 cut(s) 609, 653, 885
AjnI CCWGG 2 cut(s) 1194, 1248
AjuI GAANNNNNNNTTGG 4 cut(s) 568, 600, 1285, 1317
AluBI AGCT 7 cut(s) 163, 176, 614, 636, 806, 946, 1234
AluI AGCT 7 cut(s) 163, 176, 614, 636, 806, 946, 1234
Alw26I GTCTC 4 cut(s) 377, 818, 885, 913
AlwI GGATC 4 cut(s) 389, 402, 915, 962
AoxI GGCC 1 cut(s) 699
ApeKI GCWGC 5 cut(s) 173, 983, 986, 1391, 1394
ApoI RAATTY 4 cut(s) 67, 143, 1023, 1172
AspS9I GGNCC 3 cut(s) 270, 460, 1243
AsuC2I CCSGG 1 cut(s) 591
AsuHPI GGTGA 3 cut(s) 977, 1264, 1346
AvaII GGWCC 3 cut(s) 270, 460, 1243
AxyI CCTNAGG 1 cut(s) 1128
BalI TGGCCA 1 cut(s) 701
BamHI GGATCC 1 cut(s) 394
BauI CACGAG 1 cut(s) 195
BbsI GAAGAC 1 cut(s) 287
BbvI GCAGC 5 cut(s) 185, 995, 998, 1378, 1381
BccI CCATC 1 cut(s) 785
BciT130I CCWGG 2 cut(s) 1196, 1250
BclI TGATCA 1 cut(s) 90
BcnI CCSGG 1 cut(s) 591
BcoDI GTCTC 4 cut(s) 377, 818, 885, 913
BfaI CTAG 2 cut(s) 582, 720
BfrI CTTAAG 1 cut(s) 1072
BfuAI ACCTGC 1 cut(s) 1231
BisI GCNGC 5 cut(s) 174, 984, 987, 1392, 1395
BlsI GCNGC 5 cut(s) 175, 985, 988, 1393, 1396
Bme1390I CCNGG 3 cut(s) 591, 1196, 1250
Bme18I GGWCC 3 cut(s) 270, 460, 1243
BmgT120I GGNCC 3 cut(s) 270, 460, 1243
BmiI GGNNCC 3 cut(s) 127, 396, 1245
BmrFI CCNGG 3 cut(s) 591, 1196, 1250
BmsI GCATC 3 cut(s) 160, 646, 1347
BpiI GAAGAC 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 1170
BpuEI CTTGAG 2 cut(s) 842, 1349
BpuMI CCSGG 1 cut(s) 591
BsaJI CCNNGG 4 cut(s) 72, 249, 542, 1248
Bsc4I CCNNNNNNNGG 3 cut(s) 255, 904, 1348
Bse1I ACTGG 4 cut(s) 306, 504, 782, 1398
Bse21I CCTNAGG 1 cut(s) 1128
Bse3DI GCAATG 1 cut(s) 1269
BseBI CCWGG 2 cut(s) 1196, 1250
BseDI CCNNGG 4 cut(s) 72, 249, 542, 1248
BseGI GGATG 2 cut(s) 445, 469
BseLI CCNNNNNNNGG 3 cut(s) 255, 904, 1348
BseMI GCAATG 1 cut(s) 1269
BseMII CTCAG 4 cut(s) 465, 719, 1119, 1248
BseNI ACTGG 4 cut(s) 306, 504, 782, 1398
BseRI GAGGAG 2 cut(s) 536, 1367
BseXI GCAGC 5 cut(s) 185, 995, 998, 1378, 1381
BsgI GTGCAG 1 cut(s) 1002
BshFI GGCC 1 cut(s) 701
BsiSI CCGG 1 cut(s) 590
BslFI GGGAC 2 cut(s) 552, 1229
BslI CCNNNNNNNGG 3 cut(s) 255, 904, 1348
BsmAI GTCTC 4 cut(s) 377, 818, 885, 913
BsmFI GGGAC 2 cut(s) 552, 1229
BsnI GGCC 1 cut(s) 701
Bsp143I GATC 4 cut(s) 90, 394, 907, 967
Bsp19I CCATGG 1 cut(s) 542
BspACI CCGC 6 cut(s) 59, 324, 392, 715, 933, 1342
BspANI GGCC 1 cut(s) 701
BspCNI CTCAG 4 cut(s) 466, 718, 1120, 1249
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 3 cut(s) 127, 396, 1245
BspMI ACCTGC 1 cut(s) 1231
BspPI GGATC 4 cut(s) 389, 402, 915, 962
BspTI CTTAAG 1 cut(s) 1072
BsrDI GCAATG 1 cut(s) 1269
BsrI ACTGG 4 cut(s) 306, 504, 782, 1398
BssECI CCNNGG 4 cut(s) 72, 249, 542, 1248
BssMI GATC 4 cut(s) 90, 394, 907, 967
BssSI CACGAG 1 cut(s) 195
BssT1I CCWWGG 2 cut(s) 249, 542
Bst2BI CACGAG 1 cut(s) 195
Bst2UI CCWGG 2 cut(s) 1196, 1250
Bst4CI ACNGT 5 cut(s) 51, 777, 881, 899, 1210
BstAFI CTTAAG 1 cut(s) 1072
BstC8I GCNNGC 3 cut(s) 808, 1009, 1146
BstDEI CTNAG 6 cut(s) 474, 632, 705, 1128, 1257, 1361
BstDSI CCRYGG 2 cut(s) 72, 542
BstEII GGTNACC 1 cut(s) 777
BstF5I GGATG 2 cut(s) 445, 469
BstKTI GATC 4 cut(s) 93, 397, 910, 970
BstMAI GTCTC 4 cut(s) 377, 818, 885, 913
BstMBI GATC 4 cut(s) 90, 394, 907, 967
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 2 cut(s) 1196, 1250
BstNSI RCATGY 2 cut(s) 337, 1148
BstPI GGTNACC 1 cut(s) 777
BstSCI CCNGG 3 cut(s) 589, 1194, 1248
BstV1I GCAGC 5 cut(s) 185, 995, 998, 1378, 1381
BstV2I GAAGAC 1 cut(s) 287
BstX2I RGATCY 1 cut(s) 394
BstXI CCANNNNNNTGG 1 cut(s) 301
BstYI RGATCY 1 cut(s) 394
Bsu36I CCTNAGG 1 cut(s) 1128
BsuRI GGCC 1 cut(s) 701
BtgI CCRYGG 2 cut(s) 72, 542
BtsCI GGATG 2 cut(s) 445, 469
BtsIMutI CAGTG 3 cut(s) 486, 877, 1219
BveI ACCTGC 1 cut(s) 1231
Cac8I GCNNGC 3 cut(s) 808, 1009, 1146
CciI TCATGA 1 cut(s) 375
Cfr13I GGNCC 3 cut(s) 270, 460, 1243
Csp6I GTAC 5 cut(s) 52, 331, 836, 1288, 1401
CviAII CATG 8 cut(s) 277, 334, 376, 543, 571, 1145, 1237, 1313
CviQI GTAC 5 cut(s) 52, 331, 836, 1288, 1401
DdeI CTNAG 6 cut(s) 474, 632, 705, 1128, 1257, 1361
DpnI GATC 4 cut(s) 92, 396, 909, 969
DpnII GATC 4 cut(s) 90, 394, 907, 967
DraI TTTAAA 1 cut(s) 1108
DrdI GACNNNNNNGTC 1 cut(s) 499
DseDI GACNNNNNNGTC 1 cut(s) 499
EaeI YGGCCR 1 cut(s) 699
EciI GGCGGA 1 cut(s) 407
Eco130I CCWWGG 2 cut(s) 249, 542
Eco47I GGWCC 3 cut(s) 270, 460, 1243
Eco57I CTGAAG 3 cut(s) 198, 363, 1116
Eco81I CCTNAGG 1 cut(s) 1128
Eco91I GGTNACC 1 cut(s) 777
EcoO109I RGGNCCY 2 cut(s) 460, 1243
EcoO65I GGTNACC 1 cut(s) 777
EcoRI GAATTC 1 cut(s) 67
EcoRII CCWGG 2 cut(s) 1194, 1248
EcoT14I CCWWGG 2 cut(s) 249, 542
ErhI CCWWGG 2 cut(s) 249, 542
FaeI CATG 8 cut(s) 280, 337, 379, 546, 574, 1148, 1240, 1316
FaqI GGGAC 2 cut(s) 552, 1229
FatI CATG 8 cut(s) 276, 333, 375, 542, 570, 1144, 1236, 1312
FauI CCCGC 1 cut(s) 331
FauNDI CATATG 1 cut(s) 79
FbaI TGATCA 1 cut(s) 90
Fnu4HI GCNGC 5 cut(s) 174, 984, 987, 1392, 1395
FokI GGATG 2 cut(s) 452, 456
Fsp4HI GCNGC 5 cut(s) 174, 984, 987, 1392, 1395
FspBI CTAG 2 cut(s) 582, 720
GluI GCNGC 5 cut(s) 174, 984, 987, 1392, 1395
GsuI CTGGAG 1 cut(s) 1170
HaeIII GGCC 1 cut(s) 701
HapII CCGG 1 cut(s) 590
Hin1II CATG 8 cut(s) 280, 337, 379, 546, 574, 1148, 1240, 1316
HindIII AAGCTT 2 cut(s) 612, 634
HinfI GANTC 4 cut(s) 476, 749, 936, 1186
HpaII CCGG 1 cut(s) 590
HphI GGTGA 3 cut(s) 977, 1264, 1346
Hpy166II GTNNAC 3 cut(s) 155, 188, 1387
Hpy188I TCNGA 6 cut(s) 309, 454, 708, 748, 773, 1047
Hpy188III TCNNGA 8 cut(s) 353, 376, 473, 554, 821, 1127, 1190, 1280
Hpy8I GTNNAC 3 cut(s) 155, 188, 1387
HpyAV CCTTC 3 cut(s) 211, 341, 956
HpyCH4III ACNGT 5 cut(s) 51, 777, 881, 899, 1210
HpyCH4IV ACGT 2 cut(s) 54, 1218
HpyCH4V TGCA 4 cut(s) 173, 276, 983, 1240
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 6 cut(s) 474, 632, 705, 1128, 1257, 1361
HpySE526I ACGT 2 cut(s) 54, 1218
Hsp92II CATG 8 cut(s) 280, 337, 379, 546, 574, 1148, 1240, 1316
Ksp22I TGATCA 1 cut(s) 90
Kzo9I GATC 4 cut(s) 90, 394, 907, 967
LmnI GCTCC 3 cut(s) 160, 723, 1425
Lsp1109I GCAGC 5 cut(s) 185, 995, 998, 1378, 1381
LweI GCATC 3 cut(s) 160, 646, 1347
MaeI CTAG 2 cut(s) 582, 720
MaeII ACGT 2 cut(s) 54, 1218
MaeIII GTNAC 6 cut(s) 481, 777, 899, 1214, 1252, 1373
MalI GATC 4 cut(s) 92, 396, 909, 969
MboI GATC 4 cut(s) 90, 394, 907, 967
MboII GAAGA 5 cut(s) 76, 292, 356, 665, 871
MfeI CAATTG 2 cut(s) 695, 1429
MflI RGATCY 1 cut(s) 394
MlsI TGGCCA 1 cut(s) 701
MluNI TGGCCA 1 cut(s) 701
MlyI GAGTC 3 cut(s) 485, 930, 1195
MmeI TCCRAC 1 cut(s) 766
Mox20I TGGCCA 1 cut(s) 701
MscI TGGCCA 1 cut(s) 701
MseI TTAA 7 cut(s) 107, 530, 759, 999, 1073, 1107, 1176
MslI CAYNNNNRTG 1 cut(s) 547
Msp20I TGGCCA 1 cut(s) 701
MspA1I CMGCKG 1 cut(s) 176
MspCI CTTAAG 1 cut(s) 1072
MspI CCGG 1 cut(s) 590
MspR9I CCNGG 3 cut(s) 591, 1196, 1250
MunI CAATTG 2 cut(s) 695, 1429
MvaI CCWGG 2 cut(s) 1196, 1250
MwoI GCNNNNNNNGC 1 cut(s) 179
NciI CCSGG 1 cut(s) 591
NcoI CCATGG 1 cut(s) 542
NdeI CATATG 1 cut(s) 79
NdeII GATC 4 cut(s) 90, 394, 907, 967
NlaIII CATG 8 cut(s) 280, 337, 379, 546, 574, 1148, 1240, 1316
NlaIV GGNNCC 3 cut(s) 127, 396, 1245
NmeAIII GCCGAG 1 cut(s) 216
NmuCI GTSAC 3 cut(s) 481, 1214, 1252
NspI RCATGY 2 cut(s) 337, 1148
PaeI GCATGC 1 cut(s) 1148
PagI TCATGA 1 cut(s) 375
PfeI GAWTC 1 cut(s) 749
PflMI CCANNNNNTGG 1 cut(s) 255
PkrI GCNGC 5 cut(s) 175, 985, 988, 1393, 1396
PleI GAGTC 3 cut(s) 484, 930, 1194
PpsI GAGTC 3 cut(s) 484, 930, 1194
PpuMI RGGWCCY 2 cut(s) 460, 1243
Psp5II RGGWCCY 2 cut(s) 460, 1243
Psp6I CCWGG 2 cut(s) 1194, 1248
PspEI GGTNACC 1 cut(s) 777
PspGI CCWGG 2 cut(s) 1194, 1248
PspN4I GGNNCC 3 cut(s) 127, 396, 1245
PspPI GGNCC 3 cut(s) 270, 460, 1243
PspPPI RGGWCCY 2 cut(s) 460, 1243
PsrI GAACNNNNNNTAC 2 cut(s) 1212, 1244
PsuI RGATCY 1 cut(s) 394
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 5 cut(s) 53, 332, 837, 1289, 1402
RsaNI GTAC 5 cut(s) 52, 331, 836, 1288, 1401
RseI CAYNNNNRTG 1 cut(s) 547
SaqAI TTAA 7 cut(s) 107, 530, 759, 999, 1073, 1107, 1176
SatI GCNGC 5 cut(s) 174, 984, 987, 1392, 1395
Sau3AI GATC 4 cut(s) 90, 394, 907, 967
Sau96I GGNCC 3 cut(s) 270, 460, 1243
SchI GAGTC 3 cut(s) 485, 930, 1195
ScrFI CCNGG 3 cut(s) 591, 1196, 1250
SfaNI GCATC 3 cut(s) 160, 646, 1347
SinI GGWCC 3 cut(s) 270, 460, 1243
SmiI ATTTAAAT 1 cut(s) 1108
SmiMI CAYNNNNRTG 1 cut(s) 547
SmlI CTYRAG 3 cut(s) 821, 1072, 1328
SmoI CTYRAG 3 cut(s) 821, 1072, 1328
SphI GCATGC 1 cut(s) 1148
SsiI CCGC 6 cut(s) 59, 324, 392, 715, 933, 1342
SspI AATATT 3 cut(s) 1104, 1112, 1408
SspMI CTAG 2 cut(s) 582, 720
StyD4I CCNGG 3 cut(s) 589, 1194, 1248
StyI CCWWGG 2 cut(s) 249, 542
SwaI ATTTAAAT 1 cut(s) 1108
TaaI ACNGT 5 cut(s) 51, 777, 881, 899, 1210
TaiI ACGT 2 cut(s) 57, 1221
TaqI TCGA 1 cut(s) 512
TaqII GACCGA 1 cut(s) 702
TatI WGTACW 2 cut(s) 330, 835
TfiI GAWTC 1 cut(s) 749
Tru1I TTAA 7 cut(s) 107, 530, 759, 999, 1073, 1107, 1176
Tru9I TTAA 7 cut(s) 107, 530, 759, 999, 1073, 1107, 1176
TscAI CASTG 3 cut(s) 486, 884, 1219
TseFI GTSAC 3 cut(s) 481, 1214, 1252
TseI GCWGC 5 cut(s) 173, 983, 986, 1391, 1394
Tsp45I GTSAC 3 cut(s) 481, 1214, 1252
TspDTI ATGAA 6 cut(s) 106, 156, 293, 678, 1185, 1329
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 3 cut(s) 486, 884, 1219
Van91I CCANNNNNTGG 1 cut(s) 255
Vha464I CTTAAG 1 cut(s) 1072
VpaK11BI GGWCC 3 cut(s) 270, 460, 1243
XapI RAATTY 4 cut(s) 67, 143, 1023, 1172
XceI RCATGY 2 cut(s) 337, 1148
XcmI CCANNNNNNNNNTGG 1 cut(s) 1276
XspI CTAG 2 cut(s) 582, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.