RchiOBHm_Chr1g0319541

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002805
Physical Location & Seq
Forward (+)
7195199 .. 7207467
12269 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54978

Sequence Viewer

Length: 2010 bp
ATGGAGCGGCACAAGCGGTTGGTGGTGTGCTCTGAGAATGAAGCGCTGGCAGAGTACATGTTGAGTAAGTGGCAGGAAATGGCAGACAAGAAGCCCAAAGGAATCTCTGAAAACATTGAAAAGACTCTTACCAAGGCATACTCCAATGTCTGCAACTCTAAGAATCCTGTTAAAACCCTCAAAGAGTTTTCCCAAATCAAGGGTGTTGGGAAGTGGATTTTGAAGCTTATGCAAGGGTACTTTGACACCGGTTCAGGCAGTTCTGAACCAGAAGATGTAACTAAAAAGGGTAAGAGAACCAAAGGAAGCAAACGCTATGTTCCACAAAAGAATTCTGTAGCATATGCGTTACTGATCACACTTTACAGGGGAACTGAAAGTGGGAATGAATTTATGCGTAAACAGGAGCTCATTGATGCAGCTGAAGCAAGTGGACTATCTCGTGTTCCGATTATGCCAGAGAAGGGAAAGGGAATACCGGTACACCTGGGAAATTCTACAAGGGAATGGTATAGTGGTTGGTCCTGCATGAAGACATTGATAACCAAGGGACTGGTTGTGAAATCAAGTTGCCCAGCAAAGTACATGCTTACTCAAGAAGGTAAGGAGGCTGCTCGTGAATGTCTCGTGAGGTCTGGTTTGGCGGATCCTGTAGAAATAATTTCTAATGCCAGGGAGTTGTCTATCGGGGATGTGGACATTTCACATCAGGAACTTTCTAGTCCTGACTCGGCCACAGATATGACATTGTTATCCTGTAATTCAAGTAGGAATAAAAACTCAATTGATATTCCACAGGAATATCTTGACAAGTTTACACGCATGGGGTATTCTGAGGAACAGATGAACCGAGCTATCAGTGAAGTTTTAGAGACTTCCCATAACAAGGAAATCTCTTCACTATGGCCATCAGTTCTTTGTCGCCTTCGAGAAGATGAGGTTTATGGTTTTCATATGAAGTCTCAAAGCATAAGGGGAAATCTTCGGGCAACACCATCCACTCATACTCTTGTGAATGGTGATAGAGATTGTGTTAGACTGCCCAACACTTCTAGGGATGGAGGTCCTACGCCAAGAATTAATTCTGACAAGGTTGCATCAAAGTTGTTTACGACGGGAGCTCCTTCATCTCCTAGTTGTCCTGTGCCAAAGTCCAGTTTGGCTGGTGTTGAAGCAAGTTCGAAAGCTCTGTCTGTGCCACCTTTAAGCTTTGGGGAGAAATTTGAAGATGCATATGAACTAGTCTTTGTATTGGATGATCGAGAACAATTGGCCGCTCAGATATCACGGTCTAGGAGCATTATCGAGAGTGTTATTTCTGGATGCAAAATCACAGTAGAGGTTAGGCGGTTACCAGTTGGAGACGGAATTTGGATAGCTCGGCACAAACATCTGAAGAGTGAATATGTACTTGATTTTATTGTTGAGAGGAAAAATGTTGATGATTTGCGCTGTTCAATCAGAGACAACCGTTACAGGGATCAAAAATTGAGACTTTTGCGGTGTGGACTTAAGAAGCTCATATATCTCGTGGAAGGTGATCCAAATTCTAGTGAAGCAGCAGAAAGCATCAAAACAGCTTGCTTTACAACCGAGCTTTTGGAGGGCTTTGATGTTCAGAGAACGGGAAACCTGACTGAAACCTTGAGGAAGTATGGTTATCTTACTGAAGCAATTTTTAAATATTACAAAGATCTCTCTGAGGAACAGCATAAGCAAGCTGGAGTTTGTCCTCCTTTTGATGAATTTAACAAAAGGTGCCAAGACCTGGACAAGATGACAGTCAGTGACGTATTTGCCATTCAGCTCATGCAGGTCCCCCAGGTGACTGAGGAAGTTGCCATAGCTGTTCTGGATTTGTACCCAACAGTTATTTCTCTTGCCCGTGAATACTCTCTACTTGAGGGCGATGTTGGCGCACAGGAAGAGATGCTTAGAACGCAGAGTAACAATGTTGTAAATGCTGCTGCTAGTAAGAATATATTCCACTTTGTTTGGGGCATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

669

Amino Acids

74.88

Weight (kDa)

7.47

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_MUS81 PF21136 108 - 205 7.2e-12 Crossover junction endonuclease MUS81-like, winged helix domain
ERCC4 PF02732 439 - 549 2.1e-22 ERCC4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1804
AccB1I GGYRCC 1 cut(s) 1758
AccB7I CCANNNNNTGG 1 cut(s) 1768
AccBSI CCGCTC 2 cut(s) 7, 1277
AciI CCGC 6 cut(s) 7, 16, 644, 1275, 1348, 1501
AclWI GGATC 4 cut(s) 641, 654, 1488, 1535
AcoI YGGCCR 3 cut(s) 732, 905, 1272
AcsI RAATTY 7 cut(s) 331, 389, 493, 1220, 1368, 1546, 1745
AcuI CTGAAG 3 cut(s) 444, 1415, 1689
AfaI GTAC 6 cut(s) 56, 239, 483, 584, 1410, 1862
AfeI AGCGCT 1 cut(s) 45
AfiI CCNNNNNNNGG 5 cut(s) 199, 464, 886, 1477, 1768
AflII CTTAAG 1 cut(s) 1511
AflIII ACRYGT 1 cut(s) 57
AgeI ACCGGT 2 cut(s) 248, 478
AgsI TTSAA 6 cut(s) 119, 223, 765, 1172, 1226, 1458
AhlI ACTAGT 1 cut(s) 1240
AjnI CCWGG 4 cut(s) 486, 671, 1767, 1821
AjuI GAANNNNNNNTTGG 4 cut(s) 1066, 1098, 1858, 1890
Alw21I GWGCWC 3 cut(s) 32, 411, 1123
Alw26I GTCTC 6 cut(s) 629, 866, 966, 1356, 1458, 1486
AlwI GGATC 4 cut(s) 641, 654, 1488, 1535
Aor51HI AGCGCT 1 cut(s) 45
AoxI GGCC 3 cut(s) 732, 905, 1272
ApeKI GCWGC 5 cut(s) 419, 611, 1559, 1964, 1967
ApoI RAATTY 7 cut(s) 331, 389, 493, 1220, 1368, 1546, 1745
ArsI GACNNNNNNTTYG 2 cut(s) 1175, 1207
AseI ATTAAT 1 cut(s) 1080
AsiGI ACCGGT 2 cut(s) 248, 478
Asp700I GAANNNNTTC 2 cut(s) 1081, 1982
AspLEI GCGC 3 cut(s) 46, 1452, 1919
AspS9I GGNCC 3 cut(s) 522, 1064, 1816
AsuHPI GGTGA 3 cut(s) 1031, 1550, 1837
AsuII TTCGAA 1 cut(s) 1181
AvaII GGWCC 3 cut(s) 522, 1064, 1816
BalI TGGCCA 1 cut(s) 907
BamHI GGATCC 1 cut(s) 646
BanI GGYRCC 1 cut(s) 1758
BanII GRGCYC 2 cut(s) 411, 1123
BauI CACGAG 4 cut(s) 441, 615, 626, 1529
BbsI GAAGAC 1 cut(s) 539
Bbv12I GWGCWC 3 cut(s) 32, 411, 1123
BbvI GCAGC 5 cut(s) 431, 598, 1571, 1951, 1954
BccI CCATC 3 cut(s) 916, 1003, 1052
BciT130I CCWGG 4 cut(s) 488, 673, 1769, 1823
BclI TGATCA 1 cut(s) 354
BcoDI GTCTC 6 cut(s) 629, 866, 966, 1356, 1458, 1486
BcuI ACTAGT 1 cut(s) 1240
BfaI CTAG 7 cut(s) 720, 1053, 1134, 1241, 1293, 1551, 1971
BfmI CTRYAG 2 cut(s) 336, 651
BfoI RGCGCY 1 cut(s) 47
BfrI CTTAAG 1 cut(s) 1511
BfuAI ACCTGC 1 cut(s) 1804
BglII AGATCT 1 cut(s) 1693
BisI GCNGC 7 cut(s) 8, 420, 612, 1275, 1560, 1965, 1968
BlsI GCNGC 7 cut(s) 9, 421, 613, 1276, 1561, 1966, 1969
Bme1390I CCNGG 4 cut(s) 488, 673, 1769, 1823
Bme18I GGWCC 3 cut(s) 522, 1064, 1816
BmgT120I GGNCC 3 cut(s) 522, 1064, 1816
BmiI GGNNCC 3 cut(s) 648, 1760, 1818
BmrFI CCNGG 4 cut(s) 488, 673, 1769, 1823
BmsI GCATC 6 cut(s) 406, 1106, 1219, 1313, 1578, 1920
BpiI GAAGAC 1 cut(s) 539
BpmI CTGGAG 1 cut(s) 1743
Bpu14I TTCGAA 1 cut(s) 1181
BpuEI CTTGAG 3 cut(s) 579, 1666, 1922
BsaJI CCNNGG 5 cut(s) 132, 487, 546, 672, 1821
BsaWI WCCGGW 2 cut(s) 248, 478
BsaXI ACNNNNNCTCC 2 cut(s) 1105, 1135
Bsc4I CCNNNNNNNGG 5 cut(s) 199, 464, 886, 1477, 1768
Bse118I RCCGGY 2 cut(s) 248, 478
Bse1I ACTGG 3 cut(s) 558, 1155, 1355
BseBI CCWGG 4 cut(s) 488, 673, 1769, 1823
BseDI CCNNGG 5 cut(s) 132, 487, 546, 672, 1821
BseGI GGATG 5 cut(s) 697, 995, 1063, 1261, 1328
BseLI CCNNNNNNNGG 5 cut(s) 199, 464, 886, 1477, 1768
BseMII CTCAG 5 cut(s) 24, 825, 1292, 1692, 1821
BseNI ACTGG 3 cut(s) 558, 1155, 1355
BseXI GCAGC 5 cut(s) 431, 598, 1571, 1951, 1954
BseYI CCCAGC 1 cut(s) 574
BshFI GGCC 3 cut(s) 734, 907, 1274
BshNI GGYRCC 1 cut(s) 1758
BshTI ACCGGT 2 cut(s) 248, 478
BsiHKAI GWGCWC 3 cut(s) 32, 411, 1123
BsiSI CCGG 2 cut(s) 249, 479
BslFI GGGAC 2 cut(s) 564, 1802
BslI CCNNNNNNNGG 5 cut(s) 199, 464, 886, 1477, 1768
BsmAI GTCTC 6 cut(s) 629, 866, 966, 1356, 1458, 1486
BsmBI CGTCTC 1 cut(s) 1356
BsmFI GGGAC 2 cut(s) 564, 1802
BsnI GGCC 3 cut(s) 734, 907, 1274
Bsp119I TTCGAA 1 cut(s) 1181
Bsp1286I GDGCHC 3 cut(s) 32, 411, 1123
Bsp143I GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
BspACI CCGC 6 cut(s) 7, 16, 644, 1275, 1348, 1501
BspANI GGCC 3 cut(s) 734, 907, 1274
BspCNI CTCAG 5 cut(s) 25, 826, 1291, 1693, 1822
BspLI GGNNCC 3 cut(s) 648, 1760, 1818
BspMI ACCTGC 1 cut(s) 1804
BspPI GGATC 4 cut(s) 641, 654, 1488, 1535
BspT104I TTCGAA 1 cut(s) 1181
BspT107I GGYRCC 1 cut(s) 1758
BspTI CTTAAG 1 cut(s) 1511
BsrBI CCGCTC 2 cut(s) 7, 1277
BsrFI RCCGGY 2 cut(s) 248, 478
BsrI ACTGG 3 cut(s) 558, 1155, 1355
BssAI RCCGGY 2 cut(s) 248, 478
BssECI CCNNGG 5 cut(s) 132, 487, 546, 672, 1821
BssMI GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
BssSI CACGAG 4 cut(s) 441, 615, 626, 1529
BssT1I CCWWGG 2 cut(s) 132, 546
Bst2BI CACGAG 4 cut(s) 441, 615, 626, 1529
Bst2UI CCWGG 4 cut(s) 488, 673, 1769, 1823
Bst4CI ACNGT 5 cut(s) 1290, 1336, 1472, 1783, 1870
Bst6I CTCTTC 3 cut(s) 901, 1391, 1920
BstAFI CTTAAG 1 cut(s) 1511
BstBI TTCGAA 1 cut(s) 1181
BstC8I GCNNGC 3 cut(s) 48, 1582, 1719
BstDEI CTNAG 7 cut(s) 33, 159, 834, 1278, 1701, 1830, 1934
BstEII GGTNACC 1 cut(s) 1350
BstF5I GGATG 5 cut(s) 697, 995, 1063, 1261, 1328
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 3 cut(s) 46, 1452, 1919
BstKTI GATC 6 cut(s) 357, 649, 1261, 1483, 1543, 1696
BstMAI GTCTC 6 cut(s) 629, 866, 966, 1356, 1458, 1486
BstMBI GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
BstMWI GCNNNNNNNGC 4 cut(s) 13, 425, 1914, 1939
BstNI CCWGG 4 cut(s) 488, 673, 1769, 1823
BstNSI RCATGY 2 cut(s) 61, 589
BstPI GGTNACC 1 cut(s) 1350
BstSCI CCNGG 4 cut(s) 486, 671, 1767, 1821
BstSFI CTRYAG 2 cut(s) 336, 651
BstV1I GCAGC 5 cut(s) 431, 598, 1571, 1951, 1954
BstV2I GAAGAC 1 cut(s) 539
BstX2I RGATCY 2 cut(s) 646, 1693
BstXI CCANNNNNNTGG 1 cut(s) 553
BstYI RGATCY 2 cut(s) 646, 1693
BsuRI GGCC 3 cut(s) 734, 907, 1274
BtgZI GCGATG 1 cut(s) 1923
BtsCI GGATG 5 cut(s) 697, 995, 1063, 1261, 1328
BtsIMutI CAGTG 2 cut(s) 865, 1792
BveI ACCTGC 1 cut(s) 1804
Cac8I GCNNGC 3 cut(s) 48, 1582, 1719
CfoI GCGC 3 cut(s) 46, 1452, 1919
Cfr10I RCCGGY 2 cut(s) 248, 478
Cfr13I GGNCC 3 cut(s) 522, 1064, 1816
Csp6I GTAC 6 cut(s) 55, 238, 482, 583, 1409, 1861
CspAI ACCGGT 2 cut(s) 248, 478
CspCI CAANNNNNGTGG 1 cut(s) 1976
CviAII CATG 5 cut(s) 58, 529, 586, 823, 1810
CviQI GTAC 6 cut(s) 55, 238, 482, 583, 1409, 1861
DdeI CTNAG 7 cut(s) 33, 159, 834, 1278, 1701, 1830, 1934
DpnI GATC 6 cut(s) 356, 648, 1260, 1482, 1542, 1695
DpnII GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
DraI TTTAAA 1 cut(s) 1681
EaeI YGGCCR 3 cut(s) 732, 905, 1272
Eam1104I CTCTTC 3 cut(s) 901, 1391, 1920
EarI CTCTTC 3 cut(s) 901, 1391, 1920
EciI GGCGGA 1 cut(s) 659
Ecl136II GAGCTC 2 cut(s) 409, 1121
Eco130I CCWWGG 2 cut(s) 132, 546
Eco24I GRGCYC 2 cut(s) 411, 1123
Eco32I GATATC 1 cut(s) 1284
Eco47I GGWCC 3 cut(s) 522, 1064, 1816
Eco47III AGCGCT 1 cut(s) 45
Eco53kI GAGCTC 2 cut(s) 409, 1121
Eco57I CTGAAG 3 cut(s) 444, 1415, 1689
Eco91I GGTNACC 1 cut(s) 1350
EcoICRI GAGCTC 2 cut(s) 409, 1121
EcoO109I RGGNCCY 2 cut(s) 1064, 1816
EcoO65I GGTNACC 1 cut(s) 1350
EcoRI GAATTC 1 cut(s) 331
EcoRII CCWGG 4 cut(s) 486, 671, 1767, 1821
EcoRV GATATC 1 cut(s) 1284
EcoT14I CCWWGG 2 cut(s) 132, 546
EcoT22I ATGCAT 1 cut(s) 1234
EcoT38I GRGCYC 2 cut(s) 411, 1123
ErhI CCWWGG 2 cut(s) 132, 546
Esp3I CGTCTC 1 cut(s) 1356
FaeI CATG 5 cut(s) 61, 532, 589, 826, 1813
FalI AAGNNNNNCTT 2 cut(s) 1917, 1949
FaqI GGGAC 2 cut(s) 564, 1802
FatI CATG 5 cut(s) 57, 528, 585, 822, 1809
FauNDI CATATG 3 cut(s) 343, 954, 1234
FbaI TGATCA 1 cut(s) 354
Fnu4HI GCNGC 7 cut(s) 8, 420, 612, 1275, 1560, 1965, 1968
FokI GGATG 5 cut(s) 704, 982, 1070, 1268, 1335
FriOI GRGCYC 2 cut(s) 411, 1123
Fsp4HI GCNGC 7 cut(s) 8, 420, 612, 1275, 1560, 1965, 1968
FspBI CTAG 7 cut(s) 720, 1053, 1134, 1241, 1293, 1551, 1971
GlaI GCGC 3 cut(s) 45, 1451, 1918
GluI GCNGC 7 cut(s) 8, 420, 612, 1275, 1560, 1965, 1968
GsaI CCCAGC 1 cut(s) 578
GsuI CTGGAG 1 cut(s) 1743
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 3 cut(s) 734, 907, 1274
HapII CCGG 2 cut(s) 249, 479
HhaI GCGC 3 cut(s) 46, 1452, 1919
Hin1II CATG 5 cut(s) 61, 532, 589, 826, 1813
Hin6I GCGC 3 cut(s) 44, 1450, 1917
HinP1I GCGC 3 cut(s) 44, 1450, 1917
HindIII AAGCTT 2 cut(s) 224, 1207
HinfI GANTC 4 cut(s) 102, 124, 163, 728
HpaII CCGG 2 cut(s) 249, 479
HphI GGTGA 3 cut(s) 1031, 1550, 1837
Hpy166II GTNNAC 7 cut(s) 401, 434, 484, 697, 816, 1110, 1508
Hpy8I GTNNAC 7 cut(s) 401, 434, 484, 697, 816, 1110, 1508
Hpy99I CGWCG 1 cut(s) 1117
HpyAV CCTTC 5 cut(s) 457, 593, 935, 1134, 1529
HpyCH4III ACNGT 5 cut(s) 1290, 1336, 1472, 1783, 1870
HpyCH4IV ACGT 1 cut(s) 1791
HpyCH4V TGCA 8 cut(s) 153, 232, 419, 528, 1097, 1232, 1326, 1813
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 425, 1914, 1939
HpyF3I CTNAG 7 cut(s) 33, 159, 834, 1278, 1701, 1830, 1934
HpySE526I ACGT 1 cut(s) 1791
Hsp92II CATG 5 cut(s) 61, 532, 589, 826, 1813
HspAI GCGC 3 cut(s) 44, 1450, 1917
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
LmnI GCTCC 5 cut(s) 4, 406, 1118, 1126, 1296
Lsp1109I GCAGC 5 cut(s) 431, 598, 1571, 1951, 1954
LweI GCATC 6 cut(s) 406, 1106, 1219, 1313, 1578, 1920
MaeI CTAG 7 cut(s) 720, 1053, 1134, 1241, 1293, 1551, 1971
MaeII ACGT 1 cut(s) 1791
MaeIII GTNAC 7 cut(s) 277, 348, 1350, 1472, 1787, 1825, 1946
MalI GATC 6 cut(s) 356, 648, 1260, 1482, 1542, 1695
MbiI CCGCTC 2 cut(s) 7, 1277
MboI GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
MboII GAAGA 8 cut(s) 284, 544, 888, 944, 974, 1238, 1408, 1937
MfeI CAATTG 2 cut(s) 783, 1268
MflI RGATCY 2 cut(s) 646, 1693
MhlI GDGCHC 3 cut(s) 32, 411, 1123
MlsI TGGCCA 1 cut(s) 907
MluNI TGGCCA 1 cut(s) 907
MlyI GAGTC 2 cut(s) 118, 722
MmeI TCCRAC 1 cut(s) 1339
Mox20I TGGCCA 1 cut(s) 907
Mph1103I ATGCAT 1 cut(s) 1234
MroXI GAANNNNTTC 2 cut(s) 1081, 1982
MscI TGGCCA 1 cut(s) 907
MseI TTAA 6 cut(s) 171, 1080, 1205, 1512, 1680, 1749
MslI CAYNNNNRTG 1 cut(s) 740
Msp20I TGGCCA 1 cut(s) 907
MspA1I CMGCKG 1 cut(s) 422
MspCI CTTAAG 1 cut(s) 1511
MspI CCGG 2 cut(s) 249, 479
MspR9I CCNGG 4 cut(s) 488, 673, 1769, 1823
MunI CAATTG 2 cut(s) 783, 1268
MvaI CCWGG 4 cut(s) 488, 673, 1769, 1823
MwoI GCNNNNNNNGC 4 cut(s) 13, 425, 1914, 1939
NdeI CATATG 3 cut(s) 343, 954, 1234
NdeII GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
NlaIII CATG 5 cut(s) 61, 532, 589, 826, 1813
NlaIV GGNNCC 3 cut(s) 648, 1760, 1818
NmeAIII GCCGAG 2 cut(s) 710, 1360
NmuCI GTSAC 2 cut(s) 1787, 1825
NsiI ATGCAT 1 cut(s) 1234
NspI RCATGY 2 cut(s) 61, 589
NspV TTCGAA 1 cut(s) 1181
PciI ACATGT 1 cut(s) 57
PdmI GAANNNNTTC 2 cut(s) 1081, 1982
PfeI GAWTC 2 cut(s) 102, 163
PflMI CCANNNNNTGG 1 cut(s) 1768
PinAI ACCGGT 2 cut(s) 248, 478
PkrI GCNGC 7 cut(s) 9, 421, 613, 1276, 1561, 1966, 1969
PleI GAGTC 2 cut(s) 118, 722
PpsI GAGTC 2 cut(s) 118, 722
PpuMI RGGWCCY 2 cut(s) 1064, 1816
PscI ACATGT 1 cut(s) 57
PshBI ATTAAT 1 cut(s) 1080
Psp124BI GAGCTC 2 cut(s) 411, 1123
Psp5II RGGWCCY 2 cut(s) 1064, 1816
Psp6I CCWGG 4 cut(s) 486, 671, 1767, 1821
PspEI GGTNACC 1 cut(s) 1350
PspFI CCCAGC 1 cut(s) 574
PspGI CCWGG 4 cut(s) 486, 671, 1767, 1821
PspN4I GGNNCC 3 cut(s) 648, 1760, 1818
PspPI GGNCC 3 cut(s) 522, 1064, 1816
PspPPI RGGWCCY 2 cut(s) 1064, 1816
PsuI RGATCY 2 cut(s) 646, 1693
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 6 cut(s) 56, 239, 483, 584, 1410, 1862
RsaNI GTAC 6 cut(s) 55, 238, 482, 583, 1409, 1861
RseI CAYNNNNRTG 1 cut(s) 740
SacI GAGCTC 2 cut(s) 411, 1123
SaqAI TTAA 6 cut(s) 171, 1080, 1205, 1512, 1680, 1749
SatI GCNGC 7 cut(s) 8, 420, 612, 1275, 1560, 1965, 1968
Sau3AI GATC 6 cut(s) 354, 646, 1258, 1480, 1540, 1693
Sau96I GGNCC 3 cut(s) 522, 1064, 1816
SchI GAGTC 2 cut(s) 118, 722
ScrFI CCNGG 4 cut(s) 488, 673, 1769, 1823
SduI GDGCHC 3 cut(s) 32, 411, 1123
SfaNI GCATC 6 cut(s) 406, 1106, 1219, 1313, 1578, 1920
SfcI CTRYAG 2 cut(s) 336, 651
SfuI TTCGAA 1 cut(s) 1181
SinI GGWCC 3 cut(s) 522, 1064, 1816
SmiMI CAYNNNNRTG 1 cut(s) 740
SmlI CTYRAG 4 cut(s) 594, 1511, 1645, 1901
SmoI CTYRAG 4 cut(s) 594, 1511, 1645, 1901
SpeI ACTAGT 1 cut(s) 1240
SsiI CCGC 6 cut(s) 7, 16, 644, 1275, 1348, 1501
SspI AATATT 1 cut(s) 1685
SspMI CTAG 7 cut(s) 720, 1053, 1134, 1241, 1293, 1551, 1971
SstI GAGCTC 2 cut(s) 411, 1123
StyD4I CCNGG 4 cut(s) 486, 671, 1767, 1821
StyI CCWWGG 2 cut(s) 132, 546
TaaI ACNGT 5 cut(s) 1290, 1336, 1472, 1783, 1870
TaiI ACGT 1 cut(s) 1794
TaqI TCGA 4 cut(s) 928, 1181, 1261, 1305
TatI WGTACW 3 cut(s) 54, 582, 1408
TauI GCSGC 2 cut(s) 10, 1277
TfiI GAWTC 2 cut(s) 102, 163
Tru1I TTAA 6 cut(s) 171, 1080, 1205, 1512, 1680, 1749
Tru9I TTAA 6 cut(s) 171, 1080, 1205, 1512, 1680, 1749
TscAI CASTG 2 cut(s) 865, 1792
TseFI GTSAC 2 cut(s) 1787, 1825
TseI GCWGC 5 cut(s) 419, 611, 1559, 1964, 1967
Tsp45I GTSAC 2 cut(s) 1787, 1825
TspDTI ATGAA 9 cut(s) 54, 402, 545, 860, 941, 971, 1116, 1251, 1758
TspGWI ACGGA 1 cut(s) 1380
TspRI CASTG 2 cut(s) 865, 1792
Van91I CCANNNNNTGG 1 cut(s) 1768
Vha464I CTTAAG 1 cut(s) 1511
VpaK11BI GGWCC 3 cut(s) 522, 1064, 1816
VspI ATTAAT 1 cut(s) 1080
XapI RAATTY 7 cut(s) 331, 389, 493, 1220, 1368, 1546, 1745
XceI RCATGY 2 cut(s) 61, 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 1849
XmnI GAANNNNTTC 2 cut(s) 1081, 1982
XspI CTAG 7 cut(s) 720, 1053, 1134, 1241, 1293, 1551, 1971
Zsp2I ATGCAT 1 cut(s) 1234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.