Rorug01G0028100

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002805
Physical Location & Seq
Forward (+)
4763714 .. 4774488
10775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0028100.1

Sequence Viewer

Length: 3300 bp
ATGGCGTGTATAAAGGGGGTGAACAGATCGGCGTCGGTGGCGGTCGCGCCGGACGCTCCGTACATGGCGGCCGGGACGATGGCGGGAGCGGTGGATCTGTCGTTCAGCTCCTCCGCGAATATCGAGATCTTCAAGCTCGATTTACAGTCGGACGATCGTGATCTTCCTGTAGTGGGAGAGTCCACGAGCTCCGAGAGATTCAACAGGTTATCGTGGTCCAAGCCCGCCGCTGGATCAGGCTCGCAAGACTTTGGCCTTGGGCTCATCGCCGGTGGACTCGTCGATGGGACTATTGATATCTGGAATCCGCTCACTCTGATCCGTTCTGAGACAGGGGAAAATGCTTCCGTTGAGCGTCTGACAAGACATAAGGGGCCTGTTCGTGGTCTTGAATTTAATGCAATCGCCCCAAATTTACTTGCATCTGGGGCCGACGATGGTGAAATTTGCATCTGGGACTTGGCTAATCCTACTGAGCCAACTCATTTTCCGCCTCTTAGGGGAAGTGGTTCTGCTGCCCAGGGTGAGATTTCATTTTTATCTTGGAATAGCAAGGTCCAACATATATTGGCATCCTCTTCGTATAATGGGACAACTGTGATTTGGGACCTAAAAAAGCAAAAGCCAGTGATAAGTTTTGCGGATTCAGTTAGAAGACGGTGCTCTGTTTTGCAGTGGAATCCCGATGTTGCCACTCAGCTTGTTGTTGCATCTGATGAAGATGGTTCACCTTCTCTCAGGCTCTGGGATATGCGTAATATATTGTCACCAGTTAAAGAGTTTGTGGGGCACACTAAAGGTGTAATTGCAATGTCATGGTGTCCTAATGACAGCTCTTATCTGCTAACGTGTGCGAAGGACAATCGAACTATTTGTTGGGACACAGTTTCTGCAGAGATTGTATGTGAATTGCCTGCAGGCACCAACTGGAATTTCGATGTGCATTGGTATCCGAAGGTACCTGGAGTGATATTAGCGTCTTCATTTGATGGAAAAATTGGAATTTATAATATTGAGGGTTGCAGTAGATATGGTGTGGGGGAGAGCGACTTTGGTGCTGGACCTTTCAGAGCTCCAAAATGGTATAAGCGCCCAGCTGGAGCATCTTTTGGTTTCGGAGGCAAGATCGTGTCCTTTCACCCTAGTTCATCTGGTGTTTCAGAGGTTTATGTGCACAGCTTGGTTACTGAACAAAGTTTGGTGGATCGCTCATCTGAATTTGAAAGTGCAATACAAAATGGGGAAAGGTCTTCACTCCGGGCTTTATGTGACAAGAAAGCACAGGAGTCTGAGTCTGCGGATGACCGGGAAACATGGGGCTTGTTAAGGGTTATGTTTGAAGATGATGGGACTGCAAGGACAAACCTGATCACACATCTTGGTTTCAGTGTACCTGAGGAAACAAAAGAAACAGTTCAAGATGGTCTATCAGCGGAAGTCAGTGCACCTGGGATTGAGGATTCAACAACTGATAAAGCTGGTTTGGATGCTGAGAAGGAAACTACAATTTTTCCTTCTGACAATGGAGAAGATTTCTTTAACAATCTTCCTAGTCCTAAAGCTGACACTCCTCATTCAACCTCTGGTGACAAATTTGTTGCGGCGGATACAGTTCCTATTACAGACCAAGTTCAAGAAGAACCTGATGAACCGGAAGAGAGCGCCGATCCTTCATTTGATGAATCAGTTCAACATGCTTTAGCTGTAGGAGATTATAAAGGGGCTGTTGCAAAGTGCATTTCTGCAAATAAAATGGCTGATGCTTTAGTTATTGCTCATGCTGGTGGTCCGACGTTGTGGGAGCGCACTCGGGATCAATACCTTAAGTTGAGCCATTCACCTTACCTTAAGATTGTTTCTGCAATGGTGAGCAATGATCTATCAAGTCTTGTTAACACCAGACCCCTCAAATTCTGGAAGGAAACACTTGCTGTTCTCTGTAGTTTTTCATCAATAGAGGCGTGGGAAGGTCTCTGTAATACGCTTGCTGCTAGACTTATAGCTGCTGGGAATACCCTGGCAGCAACTATTTGTTATATATGTGCGGGAAATATTGACAAGACAGTAGATATTTGGTCAAGGAATCTGACAACTGATCATGAAGGGAGATCCTATGTTGACCTTCTTCAGGAATTAATGGAGAAGACTATAGTACTTGCCTTGGCAACTGGACAGAAGCGATTTAGTGCTTCTTTGTGCAAACTTGTTGAGAAATATGCTGAAATTTTAGCTAGTCAAGGTCTTTTAACAACAGCAATGGAGTACCTGAAACTCTTGGGCTCTGATGAATTGTCTCCTGAACTTGTGATCTTAAGGGATCGTATTGCTCTTTCTACAGAACCCGTAGAGAAAGTCCCCAAGACTGTAACATTTGAAAACCCTGATCAAACCCCTTATGTGGGTGGGAACACTCCATATTATCAGGAACCAGCTCAGCCACCTGTGAATCCGTATGGCGGCTATTCTGAACCTGTGACTCCTCCGTATGCAGGTGGAGGATATCCTCATGTCCCATACCCCCAGCCTCCATCACAGACTCCATCACAGCCTCCTCACATGTTTGTTCCTACACCAACACCTCAACTACCACAGGAAAAATATCCTGTACCTCCTGCTTCTACTCAGCCTAACGTGTCCTTTATTCCTTCGACTCCTCCTACTCTGTCAAATGTGGAGAAGTATCATCAGCCTACGCTGGGTTCTCAATTGTATCCTGGAACTGCTTTTCAACCTATGCAAACAGGGCCTGGTTCAGGAGCCCCTCATCAATCACATGTCAATCCAGTTCCTGCAAATAAGATGCCTCATGCTGTACCCCCTCCACAGAGGGGATTTATGCCAGTCACTAATTCAGGAGTTGTTCAGGGGACTCTGCAGCCATCTAGTCCCCCTGCTCCACCTCGACCTTCCGTGGCTCCTGCTCCACCACCTACCATACAGACAGTTGATACTTCAAAAGTTCCTGGCCACCAAAAACCCGTAATCGCAACATTAGGGAGACTTTTCAATGAAACATCTGAAGCATTGGGAGGTTCACGTGCAAATCCTGCTAAGAAGCGTGAAATAGAAGACAATTCAAGGAAGATAGGTGCACTGTTTGCCAAACTCAACAGCGGAGACATATCTAAAAATGCTGGTGATAAACTTGTGCAGCTGTGCCAGGCACTGGATAATGGTGATTTCACCACTGCCCTAGGCATTCAGGTACAACTTACCACTAGTGAGTGGGATGAGTGCAACTTTTGGCTTGCAACTCTCAAGCGGATGATCAAGACCAGGCAAAATATGAGATTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

1099

Amino Acids

118.63

Weight (kDa)

5.17

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 88 - 252 6e-10 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 89 - 264 6.3e-13 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 89 - 157 5.3e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 89 - 229 1.5e-09 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 89 - 238 8.8e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2 PF23414 138 - 301 1e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 173 - 307 1.2e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 198 - 339 5.7e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 219 - 334 8.4e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 246 - 339 2.6e-09 WDR5 beta-propeller domain
TPR_Sec16 PF12931 563 - 758 6.1e-09 Sec16 Sec23-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1008, 1716
AarI CACCTGC 1 cut(s) 2480
Acc36I ACCTGC 1 cut(s) 2480
Acc65I GGTACC 1 cut(s) 958
AccB1I GGYRCC 2 cut(s) 920, 958
AccB7I CCANNNNNTGG 1 cut(s) 3166
AccBSI CCGCTC 2 cut(s) 89, 310
AccII CGCG 2 cut(s) 47, 116
AclWI GGATC 8 cut(s) 102, 241, 313, 1212, 1661, 1821, 2103, 2325
AcoI YGGCCR 2 cut(s) 69, 2965
AcsI RAATTY 9 cut(s) 392, 412, 444, 931, 1002, 1217, 1592, 1910, 2223
AcuI CTGAAG 2 cut(s) 2111, 3039
AcvI CACGTG 1 cut(s) 3038
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 8 cut(s) 62, 960, 1392, 2154, 2264, 2607, 2814, 3207
AflII CTTAAG 3 cut(s) 1823, 1847, 2311
AflIII ACRYGT 4 cut(s) 848, 2556, 2631, 2773
AhlI ACTAGT 1 cut(s) 3218
AjnI CCWGG 9 cut(s) 519, 961, 1447, 2016, 2713, 2746, 2962, 3159, 3275
AjuI GAANNNNNNNTTGG 4 cut(s) 859, 891, 917, 949
AloI GAACNNNNNNTCC 2 cut(s) 86, 118
Alw21I GWGCWC 6 cut(s) 191, 665, 1075, 1176, 1447, 3094
Alw26I GTCTC 5 cut(s) 323, 1976, 2298, 2992, 3111
Alw44I GTGCAC 3 cut(s) 1172, 1443, 3090
AlwI GGATC 8 cut(s) 102, 241, 313, 1212, 1661, 1821, 2103, 2325
AlwNI CAGNNNCTG 5 cut(s) 744, 890, 2747, 2789, 3166
Ama87I CYCGRG 1 cut(s) 1809
AoxI GGCC 6 cut(s) 69, 253, 374, 429, 2744, 2965
ApaLI GTGCAC 3 cut(s) 1172, 1443, 3090
ApeKI GCWGC 6 cut(s) 515, 1988, 2003, 2021, 2875, 3151
ApoI RAATTY 9 cut(s) 392, 412, 444, 931, 1002, 1217, 1592, 1910, 2223
AseI ATTAAT 1 cut(s) 2135
Asp700I GAANNNNTTC 3 cut(s) 508, 1413, 1686
Asp718I GGTACC 1 cut(s) 958
AspA2I CCTAGG 1 cut(s) 3193
AspLEI GCGC 4 cut(s) 49, 1092, 1664, 1806
AspS9I GGNCC 8 cut(s) 216, 374, 429, 556, 607, 1061, 1787, 2744
AsuC2I CCSGG 3 cut(s) 73, 1259, 1307
AvaI CYCGRG 1 cut(s) 1809
AvaII GGWCC 5 cut(s) 216, 556, 607, 1061, 1787
AvrII CCTAGG 1 cut(s) 3193
AxyI CCTNAGG 1 cut(s) 1395
BaeGI GKGCMC 4 cut(s) 792, 1176, 1447, 3094
BalI TGGCCA 1 cut(s) 2967
BanI GGYRCC 2 cut(s) 920, 958
BanII GRGCYC 5 cut(s) 191, 264, 1075, 2282, 2761
BauI CACGAG 1 cut(s) 184
BbrPI CACGTG 1 cut(s) 3038
BbsI GAAGAC 5 cut(s) 661, 972, 1242, 2150, 3075
Bbv12I GWGCWC 6 cut(s) 191, 665, 1075, 1176, 1447, 3094
BbvI GCAGC 6 cut(s) 502, 1975, 1990, 2033, 2887, 3163
BcgI CGANNNNNNTGC 4 cut(s) 561, 595, 1037, 1071
BciT130I CCWGG 9 cut(s) 521, 963, 1449, 2018, 2715, 2748, 2964, 3161, 3277
BciVI GTATCC 3 cut(s) 960, 1600, 2721
BclI TGATCA 4 cut(s) 1368, 2095, 2383, 3267
BcnI CCSGG 3 cut(s) 73, 1259, 1307
BcoDI GTCTC 5 cut(s) 323, 1976, 2298, 2992, 3111
BcuI ACTAGT 1 cut(s) 3218
BfaI CTAG 7 cut(s) 1143, 1551, 1992, 2232, 2883, 3194, 3219
BfmI CTRYAG 8 cut(s) 168, 891, 915, 1704, 1939, 2148, 2334, 2873
BfoI RGCGCY 2 cut(s) 1093, 1665
BfrI CTTAAG 3 cut(s) 1823, 1847, 2311
BfuAI ACCTGC 1 cut(s) 2480
BfuI GTATCC 3 cut(s) 960, 1600, 2721
BglII AGATCT 1 cut(s) 126
BlnI CCTAGG 1 cut(s) 3193
BlpI GCTNAGC 1 cut(s) 2433
BmcAI AGTACT 1 cut(s) 2154
Bme18I GGWCC 5 cut(s) 216, 556, 607, 1061, 1787
BmeT110I CYCGRG 1 cut(s) 1809
BmgT120I GGNCC 8 cut(s) 216, 374, 429, 556, 607, 1061, 1787, 2744
BmiI GGNNCC 8 cut(s) 375, 430, 608, 922, 960, 2427, 2758, 2916
BmsI GCATC 8 cut(s) 431, 459, 581, 719, 1112, 1477, 1750, 2790
BpiI GAAGAC 5 cut(s) 661, 972, 1242, 2150, 3075
BpmI CTGGAG 2 cut(s) 984, 1119
Bpu1102I GCTNAGC 1 cut(s) 2433
BpuEI CTTGAG 1 cut(s) 3242
BpuMI CCSGG 3 cut(s) 73, 1259, 1307
BsaAI YACGTR 1 cut(s) 3038
BsaBI GATNNNNATC 1 cut(s) 159
BsaHI GRCGYC 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 1976
BsaJI CCNNGG 8 cut(s) 256, 519, 520, 1448, 2016, 2160, 2910, 3193
BsaWI WCCGGW 1 cut(s) 1651
BsaXI ACNNNNNCTCC 2 cut(s) 1277, 1307
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 7 cut(s) 626, 770, 932, 2173, 2783, 2840, 3171
Bse21I CCTNAGG 1 cut(s) 1395
Bse3DI GCAATG 4 cut(s) 816, 1869, 1879, 2262
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 9 cut(s) 521, 963, 1449, 2018, 2715, 2748, 2964, 3161, 3277
BseDI CCNNGG 8 cut(s) 256, 519, 520, 1448, 2016, 2160, 2910, 3193
BseGI GGATG 5 cut(s) 572, 1306, 1492, 3235, 3270
BseJI GATNNNNATC 1 cut(s) 159
BseMI GCAATG 4 cut(s) 816, 1869, 1879, 2262
BseMII CTCAG 9 cut(s) 318, 465, 710, 751, 1281, 1386, 1482, 2447, 2636
BseNI ACTGG 7 cut(s) 626, 770, 932, 2173, 2783, 2840, 3171
BseRI GAGGAG 5 cut(s) 100, 1560, 2469, 2541, 2643
BseSI GKGCMC 4 cut(s) 792, 1176, 1447, 3094
BseX3I CGGCCG 1 cut(s) 69
BseXI GCAGC 6 cut(s) 502, 1975, 1990, 2033, 2887, 3163
BseYI CCCAGC 4 cut(s) 1093, 2006, 2520, 2695
BsgI GTGCAG 1 cut(s) 3170
Bsh1236I CGCG 2 cut(s) 47, 116
Bsh1285I CGRYCG 3 cut(s) 45, 72, 157
BshFI GGCC 6 cut(s) 71, 255, 376, 431, 2746, 2967
BshNI GGYRCC 2 cut(s) 920, 958
BsiEI CGRYCG 3 cut(s) 45, 72, 157
BsiHKAI GWGCWC 6 cut(s) 191, 665, 1075, 1176, 1447, 3094
BsiHKCI CYCGRG 1 cut(s) 1809
BsiSI CCGG 6 cut(s) 50, 72, 270, 1258, 1306, 1652
BsmAI GTCTC 5 cut(s) 323, 1976, 2298, 2992, 3111
BsmI GAATGC 1 cut(s) 3198
BsnI GGCC 6 cut(s) 71, 255, 376, 431, 2746, 2967
Bso31I GGTCTC 1 cut(s) 1976
BsoBI CYCGRG 1 cut(s) 1809
Bsp1720I GCTNAGC 1 cut(s) 2433
BspANI GGCC 6 cut(s) 71, 255, 376, 431, 2746, 2967
BspCNI CTCAG 9 cut(s) 319, 466, 709, 750, 1282, 1387, 1483, 2446, 2635
BspFNI CGCG 2 cut(s) 47, 116
BspHI TCATGA 1 cut(s) 2098
BspLI GGNNCC 8 cut(s) 375, 430, 608, 922, 960, 2427, 2758, 2916
BspMAI CTGCAG 3 cut(s) 895, 919, 2877
BspMI ACCTGC 1 cut(s) 2480
BspPI GGATC 8 cut(s) 102, 241, 313, 1212, 1661, 1821, 2103, 2325
BspT107I GGYRCC 2 cut(s) 920, 958
BspTI CTTAAG 3 cut(s) 1823, 1847, 2311
BspTNI GGTCTC 1 cut(s) 1976
BsrBI CCGCTC 2 cut(s) 89, 310
BsrDI GCAATG 4 cut(s) 816, 1869, 1879, 2262
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 7 cut(s) 626, 770, 932, 2173, 2783, 2840, 3171
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 8 cut(s) 256, 519, 520, 1448, 2016, 2160, 2910, 3193
BssNI GRCGYC 1 cut(s) 32
BssSI CACGAG 1 cut(s) 184
BssT1I CCWWGG 3 cut(s) 256, 2160, 3193
Bst2BI CACGAG 1 cut(s) 184
Bst2UI CCWGG 9 cut(s) 521, 963, 1449, 2018, 2715, 2748, 2964, 3161, 3277
Bst6I CTCTTC 2 cut(s) 583, 1650
BstACI GRCGYC 1 cut(s) 32
BstAFI CTTAAG 3 cut(s) 1823, 1847, 2311
BstAPI GCANNNNNTGC 2 cut(s) 3047, 3098
BstBAI YACGTR 1 cut(s) 3038
BstC8I GCNNGC 6 cut(s) 225, 242, 915, 919, 1986, 3249
BstDSI CCRYGG 1 cut(s) 2910
BstENI CCTNNNNNAGG 2 cut(s) 2126, 2751
BstF5I GGATG 5 cut(s) 572, 1306, 1492, 3235, 3270
BstFNI CGCG 2 cut(s) 47, 116
BstH2I RGCGCY 2 cut(s) 1093, 1665
BstHHI GCGC 4 cut(s) 49, 1092, 1664, 1806
BstMAI GTCTC 5 cut(s) 323, 1976, 2298, 2992, 3111
BstMCI CGRYCG 3 cut(s) 45, 72, 157
BstMWI GCNNNNNNNGC 6 cut(s) 38, 53, 428, 2743, 3047, 3098
BstNI CCWGG 9 cut(s) 521, 963, 1449, 2018, 2715, 2748, 2964, 3161, 3277
BstNSI RCATGY 3 cut(s) 1697, 2560, 2777
BstSFI CTRYAG 8 cut(s) 168, 891, 915, 1704, 1939, 2148, 2334, 2873
BstSLI GKGCMC 4 cut(s) 792, 1176, 1447, 3094
BstUI CGCG 2 cut(s) 47, 116
BstV1I GCAGC 6 cut(s) 502, 1975, 1990, 2033, 2887, 3163
BstV2I GAAGAC 5 cut(s) 661, 972, 1242, 2150, 3075
BstX2I RGATCY 3 cut(s) 94, 126, 2108
BstYI RGATCY 3 cut(s) 94, 126, 2108
BstZI CGGCCG 1 cut(s) 69
Bsu36I CCTNAGG 1 cut(s) 1395
BsuI GTATCC 3 cut(s) 960, 1600, 2721
BsuRI GGCC 6 cut(s) 71, 255, 376, 431, 2746, 2967
BtgI CCRYGG 1 cut(s) 2910
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 5 cut(s) 572, 1306, 1492, 3235, 3270
BtsI GCAGTG 2 cut(s) 680, 3186
BtsIMutI CAGTG 7 cut(s) 633, 680, 1393, 1447, 3092, 3164, 3186
BveI ACCTGC 1 cut(s) 2480
Cac8I GCNNGC 6 cut(s) 225, 242, 915, 919, 1986, 3249
CaiI CAGNNNCTG 5 cut(s) 744, 890, 2747, 2789, 3166
CciI TCATGA 1 cut(s) 2098
CfoI GCGC 4 cut(s) 49, 1092, 1664, 1806
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 8 cut(s) 216, 374, 429, 556, 607, 1061, 1787, 2744
CseI GACGC 4 cut(s) 21, 62, 344, 966
Csp6I GTAC 8 cut(s) 61, 959, 1391, 2153, 2263, 2606, 2813, 3206
CspCI CAANNNNNGTGG 2 cut(s) 682, 717
CviQI GTAC 8 cut(s) 61, 959, 1391, 2153, 2263, 2606, 2813, 3206
EaeI YGGCCR 2 cut(s) 69, 2965
EagI CGGCCG 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 583, 1650
EarI CTCTTC 2 cut(s) 583, 1650
EciI GGCGGA 2 cut(s) 480, 1619
Ecl136II GAGCTC 2 cut(s) 189, 1073
EclXI CGGCCG 1 cut(s) 69
Eco130I CCWWGG 3 cut(s) 256, 2160, 3193
Eco24I GRGCYC 5 cut(s) 191, 264, 1075, 2282, 2761
Eco31I GGTCTC 1 cut(s) 1976
Eco32I GATATC 2 cut(s) 298, 2501
Eco47I GGWCC 5 cut(s) 216, 556, 607, 1061, 1787
Eco52I CGGCCG 1 cut(s) 69
Eco53kI GAGCTC 2 cut(s) 189, 1073
Eco57I CTGAAG 2 cut(s) 2111, 3039
Eco72I CACGTG 1 cut(s) 3038
Eco81I CCTNAGG 1 cut(s) 1395
Eco88I CYCGRG 1 cut(s) 1809
EcoICRI GAGCTC 2 cut(s) 189, 1073
EcoNI CCTNNNNNAGG 2 cut(s) 2126, 2751
EcoO109I RGGNCCY 3 cut(s) 374, 607, 2744
EcoRII CCWGG 9 cut(s) 519, 961, 1447, 2016, 2713, 2746, 2962, 3159, 3275
EcoRV GATATC 2 cut(s) 298, 2501
EcoT14I CCWWGG 3 cut(s) 256, 2160, 3193
EcoT38I GRGCYC 5 cut(s) 191, 264, 1075, 2282, 2761
ErhI CCWWGG 3 cut(s) 256, 2160, 3193
FauI CCCGC 3 cut(s) 76, 232, 2038
FbaI TGATCA 4 cut(s) 1368, 2095, 2383, 3267
FokI GGATG 5 cut(s) 559, 1313, 1499, 3242, 3277
FriOI GRGCYC 5 cut(s) 191, 264, 1075, 2282, 2761
FspBI CTAG 7 cut(s) 1143, 1551, 1992, 2232, 2883, 3194, 3219
GlaI GCGC 4 cut(s) 48, 1091, 1663, 1805
GsaI CCCAGC 4 cut(s) 1097, 2010, 2524, 2699
GsuI CTGGAG 2 cut(s) 984, 1119
HaeII RGCGCY 2 cut(s) 1093, 1665
HaeIII GGCC 6 cut(s) 71, 255, 376, 431, 2746, 2967
HapII CCGG 6 cut(s) 50, 72, 270, 1258, 1306, 1652
HgaI GACGC 4 cut(s) 21, 62, 344, 966
HhaI GCGC 4 cut(s) 49, 1092, 1664, 1806
Hin1I GRCGYC 1 cut(s) 32
Hin6I GCGC 4 cut(s) 47, 1090, 1662, 1804
HinP1I GCGC 4 cut(s) 47, 1090, 1662, 1804
HincII GTYRAC 2 cut(s) 1894, 2119
HindII GTYRAC 2 cut(s) 1894, 2119
HpaI GTTAAC 1 cut(s) 1894
HpaII CCGG 6 cut(s) 50, 72, 270, 1258, 1306, 1652
Hpy99I CGWCG 4 cut(s) 37, 284, 437, 1795
HpyCH4IV ACGT 4 cut(s) 848, 1793, 2631, 3037
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 53, 428, 2743, 3047, 3098
HpySE526I ACGT 4 cut(s) 848, 1793, 2631, 3037
Hsp92I GRCGYC 1 cut(s) 32
HspAI GCGC 4 cut(s) 47, 1090, 1662, 1804
KpnI GGTACC 1 cut(s) 962
Ksp22I TGATCA 4 cut(s) 1368, 2095, 2383, 3267
KspAI GTTAAC 1 cut(s) 1894
Lsp1109I GCAGC 6 cut(s) 502, 1975, 1990, 2033, 2887, 3163
LweI GCATC 8 cut(s) 431, 459, 581, 719, 1112, 1477, 1750, 2790
MaeI CTAG 7 cut(s) 1143, 1551, 1992, 2232, 2883, 3194, 3219
MaeII ACGT 4 cut(s) 848, 1793, 2631, 3037
MaeIII GTNAC 7 cut(s) 765, 1183, 1268, 1586, 2365, 2473, 2842
MbiI CCGCTC 2 cut(s) 89, 310
MfeI CAATTG 1 cut(s) 2705
MflI RGATCY 3 cut(s) 94, 126, 2108
MlsI TGGCCA 1 cut(s) 2967
MluNI TGGCCA 1 cut(s) 2967
MlyI GAGTC 8 cut(s) 188, 270, 1295, 1301, 2470, 2530, 2644, 2863
MmeI TCCRAC 3 cut(s) 129, 583, 1814
Mox20I TGGCCA 1 cut(s) 2967
MroXI GAANNNNTTC 3 cut(s) 508, 1413, 1686
MscI TGGCCA 1 cut(s) 2967
MslI CAYNNNNRTG 1 cut(s) 1782
Msp20I TGGCCA 1 cut(s) 2967
MspA1I CMGCKG 5 cut(s) 230, 1097, 1433, 3114, 3154
MspCI CTTAAG 3 cut(s) 1823, 1847, 2311
MspI CCGG 6 cut(s) 50, 72, 270, 1258, 1306, 1652
MunI CAATTG 1 cut(s) 2705
Mva1269I GAATGC 1 cut(s) 3198
MvaI CCWGG 9 cut(s) 521, 963, 1449, 2018, 2715, 2748, 2964, 3161, 3277
MvnI CGCG 2 cut(s) 47, 116
MwoI GCNNNNNNNGC 6 cut(s) 38, 53, 428, 2743, 3047, 3098
NciI CCSGG 3 cut(s) 73, 1259, 1307
NlaIV GGNNCC 8 cut(s) 375, 430, 608, 922, 960, 2427, 2758, 2916
NmuCI GTSAC 5 cut(s) 765, 1268, 1586, 2473, 2842
NspI RCATGY 3 cut(s) 1697, 2560, 2777
PagI TCATGA 1 cut(s) 2098
PaqCI CACCTGC 1 cut(s) 2480
PasI CCCWGGG 1 cut(s) 520
PciI ACATGT 2 cut(s) 2556, 2773
PctI GAATGC 1 cut(s) 3198
PdmI GAANNNNTTC 3 cut(s) 508, 1413, 1686
PfeI GAWTC 8 cut(s) 198, 304, 644, 679, 1460, 1682, 2083, 2446
PflMI CCANNNNNTGG 1 cut(s) 3166
PfoI TCCNGGA 1 cut(s) 2713
Ple19I CGATCG 1 cut(s) 157
PleI GAGTC 8 cut(s) 187, 270, 1294, 1300, 2470, 2530, 2644, 2863
PmaCI CACGTG 1 cut(s) 3038
PmlI CACGTG 1 cut(s) 3038
PpsI GAGTC 8 cut(s) 187, 270, 1294, 1300, 2470, 2530, 2644, 2863
Ppu21I YACGTR 1 cut(s) 3038
PpuMI RGGWCCY 1 cut(s) 607
PscI ACATGT 2 cut(s) 2556, 2773
PshBI ATTAAT 1 cut(s) 2135
PsiI TTATAA 2 cut(s) 1008, 1716
Psp124BI GAGCTC 2 cut(s) 191, 1075
Psp5II RGGWCCY 1 cut(s) 607
Psp6I CCWGG 9 cut(s) 519, 961, 1447, 2016, 2713, 2746, 2962, 3159, 3275
PspCI CACGTG 1 cut(s) 3038
PspFI CCCAGC 4 cut(s) 1093, 2006, 2520, 2695
PspGI CCWGG 9 cut(s) 519, 961, 1447, 2016, 2713, 2746, 2962, 3159, 3275
PspN4I GGNNCC 8 cut(s) 375, 430, 608, 922, 960, 2427, 2758, 2916
PspPI GGNCC 8 cut(s) 216, 374, 429, 556, 607, 1061, 1787, 2744
PspPPI RGGWCCY 1 cut(s) 607
PstI CTGCAG 3 cut(s) 895, 919, 2877
PstNI CAGNNNCTG 5 cut(s) 744, 890, 2747, 2789, 3166
PsuI RGATCY 3 cut(s) 94, 126, 2108
PvuI CGATCG 1 cut(s) 157
PvuII CAGCTG 2 cut(s) 1097, 3154
RsaI GTAC 8 cut(s) 62, 960, 1392, 2154, 2264, 2607, 2814, 3207
RsaNI GTAC 8 cut(s) 61, 959, 1391, 2153, 2263, 2606, 2813, 3206
RseI CAYNNNNRTG 1 cut(s) 1782
SacI GAGCTC 2 cut(s) 191, 1075
Sau96I GGNCC 8 cut(s) 216, 374, 429, 556, 607, 1061, 1787, 2744
SbfI CCTGCAGG 1 cut(s) 919
ScaI AGTACT 1 cut(s) 2154
SchI GAGTC 8 cut(s) 188, 270, 1295, 1301, 2470, 2530, 2644, 2863
SdaI CCTGCAGG 1 cut(s) 919
SfaNI GCATC 8 cut(s) 431, 459, 581, 719, 1112, 1477, 1750, 2790
SfcI CTRYAG 8 cut(s) 168, 891, 915, 1704, 1939, 2148, 2334, 2873
SgrAI CRCCGGYG 1 cut(s) 269
SinI GGWCC 5 cut(s) 216, 556, 607, 1061, 1787
SmiMI CAYNNNNRTG 1 cut(s) 1782
SmlI CTYRAG 4 cut(s) 1823, 1847, 2311, 3257
SmoI CTYRAG 4 cut(s) 1823, 1847, 2311, 3257
SpeI ACTAGT 1 cut(s) 3218
Sse8387I CCTGCAGG 1 cut(s) 919
SspI AATATT 2 cut(s) 1012, 2053
SspMI CTAG 7 cut(s) 1143, 1551, 1992, 2232, 2883, 3194, 3219
SstI GAGCTC 2 cut(s) 191, 1075
StyI CCWWGG 3 cut(s) 256, 2160, 3193
TaiI ACGT 4 cut(s) 851, 1796, 2634, 3040
TaqI TCGA 7 cut(s) 123, 138, 282, 865, 936, 2648, 2902
TatI WGTACW 1 cut(s) 2152
TauI GCSGC 4 cut(s) 71, 230, 1604, 2460
TfiI GAWTC 8 cut(s) 198, 304, 644, 679, 1460, 1682, 2083, 2446
TscAI CASTG 7 cut(s) 633, 680, 1393, 1447, 3099, 3171, 3193
TseFI GTSAC 5 cut(s) 765, 1268, 1586, 2473, 2842
TseI GCWGC 6 cut(s) 515, 1988, 2003, 2021, 2875, 3151
Tsp45I GTSAC 5 cut(s) 765, 1268, 1586, 2473, 2842
TspGWI ACGGA 6 cut(s) 48, 311, 337, 2439, 2472, 2899
TspRI CASTG 7 cut(s) 633, 680, 1393, 1447, 3099, 3171, 3193
Van91I CCANNNNNTGG 1 cut(s) 3166
Vha464I CTTAAG 3 cut(s) 1823, 1847, 2311
VneI GTGCAC 3 cut(s) 1172, 1443, 3090
VpaK11BI GGWCC 5 cut(s) 216, 556, 607, 1061, 1787
VspI ATTAAT 1 cut(s) 2135
XagI CCTNNNNNAGG 2 cut(s) 2126, 2751
XapI RAATTY 9 cut(s) 392, 412, 444, 931, 1002, 1217, 1592, 1910, 2223
XceI RCATGY 3 cut(s) 1697, 2560, 2777
XmaJI CCTAGG 1 cut(s) 3193
XmnI GAANNNNTTC 3 cut(s) 508, 1413, 1686
XspI CTAG 7 cut(s) 1143, 1551, 1992, 2232, 2883, 3194, 3219
ZrmI AGTACT 1 cut(s) 2154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.