Rw1G003310

Crossover junction endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002805
Physical Location & Seq
Forward (+)
6097912 .. 6110192
12281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G003310.1

Sequence Viewer

Length: 2112 bp
ATGGAGCGGCACAAGCGGTTGGTGGTGTGCTCTGAGAATGAAGCACTGGCAGAGTACATGTTGAGTAAGTGGCAGGAAATGGCAGACAAGAAGCCCAAAGGAATCTCCGAAAACATTGAAAAGACTCTTACCAAGGCATACTCCAATGTCTGCAACTCTAAGAACCCTGTTAAAACCCTCAAAGAGTTTTCCCAAATCAAGGGTGTTGGGAAGTGGATTTTGAAGCTTATGCAAGGGTACTTTGACACCGGATCAGGCAGTTCTGAACCAGAAGATGTAACTAAAAAGGGTACGTCTTACATATTTCCTTGGCTTTACACTCTCTGTTTTCATGAATCGCATTGTACATTCCTTCTTGTTGCTGTCCATTATGGACTTGTTCTTTTATTAGGTAAGAGAACCAAAGGAAGCAAACGCTATGTTCCACAAAAGAATTCTGTAGCATATGCGTTACTGATCACACTTTACAGGGGAACTGAAAGTGGGAATGAATTTATGCGTAAACAGGAGCTCATTGATGCAGCTGAAGCAAGTGGACTATCTCGTGTTCCAATTATGCCAGAGAAGGGAAAGGGAATACCGGTACACCTGGGAAATTCTACAAGGGAATGGTATAGTGGTTGGTCCTGCATGAAGACATTGATAACCAAGGGACTGGTTGTGAAATCAAGTTGCCCAGCAAAGTACATGCTTACTCAAGAAGGTAAGGAGGCTGCTCGTGAATGTCTCGTGAGGTCTGGTTTGGCGGATCCTGTAGAAATAATTTCTAATGCCAGGGAGTTGTCTATCGGGGATGTGGACATTTCACATCAGGAACTTTCTAGTCCTGACTCGGCCACAGATATGACATTGTTATCCTGTAATTCAAGTAGGAATAAAAACTCAATTGATATTCCACAGGAATATCTTGACAAGTTTACACGCATGGGGTATTCTGAGGAACAGATGAACCGAGCTATCAGTGAAGTTTTAGAGACTTCTCATAACAAGGAAATCTCTTCACTATGGCCATCAGTTCTTTGTCGCCTTCGAGAAGATGAGGTTTATGGTTTTCATATGAAGTCTCAAAGCGTAAGGGGAAATCTTCGGGCAACACCATCCACTCATACTCTTGTGAATGGTGATAGAGATTGTGTTAGACTGCCCAACACTTCTAGGGATGGAGGTCCTACACCAAGAATTAATTCTGACAAGGTTGCATCAAAGTCGTTTACGACGGGAGCTCCTTCATCTCCTAGTTGTCCTGTGCCAAAGTCCAGTTTGGCTGGTGTTGAAGCAAGTTCGAAAGCTCTGTCTGTGCCACCTTTAAGCTTTGGGGAGAAATTTGAAGATGCATATGAACTAGTCTTTGTATTGGATGATCGAGAACAATTGGCTGCTCAGATATCACGGTCTAGGAGCATTATCGAGAGTGTTATTTCTGGATGCAAAATCACAGTAGAGGTTAGGCGGTTACCAGTTGGAGACGGAATTTGGATAGCTCGGCATAAACATCTGAAGAGTGAATATGTACTTGATTTTATTGTTGAGAGGAAAAATGTTGATGATTTGCGCTGTTCAATCAGAGACAACCGTTACAGGGATCAAAAATTGAGACTTTTGCGGTGTGGACTTAAGAAGCTGATATATCTCGTGGAAGGTGATCCAAATTCTAGTGAAGCAGCAGAAAGCATCAAAACAGCTTGCTTTACAACCGAGCTTTTGGAGGGCTTTGATGTTCAGAGAACGGGAAACCTGACTGAAACCTTGAGGAAGTATGGTTATCTTACTGAAGCAATTTTTAAATATTACAAAGATCTCTCTGAGGAACAGCATAAGCAAGCTGGAGTTTGTCCTCCTTTTGATGAATTTAACAAAAGGTGCCAAGACCTGGACAAGATGACAGTCAGTGACGTATTTGCCATTCAGCTCATGCAGGTCCCCCAGGTGACTGAGGAAGTTGCCATAGCTGTTCTGGATTTGTACCCAACAGTTTTTTCTCTTGCCCGTGAATACTCTCTACTTGAGGGCGATGTTGGCGCACAGGAAGAGATGCTTAGAACGCAGAGTAACAATGTTGTAAATGCTGCTGCTAGTAAGAATATATTCCACTTTGTTTGGGGCATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000280 GO:0000712 GO:0000724 GO:0000725 GO:0000727 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007093 GO:0007095 GO:0007127 GO:0007129 GO:0007131 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0016043 GO:0016787 GO:0016788 GO:0016889 GO:0016894 GO:0022402 GO:0022414 GO:0022607 GO:0031297 GO:0031570 GO:0031572 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044818 GO:0045005 GO:0045132 GO:0045143 GO:0045786 GO:0045930 GO:0046483 GO:0048256 GO:0048257 GO:0048285 GO:0048476 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0051726 GO:0061982 GO:0065007 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902749 GO:1902750 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko03440 ko03460 map03440 map03460
Pfam Domains
Protein Families
ERCC4

Protein Analysis

703

Amino Acids

78.81

Weight (kDa)

7.16

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_MUS81 PF21136 142 - 239 7.7e-12 Crossover junction endonuclease MUS81-like, winged helix domain
ERCC4 PF02732 473 - 583 2.3e-22 ERCC4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1906
AccB1I GGYRCC 1 cut(s) 1860
AccB7I CCANNNNNTGG 1 cut(s) 1870
AccBSI CCGCTC 1 cut(s) 7
AciI CCGC 5 cut(s) 7, 16, 746, 1450, 1603
AclWI GGATC 5 cut(s) 259, 743, 756, 1590, 1637
AcoI YGGCCR 2 cut(s) 834, 1007
AcsI RAATTY 7 cut(s) 433, 491, 595, 1322, 1470, 1648, 1847
AcuI CTGAAG 3 cut(s) 546, 1517, 1791
AfaI GTAC 8 cut(s) 56, 239, 292, 346, 585, 686, 1512, 1964
AfiI CCNNNNNNNGG 4 cut(s) 199, 566, 1579, 1870
AflII CTTAAG 1 cut(s) 1613
AflIII ACRYGT 1 cut(s) 57
AgeI ACCGGT 1 cut(s) 580
AgsI TTSAA 6 cut(s) 119, 223, 867, 1274, 1328, 1560
AhlI ACTAGT 1 cut(s) 1342
AjnI CCWGG 4 cut(s) 588, 773, 1869, 1923
AjuI GAANNNNNNNTTGG 4 cut(s) 1168, 1200, 1960, 1992
Alw21I GWGCWC 3 cut(s) 32, 513, 1225
Alw26I GTCTC 6 cut(s) 731, 968, 1068, 1458, 1560, 1588
AlwI GGATC 5 cut(s) 259, 743, 756, 1590, 1637
AoxI GGCC 2 cut(s) 834, 1007
ApeKI GCWGC 6 cut(s) 521, 713, 1376, 1661, 2066, 2069
ApoI RAATTY 7 cut(s) 433, 491, 595, 1322, 1470, 1648, 1847
ArsI GACNNNNNNTTYG 2 cut(s) 1277, 1309
AseI ATTAAT 1 cut(s) 1182
AsiGI ACCGGT 1 cut(s) 580
Asp700I GAANNNNTTC 2 cut(s) 1183, 2084
AspLEI GCGC 2 cut(s) 1554, 2021
AspS9I GGNCC 3 cut(s) 624, 1166, 1918
AsuHPI GGTGA 3 cut(s) 1133, 1652, 1939
AsuII TTCGAA 1 cut(s) 1283
AvaII GGWCC 3 cut(s) 624, 1166, 1918
BalI TGGCCA 1 cut(s) 1009
BamHI GGATCC 1 cut(s) 748
BanI GGYRCC 1 cut(s) 1860
BanII GRGCYC 2 cut(s) 513, 1225
BauI CACGAG 4 cut(s) 543, 717, 728, 1631
BbsI GAAGAC 1 cut(s) 641
Bbv12I GWGCWC 3 cut(s) 32, 513, 1225
BbvI GCAGC 6 cut(s) 533, 700, 1363, 1673, 2053, 2056
BccI CCATC 3 cut(s) 1018, 1105, 1154
BcgI CGANNNNNNTGC 2 cut(s) 1188, 1222
BciT130I CCWGG 4 cut(s) 590, 775, 1871, 1925
BclI TGATCA 1 cut(s) 456
BcoDI GTCTC 6 cut(s) 731, 968, 1068, 1458, 1560, 1588
BcuI ACTAGT 1 cut(s) 1342
BfaI CTAG 7 cut(s) 822, 1155, 1236, 1343, 1395, 1653, 2073
BfmI CTRYAG 2 cut(s) 438, 753
BfrI CTTAAG 1 cut(s) 1613
BfuAI ACCTGC 1 cut(s) 1906
BglII AGATCT 1 cut(s) 1795
BisI GCNGC 7 cut(s) 8, 522, 714, 1377, 1662, 2067, 2070
BlsI GCNGC 7 cut(s) 9, 523, 715, 1378, 1663, 2068, 2071
Bme1390I CCNGG 4 cut(s) 590, 775, 1871, 1925
Bme18I GGWCC 3 cut(s) 624, 1166, 1918
BmgT120I GGNCC 3 cut(s) 624, 1166, 1918
BmiI GGNNCC 3 cut(s) 750, 1862, 1920
BmrFI CCNGG 4 cut(s) 590, 775, 1871, 1925
BmsI GCATC 6 cut(s) 508, 1208, 1321, 1415, 1680, 2022
BpiI GAAGAC 1 cut(s) 641
BpmI CTGGAG 1 cut(s) 1845
Bpu14I TTCGAA 1 cut(s) 1283
BpuEI CTTGAG 3 cut(s) 681, 1768, 2024
BsaJI CCNNGG 6 cut(s) 132, 308, 589, 648, 774, 1923
BsaWI WCCGGW 2 cut(s) 248, 580
BsaXI ACNNNNNCTCC 2 cut(s) 1207, 1237
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 566, 1579, 1870
Bse118I RCCGGY 1 cut(s) 580
Bse1I ACTGG 4 cut(s) 51, 660, 1257, 1457
BseBI CCWGG 4 cut(s) 590, 775, 1871, 1925
BseDI CCNNGG 6 cut(s) 132, 308, 589, 648, 774, 1923
BseGI GGATG 5 cut(s) 799, 1097, 1165, 1363, 1430
BseLI CCNNNNNNNGG 4 cut(s) 199, 566, 1579, 1870
BseMII CTCAG 5 cut(s) 24, 927, 1394, 1794, 1923
BseNI ACTGG 4 cut(s) 51, 660, 1257, 1457
BseXI GCAGC 6 cut(s) 533, 700, 1363, 1673, 2053, 2056
BseYI CCCAGC 1 cut(s) 676
BshFI GGCC 2 cut(s) 836, 1009
BshNI GGYRCC 1 cut(s) 1860
BshTI ACCGGT 1 cut(s) 580
BsiHKAI GWGCWC 3 cut(s) 32, 513, 1225
BsiSI CCGG 2 cut(s) 249, 581
BslFI GGGAC 2 cut(s) 666, 1904
BslI CCNNNNNNNGG 4 cut(s) 199, 566, 1579, 1870
BsmAI GTCTC 6 cut(s) 731, 968, 1068, 1458, 1560, 1588
BsmBI CGTCTC 1 cut(s) 1458
BsmFI GGGAC 2 cut(s) 666, 1904
BsnI GGCC 2 cut(s) 836, 1009
Bsp119I TTCGAA 1 cut(s) 1283
Bsp1286I GDGCHC 3 cut(s) 32, 513, 1225
Bsp1407I TGTACA 1 cut(s) 344
Bsp143I GATC 7 cut(s) 251, 456, 748, 1360, 1582, 1642, 1795
BspACI CCGC 5 cut(s) 7, 16, 746, 1450, 1603
BspANI GGCC 2 cut(s) 836, 1009
BspCNI CTCAG 5 cut(s) 25, 928, 1393, 1795, 1924
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 3 cut(s) 750, 1862, 1920
BspMI ACCTGC 1 cut(s) 1906
BspPI GGATC 5 cut(s) 259, 743, 756, 1590, 1637
BspT104I TTCGAA 1 cut(s) 1283
BspT107I GGYRCC 1 cut(s) 1860
BspTI CTTAAG 1 cut(s) 1613
BsrBI CCGCTC 1 cut(s) 7
BsrFI RCCGGY 1 cut(s) 580
BsrGI TGTACA 1 cut(s) 344
BsrI ACTGG 4 cut(s) 51, 660, 1257, 1457
BssAI RCCGGY 1 cut(s) 580
BssECI CCNNGG 6 cut(s) 132, 308, 589, 648, 774, 1923
BssMI GATC 7 cut(s) 251, 456, 748, 1360, 1582, 1642, 1795
BssSI CACGAG 4 cut(s) 543, 717, 728, 1631
BssT1I CCWWGG 3 cut(s) 132, 308, 648
Bst2BI CACGAG 4 cut(s) 543, 717, 728, 1631
Bst2UI CCWGG 4 cut(s) 590, 775, 1871, 1925
Bst4CI ACNGT 5 cut(s) 1392, 1438, 1574, 1885, 1972
Bst6I CTCTTC 3 cut(s) 1003, 1493, 2022
BstAFI CTTAAG 1 cut(s) 1613
BstAUI TGTACA 1 cut(s) 344
BstBI TTCGAA 1 cut(s) 1283
BstC8I GCNNGC 2 cut(s) 1684, 1821
BstDEI CTNAG 7 cut(s) 33, 159, 936, 1380, 1803, 1932, 2036
BstEII GGTNACC 1 cut(s) 1452
BstF5I GGATG 5 cut(s) 799, 1097, 1165, 1363, 1430
BstHHI GCGC 2 cut(s) 1554, 2021
BstKTI GATC 7 cut(s) 254, 459, 751, 1363, 1585, 1645, 1798
BstMAI GTCTC 6 cut(s) 731, 968, 1068, 1458, 1560, 1588
BstMBI GATC 7 cut(s) 251, 456, 748, 1360, 1582, 1642, 1795
BstMWI GCNNNNNNNGC 4 cut(s) 13, 527, 2016, 2041
BstNI CCWGG 4 cut(s) 590, 775, 1871, 1925
BstNSI RCATGY 2 cut(s) 61, 691
BstPI GGTNACC 1 cut(s) 1452
BstSCI CCNGG 4 cut(s) 588, 773, 1869, 1923
BstSFI CTRYAG 2 cut(s) 438, 753
BstV1I GCAGC 6 cut(s) 533, 700, 1363, 1673, 2053, 2056
BstV2I GAAGAC 1 cut(s) 641
BstX2I RGATCY 2 cut(s) 748, 1795
BstXI CCANNNNNNTGG 1 cut(s) 655
BstYI RGATCY 2 cut(s) 748, 1795
BsuRI GGCC 2 cut(s) 836, 1009
BtgZI GCGATG 1 cut(s) 2025
BtsCI GGATG 5 cut(s) 799, 1097, 1165, 1363, 1430
BtsIMutI CAGTG 3 cut(s) 44, 967, 1894
BveI ACCTGC 1 cut(s) 1906
Cac8I GCNNGC 2 cut(s) 1684, 1821
CciI TCATGA 1 cut(s) 331
CfoI GCGC 2 cut(s) 1554, 2021
Cfr10I RCCGGY 1 cut(s) 580
Cfr13I GGNCC 3 cut(s) 624, 1166, 1918
Csp6I GTAC 8 cut(s) 55, 238, 291, 345, 584, 685, 1511, 1963
CspAI ACCGGT 1 cut(s) 580
CspCI CAANNNNNGTGG 1 cut(s) 2078
CviAII CATG 6 cut(s) 58, 332, 631, 688, 925, 1912
CviQI GTAC 8 cut(s) 55, 238, 291, 345, 584, 685, 1511, 1963
DdeI CTNAG 7 cut(s) 33, 159, 936, 1380, 1803, 1932, 2036
DpnI GATC 7 cut(s) 253, 458, 750, 1362, 1584, 1644, 1797
DpnII GATC 7 cut(s) 251, 456, 748, 1360, 1582, 1642, 1795
DraI TTTAAA 1 cut(s) 1783
EaeI YGGCCR 2 cut(s) 834, 1007
Eam1104I CTCTTC 3 cut(s) 1003, 1493, 2022
EarI CTCTTC 3 cut(s) 1003, 1493, 2022
EciI GGCGGA 1 cut(s) 761
Ecl136II GAGCTC 2 cut(s) 511, 1223
Eco130I CCWWGG 3 cut(s) 132, 308, 648
Eco24I GRGCYC 2 cut(s) 513, 1225
Eco32I GATATC 1 cut(s) 1386
Eco47I GGWCC 3 cut(s) 624, 1166, 1918
Eco53kI GAGCTC 2 cut(s) 511, 1223
Eco57I CTGAAG 3 cut(s) 546, 1517, 1791
Eco91I GGTNACC 1 cut(s) 1452
EcoICRI GAGCTC 2 cut(s) 511, 1223
EcoO109I RGGNCCY 2 cut(s) 1166, 1918
EcoO65I GGTNACC 1 cut(s) 1452
EcoRI GAATTC 1 cut(s) 433
EcoRII CCWGG 4 cut(s) 588, 773, 1869, 1923
EcoRV GATATC 1 cut(s) 1386
EcoT14I CCWWGG 3 cut(s) 132, 308, 648
EcoT22I ATGCAT 1 cut(s) 1336
EcoT38I GRGCYC 2 cut(s) 513, 1225
ErhI CCWWGG 3 cut(s) 132, 308, 648
Esp3I CGTCTC 1 cut(s) 1458
FaeI CATG 6 cut(s) 61, 335, 634, 691, 928, 1915
FalI AAGNNNNNCTT 2 cut(s) 2019, 2051
FaqI GGGAC 2 cut(s) 666, 1904
FatI CATG 6 cut(s) 57, 331, 630, 687, 924, 1911
FauNDI CATATG 3 cut(s) 445, 1056, 1336
FbaI TGATCA 1 cut(s) 456
Fnu4HI GCNGC 7 cut(s) 8, 522, 714, 1377, 1662, 2067, 2070
FokI GGATG 5 cut(s) 806, 1084, 1172, 1370, 1437
FriOI GRGCYC 2 cut(s) 513, 1225
Fsp4HI GCNGC 7 cut(s) 8, 522, 714, 1377, 1662, 2067, 2070
FspBI CTAG 7 cut(s) 822, 1155, 1236, 1343, 1395, 1653, 2073
GlaI GCGC 2 cut(s) 1553, 2020
GluI GCNGC 7 cut(s) 8, 522, 714, 1377, 1662, 2067, 2070
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 1 cut(s) 1845
HaeIII GGCC 2 cut(s) 836, 1009
HapII CCGG 2 cut(s) 249, 581
HhaI GCGC 2 cut(s) 1554, 2021
Hin1II CATG 6 cut(s) 61, 335, 634, 691, 928, 1915
Hin6I GCGC 2 cut(s) 1552, 2019
HinP1I GCGC 2 cut(s) 1552, 2019
HindIII AAGCTT 2 cut(s) 224, 1309
HinfI GANTC 4 cut(s) 102, 124, 335, 830
HpaII CCGG 2 cut(s) 249, 581
HphI GGTGA 3 cut(s) 1133, 1652, 1939
Hpy166II GTNNAC 7 cut(s) 503, 536, 586, 799, 918, 1212, 1610
Hpy8I GTNNAC 7 cut(s) 503, 536, 586, 799, 918, 1212, 1610
Hpy99I CGWCG 1 cut(s) 1219
HpyAV CCTTC 6 cut(s) 362, 559, 695, 1037, 1236, 1631
HpyCH4III ACNGT 5 cut(s) 1392, 1438, 1574, 1885, 1972
HpyCH4IV ACGT 2 cut(s) 293, 1893
HpyCH4V TGCA 8 cut(s) 153, 232, 521, 630, 1199, 1334, 1428, 1915
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 527, 2016, 2041
HpyF3I CTNAG 7 cut(s) 33, 159, 936, 1380, 1803, 1932, 2036
HpySE526I ACGT 2 cut(s) 293, 1893
Hsp92II CATG 6 cut(s) 61, 335, 634, 691, 928, 1915
HspAI GCGC 2 cut(s) 1552, 2019
Ksp22I TGATCA 1 cut(s) 456
Kzo9I GATC 7 cut(s) 251, 456, 748, 1360, 1582, 1642, 1795
LmnI GCTCC 5 cut(s) 4, 508, 1220, 1228, 1398
Lsp1109I GCAGC 6 cut(s) 533, 700, 1363, 1673, 2053, 2056
LweI GCATC 6 cut(s) 508, 1208, 1321, 1415, 1680, 2022
MaeI CTAG 7 cut(s) 822, 1155, 1236, 1343, 1395, 1653, 2073
MaeII ACGT 2 cut(s) 293, 1893
MaeIII GTNAC 7 cut(s) 277, 450, 1452, 1574, 1889, 1927, 2048
MalI GATC 7 cut(s) 253, 458, 750, 1362, 1584, 1644, 1797
MbiI CCGCTC 1 cut(s) 7
MboI GATC 7 cut(s) 251, 456, 748, 1360, 1582, 1642, 1795
MboII GAAGA 8 cut(s) 284, 646, 990, 1046, 1076, 1340, 1510, 2039
MfeI CAATTG 2 cut(s) 885, 1370
MflI RGATCY 2 cut(s) 748, 1795
MhlI GDGCHC 3 cut(s) 32, 513, 1225
MlsI TGGCCA 1 cut(s) 1009
MluNI TGGCCA 1 cut(s) 1009
MlyI GAGTC 2 cut(s) 118, 824
MmeI TCCRAC 1 cut(s) 1441
Mox20I TGGCCA 1 cut(s) 1009
Mph1103I ATGCAT 1 cut(s) 1336
MroXI GAANNNNTTC 2 cut(s) 1183, 2084
MscI TGGCCA 1 cut(s) 1009
MseI TTAA 6 cut(s) 171, 1182, 1307, 1614, 1782, 1851
MslI CAYNNNNRTG 1 cut(s) 842
Msp20I TGGCCA 1 cut(s) 1009
MspA1I CMGCKG 1 cut(s) 524
MspCI CTTAAG 1 cut(s) 1613
MspI CCGG 2 cut(s) 249, 581
MspR9I CCNGG 4 cut(s) 590, 775, 1871, 1925
MunI CAATTG 2 cut(s) 885, 1370
MvaI CCWGG 4 cut(s) 590, 775, 1871, 1925
MwoI GCNNNNNNNGC 4 cut(s) 13, 527, 2016, 2041
NdeI CATATG 3 cut(s) 445, 1056, 1336
NdeII GATC 7 cut(s) 251, 456, 748, 1360, 1582, 1642, 1795
NlaIII CATG 6 cut(s) 61, 335, 634, 691, 928, 1915
NlaIV GGNNCC 3 cut(s) 750, 1862, 1920
NmeAIII GCCGAG 2 cut(s) 812, 1462
NmuCI GTSAC 2 cut(s) 1889, 1927
NsiI ATGCAT 1 cut(s) 1336
NspI RCATGY 2 cut(s) 61, 691
NspV TTCGAA 1 cut(s) 1283
PagI TCATGA 1 cut(s) 331
PciI ACATGT 1 cut(s) 57
PdmI GAANNNNTTC 2 cut(s) 1183, 2084
PfeI GAWTC 2 cut(s) 102, 335
PflMI CCANNNNNTGG 1 cut(s) 1870
PinAI ACCGGT 1 cut(s) 580
PkrI GCNGC 7 cut(s) 9, 523, 715, 1378, 1663, 2068, 2071
PleI GAGTC 2 cut(s) 118, 824
PpsI GAGTC 2 cut(s) 118, 824
PpuMI RGGWCCY 2 cut(s) 1166, 1918
PscI ACATGT 1 cut(s) 57
PshBI ATTAAT 1 cut(s) 1182
Psp124BI GAGCTC 2 cut(s) 513, 1225
Psp5II RGGWCCY 2 cut(s) 1166, 1918
Psp6I CCWGG 4 cut(s) 588, 773, 1869, 1923
PspEI GGTNACC 1 cut(s) 1452
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 4 cut(s) 588, 773, 1869, 1923
PspN4I GGNNCC 3 cut(s) 750, 1862, 1920
PspPI GGNCC 3 cut(s) 624, 1166, 1918
PspPPI RGGWCCY 2 cut(s) 1166, 1918
PsuI RGATCY 2 cut(s) 748, 1795
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 8 cut(s) 56, 239, 292, 346, 585, 686, 1512, 1964
RsaNI GTAC 8 cut(s) 55, 238, 291, 345, 584, 685, 1511, 1963
RseI CAYNNNNRTG 1 cut(s) 842
SacI GAGCTC 2 cut(s) 513, 1225
SaqAI TTAA 6 cut(s) 171, 1182, 1307, 1614, 1782, 1851
SatI GCNGC 7 cut(s) 8, 522, 714, 1377, 1662, 2067, 2070
Sau3AI GATC 7 cut(s) 251, 456, 748, 1360, 1582, 1642, 1795
Sau96I GGNCC 3 cut(s) 624, 1166, 1918
SchI GAGTC 2 cut(s) 118, 824
ScrFI CCNGG 4 cut(s) 590, 775, 1871, 1925
SduI GDGCHC 3 cut(s) 32, 513, 1225
SfaNI GCATC 6 cut(s) 508, 1208, 1321, 1415, 1680, 2022
SfcI CTRYAG 2 cut(s) 438, 753
SfuI TTCGAA 1 cut(s) 1283
SinI GGWCC 3 cut(s) 624, 1166, 1918
SmiMI CAYNNNNRTG 1 cut(s) 842
SmlI CTYRAG 4 cut(s) 696, 1613, 1747, 2003
SmoI CTYRAG 4 cut(s) 696, 1613, 1747, 2003
SpeI ACTAGT 1 cut(s) 1342
SsiI CCGC 5 cut(s) 7, 16, 746, 1450, 1603
SspI AATATT 1 cut(s) 1787
SspMI CTAG 7 cut(s) 822, 1155, 1236, 1343, 1395, 1653, 2073
SstI GAGCTC 2 cut(s) 513, 1225
StyD4I CCNGG 4 cut(s) 588, 773, 1869, 1923
StyI CCWWGG 3 cut(s) 132, 308, 648
TaaI ACNGT 5 cut(s) 1392, 1438, 1574, 1885, 1972
TaiI ACGT 2 cut(s) 296, 1896
TaqI TCGA 4 cut(s) 1030, 1283, 1363, 1407
TatI WGTACW 4 cut(s) 54, 344, 684, 1510
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 2 cut(s) 102, 335
Tru1I TTAA 6 cut(s) 171, 1182, 1307, 1614, 1782, 1851
Tru9I TTAA 6 cut(s) 171, 1182, 1307, 1614, 1782, 1851
TscAI CASTG 3 cut(s) 51, 967, 1894
TseFI GTSAC 2 cut(s) 1889, 1927
TseI GCWGC 6 cut(s) 521, 713, 1376, 1661, 2066, 2069
Tsp45I GTSAC 2 cut(s) 1889, 1927
TspGWI ACGGA 1 cut(s) 1482
TspRI CASTG 3 cut(s) 51, 967, 1894
Van91I CCANNNNNTGG 1 cut(s) 1870
Vha464I CTTAAG 1 cut(s) 1613
VpaK11BI GGWCC 3 cut(s) 624, 1166, 1918
VspI ATTAAT 1 cut(s) 1182
XapI RAATTY 7 cut(s) 433, 491, 595, 1322, 1470, 1648, 1847
XceI RCATGY 2 cut(s) 61, 691
XcmI CCANNNNNNNNNTGG 1 cut(s) 1951
XmnI GAANNNNTTC 2 cut(s) 1183, 2084
XspI CTAG 7 cut(s) 822, 1155, 1236, 1343, 1395, 1653, 2073
Zsp2I ATGCAT 1 cut(s) 1336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.