AT4G35710

Arabidopsis protein of unknown function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004261
Physical Location & Seq
Forward (+)
16925301 .. 16926152
852 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35710.1

Sequence Viewer

Length: 852 bp
ATGGCGAATATGATCATTAAGAAACAGTTAAGATCTATAAGTTTACCTTCTAGATCACAACCAAGCACAAGTGGGCTCGAAGAATCTCTTAATAAGATTAAAACAATTAACACCACGACGGGTTCATCCGAGTCGATCTTGATGGGCTTGGCTGGTTTGGAGGAGCTCTACATTTTTCTGGAGGAGTTTCTGAAAATGGGTTCAAAGCAACGGGTTATGTCCTCTGGTGGGTCGGAATTCATGGAGGAAATGCTTGATGGTTCGTTGAGGCTGATGGATATCTGCAGCGTCTCAAGGGATCTAATGGTGGAGACTCACGAGCATGTTCGTGGCGTTCAGTCATATGTTAGAAGAAAGAAAGTATCTGGTGGAGGAGGAGGAGATAAAATCGATGTCGCTGTCTCCGACTATGTCGGATTTAGGAAGAACATGAGAAAAGAAGCCAAGAAGCTTCTAGGATCCTTGAAGAAAGTCGATGGAGGGACTCGTTCATGTGATAATGATCATGAAGATGAACAACTTGTGGCGGTTATAGATAGGGTGAGACGAGTGGTATCGGTAAGTGTCGTCGTATTGAAGTCGTTCTTGGAATTATTGTCCAGACGAAAAAGTAACATAAAGAGCAAGTTGGCGTCGGTACTAAAGATGAAGAAGGATAACCATGCGCCAGCCAAGAACGTGTTGGAGACATTAGATTCCGCGATTTTCGGAGATTTTTTATCTCATGATGATCTTCAAAATGAGCTTGAAGAGGTTGAGATGTGCATTGGTGGGTTCGAGAGAAATCTAGAAGGGTTGTTTCGAAGGTTGATAAGAACACGAGCCTCGATTCTCAACATAATCTCCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF241

Protein Analysis

283

Amino Acids

31.58

Weight (kDa)

9.02

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BPS1 PF03087 49 - 280 1.4e-62 Protein BPS1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 701
AciI CCGC 2 cut(s) 527, 699
AclWI GGATC 3 cut(s) 306, 453, 466
AcsI RAATTY 1 cut(s) 236
AcyI GRCGYC 1 cut(s) 632
AfaI GTAC 1 cut(s) 639
AfiI CCNNNNNNNGG 1 cut(s) 228
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 5 cut(s) 204, 466, 577, 737, 749
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AluBI AGCT 3 cut(s) 166, 451, 745
AluI AGCT 3 cut(s) 166, 451, 745
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 5 cut(s) 295, 305, 406, 538, 680
AlwI GGATC 3 cut(s) 306, 453, 466
ApeKI GCWGC 1 cut(s) 285
ApoI RAATTY 1 cut(s) 236
Asp700I GAANNNNTTC 1 cut(s) 581
AspLEI GCGC 1 cut(s) 667
AsuHPI GGTGA 1 cut(s) 553
AsuII TTCGAA 1 cut(s) 802
BamHI GGATCC 1 cut(s) 458
BanII GRGCYC 2 cut(s) 78, 168
BauI CACGAG 2 cut(s) 317, 819
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 297
BccI CCATC 4 cut(s) 136, 251, 268, 470
BclI TGATCA 2 cut(s) 12, 502
BcoDI GTCTC 5 cut(s) 295, 305, 406, 538, 680
BfaI CTAG 3 cut(s) 51, 455, 788
BfmI CTRYAG 1 cut(s) 283
BglII AGATCT 1 cut(s) 32
BisI GCNGC 1 cut(s) 286
BlsI GCNGC 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 460
BpmI CTGGAG 1 cut(s) 200
Bpu14I TTCGAA 1 cut(s) 802
BpuEI CTTGAG 1 cut(s) 277
Bsa29I ATCGAT 1 cut(s) 390
BsaBI GATNNNNATC 2 cut(s) 278, 501
BsaHI GRCGYC 1 cut(s) 632
BsaXI ACNNNNNCTCC 1 cut(s) 827
Bsc4I CCNNNNNNNGG 1 cut(s) 228
Bse8I GATNNNNATC 2 cut(s) 278, 501
BseCI ATCGAT 1 cut(s) 390
BseGI GGATG 1 cut(s) 125
BseJI GATNNNNATC 2 cut(s) 278, 501
BseLI CCNNNNNNNGG 1 cut(s) 228
BseRI GAGGAG 5 cut(s) 176, 197, 387, 390, 393
BseXI GCAGC 1 cut(s) 297
Bsh1236I CGCG 1 cut(s) 701
BshVI ATCGAT 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 168
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 1 cut(s) 228
BsmAI GTCTC 5 cut(s) 295, 305, 406, 538, 680
BsmBI CGTCTC 2 cut(s) 295, 538
BsmFI GGGAC 1 cut(s) 496
Bsp119I TTCGAA 1 cut(s) 802
Bsp1286I GDGCHC 2 cut(s) 78, 168
Bsp143I GATC 8 cut(s) 12, 32, 53, 135, 298, 458, 502, 730
BspACI CCGC 2 cut(s) 527, 699
BspDI ATCGAT 1 cut(s) 390
BspFNI CGCG 1 cut(s) 701
BspHI TCATGA 2 cut(s) 505, 724
BspLI GGNNCC 1 cut(s) 460
BspMAI CTGCAG 1 cut(s) 287
BspPI GGATC 3 cut(s) 306, 453, 466
BspT104I TTCGAA 1 cut(s) 802
BssMI GATC 8 cut(s) 12, 32, 53, 135, 298, 458, 502, 730
BssNI GRCGYC 1 cut(s) 632
BssSI CACGAG 2 cut(s) 317, 819
Bst2BI CACGAG 2 cut(s) 317, 819
Bst4CI ACNGT 1 cut(s) 27
Bst6I CTCTTC 1 cut(s) 744
BstACI GRCGYC 1 cut(s) 632
BstBI TTCGAA 1 cut(s) 802
BstC8I GCNNGC 1 cut(s) 669
BstF5I GGATG 1 cut(s) 125
BstFNI CGCG 1 cut(s) 701
BstHHI GCGC 1 cut(s) 667
BstKTI GATC 8 cut(s) 15, 35, 56, 138, 301, 461, 505, 733
BstMAI GTCTC 5 cut(s) 295, 305, 406, 538, 680
BstMBI GATC 8 cut(s) 12, 32, 53, 135, 298, 458, 502, 730
BstNSI RCATGY 1 cut(s) 326
BstSFI CTRYAG 1 cut(s) 283
BstUI CGCG 1 cut(s) 701
BstV1I GCAGC 1 cut(s) 297
BstX2I RGATCY 3 cut(s) 32, 298, 458
BstYI RGATCY 3 cut(s) 32, 298, 458
Bsu15I ATCGAT 1 cut(s) 390
BsuTUI ATCGAT 1 cut(s) 390
BtsCI GGATG 1 cut(s) 125
Cac8I GCNNGC 1 cut(s) 669
CciI TCATGA 2 cut(s) 505, 724
CfoI GCGC 1 cut(s) 667
ClaI ATCGAT 1 cut(s) 390
CseI GACGC 2 cut(s) 277, 621
Csp6I GTAC 1 cut(s) 638
CviAII CATG 7 cut(s) 241, 323, 430, 492, 506, 662, 725
CviQI GTAC 1 cut(s) 638
DpnI GATC 8 cut(s) 14, 34, 55, 137, 300, 460, 504, 732
DpnII GATC 8 cut(s) 12, 32, 53, 135, 298, 458, 502, 730
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
Ecl136II GAGCTC 1 cut(s) 166
Eco24I GRGCYC 2 cut(s) 78, 168
Eco32I GATATC 1 cut(s) 280
Eco53kI GAGCTC 1 cut(s) 166
EcoICRI GAGCTC 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 236
EcoRV GATATC 1 cut(s) 280
EcoT38I GRGCYC 2 cut(s) 78, 168
Esp3I CGTCTC 2 cut(s) 295, 538
FaeI CATG 7 cut(s) 244, 326, 433, 495, 509, 665, 728
FalI AAGNNNNNCTT 6 cut(s) 31, 63, 72, 104, 569, 601
FaqI GGGAC 1 cut(s) 496
FatI CATG 7 cut(s) 240, 322, 429, 491, 505, 661, 724
FauNDI CATATG 1 cut(s) 343
FbaI TGATCA 2 cut(s) 12, 502
Fnu4HI GCNGC 1 cut(s) 286
FokI GGATG 1 cut(s) 112
FriOI GRGCYC 2 cut(s) 78, 168
Fsp4HI GCNGC 1 cut(s) 286
FspBI CTAG 3 cut(s) 51, 455, 788
GlaI GCGC 1 cut(s) 666
GluI GCNGC 1 cut(s) 286
GsuI CTGGAG 1 cut(s) 200
HgaI GACGC 2 cut(s) 277, 621
HhaI GCGC 1 cut(s) 667
Hin1I GRCGYC 1 cut(s) 632
Hin1II CATG 7 cut(s) 244, 326, 433, 495, 509, 665, 728
Hin6I GCGC 1 cut(s) 665
HinP1I GCGC 1 cut(s) 665
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 6 cut(s) 83, 131, 313, 484, 695, 829
HphI GGTGA 1 cut(s) 553
Hpy166II GTNNAC 1 cut(s) 44
Hpy188I TCNGA 6 cut(s) 130, 192, 235, 406, 416, 710
Hpy188III TCNNGA 9 cut(s) 51, 139, 179, 317, 506, 600, 725, 778, 788
Hpy8I GTNNAC 1 cut(s) 44
Hpy99I CGWCG 3 cut(s) 121, 572, 637
HpyAV CCTTC 4 cut(s) 57, 646, 785, 798
HpyCH4III ACNGT 1 cut(s) 27
HpyCH4IV ACGT 1 cut(s) 678
HpyCH4V TGCA 2 cut(s) 285, 765
HpySE526I ACGT 1 cut(s) 678
Hsp92I GRCGYC 1 cut(s) 632
Hsp92II CATG 7 cut(s) 244, 326, 433, 495, 509, 665, 728
HspAI GCGC 1 cut(s) 665
Ksp22I TGATCA 2 cut(s) 12, 502
Kzo9I GATC 8 cut(s) 12, 32, 53, 135, 298, 458, 502, 730
LmnI GCTCC 1 cut(s) 163
LpnPI CCDG 6 cut(s) 138, 164, 210, 351, 613, 681
Lsp1109I GCAGC 1 cut(s) 297
MaeI CTAG 3 cut(s) 51, 455, 788
MaeII ACGT 1 cut(s) 678
MaeIII GTNAC 1 cut(s) 611
MalI GATC 8 cut(s) 14, 34, 55, 137, 300, 460, 504, 732
MboI GATC 8 cut(s) 12, 32, 53, 135, 298, 458, 502, 730
MboII GAAGA 8 cut(s) 92, 363, 436, 478, 521, 661, 725, 761
MflI RGATCY 3 cut(s) 32, 298, 458
MhlI GDGCHC 2 cut(s) 78, 168
MluCI AATT 3 cut(s) 105, 236, 590
MlyI GAGTC 3 cut(s) 140, 307, 478
MmeI TCCRAC 4 cut(s) 213, 394, 429, 663
MroXI GAANNNNTTC 1 cut(s) 581
MseI TTAA 5 cut(s) 18, 29, 90, 99, 108
MslI CAYNNNNRTG 3 cut(s) 321, 327, 510
MvnI CGCG 1 cut(s) 701
NdeI CATATG 1 cut(s) 343
NdeII GATC 8 cut(s) 12, 32, 53, 135, 298, 458, 502, 730
NlaIII CATG 7 cut(s) 244, 326, 433, 495, 509, 665, 728
NlaIV GGNNCC 1 cut(s) 460
NspI RCATGY 1 cut(s) 326
NspV TTCGAA 1 cut(s) 802
PagI TCATGA 2 cut(s) 505, 724
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PdmI GAANNNNTTC 1 cut(s) 581
PfeI GAWTC 3 cut(s) 83, 695, 829
PflFI GACNNNGTC 1 cut(s) 410
PkrI GCNGC 1 cut(s) 287
PleI GAGTC 3 cut(s) 139, 307, 478
PpsI GAGTC 3 cut(s) 139, 307, 478
Psp124BI GAGCTC 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 460
PstI CTGCAG 1 cut(s) 287
PsuI RGATCY 3 cut(s) 32, 298, 458
PsyI GACNNNGTC 1 cut(s) 410
RsaI GTAC 1 cut(s) 639
RsaNI GTAC 1 cut(s) 638
RseI CAYNNNNRTG 3 cut(s) 321, 327, 510
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 5 cut(s) 18, 29, 90, 99, 108
SatI GCNGC 1 cut(s) 286
Sau3AI GATC 8 cut(s) 12, 32, 53, 135, 298, 458, 502, 730
SchI GAGTC 3 cut(s) 140, 307, 478
SduI GDGCHC 2 cut(s) 78, 168
SetI ASST 7 cut(s) 49, 168, 453, 681, 747, 756, 809
SfcI CTRYAG 1 cut(s) 283
SfuI TTCGAA 1 cut(s) 802
SmiMI CAYNNNNRTG 3 cut(s) 321, 327, 510
SmlI CTYRAG 1 cut(s) 292
SmoI CTYRAG 1 cut(s) 292
Sse9I AATT 3 cut(s) 105, 236, 590
SsiI CCGC 2 cut(s) 527, 699
SspMI CTAG 3 cut(s) 51, 455, 788
SstI GAGCTC 1 cut(s) 168
TaaI ACNGT 1 cut(s) 27
TaiI ACGT 1 cut(s) 681
TaqI TCGA 7 cut(s) 78, 134, 390, 474, 777, 802, 827
TasI AATT 3 cut(s) 105, 236, 590
TfiI GAWTC 3 cut(s) 83, 695, 829
Tru1I TTAA 5 cut(s) 18, 29, 90, 99, 108
Tru9I TTAA 5 cut(s) 18, 29, 90, 99, 108
TseI GCWGC 1 cut(s) 285
TspDTI ATGAA 6 cut(s) 114, 229, 480, 522, 528, 662
Tth111I GACNNNGTC 1 cut(s) 410
XapI RAATTY 1 cut(s) 236
XbaI TCTAGA 2 cut(s) 50, 787
XceI RCATGY 1 cut(s) 326
XcmI CCANNNNNNNNNTGG 1 cut(s) 679
XmnI GAANNNNTTC 1 cut(s) 581
XspI CTAG 3 cut(s) 51, 455, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.