AT4G35985

response to cold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002216
Physical Location & Seq
Reverse (-)
17031945 .. 17034244
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35985.1

Sequence Viewer

Length: 1347 bp
ATGGAATGCTCTGCAACTCCTCCCAAGCTTTATCCAACCGTCGACACTTCAACCACCGTTGCTCCTCTCCCTAAGTCCTCTTCCTCCTCCTCTTCTACTAATAATAATAATCTCTATCCTTCAATCAATGTCAATGATCTTGTCAACAATATCTTCCCCGATCCCACCGCCTCTGACTCAGCTTCAGCGCCTCCTCTCGCGACGGAGGAAGTGATTCTCACAATCCACGGCGCGATGGTCCACCTCATTGACAAATCCTACAGCGTAGAGCTCGCTTGCGGCGATCTCGAGATTCTCCGTCTTGTTCAAGGAGATATCACCGTCGCGGTTTTCGCTCGCGTCGGTGACGAGATCCAATGGCCGTTGACTAAAGACGAACCCGCCGTTAAAGTCGATGAGTCTCATTACTTCTTCTCGCTCCGACCCGTTAAAGAATCCGAGTCATCGGATCATTCAGTCAACGAAACAGAGAACGAGATGTTGAACTACGGATTAACAATGGCTTCGAAAGGTCAAGAACCGATGCTTGAGAAGCTAGACAAGATCTTAGCTGATTACAGTAGTTTCACAGCGGAGGAGAAACAGAAAGAAGAGAACGTTTTGGATTTGACGGCGGCGAAGGAGACTTCACCGGAGGAGCTGAAGGGAAAGAGGAAAAAGATGGTGGAGAAGCAGTGTACGGCTTATTGGACGACTCTGGCTCCGAATGTTGAGGATTACAGTGGCGTTGCAGCGAAACTGATCGCTGCTGGTTCTGGTCAATTGATTAAAGGGATCTTATGGTGTGGGGATCTCACAATGGATAGACTCATGTGGGGAAATGATTTCATGAAGAAGAAGTTGTCCAAAGCAGAGAAGGAGAGACAAGTTAGTCCTGGAACTTTGAAACGTCTCAAAAGAGTAAAGAAGATGACAAAAATGACAGAGAAAGTGGCGAATGGTGTGCTCTCTGGTGTTGTTAAAGTTTCTGGATTCTTCTCGAGTTCAGTGATAAATAGCAAAGCTGGGCAGAAACTCTTTGGGCTTCTTCCTGGAGAAATGGTTCTTGCAACACTTGATGGATTCAACAAGGTTTGTGATGCTGTCGAAGTAGCAGGAAGACACGTTATGAAAACAACTTCAGATGTAACTACTGAAATCGTCGATCACAAGTATGGAGCAAAGACAGCACAAGCGACAAACGAAGGGCTTAGCGCAGCAGGGCATGCTTTTGGAACGGCGTGGACTGTTTTCAAGATTAGACAAGCTCTTAACCCCAAGAGTGCCATGAAGCCATCATCACTCGCTAAAACCGTAGTTAAAACGGCAGCTAAAGAGAGAAAGAAGGGGAAAAAAAGTTCTAAGTAG

Protein Analysis

448

Amino Acids

48.68

Weight (kDa)

8.66

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Senescence PF06911 247 - 434 5.9e-51 Senescence domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 870
AccI GTMKAC 1 cut(s) 42
AccII CGCG 4 cut(s) 200, 233, 326, 339
AciI CCGC 6 cut(s) 168, 279, 326, 381, 572, 614
AclI AACGTT 1 cut(s) 597
AclWI GGATC 5 cut(s) 155, 346, 456, 782, 798
AcoI YGGCCR 1 cut(s) 359
AcuI CTGAAG 3 cut(s) 168, 662, 1104
AfaI GTAC 1 cut(s) 679
AflIII ACRYGT 1 cut(s) 1102
AgsI TTSAA 7 cut(s) 51, 123, 308, 484, 886, 1066, 1234
AjnI CCWGG 2 cut(s) 874, 1030
AloI GAACNNNNNNTCC 3 cut(s) 1026, 1058, 1321
AluBI AGCT 9 cut(s) 28, 182, 271, 535, 551, 640, 1004, 1247, 1310
AluI AGCT 9 cut(s) 28, 182, 271, 535, 551, 640, 1004, 1247, 1310
Alw21I GWGCWC 2 cut(s) 273, 948
Alw26I GTCTC 4 cut(s) 405, 617, 856, 896
AlwI GGATC 5 cut(s) 155, 346, 456, 782, 798
Ama87I CYCGRG 2 cut(s) 287, 979
AoxI GGCC 1 cut(s) 359
ApeKI GCWGC 4 cut(s) 731, 746, 1196, 1307
Asp700I GAANNNNTTC 2 cut(s) 213, 1041
AspLEI GCGC 3 cut(s) 190, 233, 1196
AspS9I GGNCC 1 cut(s) 238
AsuHPI GGTGA 3 cut(s) 310, 356, 621
AsuII TTCGAA 1 cut(s) 506
AvaI CYCGRG 2 cut(s) 287, 979
AvaII GGWCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 1105
Bbv12I GWGCWC 2 cut(s) 273, 948
BbvI GCAGC 4 cut(s) 733, 743, 1208, 1319
BccI CCATC 4 cut(s) 229, 655, 1052, 1282
BceAI ACGGC 7 cut(s) 244, 346, 368, 627, 696, 1233, 1320
BcgI CGANNNNNNTGC 2 cut(s) 925, 959
BciT130I CCWGG 2 cut(s) 876, 1032
BcoDI GTCTC 4 cut(s) 405, 617, 856, 896
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 1 cut(s) 259
BfoI RGCGCY 1 cut(s) 191
BglII AGATCT 1 cut(s) 543
BisI GCNGC 6 cut(s) 280, 615, 732, 747, 1197, 1308
BlpI GCTNAGC 1 cut(s) 1190
BlsI GCNGC 6 cut(s) 281, 616, 733, 748, 1198, 1309
Bme1390I CCNGG 2 cut(s) 876, 1032
Bme18I GGWCC 1 cut(s) 238
BmeT110I CYCGRG 2 cut(s) 287, 979
BmgT120I GGNCC 1 cut(s) 238
BmiI GGNNCC 1 cut(s) 702
BmrFI CCNGG 2 cut(s) 876, 1032
BmsI GCATC 2 cut(s) 513, 1069
BpiI GAAGAC 1 cut(s) 1105
BpmI CTGGAG 1 cut(s) 1053
Bpu1102I GCTNAGC 1 cut(s) 1190
Bpu14I TTCGAA 1 cut(s) 506
BpuEI CTTGAG 1 cut(s) 548
BsaJI CCNNGG 1 cut(s) 226
BsaWI WCCGGW 1 cut(s) 631
BsaXI ACNNNNNCTCC 8 cut(s) 46, 76, 659, 685, 689, 715, 1026, 1056
BseBI CCWGG 2 cut(s) 876, 1032
BseDI CCNNGG 1 cut(s) 226
BseMII CTCAG 1 cut(s) 192
BseRI GAGGAG 7 cut(s) 9, 54, 76, 79, 183, 590, 650
BseXI GCAGC 4 cut(s) 733, 743, 1208, 1319
BseYI CCCAGC 1 cut(s) 1004
Bsh1236I CGCG 4 cut(s) 200, 233, 326, 339
BshFI GGCC 1 cut(s) 361
BsiHKAI GWGCWC 2 cut(s) 273, 948
BsiHKCI CYCGRG 2 cut(s) 287, 979
BsiSI CCGG 1 cut(s) 632
BsmAI GTCTC 4 cut(s) 405, 617, 856, 896
BsmBI CGTCTC 1 cut(s) 896
BsmI GAATGC 1 cut(s) 11
BsnI GGCC 1 cut(s) 361
BsoBI CYCGRG 2 cut(s) 287, 979
Bsp119I TTCGAA 1 cut(s) 506
Bsp1286I GDGCHC 2 cut(s) 273, 948
Bsp1720I GCTNAGC 1 cut(s) 1190
Bsp68I TCGCGA 1 cut(s) 200
BspACI CCGC 6 cut(s) 168, 279, 326, 381, 572, 614
BspANI GGCC 1 cut(s) 361
BspCNI CTCAG 1 cut(s) 191
BspFNI CGCG 4 cut(s) 200, 233, 326, 339
BspHI TCATGA 1 cut(s) 828
BspLI GGNNCC 1 cut(s) 702
BspPI GGATC 5 cut(s) 155, 346, 456, 782, 798
BspT104I TTCGAA 1 cut(s) 506
BssECI CCNNGG 1 cut(s) 226
Bst2UI CCWGG 2 cut(s) 876, 1032
Bst4CI ACNGT 7 cut(s) 40, 58, 322, 560, 722, 1228, 1294
Bst6I CTCTTC 3 cut(s) 85, 97, 585
BstAPI GCANNNNNTGC 1 cut(s) 1205
BstBI TTCGAA 1 cut(s) 506
BstC8I GCNNGC 4 cut(s) 273, 277, 337, 1206
BstDEI CTNAG 5 cut(s) 72, 178, 547, 1190, 1341
BstDSI CCRYGG 1 cut(s) 226
BstFNI CGCG 4 cut(s) 200, 233, 326, 339
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 3 cut(s) 190, 233, 1196
BstMAI GTCTC 4 cut(s) 405, 617, 856, 896
BstMWI GCNNNNNNNGC 4 cut(s) 332, 532, 1166, 1205
BstNI CCWGG 2 cut(s) 876, 1032
BstNSI RCATGY 1 cut(s) 1208
BstSCI CCNGG 2 cut(s) 874, 1030
BstSFI CTRYAG 1 cut(s) 259
BstUI CGCG 4 cut(s) 200, 233, 326, 339
BstV1I GCAGC 4 cut(s) 733, 743, 1208, 1319
BstV2I GAAGAC 1 cut(s) 1105
BstX2I RGATCY 4 cut(s) 351, 543, 774, 790
BstYI RGATCY 4 cut(s) 351, 543, 774, 790
BsuRI GGCC 1 cut(s) 361
BtgI CCRYGG 1 cut(s) 226
BtgZI GCGATG 1 cut(s) 248
BtsI GCAGTG 1 cut(s) 680
BtsIMutI CAGTG 3 cut(s) 680, 727, 993
BtuMI TCGCGA 1 cut(s) 200
Cac8I GCNNGC 4 cut(s) 273, 277, 337, 1206
CciI TCATGA 1 cut(s) 828
CfoI GCGC 3 cut(s) 190, 233, 1196
Cfr13I GGNCC 1 cut(s) 238
CseI GACGC 1 cut(s) 328
Csp6I GTAC 1 cut(s) 678
CviAII CATG 4 cut(s) 811, 829, 1205, 1267
CviQI GTAC 1 cut(s) 678
DdeI CTNAG 5 cut(s) 72, 178, 547, 1190, 1341
DrdI GACNNNNNNGTC 1 cut(s) 870
DseDI GACNNNNNNGTC 1 cut(s) 870
EaeI YGGCCR 1 cut(s) 359
Eam1104I CTCTTC 3 cut(s) 85, 97, 585
EarI CTCTTC 3 cut(s) 85, 97, 585
Ecl136II GAGCTC 1 cut(s) 271
Eco24I GRGCYC 1 cut(s) 273
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 271
Eco57I CTGAAG 3 cut(s) 168, 662, 1104
Eco88I CYCGRG 2 cut(s) 287, 979
EcoICRI GAGCTC 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 874, 1030
EcoRV GATATC 1 cut(s) 316
EcoT38I GRGCYC 1 cut(s) 273
Esp3I CGTCTC 1 cut(s) 896
FaeI CATG 4 cut(s) 814, 832, 1208, 1270
FaiI YATR 7 cut(s) 781, 812, 830, 1109, 1155, 1206, 1268
FatI CATG 4 cut(s) 810, 828, 1204, 1266
FauI CCCGC 1 cut(s) 388
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 6 cut(s) 280, 615, 732, 747, 1197, 1308
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 6 cut(s) 280, 615, 732, 747, 1197, 1308
FspBI CTAG 1 cut(s) 536
GlaI GCGC 3 cut(s) 189, 232, 1195
GluI GCNGC 6 cut(s) 280, 615, 732, 747, 1197, 1308
GsaI CCCAGC 1 cut(s) 1008
GsuI CTGGAG 1 cut(s) 1053
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 1 cut(s) 361
HapII CCGG 1 cut(s) 632
HgaI GACGC 1 cut(s) 328
HhaI GCGC 3 cut(s) 190, 233, 1196
Hin1II CATG 4 cut(s) 814, 832, 1208, 1270
Hin6I GCGC 3 cut(s) 188, 231, 1194
HinP1I GCGC 3 cut(s) 188, 231, 1194
HincII GTYRAC 4 cut(s) 43, 145, 366, 460
HindII GTYRAC 4 cut(s) 43, 145, 366, 460
HindIII AAGCTT 1 cut(s) 26
HpaII CCGG 1 cut(s) 632
HphI GGTGA 3 cut(s) 310, 356, 621
Hpy166II GTNNAC 7 cut(s) 43, 145, 241, 366, 460, 678, 1224
Hpy188I TCNGA 6 cut(s) 175, 422, 439, 448, 705, 1123
Hpy188III TCNNGA 8 cut(s) 199, 287, 289, 515, 829, 969, 979, 1234
Hpy8I GTNNAC 7 cut(s) 43, 145, 241, 366, 460, 678, 1224
Hpy99I CGWCG 5 cut(s) 44, 205, 326, 344, 1145
HpyAV CCTTC 6 cut(s) 129, 613, 637, 850, 1178, 1318
HpyCH4III ACNGT 7 cut(s) 40, 58, 322, 560, 722, 1228, 1294
HpyCH4IV ACGT 3 cut(s) 597, 889, 1104
HpyCH4V TGCA 3 cut(s) 14, 731, 1049
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 532, 1166, 1205
HpyF3I CTNAG 5 cut(s) 72, 178, 547, 1190, 1341
HpySE526I ACGT 3 cut(s) 597, 889, 1104
Hsp92II CATG 4 cut(s) 814, 832, 1208, 1270
HspAI GCGC 3 cut(s) 188, 231, 1194
LmnI GCTCC 5 cut(s) 67, 423, 637, 706, 1157
Lsp1109I GCAGC 4 cut(s) 733, 743, 1208, 1319
LweI GCATC 2 cut(s) 513, 1069
MaeI CTAG 1 cut(s) 536
MaeII ACGT 3 cut(s) 597, 889, 1104
MaeIII GTNAC 2 cut(s) 344, 1126
MfeI CAATTG 1 cut(s) 761
MflI RGATCY 4 cut(s) 351, 543, 774, 790
MhlI GDGCHC 2 cut(s) 273, 948
MluCI AATT 1 cut(s) 761
MlyI GAGTC 5 cut(s) 170, 407, 449, 688, 801
MmeI TCCRAC 2 cut(s) 59, 445
MroXI GAANNNNTTC 2 cut(s) 213, 1041
MseI TTAA 7 cut(s) 387, 429, 494, 768, 960, 1251, 1299
MslI CAYNNNNRTG 1 cut(s) 1152
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 1 cut(s) 632
MspR9I CCNGG 2 cut(s) 876, 1032
MunI CAATTG 1 cut(s) 761
Mva1269I GAATGC 1 cut(s) 11
MvaI CCWGG 2 cut(s) 876, 1032
MvnI CGCG 4 cut(s) 200, 233, 326, 339
MwoI GCNNNNNNNGC 4 cut(s) 332, 532, 1166, 1205
NlaIII CATG 4 cut(s) 814, 832, 1208, 1270
NlaIV GGNNCC 1 cut(s) 702
NmuCI GTSAC 1 cut(s) 344
NruI TCGCGA 1 cut(s) 200
NspI RCATGY 1 cut(s) 1208
NspV TTCGAA 1 cut(s) 506
PaeI GCATGC 1 cut(s) 1208
PaeR7I CTCGAG 2 cut(s) 287, 979
PagI TCATGA 1 cut(s) 828
PcsI WCGNNNNNNNCGW 3 cut(s) 279, 381, 1290
PctI GAATGC 1 cut(s) 11
PdmI GAANNNNTTC 2 cut(s) 213, 1041
PfeI GAWTC 5 cut(s) 214, 292, 434, 972, 1062
PfoI TCCNGGA 2 cut(s) 874, 1030
PkrI GCNGC 6 cut(s) 281, 616, 733, 748, 1198, 1309
PleI GAGTC 5 cut(s) 170, 406, 448, 688, 801
PpsI GAGTC 5 cut(s) 170, 406, 448, 688, 801
Psp124BI GAGCTC 1 cut(s) 273
Psp1406I AACGTT 1 cut(s) 597
Psp6I CCWGG 2 cut(s) 874, 1030
PspFI CCCAGC 1 cut(s) 1004
PspGI CCWGG 2 cut(s) 874, 1030
PspN4I GGNNCC 1 cut(s) 702
PspPI GGNCC 1 cut(s) 238
PsuI RGATCY 4 cut(s) 351, 543, 774, 790
RruI TCGCGA 1 cut(s) 200
RsaI GTAC 1 cut(s) 679
RsaNI GTAC 1 cut(s) 678
RseI CAYNNNNRTG 1 cut(s) 1152
SacI GAGCTC 1 cut(s) 273
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 7 cut(s) 387, 429, 494, 768, 960, 1251, 1299
SatI GCNGC 6 cut(s) 280, 615, 732, 747, 1197, 1308
Sau96I GGNCC 1 cut(s) 238
SchI GAGTC 5 cut(s) 170, 407, 449, 688, 801
ScrFI CCNGG 2 cut(s) 876, 1032
SduI GDGCHC 2 cut(s) 273, 948
SfaNI GCATC 2 cut(s) 513, 1069
SfcI CTRYAG 1 cut(s) 259
Sfr274I CTCGAG 2 cut(s) 287, 979
SfuI TTCGAA 1 cut(s) 506
SinI GGWCC 1 cut(s) 238
SlaI CTCGAG 2 cut(s) 287, 979
SmiMI CAYNNNNRTG 1 cut(s) 1152
SmlI CTYRAG 3 cut(s) 287, 527, 979
SmoI CTYRAG 3 cut(s) 287, 527, 979
SphI GCATGC 1 cut(s) 1208
Sse9I AATT 1 cut(s) 761
SsiI CCGC 6 cut(s) 168, 279, 326, 381, 572, 614
SspMI CTAG 1 cut(s) 536
SstI GAGCTC 1 cut(s) 273
StyD4I CCNGG 2 cut(s) 874, 1030
TaaI ACNGT 7 cut(s) 40, 58, 322, 560, 722, 1228, 1294
TaiI ACGT 3 cut(s) 600, 892, 1107
TaqI TCGA 7 cut(s) 42, 288, 393, 506, 980, 1086, 1143
TasI AATT 1 cut(s) 761
TauI GCSGC 2 cut(s) 282, 617
TfiI GAWTC 5 cut(s) 214, 292, 434, 972, 1062
Tru1I TTAA 7 cut(s) 387, 429, 494, 768, 960, 1251, 1299
Tru9I TTAA 7 cut(s) 387, 429, 494, 768, 960, 1251, 1299
TscAI CASTG 3 cut(s) 680, 727, 993
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 4 cut(s) 731, 746, 1196, 1307
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 4 cut(s) 817, 845, 1124, 1283
TspGWI ACGGA 3 cut(s) 218, 287, 504
TspRI CASTG 3 cut(s) 680, 727, 993
VpaK11BI GGWCC 1 cut(s) 238
XceI RCATGY 1 cut(s) 1208
XhoI CTCGAG 2 cut(s) 287, 979
XmiI GTMKAC 1 cut(s) 42
XmnI GAANNNNTTC 2 cut(s) 213, 1041
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.