Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002216

Physical Location & Seq

Genomic Coordinates Reverse (-)

10667200 .. 10670220

Gene Structure 3021 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016853

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1536 bp

ATGGAGTGCCCCAAACCTAAACCTAATCGCGTACGCTTTCAAGAACCAATGGAGTCTCCGGACCCTAATCGGAAGCCCAAACTGTACCCGGAGGTGGTCCAATCCAACCCGGACGCCCCCTCAATGTCGAACCCGAACCCGAAACCCGAACCGTCGGCCCCAAGTCTCTACCCCTCCCTCGACATGAAGGACCTCGTCGAGGATCTATTCCCGGAGAATCTATCATATTACGAAATCCCGCCAGAAAATCAATTCCCCGATAGCCCGACCTGCCGCGAACCTCCGTCGGCGCCGCCCGTCTCCGCCGAGGAGGTTCTTATTAAGATCCCCGGCTCGATCGTTAACCTAATTGATAAGAACTACAGCGTCGAGCTCGCTAGCGGCGACTTCTCGATCGTGCGGCTCGTCCAAGGCGACGACGTCGTTGCGATCCTCGCCCGTGTAGATGATGAGATCCAGTGGCCGCTGTTGAAGGACGAAGGCGTGGTCAAGCTCGACGATTCGCATTATTTTTTCTCGCTGTACGCGCCGGAGGATCATGAGTCGGCTCCGGCGCCGGAGCCGGAGTCCGGCAAGGATGGGAAGACGAAGGAGGAGAAGAAGAAGAAGAAGAAGAAGAAGGAGAAGGCGAAGAAGGATGAGAAGGAAACTAAGGAGGCTGATAATTTCTTGAACTACGGATTGACGATTGCGTCGAAAGGGCAGGAGAAATTGGTGAAGGAATTGGATGAGGTTTTGAAGAGTTACAGCAGTTTTTCGGTGCAGAAGGTTTCGGAGAAGGCGAAGAAGAAGGGGGAGGCATTGGATAGTTCCCTGGCATTGGAGACGGCGCCGGCAGAGTTGAGTTCGGAGAAGAAGAAGGAGATGAAGGAGCAGAGCGCTGCGTATTGGACCACATTGGCTCCCAATGTGGAGGACTATAATGGGAGTGCTGCTAAGTTGATTGCGGCCGGGACAGGGCAGCTGATCAGGGGGATTTTGTGGTGTGGAGATGTCACGGTGGAGAGGTTGAAATGGGGAAATGAGGTTATGAAGAAGAGGATGGAGCCGGGTTCAAATAAGGAAATCAGTCCCCAAACCATGAGGAGGATCAAAAGGGCCAAGAAAGTGAGCAAAACAACACAAAAAGTAGCAGCCGGTGTCCTCTCTGGGGTTTTGAAAGTTTCTGGATACTTCACAGGTGCGGTGGTGAATTCCAAAGTGGGAAAGAAATTCTTTCGTCTTCTGCCTGGGGAAATTTTGCTTGCTGGGCTGGATGGATTTTGTAAGGTCTGTGATGCTGTTGAAGTAGCCGGAAAAAATGTCATGTCAGCATCATCTACTGTTACAACTGAGCTTGTCTCTCACAAGTATGGTGAAGAAGCAGGTAAGGCGGCAGGTGAAGGGCTTGATGCAGCAGGACATGCCATAGGAACTGCATGGACTGTTTTCAAGATTCGAAAGGCAATGAACCCGAAAAGTGCTTTCAAATCCACCACCCTAGCCAAATCTGCTGCCAAGGCAAAAGCTGATGAGGTAAGGGCAAAGAAGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002239 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0007623 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009414 GO:0009415 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009628 GO:0009642 GO:0009644 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010035 GO:0016020 GO:0030054 GO:0033993 GO:0042221 GO:0043207 GO:0044464 GO:0048511 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071944 GO:0097305 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Senescence

Protein Analysis

512

Amino Acids

55.91

Weight (kDa)

8.82

Isoelectric Point (pI)

35.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Senescence	PF06911	314 - 500	2.5e-49	Senescence domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002216)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17840 AT2G17840 AT3G51250 AT3G51250 AT4G35985 AT4G35985 AT4G35985
fragaria_vesca	FvH4_1g11540 FvH4_1g11540 FvH4_1g11540 FvH4_1g11540 FvH4_2g01340 FvH4_2g01340 FvH4_2g01340
malus_domestica	MD02G1128400.v1.1 MD10G1123700.v1.1 MD15G1243600.v1.1
prunus_persica	Prupe.7G170600_v2.0.a1 Prupe.8G164100_v2.0.a1
pyrus_communis	pycom10g10590 pycom15g21450
rosa_chinensis	RchiOBHm_Chr2g0099181 RchiOBHm_Chr6g0252261
rosa_laevigata	RLG00000015400 RLG00000016853
rosa_multiflora	Rmu_co7982236.1_g000001 Rmu_sc0006252.1_g000017 Rmu_sc0006878.1_g000002
rosa_roxburghii	Rroxscaffold_2G00143380 Rroxscaffold_7G00216200
rosa_rugosa	Rorug02G0077500 Rorug05G0506500 Rorug05G0506600
rosa_samantha	Rh2AG126400 Rh2BG129600 Rh2CG130600 Rh2DG131000 Rh6AG020300 Rh6BG015900 Rh6CG013100 Rh6DG014400
rosa_wichuraiana	Rw2G009750 Rw6G001670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1367
AasI	GACNNNNNNGTC	1 cut(s)	691
AatII	GACGTC	1 cut(s)	423
Acc36I	ACCTGC	3 cut(s)	278, 1355, 1367
AccB1I	GGYRCC	3 cut(s)	289, 553, 829
AccII	CGCG	3 cut(s)	30, 276, 527
AccIII	TCCGGA	1 cut(s)	58
AclWI	GGATC	6 cut(s)	210, 319, 424, 448, 543, 1097
AcoI	YGGCCR	2 cut(s)	461, 948
AcsI	RAATTY	3 cut(s)	1192, 1211, 1236
AcyI	GRCGYC	5 cut(s)	114, 290, 420, 554, 830
AfaI	GTAC	3 cut(s)	33, 86, 524
AfeI	AGCGCT	1 cut(s)	880
AfiI	CCNNNNNNNGG	7 cut(s)	94, 199, 569, 820, 957, 1086, 1150
AjnI	CCWGG	2 cut(s)	813, 1228
AluBI	AGCT	5 cut(s)	373, 493, 964, 1336, 1508
AluI	AGCT	5 cut(s)	373, 493, 964, 1336, 1508
Alw21I	GWGCWC	1 cut(s)	375
Alw26I	GTCTC	5 cut(s)	60, 170, 304, 818, 1345
AlwI	GGATC	6 cut(s)	210, 319, 424, 448, 543, 1097
Aor13HI	TCCGGA	1 cut(s)	58
Aor51HI	AGCGCT	1 cut(s)	880
AoxI	GGCC	4 cut(s)	156, 461, 948, 1098
ApeKI	GCWGC	6 cut(s)	881, 932, 961, 1133, 1394, 1493
ApoI	RAATTY	3 cut(s)	1192, 1211, 1236
ArsI	GACNNNNNNTTYG	2 cut(s)	471, 503
AspLEI	GCGC	5 cut(s)	292, 529, 556, 832, 881
AspS9I	GGNCC	6 cut(s)	61, 97, 157, 190, 891, 1098
AsuC2I	CCSGG	6 cut(s)	89, 110, 212, 330, 952, 1050
AsuHPI	GGTGA	4 cut(s)	727, 1201, 1367, 1391
AsuII	TTCGAA	1 cut(s)	1438
AsuNHI	GCTAGC	1 cut(s)	377
AvaII	GGWCC	4 cut(s)	61, 97, 190, 891
BaeGI	GKGCMC	1 cut(s)	11
BanI	GGYRCC	3 cut(s)	289, 553, 829
BanII	GRGCYC	1 cut(s)	375
BbsI	GAAGAC	2 cut(s)	590, 1214
Bbv12I	GWGCWC	1 cut(s)	375
BbvI	GCAGC	6 cut(s)	868, 919, 973, 1145, 1406, 1480
BccI	CCATC	3 cut(s)	572, 1036, 1250
BceAI	ACGGC	1 cut(s)	843
BcgI	CGANNNNNNTGC	2 cut(s)	407, 441
BciT130I	CCWGG	2 cut(s)	815, 1230
BciVI	GTATCC	1 cut(s)	1163
BclI	TGATCA	1 cut(s)	966
BcnI	CCSGG	6 cut(s)	89, 110, 212, 330, 952, 1050
BcoDI	GTCTC	5 cut(s)	60, 170, 304, 818, 1345
BfaI	CTAG	2 cut(s)	378, 1481
BfmI	CTRYAG	1 cut(s)	361
BfoI	RGCGCY	4 cut(s)	293, 557, 833, 882
BfuAI	ACCTGC	3 cut(s)	278, 1355, 1367
BfuI	GTATCC	1 cut(s)	1163
Bme1390I	CCNGG	8 cut(s)	89, 110, 212, 330, 815, 952, 1050, 1230
Bme18I	GGWCC	4 cut(s)	61, 97, 190, 891
BmgT120I	GGNCC	6 cut(s)	61, 97, 157, 190, 891, 1098
BmiI	GGNNCC	9 cut(s)	63, 159, 291, 549, 555, 561, 831, 903, 1047
BmrFI	CCNGG	8 cut(s)	89, 110, 212, 330, 815, 952, 1050, 1230
BmsI	GCATC	3 cut(s)	1267, 1322, 1381
BmtI	GCTAGC	1 cut(s)	381
BpiI	GAAGAC	2 cut(s)	590, 1214
BplI	GAGNNNNNCTC	2 cut(s)	1325, 1357
Bpu14I	TTCGAA	1 cut(s)	1438
BpuMI	CCSGG	6 cut(s)	89, 110, 212, 330, 952, 1050
BsaHI	GRCGYC	5 cut(s)	114, 290, 420, 554, 830
BsaJI	CCNNGG	6 cut(s)	306, 328, 409, 813, 1229, 1497
BsaWI	WCCGGW	1 cut(s)	58
BsaXI	ACNNNNNCTCC	4 cut(s)	551, 581, 886, 916
Bsc4I	CCNNNNNNNGG	7 cut(s)	94, 199, 569, 820, 957, 1086, 1150
Bse118I	RCCGGY	2 cut(s)	832, 1136
Bse1I	ACTGG	1 cut(s)	457
Bse3DI	GCAATG	1 cut(s)	1452
BseAI	TCCGGA	1 cut(s)	58
BseBI	CCWGG	2 cut(s)	815, 1230
BseDI	CCNNGG	6 cut(s)	306, 328, 409, 813, 1229, 1497
BseGI	GGATG	5 cut(s)	583, 643, 733, 1047, 1261
BseLI	CCNNNNNNNGG	7 cut(s)	94, 199, 569, 820, 957, 1086, 1150
BseMI	GCAATG	1 cut(s)	1452
BseMII	CTCAG	1 cut(s)	1323
BseNI	ACTGG	1 cut(s)	457
BseRI	GAGGAG	3 cut(s)	323, 608, 1099
BseSI	GKGCMC	1 cut(s)	11
BseX3I	CGGCCG	1 cut(s)	948
BseXI	GCAGC	6 cut(s)	868, 919, 973, 1145, 1406, 1480
BseYI	CCCAGC	1 cut(s)	1247
BsgI	GTGCAG	1 cut(s)	782
Bsh1236I	CGCG	3 cut(s)	30, 276, 527
Bsh1285I	CGRYCG	3 cut(s)	339, 396, 951
BshFI	GGCC	4 cut(s)	158, 463, 950, 1100
BshNI	GGYRCC	3 cut(s)	289, 553, 829
BsiEI	CGRYCG	3 cut(s)	339, 396, 951
BsiHKAI	GWGCWC	1 cut(s)	375
BsiWI	CGTACG	1 cut(s)	31
BslFI	GGGAC	2 cut(s)	967, 1056
BslI	CCNNNNNNNGG	7 cut(s)	94, 199, 569, 820, 957, 1086, 1150
BsmAI	GTCTC	5 cut(s)	60, 170, 304, 818, 1345
BsmBI	CGTCTC	2 cut(s)	304, 818
BsmFI	GGGAC	2 cut(s)	967, 1056
BsnI	GGCC	4 cut(s)	158, 463, 950, 1100
Bsp119I	TTCGAA	1 cut(s)	1438
Bsp1286I	GDGCHC	2 cut(s)	11, 375
Bsp13I	TCCGGA	1 cut(s)	58
Bsp143I	GATC	9 cut(s)	202, 324, 336, 393, 429, 453, 535, 966, 1089
BspANI	GGCC	4 cut(s)	158, 463, 950, 1100
BspCNI	CTCAG	1 cut(s)	1324
BspEI	TCCGGA	1 cut(s)	58
BspFNI	CGCG	3 cut(s)	30, 276, 527
BspHI	TCATGA	1 cut(s)	538
BspLI	GGNNCC	9 cut(s)	63, 159, 291, 549, 555, 561, 831, 903, 1047
BspMI	ACCTGC	3 cut(s)	278, 1355, 1367
BspOI	GCTAGC	1 cut(s)	381
BspPI	GGATC	6 cut(s)	210, 319, 424, 448, 543, 1097
BspT104I	TTCGAA	1 cut(s)	1438
BspT107I	GGYRCC	3 cut(s)	289, 553, 829
BsrDI	GCAATG	1 cut(s)	1452
BsrFI	RCCGGY	2 cut(s)	832, 1136
BsrI	ACTGG	1 cut(s)	457
BssAI	RCCGGY	2 cut(s)	832, 1136
BssECI	CCNNGG	6 cut(s)	306, 328, 409, 813, 1229, 1497
BssMI	GATC	9 cut(s)	202, 324, 336, 393, 429, 453, 535, 966, 1089
BssNI	GRCGYC	5 cut(s)	114, 290, 420, 554, 830
BssT1I	CCWWGG	2 cut(s)	409, 1497
Bst2UI	CCWGG	2 cut(s)	815, 1230
Bst4CI	ACNGT	5 cut(s)	84, 153, 1000, 1324, 1426
Bst6I	CTCTTC	2 cut(s)	734, 1031
BstACI	GRCGYC	5 cut(s)	114, 290, 420, 554, 830
BstAPI	GCANNNNNTGC	1 cut(s)	1403
BstBI	TTCGAA	1 cut(s)	1438
BstC8I	GCNNGC	4 cut(s)	375, 379, 834, 1245
BstDEI	CTNAG	3 cut(s)	651, 936, 1332
BstENI	CCTNNNNNAGG	1 cut(s)	197
BstF5I	GGATG	5 cut(s)	583, 643, 733, 1047, 1261
BstFNI	CGCG	3 cut(s)	30, 276, 527
BstH2I	RGCGCY	4 cut(s)	293, 557, 833, 882
BstHHI	GCGC	5 cut(s)	292, 529, 556, 832, 881
BstKTI	GATC	9 cut(s)	205, 327, 339, 396, 432, 456, 538, 969, 1092
BstMAI	GTCTC	5 cut(s)	60, 170, 304, 818, 1345
BstMBI	GATC	9 cut(s)	202, 324, 336, 393, 429, 453, 535, 966, 1089
BstMCI	CGRYCG	3 cut(s)	339, 396, 951
BstMWI	GCNNNNNNNGC	8 cut(s)	270, 434, 526, 1249, 1370, 1403, 1490, 1499
BstNI	CCWGG	2 cut(s)	815, 1230
BstNSI	RCATGY	1 cut(s)	1406
BstSCI	CCNGG	8 cut(s)	87, 108, 210, 328, 813, 950, 1048, 1228
BstSFI	CTRYAG	1 cut(s)	361
BstSLI	GKGCMC	1 cut(s)	11
BstUI	CGCG	3 cut(s)	30, 276, 527
BstV1I	GCAGC	6 cut(s)	868, 919, 973, 1145, 1406, 1480
BstV2I	GAAGAC	2 cut(s)	590, 1214
BstX2I	RGATCY	3 cut(s)	202, 324, 453
BstYI	RGATCY	3 cut(s)	202, 324, 453
BstZI	CGGCCG	1 cut(s)	948
BsuI	GTATCC	1 cut(s)	1163
BsuRI	GGCC	4 cut(s)	158, 463, 950, 1100
BtsCI	GGATG	5 cut(s)	583, 643, 733, 1047, 1261
BtsIMutI	CAGTG	1 cut(s)	464
BveI	ACCTGC	3 cut(s)	278, 1355, 1367
Cac8I	GCNNGC	4 cut(s)	375, 379, 834, 1245
CciI	TCATGA	1 cut(s)	538
CfoI	GCGC	5 cut(s)	292, 529, 556, 832, 881
Cfr10I	RCCGGY	2 cut(s)	832, 1136
Cfr13I	GGNCC	6 cut(s)	61, 97, 157, 190, 891, 1098
CseI	GACGC	3 cut(s)	122, 355, 681
Csp6I	GTAC	3 cut(s)	32, 85, 523
CviAII	CATG	6 cut(s)	184, 539, 1081, 1306, 1403, 1419
CviQI	GTAC	3 cut(s)	32, 85, 523
DdeI	CTNAG	3 cut(s)	651, 936, 1332
DinI	GGCGCC	3 cut(s)	291, 555, 831
DpnI	GATC	9 cut(s)	204, 326, 338, 395, 431, 455, 537, 968, 1091
DpnII	GATC	9 cut(s)	202, 324, 336, 393, 429, 453, 535, 966, 1089
DrdI	GACNNNNNNGTC	1 cut(s)	691
DseDI	GACNNNNNNGTC	1 cut(s)	691
EaeI	YGGCCR	2 cut(s)	461, 948
EagI	CGGCCG	1 cut(s)	948
Eam1104I	CTCTTC	2 cut(s)	734, 1031
EarI	CTCTTC	2 cut(s)	734, 1031
EciI	GGCGGA	1 cut(s)	292
Ecl136II	GAGCTC	1 cut(s)	373
EclXI	CGGCCG	1 cut(s)	948
Eco130I	CCWWGG	2 cut(s)	409, 1497
Eco24I	GRGCYC	1 cut(s)	375
Eco47I	GGWCC	4 cut(s)	61, 97, 190, 891
Eco47III	AGCGCT	1 cut(s)	880
Eco52I	CGGCCG	1 cut(s)	948
Eco53kI	GAGCTC	1 cut(s)	373
EcoICRI	GAGCTC	1 cut(s)	373
EcoNI	CCTNNNNNAGG	1 cut(s)	197
EcoO109I	RGGNCCY	1 cut(s)	190
EcoRI	GAATTC	1 cut(s)	1192
EcoRII	CCWGG	2 cut(s)	813, 1228
EcoT14I	CCWWGG	2 cut(s)	409, 1497
EcoT38I	GRGCYC	1 cut(s)	375
EgeI	GGCGCC	3 cut(s)	291, 555, 831
EheI	GGCGCC	3 cut(s)	291, 555, 831
ErhI	CCWWGG	2 cut(s)	409, 1497
Esp3I	CGTCTC	2 cut(s)	304, 818
FaeI	CATG	6 cut(s)	187, 542, 1084, 1309, 1406, 1422
FalI	AAGNNNNNCTT	2 cut(s)	1199, 1231
FaqI	GGGAC	2 cut(s)	967, 1056
FatI	CATG	6 cut(s)	183, 538, 1080, 1305, 1402, 1418
FauI	CCCGC	1 cut(s)	246
FbaI	TGATCA	1 cut(s)	966
FokI	GGATG	5 cut(s)	590, 650, 740, 1054, 1268
FriOI	GRGCYC	1 cut(s)	375
FspBI	CTAG	2 cut(s)	378, 1481
GlaI	GCGC	5 cut(s)	291, 528, 555, 831, 880
GsaI	CCCAGC	1 cut(s)	1251
HaeII	RGCGCY	4 cut(s)	293, 557, 833, 882
HaeIII	GGCC	4 cut(s)	158, 463, 950, 1100
HgaI	GACGC	3 cut(s)	122, 355, 681
HhaI	GCGC	5 cut(s)	292, 529, 556, 832, 881
Hin1I	GRCGYC	5 cut(s)	114, 290, 420, 554, 830
Hin1II	CATG	6 cut(s)	187, 542, 1084, 1309, 1406, 1422
Hin6I	GCGC	5 cut(s)	290, 527, 554, 830, 879
HinP1I	GCGC	5 cut(s)	290, 527, 554, 830, 879
HincII	GTYRAC	1 cut(s)	343
HindII	GTYRAC	1 cut(s)	343
HinfI	GANTC	6 cut(s)	53, 217, 500, 542, 566, 1435
HpaI	GTTAAC	1 cut(s)	343
HphI	GGTGA	4 cut(s)	727, 1201, 1367, 1391
Hpy166II	GTNNAC	1 cut(s)	343
Hpy188I	TCNGA	3 cut(s)	72, 775, 850
Hpy188III	TCNNGA	7 cut(s)	41, 59, 391, 539, 670, 1167, 1432
Hpy8I	GTNNAC	1 cut(s)	343
Hpy99I	CGWCG	9 cut(s)	157, 200, 289, 371, 419, 422, 425, 500, 697
HpyCH4III	ACNGT	5 cut(s)	84, 153, 1000, 1324, 1426
HpyCH4IV	ACGT	1 cut(s)	420
HpyCH4V	TGCA	3 cut(s)	763, 1394, 1418
HpyF10VI	GCNNNNNNNGC	8 cut(s)	270, 434, 526, 1249, 1370, 1403, 1490, 1499
HpyF3I	CTNAG	3 cut(s)	651, 936, 1332
HpySE526I	ACGT	1 cut(s)	420
Hsp92I	GRCGYC	5 cut(s)	114, 290, 420, 554, 830
Hsp92II	CATG	6 cut(s)	187, 542, 1084, 1309, 1406, 1422
HspAI	GCGC	5 cut(s)	290, 527, 554, 830, 879
KasI	GGCGCC	3 cut(s)	289, 553, 829
Kpn2I	TCCGGA	1 cut(s)	58
KroI	GCCGGC	1 cut(s)	832
KroNI	GCCGGC	1 cut(s)	834
Ksp22I	TGATCA	1 cut(s)	966
KspAI	GTTAAC	1 cut(s)	343
Kzo9I	GATC	9 cut(s)	202, 324, 336, 393, 429, 453, 535, 966, 1089
LmnI	GCTCC	5 cut(s)	553, 559, 871, 907, 1045
Lsp1109I	GCAGC	6 cut(s)	868, 919, 973, 1145, 1406, 1480
LweI	GCATC	3 cut(s)	1267, 1322, 1381
MaeI	CTAG	2 cut(s)	378, 1481
MaeII	ACGT	1 cut(s)	420
MaeIII	GTNAC	3 cut(s)	743, 994, 1324
MalI	GATC	9 cut(s)	204, 326, 338, 395, 431, 455, 537, 968, 1091
MboI	GATC	9 cut(s)	202, 324, 336, 393, 429, 453, 535, 966, 1089
MflI	RGATCY	3 cut(s)	202, 324, 453
MhlI	GDGCHC	2 cut(s)	11, 375
MluCI	AATT	8 cut(s)	251, 348, 664, 710, 722, 1192, 1211, 1236
Mly113I	GGCGCC	3 cut(s)	290, 554, 830
MlyI	GAGTC	3 cut(s)	62, 551, 575
MmeI	TCCRAC	1 cut(s)	129
MroI	TCCGGA	1 cut(s)	58
MroNI	GCCGGC	1 cut(s)	832
MseI	TTAA	2 cut(s)	321, 342
MslI	CAYNNNNRTG	1 cut(s)	1350
MspA1I	CMGCKG	2 cut(s)	466, 964
MspR9I	CCNGG	8 cut(s)	89, 110, 212, 330, 815, 952, 1050, 1230
MvaI	CCWGG	2 cut(s)	815, 1230
MvnI	CGCG	3 cut(s)	30, 276, 527
MwoI	GCNNNNNNNGC	8 cut(s)	270, 434, 526, 1249, 1370, 1403, 1490, 1499
NaeI	GCCGGC	1 cut(s)	834
NarI	GGCGCC	3 cut(s)	290, 554, 830
NciI	CCSGG	6 cut(s)	89, 110, 212, 330, 952, 1050
NdeII	GATC	9 cut(s)	202, 324, 336, 393, 429, 453, 535, 966, 1089
NgoMIV	GCCGGC	1 cut(s)	832
NheI	GCTAGC	1 cut(s)	377
NlaIII	CATG	6 cut(s)	187, 542, 1084, 1309, 1406, 1422
NlaIV	GGNNCC	9 cut(s)	63, 159, 291, 549, 555, 561, 831, 903, 1047
NmeAIII	GCCGAG	1 cut(s)	331
NmuCI	GTSAC	1 cut(s)	994
NspI	RCATGY	1 cut(s)	1406
NspV	TTCGAA	1 cut(s)	1438
PagI	TCATGA	1 cut(s)	538
PaqCI	CACCTGC	1 cut(s)	1367
PcsI	WCGNNNNNNNCGW	5 cut(s)	381, 402, 411, 692, 779
PdiI	GCCGGC	1 cut(s)	834
PfeI	GAWTC	3 cut(s)	217, 500, 1435
Pfl23II	CGTACG	1 cut(s)	31
PflFI	GACNNNGTC	2 cut(s)	194, 419
PfoI	TCCNGGA	1 cut(s)	210
Ple19I	CGATCG	2 cut(s)	339, 396
PleI	GAGTC	3 cut(s)	61, 550, 574
PluTI	GGCGCC	3 cut(s)	293, 557, 833
PpsI	GAGTC	3 cut(s)	61, 550, 574
PpuMI	RGGWCCY	1 cut(s)	190
Psp124BI	GAGCTC	1 cut(s)	375
Psp5II	RGGWCCY	1 cut(s)	190
Psp6I	CCWGG	2 cut(s)	813, 1228
PspFI	CCCAGC	1 cut(s)	1247
PspGI	CCWGG	2 cut(s)	813, 1228
PspLI	CGTACG	1 cut(s)	31
PspN4I	GGNNCC	9 cut(s)	63, 159, 291, 549, 555, 561, 831, 903, 1047
PspPI	GGNCC	6 cut(s)	61, 97, 157, 190, 891, 1098
PspPPI	RGGWCCY	1 cut(s)	190
PsuI	RGATCY	3 cut(s)	202, 324, 453
PsyI	GACNNNGTC	2 cut(s)	194, 419
PvuI	CGATCG	2 cut(s)	339, 396
PvuII	CAGCTG	1 cut(s)	964
RsaI	GTAC	3 cut(s)	33, 86, 524
RsaNI	GTAC	3 cut(s)	32, 85, 523
RseI	CAYNNNNRTG	1 cut(s)	1350
SacI	GAGCTC	1 cut(s)	375
SaqAI	TTAA	2 cut(s)	321, 342
Sau3AI	GATC	9 cut(s)	202, 324, 336, 393, 429, 453, 535, 966, 1089
Sau96I	GGNCC	6 cut(s)	61, 97, 157, 190, 891, 1098
SchI	GAGTC	3 cut(s)	62, 551, 575
ScrFI	CCNGG	8 cut(s)	89, 110, 212, 330, 815, 952, 1050, 1230
SduI	GDGCHC	2 cut(s)	11, 375
SfaNI	GCATC	3 cut(s)	1267, 1322, 1381
SfcI	CTRYAG	1 cut(s)	361
SfoI	GGCGCC	3 cut(s)	291, 555, 831
SfuI	TTCGAA	1 cut(s)	1438
SinI	GGWCC	4 cut(s)	61, 97, 190, 891
SmiMI	CAYNNNNRTG	1 cut(s)	1350
Sse9I	AATT	8 cut(s)	251, 348, 664, 710, 722, 1192, 1211, 1236
SspDI	GGCGCC	3 cut(s)	289, 553, 829
SspMI	CTAG	2 cut(s)	378, 1481
SstI	GAGCTC	1 cut(s)	375
StyD4I	CCNGG	8 cut(s)	87, 108, 210, 328, 813, 950, 1048, 1228
StyI	CCWWGG	2 cut(s)	409, 1497
TaaI	ACNGT	5 cut(s)	84, 153, 1000, 1324, 1426
TaiI	ACGT	1 cut(s)	423
TaqI	TCGA	9 cut(s)	128, 180, 198, 335, 369, 392, 495, 695, 1438
TasI	AATT	8 cut(s)	251, 348, 664, 710, 722, 1192, 1211, 1236
TauI	GCSGC	7 cut(s)	276, 295, 384, 403, 466, 950, 1376
TfiI	GAWTC	3 cut(s)	217, 500, 1435
Tru1I	TTAA	2 cut(s)	321, 342
Tru9I	TTAA	2 cut(s)	321, 342
TscAI	CASTG	1 cut(s)	464
TseFI	GTSAC	1 cut(s)	994
TseI	GCWGC	6 cut(s)	881, 932, 961, 1133, 1394, 1493
Tsp45I	GTSAC	1 cut(s)	994
TspDTI	ATGAA	4 cut(s)	200, 881, 1046, 1463
TspGWI	ACGGA	2 cut(s)	273, 693
TspRI	CASTG	1 cut(s)	464
Tth111I	GACNNNGTC	2 cut(s)	194, 419
VpaK11BI	GGWCC	4 cut(s)	61, 97, 190, 891
XagI	CCTNNNNNAGG	1 cut(s)	197
XapI	RAATTY	3 cut(s)	1192, 1211, 1236
XceI	RCATGY	1 cut(s)	1406
XspI	CTAG	2 cut(s)	378, 1481
ZraI	GACGTC	1 cut(s)	421

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.