MD02G1128400.v1.1

Senescence-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002216
Physical Location & Seq
Reverse (-)
10392492 .. 10400176
7685 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1128400.v1.1.491

Sequence Viewer

Length: 621 bp
ATGGCGACGACATCGTCGCAGTCCTCGCCCGAGTCGGCGACGAGATCCAGTGACCTCTGGCGAAGAACGAAGGCGCTGTCAAGCTCGACGACTCGCATTACTTCTTCTCACTCTACGCTCCGCCGGATTCCGACTCCGGGTCCGAGTCGGACCCTAAATCGAAGGCGAACGATCCGGACAGTTTTTTTGAACTACGGATTGACGATCGCGTCAAAAGGGCAGGAGGGATTCGTGAAGGAATTGGATGGGATTTTGAAGAGTTACTGCAGTTTTTCAGTGCAGAAGGTTTCGGACATGGCGCGAAAGAAGGGGGAAGCTTTGGACGACACCCTTGCGTTGGAGACCTCGCCAGAGCAGTTGAGCTCGGAGAAAAAGGAGTTGGAGGAGCGGAGCGCCGCATATTGGACCACATTGGCACCGAATGTGGCGGATTACAATGGAATGGCTGCCAAATTGTTCGCACCCGGTTCAGGGCAGCTGATCAAGGGGATCTTGTGGTGTGGGGATGTGACGGTGGAGAGATTGAAATTGGGGAATGAGGTTAGGAAGAAGCGGATAGACCCGCGTTCGAATAAGGAAGTCAGTCCCCAAACCATGAGAAGGATTAAAATTAAGGGTTAA

Protein Analysis

207

Amino Acids

22.85

Weight (kDa)

10.12

Isoelectric Point (pI)

55.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Senescence PF06911 155 - 203 9.1e-08 Senescence domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 13, 208
AccB1I GGYRCC 1 cut(s) 415
AccBSI CCGCTC 1 cut(s) 388
AccII CGCG 3 cut(s) 209, 301, 565
AccIII TCCGGA 1 cut(s) 174
AciI CCGC 6 cut(s) 121, 388, 396, 428, 553, 563
AclWI GGATC 3 cut(s) 39, 166, 497
AfiI CCNNNNNNNGG 6 cut(s) 137, 337, 402, 470, 471, 600
AgsI TTSAA 3 cut(s) 190, 256, 526
AhdI GACNNNNNGTC 1 cut(s) 138
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AluBI AGCT 4 cut(s) 84, 317, 363, 478
AluI AGCT 4 cut(s) 84, 317, 363, 478
Alw21I GWGCWC 1 cut(s) 365
Alw26I GTCTC 1 cut(s) 335
AlwI GGATC 3 cut(s) 39, 166, 497
Ama87I CYCGRG 1 cut(s) 29
Aor13HI TCCGGA 1 cut(s) 174
ApeKI GCWGC 2 cut(s) 446, 475
ArsI GACNNNNNNTTYG 4 cut(s) 62, 94, 169, 201
AspLEI GCGC 3 cut(s) 76, 301, 395
AspS9I GGNCC 3 cut(s) 140, 150, 405
AsuC2I CCSGG 2 cut(s) 138, 465
AsuII TTCGAA 1 cut(s) 569
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 3 cut(s) 140, 150, 405
BanI GGYRCC 1 cut(s) 415
BanII GRGCYC 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 365
BbvI GCAGC 2 cut(s) 433, 487
BccI CCATC 1 cut(s) 239
BcgI CGANNNNNNTGC 2 cut(s) 314, 348
BclI TGATCA 1 cut(s) 480
BcnI CCSGG 2 cut(s) 138, 465
BcoDI GTCTC 1 cut(s) 335
BfmI CTRYAG 1 cut(s) 265
BfoI RGCGCY 2 cut(s) 77, 396
BisI GCNGC 3 cut(s) 396, 447, 476
BlsI GCNGC 3 cut(s) 397, 448, 477
Bme1390I CCNGG 2 cut(s) 138, 465
Bme18I GGWCC 3 cut(s) 140, 150, 405
BmeRI GACNNNNNGTC 1 cut(s) 138
BmeT110I CYCGRG 1 cut(s) 29
BmgT120I GGNCC 3 cut(s) 140, 150, 405
BmiI GGNNCC 3 cut(s) 141, 152, 417
BmrFI CCNGG 2 cut(s) 138, 465
Bpu14I TTCGAA 1 cut(s) 569
BpuMI CCSGG 2 cut(s) 138, 465
BsaI GGTCTC 1 cut(s) 335
BsaWI WCCGGW 1 cut(s) 174
Bsc4I CCNNNNNNNGG 6 cut(s) 137, 337, 402, 470, 471, 600
Bse1I ACTGG 1 cut(s) 48
BseAI TCCGGA 1 cut(s) 174
BseGI GGATG 2 cut(s) 250, 511
BseLI CCNNNNNNNGG 6 cut(s) 137, 337, 402, 470, 471, 600
BseNI ACTGG 1 cut(s) 48
BseRI GAGGAG 1 cut(s) 398
BseXI GCAGC 2 cut(s) 433, 487
BsgI GTGCAG 1 cut(s) 299
Bsh1236I CGCG 3 cut(s) 209, 301, 565
Bsh1285I CGRYCG 1 cut(s) 207
BshNI GGYRCC 1 cut(s) 415
BsiEI CGRYCG 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 365
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 4 cut(s) 124, 137, 175, 465
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 6 cut(s) 137, 337, 402, 470, 471, 600
BsmAI GTCTC 1 cut(s) 335
BsmFI GGGAC 1 cut(s) 570
Bso31I GGTCTC 1 cut(s) 335
BsoBI CYCGRG 1 cut(s) 29
Bsp119I TTCGAA 1 cut(s) 569
Bsp1286I GDGCHC 1 cut(s) 365
Bsp13I TCCGGA 1 cut(s) 174
Bsp143I GATC 5 cut(s) 44, 171, 204, 480, 489
BspACI CCGC 6 cut(s) 121, 388, 396, 428, 553, 563
BspEI TCCGGA 1 cut(s) 174
BspFNI CGCG 3 cut(s) 209, 301, 565
BspLI GGNNCC 3 cut(s) 141, 152, 417
BspMAI CTGCAG 1 cut(s) 269
BspPI GGATC 3 cut(s) 39, 166, 497
BspT104I TTCGAA 1 cut(s) 569
BspT107I GGYRCC 1 cut(s) 415
BspTNI GGTCTC 1 cut(s) 335
BsrBI CCGCTC 1 cut(s) 388
BsrI ACTGG 1 cut(s) 48
BssMI GATC 5 cut(s) 44, 171, 204, 480, 489
Bst4CI ACNGT 2 cut(s) 181, 514
Bst6I CTCTTC 1 cut(s) 251
BstBI TTCGAA 1 cut(s) 569
BstF5I GGATG 2 cut(s) 250, 511
BstFNI CGCG 3 cut(s) 209, 301, 565
BstH2I RGCGCY 2 cut(s) 77, 396
BstHHI GCGC 3 cut(s) 76, 301, 395
BstKTI GATC 5 cut(s) 47, 174, 207, 483, 492
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 5 cut(s) 44, 171, 204, 480, 489
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 1 cut(s) 25
BstSCI CCNGG 2 cut(s) 136, 463
BstSFI CTRYAG 1 cut(s) 265
BstUI CGCG 3 cut(s) 209, 301, 565
BstV1I GCAGC 2 cut(s) 433, 487
BstX2I RGATCY 2 cut(s) 44, 489
BstYI RGATCY 2 cut(s) 44, 489
BtsCI GGATG 2 cut(s) 250, 511
BtsIMutI CAGTG 2 cut(s) 55, 282
CfoI GCGC 3 cut(s) 76, 301, 395
Cfr13I GGNCC 3 cut(s) 140, 150, 405
CseI GACGC 1 cut(s) 198
CviAII CATG 2 cut(s) 295, 595
CviJI RGCY 5 cut(s) 84, 317, 363, 446, 478
CviKI_1 RGCY 5 cut(s) 84, 317, 363, 446, 478
DpnI GATC 5 cut(s) 46, 173, 206, 482, 491
DpnII GATC 5 cut(s) 44, 171, 204, 480, 489
DrdI GACNNNNNNGTC 2 cut(s) 13, 208
DriI GACNNNNNGTC 1 cut(s) 138
DseDI GACNNNNNNGTC 2 cut(s) 13, 208
Eam1104I CTCTTC 1 cut(s) 251
Eam1105I GACNNNNNGTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 2 cut(s) 110, 443
Ecl136II GAGCTC 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 365
Eco31I GGTCTC 1 cut(s) 335
Eco47I GGWCC 3 cut(s) 140, 150, 405
Eco53kI GAGCTC 1 cut(s) 363
Eco88I CYCGRG 1 cut(s) 29
EcoICRI GAGCTC 1 cut(s) 363
EcoT38I GRGCYC 1 cut(s) 365
FaeI CATG 2 cut(s) 298, 598
FaiI YATR 3 cut(s) 296, 400, 596
FalI AAGNNNNNCTT 2 cut(s) 476, 508
FaqI GGGAC 1 cut(s) 570
FatI CATG 2 cut(s) 294, 594
FauI CCCGC 1 cut(s) 570
FbaI TGATCA 1 cut(s) 480
Fnu4HI GCNGC 3 cut(s) 396, 447, 476
FokI GGATG 2 cut(s) 257, 518
FriOI GRGCYC 1 cut(s) 365
Fsp4HI GCNGC 3 cut(s) 396, 447, 476
GlaI GCGC 3 cut(s) 75, 300, 394
GluI GCNGC 3 cut(s) 396, 447, 476
HaeII RGCGCY 2 cut(s) 77, 396
HapII CCGG 4 cut(s) 124, 137, 175, 465
HgaI GACGC 1 cut(s) 198
HhaI GCGC 3 cut(s) 76, 301, 395
Hin1II CATG 2 cut(s) 298, 598
Hin6I GCGC 3 cut(s) 74, 299, 393
HinP1I GCGC 3 cut(s) 74, 299, 393
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 6 cut(s) 32, 91, 127, 133, 145, 228
HpaII CCGG 4 cut(s) 124, 137, 175, 465
Hpy188I TCNGA 5 cut(s) 132, 144, 150, 292, 367
Hpy188III TCNNGA 2 cut(s) 175, 232
Hpy99I CGWCG 4 cut(s) 10, 19, 43, 91
HpyAV CCTTC 6 cut(s) 64, 156, 229, 277, 301, 594
HpyCH4III ACNGT 2 cut(s) 181, 514
HpyCH4V TGCA 2 cut(s) 267, 280
HpyF10VI GCNNNNNNNGC 1 cut(s) 25
Hsp92II CATG 2 cut(s) 298, 598
HspAI GCGC 3 cut(s) 74, 299, 393
Kpn2I TCCGGA 1 cut(s) 174
Ksp22I TGATCA 1 cut(s) 480
Kzo9I GATC 5 cut(s) 44, 171, 204, 480, 489
LmnI GCTCC 3 cut(s) 123, 385, 390
LpnPI CCDG 9 cut(s) 43, 61, 137, 150, 188, 206, 363, 456, 478
Lsp1109I GCAGC 2 cut(s) 433, 487
MaeIII GTNAC 3 cut(s) 50, 260, 508
MalI GATC 5 cut(s) 46, 173, 206, 482, 491
MbiI CCGCTC 1 cut(s) 388
MboI GATC 5 cut(s) 44, 171, 204, 480, 489
MboII GAAGA 4 cut(s) 75, 96, 268, 559
MflI RGATCY 2 cut(s) 44, 489
MhlI GDGCHC 1 cut(s) 365
MluCI AATT 4 cut(s) 239, 452, 527, 609
MlyI GAGTC 4 cut(s) 41, 85, 127, 154
MmeI TCCRAC 4 cut(s) 128, 155, 318, 360
MnlI CCTC 6 cut(s) 34, 65, 217, 355, 376, 532
MroI TCCGGA 1 cut(s) 174
MseI TTAA 3 cut(s) 606, 612, 619
MspA1I CMGCKG 1 cut(s) 478
MspI CCGG 4 cut(s) 124, 137, 175, 465
MspR9I CCNGG 2 cut(s) 138, 465
MvnI CGCG 3 cut(s) 209, 301, 565
MwoI GCNNNNNNNGC 1 cut(s) 25
NciI CCSGG 2 cut(s) 138, 465
NdeII GATC 5 cut(s) 44, 171, 204, 480, 489
NlaIII CATG 2 cut(s) 298, 598
NlaIV GGNNCC 3 cut(s) 141, 152, 417
NmuCI GTSAC 2 cut(s) 50, 508
NspV TTCGAA 1 cut(s) 569
PfeI GAWTC 2 cut(s) 127, 228
PflFI GACNNNGTC 1 cut(s) 13
PkrI GCNGC 3 cut(s) 397, 448, 477
Ple19I CGATCG 1 cut(s) 207
PleI GAGTC 4 cut(s) 40, 85, 127, 153
PpsI GAGTC 4 cut(s) 40, 85, 127, 153
Psp124BI GAGCTC 1 cut(s) 365
PspN4I GGNNCC 3 cut(s) 141, 152, 417
PspPI GGNCC 3 cut(s) 140, 150, 405
PstI CTGCAG 1 cut(s) 269
PsuI RGATCY 2 cut(s) 44, 489
PsyI GACNNNGTC 1 cut(s) 13
PvuI CGATCG 1 cut(s) 207
PvuII CAGCTG 1 cut(s) 478
SacI GAGCTC 1 cut(s) 365
SaqAI TTAA 3 cut(s) 606, 612, 619
SatI GCNGC 3 cut(s) 396, 447, 476
Sau3AI GATC 5 cut(s) 44, 171, 204, 480, 489
Sau96I GGNCC 3 cut(s) 140, 150, 405
SchI GAGTC 4 cut(s) 41, 85, 127, 154
ScrFI CCNGG 2 cut(s) 138, 465
SduI GDGCHC 1 cut(s) 365
SetI ASST 8 cut(s) 57, 86, 288, 319, 347, 365, 480, 543
SfcI CTRYAG 1 cut(s) 265
SfuI TTCGAA 1 cut(s) 569
SinI GGWCC 3 cut(s) 140, 150, 405
Sse9I AATT 4 cut(s) 239, 452, 527, 609
SsiI CCGC 6 cut(s) 121, 388, 396, 428, 553, 563
SstI GAGCTC 1 cut(s) 365
StyD4I CCNGG 2 cut(s) 136, 463
TaaI ACNGT 2 cut(s) 181, 514
TaqI TCGA 3 cut(s) 86, 160, 569
TasI AATT 4 cut(s) 239, 452, 527, 609
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 2 cut(s) 127, 228
Tru1I TTAA 3 cut(s) 606, 612, 619
Tru9I TTAA 3 cut(s) 606, 612, 619
TscAI CASTG 2 cut(s) 55, 282
TseFI GTSAC 2 cut(s) 50, 508
TseI GCWGC 2 cut(s) 446, 475
Tsp45I GTSAC 2 cut(s) 50, 508
TspGWI ACGGA 1 cut(s) 210
TspRI CASTG 2 cut(s) 55, 282
Tth111I GACNNNGTC 1 cut(s) 13
VpaK11BI GGWCC 3 cut(s) 140, 150, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.