AT5G08380

Melibiase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003741
Physical Location & Seq
Reverse (-)
2694669 .. 2697781
3113 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G08380.2

Sequence Viewer

Length: 1233 bp
ATGTCGAGACGAGCAATGGTGATAAAGATGCCAATACTTATGATACTGATTTCATCAATGGTGATGACGATGGTCGAGTCTTCGAGATCAGTTAATAATGGTCATGACGATTCTGAGATCTTACGAAGACACTTGCTCACTAATGGCCTCGGAGTCACTCCTCCCATGGGGTGGAATAGCTGGAACCATTTTAGCTGCAACATAGATGAAAAAATGATCAAAGAAACTGCTGATGCTTTGGTTACCACTGGCCTCTCTAAGCTTGGCTACAACTATGTTAACATCGATGATTGCTGGGCTGAAATTTCTCGTGACAGCAAGGGAAGTCTGGTGCCAAAGAAGTCAACTTTCCCTTCTGGAATTAAAGCGGTTGCGGATTATGTTCACAGTAAAGGCCTTAAGCTTGGGATTTACTCAGATGCCGGGTATTTCACTTGCAGTAAAACCATGCCAGGTTCTTTAGGCTACGAGGAGCATGATGCCAAGACATTTGCAGAATGGGGAATTGATTACTTGAAGTATGACAACTGCAACAGCGATGGCTCTAAACCTACTGTCAGGTATCCAGTGATGACTCGAGCTCTGATGAAATCTGGCCGCCCCATCTTTCACTCATTGTGTGAATGGGGAGATATGCATCCAGCTCTATGGGGATCTCCAGTAGGCAACAGTTGGAGAACCACTAATGACATTAAAGATACTTGGCTTAGTATGATCTCAATAGCAGACATGAATGAAGTCTACGCTGAGCATGCAAGACCTGGTGGCTGGAACGACCCGGATATGCTTGAAGTGGGAAATGGAGGGATGACAAAAGACGAGTACATAGTCCATTTCAGCATATGGGCAATCTCAAAGGCTCCTCTTCTACTTGGTTGTGACATAAGAAACATGACCAAAGAAACAATGGAGATTGTCGCCAACAAGGAAGTCATTGCTATTAATCAAGATCCACATGGTGTTCAGGCCAAGAAAGTTAGAATGGAAGGCGATCTCGAGGTTTGGGCAGGACCATTGTCAGGTTACAGAGTAGCTTTGCTTCTATTGAACCGTGGACCATCGCGAACTTCGATTACTGCTCTTTGGGAGGATATTGAGATCCCTGCAAATAGCATCGTTGAAGCCAGAGATCTATGGGAGCACCAGACATTGAAGCAAAAGTTTGTGGGGAACTTGACAGCAACAGTGGATTCTCATGCGTGTAAGTTGTATGTTCTCAAACCTGTTGCCTGA

Protein Analysis

410

Amino Acids

45.71

Weight (kDa)

6.19

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Melibiase_2 PF16499 53 - 316 1.8e-78 Alpha galactosidase A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 331
AccB7I CCANNNNNTGG 1 cut(s) 171
AccI GTMKAC 1 cut(s) 741
AccII CGCG 1 cut(s) 1063
AciI CCGC 3 cut(s) 368, 374, 598
AclWI GGATC 3 cut(s) 661, 944, 1093
AcoI YGGCCR 1 cut(s) 595
AcsI RAATTY 1 cut(s) 303
AdeI CACNNNGTG 1 cut(s) 959
AfaI GTAC 1 cut(s) 824
AfiI CCNNNNNNNGG 3 cut(s) 167, 171, 1019
AflII CTTAAG 1 cut(s) 398
AgsI TTSAA 5 cut(s) 517, 791, 1048, 1121, 1153
AjnI CCWGG 2 cut(s) 451, 760
AluBI AGCT 7 cut(s) 180, 195, 262, 403, 581, 644, 1034
AluI AGCT 7 cut(s) 180, 195, 262, 403, 581, 644, 1034
Alw21I GWGCWC 2 cut(s) 583, 1143
AlwI GGATC 3 cut(s) 661, 944, 1093
Ama87I CYCGRG 2 cut(s) 576, 995
AoxI GGCC 5 cut(s) 145, 250, 394, 595, 966
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 1 cut(s) 303
AseI ATTAAT 1 cut(s) 942
AspS9I GGNCC 2 cut(s) 1010, 1055
AsuC2I CCSGG 2 cut(s) 424, 779
AsuHPI GGTGA 2 cut(s) 31, 73
AvaI CYCGRG 2 cut(s) 576, 995
AvaII GGWCC 2 cut(s) 1010, 1055
BanI GGYRCC 1 cut(s) 331
BanII GRGCYC 1 cut(s) 583
BarI GAAGNNNNNNTAC 2 cut(s) 1023, 1055
BauI CACGAG 1 cut(s) 309
BbsI GAAGAC 2 cut(s) 72, 133
Bbv12I GWGCWC 2 cut(s) 583, 1143
BbvI GCAGC 1 cut(s) 182
BccI CCATC 4 cut(s) 64, 533, 611, 1066
BciT130I CCWGG 2 cut(s) 453, 762
BciVI GTATCC 1 cut(s) 573
BclI TGATCA 1 cut(s) 216
BcnI CCSGG 2 cut(s) 424, 779
BfrI CTTAAG 1 cut(s) 398
BfuI GTATCC 1 cut(s) 573
BglII AGATCT 2 cut(s) 117, 1129
BisI GCNGC 2 cut(s) 196, 598
BlpI GCTNAGC 1 cut(s) 747
BlsI GCNGC 2 cut(s) 197, 599
Bme1390I CCNGG 4 cut(s) 424, 453, 762, 779
Bme18I GGWCC 2 cut(s) 1010, 1055
BmeT110I CYCGRG 2 cut(s) 576, 995
BmgT120I GGNCC 2 cut(s) 1010, 1055
BmiI GGNNCC 3 cut(s) 185, 333, 861
BmrFI CCNGG 4 cut(s) 424, 453, 762, 779
BmsI GCATC 6 cut(s) 18, 223, 409, 469, 646, 1122
BoxI GACNNNNGTC 2 cut(s) 71, 1015
BpiI GAAGAC 2 cut(s) 72, 133
BpmI CTGGAG 1 cut(s) 642
Bpu1102I GCTNAGC 1 cut(s) 747
BpuMI CCSGG 2 cut(s) 424, 779
Bsa29I ATCGAT 1 cut(s) 285
BsaBI GATNNNNATC 1 cut(s) 636
BsaJI CCNNGG 3 cut(s) 148, 165, 1051
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 171, 1019
Bse1I ACTGG 3 cut(s) 253, 566, 659
Bse3DI GCAATG 2 cut(s) 21, 933
Bse8I GATNNNNATC 1 cut(s) 636
BseBI CCWGG 2 cut(s) 453, 762
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 3 cut(s) 148, 165, 1051
BseGI GGATG 2 cut(s) 637, 813
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 3 cut(s) 167, 171, 1019
BseMI GCAATG 2 cut(s) 21, 933
BseMII CTCAG 3 cut(s) 105, 429, 738
BseNI ACTGG 3 cut(s) 253, 566, 659
BseRI GAGGAG 3 cut(s) 150, 485, 852
BseXI GCAGC 1 cut(s) 182
BseYI CCCAGC 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 1063
BshFI GGCC 5 cut(s) 147, 252, 396, 597, 968
BshNI GGYRCC 1 cut(s) 331
BshVI ATCGAT 1 cut(s) 285
BsiHKAI GWGCWC 2 cut(s) 583, 1143
BsiHKCI CYCGRG 2 cut(s) 576, 995
BsiSI CCGG 2 cut(s) 423, 779
BslI CCNNNNNNNGG 3 cut(s) 167, 171, 1019
BsnI GGCC 5 cut(s) 147, 252, 396, 597, 968
BsoBI CYCGRG 2 cut(s) 576, 995
Bsp1286I GDGCHC 2 cut(s) 583, 1143
Bsp143I GATC 9 cut(s) 86, 117, 216, 653, 714, 949, 991, 1098, 1129
Bsp1720I GCTNAGC 1 cut(s) 747
Bsp19I CCATGG 1 cut(s) 165
Bsp68I TCGCGA 1 cut(s) 1063
BspACI CCGC 3 cut(s) 368, 374, 598
BspANI GGCC 5 cut(s) 147, 252, 396, 597, 968
BspCNI CTCAG 3 cut(s) 106, 428, 739
BspDI ATCGAT 1 cut(s) 285
BspFNI CGCG 1 cut(s) 1063
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 3 cut(s) 185, 333, 861
BspPI GGATC 3 cut(s) 661, 944, 1093
BspT107I GGYRCC 1 cut(s) 331
BspTI CTTAAG 1 cut(s) 398
BsrDI GCAATG 2 cut(s) 21, 933
BsrI ACTGG 3 cut(s) 253, 566, 659
BssECI CCNNGG 3 cut(s) 148, 165, 1051
BssMI GATC 9 cut(s) 86, 117, 216, 653, 714, 949, 991, 1098, 1129
BssSI CACGAG 1 cut(s) 309
BssT1I CCWWGG 1 cut(s) 165
Bst2BI CACGAG 1 cut(s) 309
Bst2UI CCWGG 2 cut(s) 453, 762
Bst4CI ACNGT 5 cut(s) 389, 556, 671, 1052, 1186
Bst6I CTCTTC 1 cut(s) 870
BstAFI CTTAAG 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 753
BstDEI CTNAG 5 cut(s) 114, 258, 415, 707, 747
BstDSI CCRYGG 2 cut(s) 165, 1051
BstEII GGTNACC 1 cut(s) 241
BstF5I GGATG 2 cut(s) 637, 813
BstFNI CGCG 1 cut(s) 1063
BstKTI GATC 9 cut(s) 89, 120, 219, 656, 717, 952, 994, 1101, 1132
BstMBI GATC 9 cut(s) 86, 117, 216, 653, 714, 949, 991, 1098, 1129
BstMWI GCNNNNNNNGC 1 cut(s) 752
BstNI CCWGG 2 cut(s) 453, 762
BstNSI RCATGY 1 cut(s) 755
BstPAI GACNNNNGTC 2 cut(s) 71, 1015
BstPI GGTNACC 1 cut(s) 241
BstSCI CCNGG 4 cut(s) 422, 451, 760, 777
BstUI CGCG 1 cut(s) 1063
BstV1I GCAGC 1 cut(s) 182
BstV2I GAAGAC 2 cut(s) 72, 133
BstX2I RGATCY 5 cut(s) 117, 653, 949, 1098, 1129
BstXI CCANNNNNNTGG 1 cut(s) 648
BstYI RGATCY 5 cut(s) 117, 653, 949, 1098, 1129
Bsu15I ATCGAT 1 cut(s) 285
BsuI GTATCC 1 cut(s) 573
BsuRI GGCC 5 cut(s) 147, 252, 396, 597, 968
BsuTUI ATCGAT 1 cut(s) 285
BtgI CCRYGG 2 cut(s) 165, 1051
BtgZI GCGATG 2 cut(s) 552, 1044
BtsCI GGATG 2 cut(s) 637, 813
BtsIMutI CAGTG 3 cut(s) 246, 573, 1191
BtuMI TCGCGA 1 cut(s) 1063
Cac8I GCNNGC 1 cut(s) 753
CciI TCATGA 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 1010, 1055
ClaI ATCGAT 1 cut(s) 285
CsiI ACCWGGT 1 cut(s) 760
Csp6I GTAC 1 cut(s) 823
CviAII CATG 9 cut(s) 104, 166, 448, 476, 730, 752, 892, 956, 1196
CviQI GTAC 1 cut(s) 823
DdeI CTNAG 5 cut(s) 114, 258, 415, 707, 747
DpnI GATC 9 cut(s) 88, 119, 218, 655, 716, 951, 993, 1100, 1131
DpnII GATC 9 cut(s) 86, 117, 216, 653, 714, 949, 991, 1098, 1129
DraIII CACNNNGTG 1 cut(s) 959
EaeI YGGCCR 1 cut(s) 595
Eam1104I CTCTTC 1 cut(s) 870
EarI CTCTTC 1 cut(s) 870
Ecl136II GAGCTC 1 cut(s) 581
Eco130I CCWWGG 1 cut(s) 165
Eco147I AGGCCT 1 cut(s) 396
Eco24I GRGCYC 1 cut(s) 583
Eco47I GGWCC 2 cut(s) 1010, 1055
Eco53kI GAGCTC 1 cut(s) 581
Eco88I CYCGRG 2 cut(s) 576, 995
Eco91I GGTNACC 1 cut(s) 241
EcoICRI GAGCTC 1 cut(s) 581
EcoO65I GGTNACC 1 cut(s) 241
EcoRII CCWGG 2 cut(s) 451, 760
EcoT14I CCWWGG 1 cut(s) 165
EcoT22I ATGCAT 1 cut(s) 639
EcoT38I GRGCYC 1 cut(s) 583
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 9 cut(s) 107, 169, 451, 479, 733, 755, 895, 959, 1199
FatI CATG 9 cut(s) 103, 165, 447, 475, 729, 751, 891, 955, 1195
FauNDI CATATG 1 cut(s) 842
FbaI TGATCA 1 cut(s) 216
FblI GTMKAC 1 cut(s) 741
Fnu4HI GCNGC 2 cut(s) 196, 598
FokI GGATG 2 cut(s) 624, 820
FriOI GRGCYC 1 cut(s) 583
Fsp4HI GCNGC 2 cut(s) 196, 598
GluI GCNGC 2 cut(s) 196, 598
GsaI CCCAGC 1 cut(s) 298
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 5 cut(s) 147, 252, 396, 597, 968
HapII CCGG 2 cut(s) 423, 779
Hin1II CATG 9 cut(s) 107, 169, 451, 479, 733, 755, 895, 959, 1199
HincII GTYRAC 2 cut(s) 280, 345
HindII GTYRAC 2 cut(s) 280, 345
HindIII AAGCTT 2 cut(s) 260, 401
HinfI GANTC 5 cut(s) 77, 110, 153, 574, 1190
HpaI GTTAAC 1 cut(s) 280
HpaII CCGG 2 cut(s) 423, 779
HphI GGTGA 2 cut(s) 31, 73
Hpy166II GTNNAC 5 cut(s) 280, 345, 385, 742, 1055
Hpy188I TCNGA 4 cut(s) 115, 152, 418, 585
Hpy188III TCNNGA 8 cut(s) 6, 84, 104, 311, 357, 947, 995, 1062
Hpy8I GTNNAC 5 cut(s) 280, 345, 385, 742, 1055
HpyAV CCTTC 2 cut(s) 363, 980
HpyCH4III ACNGT 5 cut(s) 389, 556, 671, 1052, 1186
HpyCH4V TGCA 7 cut(s) 198, 438, 494, 531, 637, 755, 1106
HpyF10VI GCNNNNNNNGC 1 cut(s) 752
HpyF3I CTNAG 5 cut(s) 114, 258, 415, 707, 747
Hsp92II CATG 9 cut(s) 107, 169, 451, 479, 733, 755, 895, 959, 1199
Ksp22I TGATCA 1 cut(s) 216
KspAI GTTAAC 1 cut(s) 280
Kzo9I GATC 9 cut(s) 86, 117, 216, 653, 714, 949, 991, 1098, 1129
LmnI GCTCC 3 cut(s) 472, 865, 1138
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 6 cut(s) 18, 223, 409, 469, 646, 1122
MabI ACCWGGT 1 cut(s) 760
MaeIII GTNAC 5 cut(s) 154, 241, 311, 878, 1022
MalI GATC 9 cut(s) 88, 119, 218, 655, 716, 951, 993, 1100, 1131
MboI GATC 9 cut(s) 86, 117, 216, 653, 714, 949, 991, 1098, 1129
MboII GAAGA 3 cut(s) 72, 138, 857
MflI RGATCY 5 cut(s) 117, 653, 949, 1098, 1129
MhlI GDGCHC 2 cut(s) 583, 1143
MluCI AATT 3 cut(s) 303, 360, 504
MlyI GAGTC 3 cut(s) 86, 162, 568
MmeI TCCRAC 1 cut(s) 653
MnlI CCTC 8 cut(s) 158, 171, 263, 463, 797, 873, 991, 1081
Mph1103I ATGCAT 1 cut(s) 639
MseI TTAA 6 cut(s) 93, 279, 363, 399, 693, 942
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 2 cut(s) 423, 779
MspR9I CCNGG 4 cut(s) 424, 453, 762, 779
MvaI CCWGG 2 cut(s) 453, 762
MvnI CGCG 1 cut(s) 1063
MwoI GCNNNNNNNGC 1 cut(s) 752
NciI CCSGG 2 cut(s) 424, 779
NcoI CCATGG 1 cut(s) 165
NdeI CATATG 1 cut(s) 842
NdeII GATC 9 cut(s) 86, 117, 216, 653, 714, 949, 991, 1098, 1129
NlaIII CATG 9 cut(s) 107, 169, 451, 479, 733, 755, 895, 959, 1199
NlaIV GGNNCC 3 cut(s) 185, 333, 861
NmuCI GTSAC 3 cut(s) 154, 311, 878
NruI TCGCGA 1 cut(s) 1063
NsiI ATGCAT 1 cut(s) 639
NspI RCATGY 1 cut(s) 755
PaeI GCATGC 1 cut(s) 755
PaeR7I CTCGAG 2 cut(s) 576, 995
PagI TCATGA 1 cut(s) 103
PceI AGGCCT 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 1067
PfeI GAWTC 2 cut(s) 110, 1190
PflMI CCANNNNNTGG 1 cut(s) 171
PkrI GCNGC 2 cut(s) 197, 599
PleI GAGTC 3 cut(s) 85, 161, 568
PpsI GAGTC 3 cut(s) 85, 161, 568
PshAI GACNNNNGTC 2 cut(s) 71, 1015
PshBI ATTAAT 1 cut(s) 942
Psp124BI GAGCTC 1 cut(s) 583
Psp6I CCWGG 2 cut(s) 451, 760
PspEI GGTNACC 1 cut(s) 241
PspFI CCCAGC 1 cut(s) 294
PspGI CCWGG 2 cut(s) 451, 760
PspN4I GGNNCC 3 cut(s) 185, 333, 861
PspPI GGNCC 2 cut(s) 1010, 1055
PspXI VCTCGAGB 1 cut(s) 576
PsrI GAACNNNNNNTAC 2 cut(s) 1057, 1089
PsuI RGATCY 5 cut(s) 117, 653, 949, 1098, 1129
RruI TCGCGA 1 cut(s) 1063
RsaI GTAC 1 cut(s) 824
RsaNI GTAC 1 cut(s) 823
SacI GAGCTC 1 cut(s) 583
SaqAI TTAA 6 cut(s) 93, 279, 363, 399, 693, 942
SatI GCNGC 2 cut(s) 196, 598
Sau3AI GATC 9 cut(s) 86, 117, 216, 653, 714, 949, 991, 1098, 1129
Sau96I GGNCC 2 cut(s) 1010, 1055
SchI GAGTC 3 cut(s) 86, 162, 568
ScrFI CCNGG 4 cut(s) 424, 453, 762, 779
SduI GDGCHC 2 cut(s) 583, 1143
SexAI ACCWGGT 1 cut(s) 760
SfaNI GCATC 6 cut(s) 18, 223, 409, 469, 646, 1122
Sfr274I CTCGAG 2 cut(s) 576, 995
SinI GGWCC 2 cut(s) 1010, 1055
SlaI CTCGAG 2 cut(s) 576, 995
SmlI CTYRAG 3 cut(s) 398, 576, 995
SmoI CTYRAG 3 cut(s) 398, 576, 995
SphI GCATGC 1 cut(s) 755
Sse9I AATT 3 cut(s) 303, 360, 504
SseBI AGGCCT 1 cut(s) 396
SsiI CCGC 3 cut(s) 368, 374, 598
SstI GAGCTC 1 cut(s) 583
StuI AGGCCT 1 cut(s) 396
StyD4I CCNGG 4 cut(s) 422, 451, 760, 777
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 5 cut(s) 389, 556, 671, 1052, 1186
TaqI TCGA 7 cut(s) 5, 75, 83, 285, 577, 996, 1070
TasI AATT 3 cut(s) 303, 360, 504
TatI WGTACW 1 cut(s) 822
TauI GCSGC 1 cut(s) 600
TfiI GAWTC 2 cut(s) 110, 1190
Tru1I TTAA 6 cut(s) 93, 279, 363, 399, 693, 942
Tru9I TTAA 6 cut(s) 93, 279, 363, 399, 693, 942
TscAI CASTG 3 cut(s) 253, 573, 1191
TseFI GTSAC 3 cut(s) 154, 311, 878
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 3 cut(s) 154, 311, 878
TspDTI ATGAA 5 cut(s) 42, 222, 602, 746, 750
TspRI CASTG 3 cut(s) 253, 573, 1191
Van91I CCANNNNNTGG 1 cut(s) 171
Vha464I CTTAAG 1 cut(s) 398
VpaK11BI GGWCC 2 cut(s) 1010, 1055
VspI ATTAAT 1 cut(s) 942
XapI RAATTY 1 cut(s) 303
XceI RCATGY 1 cut(s) 755
XcmI CCANNNNNNNNNTGG 1 cut(s) 904
XhoI CTCGAG 2 cut(s) 576, 995
XmiI GTMKAC 1 cut(s) 741
Zsp2I ATGCAT 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.