Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003741

Physical Location & Seq

Genomic Coordinates Forward (+)

64184567 .. 64187814

Gene Structure 3248 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G037690.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1278 bp

ATGGTGAATATGAGGGGGAAAAGTGGCGGCGATGCTGCAGTAACAACGAGGATGATTGTAATGACGGTGATGATGGTATGCTCATTCATATCACCACAACCGATGTTAATGGGTGTTTACGCTTCACCGATAACAAGAATGATGGTGGCAGATACCAAAGAGCAACAGAGACGAAATCTGCTTGCTAATGGGCTTGGAGTGACTCCTCCCATGGGGTGGAATAGTTGGAATCACTTCAACTGCAAAATTGATGAGAAAATCATCAAAGCAACTGCTGATGCTCTGATTTCCACTGGTCTCTCTAAACTTGGATATACCTATGTTAACATAGATGATTGTTGGGCAGAAGTAGCTCGTGATGACAAGGGTAATCTAGTGCCCAAGAAATCCACATTTCCATCTGGCATCAAAGCTCTTGCGGATTATGTGCACAGCAAGGGATTAAAGCTAGGAATCTACTCAGATGCAGGGTACTTTACTTGCAGCAAAACCATGCCTGGTTCACTTGGTCACGAGGAACAAGATGCCAAAATTTTTGCTGCATGGGGTATTGATTATTTGAAGTATGACAACTGTAATACTGATGGCTCCAAACCAACTGTTAGATATCCTGTCATGACCCGAGCACTGATGAAAGCAGACCGTCCCATATTCTTTTCTCTGTGTGAATGGGGAGATTTGCATCCTGCTTTATGGGGTGCTAAAGTCGGAAACAGCTGGAGAACTACTAATGACATTTCTGATACATGGGAAAGTATGGTCTCCAGAGCAGACATGAATGAAGTTTATGCTGAATATGCCAGACCTGGTGGTTGGAATGATCCTGACATGCTTGAGGTGGGAAATGGAGGAATGACAAAGGATGAATATGTAGTCCATTTTAGCATATGGGCCATATCTAAGGCTCCCCTTCTTATTGGTTGTGATGTGAGGAATATGACAAAAGAGACTATGGAGATCATCGCAAATAAAGAGGTTATCTCTGTCAACCAAGATCCACTTGGTGTCCAAGCTAAAAAGGTTAGAATGGAAGGGGATCTTGAGGTTTGGGCTGGGCCCCTTTCAGGCTACAGAGTTGCCTTACTCCTTGTCAACCGAGGCCCGTGGCGAACATCCATTACAGCCCACTGGGAAGACATTGGTATCCCCCCAAACAGTGTAGTTGAAGCAAGAGATCTTTGGGAGCACAAGACACTGAAGAAACGATTTGTTGGAAACTTGACAGCCACCATGGACTCCCATGGATGCAAACTGTATGTGCTGAAGCCAATCTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0048046 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603

Pfam Domains

Protein Families

Melibiase_2 Melibiase_2_C

Protein Analysis

425

Amino Acids

47.25

Weight (kDa)

7.56

Isoelectric Point (pI)

18.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	68 - 332	2.4e-74	Alpha galactosidase A
Melibiase_C	PF17801	344 - 421	3.6e-18	Alpha galactosidase C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003741)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08380 AT5G08380
fragaria_vesca	FvH4_2g12370 FvH4_2g12370 FvH4_2g12370 FvH4_4g37361
malus_domestica	MD13G1000400.v1.1 MD16G1012600.v1.1
prunus_persica	Prupe.1G352200_v2.0.a1 Prupe.1G352200_v2.0.a1
pyrus_communis	pycom13g00040 pycom16g01180 pycom2749g00030
rosa_chinensis	RchiOBHm_Chr4g0447241 RchiOBHm_Chr6g0272391
rosa_laevigata	RLG00000005594 RLG00000013676
rosa_multiflora	Rmu_co8492007.1_g000001 Rmu_sc0018949.1_g000001 Rmu_sc0018950.1_g000001
rosa_roxburghii	Rroxscaffold_5G00387600 Rroxscaffold_7G00196300
rosa_rugosa	Rorug04G0379000 Rorug06G0069000
rosa_samantha	Rh4AG440500 Rh4BG449500 Rh4CG466600 Rh4DG448000 Rh6AG184900 Rh6BG188200 Rh6CG186100 Rh6DG177900
rosa_wichuraiana	Rw4G037690 Rw6G015990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	216
AciI	CCGC	2 cut(s)	27, 419
AclWI	GGATC	3 cut(s)	815, 989, 1044
AcsI	RAATTY	1 cut(s)	531
AcuI	CTGAAG	1 cut(s)	1217
AdeI	CACNNNGTG	1 cut(s)	1004
AfaI	GTAC	1 cut(s)	473
AfiI	CCNNNNNNNGG	3 cut(s)	212, 216, 1064
AgsI	TTSAA	3 cut(s)	238, 562, 1166
AjnI	CCWGG	2 cut(s)	496, 805
AluBI	AGCT	5 cut(s)	353, 413, 448, 717, 1013
AluI	AGCT	5 cut(s)	353, 413, 448, 717, 1013
Alw21I	GWGCWC	3 cut(s)	432, 628, 1188
Alw26I	GTCTC	4 cut(s)	163, 302, 766, 941
Alw44I	GTGCAC	1 cut(s)	428
AlwI	GGATC	3 cut(s)	815, 989, 1044
Ama87I	CYCGRG	1 cut(s)	621
AoxI	GGCC	3 cut(s)	891, 1055, 1099
ApaI	GGGCCC	1 cut(s)	1059
ApaLI	GTGCAC	1 cut(s)	428
ApeKI	GCWGC	3 cut(s)	35, 483, 539
ApoI	RAATTY	1 cut(s)	531
Asp700I	GAANNNNTTC	1 cut(s)	233
AspS9I	GGNCC	4 cut(s)	891, 1055, 1056, 1100
AsuHPI	GGTGA	4 cut(s)	16, 79, 84, 117
AvaI	CYCGRG	1 cut(s)	621
BaeGI	GKGCMC	3 cut(s)	381, 432, 1059
BaeI	ACNNNNGTAYC	2 cut(s)	1126, 1159
BanII	GRGCYC	1 cut(s)	1059
BauI	CACGAG	2 cut(s)	354, 512
BbsI	GAAGAC	1 cut(s)	1140
Bbv12I	GWGCWC	3 cut(s)	432, 628, 1188
BbvI	GCAGC	3 cut(s)	22, 495, 526
BccI	CCATC	4 cut(s)	67, 136, 406, 578
BciT130I	CCWGG	2 cut(s)	498, 807
BciVI	GTATCC	1 cut(s)	1154
BcoDI	GTCTC	4 cut(s)	163, 302, 766, 941
BfaI	CTAG	2 cut(s)	374, 449
BfmI	CTRYAG	2 cut(s)	36, 1069
BfuI	GTATCC	1 cut(s)	1154
BglII	AGATCT	1 cut(s)	1174
BisI	GCNGC	4 cut(s)	28, 36, 484, 540
BlsI	GCNGC	4 cut(s)	29, 37, 485, 541
Bme1390I	CCNGG	2 cut(s)	498, 807
BmeT110I	CYCGRG	1 cut(s)	621
BmgT120I	GGNCC	4 cut(s)	891, 1055, 1056, 1100
BmiI	GGNNCC	4 cut(s)	589, 906, 1057, 1058
BmrFI	CCNGG	2 cut(s)	498, 807
BmrI	ACTGGG	1 cut(s)	1138
BmsI	GCATC	7 cut(s)	22, 268, 414, 454, 514, 691, 1235
BmuI	ACTGGG	1 cut(s)	1138
BpiI	GAAGAC	1 cut(s)	1140
BplI	GAGNNNNNCTC	2 cut(s)	965, 997
BpmI	CTGGAG	2 cut(s)	739, 748
BpuEI	CTTGAG	2 cut(s)	854, 1061
BsaBI	GATNNNNATC	1 cut(s)	681
BsaI	GGTCTC	2 cut(s)	302, 766
BsaJI	CCNNGG	5 cut(s)	210, 1096, 1103, 1230, 1240
Bsc4I	CCNNNNNNNGG	3 cut(s)	212, 216, 1064
Bse1I	ACTGG	2 cut(s)	298, 1133
Bse8I	GATNNNNATC	1 cut(s)	681
BseBI	CCWGG	2 cut(s)	498, 807
BseDI	CCNNGG	5 cut(s)	210, 1096, 1103, 1230, 1240
BseGI	GGATG	5 cut(s)	57, 682, 868, 1112, 1250
BseJI	GATNNNNATC	1 cut(s)	681
BseLI	CCNNNNNNNGG	3 cut(s)	212, 216, 1064
BseMII	CTCAG	1 cut(s)	474
BseNI	ACTGG	2 cut(s)	298, 1133
BseRI	GAGGAG	1 cut(s)	195
BseSI	GKGCMC	3 cut(s)	381, 432, 1059
BseXI	GCAGC	3 cut(s)	22, 495, 526
BseYI	CCCAGC	1 cut(s)	1052
BshFI	GGCC	3 cut(s)	893, 1057, 1101
BsiHKAI	GWGCWC	3 cut(s)	432, 628, 1188
BsiHKCI	CYCGRG	1 cut(s)	621
BslFI	GGGAC	1 cut(s)	630
BslI	CCNNNNNNNGG	3 cut(s)	212, 216, 1064
BsmAI	GTCTC	4 cut(s)	163, 302, 766, 941
BsmBI	CGTCTC	1 cut(s)	163
BsmFI	GGGAC	1 cut(s)	630
BsnI	GGCC	3 cut(s)	893, 1057, 1101
Bso31I	GGTCTC	2 cut(s)	302, 766
BsoBI	CYCGRG	1 cut(s)	621
Bsp120I	GGGCCC	1 cut(s)	1055
Bsp1286I	GDGCHC	5 cut(s)	381, 432, 628, 1059, 1188
Bsp143I	GATC	5 cut(s)	820, 957, 994, 1036, 1174
Bsp19I	CCATGG	3 cut(s)	210, 1230, 1240
BspACI	CCGC	2 cut(s)	27, 419
BspANI	GGCC	3 cut(s)	893, 1057, 1101
BspCNI	CTCAG	1 cut(s)	473
BspHI	TCATGA	1 cut(s)	615
BspLI	GGNNCC	4 cut(s)	589, 906, 1057, 1058
BspMAI	CTGCAG	1 cut(s)	40
BspPI	GGATC	3 cut(s)	815, 989, 1044
BspTNI	GGTCTC	2 cut(s)	302, 766
BsrI	ACTGG	2 cut(s)	298, 1133
BssECI	CCNNGG	5 cut(s)	210, 1096, 1103, 1230, 1240
BssMI	GATC	5 cut(s)	820, 957, 994, 1036, 1174
BssSI	CACGAG	2 cut(s)	354, 512
BssT1I	CCWWGG	3 cut(s)	210, 1230, 1240
Bst2BI	CACGAG	2 cut(s)	354, 512
Bst2UI	CCWGG	2 cut(s)	498, 807
Bst4CI	ACNGT	6 cut(s)	67, 575, 601, 644, 1157, 1254
BstC8I	GCNNGC	1 cut(s)	183
BstDEI	CTNAG	3 cut(s)	460, 900, 1275
BstDSI	CCRYGG	4 cut(s)	210, 1103, 1230, 1240
BstF5I	GGATG	5 cut(s)	57, 682, 868, 1112, 1250
BstKTI	GATC	5 cut(s)	823, 960, 997, 1039, 1177
BstMAI	GTCTC	4 cut(s)	163, 302, 766, 941
BstMBI	GATC	5 cut(s)	820, 957, 994, 1036, 1174
BstMWI	GCNNNNNNNGC	2 cut(s)	350, 797
BstNI	CCWGG	2 cut(s)	498, 807
BstNSI	RCATGY	1 cut(s)	832
BstSCI	CCNGG	2 cut(s)	496, 805
BstSFI	CTRYAG	2 cut(s)	36, 1069
BstSLI	GKGCMC	3 cut(s)	381, 432, 1059
BstV1I	GCAGC	3 cut(s)	22, 495, 526
BstV2I	GAAGAC	1 cut(s)	1140
BstX2I	RGATCY	3 cut(s)	994, 1036, 1174
BstYI	RGATCY	3 cut(s)	994, 1036, 1174
BsuI	GTATCC	1 cut(s)	1154
BsuRI	GGCC	3 cut(s)	893, 1057, 1101
BtgI	CCRYGG	4 cut(s)	210, 1103, 1230, 1240
BtgZI	GCGATG	2 cut(s)	45, 946
BtsCI	GGATG	5 cut(s)	57, 682, 868, 1112, 1250
BtsIMutI	CAGTG	5 cut(s)	291, 626, 1126, 1162, 1193
Cac8I	GCNNGC	1 cut(s)	183
CciI	TCATGA	1 cut(s)	615
Cfr13I	GGNCC	4 cut(s)	891, 1055, 1056, 1100
CsiI	ACCWGGT	1 cut(s)	805
Csp6I	GTAC	1 cut(s)	472
CviAII	CATG	9 cut(s)	211, 493, 543, 616, 747, 775, 829, 1231, 1241
CviQI	GTAC	1 cut(s)	472
DdeI	CTNAG	3 cut(s)	460, 900, 1275
DpnI	GATC	5 cut(s)	822, 959, 996, 1038, 1176
DpnII	GATC	5 cut(s)	820, 957, 994, 1036, 1174
DraIII	CACNNNGTG	1 cut(s)	1004
Eco130I	CCWWGG	3 cut(s)	210, 1230, 1240
Eco24I	GRGCYC	1 cut(s)	1059
Eco31I	GGTCTC	2 cut(s)	302, 766
Eco32I	GATATC	1 cut(s)	608
Eco57I	CTGAAG	1 cut(s)	1217
Eco88I	CYCGRG	1 cut(s)	621
EcoO109I	RGGNCCY	1 cut(s)	1056
EcoRII	CCWGG	2 cut(s)	496, 805
EcoRV	GATATC	1 cut(s)	608
EcoT14I	CCWWGG	3 cut(s)	210, 1230, 1240
EcoT38I	GRGCYC	1 cut(s)	1059
ErhI	CCWWGG	3 cut(s)	210, 1230, 1240
Esp3I	CGTCTC	1 cut(s)	163
FaeI	CATG	9 cut(s)	214, 496, 546, 619, 750, 778, 832, 1234, 1244
FalI	AAGNNNNNCTT	4 cut(s)	984, 1016, 1023, 1055
FaqI	GGGAC	1 cut(s)	630
FatI	CATG	9 cut(s)	210, 492, 542, 615, 746, 774, 828, 1230, 1240
FauNDI	CATATG	1 cut(s)	887
Fnu4HI	GCNGC	4 cut(s)	28, 36, 484, 540
FokI	GGATG	5 cut(s)	64, 669, 875, 1099, 1257
FriOI	GRGCYC	1 cut(s)	1059
Fsp4HI	GCNGC	4 cut(s)	28, 36, 484, 540
FspBI	CTAG	2 cut(s)	374, 449
GluI	GCNGC	4 cut(s)	28, 36, 484, 540
GsaI	CCCAGC	1 cut(s)	1056
GsuI	CTGGAG	2 cut(s)	739, 748
HaeIII	GGCC	3 cut(s)	893, 1057, 1101
Hin1II	CATG	9 cut(s)	214, 496, 546, 619, 750, 778, 832, 1234, 1244
HincII	GTYRAC	3 cut(s)	325, 988, 1093
HindII	GTYRAC	3 cut(s)	325, 988, 1093
HinfI	GANTC	4 cut(s)	202, 229, 453, 1235
HpaI	GTTAAC	1 cut(s)	325
HphI	GGTGA	4 cut(s)	16, 79, 84, 117
Hpy166II	GTNNAC	6 cut(s)	118, 325, 430, 503, 988, 1093
Hpy188I	TCNGA	4 cut(s)	285, 463, 710, 742
Hpy188III	TCNNGA	6 cut(s)	356, 512, 616, 765, 824, 1040
Hpy8I	GTNNAC	6 cut(s)	118, 325, 430, 503, 988, 1093
HpyAV	CCTTC	2 cut(s)	920, 1025
HpyCH4III	ACNGT	6 cut(s)	67, 575, 601, 644, 1157, 1254
HpyCH4V	TGCA	8 cut(s)	38, 243, 430, 467, 483, 542, 682, 1248
HpyF10VI	GCNNNNNNNGC	2 cut(s)	350, 797
HpyF3I	CTNAG	3 cut(s)	460, 900, 1275
Hsp92II	CATG	9 cut(s)	214, 496, 546, 619, 750, 778, 832, 1234, 1244
KspAI	GTTAAC	1 cut(s)	325
Kzo9I	GATC	5 cut(s)	820, 957, 994, 1036, 1174
LmnI	GCTCC	3 cut(s)	593, 910, 1183
Lsp1109I	GCAGC	3 cut(s)	22, 495, 526
LweI	GCATC	7 cut(s)	22, 268, 414, 454, 514, 691, 1235
MabI	ACCWGGT	1 cut(s)	805
MaeI	CTAG	2 cut(s)	374, 449
MaeIII	GTNAC	3 cut(s)	40, 199, 509
MalI	GATC	5 cut(s)	822, 959, 996, 1038, 1176
MboI	GATC	5 cut(s)	820, 957, 994, 1036, 1174
MboII	GAAGA	2 cut(s)	1145, 1210
MflI	RGATCY	3 cut(s)	994, 1036, 1174
MhlI	GDGCHC	5 cut(s)	381, 432, 628, 1059, 1188
MluCI	AATT	2 cut(s)	246, 531
MlyI	GAGTC	2 cut(s)	196, 1229
MmeI	TCCRAC	4 cut(s)	206, 688, 794, 1192
MroXI	GAANNNNTTC	1 cut(s)	233
MseI	TTAA	3 cut(s)	107, 324, 443
MspA1I	CMGCKG	1 cut(s)	717
MspR9I	CCNGG	2 cut(s)	498, 807
MvaI	CCWGG	2 cut(s)	498, 807
MwoI	GCNNNNNNNGC	2 cut(s)	350, 797
NcoI	CCATGG	3 cut(s)	210, 1230, 1240
NdeI	CATATG	1 cut(s)	887
NdeII	GATC	5 cut(s)	820, 957, 994, 1036, 1174
NlaIII	CATG	9 cut(s)	214, 496, 546, 619, 750, 778, 832, 1234, 1244
NlaIV	GGNNCC	4 cut(s)	589, 906, 1057, 1058
NmuCI	GTSAC	2 cut(s)	199, 509
NspI	RCATGY	1 cut(s)	832
PagI	TCATGA	1 cut(s)	615
PdmI	GAANNNNTTC	1 cut(s)	233
PfeI	GAWTC	2 cut(s)	229, 453
PflMI	CCANNNNNTGG	1 cut(s)	216
PkrI	GCNGC	4 cut(s)	29, 37, 485, 541
PleI	GAGTC	2 cut(s)	196, 1229
PpsI	GAGTC	2 cut(s)	196, 1229
Psp6I	CCWGG	2 cut(s)	496, 805
PspFI	CCCAGC	1 cut(s)	1052
PspGI	CCWGG	2 cut(s)	496, 805
PspN4I	GGNNCC	4 cut(s)	589, 906, 1057, 1058
PspOMI	GGGCCC	1 cut(s)	1055
PspPI	GGNCC	4 cut(s)	891, 1055, 1056, 1100
PsrI	GAACNNNNNNTAC	2 cut(s)	1102, 1134
PstI	CTGCAG	1 cut(s)	40
PsuI	RGATCY	3 cut(s)	994, 1036, 1174
PvuII	CAGCTG	1 cut(s)	717
RsaI	GTAC	1 cut(s)	473
RsaNI	GTAC	1 cut(s)	472
SaqAI	TTAA	3 cut(s)	107, 324, 443
SatI	GCNGC	4 cut(s)	28, 36, 484, 540
Sau3AI	GATC	5 cut(s)	820, 957, 994, 1036, 1174
Sau96I	GGNCC	4 cut(s)	891, 1055, 1056, 1100
SchI	GAGTC	2 cut(s)	196, 1229
ScrFI	CCNGG	2 cut(s)	498, 807
SduI	GDGCHC	5 cut(s)	381, 432, 628, 1059, 1188
SexAI	ACCWGGT	1 cut(s)	805
SfaNI	GCATC	7 cut(s)	22, 268, 414, 454, 514, 691, 1235
SfcI	CTRYAG	2 cut(s)	36, 1069
SmlI	CTYRAG	2 cut(s)	833, 1040
SmoI	CTYRAG	2 cut(s)	833, 1040
Sse9I	AATT	2 cut(s)	246, 531
SsiI	CCGC	2 cut(s)	27, 419
SspMI	CTAG	2 cut(s)	374, 449
StyD4I	CCNGG	2 cut(s)	496, 805
StyI	CCWWGG	3 cut(s)	210, 1230, 1240
TaaI	ACNGT	6 cut(s)	67, 575, 601, 644, 1157, 1254
TasI	AATT	2 cut(s)	246, 531
TauI	GCSGC	1 cut(s)	30
TfiI	GAWTC	2 cut(s)	229, 453
Tru1I	TTAA	3 cut(s)	107, 324, 443
Tru9I	TTAA	3 cut(s)	107, 324, 443
TscAI	CASTG	5 cut(s)	298, 633, 1133, 1162, 1200
TseFI	GTSAC	2 cut(s)	199, 509
TseI	GCWGC	3 cut(s)	35, 483, 539
Tsp45I	GTSAC	2 cut(s)	199, 509
TspDTI	ATGAA	5 cut(s)	76, 647, 791, 795, 879
TspRI	CASTG	5 cut(s)	298, 633, 1133, 1162, 1200
Van91I	CCANNNNNTGG	1 cut(s)	216
VneI	GTGCAC	1 cut(s)	428
XapI	RAATTY	1 cut(s)	531
XceI	RCATGY	1 cut(s)	832
XcmI	CCANNNNNNNNNTGG	1 cut(s)	998
XmnI	GAANNNNTTC	1 cut(s)	233
XspI	CTAG	2 cut(s)	374, 449

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.