Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003741

Physical Location & Seq

Genomic Coordinates Forward (+)

962468 .. 964449

Gene Structure 1982 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1012600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 867 bp

ATGCAGGATAACACGACTAACTCTCGTACTTCACTTGCAGCAAAACCATGCCTGGTTCACTTGGCCACGAAGAACAAGATGCCAAAACATTTGCTGCTTGGGTTCGATATATATGTTGTAACTCAATCAAAAGGGGGAATTGATTACTTGAAGTATGATAACTGTTATAACGATGAATCCAAGCCAACTGTTAGGTTCCCTGTAATGACCCGAGCTCTGATGAAAGCAGGTCGTCCCATATTCTATTCGCTTTGTGAATGGGGAGATATGCACCCTGCTACATGGGGTGCTAATGTAGGAAATAGCTGGAGAACTACTAGTGACATTTCTGATACATGGGAAAGTATGGTCTCTAGAGCAGACATGAATGAAGTTTATGCTGAATTTGCCAGACCTGGTGGCTGGAATGATCCCGACATGTTAGAAGTGGGGAATGGAGGAATGAGAAAAGATGAATATATAGTCCATTTTAGCATATGGGCCATTTCTAAGGCTCCCCTTCTTCTCGGTTGCAACGTGGGGAATATCACGAAAGAGACCATGGATATCATTGCAAATAAAGAGGTTATCTCCGTAAACCAAGATCCACTCGGTGTTCAAGCTAAAAAGGTCAGATTACAAGGGAATCGTGAGGTTTGGGCAGGGCCCCTCTCAGGATACAGAGTAGCTTTACTCCTTGTCAACCGAAGCCGTAAGCGAGATTCGTTCACAGCTCACTGGGACGACATTGGGATCCCCACAAACAGTGTTGTTGAAGCAAGAAACCTCTGGGAGCACAAGACATTGAAGGCACGATTTGTTGGAAACTTGACAACCACGCTGGACTCTCATGCATGTAAAATGTATGTTCTGAAGCCGGTGGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0048046 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603

Pfam Domains

Protein Families

Melibiase_2 Melibiase_2_C

Protein Analysis

289

Amino Acids

32.31

Weight (kDa)

8.86

Isoelectric Point (pI)

28.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	35 - 195	4.7e-32	Alpha galactosidase A
Melibiase_C	PF17801	207 - 284	2e-16	Alpha galactosidase C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003741)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08380 AT5G08380
fragaria_vesca	FvH4_2g12370 FvH4_2g12370 FvH4_2g12370 FvH4_4g37361
malus_domestica	MD13G1000400.v1.1 MD16G1012600.v1.1
prunus_persica	Prupe.1G352200_v2.0.a1 Prupe.1G352200_v2.0.a1
pyrus_communis	pycom13g00040 pycom16g01180 pycom2749g00030
rosa_chinensis	RchiOBHm_Chr4g0447241 RchiOBHm_Chr6g0272391
rosa_laevigata	RLG00000005594 RLG00000013676
rosa_multiflora	Rmu_co8492007.1_g000001 Rmu_sc0018949.1_g000001 Rmu_sc0018950.1_g000001
rosa_roxburghii	Rroxscaffold_5G00387600 Rroxscaffold_7G00196300
rosa_rugosa	Rorug04G0379000 Rorug06G0069000
rosa_samantha	Rh4AG440500 Rh4BG449500 Rh4CG466600 Rh4DG448000 Rh6AG184900 Rh6BG188200 Rh6CG186100 Rh6DG177900
rosa_wichuraiana	Rw4G037690 Rw6G015990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	168
Acc36I	ACCTGC	1 cut(s)	218
AclWI	GGATC	4 cut(s)	404, 578, 727, 740
AcoI	YGGCCR	1 cut(s)	63
AcsI	RAATTY	1 cut(s)	383
AdeI	CACNNNGTG	1 cut(s)	593
AfaI	GTAC	1 cut(s)	28
AfiI	CCNNNNNNNGG	1 cut(s)	653
AflIII	ACRYGT	1 cut(s)	417
AgsI	TTSAA	4 cut(s)	151, 599, 755, 787
AhlI	ACTAGT	1 cut(s)	317
AjnI	CCWGG	2 cut(s)	51, 394
AluBI	AGCT	5 cut(s)	215, 306, 602, 668, 713
AluI	AGCT	5 cut(s)	215, 306, 602, 668, 713
Alw21I	GWGCWC	2 cut(s)	217, 777
Alw26I	GTCTC	2 cut(s)	355, 530
AlwI	GGATC	4 cut(s)	404, 578, 727, 740
Ama87I	CYCGRG	1 cut(s)	210
AoxI	GGCC	3 cut(s)	63, 480, 644
ApaI	GGGCCC	1 cut(s)	648
ApeKI	GCWGC	2 cut(s)	38, 94
ApoI	RAATTY	1 cut(s)	383
AspS9I	GGNCC	3 cut(s)	480, 644, 645
AvaI	CYCGRG	1 cut(s)	210
BaeGI	GKGCMC	1 cut(s)	648
BalI	TGGCCA	1 cut(s)	65
BamHI	GGATCC	1 cut(s)	732
BanII	GRGCYC	2 cut(s)	217, 648
Bbv12I	GWGCWC	2 cut(s)	217, 777
BbvI	GCAGC	2 cut(s)	50, 81
BceAI	ACGGC	1 cut(s)	675
BciT130I	CCWGG	2 cut(s)	53, 396
BciVI	GTATCC	1 cut(s)	650
BcoDI	GTCTC	2 cut(s)	355, 530
BcuI	ACTAGT	1 cut(s)	317
BfaI	CTAG	3 cut(s)	318, 354, 865
BfuAI	ACCTGC	1 cut(s)	218
BfuI	GTATCC	1 cut(s)	650
BisI	GCNGC	2 cut(s)	39, 95
BlsI	GCNGC	2 cut(s)	40, 96
Bme1390I	CCNGG	2 cut(s)	53, 396
BmeT110I	CYCGRG	1 cut(s)	210
BmgT120I	GGNCC	3 cut(s)	480, 644, 645
BmiI	GGNNCC	5 cut(s)	197, 495, 646, 647, 734
BmrFI	CCNGG	2 cut(s)	53, 396
BmrI	ACTGGG	1 cut(s)	727
BmsI	GCATC	1 cut(s)	69
BmuI	ACTGGG	1 cut(s)	727
BplI	GAGNNNNNCTC	2 cut(s)	554, 586
BpmI	CTGGAG	1 cut(s)	328
BsaI	GGTCTC	2 cut(s)	355, 530
BsaJI	CCNNGG	1 cut(s)	540
Bsc4I	CCNNNNNNNGG	1 cut(s)	653
Bse118I	RCCGGY	1 cut(s)	856
Bse1I	ACTGG	1 cut(s)	722
Bse3DI	GCAATG	1 cut(s)	549
BseBI	CCWGG	2 cut(s)	53, 396
BseDI	CCNNGG	1 cut(s)	540
BseLI	CCNNNNNNNGG	1 cut(s)	653
BseMI	GCAATG	1 cut(s)	549
BseMII	CTCAG	1 cut(s)	666
BseNI	ACTGG	1 cut(s)	722
BseSI	GKGCMC	1 cut(s)	648
BseXI	GCAGC	2 cut(s)	50, 81
BshFI	GGCC	3 cut(s)	65, 482, 646
BsiHKAI	GWGCWC	2 cut(s)	217, 777
BsiHKCI	CYCGRG	1 cut(s)	210
BsiSI	CCGG	1 cut(s)	857
BslFI	GGGAC	2 cut(s)	219, 734
BslI	CCNNNNNNNGG	1 cut(s)	653
BsmAI	GTCTC	2 cut(s)	355, 530
BsmFI	GGGAC	2 cut(s)	219, 734
BsnI	GGCC	3 cut(s)	65, 482, 646
Bso31I	GGTCTC	2 cut(s)	355, 530
BsoBI	CYCGRG	1 cut(s)	210
Bsp120I	GGGCCC	1 cut(s)	644
Bsp1286I	GDGCHC	3 cut(s)	217, 648, 777
Bsp143I	GATC	3 cut(s)	409, 583, 732
Bsp19I	CCATGG	1 cut(s)	540
BspANI	GGCC	3 cut(s)	65, 482, 646
BspCNI	CTCAG	1 cut(s)	665
BspLI	GGNNCC	5 cut(s)	197, 495, 646, 647, 734
BspMI	ACCTGC	1 cut(s)	218
BspPI	GGATC	4 cut(s)	404, 578, 727, 740
BspTNI	GGTCTC	2 cut(s)	355, 530
BsrDI	GCAATG	1 cut(s)	549
BsrFI	RCCGGY	1 cut(s)	856
BsrI	ACTGG	1 cut(s)	722
BssAI	RCCGGY	1 cut(s)	856
BssECI	CCNNGG	1 cut(s)	540
BssMI	GATC	3 cut(s)	409, 583, 732
BssT1I	CCWWGG	1 cut(s)	540
Bst2UI	CCWGG	2 cut(s)	53, 396
Bst4CI	ACNGT	3 cut(s)	164, 190, 746
BstDEI	CTNAG	2 cut(s)	489, 652
BstDSI	CCRYGG	1 cut(s)	540
BstKTI	GATC	3 cut(s)	412, 586, 735
BstMAI	GTCTC	2 cut(s)	355, 530
BstMBI	GATC	3 cut(s)	409, 583, 732
BstMWI	GCNNNNNNNGC	1 cut(s)	386
BstNI	CCWGG	2 cut(s)	53, 396
BstNSI	RCATGY	2 cut(s)	421, 837
BstSCI	CCNGG	2 cut(s)	51, 394
BstSLI	GKGCMC	1 cut(s)	648
BstV1I	GCAGC	2 cut(s)	50, 81
BstX2I	RGATCY	2 cut(s)	583, 732
BstYI	RGATCY	2 cut(s)	583, 732
BsuI	GTATCC	1 cut(s)	650
BsuRI	GGCC	3 cut(s)	65, 482, 646
BtgI	CCRYGG	1 cut(s)	540
BtsIMutI	CAGTG	2 cut(s)	715, 751
BveI	ACCTGC	1 cut(s)	218
Cfr10I	RCCGGY	1 cut(s)	856
Cfr13I	GGNCC	3 cut(s)	480, 644, 645
CsiI	ACCWGGT	1 cut(s)	394
Csp6I	GTAC	1 cut(s)	27
CviAII	CATG	8 cut(s)	48, 282, 336, 364, 418, 541, 830, 834
CviQI	GTAC	1 cut(s)	27
DdeI	CTNAG	2 cut(s)	489, 652
DpnI	GATC	3 cut(s)	411, 585, 734
DpnII	GATC	3 cut(s)	409, 583, 732
DraIII	CACNNNGTG	1 cut(s)	593
EaeI	YGGCCR	1 cut(s)	63
Ecl136II	GAGCTC	1 cut(s)	215
Eco130I	CCWWGG	1 cut(s)	540
Eco24I	GRGCYC	2 cut(s)	217, 648
Eco31I	GGTCTC	2 cut(s)	355, 530
Eco32I	GATATC	1 cut(s)	547
Eco53kI	GAGCTC	1 cut(s)	215
Eco88I	CYCGRG	1 cut(s)	210
EcoICRI	GAGCTC	1 cut(s)	215
EcoO109I	RGGNCCY	2 cut(s)	644, 645
EcoRII	CCWGG	2 cut(s)	51, 394
EcoRV	GATATC	1 cut(s)	547
EcoT14I	CCWWGG	1 cut(s)	540
EcoT22I	ATGCAT	1 cut(s)	835
EcoT38I	GRGCYC	2 cut(s)	217, 648
ErhI	CCWWGG	1 cut(s)	540
FaeI	CATG	8 cut(s)	51, 285, 339, 367, 421, 544, 833, 837
FaqI	GGGAC	2 cut(s)	219, 734
FatI	CATG	8 cut(s)	47, 281, 335, 363, 417, 540, 829, 833
FauNDI	CATATG	1 cut(s)	476
Fnu4HI	GCNGC	2 cut(s)	39, 95
FriOI	GRGCYC	2 cut(s)	217, 648
Fsp4HI	GCNGC	2 cut(s)	39, 95
FspBI	CTAG	3 cut(s)	318, 354, 865
GluI	GCNGC	2 cut(s)	39, 95
GsuI	CTGGAG	1 cut(s)	328
HaeIII	GGCC	3 cut(s)	65, 482, 646
HapII	CCGG	1 cut(s)	857
Hin1II	CATG	8 cut(s)	51, 285, 339, 367, 421, 544, 833, 837
HincII	GTYRAC	1 cut(s)	682
HindII	GTYRAC	1 cut(s)	682
HinfI	GANTC	4 cut(s)	176, 625, 701, 824
HpaII	CCGG	1 cut(s)	857
Hpy166II	GTNNAC	4 cut(s)	58, 577, 682, 708
Hpy188I	TCNGA	4 cut(s)	219, 331, 614, 852
Hpy188III	TCNNGA	5 cut(s)	354, 413, 529, 629, 654
Hpy8I	GTNNAC	4 cut(s)	58, 577, 682, 708
HpyAV	CCTTC	2 cut(s)	509, 781
HpyCH4III	ACNGT	3 cut(s)	164, 190, 746
HpyCH4IV	ACGT	1 cut(s)	516
HpyCH4V	TGCA	6 cut(s)	4, 38, 271, 513, 554, 833
HpyF10VI	GCNNNNNNNGC	1 cut(s)	386
HpyF3I	CTNAG	2 cut(s)	489, 652
HpySE526I	ACGT	1 cut(s)	516
Hsp92II	CATG	8 cut(s)	51, 285, 339, 367, 421, 544, 833, 837
Kzo9I	GATC	3 cut(s)	409, 583, 732
LmnI	GCTCC	2 cut(s)	499, 772
Lsp1109I	GCAGC	2 cut(s)	50, 81
LweI	GCATC	1 cut(s)	69
MabI	ACCWGGT	1 cut(s)	394
MaeI	CTAG	3 cut(s)	318, 354, 865
MaeII	ACGT	1 cut(s)	516
MaeIII	GTNAC	2 cut(s)	118, 320
MalI	GATC	3 cut(s)	411, 585, 734
MboI	GATC	3 cut(s)	409, 583, 732
MboII	GAAGA	2 cut(s)	82, 494
MflI	RGATCY	2 cut(s)	583, 732
MhlI	GDGCHC	3 cut(s)	217, 648, 777
MlsI	TGGCCA	1 cut(s)	65
MluCI	AATT	2 cut(s)	138, 383
MluNI	TGGCCA	1 cut(s)	65
MlyI	GAGTC	1 cut(s)	818
MmeI	TCCRAC	1 cut(s)	781
MnlI	CCTC	5 cut(s)	431, 556, 625, 659, 776
Mox20I	TGGCCA	1 cut(s)	65
Mph1103I	ATGCAT	1 cut(s)	835
MscI	TGGCCA	1 cut(s)	65
Msp20I	TGGCCA	1 cut(s)	65
MspI	CCGG	1 cut(s)	857
MspR9I	CCNGG	2 cut(s)	53, 396
MvaI	CCWGG	2 cut(s)	53, 396
MwoI	GCNNNNNNNGC	1 cut(s)	386
NcoI	CCATGG	1 cut(s)	540
NdeI	CATATG	1 cut(s)	476
NdeII	GATC	3 cut(s)	409, 583, 732
NlaIII	CATG	8 cut(s)	51, 285, 339, 367, 421, 544, 833, 837
NlaIV	GGNNCC	5 cut(s)	197, 495, 646, 647, 734
NmuCI	GTSAC	1 cut(s)	320
NsiI	ATGCAT	1 cut(s)	835
NspI	RCATGY	2 cut(s)	421, 837
PciI	ACATGT	1 cut(s)	417
PcsI	WCGNNNNNNNCGW	1 cut(s)	513
PfeI	GAWTC	3 cut(s)	176, 625, 701
PkrI	GCNGC	2 cut(s)	40, 96
PleI	GAGTC	1 cut(s)	818
PpsI	GAGTC	1 cut(s)	818
PscI	ACATGT	1 cut(s)	417
PsiI	TTATAA	1 cut(s)	168
Psp124BI	GAGCTC	1 cut(s)	217
Psp6I	CCWGG	2 cut(s)	51, 394
PspGI	CCWGG	2 cut(s)	51, 394
PspN4I	GGNNCC	5 cut(s)	197, 495, 646, 647, 734
PspOMI	GGGCCC	1 cut(s)	644
PspPI	GGNCC	3 cut(s)	480, 644, 645
PsuI	RGATCY	2 cut(s)	583, 732
RsaI	GTAC	1 cut(s)	28
RsaNI	GTAC	1 cut(s)	27
SacI	GAGCTC	1 cut(s)	217
SatI	GCNGC	2 cut(s)	39, 95
Sau3AI	GATC	3 cut(s)	409, 583, 732
Sau96I	GGNCC	3 cut(s)	480, 644, 645
SchI	GAGTC	1 cut(s)	818
ScrFI	CCNGG	2 cut(s)	53, 396
SduI	GDGCHC	3 cut(s)	217, 648, 777
SexAI	ACCWGGT	1 cut(s)	394
SfaNI	GCATC	1 cut(s)	69
SpeI	ACTAGT	1 cut(s)	317
Sse9I	AATT	2 cut(s)	138, 383
SspMI	CTAG	3 cut(s)	318, 354, 865
SstI	GAGCTC	1 cut(s)	217
StyD4I	CCNGG	2 cut(s)	51, 394
StyI	CCWWGG	1 cut(s)	540
TaaI	ACNGT	3 cut(s)	164, 190, 746
TaiI	ACGT	1 cut(s)	519
TaqI	TCGA	1 cut(s)	105
TasI	AATT	2 cut(s)	138, 383
TfiI	GAWTC	3 cut(s)	176, 625, 701
TscAI	CASTG	2 cut(s)	722, 751
TseFI	GTSAC	1 cut(s)	320
TseI	GCWGC	2 cut(s)	38, 94
Tsp45I	GTSAC	1 cut(s)	320
TspDTI	ATGAA	5 cut(s)	189, 236, 380, 384, 468
TspGWI	ACGGA	1 cut(s)	562
TspRI	CASTG	2 cut(s)	722, 751
XapI	RAATTY	1 cut(s)	383
XbaI	TCTAGA	1 cut(s)	353
XceI	RCATGY	2 cut(s)	421, 837
XspI	CTAG	3 cut(s)	318, 354, 865
Zsp2I	ATGCAT	1 cut(s)	835

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.