Rh4DG448000

Alpha-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003741
Physical Location & Seq
Forward (+)
64685210 .. 64689170
3961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG448000.1

Sequence Viewer

Length: 1044 bp
ATGGTGAATATGAGGGGGAAAAGTGGCGGTGATGCTGCAGTAACAACGAGGATGATTGTAATGACGGTGATGATTGTATGCTCATTCATATCACCACAACCGATGTTAATGGGTGTTTGCGCTTCACCGATAACAAGAATGATGGTGGCAGACACCAAAGAGCAACAGAGACGAAATCTGCTTGCTAATGGGCTTGGAGTGACTCCTCCCATGGGGTGGAATAGTTGGAATCACTTCAACTGCAAAATTGATGAGAAAATCATCAAAGCAACTGCTGATGCTCTGATTTCCACTGGTCTCTCTAAACTTGGATATACCTATGTTAACATAGATGATTGTTGGGCAGAAGTAGCTCGTGATGACAAGGGTAATCTAGTGCCCAAGAAATCCACATTTCCATCTGGCATCAAAGCTCTTGCGGATTATGTGCACAGCAAGGGACTAAAGCTAGGAATCTACTCAGATGCAGGGTACTTTACTTGCAGCAAAACCATGCCTGGTTCACTTGGTCACGAGGAACAAGATGCCAAAATTTTTGCTGCATGGGGTATTGATTATTTGAAGTATGACAACTGTAATACTGATGGCTCCAAACCAACTGTTAGATATCCTGTCATGACTCGAGCACTGATGAAAGCAGACCGTCCCATATTCTTTTCTCTGTGTGAATGGGGAGATTTGCATCCTGCTTTATGGGGTGCTAAAGTCGGAAACAGCTGGAGAACTACTAATGACATTTCTGATACATGGGAAAGTATGGTCTCCAGAGCAGACATGAATGAAGTTTATGCTGAATATGCTAGACCTGGTGGTTGGAATGATCCTGACATGCTTGAGGTGGGAAATGGAGGAATGACAAAGGATGAATATGTAGTCCATTTTAGCATATGGGCCATATCTAAGGCTCCCCTTCTTATTGGTTGTGATGTGAGGAATATGACAAAAGAGACTATGGAGATCATCGCAAATAAAGAGGTCATCTCTGTCAACCAAGGTAGTTACGACCTTTGGCTGAAAAAGAAAAACAATTTGTTTCAGTTATAG

Protein Analysis

347

Amino Acids

38.61

Weight (kDa)

6.94

Isoelectric Point (pI)

20.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Melibiase_2 PF16499 68 - 331 5e-74 Alpha galactosidase A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 216
AciI CCGC 2 cut(s) 27, 419
AclWI GGATC 1 cut(s) 815
AcsI RAATTY 1 cut(s) 531
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 2 cut(s) 212, 216
AgsI TTSAA 2 cut(s) 238, 562
AjnI CCWGG 2 cut(s) 496, 805
AluBI AGCT 4 cut(s) 353, 413, 448, 717
AluI AGCT 4 cut(s) 353, 413, 448, 717
Alw21I GWGCWC 2 cut(s) 432, 628
Alw26I GTCTC 4 cut(s) 163, 302, 766, 941
Alw44I GTGCAC 1 cut(s) 428
AlwI GGATC 1 cut(s) 815
Ama87I CYCGRG 1 cut(s) 621
AoxI GGCC 1 cut(s) 891
ApaLI GTGCAC 1 cut(s) 428
ApeKI GCWGC 3 cut(s) 35, 483, 539
ApoI RAATTY 1 cut(s) 531
Asp700I GAANNNNTTC 1 cut(s) 233
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 891
AsuHPI GGTGA 5 cut(s) 16, 41, 79, 84, 117
AvaI CYCGRG 1 cut(s) 621
BaeGI GKGCMC 2 cut(s) 381, 432
BauI CACGAG 2 cut(s) 354, 512
Bbv12I GWGCWC 2 cut(s) 432, 628
BbvI GCAGC 3 cut(s) 22, 495, 526
BccI CCATC 3 cut(s) 136, 406, 578
BciT130I CCWGG 2 cut(s) 498, 807
BcoDI GTCTC 4 cut(s) 163, 302, 766, 941
BfaI CTAG 3 cut(s) 374, 449, 801
BfmI CTRYAG 1 cut(s) 36
BisI GCNGC 3 cut(s) 36, 484, 540
BlsI GCNGC 3 cut(s) 37, 485, 541
Bme1390I CCNGG 2 cut(s) 498, 807
BmeT110I CYCGRG 1 cut(s) 621
BmgT120I GGNCC 1 cut(s) 891
BmiI GGNNCC 2 cut(s) 589, 906
BmrFI CCNGG 2 cut(s) 498, 807
BmsI GCATC 6 cut(s) 22, 268, 414, 454, 514, 691
BplI GAGNNNNNCTC 2 cut(s) 965, 997
BpmI CTGGAG 2 cut(s) 739, 748
BpuEI CTTGAG 1 cut(s) 854
BsaBI GATNNNNATC 1 cut(s) 681
BsaI GGTCTC 2 cut(s) 302, 766
BsaJI CCNNGG 2 cut(s) 210, 991
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 216
Bse1I ACTGG 1 cut(s) 298
Bse8I GATNNNNATC 1 cut(s) 681
BseBI CCWGG 2 cut(s) 498, 807
BseDI CCNNGG 2 cut(s) 210, 991
BseGI GGATG 3 cut(s) 57, 682, 868
BseJI GATNNNNATC 1 cut(s) 681
BseLI CCNNNNNNNGG 2 cut(s) 212, 216
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 1 cut(s) 298
BseRI GAGGAG 1 cut(s) 195
BseSI GKGCMC 2 cut(s) 381, 432
BseXI GCAGC 3 cut(s) 22, 495, 526
BshFI GGCC 1 cut(s) 893
BsiHKAI GWGCWC 2 cut(s) 432, 628
BsiHKCI CYCGRG 1 cut(s) 621
BslFI GGGAC 2 cut(s) 453, 630
BslI CCNNNNNNNGG 2 cut(s) 212, 216
BsmAI GTCTC 4 cut(s) 163, 302, 766, 941
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 2 cut(s) 453, 630
BsnI GGCC 1 cut(s) 893
Bso31I GGTCTC 2 cut(s) 302, 766
BsoBI CYCGRG 1 cut(s) 621
Bsp1286I GDGCHC 3 cut(s) 381, 432, 628
Bsp143I GATC 2 cut(s) 820, 957
Bsp19I CCATGG 1 cut(s) 210
BspACI CCGC 2 cut(s) 27, 419
BspANI GGCC 1 cut(s) 893
BspCNI CTCAG 1 cut(s) 473
BspHI TCATGA 1 cut(s) 615
BspLI GGNNCC 2 cut(s) 589, 906
BspMAI CTGCAG 1 cut(s) 40
BspPI GGATC 1 cut(s) 815
BspTNI GGTCTC 2 cut(s) 302, 766
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 2 cut(s) 210, 991
BssMI GATC 2 cut(s) 820, 957
BssSI CACGAG 2 cut(s) 354, 512
BssT1I CCWWGG 2 cut(s) 210, 991
Bst2BI CACGAG 2 cut(s) 354, 512
Bst2UI CCWGG 2 cut(s) 498, 807
Bst4CI ACNGT 4 cut(s) 67, 575, 601, 644
BstC8I GCNNGC 1 cut(s) 183
BstDEI CTNAG 2 cut(s) 460, 900
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 3 cut(s) 57, 682, 868
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 2 cut(s) 823, 960
BstMAI GTCTC 4 cut(s) 163, 302, 766, 941
BstMBI GATC 2 cut(s) 820, 957
BstMWI GCNNNNNNNGC 2 cut(s) 350, 797
BstNI CCWGG 2 cut(s) 498, 807
BstNSI RCATGY 1 cut(s) 832
BstSCI CCNGG 2 cut(s) 496, 805
BstSFI CTRYAG 1 cut(s) 36
BstSLI GKGCMC 2 cut(s) 381, 432
BstV1I GCAGC 3 cut(s) 22, 495, 526
BsuRI GGCC 1 cut(s) 893
BtgI CCRYGG 1 cut(s) 210
BtgZI GCGATG 1 cut(s) 946
BtsCI GGATG 3 cut(s) 57, 682, 868
BtsIMutI CAGTG 2 cut(s) 291, 626
Cac8I GCNNGC 1 cut(s) 183
CciI TCATGA 1 cut(s) 615
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 891
CsiI ACCWGGT 1 cut(s) 805
Csp6I GTAC 1 cut(s) 472
CviAII CATG 7 cut(s) 211, 493, 543, 616, 747, 775, 829
CviJI RGCY 9 cut(s) 193, 353, 413, 448, 588, 717, 893, 905, 1012
CviKI_1 RGCY 9 cut(s) 193, 353, 413, 448, 588, 717, 893, 905, 1012
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 2 cut(s) 460, 900
DpnI GATC 2 cut(s) 822, 959
DpnII GATC 2 cut(s) 820, 957
Eco130I CCWWGG 2 cut(s) 210, 991
Eco31I GGTCTC 2 cut(s) 302, 766
Eco32I GATATC 1 cut(s) 608
Eco88I CYCGRG 1 cut(s) 621
EcoRII CCWGG 2 cut(s) 496, 805
EcoRV GATATC 1 cut(s) 608
EcoT14I CCWWGG 2 cut(s) 210, 991
ErhI CCWWGG 2 cut(s) 210, 991
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 7 cut(s) 214, 496, 546, 619, 750, 778, 832
FaqI GGGAC 2 cut(s) 453, 630
FatI CATG 7 cut(s) 210, 492, 542, 615, 746, 774, 828
FauNDI CATATG 1 cut(s) 887
Fnu4HI GCNGC 3 cut(s) 36, 484, 540
FokI GGATG 3 cut(s) 64, 669, 875
Fsp4HI GCNGC 3 cut(s) 36, 484, 540
FspBI CTAG 3 cut(s) 374, 449, 801
GlaI GCGC 1 cut(s) 121
GluI GCNGC 3 cut(s) 36, 484, 540
GsuI CTGGAG 2 cut(s) 739, 748
HaeIII GGCC 1 cut(s) 893
HhaI GCGC 1 cut(s) 122
Hin1II CATG 7 cut(s) 214, 496, 546, 619, 750, 778, 832
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HincII GTYRAC 2 cut(s) 325, 988
HindII GTYRAC 2 cut(s) 325, 988
HinfI GANTC 4 cut(s) 202, 229, 453, 619
HpaI GTTAAC 1 cut(s) 325
HphI GGTGA 5 cut(s) 16, 41, 79, 84, 117
Hpy166II GTNNAC 4 cut(s) 325, 430, 503, 988
Hpy188I TCNGA 4 cut(s) 285, 463, 710, 742
Hpy188III TCNNGA 5 cut(s) 356, 512, 616, 765, 824
Hpy8I GTNNAC 4 cut(s) 325, 430, 503, 988
HpyAV CCTTC 1 cut(s) 920
HpyCH4III ACNGT 4 cut(s) 67, 575, 601, 644
HpyCH4V TGCA 7 cut(s) 38, 243, 430, 467, 483, 542, 682
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 797
HpyF3I CTNAG 2 cut(s) 460, 900
Hsp92II CATG 7 cut(s) 214, 496, 546, 619, 750, 778, 832
HspAI GCGC 1 cut(s) 120
KspAI GTTAAC 1 cut(s) 325
Kzo9I GATC 2 cut(s) 820, 957
LmnI GCTCC 2 cut(s) 593, 910
Lsp1109I GCAGC 3 cut(s) 22, 495, 526
LweI GCATC 6 cut(s) 22, 268, 414, 454, 514, 691
MabI ACCWGGT 1 cut(s) 805
MaeI CTAG 3 cut(s) 374, 449, 801
MaeIII GTNAC 4 cut(s) 40, 199, 509, 998
MalI GATC 2 cut(s) 822, 959
MboI GATC 2 cut(s) 820, 957
MhlI GDGCHC 3 cut(s) 381, 432, 628
MluCI AATT 3 cut(s) 246, 531, 1027
MlyI GAGTC 2 cut(s) 196, 613
MmeI TCCRAC 3 cut(s) 206, 688, 794
MnlI CCTC 8 cut(s) 6, 42, 216, 508, 829, 842, 924, 967
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 2 cut(s) 107, 324
MspA1I CMGCKG 1 cut(s) 717
MspR9I CCNGG 2 cut(s) 498, 807
MvaI CCWGG 2 cut(s) 498, 807
MwoI GCNNNNNNNGC 2 cut(s) 350, 797
NcoI CCATGG 1 cut(s) 210
NdeI CATATG 1 cut(s) 887
NdeII GATC 2 cut(s) 820, 957
NlaIII CATG 7 cut(s) 214, 496, 546, 619, 750, 778, 832
NlaIV GGNNCC 2 cut(s) 589, 906
NmuCI GTSAC 2 cut(s) 199, 509
NspI RCATGY 1 cut(s) 832
PaeR7I CTCGAG 1 cut(s) 621
PagI TCATGA 1 cut(s) 615
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 2 cut(s) 229, 453
PflMI CCANNNNNTGG 1 cut(s) 216
PkrI GCNGC 3 cut(s) 37, 485, 541
PleI GAGTC 2 cut(s) 196, 613
PpsI GAGTC 2 cut(s) 196, 613
Psp6I CCWGG 2 cut(s) 496, 805
PspGI CCWGG 2 cut(s) 496, 805
PspN4I GGNNCC 2 cut(s) 589, 906
PspPI GGNCC 1 cut(s) 891
PspXI VCTCGAGB 1 cut(s) 621
PstI CTGCAG 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 717
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
SaqAI TTAA 2 cut(s) 107, 324
SatI GCNGC 3 cut(s) 36, 484, 540
Sau3AI GATC 2 cut(s) 820, 957
Sau96I GGNCC 1 cut(s) 891
SchI GAGTC 2 cut(s) 196, 613
ScrFI CCNGG 2 cut(s) 498, 807
SduI GDGCHC 3 cut(s) 381, 432, 628
SexAI ACCWGGT 1 cut(s) 805
SfaNI GCATC 6 cut(s) 22, 268, 414, 454, 514, 691
SfcI CTRYAG 1 cut(s) 36
Sfr274I CTCGAG 1 cut(s) 621
SlaI CTCGAG 1 cut(s) 621
SmlI CTYRAG 2 cut(s) 621, 833
SmoI CTYRAG 2 cut(s) 621, 833
Sse9I AATT 3 cut(s) 246, 531, 1027
SsiI CCGC 2 cut(s) 27, 419
SspMI CTAG 3 cut(s) 374, 449, 801
StyD4I CCNGG 2 cut(s) 496, 805
StyI CCWWGG 2 cut(s) 210, 991
TaaI ACNGT 4 cut(s) 67, 575, 601, 644
TaqI TCGA 1 cut(s) 622
TasI AATT 3 cut(s) 246, 531, 1027
TfiI GAWTC 2 cut(s) 229, 453
Tru1I TTAA 2 cut(s) 107, 324
Tru9I TTAA 2 cut(s) 107, 324
TscAI CASTG 2 cut(s) 298, 633
TseFI GTSAC 2 cut(s) 199, 509
TseI GCWGC 3 cut(s) 35, 483, 539
Tsp45I GTSAC 2 cut(s) 199, 509
TspDTI ATGAA 5 cut(s) 76, 647, 791, 795, 879
TspRI CASTG 2 cut(s) 298, 633
Van91I CCANNNNNTGG 1 cut(s) 216
VneI GTGCAC 1 cut(s) 428
XapI RAATTY 1 cut(s) 531
XceI RCATGY 1 cut(s) 832
XhoI CTCGAG 1 cut(s) 621
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 3 cut(s) 374, 449, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.