Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003741

Physical Location & Seq

Genomic Coordinates Reverse (-)

51922 .. 55991

Gene Structure 4070 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1000400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1248 bp

ATGGGGAAGAAGGATGGTGGTGATTGTGATCATGGTGCACTAGTGACGATGATGACGATGATTGTGATGTTGTTGATAATGGCAATGGTTGTGTCGTTTGCTTCTTCAGCTTCAGCTGCAGCAGCTTCTTCGCAACTCAGACGAAATCTGCTTGCCAACGGACTTGGACTCACTCCTCCCATGGGGTGGAATAGTTGGAATCACTTCAACTGCAAAATCGATGAGAAAATCATCAAGGCAACCGCTGATGCACTGGTTTCCACTGGTCTCTCTAAACTTGGATATACCTATGTTAACATAGATGATTGCTGGGCCGAAATTGCTCGTGATCACAAGCGCAATCTAGTGCCCAAGAAATCAACATTTCCATCAGGCATTAAAGCTCTTGCGGATTATGTTCACAGCAAGGGTCTTAAGCTAGGAATTTACTCAGATGCAGGGTACTTCACTTGCAGCAAAACCATGCCTGGTTCACTTGGCCACGAAGAGCAAGATGCCAAAACATTTGCTGCTTGGGGAATTGATTATTTGAAGTATGATAACTGTAATAATGATGACTCCAAGCCAACTGTTAGGTACCCTGTAATGACCCGAGCTCTGATGAAAGCAGGTCGTCCCATATTCTTTTCGCTTTGTGAATGGGGAGATTTGCACCCTGCTCAATGGGGTGCTAAAGTAGGAAATAGCTGGAGAACTACTAATGACATTTCTGATACATGGGAAAGTATGATCTCTAGGGCCGACATGAATGAAGTTTATGCTGAATTTGCCAGACCTGGTGGCTGGAATGATCCCGACATGTTAGAAGTGGGGAATGGAGGAATGACAAAAGATGAATATGTAGTCCATTTTAGCATATGGGCCATTTCTAAGGCTCCCCTTCTTCTTGGCTGCGATGTGAGGAATATCACAAAAGAGAACATGGAAATCATTGCAAATAAAGAGGTTATCTCTGTAAACCAAGATCCACTTGGTGTCCAAGCTAAAAAGGTCAGATCAGAAGGGGATCTCGAGATTTGGGCGGGGCCCCTTTCAGGATACAGAGTAGCTCTACTCCTTGTCAACCGAGGCCCTTGGCGAACTGCGGTCACAGCTCACTGGGATGACATTGAGATCCCGACAAACAGTGTTGTTGAAGCAAGAGACCTCTGGGAGCACAAGACATTGAAGGCACGATTTGTCGGAAACTTGACAGCCACTGTGGACTCTCATGCATGCAAAATGTATGTTTTGAAGCCAGTGTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0048046 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603

Pfam Domains

Protein Families

Melibiase_2 Melibiase_2_C

Protein Analysis

416

Amino Acids

45.85

Weight (kDa)

6.56

Isoelectric Point (pI)

26.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	58 - 322	4.1e-77	Alpha galactosidase A
Melibiase_C	PF17801	334 - 411	2.2e-17	Alpha galactosidase C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003741)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08380 AT5G08380
fragaria_vesca	FvH4_2g12370 FvH4_2g12370 FvH4_2g12370 FvH4_4g37361
malus_domestica	MD13G1000400.v1.1 MD16G1012600.v1.1
prunus_persica	Prupe.1G352200_v2.0.a1 Prupe.1G352200_v2.0.a1
pyrus_communis	pycom13g00040 pycom16g01180 pycom2749g00030
rosa_chinensis	RchiOBHm_Chr4g0447241 RchiOBHm_Chr6g0272391
rosa_laevigata	RLG00000005594 RLG00000013676
rosa_multiflora	Rmu_co8492007.1_g000001 Rmu_sc0018949.1_g000001 Rmu_sc0018950.1_g000001
rosa_roxburghii	Rroxscaffold_5G00387600 Rroxscaffold_7G00196300
rosa_rugosa	Rorug04G0379000 Rorug06G0069000
rosa_samantha	Rh4AG440500 Rh4BG449500 Rh4CG466600 Rh4DG448000 Rh6AG184900 Rh6BG188200 Rh6CG186100 Rh6DG177900
rosa_wichuraiana	Rw4G037690 Rw6G015990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	599
Acc65I	GGTACC	1 cut(s)	576
AccB1I	GGYRCC	1 cut(s)	576
AccB7I	CCANNNNNTGG	1 cut(s)	186
AciI	CCGC	4 cut(s)	243, 389, 1022, 1085
AclWI	GGATC	4 cut(s)	785, 959, 1014, 1108
AcoI	YGGCCR	1 cut(s)	478
AcsI	RAATTY	2 cut(s)	423, 764
AcuI	CTGAAG	2 cut(s)	90, 96
AdeI	CACNNNGTG	1 cut(s)	974
AfaI	GTAC	2 cut(s)	443, 578
AfiI	CCNNNNNNNGG	3 cut(s)	182, 186, 1034
AflII	CTTAAG	1 cut(s)	413
AflIII	ACRYGT	1 cut(s)	798
AgsI	TTSAA	5 cut(s)	208, 532, 1136, 1168, 1234
AhlI	ACTAGT	1 cut(s)	40
AjnI	CCWGG	2 cut(s)	466, 775
Alw21I	GWGCWC	3 cut(s)	40, 598, 1158
Alw26I	GTCTC	2 cut(s)	272, 1137
Alw44I	GTGCAC	1 cut(s)	36
AlwI	GGATC	4 cut(s)	785, 959, 1014, 1108
AlwNI	CAGNNNCTG	1 cut(s)	1199
Ama87I	CYCGRG	2 cut(s)	591, 1010
AoxI	GGCC	6 cut(s)	312, 478, 738, 861, 1025, 1069
ApaI	GGGCCC	1 cut(s)	1029
ApaLI	GTGCAC	1 cut(s)	36
ApeKI	GCWGC	6 cut(s)	116, 119, 122, 453, 509, 891
ApoI	RAATTY	2 cut(s)	423, 764
Asp700I	GAANNNNTTC	1 cut(s)	203
Asp718I	GGTACC	1 cut(s)	576
AspLEI	GCGC	1 cut(s)	339
AspS9I	GGNCC	6 cut(s)	312, 738, 861, 1025, 1026, 1070
AsuHPI	GGTGA	1 cut(s)	32
AvaI	CYCGRG	2 cut(s)	591, 1010
BaeGI	GKGCMC	3 cut(s)	40, 351, 1029
BalI	TGGCCA	1 cut(s)	480
BanI	GGYRCC	1 cut(s)	576
BanII	GRGCYC	2 cut(s)	598, 1029
BauI	CACGAG	1 cut(s)	324
Bbv12I	GWGCWC	3 cut(s)	40, 598, 1158
BbvI	GCAGC	6 cut(s)	103, 131, 134, 465, 496, 878
BccI	CCATC	2 cut(s)	8, 376
BcgI	CGANNNNNNTGC	2 cut(s)	111, 145
BciT130I	CCWGG	2 cut(s)	468, 777
BciVI	GTATCC	1 cut(s)	1031
BclI	TGATCA	2 cut(s)	28, 328
BcoDI	GTCTC	2 cut(s)	272, 1137
BcuI	ACTAGT	1 cut(s)	40
BfaI	CTAG	4 cut(s)	41, 344, 419, 735
BfmI	CTRYAG	1 cut(s)	117
BfrI	CTTAAG	1 cut(s)	413
BfuAI	ACCTGC	1 cut(s)	599
BfuI	GTATCC	1 cut(s)	1031
BisI	GCNGC	6 cut(s)	117, 120, 123, 454, 510, 892
BlsI	GCNGC	6 cut(s)	118, 121, 124, 455, 511, 893
Bme1390I	CCNGG	2 cut(s)	468, 777
BmeT110I	CYCGRG	2 cut(s)	591, 1010
BmgT120I	GGNCC	6 cut(s)	312, 738, 861, 1025, 1026, 1070
BmiI	GGNNCC	5 cut(s)	578, 876, 1026, 1027, 1028
BmrFI	CCNGG	2 cut(s)	468, 777
BmrI	ACTGGG	1 cut(s)	1108
BmsI	GCATC	3 cut(s)	238, 424, 484
BmuI	ACTGGG	1 cut(s)	1108
BplI	GAGNNNNNCTC	2 cut(s)	935, 967
BpmI	CTGGAG	1 cut(s)	709
Bsa29I	ATCGAT	1 cut(s)	219
BsaBI	GATNNNNATC	1 cut(s)	27
BsaI	GGTCTC	2 cut(s)	272, 1137
BsaJI	CCNNGG	3 cut(s)	180, 1066, 1073
Bsc4I	CCNNNNNNNGG	3 cut(s)	182, 186, 1034
Bse1I	ACTGG	4 cut(s)	258, 268, 1103, 1238
Bse3DI	GCAATG	2 cut(s)	90, 930
Bse8I	GATNNNNATC	1 cut(s)	27
BseBI	CCWGG	2 cut(s)	468, 777
BseCI	ATCGAT	1 cut(s)	219
BseDI	CCNNGG	3 cut(s)	180, 1066, 1073
BseGI	GGATG	2 cut(s)	19, 1108
BseJI	GATNNNNATC	1 cut(s)	27
BseLI	CCNNNNNNNGG	3 cut(s)	182, 186, 1034
BseMI	GCAATG	2 cut(s)	90, 930
BseMII	CTCAG	2 cut(s)	151, 444
BseNI	ACTGG	4 cut(s)	258, 268, 1103, 1238
BseRI	GAGGAG	1 cut(s)	165
BseSI	GKGCMC	3 cut(s)	40, 351, 1029
BseXI	GCAGC	6 cut(s)	103, 131, 134, 465, 496, 878
BseYI	CCCAGC	1 cut(s)	309
BshFI	GGCC	6 cut(s)	314, 480, 740, 863, 1027, 1071
BshNI	GGYRCC	1 cut(s)	576
BshVI	ATCGAT	1 cut(s)	219
BsiHKAI	GWGCWC	3 cut(s)	40, 598, 1158
BsiHKCI	CYCGRG	2 cut(s)	591, 1010
BslFI	GGGAC	1 cut(s)	600
BslI	CCNNNNNNNGG	3 cut(s)	182, 186, 1034
BsmAI	GTCTC	2 cut(s)	272, 1137
BsmFI	GGGAC	1 cut(s)	600
BsnI	GGCC	6 cut(s)	314, 480, 740, 863, 1027, 1071
Bso31I	GGTCTC	2 cut(s)	272, 1137
BsoBI	CYCGRG	2 cut(s)	591, 1010
Bsp120I	GGGCCC	1 cut(s)	1025
Bsp1286I	GDGCHC	5 cut(s)	40, 351, 598, 1029, 1158
Bsp143I	GATC	8 cut(s)	28, 328, 729, 790, 964, 995, 1006, 1113
Bsp19I	CCATGG	1 cut(s)	180
BspACI	CCGC	4 cut(s)	243, 389, 1022, 1085
BspANI	GGCC	6 cut(s)	314, 480, 740, 863, 1027, 1071
BspCNI	CTCAG	2 cut(s)	150, 443
BspDI	ATCGAT	1 cut(s)	219
BspLI	GGNNCC	5 cut(s)	578, 876, 1026, 1027, 1028
BspMAI	CTGCAG	1 cut(s)	121
BspMI	ACCTGC	1 cut(s)	599
BspPI	GGATC	4 cut(s)	785, 959, 1014, 1108
BspQI	GCTCTTC	1 cut(s)	480
BspT107I	GGYRCC	1 cut(s)	576
BspTI	CTTAAG	1 cut(s)	413
BspTNI	GGTCTC	2 cut(s)	272, 1137
BsrDI	GCAATG	2 cut(s)	90, 930
BsrI	ACTGG	4 cut(s)	258, 268, 1103, 1238
BssECI	CCNNGG	3 cut(s)	180, 1066, 1073
BssMI	GATC	8 cut(s)	28, 328, 729, 790, 964, 995, 1006, 1113
BssSI	CACGAG	1 cut(s)	324
BssT1I	CCWWGG	2 cut(s)	180, 1073
Bst2BI	CACGAG	1 cut(s)	324
Bst2UI	CCWGG	2 cut(s)	468, 777
Bst4CI	ACNGT	4 cut(s)	545, 571, 1127, 1201
Bst6I	CTCTTC	1 cut(s)	480
BstAFI	CTTAAG	1 cut(s)	413
BstC8I	GCNNGC	2 cut(s)	153, 1216
BstDEI	CTNAG	3 cut(s)	137, 430, 870
BstDSI	CCRYGG	1 cut(s)	180
BstF5I	GGATG	2 cut(s)	19, 1108
BstHHI	GCGC	1 cut(s)	339
BstKTI	GATC	8 cut(s)	31, 331, 732, 793, 967, 998, 1009, 1116
BstMAI	GTCTC	2 cut(s)	272, 1137
BstMBI	GATC	8 cut(s)	28, 328, 729, 790, 964, 995, 1006, 1113
BstMWI	GCNNNNNNNGC	6 cut(s)	107, 116, 122, 320, 767, 1091
BstNI	CCWGG	2 cut(s)	468, 777
BstNSI	RCATGY	2 cut(s)	802, 1218
BstSCI	CCNGG	2 cut(s)	466, 775
BstSFI	CTRYAG	1 cut(s)	117
BstSLI	GKGCMC	3 cut(s)	40, 351, 1029
BstV1I	GCAGC	6 cut(s)	103, 131, 134, 465, 496, 878
BstX2I	RGATCY	3 cut(s)	964, 1006, 1113
BstYI	RGATCY	3 cut(s)	964, 1006, 1113
Bsu15I	ATCGAT	1 cut(s)	219
BsuI	GTATCC	1 cut(s)	1031
BsuRI	GGCC	6 cut(s)	314, 480, 740, 863, 1027, 1071
BsuTUI	ATCGAT	1 cut(s)	219
BtgI	CCRYGG	1 cut(s)	180
BtgZI	GCGATG	1 cut(s)	909
BtsCI	GGATG	2 cut(s)	19, 1108
BtsIMutI	CAGTG	6 cut(s)	251, 261, 1096, 1132, 1197, 1245
BveI	ACCTGC	1 cut(s)	599
Cac8I	GCNNGC	2 cut(s)	153, 1216
CaiI	CAGNNNCTG	1 cut(s)	1199
CfoI	GCGC	1 cut(s)	339
Cfr13I	GGNCC	6 cut(s)	312, 738, 861, 1025, 1026, 1070
ClaI	ATCGAT	1 cut(s)	219
CsiI	ACCWGGT	1 cut(s)	775
Csp6I	GTAC	2 cut(s)	442, 577
CviAII	CATG	9 cut(s)	32, 181, 463, 717, 745, 799, 922, 1211, 1215
CviQI	GTAC	2 cut(s)	442, 577
DdeI	CTNAG	3 cut(s)	137, 430, 870
DpnI	GATC	8 cut(s)	30, 330, 731, 792, 966, 997, 1008, 1115
DpnII	GATC	8 cut(s)	28, 328, 729, 790, 964, 995, 1006, 1113
DraIII	CACNNNGTG	1 cut(s)	974
EaeI	YGGCCR	1 cut(s)	478
Eam1104I	CTCTTC	1 cut(s)	480
EarI	CTCTTC	1 cut(s)	480
Ecl136II	GAGCTC	1 cut(s)	596
Eco130I	CCWWGG	2 cut(s)	180, 1073
Eco24I	GRGCYC	2 cut(s)	598, 1029
Eco31I	GGTCTC	2 cut(s)	272, 1137
Eco53kI	GAGCTC	1 cut(s)	596
Eco57I	CTGAAG	2 cut(s)	90, 96
Eco88I	CYCGRG	2 cut(s)	591, 1010
EcoICRI	GAGCTC	1 cut(s)	596
EcoO109I	RGGNCCY	3 cut(s)	1025, 1026, 1070
EcoRII	CCWGG	2 cut(s)	466, 775
EcoT14I	CCWWGG	2 cut(s)	180, 1073
EcoT22I	ATGCAT	1 cut(s)	1216
EcoT38I	GRGCYC	2 cut(s)	598, 1029
ErhI	CCWWGG	2 cut(s)	180, 1073
FaeI	CATG	9 cut(s)	35, 184, 466, 720, 748, 802, 925, 1214, 1218
FalI	AAGNNNNNCTT	2 cut(s)	954, 986
FaqI	GGGAC	1 cut(s)	600
FatI	CATG	9 cut(s)	31, 180, 462, 716, 744, 798, 921, 1210, 1214
FauI	CCCGC	1 cut(s)	1015
FauNDI	CATATG	1 cut(s)	857
FbaI	TGATCA	2 cut(s)	28, 328
Fnu4HI	GCNGC	6 cut(s)	117, 120, 123, 454, 510, 892
FokI	GGATG	2 cut(s)	26, 1115
FriOI	GRGCYC	2 cut(s)	598, 1029
Fsp4HI	GCNGC	6 cut(s)	117, 120, 123, 454, 510, 892
FspBI	CTAG	4 cut(s)	41, 344, 419, 735
GlaI	GCGC	1 cut(s)	338
GluI	GCNGC	6 cut(s)	117, 120, 123, 454, 510, 892
GsaI	CCCAGC	1 cut(s)	313
GsuI	CTGGAG	1 cut(s)	709
HaeIII	GGCC	6 cut(s)	314, 480, 740, 863, 1027, 1071
HhaI	GCGC	1 cut(s)	339
Hin1II	CATG	9 cut(s)	35, 184, 466, 720, 748, 802, 925, 1214, 1218
Hin6I	GCGC	1 cut(s)	337
HinP1I	GCGC	1 cut(s)	337
HincII	GTYRAC	2 cut(s)	295, 1063
HindII	GTYRAC	2 cut(s)	295, 1063
HinfI	GANTC	4 cut(s)	168, 199, 557, 1205
HpaI	GTTAAC	1 cut(s)	295
HphI	GGTGA	1 cut(s)	32
Hpy166II	GTNNAC	7 cut(s)	38, 295, 400, 473, 958, 1063, 1204
Hpy188I	TCNGA	7 cut(s)	140, 433, 600, 712, 995, 1000, 1184
Hpy188III	TCNNGA	6 cut(s)	326, 794, 1010, 1012, 1035, 1117
Hpy8I	GTNNAC	7 cut(s)	38, 295, 400, 473, 958, 1063, 1204
HpyAV	CCTTC	4 cut(s)	4, 890, 995, 1162
HpyCH4III	ACNGT	4 cut(s)	545, 571, 1127, 1201
HpyF10VI	GCNNNNNNNGC	6 cut(s)	107, 116, 122, 320, 767, 1091
HpyF3I	CTNAG	3 cut(s)	137, 430, 870
Hsp92II	CATG	9 cut(s)	35, 184, 466, 720, 748, 802, 925, 1214, 1218
HspAI	GCGC	1 cut(s)	337
KpnI	GGTACC	1 cut(s)	580
Ksp22I	TGATCA	2 cut(s)	28, 328
KspAI	GTTAAC	1 cut(s)	295
Kzo9I	GATC	8 cut(s)	28, 328, 729, 790, 964, 995, 1006, 1113
LguI	GCTCTTC	1 cut(s)	480
LmnI	GCTCC	2 cut(s)	880, 1153
Lsp1109I	GCAGC	6 cut(s)	103, 131, 134, 465, 496, 878
LweI	GCATC	3 cut(s)	238, 424, 484
MabI	ACCWGGT	1 cut(s)	775
MaeI	CTAG	4 cut(s)	41, 344, 419, 735
MaeIII	GTNAC	2 cut(s)	43, 1087
MalI	GATC	8 cut(s)	30, 330, 731, 792, 966, 997, 1008, 1115
MboI	GATC	8 cut(s)	28, 328, 729, 790, 964, 995, 1006, 1113
MboII	GAAGA	5 cut(s)	19, 96, 120, 497, 875
MflI	RGATCY	3 cut(s)	964, 1006, 1113
MhlI	GDGCHC	5 cut(s)	40, 351, 598, 1029, 1158
MlsI	TGGCCA	1 cut(s)	480
MluCI	AATT	4 cut(s)	318, 423, 519, 764
MluNI	TGGCCA	1 cut(s)	480
MlyI	GAGTC	3 cut(s)	162, 551, 1199
MmeI	TCCRAC	2 cut(s)	176, 1162
MnlI	CCTC	6 cut(s)	186, 812, 894, 937, 1061, 1157
Mox20I	TGGCCA	1 cut(s)	480
Mph1103I	ATGCAT	1 cut(s)	1216
MroXI	GAANNNNTTC	1 cut(s)	203
MscI	TGGCCA	1 cut(s)	480
MseI	TTAA	4 cut(s)	294, 378, 414, 1246
MslI	CAYNNNNRTG	1 cut(s)	1101
Msp20I	TGGCCA	1 cut(s)	480
MspA1I	CMGCKG	2 cut(s)	116, 245
MspCI	CTTAAG	1 cut(s)	413
MspR9I	CCNGG	2 cut(s)	468, 777
MvaI	CCWGG	2 cut(s)	468, 777
MwoI	GCNNNNNNNGC	6 cut(s)	107, 116, 122, 320, 767, 1091
NcoI	CCATGG	1 cut(s)	180
NdeI	CATATG	1 cut(s)	857
NdeII	GATC	8 cut(s)	28, 328, 729, 790, 964, 995, 1006, 1113
NlaIII	CATG	9 cut(s)	35, 184, 466, 720, 748, 802, 925, 1214, 1218
NlaIV	GGNNCC	5 cut(s)	578, 876, 1026, 1027, 1028
NmuCI	GTSAC	2 cut(s)	43, 1087
NsiI	ATGCAT	1 cut(s)	1216
NspI	RCATGY	2 cut(s)	802, 1218
PaeI	GCATGC	1 cut(s)	1218
PaeR7I	CTCGAG	1 cut(s)	1010
PciI	ACATGT	1 cut(s)	798
PciSI	GCTCTTC	1 cut(s)	480
PcsI	WCGNNNNNNNCGW	1 cut(s)	53
PdmI	GAANNNNTTC	1 cut(s)	203
PfeI	GAWTC	1 cut(s)	199
PflMI	CCANNNNNTGG	1 cut(s)	186
PkrI	GCNGC	6 cut(s)	118, 121, 124, 455, 511, 893
PleI	GAGTC	3 cut(s)	162, 551, 1199
PpsI	GAGTC	3 cut(s)	162, 551, 1199
PscI	ACATGT	1 cut(s)	798
Psp124BI	GAGCTC	1 cut(s)	598
Psp6I	CCWGG	2 cut(s)	466, 775
PspFI	CCCAGC	1 cut(s)	309
PspGI	CCWGG	2 cut(s)	466, 775
PspN4I	GGNNCC	5 cut(s)	578, 876, 1026, 1027, 1028
PspOMI	GGGCCC	1 cut(s)	1025
PspPI	GGNCC	6 cut(s)	312, 738, 861, 1025, 1026, 1070
PstI	CTGCAG	1 cut(s)	121
PstNI	CAGNNNCTG	1 cut(s)	1199
PsuI	RGATCY	3 cut(s)	964, 1006, 1113
PvuII	CAGCTG	1 cut(s)	116
RsaI	GTAC	2 cut(s)	443, 578
RsaNI	GTAC	2 cut(s)	442, 577
RseI	CAYNNNNRTG	1 cut(s)	1101
SacI	GAGCTC	1 cut(s)	598
SapI	GCTCTTC	1 cut(s)	480
SaqAI	TTAA	4 cut(s)	294, 378, 414, 1246
SatI	GCNGC	6 cut(s)	117, 120, 123, 454, 510, 892
Sau3AI	GATC	8 cut(s)	28, 328, 729, 790, 964, 995, 1006, 1113
Sau96I	GGNCC	6 cut(s)	312, 738, 861, 1025, 1026, 1070
SchI	GAGTC	3 cut(s)	162, 551, 1199
ScrFI	CCNGG	2 cut(s)	468, 777
SduI	GDGCHC	5 cut(s)	40, 351, 598, 1029, 1158
SexAI	ACCWGGT	1 cut(s)	775
SfaNI	GCATC	3 cut(s)	238, 424, 484
SfcI	CTRYAG	1 cut(s)	117
Sfr274I	CTCGAG	1 cut(s)	1010
SlaI	CTCGAG	1 cut(s)	1010
SmiMI	CAYNNNNRTG	1 cut(s)	1101
SmlI	CTYRAG	2 cut(s)	413, 1010
SmoI	CTYRAG	2 cut(s)	413, 1010
SpeI	ACTAGT	1 cut(s)	40
SphI	GCATGC	1 cut(s)	1218
Sse9I	AATT	4 cut(s)	318, 423, 519, 764
SsiI	CCGC	4 cut(s)	243, 389, 1022, 1085
SspMI	CTAG	4 cut(s)	41, 344, 419, 735
SstI	GAGCTC	1 cut(s)	598
StyD4I	CCNGG	2 cut(s)	466, 775
StyI	CCWWGG	2 cut(s)	180, 1073
TaaI	ACNGT	4 cut(s)	545, 571, 1127, 1201
TaqI	TCGA	2 cut(s)	219, 1011
TasI	AATT	4 cut(s)	318, 423, 519, 764
TfiI	GAWTC	1 cut(s)	199
Tru1I	TTAA	4 cut(s)	294, 378, 414, 1246
Tru9I	TTAA	4 cut(s)	294, 378, 414, 1246
TscAI	CASTG	6 cut(s)	258, 268, 1103, 1132, 1204, 1245
TseFI	GTSAC	2 cut(s)	43, 1087
TseI	GCWGC	6 cut(s)	116, 119, 122, 453, 509, 891
Tsp45I	GTSAC	2 cut(s)	43, 1087
TspDTI	ATGAA	4 cut(s)	617, 761, 765, 849
TspGWI	ACGGA	1 cut(s)	174
TspRI	CASTG	6 cut(s)	258, 268, 1103, 1132, 1204, 1245
Van91I	CCANNNNNTGG	1 cut(s)	186
Vha464I	CTTAAG	1 cut(s)	413
VneI	GTGCAC	1 cut(s)	36
XapI	RAATTY	2 cut(s)	423, 764
XceI	RCATGY	2 cut(s)	802, 1218
XcmI	CCANNNNNNNNNTGG	1 cut(s)	968
XhoI	CTCGAG	1 cut(s)	1010
XmnI	GAANNNNTTC	1 cut(s)	203
XspI	CTAG	4 cut(s)	41, 344, 419, 735
Zsp2I	ATGCAT	1 cut(s)	1216

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.