Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0003741

Physical Location & Seq

Genomic Coordinates Forward (+)

66187548 .. 66191430

Gene Structure 3883 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00387600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1134 bp

ATGGTGAATATGAGGGGGAAAAGTGGCGGTGATGCTGCAGTAACAACGAGGATGATTGTAATGACGGTGATGATGGTATGTTCATTCATGTCACCACAACCGATGTTAATGGGTGTTTACGCTTCACCGATAACAAGAATGATGGTGGCAGATACCAAAGAGCAACAGAGACGAAATCTGCTTGCTAATGGGCTTGGAGTGACTCCTCCCATGGGGTGGAATAGTTGGAATCACTTCAACTGCAAAATTGATGAGAAAATCATCAAAGCAACTGGGGTTAATAACAGTTCTTGTTTTTCTGGTTTGACAGCTGATGCTCTGATTTCCACTGGTCTCTCTAAACTTGGATATACCTATGTTAACATAGATGATTGTTGGGCAGAAGTAGCTCGTGATGACAAGGGTATTGATTATTTGAAGTATGACAACTGTAATACTGATGGCTCCAAACCAACTGTTAGATATCCTGTCATGACCCGAGCACTGATGAAAGCAGACCGTCCCATATTCTTTTCTCTGTGTGAATGGGGAGATTTGCATCCTGCTTTATGGGGTGCTAAAGTCGGAAACAGCTGGAGAACTACTAATGACATTTCTGATACATGGGAAAGTATGGTCTCCAGAGCAGACATGAATGAAGTTTATGCTGAATATGCCAGACCTGGTGGTTGGAATGATCCTGACATGCTTGAGGTGGGAAATGGAGGAATGACAAAGGATGAATATGTAGTCCATTTTAGCATATGGGCCATATCCAAGGCTCCCCTTCTTATTGGTTGTGATGTGAGGAATATGACAAAAGAGACTATGGAGATCATCGCAAATAAAGAGGTTATCTCTGTCAACCAAGATCCACTTGGTGTCCAAGCTAAAAAGGTTAGAATGGAAGGGGATCTTGAGGTTTGGGCAGGGCCCCTTTCAGGCTACAGAGTTGCCTTACTCCTTGTCAACCGAGGCCCGTGGCGAACATCCATTACAGCCCACTGGGATGACATTGGTATCCCCCCAAACAGTGTAGTTGAAGCAAGAGATCTTTGGGAGCACAAGACACTGAAGAAACGATTTGTTGGAAACTTGACAGCCACCATGGACTCCCATACATGCAAAATGTATGTGCTGAAGCCAATCTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0048046 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00052 ko00561 ko00600 ko00603 map00052 map00561 map00600 map00603

Pfam Domains

Protein Families

Melibiase_2 Melibiase_2_C

Protein Analysis

377

Amino Acids

42.07

Weight (kDa)

6.61

Isoelectric Point (pI)

20.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	68 - 135	5.4e-12	Alpha galactosidase A
Melibiase_2	PF16499	134 - 284	4.4e-30	Alpha galactosidase A
Melibiase_C	PF17801	296 - 373	1.8e-17	Alpha galactosidase C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003741)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08380 AT5G08380
fragaria_vesca	FvH4_2g12370 FvH4_2g12370 FvH4_2g12370 FvH4_4g37361
malus_domestica	MD13G1000400.v1.1 MD16G1012600.v1.1
prunus_persica	Prupe.1G352200_v2.0.a1 Prupe.1G352200_v2.0.a1
pyrus_communis	pycom13g00040 pycom16g01180 pycom2749g00030
rosa_chinensis	RchiOBHm_Chr4g0447241 RchiOBHm_Chr6g0272391
rosa_laevigata	RLG00000005594 RLG00000013676
rosa_multiflora	Rmu_co8492007.1_g000001 Rmu_sc0018949.1_g000001 Rmu_sc0018950.1_g000001
rosa_roxburghii	Rroxscaffold_5G00387600 Rroxscaffold_7G00196300
rosa_rugosa	Rorug04G0379000 Rorug06G0069000
rosa_samantha	Rh4AG440500 Rh4BG449500 Rh4CG466600 Rh4DG448000 Rh6AG184900 Rh6BG188200 Rh6CG186100 Rh6DG177900
rosa_wichuraiana	Rw4G037690 Rw6G015990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	216
AciI	CCGC	1 cut(s)	27
AclWI	GGATC	3 cut(s)	671, 845, 900
AcuI	CTGAAG	1 cut(s)	1073
AdeI	CACNNNGTG	1 cut(s)	860
AfiI	CCNNNNNNNGG	3 cut(s)	212, 216, 920
AgsI	TTSAA	3 cut(s)	238, 418, 1022
AjnI	CCWGG	1 cut(s)	661
AluBI	AGCT	4 cut(s)	311, 389, 573, 869
AluI	AGCT	4 cut(s)	311, 389, 573, 869
Alw21I	GWGCWC	2 cut(s)	484, 1044
Alw26I	GTCTC	4 cut(s)	163, 338, 622, 797
AlwI	GGATC	3 cut(s)	671, 845, 900
Ama87I	CYCGRG	1 cut(s)	477
AoxI	GGCC	3 cut(s)	747, 911, 955
ApaI	GGGCCC	1 cut(s)	915
ApeKI	GCWGC	1 cut(s)	35
Asp700I	GAANNNNTTC	1 cut(s)	233
AspS9I	GGNCC	4 cut(s)	747, 911, 912, 956
AsuHPI	GGTGA	5 cut(s)	16, 41, 79, 84, 117
AvaI	CYCGRG	1 cut(s)	477
BaeGI	GKGCMC	1 cut(s)	915
BaeI	ACNNNNGTAYC	2 cut(s)	982, 1015
BanII	GRGCYC	1 cut(s)	915
BauI	CACGAG	1 cut(s)	390
Bbv12I	GWGCWC	2 cut(s)	484, 1044
BbvI	GCAGC	1 cut(s)	22
BccI	CCATC	3 cut(s)	67, 136, 434
BciT130I	CCWGG	1 cut(s)	663
BciVI	GTATCC	1 cut(s)	1010
BcoDI	GTCTC	4 cut(s)	163, 338, 622, 797
BfmI	CTRYAG	2 cut(s)	36, 925
BfuI	GTATCC	1 cut(s)	1010
BglII	AGATCT	1 cut(s)	1030
BisI	GCNGC	1 cut(s)	36
BlsI	GCNGC	1 cut(s)	37
Bme1390I	CCNGG	1 cut(s)	663
BmeT110I	CYCGRG	1 cut(s)	477
BmgT120I	GGNCC	4 cut(s)	747, 911, 912, 956
BmiI	GGNNCC	4 cut(s)	445, 762, 913, 914
BmrFI	CCNGG	1 cut(s)	663
BmrI	ACTGGG	2 cut(s)	282, 994
BmsI	GCATC	3 cut(s)	22, 304, 547
BmuI	ACTGGG	2 cut(s)	282, 994
BplI	GAGNNNNNCTC	2 cut(s)	821, 853
BpmI	CTGGAG	2 cut(s)	595, 604
BpuEI	CTTGAG	2 cut(s)	710, 917
BsaBI	GATNNNNATC	1 cut(s)	537
BsaI	GGTCTC	2 cut(s)	338, 622
BsaJI	CCNNGG	5 cut(s)	210, 756, 952, 959, 1086
Bsc4I	CCNNNNNNNGG	3 cut(s)	212, 216, 920
Bse1I	ACTGG	3 cut(s)	277, 334, 989
Bse8I	GATNNNNATC	1 cut(s)	537
BseBI	CCWGG	1 cut(s)	663
BseDI	CCNNGG	5 cut(s)	210, 756, 952, 959, 1086
BseGI	GGATG	5 cut(s)	57, 538, 724, 968, 994
BseJI	GATNNNNATC	1 cut(s)	537
BseLI	CCNNNNNNNGG	3 cut(s)	212, 216, 920
BseNI	ACTGG	3 cut(s)	277, 334, 989
BseRI	GAGGAG	1 cut(s)	195
BseSI	GKGCMC	1 cut(s)	915
BseXI	GCAGC	1 cut(s)	22
BshFI	GGCC	3 cut(s)	749, 913, 957
BsiHKAI	GWGCWC	2 cut(s)	484, 1044
BsiHKCI	CYCGRG	1 cut(s)	477
BslFI	GGGAC	1 cut(s)	486
BslI	CCNNNNNNNGG	3 cut(s)	212, 216, 920
BsmAI	GTCTC	4 cut(s)	163, 338, 622, 797
BsmBI	CGTCTC	1 cut(s)	163
BsmFI	GGGAC	1 cut(s)	486
BsnI	GGCC	3 cut(s)	749, 913, 957
Bso31I	GGTCTC	2 cut(s)	338, 622
BsoBI	CYCGRG	1 cut(s)	477
Bsp120I	GGGCCC	1 cut(s)	911
Bsp1286I	GDGCHC	3 cut(s)	484, 915, 1044
Bsp143I	GATC	5 cut(s)	676, 813, 850, 892, 1030
Bsp19I	CCATGG	2 cut(s)	210, 1086
BspACI	CCGC	1 cut(s)	27
BspANI	GGCC	3 cut(s)	749, 913, 957
BspHI	TCATGA	1 cut(s)	471
BspLI	GGNNCC	4 cut(s)	445, 762, 913, 914
BspMAI	CTGCAG	1 cut(s)	40
BspPI	GGATC	3 cut(s)	671, 845, 900
BspTNI	GGTCTC	2 cut(s)	338, 622
BsrI	ACTGG	3 cut(s)	277, 334, 989
BssECI	CCNNGG	5 cut(s)	210, 756, 952, 959, 1086
BssMI	GATC	5 cut(s)	676, 813, 850, 892, 1030
BssSI	CACGAG	1 cut(s)	390
BssT1I	CCWWGG	3 cut(s)	210, 756, 1086
Bst2BI	CACGAG	1 cut(s)	390
Bst2UI	CCWGG	1 cut(s)	663
Bst4CI	ACNGT	6 cut(s)	67, 287, 431, 457, 500, 1013
BstC8I	GCNNGC	1 cut(s)	183
BstDEI	CTNAG	1 cut(s)	1131
BstDSI	CCRYGG	3 cut(s)	210, 959, 1086
BstF5I	GGATG	5 cut(s)	57, 538, 724, 968, 994
BstKTI	GATC	5 cut(s)	679, 816, 853, 895, 1033
BstMAI	GTCTC	4 cut(s)	163, 338, 622, 797
BstMBI	GATC	5 cut(s)	676, 813, 850, 892, 1030
BstMWI	GCNNNNNNNGC	2 cut(s)	386, 653
BstNI	CCWGG	1 cut(s)	663
BstNSI	RCATGY	2 cut(s)	688, 1104
BstSCI	CCNGG	1 cut(s)	661
BstSFI	CTRYAG	2 cut(s)	36, 925
BstSLI	GKGCMC	1 cut(s)	915
BstV1I	GCAGC	1 cut(s)	22
BstX2I	RGATCY	3 cut(s)	850, 892, 1030
BstYI	RGATCY	3 cut(s)	850, 892, 1030
BsuI	GTATCC	1 cut(s)	1010
BsuRI	GGCC	3 cut(s)	749, 913, 957
BtgI	CCRYGG	3 cut(s)	210, 959, 1086
BtgZI	GCGATG	1 cut(s)	802
BtsCI	GGATG	5 cut(s)	57, 538, 724, 968, 994
BtsIMutI	CAGTG	5 cut(s)	327, 482, 982, 1018, 1049
Cac8I	GCNNGC	1 cut(s)	183
CciI	TCATGA	1 cut(s)	471
Cfr13I	GGNCC	4 cut(s)	747, 911, 912, 956
CsiI	ACCWGGT	1 cut(s)	661
CviAII	CATG	8 cut(s)	88, 211, 472, 603, 631, 685, 1087, 1101
DdeI	CTNAG	1 cut(s)	1131
DpnI	GATC	5 cut(s)	678, 815, 852, 894, 1032
DpnII	GATC	5 cut(s)	676, 813, 850, 892, 1030
DraIII	CACNNNGTG	1 cut(s)	860
Eco130I	CCWWGG	3 cut(s)	210, 756, 1086
Eco24I	GRGCYC	1 cut(s)	915
Eco31I	GGTCTC	2 cut(s)	338, 622
Eco32I	GATATC	1 cut(s)	464
Eco57I	CTGAAG	1 cut(s)	1073
Eco88I	CYCGRG	1 cut(s)	477
EcoO109I	RGGNCCY	2 cut(s)	911, 912
EcoRII	CCWGG	1 cut(s)	661
EcoRV	GATATC	1 cut(s)	464
EcoT14I	CCWWGG	3 cut(s)	210, 756, 1086
EcoT38I	GRGCYC	1 cut(s)	915
ErhI	CCWWGG	3 cut(s)	210, 756, 1086
Esp3I	CGTCTC	1 cut(s)	163
FaeI	CATG	8 cut(s)	91, 214, 475, 606, 634, 688, 1090, 1104
FalI	AAGNNNNNCTT	4 cut(s)	840, 872, 879, 911
FaqI	GGGAC	1 cut(s)	486
FatI	CATG	8 cut(s)	87, 210, 471, 602, 630, 684, 1086, 1100
FauNDI	CATATG	1 cut(s)	743
Fnu4HI	GCNGC	1 cut(s)	36
FokI	GGATG	5 cut(s)	64, 525, 731, 955, 1001
FriOI	GRGCYC	1 cut(s)	915
Fsp4HI	GCNGC	1 cut(s)	36
GluI	GCNGC	1 cut(s)	36
GsuI	CTGGAG	2 cut(s)	595, 604
HaeIII	GGCC	3 cut(s)	749, 913, 957
Hin1II	CATG	8 cut(s)	91, 214, 475, 606, 634, 688, 1090, 1104
HincII	GTYRAC	3 cut(s)	361, 844, 949
HindII	GTYRAC	3 cut(s)	361, 844, 949
HinfI	GANTC	3 cut(s)	202, 229, 1091
HpaI	GTTAAC	1 cut(s)	361
HphI	GGTGA	5 cut(s)	16, 41, 79, 84, 117
Hpy166II	GTNNAC	4 cut(s)	118, 361, 844, 949
Hpy188I	TCNGA	3 cut(s)	321, 566, 598
Hpy188III	TCNNGA	5 cut(s)	392, 472, 621, 680, 896
Hpy8I	GTNNAC	4 cut(s)	118, 361, 844, 949
HpyAV	CCTTC	2 cut(s)	776, 881
HpyCH4III	ACNGT	6 cut(s)	67, 287, 431, 457, 500, 1013
HpyCH4V	TGCA	4 cut(s)	38, 243, 538, 1104
HpyF10VI	GCNNNNNNNGC	2 cut(s)	386, 653
HpyF3I	CTNAG	1 cut(s)	1131
Hsp92II	CATG	8 cut(s)	91, 214, 475, 606, 634, 688, 1090, 1104
KspAI	GTTAAC	1 cut(s)	361
Kzo9I	GATC	5 cut(s)	676, 813, 850, 892, 1030
LmnI	GCTCC	3 cut(s)	449, 766, 1039
Lsp1109I	GCAGC	1 cut(s)	22
LweI	GCATC	3 cut(s)	22, 304, 547
MabI	ACCWGGT	1 cut(s)	661
MaeIII	GTNAC	3 cut(s)	40, 90, 199
MalI	GATC	5 cut(s)	678, 815, 852, 894, 1032
MboI	GATC	5 cut(s)	676, 813, 850, 892, 1030
MboII	GAAGA	1 cut(s)	1066
MflI	RGATCY	3 cut(s)	850, 892, 1030
MhlI	GDGCHC	3 cut(s)	484, 915, 1044
MluCI	AATT	1 cut(s)	246
MlyI	GAGTC	2 cut(s)	196, 1085
MmeI	TCCRAC	4 cut(s)	206, 544, 650, 1048
MnlI	CCTC	9 cut(s)	6, 42, 216, 685, 698, 780, 823, 892, 947
MroXI	GAANNNNTTC	1 cut(s)	233
MseI	TTAA	3 cut(s)	107, 279, 360
MslI	CAYNNNNRTG	1 cut(s)	987
MspA1I	CMGCKG	2 cut(s)	311, 573
MspR9I	CCNGG	1 cut(s)	663
MvaI	CCWGG	1 cut(s)	663
MwoI	GCNNNNNNNGC	2 cut(s)	386, 653
NcoI	CCATGG	2 cut(s)	210, 1086
NdeI	CATATG	1 cut(s)	743
NdeII	GATC	5 cut(s)	676, 813, 850, 892, 1030
NlaIII	CATG	8 cut(s)	91, 214, 475, 606, 634, 688, 1090, 1104
NlaIV	GGNNCC	4 cut(s)	445, 762, 913, 914
NmuCI	GTSAC	2 cut(s)	90, 199
NspI	RCATGY	2 cut(s)	688, 1104
PagI	TCATGA	1 cut(s)	471
PdmI	GAANNNNTTC	1 cut(s)	233
PfeI	GAWTC	1 cut(s)	229
PflMI	CCANNNNNTGG	1 cut(s)	216
PkrI	GCNGC	1 cut(s)	37
PleI	GAGTC	2 cut(s)	196, 1085
PpsI	GAGTC	2 cut(s)	196, 1085
Psp6I	CCWGG	1 cut(s)	661
PspGI	CCWGG	1 cut(s)	661
PspN4I	GGNNCC	4 cut(s)	445, 762, 913, 914
PspOMI	GGGCCC	1 cut(s)	911
PspPI	GGNCC	4 cut(s)	747, 911, 912, 956
PsrI	GAACNNNNNNTAC	2 cut(s)	958, 990
PstI	CTGCAG	1 cut(s)	40
PsuI	RGATCY	3 cut(s)	850, 892, 1030
PvuII	CAGCTG	2 cut(s)	311, 573
RseI	CAYNNNNRTG	1 cut(s)	987
SaqAI	TTAA	3 cut(s)	107, 279, 360
SatI	GCNGC	1 cut(s)	36
Sau3AI	GATC	5 cut(s)	676, 813, 850, 892, 1030
Sau96I	GGNCC	4 cut(s)	747, 911, 912, 956
SchI	GAGTC	2 cut(s)	196, 1085
ScrFI	CCNGG	1 cut(s)	663
SduI	GDGCHC	3 cut(s)	484, 915, 1044
SexAI	ACCWGGT	1 cut(s)	661
SfaNI	GCATC	3 cut(s)	22, 304, 547
SfcI	CTRYAG	2 cut(s)	36, 925
SmiMI	CAYNNNNRTG	1 cut(s)	987
SmlI	CTYRAG	2 cut(s)	689, 896
SmoI	CTYRAG	2 cut(s)	689, 896
Sse9I	AATT	1 cut(s)	246
SsiI	CCGC	1 cut(s)	27
StyD4I	CCNGG	1 cut(s)	661
StyI	CCWWGG	3 cut(s)	210, 756, 1086
TaaI	ACNGT	6 cut(s)	67, 287, 431, 457, 500, 1013
TasI	AATT	1 cut(s)	246
TfiI	GAWTC	1 cut(s)	229
Tru1I	TTAA	3 cut(s)	107, 279, 360
Tru9I	TTAA	3 cut(s)	107, 279, 360
TscAI	CASTG	5 cut(s)	334, 489, 989, 1018, 1056
TseFI	GTSAC	2 cut(s)	90, 199
TseI	GCWGC	1 cut(s)	35
Tsp45I	GTSAC	2 cut(s)	90, 199
TspDTI	ATGAA	6 cut(s)	72, 76, 503, 647, 651, 735
TspRI	CASTG	5 cut(s)	334, 489, 989, 1018, 1056
Van91I	CCANNNNNTGG	1 cut(s)	216
XceI	RCATGY	2 cut(s)	688, 1104
XcmI	CCANNNNNNNNNTGG	1 cut(s)	854
XmnI	GAANNNNTTC	1 cut(s)	233

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.