Rh6DG177900

alpha-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003741
Physical Location & Seq
Reverse (-)
31272720 .. 31277062
4343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG177900.1

Sequence Viewer

Length: 1083 bp
ATGAGGAAAAAGGTCGGTGGCAGTGCTGTGCCAACATCAATGATTGTAATGGCAGTGATGCTATGCTCACTCAAGTTAATGGGTGTTTCTGCTTCGCAACTCAGACGAAATCTGCTTGCCAATGGCCTTGGAGTGACTCCTCCAATGGGGTGGAATAGTTGGAATCATTTCAACTGCAAAATTGAGGAGAAAACCATCAAAGAAACTGCTGATGCTCTGGTATCCACTGGTCTCTCTAAACTTGGATATAACTATGTTAACATAGATGATTGCTGGGCAGAAATAGCTCGCGATGTAAAGGGTAATCTAGTGGCCAAGAAATCGGCATTTCCATCAGGCATTAAAGCTCTTGCGGATTATGTCCACAGCAAGGGTCTTAAGCTAGGAATCTACTCAGATGCAGGGTACTTTACTTGCAGCAAAACTATGCCTGGTTCACTTGGTCATGAAGAACAAGATGCCAATACCTTTGCTTCTTGGGGCATCGATTATTTGAAATATGATAACTGTTATAATGATGGCTCAAACCCAACAGTTAGATACCCTGTAATGACCCGAGCTCTAATGAAAGCCAGCCGTCCCATATTCTTCTCTCTCTGTGAATGGGGAGATATGCACCCTGCTTTATGGGGTGCCAAGTTAGGAAATAGCTGGAGAACTACAAAAGACATTTCTGATACATGGGAAAGTATGGTCTCCAGAGCAGACATGAATGAATTTTATGCTGAATATGCCAGGCCTGGTGGTTGGAATGATCCCGACATGCTTGAAGTGGGAAATGGAGGAATGACAAAAGATGAATATGTAGTTCATTTTAGCATATGGGCAATCTCAAAGGCTCCCCTTCTTCTTGGTTGTGATGTAAGGAATATGACAAAAGAGACTCTGGAGATCGTTGCAAATAAAGAGGTCATCTCTGTCAACCAAGATCCACTTGGTGTCCAAGCTAAAAAAGTTAGACTGGAAGGAGATCTTGAGGTACAATTGAAATGCCAGAAATTCTTCTTATATGTAGGCCAAGTGATACCGAGTAAGAGTTGTTTCAGTAGAGCGATACAAAGTAACTTCCATTGTTTGATTTGA

Protein Analysis

360

Amino Acids

39.97

Weight (kDa)

8.22

Isoelectric Point (pI)

27.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Melibiase_2 PF16499 46 - 310 1e-75 Alpha galactosidase A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 513
AccB1I GGYRCC 1 cut(s) 632
AccII CGCG 1 cut(s) 291
AciI CCGC 1 cut(s) 353
AclWI GGATC 2 cut(s) 749, 923
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 2 cut(s) 716, 998
AdeI CACNNNGTG 1 cut(s) 938
AfaI GTAC 2 cut(s) 407, 981
AfiI CCNNNNNNNGG 2 cut(s) 146, 370
AflII CTTAAG 1 cut(s) 377
AgsI TTSAA 4 cut(s) 172, 496, 770, 988
AjnI CCWGG 3 cut(s) 430, 734, 739
AluBI AGCT 6 cut(s) 287, 347, 382, 560, 651, 947
AluI AGCT 6 cut(s) 287, 347, 382, 560, 651, 947
Alw21I GWGCWC 1 cut(s) 562
Alw26I GTCTC 3 cut(s) 236, 700, 875
AlwI GGATC 2 cut(s) 749, 923
Ama87I CYCGRG 1 cut(s) 555
AoxI GGCC 4 cut(s) 124, 312, 737, 1015
ApeKI GCWGC 1 cut(s) 417
ApoI RAATTY 2 cut(s) 716, 998
Asp700I GAANNNNTTC 2 cut(s) 167, 1001
AvaI CYCGRG 1 cut(s) 555
BalI TGGCCA 1 cut(s) 314
BanI GGYRCC 1 cut(s) 632
BanII GRGCYC 1 cut(s) 562
Bbv12I GWGCWC 1 cut(s) 562
BbvI GCAGC 1 cut(s) 429
BccI CCATC 3 cut(s) 203, 340, 512
BceAI ACGGC 1 cut(s) 561
BciT130I CCWGG 3 cut(s) 432, 736, 741
BciVI GTATCC 1 cut(s) 232
BcoDI GTCTC 3 cut(s) 236, 700, 875
BfaI CTAG 2 cut(s) 308, 383
BfrI CTTAAG 1 cut(s) 377
BfuI GTATCC 1 cut(s) 232
BglII AGATCT 1 cut(s) 970
BisI GCNGC 1 cut(s) 418
BlsI GCNGC 1 cut(s) 419
Bme1390I CCNGG 3 cut(s) 432, 736, 741
BmeT110I CYCGRG 1 cut(s) 555
BmiI GGNNCC 2 cut(s) 634, 840
BmrFI CCNGG 3 cut(s) 432, 736, 741
BmsI GCATC 5 cut(s) 48, 202, 388, 448, 492
BplI GAGNNNNNCTC 2 cut(s) 899, 931
BpmI CTGGAG 3 cut(s) 673, 682, 908
BpuEI CTTGAG 2 cut(s) 56, 995
Bsa29I ATCGAT 1 cut(s) 486
BsaI GGTCTC 2 cut(s) 236, 700
BsaJI CCNNGG 1 cut(s) 127
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 370
Bse1I ACTGG 2 cut(s) 232, 966
BseBI CCWGG 3 cut(s) 432, 736, 741
BseCI ATCGAT 1 cut(s) 486
BseDI CCNNGG 1 cut(s) 127
BseLI CCNNNNNNNGG 2 cut(s) 146, 370
BseMII CTCAG 2 cut(s) 115, 408
BseNI ACTGG 2 cut(s) 232, 966
BseRI GAGGAG 2 cut(s) 129, 200
BseXI GCAGC 1 cut(s) 429
BseYI CCCAGC 1 cut(s) 273
Bsh1236I CGCG 1 cut(s) 291
BshFI GGCC 4 cut(s) 126, 314, 739, 1017
BshNI GGYRCC 1 cut(s) 632
BshVI ATCGAT 1 cut(s) 486
BsiHKAI GWGCWC 1 cut(s) 562
BsiHKCI CYCGRG 1 cut(s) 555
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 2 cut(s) 146, 370
BsmAI GTCTC 3 cut(s) 236, 700, 875
BsmFI GGGAC 1 cut(s) 564
BsnI GGCC 4 cut(s) 126, 314, 739, 1017
Bso31I GGTCTC 2 cut(s) 236, 700
BsoBI CYCGRG 1 cut(s) 555
Bsp1286I GDGCHC 1 cut(s) 562
Bsp143I GATC 4 cut(s) 754, 891, 928, 970
Bsp68I TCGCGA 1 cut(s) 291
BspACI CCGC 1 cut(s) 353
BspANI GGCC 4 cut(s) 126, 314, 739, 1017
BspCNI CTCAG 2 cut(s) 114, 407
BspDI ATCGAT 1 cut(s) 486
BspFNI CGCG 1 cut(s) 291
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 2 cut(s) 634, 840
BspPI GGATC 2 cut(s) 749, 923
BspT107I GGYRCC 1 cut(s) 632
BspTI CTTAAG 1 cut(s) 377
BspTNI GGTCTC 2 cut(s) 236, 700
BsrI ACTGG 2 cut(s) 232, 966
BssECI CCNNGG 1 cut(s) 127
BssMI GATC 4 cut(s) 754, 891, 928, 970
BssT1I CCWWGG 1 cut(s) 127
Bst2UI CCWGG 3 cut(s) 432, 736, 741
Bst4CI ACNGT 2 cut(s) 509, 535
BstAFI CTTAAG 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 117, 289, 574
BstDEI CTNAG 2 cut(s) 101, 394
BstFNI CGCG 1 cut(s) 291
BstKTI GATC 4 cut(s) 757, 894, 931, 973
BstMAI GTCTC 3 cut(s) 236, 700, 875
BstMBI GATC 4 cut(s) 754, 891, 928, 970
BstMWI GCNNNNNNNGC 2 cut(s) 284, 731
BstNI CCWGG 3 cut(s) 432, 736, 741
BstNSI RCATGY 1 cut(s) 766
BstSCI CCNGG 3 cut(s) 430, 734, 739
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 1 cut(s) 429
BstX2I RGATCY 2 cut(s) 928, 970
BstXI CCANNNNNNTGG 1 cut(s) 150
BstYI RGATCY 2 cut(s) 928, 970
Bsu15I ATCGAT 1 cut(s) 486
BsuI GTATCC 1 cut(s) 232
BsuRI GGCC 4 cut(s) 126, 314, 739, 1017
BsuTUI ATCGAT 1 cut(s) 486
BtgZI GCGATG 1 cut(s) 306
BtsI GCAGTG 2 cut(s) 28, 60
BtsIMutI CAGTG 3 cut(s) 28, 60, 225
BtuMI TCGCGA 1 cut(s) 291
Cac8I GCNNGC 3 cut(s) 117, 289, 574
CciI TCATGA 1 cut(s) 445
ClaI ATCGAT 1 cut(s) 486
Csp6I GTAC 2 cut(s) 406, 980
CviAII CATG 4 cut(s) 446, 681, 709, 763
CviQI GTAC 2 cut(s) 406, 980
DdeI CTNAG 2 cut(s) 101, 394
DpnI GATC 4 cut(s) 756, 893, 930, 972
DpnII GATC 4 cut(s) 754, 891, 928, 970
DraIII CACNNNGTG 1 cut(s) 938
EaeI YGGCCR 1 cut(s) 312
Ecl136II GAGCTC 1 cut(s) 560
Eco130I CCWWGG 1 cut(s) 127
Eco147I AGGCCT 1 cut(s) 739
Eco24I GRGCYC 1 cut(s) 562
Eco31I GGTCTC 2 cut(s) 236, 700
Eco53kI GAGCTC 1 cut(s) 560
Eco88I CYCGRG 1 cut(s) 555
EcoICRI GAGCTC 1 cut(s) 560
EcoRII CCWGG 3 cut(s) 430, 734, 739
EcoT14I CCWWGG 1 cut(s) 127
EcoT38I GRGCYC 1 cut(s) 562
ErhI CCWWGG 1 cut(s) 127
FaeI CATG 4 cut(s) 449, 684, 712, 766
FalI AAGNNNNNCTT 4 cut(s) 918, 950, 957, 989
FaqI GGGAC 1 cut(s) 564
FatI CATG 4 cut(s) 445, 680, 708, 762
FauNDI CATATG 1 cut(s) 821
Fnu4HI GCNGC 1 cut(s) 418
FriOI GRGCYC 1 cut(s) 562
Fsp4HI GCNGC 1 cut(s) 418
FspBI CTAG 2 cut(s) 308, 383
GluI GCNGC 1 cut(s) 418
GsaI CCCAGC 1 cut(s) 277
GsuI CTGGAG 3 cut(s) 673, 682, 908
HaeIII GGCC 4 cut(s) 126, 314, 739, 1017
Hin1II CATG 4 cut(s) 449, 684, 712, 766
HincII GTYRAC 2 cut(s) 259, 922
HindII GTYRAC 2 cut(s) 259, 922
HinfI GANTC 4 cut(s) 136, 163, 387, 883
HpaI GTTAAC 1 cut(s) 259
Hpy166II GTNNAC 4 cut(s) 259, 364, 437, 922
Hpy188I TCNGA 3 cut(s) 104, 397, 676
Hpy188III TCNNGA 6 cut(s) 290, 446, 699, 758, 887, 974
Hpy8I GTNNAC 4 cut(s) 259, 364, 437, 922
HpyAV CCTTC 2 cut(s) 854, 959
HpyCH4III ACNGT 2 cut(s) 509, 535
HpyCH4V TGCA 5 cut(s) 177, 401, 417, 616, 899
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 731
HpyF3I CTNAG 2 cut(s) 101, 394
Hsp92II CATG 4 cut(s) 449, 684, 712, 766
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 4 cut(s) 754, 891, 928, 970
LmnI GCTCC 1 cut(s) 844
Lsp1109I GCAGC 1 cut(s) 429
LweI GCATC 5 cut(s) 48, 202, 388, 448, 492
MaeI CTAG 2 cut(s) 308, 383
MaeIII GTNAC 2 cut(s) 133, 1061
MalI GATC 4 cut(s) 756, 893, 930, 972
MboI GATC 4 cut(s) 754, 891, 928, 970
MboII GAAGA 4 cut(s) 461, 580, 839, 994
MfeI CAATTG 1 cut(s) 983
MflI RGATCY 2 cut(s) 928, 970
MhlI GDGCHC 1 cut(s) 562
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 4 cut(s) 180, 716, 983, 998
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 2 cut(s) 130, 877
MmeI TCCRAC 2 cut(s) 140, 728
MnlI CCTC 5 cut(s) 150, 178, 776, 901, 970
Mox20I TGGCCA 1 cut(s) 314
MroXI GAANNNNTTC 2 cut(s) 167, 1001
MscI TGGCCA 1 cut(s) 314
MseI TTAA 4 cut(s) 77, 258, 342, 378
Msp20I TGGCCA 1 cut(s) 314
MspCI CTTAAG 1 cut(s) 377
MspR9I CCNGG 3 cut(s) 432, 736, 741
MunI CAATTG 1 cut(s) 983
MvaI CCWGG 3 cut(s) 432, 736, 741
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 2 cut(s) 284, 731
NdeI CATATG 1 cut(s) 821
NdeII GATC 4 cut(s) 754, 891, 928, 970
NlaIII CATG 4 cut(s) 449, 684, 712, 766
NlaIV GGNNCC 2 cut(s) 634, 840
NmuCI GTSAC 1 cut(s) 133
NruI TCGCGA 1 cut(s) 291
NspI RCATGY 1 cut(s) 766
PagI TCATGA 1 cut(s) 445
PceI AGGCCT 1 cut(s) 739
PdmI GAANNNNTTC 2 cut(s) 167, 1001
PfeI GAWTC 2 cut(s) 163, 387
PkrI GCNGC 1 cut(s) 419
PleI GAGTC 2 cut(s) 130, 877
PpsI GAGTC 2 cut(s) 130, 877
PsiI TTATAA 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 562
Psp6I CCWGG 3 cut(s) 430, 734, 739
PspFI CCCAGC 1 cut(s) 273
PspGI CCWGG 3 cut(s) 430, 734, 739
PspN4I GGNNCC 2 cut(s) 634, 840
PsuI RGATCY 2 cut(s) 928, 970
RruI TCGCGA 1 cut(s) 291
RsaI GTAC 2 cut(s) 407, 981
RsaNI GTAC 2 cut(s) 406, 980
SacI GAGCTC 1 cut(s) 562
SaqAI TTAA 4 cut(s) 77, 258, 342, 378
SatI GCNGC 1 cut(s) 418
Sau3AI GATC 4 cut(s) 754, 891, 928, 970
SchI GAGTC 2 cut(s) 130, 877
ScrFI CCNGG 3 cut(s) 432, 736, 741
SduI GDGCHC 1 cut(s) 562
SfaNI GCATC 5 cut(s) 48, 202, 388, 448, 492
SmlI CTYRAG 3 cut(s) 71, 377, 974
SmoI CTYRAG 3 cut(s) 71, 377, 974
Sse9I AATT 4 cut(s) 180, 716, 983, 998
SseBI AGGCCT 1 cut(s) 739
SsiI CCGC 1 cut(s) 353
SspMI CTAG 2 cut(s) 308, 383
SstI GAGCTC 1 cut(s) 562
StuI AGGCCT 1 cut(s) 739
StyD4I CCNGG 3 cut(s) 430, 734, 739
StyI CCWWGG 1 cut(s) 127
TaaI ACNGT 2 cut(s) 509, 535
TaqI TCGA 1 cut(s) 486
TasI AATT 4 cut(s) 180, 716, 983, 998
TfiI GAWTC 2 cut(s) 163, 387
Tru1I TTAA 4 cut(s) 77, 258, 342, 378
Tru9I TTAA 4 cut(s) 77, 258, 342, 378
TscAI CASTG 3 cut(s) 28, 60, 232
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 1 cut(s) 417
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 6 cut(s) 462, 581, 725, 729, 800, 813
TspRI CASTG 3 cut(s) 28, 60, 232
Vha464I CTTAAG 1 cut(s) 377
XapI RAATTY 2 cut(s) 716, 998
XceI RCATGY 1 cut(s) 766
XcmI CCANNNNNNNNNTGG 1 cut(s) 932
XmnI GAANNNNTTC 2 cut(s) 167, 1001
XspI CTAG 2 cut(s) 308, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.