AT5G22010

replication factor C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005505
Physical Location & Seq
Reverse (-)
7280321 .. 7287434
7114 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G22010.2

Sequence Viewer

Length: 2871 bp
ATGTCGGATATTAGGAAGTGGTTTATGAAAGCTCATGAAAAAGGGAATGGAAGTGCTCCGAAATCGACGTCTTCCAAGGCAGGTCCTGTGAAGAATGCTGCAGAGACTGCACCAATAAAATCAGAGCAGGCTAGTGAAGACTTGGAAACTGCTGATAGAAGAAAAACGAGCAAGTATTTTGGTAAAGATAAAACAAAAGTTAAAGATGAGAAAGAGGTGGAAGCAATTCCTGCCAAGAGGAAACTTAAGACTGAGTCTGATGATTTAGTCAAGCCTCGTCCAAGAAAGGTTACAAAGGTTGTTGATGATGATGATGATGATTTTGATGTACCTATTTCAAGGAAGACTCGTGATACCACTCCCAGCAAAAAACTAAAGTCGGGGTCAGGTAGAGGAATTGCAAGTAAAACTGTTGATAACGACGATGATGATGATGGTGAAGATAAGGAGACTCCTCTAAAATCTGCCGGAAGAGGCCGTGGCGGTAGAGCTGCTCCAGGGGCCTCAACTGGTGGAAGAGGTAGAGGTGGAGGGCGAGGTGGATTTATGAATTTTGGTGAAAGGAAAGACCCACCGCATAAAGGAGAAAAGGAAGTTCCTGAAGGCACCCCTGATTGCTTAGCTGGATTGACATTTGTAATTAGCGGAACACTTGACAGTTTAGAAAGAGAAGAAGCAGAAGATCTGATTAAGCGGCATGGTGGTCGTATTACAGGCTCTGTTAGCAAGAAAACGACCTACCTTTTGTGTGATGAAGACATTGGCGGAAGGAAATCTGAAAAAGCTAAAGAGCTGGGCACTAAGTTCCTTACAGAGGATGGCCTATTTGACATAATCCGGTCCTCTAAACCTGTAAAAAAGTCTCTACCTGAAAGGTCAAACAAAGGCACTGAAAAAATCTGTGCACCACCGAAGACAAGTCCTCAGAAAGAAGAAACAAGAGGAAAACCTTTGGCAAAAAGTTCGCCCAAAAAGGTCCCCCCGGCGAAAGGAAAAAATAAAATTATCGAGACTTCCTTGCCCTGGACGGAAAAATACAGACCGAAGGTTCCAAATGAGATTGTTGGGAATCAGTCGTTGGTTACTCAACTTCACAACTGGCTGTCCCACTGGCATGATCAATTTGGAGGTACTGGAAGTAAAGGAAAGGGGAAGAAATTGAATGATGCTGGTTCCAAGAAAGCTGTACTTTTGAGTGGAACACCTGGTATTGGAAAAACAACATCAGCAAAGTTGGTTAGTCAGATGCTTGGTTTCCAGGCAGTTGAGGTTAATGCTAGTGACAGTCGTGGAAAGGCGAACTCGAATATTGCCAAAGGAATTGGTGGCAGCAATGCAAATTCTGTAAAAGAACTTGTCAATAACGAAGCCATGGCTGCCAACTTCGATAGGTCAAAGCACCCGAAGACTGTTCTAATCATGGATGAAGTAGATGGAATGTCTGCAGGCGACAGGGGAGGTGTTGCAGATCTTATTGCTAGCATTAAAATATCAAAGATTCCTATCATCTGCATTTGTAATGACCGATATAGTCAAAAACTGAAGAGTTTAGTAAACTACTGTCTTCCCCTCAATTACCGGAAGCCCACGAAACAGCAGATGGCAAAAAGGCTGATGCATATTGCTAAGGCAGAAGGCCTTGAAATTAATGAAATTGCCCTGGAAGAACTCGCTGAGAGAGTGAATGGTGACATACGATTGGCCGTAAATCAGCTCCAATATATGAGCCTGTCCATGTCAGTCATCAAATACGATGATATCAGGCAGCGCCTGCTAAGCAGTGCGAAGGATGAAGATATTTCCCCTTTCACAGCTGTTGACAAGTTGTTTGGTTACAATGGTGGGAAGCTGAGAATGGATGAACGGATTGACCTAAGCATGAGTGATCCGGATTTGGTTCCCCTACTTATACAGGAAAACTATCTCAATTATAGGCCAAGCGGTAAAGATGAGGCAAAACGAATGGATTTACTCGCTCGTGCTGCTGAATCTATTGCCGATGGAGATATTATCAACGTGCAGATTCGAAGATACAGACAATGGCAGCTCTCTCAATCTTGCTGTGTTGCATCTTCTATTCTCCCAGCTTCATTGTTGCATGGATCAAGGGAGGTACTCGAGCAGGGAGAGCGTAATTTCAATAGATTTGGTGGGTGGCTTGGCAAGAATTCTACTGCGGGAAAAAATAGACGGTTAATGGAGGATCTGCATGTTCATGTCCTTGCTTCCCGTGAATCCAGCGCTGGAAGGGAGACACTTCGTGTTGATTATCTTCCCCTTCTCTTGAGCCGCTTGACTAGCCCACTTCAGACACTGCCTAAGGATGAAGCAGTTTCAGAAGTTGTTGATTTCATGAACTCCTACTCTATAAGCCAGGAGGACTTTGATACTATTTTGGAGTTGGGAAAATTTAAGGGACGTGAAAATCCGATGGAGGGTGTTCCACCTCCAGTTAAAGCAGCTTTGACCAAAAAATATAATGAGATGAATAAAACCAGAATGGTACGTGTGGCGGACATGGTGCAGCTTCCGGGAGTGAAAAAGGCGCCAAAGAAGCGAATAGCTGCTATGCTGGAACCAACTGTTGATAGTTTGAGAGATGAAGATGGTGAGCCGTTAGCAGATAATGAAGAAGGAAATGGATCAGACGCTGAAGAAGACTCAGAAGAAGCAACCGATGGAGAAAAGCTGGAGTCCAATCTTAAAAATCTGAATGCTAGAGGGATCCAAGTGGAATTGGATTTGAAAGGAGCTGGAAGCTCAGGTTCAAGAAAGGCGGCTGGTAAAGGCAGAGGCAGAGGGAAAGCAGCTGATACGTCTGCTGAGAAGAAAGCTACAGGAAGAGGCTCTGGTGCCAAGAGAAAGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

956

Amino Acids

104.38

Weight (kDa)

9.3

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT PF00533 203 - 279 7.6e-19 BRCA1 C Terminus (BRCT) domain
PTCB-BRCT PF12738 219 - 269 2e-07 twin BRCT domain
AAA_22 PF13401 392 - 481 1.1e-06 AAA domain
AAA PF00004 396 - 508 7.7e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_RFC1 PF25361 532 - 588 7.8e-12 RFC1-like, AAA+ ATPase lid domain
RFC1 PF08519 665 - 827 5.5e-44 Replication factor RFC1 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 71
Acc36I ACCTGC 1 cut(s) 71
AccB1I GGYRCC 3 cut(s) 605, 2545, 2852
AccIII TCCGGA 1 cut(s) 1888
AclWI GGATC 6 cut(s) 1880, 2110, 2211, 2650, 2719, 2732
AcoI YGGCCR 1 cut(s) 1701
AcsI RAATTY 4 cut(s) 550, 1339, 2167, 2408
AcuI CTGAAG 4 cut(s) 621, 1562, 2291, 2673
AcyI GRCGYC 2 cut(s) 68, 2546
AdeI CACNNNGTG 1 cut(s) 2261
AfaI GTAC 5 cut(s) 330, 1132, 1188, 2115, 2505
AfeI AGCGCT 1 cut(s) 2242
AfiI CCNNNNNNNGG 6 cut(s) 581, 814, 989, 1023, 1211, 2435
AflII CTTAAG 1 cut(s) 245
AflIII ACRYGT 1 cut(s) 2506
AgsI TTSAA 6 cut(s) 339, 1162, 1643, 2140, 2746, 2769
AjiI CACGTC 1 cut(s) 2420
AjnI CCWGG 6 cut(s) 496, 1022, 1204, 1257, 1659, 2373
AjuI GAANNNNNNNTTGG 2 cut(s) 1061, 1093
Alw21I GWGCWC 2 cut(s) 58, 907
Alw26I GTCTC 5 cut(s) 98, 443, 867, 1004, 2246
Alw44I GTGCAC 1 cut(s) 903
AlwI GGATC 6 cut(s) 1880, 2110, 2211, 2650, 2719, 2732
AlwNI CAGNNNCTG 6 cut(s) 86, 107, 719, 1771, 2314, 2651
Ama87I CYCGRG 1 cut(s) 2117
Aor13HI TCCGGA 1 cut(s) 1888
Aor51HI AGCGCT 1 cut(s) 2242
AoxI GGCC 6 cut(s) 475, 501, 820, 1636, 1701, 1934
ApaLI GTGCAC 1 cut(s) 903
ApoI RAATTY 4 cut(s) 550, 1339, 2167, 2408
ArsI GACNNNNNNTTYG 6 cut(s) 53, 85, 727, 759, 1811, 1843
AseI ATTAAT 1 cut(s) 1647
Asp700I GAANNNNTTC 1 cut(s) 225
AspLEI GCGC 3 cut(s) 1770, 2243, 2548
AspS9I GGNCC 4 cut(s) 83, 501, 840, 976
AsuC2I CCSGG 2 cut(s) 983, 2532
AsuHPI GGTGA 4 cut(s) 449, 569, 1700, 2621
AsuII TTCGAA 1 cut(s) 2026
AsuNHI GCTAGC 1 cut(s) 1478
AvaI CYCGRG 1 cut(s) 2117
AvaII GGWCC 3 cut(s) 83, 840, 976
AxyI CCTNAGG 1 cut(s) 2319
BaeGI GKGCMC 2 cut(s) 800, 907
BamHI GGATCC 1 cut(s) 2724
BanI GGYRCC 3 cut(s) 605, 2545, 2852
BauI CACGAG 2 cut(s) 348, 1977
BbsI GAAGAC 8 cut(s) 63, 144, 350, 762, 920, 1412, 1556, 2664
Bbv12I GWGCWC 2 cut(s) 58, 907
BccI CCATC 8 cut(s) 428, 812, 1427, 1594, 1994, 2425, 2600, 2672
BceAI ACGGC 3 cut(s) 462, 1688, 2599
BcgI CGANNNNNNTGC 4 cut(s) 686, 720, 945, 979
BciT130I CCWGG 6 cut(s) 498, 1024, 1206, 1259, 1661, 2375
BclI TGATCA 1 cut(s) 1117
BcnI CCSGG 2 cut(s) 983, 2532
BcoDI GTCTC 5 cut(s) 98, 443, 867, 1004, 2246
BfaI CTAG 5 cut(s) 132, 1278, 1479, 2298, 2718
BfmI CTRYAG 3 cut(s) 99, 1443, 2835
BfoI RGCGCY 3 cut(s) 1771, 2244, 2549
BfrI CTTAAG 1 cut(s) 245
BfuAI ACCTGC 1 cut(s) 71
BglII AGATCT 2 cut(s) 682, 1468
BlpI GCTNAGC 2 cut(s) 619, 1775
Bme1390I CCNGG 8 cut(s) 498, 983, 1024, 1206, 1259, 1661, 2375, 2532
Bme18I GGWCC 3 cut(s) 83, 840, 976
BmeT110I CYCGRG 1 cut(s) 2117
BmgBI CACGTC 1 cut(s) 2420
BmgT120I GGNCC 4 cut(s) 83, 501, 840, 976
BmrFI CCNGG 8 cut(s) 498, 983, 1024, 1206, 1259, 1661, 2375, 2532
BmsI GCATC 4 cut(s) 1156, 1236, 1605, 2078
BmtI GCTAGC 1 cut(s) 1482
BpiI GAAGAC 8 cut(s) 63, 144, 350, 762, 920, 1412, 1556, 2664
BpmI CTGGAG 3 cut(s) 480, 2433, 2711
Bpu10I CCTNAGC 3 cut(s) 1626, 1874, 2761
Bpu1102I GCTNAGC 2 cut(s) 619, 1775
Bpu14I TTCGAA 1 cut(s) 2026
BpuEI CTTGAG 1 cut(s) 2305
BpuMI CCSGG 2 cut(s) 983, 2532
BsaAI YACGTR 1 cut(s) 2507
BsaBI GATNNNNATC 1 cut(s) 1502
BsaHI GRCGYC 2 cut(s) 68, 2546
BsaJI CCNNGG 7 cut(s) 75, 478, 497, 981, 1022, 1371, 1659
BsaWI WCCGGW 3 cut(s) 837, 1578, 1888
Bsc4I CCNNNNNNNGG 6 cut(s) 581, 814, 989, 1023, 1211, 2435
Bse1I ACTGG 5 cut(s) 514, 1103, 1115, 1138, 2450
Bse21I CCTNAGG 1 cut(s) 2319
Bse3DI GCAATG 1 cut(s) 1339
Bse8I GATNNNNATC 1 cut(s) 1502
BseAI TCCGGA 1 cut(s) 1888
BseBI CCWGG 6 cut(s) 498, 1024, 1206, 1259, 1661, 2375
BseDI CCNNGG 7 cut(s) 75, 478, 497, 981, 1022, 1371, 1659
BseGI GGATG 5 cut(s) 823, 1429, 1795, 1864, 2329
BseJI GATNNNNATC 1 cut(s) 1502
BseLI CCNNNNNNNGG 6 cut(s) 581, 814, 989, 1023, 1211, 2435
BseMI GCAATG 1 cut(s) 1339
BseMII CTCAG 7 cut(s) 243, 938, 1665, 1841, 2676, 2775, 2814
BseNI ACTGG 5 cut(s) 514, 1103, 1115, 1138, 2450
BseRI GAGGAG 1 cut(s) 444
BseSI GKGCMC 2 cut(s) 800, 907
BseYI CCCAGC 3 cut(s) 362, 793, 2083
BsgI GTGCAG 3 cut(s) 93, 2039, 2543
BshFI GGCC 6 cut(s) 477, 503, 822, 1638, 1703, 1936
BshNI GGYRCC 3 cut(s) 605, 2545, 2852
BsiHKAI GWGCWC 2 cut(s) 58, 907
BsiHKCI CYCGRG 1 cut(s) 2117
BsiSI CCGG 6 cut(s) 468, 838, 983, 1579, 1889, 2531
BslFI GGGAC 3 cut(s) 962, 1090, 2430
BslI CCNNNNNNNGG 6 cut(s) 581, 814, 989, 1023, 1211, 2435
BsmAI GTCTC 5 cut(s) 98, 443, 867, 1004, 2246
BsmFI GGGAC 3 cut(s) 962, 1090, 2430
BsmI GAATGC 2 cut(s) 100, 2719
BsnI GGCC 6 cut(s) 477, 503, 822, 1638, 1703, 1936
BsoBI CYCGRG 1 cut(s) 2117
Bsp119I TTCGAA 1 cut(s) 2026
Bsp1286I GDGCHC 3 cut(s) 58, 800, 907
Bsp13I TCCGGA 1 cut(s) 1888
Bsp143I GATC 8 cut(s) 682, 1117, 1468, 1885, 2102, 2203, 2642, 2724
Bsp1720I GCTNAGC 2 cut(s) 619, 1775
Bsp19I CCATGG 1 cut(s) 1371
BspANI GGCC 6 cut(s) 477, 503, 822, 1638, 1703, 1936
BspCNI CTCAG 7 cut(s) 244, 937, 1666, 1842, 2675, 2774, 2815
BspEI TCCGGA 1 cut(s) 1888
BspHI TCATGA 2 cut(s) 34, 2352
BspMAI CTGCAG 2 cut(s) 103, 1447
BspMI ACCTGC 1 cut(s) 71
BspOI GCTAGC 1 cut(s) 1482
BspPI GGATC 6 cut(s) 1880, 2110, 2211, 2650, 2719, 2732
BspT104I TTCGAA 1 cut(s) 2026
BspT107I GGYRCC 3 cut(s) 605, 2545, 2852
BspTI CTTAAG 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 1339
BsrI ACTGG 5 cut(s) 514, 1103, 1115, 1138, 2450
BssECI CCNNGG 7 cut(s) 75, 478, 497, 981, 1022, 1371, 1659
BssMI GATC 8 cut(s) 682, 1117, 1468, 1885, 2102, 2203, 2642, 2724
BssNI GRCGYC 2 cut(s) 68, 2546
BssSI CACGAG 2 cut(s) 348, 1977
BssT1I CCWWGG 2 cut(s) 75, 1371
Bst2BI CACGAG 2 cut(s) 348, 1977
Bst2UI CCWGG 6 cut(s) 498, 1024, 1206, 1259, 1661, 2375
Bst4CI ACNGT 7 cut(s) 412, 659, 1286, 1411, 1562, 2193, 2584
Bst6I CTCTTC 4 cut(s) 466, 511, 1538, 2836
BstACI GRCGYC 2 cut(s) 68, 2546
BstAFI CTTAAG 1 cut(s) 245
BstAPI GCANNNNNTGC 3 cut(s) 107, 230, 1771
BstBAI YACGTR 1 cut(s) 2507
BstBI TTCGAA 1 cut(s) 2026
BstC8I GCNNGC 4 cut(s) 129, 1447, 1480, 1772
BstDSI CCRYGG 2 cut(s) 478, 1371
BstENI CCTNNNNNAGG 1 cut(s) 812
BstF5I GGATG 5 cut(s) 823, 1429, 1795, 1864, 2329
BstH2I RGCGCY 3 cut(s) 1771, 2244, 2549
BstHHI GCGC 3 cut(s) 1770, 2243, 2548
BstKTI GATC 8 cut(s) 685, 1120, 1471, 1888, 2105, 2206, 2645, 2727
BstMAI GTCTC 5 cut(s) 98, 443, 867, 1004, 2246
BstMBI GATC 8 cut(s) 682, 1117, 1468, 1885, 2102, 2203, 2642, 2724
BstNI CCWGG 6 cut(s) 498, 1024, 1206, 1259, 1661, 2375
BstNSI RCATGY 1 cut(s) 2213
BstSCI CCNGG 8 cut(s) 496, 981, 1022, 1204, 1257, 1659, 2373, 2530
BstSFI CTRYAG 3 cut(s) 99, 1443, 2835
BstSLI GKGCMC 2 cut(s) 800, 907
BstV2I GAAGAC 8 cut(s) 63, 144, 350, 762, 920, 1412, 1556, 2664
BstX2I RGATCY 4 cut(s) 682, 1468, 2203, 2724
BstYI RGATCY 4 cut(s) 682, 1468, 2203, 2724
Bsu36I CCTNAGG 1 cut(s) 2319
BsuRI GGCC 6 cut(s) 477, 503, 822, 1638, 1703, 1936
BtgI CCRYGG 2 cut(s) 478, 1371
BtrI CACGTC 1 cut(s) 2420
BtsCI GGATG 5 cut(s) 823, 1429, 1795, 1864, 2329
BtsI GCAGTG 2 cut(s) 1786, 2312
BtsIMutI CAGTG 4 cut(s) 888, 1108, 1786, 2312
BveI ACCTGC 1 cut(s) 71
Cac8I GCNNGC 4 cut(s) 129, 1447, 1480, 1772
CaiI CAGNNNCTG 6 cut(s) 86, 107, 719, 1771, 2314, 2651
CciI TCATGA 2 cut(s) 34, 2352
CfoI GCGC 3 cut(s) 1770, 2243, 2548
Cfr13I GGNCC 4 cut(s) 83, 501, 840, 976
CseI GACGC 1 cut(s) 2657
CsiI ACCWGGT 1 cut(s) 1204
Csp6I GTAC 5 cut(s) 329, 1131, 1187, 2114, 2504
CviQI GTAC 5 cut(s) 329, 1131, 1187, 2114, 2504
DinI GGCGCC 1 cut(s) 2547
DpnI GATC 8 cut(s) 684, 1119, 1470, 1887, 2104, 2205, 2644, 2726
DpnII GATC 8 cut(s) 682, 1117, 1468, 1885, 2102, 2203, 2642, 2724
DraIII CACNNNGTG 1 cut(s) 2261
EaeI YGGCCR 1 cut(s) 1701
Eam1104I CTCTTC 4 cut(s) 466, 511, 1538, 2836
EarI CTCTTC 4 cut(s) 466, 511, 1538, 2836
EciI GGCGGA 2 cut(s) 780, 2528
Eco130I CCWWGG 2 cut(s) 75, 1371
Eco147I AGGCCT 1 cut(s) 1638
Eco32I GATATC 1 cut(s) 1759
Eco47I GGWCC 3 cut(s) 83, 840, 976
Eco47III AGCGCT 1 cut(s) 2242
Eco57I CTGAAG 4 cut(s) 621, 1562, 2291, 2673
Eco81I CCTNAGG 1 cut(s) 2319
Eco88I CYCGRG 1 cut(s) 2117
EcoNI CCTNNNNNAGG 1 cut(s) 812
EcoO109I RGGNCCY 3 cut(s) 83, 501, 976
EcoRI GAATTC 1 cut(s) 2167
EcoRII CCWGG 6 cut(s) 496, 1022, 1204, 1257, 1659, 2373
EcoRV GATATC 1 cut(s) 1759
EcoT14I CCWWGG 2 cut(s) 75, 1371
EcoT22I ATGCAT 1 cut(s) 1620
EgeI GGCGCC 1 cut(s) 2547
EheI GGCGCC 1 cut(s) 2547
ErhI CCWWGG 2 cut(s) 75, 1371
FalI AAGNNNNNCTT 2 cut(s) 1173, 1205
FaqI GGGAC 3 cut(s) 962, 1090, 2430
FauI CCCGC 1 cut(s) 2170
FbaI TGATCA 1 cut(s) 1117
FokI GGATG 5 cut(s) 830, 1436, 1802, 1871, 2336
FspBI CTAG 5 cut(s) 132, 1278, 1479, 2298, 2718
GlaI GCGC 3 cut(s) 1769, 2242, 2547
GsaI CCCAGC 3 cut(s) 366, 797, 2087
GsuI CTGGAG 3 cut(s) 480, 2433, 2711
HaeII RGCGCY 3 cut(s) 1771, 2244, 2549
HaeIII GGCC 6 cut(s) 477, 503, 822, 1638, 1703, 1936
HapII CCGG 6 cut(s) 468, 838, 983, 1579, 1889, 2531
HgaI GACGC 1 cut(s) 2657
HhaI GCGC 3 cut(s) 1770, 2243, 2548
Hin1I GRCGYC 2 cut(s) 68, 2546
Hin6I GCGC 3 cut(s) 1768, 2241, 2546
HinP1I GCGC 3 cut(s) 1768, 2241, 2546
HincII GTYRAC 1 cut(s) 1819
HindII GTYRAC 1 cut(s) 1819
HpaII CCGG 6 cut(s) 468, 838, 983, 1579, 1889, 2531
HphI GGTGA 4 cut(s) 449, 569, 1700, 2621
Hpy166II GTNNAC 3 cut(s) 905, 1555, 1819
Hpy188III TCNNGA 8 cut(s) 35, 350, 599, 1009, 1889, 2284, 2353, 2769
Hpy8I GTNNAC 3 cut(s) 905, 1555, 1819
Hpy99I CGWCG 2 cut(s) 70, 425
HpyAV CCTTC 8 cut(s) 596, 762, 1039, 1628, 1780, 2241, 2288, 2627
HpyCH4III ACNGT 7 cut(s) 412, 659, 1286, 1411, 1562, 2193, 2584
HpyCH4IV ACGT 5 cut(s) 68, 2016, 2419, 2506, 2816
HpySE526I ACGT 5 cut(s) 68, 2016, 2419, 2506, 2816
Hsp92I GRCGYC 2 cut(s) 68, 2546
HspAI GCGC 3 cut(s) 1768, 2241, 2546
KasI GGCGCC 1 cut(s) 2545
Kpn2I TCCGGA 1 cut(s) 1888
Ksp22I TGATCA 1 cut(s) 1117
Kzo9I GATC 8 cut(s) 682, 1117, 1468, 1885, 2102, 2203, 2642, 2724
LmnI GCTCC 4 cut(s) 61, 499, 1719, 2750
LweI GCATC 4 cut(s) 1156, 1236, 1605, 2078
MabI ACCWGGT 1 cut(s) 1204
MaeI CTAG 5 cut(s) 132, 1278, 1479, 2298, 2718
MaeII ACGT 5 cut(s) 68, 2016, 2419, 2506, 2816
MaeIII GTNAC 5 cut(s) 289, 1081, 1280, 1688, 1832
MalI GATC 8 cut(s) 684, 1119, 1470, 1887, 2104, 2205, 2644, 2726
MboI GATC 8 cut(s) 682, 1117, 1468, 1885, 2102, 2203, 2642, 2724
MflI RGATCY 4 cut(s) 682, 1468, 2203, 2724
MhlI GDGCHC 3 cut(s) 58, 800, 907
Mly113I GGCGCC 1 cut(s) 2546
MlyI GAGTC 5 cut(s) 263, 340, 445, 2654, 2702
Mph1103I ATGCAT 1 cut(s) 1620
MroI TCCGGA 1 cut(s) 1888
MroXI GAANNNNTTC 1 cut(s) 225
MslI CAYNNNNRTG 2 cut(s) 1113, 2214
MspA1I CMGCKG 2 cut(s) 1814, 2810
MspCI CTTAAG 1 cut(s) 245
MspI CCGG 6 cut(s) 468, 838, 983, 1579, 1889, 2531
MspR9I CCNGG 8 cut(s) 498, 983, 1024, 1206, 1259, 1661, 2375, 2532
Mva1269I GAATGC 2 cut(s) 100, 2719
MvaI CCWGG 6 cut(s) 498, 1024, 1206, 1259, 1661, 2375
NarI GGCGCC 1 cut(s) 2546
NciI CCSGG 2 cut(s) 983, 2532
NcoI CCATGG 1 cut(s) 1371
NdeII GATC 8 cut(s) 682, 1117, 1468, 1885, 2102, 2203, 2642, 2724
NheI GCTAGC 1 cut(s) 1478
NmuCI GTSAC 2 cut(s) 1280, 1688
NsiI ATGCAT 1 cut(s) 1620
NspI RCATGY 1 cut(s) 2213
NspV TTCGAA 1 cut(s) 2026
PaeR7I CTCGAG 1 cut(s) 2117
PagI TCATGA 2 cut(s) 34, 2352
PceI AGGCCT 1 cut(s) 1638
PctI GAATGC 2 cut(s) 100, 2719
PdmI GAANNNNTTC 1 cut(s) 225
PfeI GAWTC 5 cut(s) 1069, 1498, 1988, 2023, 2234
PflFI GACNNNGTC 1 cut(s) 253
PfoI TCCNGGA 1 cut(s) 2530
PleI GAGTC 5 cut(s) 262, 340, 445, 2654, 2701
PluTI GGCGCC 1 cut(s) 2549
PpsI GAGTC 5 cut(s) 262, 340, 445, 2654, 2701
Ppu21I YACGTR 1 cut(s) 2507
PpuMI RGGWCCY 2 cut(s) 83, 976
PshBI ATTAAT 1 cut(s) 1647
Psp5II RGGWCCY 2 cut(s) 83, 976
Psp6I CCWGG 6 cut(s) 496, 1022, 1204, 1257, 1659, 2373
PspFI CCCAGC 3 cut(s) 362, 793, 2083
PspGI CCWGG 6 cut(s) 496, 1022, 1204, 1257, 1659, 2373
PspPI GGNCC 4 cut(s) 83, 501, 840, 976
PspPPI RGGWCCY 2 cut(s) 83, 976
PspXI VCTCGAGB 1 cut(s) 2117
PstI CTGCAG 2 cut(s) 103, 1447
PstNI CAGNNNCTG 6 cut(s) 86, 107, 719, 1771, 2314, 2651
PsuI RGATCY 4 cut(s) 682, 1468, 2203, 2724
PsyI GACNNNGTC 1 cut(s) 253
PvuII CAGCTG 2 cut(s) 1814, 2810
RsaI GTAC 5 cut(s) 330, 1132, 1188, 2115, 2505
RsaNI GTAC 5 cut(s) 329, 1131, 1187, 2114, 2504
RseI CAYNNNNRTG 2 cut(s) 1113, 2214
Sau3AI GATC 8 cut(s) 682, 1117, 1468, 1885, 2102, 2203, 2642, 2724
Sau96I GGNCC 4 cut(s) 83, 501, 840, 976
SchI GAGTC 5 cut(s) 263, 340, 445, 2654, 2702
ScrFI CCNGG 8 cut(s) 498, 983, 1024, 1206, 1259, 1661, 2375, 2532
SduI GDGCHC 3 cut(s) 58, 800, 907
SexAI ACCWGGT 1 cut(s) 1204
SfaNI GCATC 4 cut(s) 1156, 1236, 1605, 2078
SfcI CTRYAG 3 cut(s) 99, 1443, 2835
SfoI GGCGCC 1 cut(s) 2547
Sfr274I CTCGAG 1 cut(s) 2117
SfuI TTCGAA 1 cut(s) 2026
SinI GGWCC 3 cut(s) 83, 840, 976
SlaI CTCGAG 1 cut(s) 2117
SmiMI CAYNNNNRTG 2 cut(s) 1113, 2214
SmlI CTYRAG 3 cut(s) 245, 2117, 2284
SmoI CTYRAG 3 cut(s) 245, 2117, 2284
SseBI AGGCCT 1 cut(s) 1638
SspDI GGCGCC 1 cut(s) 2545
SspI AATATT 1 cut(s) 1309
SspMI CTAG 5 cut(s) 132, 1278, 1479, 2298, 2718
StuI AGGCCT 1 cut(s) 1638
StyD4I CCNGG 8 cut(s) 496, 981, 1022, 1204, 1257, 1659, 2373, 2530
StyI CCWWGG 2 cut(s) 75, 1371
TaaI ACNGT 7 cut(s) 412, 659, 1286, 1411, 1562, 2193, 2584
TaiI ACGT 5 cut(s) 71, 2019, 2422, 2509, 2819
TaqI TCGA 6 cut(s) 65, 1008, 1304, 1386, 2026, 2118
TaqII GACCGA 2 cut(s) 1057, 1539
TatI WGTACW 1 cut(s) 1186
TauI GCSGC 3 cut(s) 697, 2292, 2780
TfiI GAWTC 5 cut(s) 1069, 1498, 1988, 2023, 2234
TscAI CASTG 4 cut(s) 895, 1115, 1786, 2319
TseFI GTSAC 2 cut(s) 1280, 1688
Tsp45I GTSAC 2 cut(s) 1280, 1688
TspGWI ACGGA 2 cut(s) 1043, 1879
TspRI CASTG 4 cut(s) 895, 1115, 1786, 2319
Tth111I GACNNNGTC 1 cut(s) 253
Vha464I CTTAAG 1 cut(s) 245
VneI GTGCAC 1 cut(s) 903
VpaK11BI GGWCC 3 cut(s) 83, 840, 976
VspI ATTAAT 1 cut(s) 1647
XagI CCTNNNNNAGG 1 cut(s) 812
XapI RAATTY 4 cut(s) 550, 1339, 2167, 2408
XceI RCATGY 1 cut(s) 2213
XhoI CTCGAG 1 cut(s) 2117
XmnI GAANNNNTTC 1 cut(s) 225
XspI CTAG 5 cut(s) 132, 1278, 1479, 2298, 2718
ZraI GACGTC 1 cut(s) 69
Zsp2I ATGCAT 1 cut(s) 1620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.