MD02G1244300.v1.1

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005505
Physical Location & Seq
Forward (+)
29429111 .. 29430764
1654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1244300.v1.1.491

Sequence Viewer

Length: 855 bp
ATGAAGTCCCACGACAAGGGAAACACCAAGAAACCGGCGGCGGCTCCTTCAACACCTAAGATTGAGCCTAAAGAACCAGTGCAAGGAGGTCAAGAAAATTGTGGCAGGAGGAAAACTAGCAAGTATTTTCCCACAGATAAACCAAAGGATGAAAATGGAACAACTGAAGTTCCATCCAAAAGAAAGCCTCACAAGGATCCTGATGAATGTGTTAAACCCTCACCTGCAAAAAAAGCTCACAAAGTTGTTGATGACGACGATGATGATTTTGTCTCACCTAATTCGAAGAAGAAATCAGTTGAACTTGATGCCACACCTAGTAAGAAACTGAAGAGCACGTCTGGCATGGGATTCCCACAGCAGGTTACGGCTACTGATGAAGGTGGCGATGATGATAGAAAGAATGCTGAATCACCCCTCAAGCCGGCTGGTAGGGGTCGTGGTGGAAGAGGTGCTTCTGCAGGACCAGCTGGTGGAAGAGGCAGGGGCGCTGGACGAGGCGGATTTATGAATTATGGAGAAAGGAAAGATCCCCCACACAAAGGAGAAAAGGAAGTTCCTGAAGGTGCTCCAGATTGTTTAGCTGGTTTGACTTTTGTAATTAGTGGTACACTTGACAGTTTGGAAAGAGAAGAAGCAGAGGATTTGATTAAATGTCATGGCGGTTGTATTACTGTATCTGTCAGCAAGAAAACGAACTATCTTTTATGCGATGAAGATATTGAGGGAAGGAAATCTTCTAAAGCTAAAGAACTTGGTACGGCTTTTCTTACCGAGGATGGATTGTTTGATATGATTTGGGCATCTGTCGGTGCTAAATTACCTGTACAAAGCAGCAAAGAAATCTGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

285

Amino Acids

30.24

Weight (kDa)

8.33

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT PF00533 191 - 266 3e-15 BRCA1 C Terminus (BRCT) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 232
Acc36I ACCTGC 2 cut(s) 232, 352
AccB7I CCANNNNNTGG 1 cut(s) 473
AciI CCGC 4 cut(s) 38, 41, 501, 663
AclWI GGATC 3 cut(s) 191, 204, 524
AcuI CTGAAG 3 cut(s) 186, 350, 582
AfaI GTAC 3 cut(s) 610, 760, 828
AfiI CCNNNNNNNGG 6 cut(s) 16, 83, 361, 424, 473, 542
AgsI TTSAA 2 cut(s) 51, 302
AjiI CACGTC 1 cut(s) 339
AluBI AGCT 4 cut(s) 236, 470, 584, 746
AluI AGCT 4 cut(s) 236, 470, 584, 746
Alw21I GWGCWC 2 cut(s) 338, 571
Alw26I GTCTC 1 cut(s) 277
AlwI GGATC 3 cut(s) 191, 204, 524
ApeKI GCWGC 1 cut(s) 834
AspLEI GCGC 1 cut(s) 491
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 3 cut(s) 213, 267, 405
AsuII TTCGAA 1 cut(s) 284
AvaII GGWCC 1 cut(s) 464
BamHI GGATCC 1 cut(s) 196
Bbv12I GWGCWC 2 cut(s) 338, 571
BbvI GCAGC 1 cut(s) 846
BccI CCATC 2 cut(s) 181, 773
BceAI ACGGC 2 cut(s) 384, 777
BcoDI GTCTC 1 cut(s) 277
BfaI CTAG 2 cut(s) 117, 318
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 492
BfuAI ACCTGC 2 cut(s) 232, 352
BisI GCNGC 3 cut(s) 39, 42, 835
BlsI GCNGC 3 cut(s) 40, 43, 836
Bme18I GGWCC 1 cut(s) 464
BmgBI CACGTC 1 cut(s) 339
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 2 cut(s) 45, 198
BmsI GCATC 2 cut(s) 298, 812
BpmI CTGGAG 1 cut(s) 555
Bpu14I TTCGAA 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 404
BsaJI CCNNGG 1 cut(s) 774
Bsc4I CCNNNNNNNGG 6 cut(s) 16, 83, 361, 424, 473, 542
Bse118I RCCGGY 2 cut(s) 34, 424
Bse1I ACTGG 1 cut(s) 77
BseDI CCNNGG 1 cut(s) 774
BseGI GGATG 3 cut(s) 154, 173, 784
BseLI CCNNNNNNNGG 6 cut(s) 16, 83, 361, 424, 473, 542
BseNI ACTGG 1 cut(s) 77
BseXI GCAGC 1 cut(s) 846
BsiHKAI GWGCWC 2 cut(s) 338, 571
BsiSI CCGG 2 cut(s) 35, 425
BslI CCNNNNNNNGG 6 cut(s) 16, 83, 361, 424, 473, 542
BsmAI GTCTC 1 cut(s) 277
BsmI GAATGC 1 cut(s) 409
Bsp119I TTCGAA 1 cut(s) 284
Bsp1286I GDGCHC 2 cut(s) 338, 571
Bsp1407I TGTACA 1 cut(s) 826
Bsp143I GATC 2 cut(s) 196, 529
BspACI CCGC 4 cut(s) 38, 41, 501, 663
BspLI GGNNCC 2 cut(s) 45, 198
BspMAI CTGCAG 1 cut(s) 463
BspMI ACCTGC 2 cut(s) 232, 352
BspPI GGATC 3 cut(s) 191, 204, 524
BspQI GCTCTTC 1 cut(s) 326
BspT104I TTCGAA 1 cut(s) 284
BsrFI RCCGGY 2 cut(s) 34, 424
BsrGI TGTACA 1 cut(s) 826
BsrI ACTGG 1 cut(s) 77
BssAI RCCGGY 2 cut(s) 34, 424
BssECI CCNNGG 1 cut(s) 774
BssMI GATC 2 cut(s) 196, 529
Bst4CI ACNGT 2 cut(s) 620, 676
Bst6I CTCTTC 3 cut(s) 326, 442, 472
BstAUI TGTACA 1 cut(s) 826
BstBI TTCGAA 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 1 cut(s) 57
BstF5I GGATG 3 cut(s) 154, 173, 784
BstH2I RGCGCY 1 cut(s) 492
BstHHI GCGC 1 cut(s) 491
BstKTI GATC 2 cut(s) 199, 532
BstMAI GTCTC 1 cut(s) 277
BstMBI GATC 2 cut(s) 196, 529
BstMWI GCNNNNNNNGC 3 cut(s) 233, 342, 467
BstSFI CTRYAG 1 cut(s) 459
BstV1I GCAGC 1 cut(s) 846
BstX2I RGATCY 2 cut(s) 196, 529
BstYI RGATCY 2 cut(s) 196, 529
BtgZI GCGATG 2 cut(s) 402, 726
BtrI CACGTC 1 cut(s) 339
BtsCI GGATG 3 cut(s) 154, 173, 784
BtsIMutI CAGTG 1 cut(s) 84
BveI ACCTGC 2 cut(s) 232, 352
Cac8I GCNNGC 1 cut(s) 426
CfoI GCGC 1 cut(s) 491
Cfr10I RCCGGY 2 cut(s) 34, 424
Cfr13I GGNCC 1 cut(s) 464
Csp6I GTAC 3 cut(s) 609, 759, 827
CviAII CATG 2 cut(s) 346, 659
CviQI GTAC 3 cut(s) 609, 759, 827
DdeI CTNAG 1 cut(s) 57
DpnI GATC 2 cut(s) 198, 531
DpnII GATC 2 cut(s) 196, 529
Eam1104I CTCTTC 3 cut(s) 326, 442, 472
EarI CTCTTC 3 cut(s) 326, 442, 472
EciI GGCGGA 1 cut(s) 516
Eco47I GGWCC 1 cut(s) 464
Eco57I CTGAAG 3 cut(s) 186, 350, 582
FaeI CATG 2 cut(s) 349, 662
FaiI YATR 6 cut(s) 347, 509, 516, 660, 709, 794
FalI AAGNNNNNCTT 4 cut(s) 439, 471, 721, 753
FatI CATG 2 cut(s) 345, 658
Fnu4HI GCNGC 3 cut(s) 39, 42, 835
FokI GGATG 3 cut(s) 160, 161, 791
Fsp4HI GCNGC 3 cut(s) 39, 42, 835
FspBI CTAG 2 cut(s) 117, 318
GlaI GCGC 1 cut(s) 490
GluI GCNGC 3 cut(s) 39, 42, 835
GsuI CTGGAG 1 cut(s) 555
HaeII RGCGCY 1 cut(s) 492
HapII CCGG 2 cut(s) 35, 425
HhaI GCGC 1 cut(s) 491
Hin1II CATG 2 cut(s) 349, 662
Hin6I GCGC 1 cut(s) 489
HinP1I GCGC 1 cut(s) 489
HinfI GANTC 2 cut(s) 351, 410
HpaII CCGG 2 cut(s) 35, 425
HphI GGTGA 3 cut(s) 213, 267, 405
Hpy166II GTNNAC 1 cut(s) 611
Hpy188III TCNNGA 4 cut(s) 92, 200, 560, 572
Hpy8I GTNNAC 1 cut(s) 611
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 4 cut(s) 57, 374, 557, 723
HpyCH4III ACNGT 2 cut(s) 620, 676
HpyCH4IV ACGT 1 cut(s) 338
HpyCH4V TGCA 3 cut(s) 82, 227, 461
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 342, 467
HpyF3I CTNAG 1 cut(s) 57
HpySE526I ACGT 1 cut(s) 338
Hsp92II CATG 2 cut(s) 349, 662
HspAI GCGC 1 cut(s) 489
KroI GCCGGC 1 cut(s) 424
KroNI GCCGGC 1 cut(s) 426
Kzo9I GATC 2 cut(s) 196, 529
LguI GCTCTTC 1 cut(s) 326
LmnI GCTCC 2 cut(s) 49, 574
Lsp1109I GCAGC 1 cut(s) 846
LweI GCATC 2 cut(s) 298, 812
MaeI CTAG 2 cut(s) 117, 318
MaeII ACGT 1 cut(s) 338
MaeIII GTNAC 1 cut(s) 364
MalI GATC 2 cut(s) 198, 531
MboI GATC 2 cut(s) 196, 529
MboII GAAGA 8 cut(s) 298, 301, 343, 459, 489, 644, 728, 729
MflI RGATCY 2 cut(s) 196, 529
MhlI GDGCHC 2 cut(s) 338, 571
MluCI AATT 5 cut(s) 97, 280, 511, 600, 818
MroNI GCCGGC 1 cut(s) 424
MseI TTAA 2 cut(s) 213, 651
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 2 cut(s) 35, 425
Mva1269I GAATGC 1 cut(s) 409
MwoI GCNNNNNNNGC 3 cut(s) 233, 342, 467
NaeI GCCGGC 1 cut(s) 426
NdeII GATC 2 cut(s) 196, 529
NgoMIV GCCGGC 1 cut(s) 424
NlaIII CATG 2 cut(s) 349, 662
NlaIV GGNNCC 2 cut(s) 45, 198
NspV TTCGAA 1 cut(s) 284
PaqCI CACCTGC 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 326
PctI GAATGC 1 cut(s) 409
PdiI GCCGGC 1 cut(s) 426
PfeI GAWTC 2 cut(s) 351, 410
PflMI CCANNNNNTGG 1 cut(s) 473
PkrI GCNGC 3 cut(s) 40, 43, 836
PspN4I GGNNCC 2 cut(s) 45, 198
PspPI GGNCC 1 cut(s) 464
PstI CTGCAG 1 cut(s) 463
PsuI RGATCY 2 cut(s) 196, 529
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 3 cut(s) 610, 760, 828
RsaNI GTAC 3 cut(s) 609, 759, 827
SapI GCTCTTC 1 cut(s) 326
SaqAI TTAA 2 cut(s) 213, 651
SatI GCNGC 3 cut(s) 39, 42, 835
Sau3AI GATC 2 cut(s) 196, 529
Sau96I GGNCC 1 cut(s) 464
SduI GDGCHC 2 cut(s) 338, 571
SfaNI GCATC 2 cut(s) 298, 812
SfcI CTRYAG 1 cut(s) 459
SfuI TTCGAA 1 cut(s) 284
SinI GGWCC 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 5 cut(s) 97, 280, 511, 600, 818
SsiI CCGC 4 cut(s) 38, 41, 501, 663
SspMI CTAG 2 cut(s) 117, 318
TaaI ACNGT 2 cut(s) 620, 676
TaiI ACGT 1 cut(s) 341
TaqI TCGA 1 cut(s) 284
TasI AATT 5 cut(s) 97, 280, 511, 600, 818
TatI WGTACW 1 cut(s) 826
TauI GCSGC 2 cut(s) 41, 44
TfiI GAWTC 2 cut(s) 351, 410
Tru1I TTAA 2 cut(s) 213, 651
Tru9I TTAA 2 cut(s) 213, 651
TscAI CASTG 1 cut(s) 84
TseI GCWGC 1 cut(s) 834
TspDTI ATGAA 6 cut(s) 17, 165, 219, 393, 524, 729
TspRI CASTG 1 cut(s) 84
Van91I CCANNNNNTGG 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 464
XspI CTAG 2 cut(s) 117, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.