MD02G1244400.v1.1

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005505
Physical Location & Seq
Forward (+)
29430766 .. 29445417
14652 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1244400.v1.1.491

Sequence Viewer

Length: 1917 bp
ATGTCCCGATGTGATTGCACTGGAAGCTCATTGGCATCAAGTCCATCTAGCAAACAGTTGCAGTCAGATGCCTCCCTTGCTAGGTGTAAGAAGCAAACTACCGAGCATTCTACTTTTACTTGGACAGAAAAATATAGGCCAAAGGTTCCAAACGACATTACTGGGAATCAGTCACTGGTAAAGCAGCTTCATGACTGGTTGGCACATTGGAATGAGCAATTTCTTGATACTGGAAATAAGAAAACAGGAAAAAACCCAACCAATTCTGGTGCAAAAAAGGCTGTACTTTTAAGTGGAACACCTTGTATAGGGAAAACAACGTCTGCAAAGTTAGTCAGTCAAATGCTTGGTTTCCAGGCAATTGAGGTAAATGCTAGTGACAGTGGTGGGAAAGCTGATTCAAAGATTGAAAAGGGAATTGATGGAAGCAATGCAAACTCGATTAAAGAGCTTGTCAGCAACAAGGCCCTGAGCATGGATGGATCAAAGCATCCCAAAACTGTGCTGATTATGGATGAGGTTGATGGGATGTCTGCTGGAGATCGGGGTGGAGTTGCTGATCTTATTGCTAGCATAAAGATTTCAAAAATTCATATTATCTGCATTTGTAATGATCGTTATAGTCAGAAACTGAAAAGTCTTGTGAACTACTGTTTGCTTCTCAGCTTTCGCAAACCTACCAAGCAACAGATGGCAAAGAGGTTGATGCAAGTTGCAAATGCTGAAGGCCTTCAAGTTAATGAGATTGCTCTTGAGGAACTTGCAGAAAAAGTTAATGGAGACATGCGAATGGCAGTTAACCCATTGCAATATATGAGCCTCTCAATGTCAATCATTAAATATGATGATGTACGGCAGCGCTTATTAAGCAGTTCAAAGGATGAAGATATTTCACCATTCACAGCTGTTGACAAGCTGTTTGGTTTTAACACTGGAAAGTTGCGAATGGATGAGCGAGTTGACCTGAGCATGAGTGATCCTGACCTAGTCCCTCTTCTAATCCAGGAAAATTATATCAACTATAGGCCAAGTTCAGCTGTTAAAGATGATAGTGGGATAAAACGTATGAACTTGATTGCCCGTGCCGCTGAGTCTATTGGCAATGGAGATATTTTCAATGTAGAGATTAGAAAATATCGGCAATGGCAGCTTTCCCAAAGTGCTTGCCTTTCATCCTCTATAATTCCGTATGTTTCTTCGCATCAGCTCTGGAATGCTGCATTGTTGCGGGGGCAGAGAGAGACACTTGAGCAGGGAGAAAGGAATTTTAATAGATTTGGAGGGTGGCTGGGAAAGAACTCAACATTAGGAAAAAATCTTAGGCTCTTGGAAGATTTGCATGTCCATCTTCTTGCTTCTCGTGAATCTAGTTCGGGGAGTCCTACTGATGAAGCTGTGCAGGAAGTTGTGGAGTTCATGAATACTTACTCCATTAGTCAGGATGACTTTGATATTATTGTGGAGTTATCCAAATTTCAGGGGCATCCAAATCCGCTAGATGGCATACTGCCTGCTGTAAAAGCAGCTCCGACAAAAGCATACAAAGAAGGAAGCAAAACAAGGATGATACGAGCTGCAGATTTTGTTACTATTCCTGGAATGAAAAAGGTCCCTAAGAAGAGAAGTGCTGCGCTTTTAGAACCATCTGATGATGTCATTGGTGAGAACAATGATGATACTTTGGTTCAGAGTAAAGATGAAAATTCGTCAGATACAGAGGACTTGGGTTCTGCTGTTGGTGAAAAGCTGCAAAAGGAACTGCAAAGTTTGAACACCAAAGGAGTGCAAGTACGATTCGATTTGAAGGGTGTGCCAAATTCAAGTGCAAAGAAGACACCGGCTGGCAGAGGTAGAGGTGGTTCTGCTGCTGCCAGCGTGAAAAAGGGTGGCCGAGCTGGTGCCAAGAGAAAGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

639

Amino Acids

69.81

Weight (kDa)

9.14

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 95 - 205 1.4e-09 ATPase family associated with various cellular activities (AAA)
AAA_lid_RFC1 PF25361 229 - 285 2.1e-09 RFC1-like, AAA+ ATPase lid domain
RCF1-5-like_lid PF21960 233 - 274 1.2e-06 RCF1/5-like, AAA+ ATPase lid domain
RFC1 PF08519 366 - 453 1.6e-13 Replication factor RFC1 C terminal domain
RFC1 PF08519 463 - 515 2.4e-09 Replication factor RFC1 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1898
AciI CCGC 3 cut(s) 1086, 1228, 1493
AclWI GGATC 2 cut(s) 490, 971
AcoI YGGCCR 1 cut(s) 1888
AcsI RAATTY 5 cut(s) 588, 1264, 1472, 1702, 1816
AcuI CTGAAG 1 cut(s) 744
AfaI GTAC 3 cut(s) 285, 852, 1791
AfeI AGCGCT 1 cut(s) 860
AfiI CCNNNNNNNGG 4 cut(s) 81, 308, 475, 1499
AgsI TTSAA 9 cut(s) 402, 410, 585, 734, 876, 1117, 1771, 1804, 1821
AjnI CCWGG 3 cut(s) 354, 1002, 1594
Alw26I GTCTC 2 cut(s) 774, 1235
AlwI GGATC 2 cut(s) 490, 971
AlwNI CAGNNNCTG 2 cut(s) 175, 631
Aor51HI AGCGCT 1 cut(s) 860
AoxI GGCC 5 cut(s) 137, 465, 727, 1025, 1888
ApoI RAATTY 5 cut(s) 588, 1264, 1472, 1702, 1816
ArsI GACNNNNNNTTYG 2 cut(s) 902, 934
Asp700I GAANNNNTTC 1 cut(s) 729
AspLEI GCGC 2 cut(s) 861, 1633
AspS9I GGNCC 2 cut(s) 466, 1609
AsuHPI GGTGA 3 cut(s) 885, 1673, 1751
AsuNHI GCTAGC 1 cut(s) 569
AvaII GGWCC 1 cut(s) 1609
BaeI ACNNNNGTAYC 2 cut(s) 1668, 1701
BanI GGYRCC 1 cut(s) 1898
BarI GAAGNNNNNNTAC 2 cut(s) 171, 203
BauI CACGAG 1 cut(s) 1359
BbsI GAAGAC 1 cut(s) 1838
BccI CCATC 8 cut(s) 52, 416, 473, 518, 685, 1353, 1493, 1651
BceAI ACGGC 1 cut(s) 869
BcgI CGANNNNNNTGC 1 cut(s) 31
BciT130I CCWGG 3 cut(s) 356, 1004, 1596
BcoDI GTCTC 2 cut(s) 774, 1235
BfaI CTAG 7 cut(s) 48, 81, 375, 570, 986, 1368, 1496
BfmI CTRYAG 2 cut(s) 1021, 1575
BfoI RGCGCY 1 cut(s) 862
Bme1390I CCNGG 3 cut(s) 356, 1004, 1596
Bme18I GGWCC 1 cut(s) 1609
BmgT120I GGNCC 2 cut(s) 466, 1609
BmiI GGNNCC 3 cut(s) 147, 1611, 1900
BmrFI CCNGG 3 cut(s) 356, 1004, 1596
BmrI ACTGGG 1 cut(s) 171
BmsI GCATC 6 cut(s) 44, 58, 499, 696, 1210, 1492
BmtI GCTAGC 1 cut(s) 573
BmuI ACTGGG 1 cut(s) 171
BpiI GAAGAC 1 cut(s) 1838
BpmI CTGGAG 1 cut(s) 558
Bpu10I CCTNAGC 2 cut(s) 470, 965
BpuEI CTTGAG 2 cut(s) 773, 1268
BsaXI ACNNNNNCTCC 2 cut(s) 1773, 1803
Bsc4I CCNNNNNNNGG 4 cut(s) 81, 308, 475, 1499
Bse118I RCCGGY 1 cut(s) 1837
Bse1I ACTGG 6 cut(s) 25, 166, 180, 200, 235, 937
Bse3DI GCAATG 4 cut(s) 436, 803, 1108, 1148
BseBI CCWGG 3 cut(s) 356, 1004, 1596
BseLI CCNNNNNNNGG 4 cut(s) 81, 308, 475, 1499
BseMI GCAATG 4 cut(s) 436, 803, 1108, 1148
BseMII CTCAG 4 cut(s) 461, 676, 956, 1080
BseNI ACTGG 6 cut(s) 25, 166, 180, 200, 235, 937
BseYI CCCAGC 1 cut(s) 1288
BsgI GTGCAG 1 cut(s) 1418
BshFI GGCC 5 cut(s) 139, 467, 729, 1027, 1890
BshNI GGYRCC 1 cut(s) 1898
BsiSI CCGG 1 cut(s) 1838
BslFI GGGAC 2 cut(s) 974, 1595
BslI CCNNNNNNNGG 4 cut(s) 81, 308, 475, 1499
BsmAI GTCTC 2 cut(s) 774, 1235
BsmFI GGGAC 2 cut(s) 974, 1595
BsmI GAATGC 2 cut(s) 106, 1219
BsnI GGCC 5 cut(s) 139, 467, 729, 1027, 1890
Bsp143I GATC 5 cut(s) 482, 541, 559, 613, 976
BspACI CCGC 3 cut(s) 1086, 1228, 1493
BspANI GGCC 5 cut(s) 139, 467, 729, 1027, 1890
BspCNI CTCAG 4 cut(s) 462, 675, 957, 1081
BspHI TCATGA 2 cut(s) 190, 1416
BspLI GGNNCC 3 cut(s) 147, 1611, 1900
BspMAI CTGCAG 1 cut(s) 1579
BspOI GCTAGC 1 cut(s) 573
BspPI GGATC 2 cut(s) 490, 971
BspT107I GGYRCC 1 cut(s) 1898
BsrDI GCAATG 4 cut(s) 436, 803, 1108, 1148
BsrFI RCCGGY 1 cut(s) 1837
BsrI ACTGG 6 cut(s) 25, 166, 180, 200, 235, 937
BssAI RCCGGY 1 cut(s) 1837
BssMI GATC 5 cut(s) 482, 541, 559, 613, 976
BssSI CACGAG 1 cut(s) 1359
Bst2BI CACGAG 1 cut(s) 1359
Bst2UI CCWGG 3 cut(s) 356, 1004, 1596
Bst4CI ACNGT 4 cut(s) 57, 383, 502, 653
Bst6I CTCTTC 2 cut(s) 999, 1613
BstC8I GCNNGC 5 cut(s) 571, 1165, 1512, 1843, 1873
BstDEI CTNAG 6 cut(s) 470, 662, 965, 1089, 1319, 1614
BstENI CCTNNNNNAGG 1 cut(s) 306
BstH2I RGCGCY 1 cut(s) 862
BstHHI GCGC 2 cut(s) 861, 1633
BstKTI GATC 5 cut(s) 485, 544, 562, 616, 979
BstMAI GTCTC 2 cut(s) 774, 1235
BstMBI GATC 5 cut(s) 482, 541, 559, 613, 976
BstMWI GCNNNNNNNGC 7 cut(s) 24, 77, 278, 867, 1085, 1147, 1520
BstNI CCWGG 3 cut(s) 356, 1004, 1596
BstNSI RCATGY 2 cut(s) 787, 1343
BstSCI CCNGG 3 cut(s) 354, 1002, 1594
BstSFI CTRYAG 2 cut(s) 1021, 1575
BstV2I GAAGAC 1 cut(s) 1838
BsuRI GGCC 5 cut(s) 139, 467, 729, 1027, 1890
BtsIMutI CAGTG 4 cut(s) 18, 173, 388, 930
Cac8I GCNNGC 5 cut(s) 571, 1165, 1512, 1843, 1873
CaiI CAGNNNCTG 2 cut(s) 175, 631
CciI TCATGA 2 cut(s) 190, 1416
CfoI GCGC 2 cut(s) 861, 1633
Cfr10I RCCGGY 1 cut(s) 1837
Cfr13I GGNCC 2 cut(s) 466, 1609
Csp6I GTAC 3 cut(s) 284, 851, 1790
CviAII CATG 6 cut(s) 191, 475, 784, 970, 1340, 1417
CviQI GTAC 3 cut(s) 284, 851, 1790
DdeI CTNAG 6 cut(s) 470, 662, 965, 1089, 1319, 1614
DpnI GATC 5 cut(s) 484, 543, 561, 615, 978
DpnII GATC 5 cut(s) 482, 541, 559, 613, 976
EaeI YGGCCR 1 cut(s) 1888
Eam1104I CTCTTC 2 cut(s) 999, 1613
EarI CTCTTC 2 cut(s) 999, 1613
Eco147I AGGCCT 1 cut(s) 729
Eco47I GGWCC 1 cut(s) 1609
Eco47III AGCGCT 1 cut(s) 860
Eco57I CTGAAG 1 cut(s) 744
EcoNI CCTNNNNNAGG 1 cut(s) 306
EcoO109I RGGNCCY 2 cut(s) 466, 1609
EcoRII CCWGG 3 cut(s) 354, 1002, 1594
FaeI CATG 6 cut(s) 194, 478, 787, 973, 1343, 1420
FaqI GGGAC 2 cut(s) 974, 1595
FatI CATG 6 cut(s) 190, 474, 783, 969, 1339, 1416
FauI CCCGC 1 cut(s) 1221
FspBI CTAG 7 cut(s) 48, 81, 375, 570, 986, 1368, 1496
GlaI GCGC 2 cut(s) 860, 1632
GsaI CCCAGC 1 cut(s) 1292
GsuI CTGGAG 1 cut(s) 558
HaeII RGCGCY 1 cut(s) 862
HaeIII GGCC 5 cut(s) 139, 467, 729, 1027, 1890
HapII CCGG 1 cut(s) 1838
HhaI GCGC 2 cut(s) 861, 1633
Hin1II CATG 6 cut(s) 194, 478, 787, 973, 1343, 1420
Hin6I GCGC 2 cut(s) 859, 1631
HinP1I GCGC 2 cut(s) 859, 1631
HincII GTYRAC 3 cut(s) 799, 910, 961
HindII GTYRAC 3 cut(s) 799, 910, 961
HinfI GANTC 6 cut(s) 166, 398, 1091, 1364, 1378, 1794
HpaI GTTAAC 1 cut(s) 799
HpaII CCGG 1 cut(s) 1838
HphI GGTGA 3 cut(s) 885, 1673, 1751
Hpy166II GTNNAC 4 cut(s) 646, 799, 910, 961
Hpy188I TCNGA 6 cut(s) 67, 627, 1530, 1648, 1689, 1711
Hpy188III TCNNGA 9 cut(s) 6, 191, 224, 752, 980, 1210, 1361, 1417, 1439
Hpy8I GTNNAC 4 cut(s) 646, 799, 910, 961
HpyAV CCTTC 4 cut(s) 719, 740, 1541, 1798
HpyCH4III ACNGT 4 cut(s) 57, 383, 502, 653
HpyCH4IV ACGT 2 cut(s) 320, 1063
HpyF10VI GCNNNNNNNGC 7 cut(s) 24, 77, 278, 867, 1085, 1147, 1520
HpyF3I CTNAG 6 cut(s) 470, 662, 965, 1089, 1319, 1614
HpySE526I ACGT 2 cut(s) 320, 1063
Hsp92II CATG 6 cut(s) 194, 478, 787, 973, 1343, 1420
HspAI GCGC 2 cut(s) 859, 1631
KspAI GTTAAC 1 cut(s) 799
Kzo9I GATC 5 cut(s) 482, 541, 559, 613, 976
LmnI GCTCC 1 cut(s) 1531
LweI GCATC 6 cut(s) 44, 58, 499, 696, 1210, 1492
MaeI CTAG 7 cut(s) 48, 81, 375, 570, 986, 1368, 1496
MaeII ACGT 2 cut(s) 320, 1063
MaeIII GTNAC 3 cut(s) 171, 377, 1585
MalI GATC 5 cut(s) 484, 543, 561, 615, 978
MboI GATC 5 cut(s) 482, 541, 559, 613, 976
MboII GAAGA 7 cut(s) 896, 986, 1188, 1340, 1343, 1630, 1843
MfeI CAATTG 1 cut(s) 360
MlyI GAGTC 2 cut(s) 1100, 1387
MmeI TCCRAC 1 cut(s) 1553
MroXI GAANNNNTTC 1 cut(s) 729
MslI CAYNNNNRTG 2 cut(s) 210, 788
MspA1I CMGCKG 3 cut(s) 905, 1037, 1088
MspI CCGG 1 cut(s) 1838
MspR9I CCNGG 3 cut(s) 356, 1004, 1596
MunI CAATTG 1 cut(s) 360
Mva1269I GAATGC 2 cut(s) 106, 1219
MvaI CCWGG 3 cut(s) 356, 1004, 1596
MwoI GCNNNNNNNGC 7 cut(s) 24, 77, 278, 867, 1085, 1147, 1520
NdeII GATC 5 cut(s) 482, 541, 559, 613, 976
NheI GCTAGC 1 cut(s) 569
NlaIII CATG 6 cut(s) 194, 478, 787, 973, 1343, 1420
NlaIV GGNNCC 3 cut(s) 147, 1611, 1900
NmeAIII GCCGAG 1 cut(s) 1916
NmuCI GTSAC 2 cut(s) 171, 377
NspI RCATGY 2 cut(s) 787, 1343
PagI TCATGA 2 cut(s) 190, 1416
PceI AGGCCT 1 cut(s) 729
PctI GAATGC 2 cut(s) 106, 1219
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 4 cut(s) 166, 398, 1364, 1794
PflFI GACNNNGTC 1 cut(s) 986
PfoI TCCNGGA 2 cut(s) 1002, 1594
PleI GAGTC 2 cut(s) 1099, 1386
PpsI GAGTC 2 cut(s) 1099, 1386
PpuMI RGGWCCY 1 cut(s) 1609
Psp5II RGGWCCY 1 cut(s) 1609
Psp6I CCWGG 3 cut(s) 354, 1002, 1594
PspFI CCCAGC 1 cut(s) 1288
PspGI CCWGG 3 cut(s) 354, 1002, 1594
PspN4I GGNNCC 3 cut(s) 147, 1611, 1900
PspPI GGNCC 2 cut(s) 466, 1609
PspPPI RGGWCCY 1 cut(s) 1609
PsrI GAACNNNNNNTAC 2 cut(s) 1843, 1875
PstI CTGCAG 1 cut(s) 1579
PstNI CAGNNNCTG 2 cut(s) 175, 631
PsyI GACNNNGTC 1 cut(s) 986
PvuII CAGCTG 2 cut(s) 905, 1037
RsaI GTAC 3 cut(s) 285, 852, 1791
RsaNI GTAC 3 cut(s) 284, 851, 1790
RseI CAYNNNNRTG 2 cut(s) 210, 788
Sau3AI GATC 5 cut(s) 482, 541, 559, 613, 976
Sau96I GGNCC 2 cut(s) 466, 1609
SchI GAGTC 2 cut(s) 1100, 1387
ScrFI CCNGG 3 cut(s) 356, 1004, 1596
SfaNI GCATC 6 cut(s) 44, 58, 499, 696, 1210, 1492
SfcI CTRYAG 2 cut(s) 1021, 1575
SinI GGWCC 1 cut(s) 1609
SmiMI CAYNNNNRTG 2 cut(s) 210, 788
SmlI CTYRAG 2 cut(s) 752, 1247
SmoI CTYRAG 2 cut(s) 752, 1247
SseBI AGGCCT 1 cut(s) 729
SsiI CCGC 3 cut(s) 1086, 1228, 1493
SspMI CTAG 7 cut(s) 48, 81, 375, 570, 986, 1368, 1496
StuI AGGCCT 1 cut(s) 729
StyD4I CCNGG 3 cut(s) 354, 1002, 1594
TaaI ACNGT 4 cut(s) 57, 383, 502, 653
TaiI ACGT 2 cut(s) 323, 1066
TaqI TCGA 2 cut(s) 440, 1797
TatI WGTACW 1 cut(s) 283
TauI GCSGC 1 cut(s) 1088
TfiI GAWTC 4 cut(s) 166, 398, 1364, 1794
TscAI CASTG 4 cut(s) 25, 180, 388, 937
TseFI GTSAC 2 cut(s) 171, 377
Tsp45I GTSAC 2 cut(s) 171, 377
TspGWI ACGGA 1 cut(s) 1176
TspRI CASTG 4 cut(s) 25, 180, 388, 937
Tth111I GACNNNGTC 1 cut(s) 986
VpaK11BI GGWCC 1 cut(s) 1609
XagI CCTNNNNNAGG 1 cut(s) 306
XapI RAATTY 5 cut(s) 588, 1264, 1472, 1702, 1816
XceI RCATGY 2 cut(s) 787, 1343
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 7 cut(s) 48, 81, 375, 570, 986, 1368, 1496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.