pycom02g21020

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005505
Physical Location & Seq
Forward (+)
19096092 .. 19097794
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g21020.5

Sequence Viewer

Length: 972 bp
ATGTCCCAGGCCGATATTAGGAAGTGGTTTATGAAGTCCCACGACAAGGGAAACACCAAGAAACCGGCGGCGGCTCCTTCTACACCTAAGATTGAGCCTAAAGAACCAGTGCAAGGAGGTCAAGAAAATTCTGGCAGGAGGAAAACTAGCAAGTATTTTCCCATAGATAAACCAAAGGATGAAAATGGAACAACTGAAGTTCCATCCAAAAGAAAGCCTCACAAGGATCCTGATGCATGTGTTAAACCCTCACCTGCAAAAAAAGCTCACAAAGCTGTTGATGACGACGACGATTTTGTCTTGCCTAATTCGAAGAAGAAATCAGTTGAACTTGATGCCACACCTAGTAAGAAACTGAAGAGCATATCTGGCATGGGAATCCCACGGCAGGTTACGGCTATTGATGAAGGTGGCGATGATGATAAAAAGAATGCCGAATCACCCCTCAGGCCGGCTGGTAGGGGTCGTGGTGGAAGAGGTGCTTCTGCAGGACCAGTTGGTGGAAGAGGAAGGGGCGCTGGACGAGGCGGATTTATGAATTATGGAGAAAGGAAAGATCCCCCACACAAAGGAGAAAAGGAAGTTCCTGAAGGTGCTCCAGATTGTTTAGCTGGCTTGACTTTTGTAATTAGTGGTACACTTGACAGTTTGGAAAGAGAAGAAGCAGAGGATTTGATTAAACGTCATGGCGGTCGTATTACTGGATCAACTTCAATATCCTTTGTCAGCAAGAAAACAAACTATCTTTTATGCGATGAAGATATTGAGGGAAGGAAATCCTCTAAAGCCAAAGAACTTGGTACGGCTTTTCTTACCGAGGATGGATTGTTTGATATGATTCGGGCATCTGTCGGTGCTAAATTACCTGTACAACAACCAAAGAAATCTGTGGATGATGCTGCAGCAGCATCTTTGCCCAATAAAAGCCCTAAGAAAGTAACATTGAAAAGTATGTCCCGGTTCATTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

324

Amino Acids

34.68

Weight (kDa)

9.57

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 262
AasI GACNNNNNNGTC 1 cut(s) 296
Acc36I ACCTGC 2 cut(s) 262, 379
AccB7I CCANNNNNTGG 1 cut(s) 500
AciI CCGC 4 cut(s) 68, 71, 528, 690
AclWI GGATC 4 cut(s) 221, 234, 551, 712
AcsI RAATTY 1 cut(s) 127
AcuI CTGAAG 3 cut(s) 216, 377, 609
AfaI GTAC 3 cut(s) 637, 802, 870
AfiI CCNNNNNNNGG 7 cut(s) 18, 46, 113, 388, 451, 500, 569
AgsI TTSAA 3 cut(s) 329, 714, 946
AjnI CCWGG 1 cut(s) 6
AluBI AGCT 3 cut(s) 266, 275, 611
AluI AGCT 3 cut(s) 266, 275, 611
Alw21I GWGCWC 1 cut(s) 598
AlwI GGATC 4 cut(s) 221, 234, 551, 712
AoxI GGCC 2 cut(s) 9, 449
ApeKI GCWGC 3 cut(s) 899, 902, 905
ApoI RAATTY 1 cut(s) 127
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AspLEI GCGC 1 cut(s) 518
AspS9I GGNCC 1 cut(s) 491
AsuC2I CCSGG 1 cut(s) 958
AsuHPI GGTGA 2 cut(s) 243, 432
AsuII TTCGAA 1 cut(s) 311
AvaII GGWCC 1 cut(s) 491
AxyI CCTNAGG 1 cut(s) 446
BamHI GGATCC 1 cut(s) 226
Bbv12I GWGCWC 1 cut(s) 598
BbvI GCAGC 3 cut(s) 886, 914, 917
BccI CCATC 2 cut(s) 211, 815
BceAI ACGGC 3 cut(s) 401, 411, 819
BciT130I CCWGG 1 cut(s) 8
BcnI CCSGG 1 cut(s) 958
BfaI CTAG 2 cut(s) 147, 345
BfmI CTRYAG 2 cut(s) 486, 900
BfoI RGCGCY 1 cut(s) 519
BfuAI ACCTGC 2 cut(s) 262, 379
BisI GCNGC 5 cut(s) 69, 72, 900, 903, 906
BlsI GCNGC 5 cut(s) 70, 73, 901, 904, 907
Bme1390I CCNGG 2 cut(s) 8, 958
Bme18I GGWCC 1 cut(s) 491
BmgT120I GGNCC 1 cut(s) 491
BmiI GGNNCC 2 cut(s) 75, 228
BmrFI CCNGG 2 cut(s) 8, 958
BmsI GCATC 5 cut(s) 223, 325, 854, 886, 917
BpmI CTGGAG 1 cut(s) 582
Bpu14I TTCGAA 1 cut(s) 311
BpuMI CCSGG 1 cut(s) 958
BsaJI CCNNGG 3 cut(s) 6, 383, 816
Bsc4I CCNNNNNNNGG 7 cut(s) 18, 46, 113, 388, 451, 500, 569
Bse118I RCCGGY 2 cut(s) 64, 451
Bse1I ACTGG 3 cut(s) 107, 494, 706
Bse21I CCTNAGG 1 cut(s) 446
BseBI CCWGG 1 cut(s) 8
BseDI CCNNGG 3 cut(s) 6, 383, 816
BseGI GGATG 4 cut(s) 184, 203, 826, 898
BseLI CCNNNNNNNGG 7 cut(s) 18, 46, 113, 388, 451, 500, 569
BseMII CTCAG 1 cut(s) 460
BseNI ACTGG 3 cut(s) 107, 494, 706
BseXI GCAGC 3 cut(s) 886, 914, 917
Bsh1285I CGRYCG 1 cut(s) 694
BshFI GGCC 2 cut(s) 11, 451
BsiEI CGRYCG 1 cut(s) 694
BsiHKAI GWGCWC 1 cut(s) 598
BsiSI CCGG 3 cut(s) 65, 452, 958
BslFI GGGAC 2 cut(s) 22, 940
BslI CCNNNNNNNGG 7 cut(s) 18, 46, 113, 388, 451, 500, 569
BsmFI GGGAC 2 cut(s) 22, 940
BsmI GAATGC 1 cut(s) 436
BsnI GGCC 2 cut(s) 11, 451
Bsp119I TTCGAA 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 598
Bsp1407I TGTACA 1 cut(s) 868
Bsp143I GATC 3 cut(s) 226, 556, 704
BspACI CCGC 4 cut(s) 68, 71, 528, 690
BspANI GGCC 2 cut(s) 11, 451
BspCNI CTCAG 1 cut(s) 459
BspLI GGNNCC 2 cut(s) 75, 228
BspMAI CTGCAG 2 cut(s) 490, 904
BspMI ACCTGC 2 cut(s) 262, 379
BspPI GGATC 4 cut(s) 221, 234, 551, 712
BspQI GCTCTTC 1 cut(s) 353
BspT104I TTCGAA 1 cut(s) 311
BsrFI RCCGGY 2 cut(s) 64, 451
BsrGI TGTACA 1 cut(s) 868
BsrI ACTGG 3 cut(s) 107, 494, 706
BssAI RCCGGY 2 cut(s) 64, 451
BssECI CCNNGG 3 cut(s) 6, 383, 816
BssMI GATC 3 cut(s) 226, 556, 704
Bst2UI CCWGG 1 cut(s) 8
Bst4CI ACNGT 1 cut(s) 647
Bst6I CTCTTC 3 cut(s) 353, 469, 499
BstAUI TGTACA 1 cut(s) 868
BstBI TTCGAA 1 cut(s) 311
BstC8I GCNNGC 2 cut(s) 453, 613
BstDEI CTNAG 3 cut(s) 87, 446, 930
BstDSI CCRYGG 1 cut(s) 383
BstF5I GGATG 4 cut(s) 184, 203, 826, 898
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 1 cut(s) 518
BstKTI GATC 3 cut(s) 229, 559, 707
BstMBI GATC 3 cut(s) 226, 556, 704
BstMCI CGRYCG 1 cut(s) 694
BstMWI GCNNNNNNNGC 4 cut(s) 263, 272, 369, 905
BstNI CCWGG 1 cut(s) 8
BstNSI RCATGY 1 cut(s) 240
BstSCI CCNGG 2 cut(s) 6, 956
BstSFI CTRYAG 2 cut(s) 486, 900
BstV1I GCAGC 3 cut(s) 886, 914, 917
BstX2I RGATCY 2 cut(s) 226, 556
BstYI RGATCY 2 cut(s) 226, 556
Bsu36I CCTNAGG 1 cut(s) 446
BsuRI GGCC 2 cut(s) 11, 451
BtgI CCRYGG 1 cut(s) 383
BtgZI GCGATG 2 cut(s) 429, 768
BtsCI GGATG 4 cut(s) 184, 203, 826, 898
BtsIMutI CAGTG 1 cut(s) 114
BveI ACCTGC 2 cut(s) 262, 379
Cac8I GCNNGC 2 cut(s) 453, 613
CfoI GCGC 1 cut(s) 518
Cfr10I RCCGGY 2 cut(s) 64, 451
Cfr13I GGNCC 1 cut(s) 491
Csp6I GTAC 3 cut(s) 636, 801, 869
CviAII CATG 3 cut(s) 237, 373, 686
CviQI GTAC 3 cut(s) 636, 801, 869
DdeI CTNAG 3 cut(s) 87, 446, 930
DpnI GATC 3 cut(s) 228, 558, 706
DpnII GATC 3 cut(s) 226, 556, 704
DrdI GACNNNNNNGTC 1 cut(s) 296
DseDI GACNNNNNNGTC 1 cut(s) 296
Eam1104I CTCTTC 3 cut(s) 353, 469, 499
EarI CTCTTC 3 cut(s) 353, 469, 499
EciI GGCGGA 1 cut(s) 543
Eco47I GGWCC 1 cut(s) 491
Eco57I CTGAAG 3 cut(s) 216, 377, 609
Eco81I CCTNAGG 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 6
EcoT22I ATGCAT 1 cut(s) 238
FaeI CATG 3 cut(s) 240, 376, 689
FalI AAGNNNNNCTT 2 cut(s) 466, 498
FaqI GGGAC 2 cut(s) 22, 940
FatI CATG 3 cut(s) 236, 372, 685
Fnu4HI GCNGC 5 cut(s) 69, 72, 900, 903, 906
FokI GGATG 4 cut(s) 190, 191, 833, 905
Fsp4HI GCNGC 5 cut(s) 69, 72, 900, 903, 906
FspBI CTAG 2 cut(s) 147, 345
GlaI GCGC 1 cut(s) 517
GluI GCNGC 5 cut(s) 69, 72, 900, 903, 906
GsuI CTGGAG 1 cut(s) 582
HaeII RGCGCY 1 cut(s) 519
HaeIII GGCC 2 cut(s) 11, 451
HapII CCGG 3 cut(s) 65, 452, 958
HhaI GCGC 1 cut(s) 518
Hin1II CATG 3 cut(s) 240, 376, 689
Hin6I GCGC 1 cut(s) 516
HinP1I GCGC 1 cut(s) 516
HinfI GANTC 3 cut(s) 378, 437, 838
HpaII CCGG 3 cut(s) 65, 452, 958
HphI GGTGA 2 cut(s) 243, 432
Hpy166II GTNNAC 1 cut(s) 638
Hpy188III TCNNGA 4 cut(s) 122, 230, 587, 599
Hpy8I GTNNAC 1 cut(s) 638
Hpy99I CGWCG 2 cut(s) 290, 293
HpyAV CCTTC 5 cut(s) 87, 401, 504, 584, 765
HpyCH4III ACNGT 1 cut(s) 647
HpyCH4IV ACGT 1 cut(s) 682
HpyCH4V TGCA 5 cut(s) 112, 236, 257, 488, 902
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 272, 369, 905
HpyF3I CTNAG 3 cut(s) 87, 446, 930
HpySE526I ACGT 1 cut(s) 682
Hsp92II CATG 3 cut(s) 240, 376, 689
HspAI GCGC 1 cut(s) 516
KroI GCCGGC 1 cut(s) 451
KroNI GCCGGC 1 cut(s) 453
Kzo9I GATC 3 cut(s) 226, 556, 704
LguI GCTCTTC 1 cut(s) 353
LmnI GCTCC 2 cut(s) 79, 601
Lsp1109I GCAGC 3 cut(s) 886, 914, 917
LweI GCATC 5 cut(s) 223, 325, 854, 886, 917
MaeI CTAG 2 cut(s) 147, 345
MaeII ACGT 1 cut(s) 682
MaeIII GTNAC 2 cut(s) 391, 937
MalI GATC 3 cut(s) 228, 558, 706
MboI GATC 3 cut(s) 226, 556, 704
MboII GAAGA 7 cut(s) 325, 328, 370, 486, 516, 671, 770
MflI RGATCY 2 cut(s) 226, 556
MhlI GDGCHC 1 cut(s) 598
MluCI AATT 5 cut(s) 127, 307, 538, 627, 860
Mph1103I ATGCAT 1 cut(s) 238
MroNI GCCGGC 1 cut(s) 451
MseI TTAA 3 cut(s) 243, 678, 966
MspI CCGG 3 cut(s) 65, 452, 958
MspR9I CCNGG 2 cut(s) 8, 958
Mva1269I GAATGC 1 cut(s) 436
MvaI CCWGG 1 cut(s) 8
MwoI GCNNNNNNNGC 4 cut(s) 263, 272, 369, 905
NaeI GCCGGC 1 cut(s) 453
NciI CCSGG 1 cut(s) 958
NdeII GATC 3 cut(s) 226, 556, 704
NgoMIV GCCGGC 1 cut(s) 451
NlaIII CATG 3 cut(s) 240, 376, 689
NlaIV GGNNCC 2 cut(s) 75, 228
NsiI ATGCAT 1 cut(s) 238
NspI RCATGY 1 cut(s) 240
NspV TTCGAA 1 cut(s) 311
PaqCI CACCTGC 1 cut(s) 262
PciSI GCTCTTC 1 cut(s) 353
PctI GAATGC 1 cut(s) 436
PdiI GCCGGC 1 cut(s) 453
PfeI GAWTC 3 cut(s) 378, 437, 838
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 5 cut(s) 70, 73, 901, 904, 907
Psp6I CCWGG 1 cut(s) 6
PspGI CCWGG 1 cut(s) 6
PspN4I GGNNCC 2 cut(s) 75, 228
PspPI GGNCC 1 cut(s) 491
PstI CTGCAG 2 cut(s) 490, 904
PsuI RGATCY 2 cut(s) 226, 556
RsaI GTAC 3 cut(s) 637, 802, 870
RsaNI GTAC 3 cut(s) 636, 801, 869
SapI GCTCTTC 1 cut(s) 353
SaqAI TTAA 3 cut(s) 243, 678, 966
SatI GCNGC 5 cut(s) 69, 72, 900, 903, 906
Sau3AI GATC 3 cut(s) 226, 556, 704
Sau96I GGNCC 1 cut(s) 491
ScrFI CCNGG 2 cut(s) 8, 958
SduI GDGCHC 1 cut(s) 598
SfaNI GCATC 5 cut(s) 223, 325, 854, 886, 917
SfcI CTRYAG 2 cut(s) 486, 900
SfuI TTCGAA 1 cut(s) 311
SinI GGWCC 1 cut(s) 491
Sse9I AATT 5 cut(s) 127, 307, 538, 627, 860
SsiI CCGC 4 cut(s) 68, 71, 528, 690
SspMI CTAG 2 cut(s) 147, 345
StyD4I CCNGG 2 cut(s) 6, 956
TaaI ACNGT 1 cut(s) 647
TaiI ACGT 1 cut(s) 685
TaqI TCGA 1 cut(s) 311
TasI AATT 5 cut(s) 127, 307, 538, 627, 860
TatI WGTACW 1 cut(s) 868
TauI GCSGC 2 cut(s) 71, 74
TfiI GAWTC 3 cut(s) 378, 437, 838
Tru1I TTAA 3 cut(s) 243, 678, 966
Tru9I TTAA 3 cut(s) 243, 678, 966
TscAI CASTG 1 cut(s) 114
TseI GCWGC 3 cut(s) 899, 902, 905
TspDTI ATGAA 6 cut(s) 47, 195, 420, 551, 771, 952
TspRI CASTG 1 cut(s) 114
Van91I CCANNNNNTGG 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 491
XapI RAATTY 1 cut(s) 127
XceI RCATGY 1 cut(s) 240
XspI CTAG 2 cut(s) 147, 345
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.