Rorug06G0059200

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005505
Physical Location & Seq
Forward (+)
7960960 .. 7965342
4383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0059200.1

Sequence Viewer

Length: 861 bp
ATGAATGCCAGCCGGTTGCAATGGGCATCTTCCTCGCCTGCGCTGAGCACAGGGCTCGAAACGTCGTCGTCTTGTTCGAAGCTGGGTTTTGTGAACTTTCGCGGCGGCGGCGGCGGTTGTGGAGATAGCTTGAAGACGAAGAAGGGTTTGTTTGTGATAAAGGCGATGGATGTGGAGGGTTCGAGAACCGTGAATTTGAATGGGCACGCTGGGGATTCAGATAAAAATGGGAGCTTGTTGTCTTCGATTGGAAATTCGACCAACATTCAGTGGCACCAGTGCTCCGTGAAGAAAGAAGATAGGGAAAATATGCTGAAACAGAAAGGGTGTGTTATATGGATCACTGGGCTCAGTGGATCAGGGAAGAGCACTGTGGCTTGTGCTTTGAGTGGGATCTTGTACCGCAGGGGAAAGCTGGCTTATGTTCTAGATGGGGACAATGTCAGGCATGGCTTGAATCGTGACCTTGGTTTTAAAGCAGAAGACCGTGCAGAGAACATAAGGAGGATTGGGGAGGTGGCGAAGCTGTTTGCCGATGCTGGAGTCATTTGTATTGCTAGCTTGATTTCTCCTTATAGAAGGGATCGTGATGCCTGTCGTGCATTATTGCCTGCTGGAGAGTTTATTGAGGTGTTCATGGACATTCCACTCCATGTTTGTGAGGCCAGAGACCCCAAGGGACTATATAAGCTTGCACGTGCAGGGAAGATCAAAGGTTTCACTGGGATTGATGATCCATATGAGTCGCCACTAAATTGTGAGATAGCATTAAGGCATACAGGGGGAGAATGTGCGTCCCCATGCGAGATGGCCGAAGAAGTGATTTCTTACTTAGAAGAGAAGGGGTTCCTCCAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

286

Amino Acids

30.8

Weight (kDa)

8.25

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APS_kinase PF01583 108 - 257 2.8e-70 Adenylylsulphate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 273
AccII CGCG 1 cut(s) 102
AciI CCGC 6 cut(s) 102, 105, 108, 111, 114, 403
AclWI GGATC 5 cut(s) 347, 364, 401, 591, 728
AcoI YGGCCR 1 cut(s) 810
AcsI RAATTY 2 cut(s) 193, 253
AcvI CACGTG 1 cut(s) 698
AfaI GTAC 1 cut(s) 401
AgsI TTSAA 3 cut(s) 133, 199, 457
AjnI CCWGG 1 cut(s) 852
AluBI AGCT 7 cut(s) 82, 129, 234, 415, 526, 561, 691
AluI AGCT 7 cut(s) 82, 129, 234, 415, 526, 561, 691
Alw21I GWGCWC 3 cut(s) 50, 284, 371
Alw26I GTCTC 1 cut(s) 663
AlwI GGATC 5 cut(s) 347, 364, 401, 591, 728
AoxI GGCC 2 cut(s) 663, 810
ApoI RAATTY 2 cut(s) 193, 253
Asp700I GAANNNNTTC 1 cut(s) 845
AspLEI GCGC 1 cut(s) 43
AsuII TTCGAA 1 cut(s) 77
AsuNHI GCTAGC 1 cut(s) 557
BaeGI GKGCMC 1 cut(s) 207
BanI GGYRCC 1 cut(s) 273
BanII GRGCYC 2 cut(s) 57, 351
BbrPI CACGTG 1 cut(s) 698
BbsI GAAGAC 3 cut(s) 140, 234, 489
Bbv12I GWGCWC 3 cut(s) 50, 284, 371
BccI CCATC 3 cut(s) 160, 425, 802
BcgI CGANNNNNNTGC 4 cut(s) 15, 37, 49, 71
BciT130I CCWGG 1 cut(s) 854
BcoDI GTCTC 1 cut(s) 663
BfaI CTAG 2 cut(s) 428, 558
BisI GCNGC 4 cut(s) 103, 106, 109, 112
BlpI GCTNAGC 1 cut(s) 44
BlsI GCNGC 4 cut(s) 104, 107, 110, 113
Bme1390I CCNGG 1 cut(s) 854
BmiI GGNNCC 2 cut(s) 275, 848
BmrFI CCNGG 1 cut(s) 854
BmrI ACTGGG 2 cut(s) 354, 732
BmsI GCATC 3 cut(s) 35, 526, 580
BmtI GCTAGC 1 cut(s) 561
BmuI ACTGGG 2 cut(s) 354, 732
BpiI GAAGAC 3 cut(s) 140, 234, 489
BpmI CTGGAG 3 cut(s) 561, 636, 836
Bpu1102I GCTNAGC 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 77
BsaAI YACGTR 1 cut(s) 698
BsaI GGTCTC 1 cut(s) 663
BsaJI CCNNGG 2 cut(s) 466, 675
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bse118I RCCGGY 1 cut(s) 12
Bse1I ACTGG 3 cut(s) 277, 349, 727
Bse3DI GCAATG 1 cut(s) 26
BseBI CCWGG 1 cut(s) 854
BseDI CCNNGG 2 cut(s) 466, 675
BseGI GGATG 1 cut(s) 175
BseMI GCAATG 1 cut(s) 26
BseMII CTCAG 2 cut(s) 35, 364
BseNI ACTGG 3 cut(s) 277, 349, 727
BseSI GKGCMC 1 cut(s) 207
BseYI CCCAGC 2 cut(s) 82, 209
BsgI GTGCAG 2 cut(s) 510, 720
Bsh1236I CGCG 1 cut(s) 102
BshFI GGCC 2 cut(s) 665, 812
BshNI GGYRCC 1 cut(s) 273
BsiHKAI GWGCWC 3 cut(s) 50, 284, 371
BsiSI CCGG 1 cut(s) 13
BslFI GGGAC 3 cut(s) 449, 693, 781
BsmAI GTCTC 1 cut(s) 663
BsmFI GGGAC 3 cut(s) 449, 693, 781
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 2 cut(s) 665, 812
Bso31I GGTCTC 1 cut(s) 663
Bsp119I TTCGAA 1 cut(s) 77
Bsp1286I GDGCHC 6 cut(s) 50, 57, 207, 284, 351, 371
Bsp143I GATC 6 cut(s) 339, 356, 393, 583, 708, 733
Bsp1720I GCTNAGC 1 cut(s) 44
BspACI CCGC 6 cut(s) 102, 105, 108, 111, 114, 403
BspANI GGCC 2 cut(s) 665, 812
BspCNI CTCAG 2 cut(s) 36, 363
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 2 cut(s) 275, 848
BspOI GCTAGC 1 cut(s) 561
BspPI GGATC 5 cut(s) 347, 364, 401, 591, 728
BspQI GCTCTTC 1 cut(s) 359
BspT104I TTCGAA 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 273
BspTNI GGTCTC 1 cut(s) 663
BsrDI GCAATG 1 cut(s) 26
BsrFI RCCGGY 1 cut(s) 12
BsrI ACTGG 3 cut(s) 277, 349, 727
BssAI RCCGGY 1 cut(s) 12
BssECI CCNNGG 2 cut(s) 466, 675
BssMI GATC 6 cut(s) 339, 356, 393, 583, 708, 733
BssT1I CCWWGG 2 cut(s) 466, 675
Bst2UI CCWGG 1 cut(s) 854
Bst4CI ACNGT 3 cut(s) 190, 373, 488
Bst6I CTCTTC 2 cut(s) 359, 831
BstBAI YACGTR 1 cut(s) 698
BstBI TTCGAA 1 cut(s) 77
BstC8I GCNNGC 7 cut(s) 10, 39, 207, 417, 559, 612, 693
BstDEI CTNAG 3 cut(s) 44, 350, 832
BstF5I GGATG 1 cut(s) 175
BstFNI CGCG 1 cut(s) 102
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 6 cut(s) 342, 359, 396, 586, 711, 736
BstMAI GTCTC 1 cut(s) 663
BstMBI GATC 6 cut(s) 339, 356, 393, 583, 708, 733
BstMWI GCNNNNNNNGC 3 cut(s) 108, 111, 599
BstNI CCWGG 1 cut(s) 854
BstSCI CCNGG 1 cut(s) 852
BstSLI GKGCMC 1 cut(s) 207
BstUI CGCG 1 cut(s) 102
BstV2I GAAGAC 3 cut(s) 140, 234, 489
BstX2I RGATCY 1 cut(s) 393
BstYI RGATCY 1 cut(s) 393
BsuRI GGCC 2 cut(s) 665, 812
BtgZI GCGATG 1 cut(s) 179
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 6 cut(s) 275, 284, 342, 358, 369, 720
Cac8I GCNNGC 7 cut(s) 10, 39, 207, 417, 559, 612, 693
CfoI GCGC 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 12
CseI GACGC 1 cut(s) 783
Csp6I GTAC 1 cut(s) 400
CviAII CATG 4 cut(s) 449, 637, 653, 801
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 3 cut(s) 44, 350, 832
DpnI GATC 6 cut(s) 341, 358, 395, 585, 710, 735
DpnII GATC 6 cut(s) 339, 356, 393, 583, 708, 733
DraI TTTAAA 1 cut(s) 475
EaeI YGGCCR 1 cut(s) 810
Eam1104I CTCTTC 2 cut(s) 359, 831
EarI CTCTTC 2 cut(s) 359, 831
Eco130I CCWWGG 2 cut(s) 466, 675
Eco24I GRGCYC 2 cut(s) 57, 351
Eco31I GGTCTC 1 cut(s) 663
Eco72I CACGTG 1 cut(s) 698
EcoRII CCWGG 1 cut(s) 852
EcoT14I CCWWGG 2 cut(s) 466, 675
EcoT38I GRGCYC 2 cut(s) 57, 351
ErhI CCWWGG 2 cut(s) 466, 675
FaeI CATG 4 cut(s) 452, 640, 656, 804
FaqI GGGAC 3 cut(s) 449, 693, 781
FatI CATG 4 cut(s) 448, 636, 652, 800
FauNDI CATATG 1 cut(s) 739
Fnu4HI GCNGC 4 cut(s) 103, 106, 109, 112
FokI GGATG 1 cut(s) 182
FriOI GRGCYC 2 cut(s) 57, 351
Fsp4HI GCNGC 4 cut(s) 103, 106, 109, 112
FspBI CTAG 2 cut(s) 428, 558
GlaI GCGC 1 cut(s) 42
GluI GCNGC 4 cut(s) 103, 106, 109, 112
GsaI CCCAGC 2 cut(s) 86, 213
GsuI CTGGAG 3 cut(s) 561, 636, 836
HaeIII GGCC 2 cut(s) 665, 812
HapII CCGG 1 cut(s) 13
HgaI GACGC 1 cut(s) 783
HhaI GCGC 1 cut(s) 43
Hin1II CATG 4 cut(s) 452, 640, 656, 804
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 4 cut(s) 215, 457, 543, 743
HpaII CCGG 1 cut(s) 13
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 4 cut(s) 183, 428, 461, 587
Hpy8I GTNNAC 1 cut(s) 94
Hpy99I CGWCG 2 cut(s) 67, 70
HpyAV CCTTC 3 cut(s) 136, 573, 835
HpyCH4III ACNGT 3 cut(s) 190, 373, 488
HpyCH4IV ACGT 2 cut(s) 62, 697
HpyCH4V TGCA 5 cut(s) 19, 491, 602, 695, 701
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 111, 599
HpyF3I CTNAG 3 cut(s) 44, 350, 832
HpySE526I ACGT 2 cut(s) 62, 697
Hsp92II CATG 4 cut(s) 452, 640, 656, 804
HspAI GCGC 1 cut(s) 41
Kzo9I GATC 6 cut(s) 339, 356, 393, 583, 708, 733
LguI GCTCTTC 1 cut(s) 359
LmnI GCTCC 2 cut(s) 231, 287
LweI GCATC 3 cut(s) 35, 526, 580
MaeI CTAG 2 cut(s) 428, 558
MaeII ACGT 2 cut(s) 62, 697
MaeIII GTNAC 1 cut(s) 461
MalI GATC 6 cut(s) 341, 358, 395, 585, 710, 735
MboI GATC 6 cut(s) 339, 356, 393, 583, 708, 733
MflI RGATCY 1 cut(s) 393
MhlI GDGCHC 6 cut(s) 50, 57, 207, 284, 351, 371
MluCI AATT 3 cut(s) 193, 253, 754
MlyI GAGTC 2 cut(s) 552, 752
MnlI CCTC 7 cut(s) 43, 169, 498, 508, 622, 655, 860
MroXI GAANNNNTTC 1 cut(s) 845
MseI TTAA 3 cut(s) 474, 770, 859
MslI CAYNNNNRTG 1 cut(s) 657
MspI CCGG 1 cut(s) 13
MspR9I CCNGG 1 cut(s) 854
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 1 cut(s) 854
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 3 cut(s) 108, 111, 599
NdeI CATATG 1 cut(s) 739
NdeII GATC 6 cut(s) 339, 356, 393, 583, 708, 733
NheI GCTAGC 1 cut(s) 557
NlaIII CATG 4 cut(s) 452, 640, 656, 804
NlaIV GGNNCC 2 cut(s) 275, 848
NmuCI GTSAC 1 cut(s) 461
NspV TTCGAA 1 cut(s) 77
PciSI GCTCTTC 1 cut(s) 359
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PctI GAATGC 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 845
PfeI GAWTC 2 cut(s) 215, 457
PflFI GACNNNGTC 1 cut(s) 440
PkrI GCNGC 4 cut(s) 104, 107, 110, 113
PleI GAGTC 2 cut(s) 551, 751
PmaCI CACGTG 1 cut(s) 698
PmlI CACGTG 1 cut(s) 698
PpsI GAGTC 2 cut(s) 551, 751
Ppu21I YACGTR 1 cut(s) 698
Psp6I CCWGG 1 cut(s) 852
PspCI CACGTG 1 cut(s) 698
PspFI CCCAGC 2 cut(s) 82, 209
PspGI CCWGG 1 cut(s) 852
PspN4I GGNNCC 2 cut(s) 275, 848
PsuI RGATCY 1 cut(s) 393
PsyI GACNNNGTC 1 cut(s) 440
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
RseI CAYNNNNRTG 1 cut(s) 657
SapI GCTCTTC 1 cut(s) 359
SaqAI TTAA 3 cut(s) 474, 770, 859
SatI GCNGC 4 cut(s) 103, 106, 109, 112
Sau3AI GATC 6 cut(s) 339, 356, 393, 583, 708, 733
SchI GAGTC 2 cut(s) 552, 752
ScrFI CCNGG 1 cut(s) 854
SduI GDGCHC 6 cut(s) 50, 57, 207, 284, 351, 371
SfaNI GCATC 3 cut(s) 35, 526, 580
SfuI TTCGAA 1 cut(s) 77
SmiMI CAYNNNNRTG 1 cut(s) 657
Sse9I AATT 3 cut(s) 193, 253, 754
SsiI CCGC 6 cut(s) 102, 105, 108, 111, 114, 403
SspMI CTAG 2 cut(s) 428, 558
StyD4I CCNGG 1 cut(s) 852
StyI CCWWGG 2 cut(s) 466, 675
TaaI ACNGT 3 cut(s) 190, 373, 488
TaiI ACGT 2 cut(s) 65, 700
TaqI TCGA 5 cut(s) 57, 77, 182, 245, 257
TasI AATT 3 cut(s) 193, 253, 754
TauI GCSGC 4 cut(s) 105, 108, 111, 114
TfiI GAWTC 2 cut(s) 215, 457
Tru1I TTAA 3 cut(s) 474, 770, 859
Tru9I TTAA 3 cut(s) 474, 770, 859
TscAI CASTG 6 cut(s) 275, 284, 349, 358, 376, 727
TseFI GTSAC 1 cut(s) 461
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 2 cut(s) 17, 625
TspGWI ACGGA 1 cut(s) 274
TspRI CASTG 6 cut(s) 275, 284, 349, 358, 376, 727
Tth111I GACNNNGTC 1 cut(s) 440
XapI RAATTY 2 cut(s) 193, 253
XbaI TCTAGA 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 845
XspI CTAG 2 cut(s) 428, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.