Rw5G031940

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005505
Physical Location & Seq
Forward (+)
53481848 .. 53494896
13049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G031940.1

Sequence Viewer

Length: 2916 bp
ATGTCGCAGGCAGATATCAGAAAGTGGTTTATGAAATCGCATGACAAGGCCAATGCCTCCAAACCGGCTCCGGCTCCGGCTGCTTCTGCTTTGCCGAAACAGTCTACAGACCCTCAACAACAACAGAATGTGCATGGAGGTGAAGAAAATTCAGGCAGAAGGAAAACCAGCAAGTATTTTCCTCCAAATAAACCAAAGCCGAATGAGGAAAAGGAAACCGTTGAAGTTCCAGCAAAGCGAAAGACTCGACCTGATCCTGTTGAATCTAGCAGACCCTCACCTGCAAAAAAAGTTCAGAAAGTTGCTGATGATGACGACGACGACGACGATTTTGTTTTGCCTCAATCAAAAAAGAAAGAAGCCACGCCTAGCAAGAAACTGAAGAATGCATCTGGCATGGGAATTCCACAGAACCTGACGTCTGCAGATGAAAGTGATGATGATGAAGCCAAGGCTCCTCGCAAATCTGGTGGTAGAGGTCGTGGTGGAAGAGGTAGTTCATCGGCCACACCAGCTGCTGGAAGAGGCAGAGGCGCTGGACGGGGTGGGTTTATGAATTTTGGCGAAAGGAAAGATCCTCCACACAAAGGAGAAAAGGACGTTCCTGAAGGTGATCCAAACTGTCTAGCTGGTTTAACTTTCGTAATAAGTGGTACACTTGATAGTTTGGAAAGAGAGGAGGCAGAAGATTTGATAAAACGACATGGTGGTCGTATCACTGGATCTGTCAGCAAGAAAACGAGCTATCTTTTATGTGATGAAGATATTGAGGGCAGGAAATCCTCAAAAGCCAAAGAACTTGGGACGGCTTTCCTTACTGAGGATGGATTGTTTGATAAGATTCGGGCATCAATTCGTGCTAAAGCACCTGTGAAAGAAGAAAAGAAACCGGTGGATAGTGCACGAGAATCATCTTTGCCTAATAAAAGCCCTAAGAAAGTAGCATTAAAGAGTTCACCAGATTGCACTAGCAGCTCCTTGGCATGTAGGGTATCTCACAAACAGTCGCCATCAGATGCTTCCCTTGTTAGGCGCAAAGAGCAAACTACTGAGCATTCTGCTTTGACATGGACAGAAAAATATAGACCAAAGGTTCCAAATGACATTATTGGTAACCAGTCACTGGTAAAGCAGCTTCATGATTGGTTGGTACATTGGCATGAGCAATTCCGTGATACTGGAGTTAAGAAAAAAGGCAATAAACCAAACAATACAGGTGCTAAAAAAGCTGTACTATTAAGTGGAACACCCGGAATAGGGAAAACAACGTCAGCAAAGTTGGTCAGTCAGATGCTCGGTTTCCAGGCAATTGAGGTAAATGCAAGTGACAGTCGTGGGAAAGCTGATTCCAAGATTGTCAAGGGAATCGGTGGAAGCAATGCAAACTCTGTTAAAGAGCTCGTCAGCAACGAGGCCCTAAGCATGGATAGATCTAAGCATCCCAAAACTGTGCTGATTATGGATGAGGTTGATGGGATGTCTGCTGGTGATCGGGGTGGAGTTGCTGATCTTATTGCTAGCATAAAGAGTTCTAAAATTCCCATTATCTGCATTTGTAATGATCGTTACAGTCAGAAACTGAAAAGTCTTGTGAACTACTGTTTGCTTCTCAGCTTCCGCAAACCCACTAAACAACAGATGGCAAAGAGGTTGAAGCAAGTTGCAAATGCTGAAGGACTTCAAGTGAATGAGATTGCTCTTGAGGAACTTGCAGAAAGAGTTAACGGAGACATGCGAATGGCACTTAATCAGTTGCACTATATGAGTCTCTCAATGTCTGTCATAAAATATGAAGATATAAGGCAACGCCTACTAAGCAGTGCAAAGGATGAGGATATTTCACCATTCACAGCTGTTGACAAGCTGTTTGGTTTCAATGCTGGAAAGTTGCGGATGGATGAGCGGTTTGACCTGAGCATGAGTGATCCAGATCTAGTCCCTCTTCTTATTCAGGAAAATTATATTAACTACAGGCCAAGTGCGGCTGTTAAGGATGATAGTGGGGTTGAACGTATGAACTTGATCGCCCGTGCCGCTGAGTCTATTGGAAATGGGGATATTATCAATGTACAAATTAGAAAATATCGCCAATGGCAACTTTCCCAAAGTTGTTCTCTGTCATCCAGCATAATTCCAGCTGCTTTGTTGCGTGGGCAAAGGGAGACACTTGAGCAGGGAGAAAGAAATTTTAATAGATTTGGAGGTTGGCTAGGAAAGAACTCAACATTAGGCAAAAATCTGAGGCTCTTAGAAGATTTGCATTTCCATCTTCTTGCATCTCGTGAATCTAGTTCAGGGAGGGAGACCCTGCGAGTTGAATACCTTCCCCTACTTCTGAAACGATTGTCTGTGCCGCTGCGTAAATTGTCGAAGGATGAAGCTGTTCAGGAAGTTGTGGAGTTCATGAATACTTACTCCATTAGTCAAGATGACTATGATACTATTGTAGAGTTATCAAAATTTCGGGGGCATCCAAATCCGCTGGATGGTGTTCAGCCAGCTGTAAAAGCAGCTCTTACAAAAGCATACAAAGAAGGAAGCAAATCAAGGATGGTACGAGTTGCAGAGTTTGTCACTTTCCCTGGAATGAAAAAGGCTCCTAAGAAGAGGATCGCAGCTATTTTAGAACCATCTGACGATGTAACTGGGGAGAACAATAATGATACTTTGGTTCTAAGTGAAGAAGAAAATTCATCTGATACAGAGGACTTGGAAAGCTCTGCTGTTGGGGAGAAGCTGCAACAGGAACTTCAAAGTTTGAATAAAAAAGGAGTGCAAGTTCAATTGGATTTGAAGGGTGCAACAAATCCAAGTGCCAAGAGGACACCAGCTGGAAGAGGTAAAGGTGCTTCTTCTGGTGCCTCTGAGAAAAAAGGTGGCCGAGGTTCAGGGTCTGGTGCCAAAAGAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

971

Amino Acids

106.2

Weight (kDa)

9.44

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT PF00533 206 - 279 5.5e-17 BRCA1 C Terminus (BRCT) domain
PTCB-BRCT PF12738 221 - 269 4.7e-06 twin BRCT domain
AAA_22 PF13401 408 - 498 4.9e-06 AAA domain
AAA PF00004 411 - 524 3.4e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_RFC1 PF25361 545 - 600 4.3e-08 RFC1-like, AAA+ ATPase lid domain
RCF1-5-like_lid PF21960 549 - 590 7e-07 RCF1/5-like, AAA+ ATPase lid domain
RFC1 PF08519 682 - 844 9e-41 Replication factor RFC1 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 289
AasI GACNNNNNNGTC 1 cut(s) 708
AatII GACGTC 1 cut(s) 422
Acc36I ACCTGC 1 cut(s) 289
AccB1I GGYRCC 2 cut(s) 2858, 2897
AccB7I CCANNNNNTGG 2 cut(s) 518, 1123
AccBSI CCGCTC 1 cut(s) 1903
AccI GTMKAC 1 cut(s) 104
AciI CCGC 7 cut(s) 1618, 1891, 1903, 1982, 2034, 2354, 2480
AclWI GGATC 6 cut(s) 248, 569, 608, 730, 1919, 2618
AcoI YGGCCR 2 cut(s) 504, 2878
AcsI RAATTY 7 cut(s) 148, 402, 556, 1536, 2185, 2459, 2689
AcuI CTGAAG 3 cut(s) 401, 627, 1692
AcyI GRCGYC 1 cut(s) 419
AfaI GTAC 5 cut(s) 655, 1152, 1233, 2070, 2556
AfiI CCNNNNNNNGG 9 cut(s) 518, 587, 820, 1029, 1123, 1256, 1257, 1423, 2486
AgeI ACCGGT 1 cut(s) 889
AjnI CCWGG 2 cut(s) 1302, 2581
AloI GAACNNNNNNTCC 2 cut(s) 2763, 2795
Alw21I GWGCWC 2 cut(s) 904, 1401
Alw26I GTCTC 4 cut(s) 1722, 1772, 2156, 2297
Alw44I GTGCAC 1 cut(s) 900
AlwI GGATC 6 cut(s) 248, 569, 608, 730, 1919, 2618
AlwNI CAGNNNCTG 5 cut(s) 415, 518, 1123, 1579, 2894
AoxI GGCC 5 cut(s) 48, 504, 1413, 1973, 2878
ApaLI GTGCAC 1 cut(s) 900
ApeKI GCWGC 9 cut(s) 80, 515, 972, 1132, 2138, 2356, 2510, 2615, 2737
ApoI RAATTY 7 cut(s) 148, 402, 556, 1536, 2185, 2459, 2689
ArsI GACNNNNNNTTYG 4 cut(s) 314, 346, 1850, 1882
AsiGI ACCGGT 1 cut(s) 889
Asp700I GAANNNNTTC 3 cut(s) 1677, 2322, 2382
AspLEI GCGC 2 cut(s) 536, 1035
AspS9I GGNCC 1 cut(s) 1414
AsuC2I CCSGG 1 cut(s) 1251
AsuHPI GGTGA 6 cut(s) 152, 270, 623, 948, 1499, 1833
AsuNHI GCTAGC 1 cut(s) 1517
BaeGI GKGCMC 1 cut(s) 904
BaeI ACNNNNGTAYC 6 cut(s) 1167, 1200, 2430, 2463, 2655, 2688
BanI GGYRCC 2 cut(s) 2858, 2897
BanII GRGCYC 1 cut(s) 1401
BarI GAAGNNNNNNTAC 4 cut(s) 1119, 1151, 2833, 2865
BauI CACGAG 2 cut(s) 903, 2280
Bbv12I GWGCWC 2 cut(s) 904, 1401
BbvI GCAGC 9 cut(s) 67, 502, 984, 1144, 2125, 2343, 2522, 2627, 2724
BccI CCATC 9 cut(s) 818, 1018, 1466, 1633, 1888, 2274, 2480, 2545, 2638
BceAI ACGGC 1 cut(s) 822
BciT130I CCWGG 2 cut(s) 1304, 2583
BcnI CCSGG 1 cut(s) 1251
BcoDI GTCTC 4 cut(s) 1722, 1772, 2156, 2297
BfaI CTAG 8 cut(s) 267, 369, 626, 969, 1518, 1934, 2210, 2289
BfmI CTRYAG 3 cut(s) 105, 423, 1969
BfoI RGCGCY 1 cut(s) 537
BfuAI ACCTGC 1 cut(s) 289
BglII AGATCT 2 cut(s) 1430, 1930
Bme1390I CCNGG 3 cut(s) 1251, 1304, 2583
BmgT120I GGNCC 1 cut(s) 1414
BmiI GGNNCC 7 cut(s) 69, 75, 456, 1095, 2598, 2860, 2899
BmrFI CCNGG 3 cut(s) 1251, 1304, 2583
BmrI ACTGGG 1 cut(s) 2655
BmsI GCATC 7 cut(s) 398, 857, 1006, 1281, 1447, 2285, 2479
BmtI GCTAGC 1 cut(s) 1521
BmuI ACTGGG 1 cut(s) 2655
BpmI CTGGAG 1 cut(s) 1200
Bpu10I CCTNAGC 2 cut(s) 1418, 1913
BpuEI CTTGAG 2 cut(s) 1721, 2189
BpuMI CCSGG 1 cut(s) 1251
BsaBI GATNNNNATC 1 cut(s) 1929
BsaHI GRCGYC 1 cut(s) 419
BsaI GGTCTC 1 cut(s) 2297
BsaJI CCNNGG 4 cut(s) 450, 978, 2581, 2881
BsaWI WCCGGW 1 cut(s) 889
BsaXI ACNNNNNCTCC 2 cut(s) 2763, 2793
Bsc4I CCNNNNNNNGG 9 cut(s) 518, 587, 820, 1029, 1123, 1256, 1257, 1423, 2486
Bse118I RCCGGY 2 cut(s) 64, 889
Bse1I ACTGG 5 cut(s) 724, 1117, 1128, 1183, 2650
Bse3DI GCAATG 1 cut(s) 1384
Bse8I GATNNNNATC 1 cut(s) 1929
BseBI CCWGG 2 cut(s) 1304, 2583
BseDI CCNNGG 4 cut(s) 450, 978, 2581, 2881
BseJI GATNNNNATC 1 cut(s) 1929
BseLI CCNNNNNNNGG 9 cut(s) 518, 587, 820, 1029, 1123, 1256, 1257, 1423, 2486
BseMI GCAATG 1 cut(s) 1384
BseMII CTCAG 7 cut(s) 810, 1041, 1624, 1904, 2028, 2231, 2856
BseNI ACTGG 5 cut(s) 724, 1117, 1128, 1183, 2650
BseRI GAGGAG 2 cut(s) 447, 692
BseSI GKGCMC 1 cut(s) 904
BseXI GCAGC 9 cut(s) 67, 502, 984, 1144, 2125, 2343, 2522, 2627, 2724
BshFI GGCC 5 cut(s) 50, 506, 1415, 1975, 2880
BshNI GGYRCC 2 cut(s) 2858, 2897
BshTI ACCGGT 1 cut(s) 889
BsiHKAI GWGCWC 2 cut(s) 904, 1401
BsiSI CCGG 5 cut(s) 65, 71, 77, 890, 1251
BslFI GGGAC 2 cut(s) 817, 1922
BslI CCNNNNNNNGG 9 cut(s) 518, 587, 820, 1029, 1123, 1256, 1257, 1423, 2486
BsmAI GTCTC 4 cut(s) 1722, 1772, 2156, 2297
BsmFI GGGAC 2 cut(s) 817, 1922
BsmI GAATGC 2 cut(s) 391, 1054
BsnI GGCC 5 cut(s) 50, 506, 1415, 1975, 2880
Bso31I GGTCTC 1 cut(s) 2297
Bsp1286I GDGCHC 2 cut(s) 904, 1401
Bsp1407I TGTACA 1 cut(s) 2068
BspACI CCGC 7 cut(s) 1618, 1891, 1903, 1982, 2034, 2354, 2480
BspANI GGCC 5 cut(s) 50, 506, 1415, 1975, 2880
BspCNI CTCAG 7 cut(s) 811, 1042, 1623, 1905, 2029, 2232, 2857
BspHI TCATGA 2 cut(s) 1138, 2403
BspLI GGNNCC 7 cut(s) 69, 75, 456, 1095, 2598, 2860, 2899
BspMAI CTGCAG 1 cut(s) 427
BspMI ACCTGC 1 cut(s) 289
BspOI GCTAGC 1 cut(s) 1521
BspPI GGATC 6 cut(s) 248, 569, 608, 730, 1919, 2618
BspT107I GGYRCC 2 cut(s) 2858, 2897
BspTNI GGTCTC 1 cut(s) 2297
BsrBI CCGCTC 1 cut(s) 1903
BsrDI GCAATG 1 cut(s) 1384
BsrFI RCCGGY 2 cut(s) 64, 889
BsrGI TGTACA 1 cut(s) 2068
BsrI ACTGG 5 cut(s) 724, 1117, 1128, 1183, 2650
BssAI RCCGGY 2 cut(s) 64, 889
BssECI CCNNGG 4 cut(s) 450, 978, 2581, 2881
BssNI GRCGYC 1 cut(s) 419
BssSI CACGAG 2 cut(s) 903, 2280
BssT1I CCWWGG 2 cut(s) 450, 978
Bst2BI CACGAG 2 cut(s) 903, 2280
Bst2UI CCWGG 2 cut(s) 1304, 2583
Bst4CI ACNGT 8 cut(s) 102, 220, 623, 1005, 1331, 1450, 1571, 1601
Bst6I CTCTTC 5 cut(s) 484, 517, 1947, 2600, 2830
BstACI GRCGYC 1 cut(s) 419
BstAUI TGTACA 1 cut(s) 2068
BstC8I GCNNGC 3 cut(s) 9, 1519, 2499
BstEII GGTNACC 1 cut(s) 1112
BstENI CCTNNNNNAGG 1 cut(s) 818
BstH2I RGCGCY 1 cut(s) 537
BstHHI GCGC 2 cut(s) 536, 1035
BstMAI GTCTC 4 cut(s) 1722, 1772, 2156, 2297
BstMWI GCNNNNNNNGC 9 cut(s) 80, 86, 512, 972, 1039, 1226, 1815, 2033, 2507
BstNI CCWGG 2 cut(s) 1304, 2583
BstNSI RCATGY 2 cut(s) 987, 1735
BstPI GGTNACC 1 cut(s) 1112
BstSCI CCNGG 3 cut(s) 1249, 1302, 2581
BstSFI CTRYAG 3 cut(s) 105, 423, 1969
BstSLI GKGCMC 1 cut(s) 904
BstV1I GCAGC 9 cut(s) 67, 502, 984, 1144, 2125, 2343, 2522, 2627, 2724
BstX2I RGATCY 4 cut(s) 574, 722, 1430, 1930
BstYI RGATCY 4 cut(s) 574, 722, 1430, 1930
BsuRI GGCC 5 cut(s) 50, 506, 1415, 1975, 2880
BtsI GCAGTG 1 cut(s) 1825
BtsIMutI CAGTG 3 cut(s) 717, 1121, 1825
BveI ACCTGC 1 cut(s) 289
Cac8I GCNNGC 3 cut(s) 9, 1519, 2499
CaiI CAGNNNCTG 5 cut(s) 415, 518, 1123, 1579, 2894
CciI TCATGA 2 cut(s) 1138, 2403
CfoI GCGC 2 cut(s) 536, 1035
Cfr10I RCCGGY 2 cut(s) 64, 889
Cfr13I GGNCC 1 cut(s) 1414
Csp6I GTAC 5 cut(s) 654, 1151, 1232, 2069, 2555
CspAI ACCGGT 1 cut(s) 889
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CviQI GTAC 5 cut(s) 654, 1151, 1232, 2069, 2555
DrdI GACNNNNNNGTC 1 cut(s) 708
DseDI GACNNNNNNGTC 1 cut(s) 708
EaeI YGGCCR 2 cut(s) 504, 2878
Eam1104I CTCTTC 5 cut(s) 484, 517, 1947, 2600, 2830
EarI CTCTTC 5 cut(s) 484, 517, 1947, 2600, 2830
Ecl136II GAGCTC 1 cut(s) 1399
Eco130I CCWWGG 2 cut(s) 450, 978
Eco24I GRGCYC 1 cut(s) 1401
Eco31I GGTCTC 1 cut(s) 2297
Eco32I GATATC 1 cut(s) 16
Eco53kI GAGCTC 1 cut(s) 1399
Eco57I CTGAAG 3 cut(s) 401, 627, 1692
Eco91I GGTNACC 1 cut(s) 1112
EcoICRI GAGCTC 1 cut(s) 1399
EcoNI CCTNNNNNAGG 1 cut(s) 818
EcoO109I RGGNCCY 1 cut(s) 1414
EcoO65I GGTNACC 1 cut(s) 1112
EcoRI GAATTC 1 cut(s) 402
EcoRII CCWGG 2 cut(s) 1302, 2581
EcoRV GATATC 1 cut(s) 16
EcoT14I CCWWGG 2 cut(s) 450, 978
EcoT22I ATGCAT 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 1401
ErhI CCWWGG 2 cut(s) 450, 978
FalI AAGNNNNNCTT 2 cut(s) 2499, 2531
FaqI GGGAC 2 cut(s) 817, 1922
FblI GTMKAC 1 cut(s) 104
FriOI GRGCYC 1 cut(s) 1401
FspBI CTAG 8 cut(s) 267, 369, 626, 969, 1518, 1934, 2210, 2289
GlaI GCGC 2 cut(s) 535, 1034
GsuI CTGGAG 1 cut(s) 1200
HaeII RGCGCY 1 cut(s) 537
HaeIII GGCC 5 cut(s) 50, 506, 1415, 1975, 2880
HapII CCGG 5 cut(s) 65, 71, 77, 890, 1251
HhaI GCGC 2 cut(s) 536, 1035
Hin1I GRCGYC 1 cut(s) 419
Hin6I GCGC 2 cut(s) 534, 1033
HinP1I GCGC 2 cut(s) 534, 1033
HincII GTYRAC 2 cut(s) 1723, 1858
HindII GTYRAC 2 cut(s) 1723, 1858
HinfI GANTC 9 cut(s) 244, 263, 841, 908, 1346, 1365, 1765, 2039, 2285
HpaI GTTAAC 1 cut(s) 1723
HpaII CCGG 5 cut(s) 65, 71, 77, 890, 1251
HphI GGTGA 6 cut(s) 152, 270, 623, 948, 1499, 1833
Hpy166II GTNNAC 7 cut(s) 105, 656, 902, 956, 1594, 1723, 1858
Hpy188III TCNNGA 9 cut(s) 605, 1139, 1700, 1928, 1952, 2282, 2387, 2404, 2426
Hpy8I GTNNAC 7 cut(s) 105, 656, 902, 956, 1594, 1723, 1858
Hpy99I CGWCG 4 cut(s) 320, 323, 326, 329
HpyAV CCTTC 7 cut(s) 153, 602, 1667, 2333, 2365, 2528, 2788
HpyCH4III ACNGT 8 cut(s) 102, 220, 623, 1005, 1331, 1450, 1571, 1601
HpyCH4IV ACGT 4 cut(s) 419, 600, 1268, 2011
HpyF10VI GCNNNNNNNGC 9 cut(s) 80, 86, 512, 972, 1039, 1226, 1815, 2033, 2507
HpySE526I ACGT 4 cut(s) 419, 600, 1268, 2011
Hsp92I GRCGYC 1 cut(s) 419
HspAI GCGC 2 cut(s) 534, 1033
KspAI GTTAAC 1 cut(s) 1723
LmnI GCTCC 5 cut(s) 73, 79, 460, 980, 2602
Lsp1109I GCAGC 9 cut(s) 67, 502, 984, 1144, 2125, 2343, 2522, 2627, 2724
LweI GCATC 7 cut(s) 398, 857, 1006, 1281, 1447, 2285, 2479
MaeI CTAG 8 cut(s) 267, 369, 626, 969, 1518, 1934, 2210, 2289
MaeII ACGT 4 cut(s) 419, 600, 1268, 2011
MaeIII GTNAC 6 cut(s) 1112, 1119, 1325, 1565, 2572, 2641
MbiI CCGCTC 1 cut(s) 1903
MfeI CAATTG 2 cut(s) 1308, 2783
MflI RGATCY 4 cut(s) 574, 722, 1430, 1930
MhlI GDGCHC 2 cut(s) 904, 1401
MlyI GAGTC 3 cut(s) 238, 1774, 2048
Mph1103I ATGCAT 1 cut(s) 391
MroXI GAANNNNTTC 3 cut(s) 1677, 2322, 2382
MslI CAYNNNNRTG 3 cut(s) 138, 1158, 1736
MspA1I CMGCKG 8 cut(s) 515, 1853, 2036, 2138, 2356, 2482, 2501, 2831
MspI CCGG 5 cut(s) 65, 71, 77, 890, 1251
MspR9I CCNGG 3 cut(s) 1251, 1304, 2583
MunI CAATTG 2 cut(s) 1308, 2783
Mva1269I GAATGC 2 cut(s) 391, 1054
MvaI CCWGG 2 cut(s) 1304, 2583
MwoI GCNNNNNNNGC 9 cut(s) 80, 86, 512, 972, 1039, 1226, 1815, 2033, 2507
NciI CCSGG 1 cut(s) 1251
NheI GCTAGC 1 cut(s) 1517
NlaIV GGNNCC 7 cut(s) 69, 75, 456, 1095, 2598, 2860, 2899
NmeAIII GCCGAG 1 cut(s) 2906
NmuCI GTSAC 3 cut(s) 1119, 1325, 2572
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 2 cut(s) 987, 1735
PagI TCATGA 2 cut(s) 1138, 2403
PaqCI CACCTGC 1 cut(s) 289
PcsI WCGNNNNNNNCGW 3 cut(s) 321, 324, 1407
PctI GAATGC 2 cut(s) 391, 1054
PdmI GAANNNNTTC 3 cut(s) 1677, 2322, 2382
PfeI GAWTC 6 cut(s) 263, 841, 908, 1346, 1365, 2285
PflMI CCANNNNNTGG 2 cut(s) 518, 1123
PinAI ACCGGT 1 cut(s) 889
PleI GAGTC 3 cut(s) 238, 1773, 2047
PpsI GAGTC 3 cut(s) 238, 1773, 2047
Psp124BI GAGCTC 1 cut(s) 1401
Psp6I CCWGG 2 cut(s) 1302, 2581
PspEI GGTNACC 1 cut(s) 1112
PspGI CCWGG 2 cut(s) 1302, 2581
PspN4I GGNNCC 7 cut(s) 69, 75, 456, 1095, 2598, 2860, 2899
PspPI GGNCC 1 cut(s) 1414
PstI CTGCAG 1 cut(s) 427
PstNI CAGNNNCTG 5 cut(s) 415, 518, 1123, 1579, 2894
PsuI RGATCY 4 cut(s) 574, 722, 1430, 1930
PvuII CAGCTG 5 cut(s) 515, 1853, 2138, 2501, 2831
RsaI GTAC 5 cut(s) 655, 1152, 1233, 2070, 2556
RsaNI GTAC 5 cut(s) 654, 1151, 1232, 2069, 2555
RseI CAYNNNNRTG 3 cut(s) 138, 1158, 1736
SacI GAGCTC 1 cut(s) 1401
Sau96I GGNCC 1 cut(s) 1414
SchI GAGTC 3 cut(s) 238, 1774, 2048
ScrFI CCNGG 3 cut(s) 1251, 1304, 2583
SduI GDGCHC 2 cut(s) 904, 1401
SfaNI GCATC 7 cut(s) 398, 857, 1006, 1281, 1447, 2285, 2479
SfcI CTRYAG 3 cut(s) 105, 423, 1969
SmiMI CAYNNNNRTG 3 cut(s) 138, 1158, 1736
SmlI CTYRAG 2 cut(s) 1700, 2168
SmoI CTYRAG 2 cut(s) 1700, 2168
SsiI CCGC 7 cut(s) 1618, 1891, 1903, 1982, 2034, 2354, 2480
SspMI CTAG 8 cut(s) 267, 369, 626, 969, 1518, 1934, 2210, 2289
SstI GAGCTC 1 cut(s) 1401
StyD4I CCNGG 3 cut(s) 1249, 1302, 2581
StyI CCWWGG 2 cut(s) 450, 978
TaaI ACNGT 8 cut(s) 102, 220, 623, 1005, 1331, 1450, 1571, 1601
TaiI ACGT 4 cut(s) 422, 603, 1271, 2014
TaqI TCGA 2 cut(s) 247, 2369
TatI WGTACW 2 cut(s) 1231, 2068
TauI GCSGC 3 cut(s) 1985, 2036, 2356
TfiI GAWTC 6 cut(s) 263, 841, 908, 1346, 1365, 2285
TscAI CASTG 3 cut(s) 724, 1128, 1825
TseFI GTSAC 3 cut(s) 1119, 1325, 2572
TseI GCWGC 9 cut(s) 80, 515, 972, 1132, 2138, 2356, 2510, 2615, 2737
Tsp45I GTSAC 3 cut(s) 1119, 1325, 2572
TspGWI ACGGA 2 cut(s) 1160, 1740
TspRI CASTG 3 cut(s) 724, 1128, 1825
Van91I CCANNNNNTGG 2 cut(s) 518, 1123
VneI GTGCAC 1 cut(s) 900
XagI CCTNNNNNAGG 1 cut(s) 818
XapI RAATTY 7 cut(s) 148, 402, 556, 1536, 2185, 2459, 2689
XceI RCATGY 2 cut(s) 987, 1735
XmiI GTMKAC 1 cut(s) 104
XmnI GAANNNNTTC 3 cut(s) 1677, 2322, 2382
XspI CTAG 8 cut(s) 267, 369, 626, 969, 1518, 1934, 2210, 2289
ZraI GACGTC 1 cut(s) 420
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.