MD05G1047200.v1.1

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005505
Physical Location & Seq
Forward (+)
7984608 .. 7997206
12599 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1047200.v1.1.491

Sequence Viewer

Length: 2898 bp
ATGGCAGATATTAGGAAGTGGTTTATGAAGTCGCACGACAAGGGAAACAGCCAGAAACCGGTAGTGACTACTTCACAGACTCCTTCAACAGCTAAGACAGAGCCTAAAGAATCAGCGCGTGGAGATCAAGACAACTCTGGCAGGAGGAAAACTAGCAAGTATTTTCCCCCAGATAAGCCAACAGATGATAAAGAAACATCTAAAGTTCCAGCCAAAAGAAAGCCTCACAAGGATCCTGATGAATCTATTAAACCCTCACCTGCAAAAAAAGCTCACAAAGTTATTGATGACGATGATGACGATGACGATTTTGTCTCGCCTAATTTGAAGAAGAAATCAGTTGATTCCACTCCTAGTAAGAAACTGAAAAGCACGTCTGGCGTGGGAATCCCACAGAAGGTTACCACTATTGATGAAGGTGGCGATGATGATGCAAAGGCTGCCAAATCACCCCTCAAGCCGGCTGGTAGGGGTCGTGGTGGAAGAGGTACTTCTGCAGGACCAACCGGTGGAAGAGGCAGAGGCGCTGGACGAGGCGGATTTATGAACTTTGGAGAAAGGAAAGATCCTCCACACAAAGGAGAAAAGGAAGTTCCTGAAGGTGCTCCAGATTGTTTAGCTGGCTTGACTTTTGTAATTAGTGGTACACTTGACAGTTTGGAAAGAGAAGAAGCAGAGGATTTGATTAAACGTCATGGCGGTCATATTACTGGATCTGTCAGCAAGAAAACGAATTATCTTTTATGCGATGAAGATATTGAGGGAAGGAAATCCTCTAAAGCTAAAGAACTTGGTACGGCTTTTCTTACTGAGGATGGATTGTTTGATATGATTCGGGCGTCTATCGGTGCTAAAGTACCTGTACACGAAGCAAAGAAATCTGTGGATGATGCTGCAGCAGCATCTTTGCCCAATAAAAGCCCTAAGAAAGTAACATTAAAGAATTTGACTGGAAGCTCGTTGGCATCAAGTGCATCTAGCAAACTGTTGCAGTCAGATGCCTCCCTTGCTAGGCATAAGAAGCAAACTACCGGCCATTCTACTTTTACTTGGACAGAAAAATACAGGCCAAAGGTTCCAAACGACATTATTGGGAATCAGTCATTGGTAAAGCAGCTTCATGATTGGTTGGCACATTGGAATGAGCAATTTCTTGATACTGGAAATAAGAAAAAGGGAAAAAACCCAACCAATTCTGGTGCAAAAAGGGCTGTACTTTTAAGTGGAACACCTGGTATAGGGAAAACGACGTCTGCAAAGTTAGTCAGTCAGATGCTTGGTTTCCAGGCAATTGAGGTAAATGCTAGTGACAGTCGTTGTAAATCTGATTCAAAGATTGAAAAGGGAATTGGTGGAAGCAATGCGAATTCGATTAAAGAGCTTGTCAGCAACAAGGCCCTAAGCAAGGATGGATCAAAGCATCCCAAAACTGTGCTGATTATGGATGAGGTTGATGGGATGTCTGCTGGAGATCGGGGAGGAGTTGCTGATCTTATTGCTAGCATAAAGATTTCAAAAATTCCTATTATCTGCATTTGTAATGATCGTTACAGTCAGAAACTGAAAAGTCTTGTGAACTACTGTTTGCTTCTCAGCTTTCACAAACCTACCAAGCAACAGATGGCAAAGAGGTTGATGCAAATTGCAAGTGCTGAAGGCCTTCAAGTTAATGAGATTGCTCTTGAGGAACTGGCAGAAAAGGTTAATGGAGACATGCGAATGGCTGTTAACCAATTGCAATATATGAGCCTCTCAATGTCTGTCATTAAATATGATGATGTACGGCAACGCTTATTAAGCAGTTCAAAGGATGAAGATATTTCACCATTTACAGCTGTTGACAAGCTGTTTGGTTTTAATGCTGGAAAGTTGCGAATGGATGAGCGAGTTGACCTGAGCATGAGTGATCCTGACCTAGTCCCTCTTCTTATCCAGGAAAACTATATCAACTACAGGCCAAGTTCGGCTGTAAAAGATGATAGTGGGATAAAACGTATGGACTTGATTGCCCATGCCGCTGAGTCTATTGGCAATGGAGATATTTTCAATGTACAGATTAGAAAATATCGGCAATGGCAGCTTTCCCAAAGTGCTTGTCTTTCATCCTCTATAATTCCTGCTGCATTGTTGCGGGGGCAAAGAGAGACACTTGAGCAGGGAGAAAGGAATTTTAATAGATTTGGAGGGTGGCTGGGAAAGAATTCAACGTTAGGGAAAAATCTTAGGCTCTTGGAAGATTTGCATGTTCATCTTCTTGCTTCTCGTGAATCTAGTTCGGGGAGGGAAACACTACGAGTTGAATACCTTTTTCTTCTTCTGAAGCGATTGACCGTGCCACTACGTGAGCTGCCTAAGGATGAAGCTGTGCAGGAAGTTGTGGAGTTCATGAATGCTTACTCCATTAGTCAGGATGACTTTGATACTATTGTGGAGTTATCCAAATATAAGGGGCACCCAAATCCGCTAGATGGCATACTACCTGCTGTAAAAGCTGCTTTGACAAAAGCATACAAAGAAGGAAGCAAAACAAGGATGGTACGAGCTGCAGATTTTGTTACTATTCCTGGAATGAAAAAGGCCCCTAAGAAGCGGATTGCTGCCCTTTTAGAACCATCTGATGTTGTAATTGGAGAGAACAATGACGATACTTTGGTTCAGAGTGAAGATGAAAATTCGTCAGATACAGAGGACTTGGAAGGCACTGCTGCTGGTGAGAAGCTGCAAAAGGAACTTCAGAGTTTGAACACAAAAGGAGTGCAAGTACAATTTGATTTGAAGGGTGCGCCAAATACAAGTGCAAAGAAGACACCAGCTGGAAGAGGTAGAGGTGGTTCCACTGCTGCCGTGGAGAAAAAAGGTGGCCGAGGTTCAGGAGCTGGTGCCAAGAGAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

966

Amino Acids

104.84

Weight (kDa)

9.34

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT PF00533 203 - 278 1.7e-16 BRCA1 C Terminus (BRCT) domain
PTCB-BRCT PF12738 218 - 266 5.4e-06 twin BRCT domain
AAA PF00004 405 - 515 1.4e-11 ATPase family associated with various cellular activities (AAA)
AAA_lid_RFC1 PF25361 539 - 595 1.3e-10 RFC1-like, AAA+ ATPase lid domain
RCF1-5-like_lid PF21960 543 - 584 3.4e-06 RCF1/5-like, AAA+ ATPase lid domain
RFC1 PF08519 676 - 838 6.7e-39 Replication factor RFC1 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 268
AasI GACNNNNNNGTC 1 cut(s) 311
AatII GACGTC 1 cut(s) 1253
Acc36I ACCTGC 2 cut(s) 268, 2488
AccB1I GGYRCC 2 cut(s) 2451, 2879
AccB7I CCANNNNNTGG 1 cut(s) 509
AccII CGCG 1 cut(s) 118
AciI CCGC 6 cut(s) 537, 699, 2016, 2131, 2462, 2590
AclI AACGTT 1 cut(s) 2207
AclWI GGATC 6 cut(s) 227, 240, 560, 721, 1420, 1901
AcoI YGGCCR 2 cut(s) 1033, 2860
AcsI RAATTY 6 cut(s) 943, 1366, 1518, 2167, 2200, 2671
AcuI CTGAAG 4 cut(s) 618, 1674, 2339, 2717
AcyI GRCGYC 2 cut(s) 839, 1250
AdeI CACNNNGTG 1 cut(s) 2342
AfiI CCNNNNNNNGG 9 cut(s) 58, 397, 460, 509, 578, 1011, 1238, 1405, 2468
AgeI ACCGGT 2 cut(s) 58, 506
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 4 cut(s) 1231, 1284, 1932, 2563
AjuI GAANNNNNNNTTGG 4 cut(s) 1088, 1120, 1332, 1364
Alw21I GWGCWC 1 cut(s) 607
Alw26I GTCTC 3 cut(s) 319, 1704, 2138
AlwI GGATC 6 cut(s) 227, 240, 560, 721, 1420, 1901
AlwNI CAGNNNCTG 2 cut(s) 1561, 2876
AoxI GGCC 7 cut(s) 1033, 1067, 1395, 1657, 1955, 2577, 2860
ApoI RAATTY 6 cut(s) 943, 1366, 1518, 2167, 2200, 2671
ArsI GACNNNNNNTTYG 4 cut(s) 1832, 1864, 2080, 2112
AsiGI ACCGGT 2 cut(s) 58, 506
Asp700I GAANNNNTTC 2 cut(s) 1659, 2200
AspLEI GCGC 3 cut(s) 118, 527, 2785
AspS9I GGNCC 3 cut(s) 500, 1396, 2578
AsuHPI GGTGA 4 cut(s) 249, 441, 1815, 2723
AsuNHI GCTAGC 1 cut(s) 1499
AvaII GGWCC 1 cut(s) 500
AxyI CCTNAGG 1 cut(s) 2352
BaeGI GKGCMC 1 cut(s) 2454
BaeI ACNNNNGTAYC 4 cut(s) 2412, 2445, 2637, 2670
BamHI GGATCC 1 cut(s) 232
BanI GGYRCC 2 cut(s) 2451, 2879
BarI GAAGNNNNNNTAC 2 cut(s) 1101, 1133
BauI CACGAG 1 cut(s) 2262
BbsI GAAGAC 1 cut(s) 2810
Bbv12I GWGCWC 1 cut(s) 607
BccI CCATC 7 cut(s) 809, 1403, 1448, 1615, 2462, 2527, 2620
BceAI ACGGC 3 cut(s) 813, 1799, 2828
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BciT130I CCWGG 4 cut(s) 1233, 1286, 1934, 2565
BcoDI GTCTC 3 cut(s) 319, 1704, 2138
BfaI CTAG 9 cut(s) 153, 354, 978, 1011, 1305, 1500, 1916, 2271, 2465
BfmI CTRYAG 4 cut(s) 495, 894, 1951, 2544
BfoI RGCGCY 1 cut(s) 528
BfuAI ACCTGC 2 cut(s) 268, 2488
Bme1390I CCNGG 4 cut(s) 1233, 1286, 1934, 2565
Bme18I GGWCC 1 cut(s) 500
BmgBI CACGTC 1 cut(s) 375
BmgT120I GGNCC 3 cut(s) 500, 1396, 2578
BmiI GGNNCC 6 cut(s) 234, 1077, 2453, 2580, 2833, 2881
BmrFI CCNGG 4 cut(s) 1233, 1286, 1934, 2565
BmsI GCATC 9 cut(s) 421, 880, 911, 974, 983, 988, 1263, 1429, 1626
BmtI GCTAGC 1 cut(s) 1503
BpiI GAAGAC 1 cut(s) 2810
BpmI CTGGAG 2 cut(s) 591, 1488
Bpu10I CCTNAGC 2 cut(s) 1400, 1895
BpuEI CTTGAG 3 cut(s) 440, 1703, 2171
BsaAI YACGTR 1 cut(s) 2342
BsaHI GRCGYC 2 cut(s) 839, 1250
BsaJI CCNNGG 2 cut(s) 2844, 2863
BsaWI WCCGGW 2 cut(s) 58, 506
BsaXI ACNNNNNCTCC 2 cut(s) 2745, 2775
Bsc4I CCNNNNNNNGG 9 cut(s) 58, 397, 460, 509, 578, 1011, 1238, 1405, 2468
Bse118I RCCGGY 4 cut(s) 58, 460, 506, 1031
Bse1I ACTGG 4 cut(s) 715, 955, 1165, 1695
Bse21I CCTNAGG 1 cut(s) 2352
Bse3DI GCAATG 3 cut(s) 1366, 2038, 2078
BseBI CCWGG 4 cut(s) 1233, 1286, 1934, 2565
BseDI CCNNGG 2 cut(s) 2844, 2863
BseLI CCNNNNNNNGG 9 cut(s) 58, 397, 460, 509, 578, 1011, 1238, 1405, 2468
BseMI GCAATG 3 cut(s) 1366, 2038, 2078
BseMII CTCAG 4 cut(s) 801, 1606, 1886, 2010
BseNI ACTGG 4 cut(s) 715, 955, 1165, 1695
BseRI GAGGAG 1 cut(s) 1494
BseSI GKGCMC 1 cut(s) 2454
BseYI CCCAGC 1 cut(s) 2191
BsgI GTGCAG 1 cut(s) 2387
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 7 cut(s) 1035, 1069, 1397, 1659, 1957, 2579, 2862
BshNI GGYRCC 2 cut(s) 2451, 2879
BshTI ACCGGT 2 cut(s) 58, 506
BsiHKAI GWGCWC 1 cut(s) 607
BsiSI CCGG 4 cut(s) 59, 461, 507, 1032
BslFI GGGAC 1 cut(s) 1904
BslI CCNNNNNNNGG 9 cut(s) 58, 397, 460, 509, 578, 1011, 1238, 1405, 2468
BsmAI GTCTC 3 cut(s) 319, 1704, 2138
BsmFI GGGAC 1 cut(s) 1904
BsmI GAATGC 1 cut(s) 2395
BsnI GGCC 7 cut(s) 1035, 1069, 1397, 1659, 1957, 2579, 2862
Bsp1286I GDGCHC 2 cut(s) 607, 2454
Bsp1407I TGTACA 2 cut(s) 862, 2050
Bsp143I GATC 9 cut(s) 124, 232, 565, 713, 1412, 1471, 1489, 1543, 1906
BspACI CCGC 6 cut(s) 537, 699, 2016, 2131, 2462, 2590
BspANI GGCC 7 cut(s) 1035, 1069, 1397, 1659, 1957, 2579, 2862
BspCNI CTCAG 4 cut(s) 802, 1605, 1887, 2011
BspFNI CGCG 1 cut(s) 118
BspHI TCATGA 2 cut(s) 1120, 2385
BspLI GGNNCC 6 cut(s) 234, 1077, 2453, 2580, 2833, 2881
BspMAI CTGCAG 3 cut(s) 499, 898, 2548
BspMI ACCTGC 2 cut(s) 268, 2488
BspOI GCTAGC 1 cut(s) 1503
BspPI GGATC 6 cut(s) 227, 240, 560, 721, 1420, 1901
BspT107I GGYRCC 2 cut(s) 2451, 2879
BsrDI GCAATG 3 cut(s) 1366, 2038, 2078
BsrFI RCCGGY 4 cut(s) 58, 460, 506, 1031
BsrGI TGTACA 2 cut(s) 862, 2050
BsrI ACTGG 4 cut(s) 715, 955, 1165, 1695
BssAI RCCGGY 4 cut(s) 58, 460, 506, 1031
BssECI CCNNGG 2 cut(s) 2844, 2863
BssMI GATC 9 cut(s) 124, 232, 565, 713, 1412, 1471, 1489, 1543, 1906
BssNI GRCGYC 2 cut(s) 839, 1250
BssSI CACGAG 1 cut(s) 2262
Bst2BI CACGAG 1 cut(s) 2262
Bst2UI CCWGG 4 cut(s) 1233, 1286, 1934, 2565
Bst4CI ACNGT 7 cut(s) 656, 987, 1313, 1432, 1553, 1583, 2332
Bst6I CTCTTC 4 cut(s) 478, 508, 1929, 2812
BstACI GRCGYC 2 cut(s) 839, 1250
BstAPI GCANNNNNTGC 2 cut(s) 440, 971
BstAUI TGTACA 2 cut(s) 862, 2050
BstBAI YACGTR 1 cut(s) 2342
BstC8I GCNNGC 3 cut(s) 462, 622, 1501
BstDSI CCRYGG 1 cut(s) 2844
BstEII GGTNACC 1 cut(s) 400
BstENI CCTNNNNNAGG 2 cut(s) 1236, 1403
BstFNI CGCG 1 cut(s) 118
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 3 cut(s) 118, 527, 2785
BstKTI GATC 9 cut(s) 127, 235, 568, 716, 1415, 1474, 1492, 1546, 1909
BstMAI GTCTC 3 cut(s) 319, 1704, 2138
BstMBI GATC 9 cut(s) 124, 232, 565, 713, 1412, 1471, 1489, 1543, 1906
BstNI CCWGG 4 cut(s) 1233, 1286, 1934, 2565
BstNSI RCATGY 2 cut(s) 1717, 2246
BstPI GGTNACC 1 cut(s) 400
BstSCI CCNGG 4 cut(s) 1231, 1284, 1932, 2563
BstSFI CTRYAG 4 cut(s) 495, 894, 1951, 2544
BstSLI GKGCMC 1 cut(s) 2454
BstUI CGCG 1 cut(s) 118
BstV2I GAAGAC 1 cut(s) 2810
BstX2I RGATCY 3 cut(s) 232, 565, 713
BstYI RGATCY 3 cut(s) 232, 565, 713
Bsu36I CCTNAGG 1 cut(s) 2352
BsuRI GGCC 7 cut(s) 1035, 1069, 1397, 1659, 1957, 2579, 2862
BtgI CCRYGG 1 cut(s) 2844
BtgZI GCGATG 2 cut(s) 438, 762
BtrI CACGTC 1 cut(s) 375
BtsI GCAGTG 2 cut(s) 2700, 2835
BtsIMutI CAGTG 2 cut(s) 2700, 2835
BveI ACCTGC 2 cut(s) 268, 2488
Cac8I GCNNGC 3 cut(s) 462, 622, 1501
CaiI CAGNNNCTG 2 cut(s) 1561, 2876
CciI TCATGA 2 cut(s) 1120, 2385
CfoI GCGC 3 cut(s) 118, 527, 2785
Cfr10I RCCGGY 4 cut(s) 58, 460, 506, 1031
Cfr13I GGNCC 3 cut(s) 500, 1396, 2578
CseI GACGC 1 cut(s) 828
CsiI ACCWGGT 1 cut(s) 1231
CspAI ACCGGT 2 cut(s) 58, 506
CviAII CATG 7 cut(s) 695, 1121, 1714, 1900, 2012, 2243, 2386
DpnI GATC 9 cut(s) 126, 234, 567, 715, 1414, 1473, 1491, 1545, 1908
DpnII GATC 9 cut(s) 124, 232, 565, 713, 1412, 1471, 1489, 1543, 1906
DraIII CACNNNGTG 1 cut(s) 2342
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
EaeI YGGCCR 2 cut(s) 1033, 2860
Eam1104I CTCTTC 4 cut(s) 478, 508, 1929, 2812
EarI CTCTTC 4 cut(s) 478, 508, 1929, 2812
EciI GGCGGA 1 cut(s) 552
Eco147I AGGCCT 1 cut(s) 1659
Eco47I GGWCC 1 cut(s) 500
Eco57I CTGAAG 4 cut(s) 618, 1674, 2339, 2717
Eco81I CCTNAGG 1 cut(s) 2352
Eco91I GGTNACC 1 cut(s) 400
EcoNI CCTNNNNNAGG 2 cut(s) 1236, 1403
EcoO109I RGGNCCY 2 cut(s) 1396, 2578
EcoO65I GGTNACC 1 cut(s) 400
EcoRI GAATTC 2 cut(s) 1366, 2200
EcoRII CCWGG 4 cut(s) 1231, 1284, 1932, 2563
FaeI CATG 7 cut(s) 698, 1124, 1717, 1903, 2015, 2246, 2389
FalI AAGNNNNNCTT 2 cut(s) 475, 507
FaqI GGGAC 1 cut(s) 1904
FatI CATG 7 cut(s) 694, 1120, 1713, 1899, 2011, 2242, 2385
FauI CCCGC 1 cut(s) 2124
FspBI CTAG 9 cut(s) 153, 354, 978, 1011, 1305, 1500, 1916, 2271, 2465
GlaI GCGC 3 cut(s) 117, 526, 2784
GsaI CCCAGC 1 cut(s) 2195
GsuI CTGGAG 2 cut(s) 591, 1488
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 7 cut(s) 1035, 1069, 1397, 1659, 1957, 2579, 2862
HapII CCGG 4 cut(s) 59, 461, 507, 1032
HgaI GACGC 1 cut(s) 828
HhaI GCGC 3 cut(s) 118, 527, 2785
Hin1I GRCGYC 2 cut(s) 839, 1250
Hin1II CATG 7 cut(s) 698, 1124, 1717, 1903, 2015, 2246, 2389
Hin6I GCGC 3 cut(s) 116, 525, 2783
HinP1I GCGC 3 cut(s) 116, 525, 2783
HincII GTYRAC 3 cut(s) 1729, 1840, 1891
HindII GTYRAC 3 cut(s) 1729, 1840, 1891
HpaI GTTAAC 1 cut(s) 1729
HpaII CCGG 4 cut(s) 59, 461, 507, 1032
HphI GGTGA 4 cut(s) 249, 441, 1815, 2723
Hpy166II GTNNAC 6 cut(s) 647, 865, 1576, 1729, 1840, 1891
Hpy188I TCNGA 9 cut(s) 997, 1272, 1327, 1557, 2319, 2617, 2658, 2680, 2736
Hpy8I GTNNAC 6 cut(s) 647, 865, 1576, 1729, 1840, 1891
Hpy99I CGWCG 1 cut(s) 1252
HpyCH4III ACNGT 7 cut(s) 656, 987, 1313, 1432, 1553, 1583, 2332
HpyCH4IV ACGT 6 cut(s) 374, 691, 1250, 1993, 2207, 2341
HpySE526I ACGT 6 cut(s) 374, 691, 1250, 1993, 2207, 2341
Hsp92I GRCGYC 2 cut(s) 839, 1250
Hsp92II CATG 7 cut(s) 698, 1124, 1717, 1903, 2015, 2246, 2389
HspAI GCGC 3 cut(s) 116, 525, 2783
KroI GCCGGC 1 cut(s) 460
KroNI GCCGGC 1 cut(s) 462
KspAI GTTAAC 1 cut(s) 1729
Kzo9I GATC 9 cut(s) 124, 232, 565, 713, 1412, 1471, 1489, 1543, 1906
LmnI GCTCC 2 cut(s) 610, 2873
LweI GCATC 9 cut(s) 421, 880, 911, 974, 983, 988, 1263, 1429, 1626
MabI ACCWGGT 1 cut(s) 1231
MaeI CTAG 9 cut(s) 153, 354, 978, 1011, 1305, 1500, 1916, 2271, 2465
MaeII ACGT 6 cut(s) 374, 691, 1250, 1993, 2207, 2341
MaeIII GTNAC 6 cut(s) 64, 400, 931, 1307, 1547, 2554
MalI GATC 9 cut(s) 126, 234, 567, 715, 1414, 1473, 1491, 1545, 1908
MboI GATC 9 cut(s) 124, 232, 565, 713, 1412, 1471, 1489, 1543, 1906
MfeI CAATTG 2 cut(s) 1290, 1733
MflI RGATCY 3 cut(s) 232, 565, 713
MhlI GDGCHC 2 cut(s) 607, 2454
MlyI GAGTC 2 cut(s) 73, 2030
MroNI GCCGGC 1 cut(s) 460
MroXI GAANNNNTTC 2 cut(s) 1659, 2200
MslI CAYNNNNRTG 2 cut(s) 1140, 1718
MspA1I CMGCKG 3 cut(s) 1835, 2018, 2813
MspI CCGG 4 cut(s) 59, 461, 507, 1032
MspR9I CCNGG 4 cut(s) 1233, 1286, 1934, 2565
MunI CAATTG 2 cut(s) 1290, 1733
Mva1269I GAATGC 1 cut(s) 2395
MvaI CCWGG 4 cut(s) 1233, 1286, 1934, 2565
MvnI CGCG 1 cut(s) 118
NaeI GCCGGC 1 cut(s) 462
NdeII GATC 9 cut(s) 124, 232, 565, 713, 1412, 1471, 1489, 1543, 1906
NgoMIV GCCGGC 1 cut(s) 460
NheI GCTAGC 1 cut(s) 1499
NlaIII CATG 7 cut(s) 698, 1124, 1717, 1903, 2015, 2246, 2389
NlaIV GGNNCC 6 cut(s) 234, 1077, 2453, 2580, 2833, 2881
NmeAIII GCCGAG 1 cut(s) 2888
NmuCI GTSAC 2 cut(s) 64, 1307
NspI RCATGY 2 cut(s) 1717, 2246
PagI TCATGA 2 cut(s) 1120, 2385
PaqCI CACCTGC 1 cut(s) 268
PceI AGGCCT 1 cut(s) 1659
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 1 cut(s) 2395
PdiI GCCGGC 1 cut(s) 462
PdmI GAANNNNTTC 2 cut(s) 1659, 2200
PfeI GAWTC 8 cut(s) 110, 242, 344, 387, 832, 1096, 1328, 2267
PflFI GACNNNGTC 1 cut(s) 1916
PflMI CCANNNNNTGG 1 cut(s) 509
PfoI TCCNGGA 2 cut(s) 1932, 2563
PinAI ACCGGT 2 cut(s) 58, 506
PleI GAGTC 2 cut(s) 73, 2029
PpsI GAGTC 2 cut(s) 73, 2029
Ppu21I YACGTR 1 cut(s) 2342
Psp1406I AACGTT 1 cut(s) 2207
Psp6I CCWGG 4 cut(s) 1231, 1284, 1932, 2563
PspEI GGTNACC 1 cut(s) 400
PspFI CCCAGC 1 cut(s) 2191
PspGI CCWGG 4 cut(s) 1231, 1284, 1932, 2563
PspN4I GGNNCC 6 cut(s) 234, 1077, 2453, 2580, 2833, 2881
PspPI GGNCC 3 cut(s) 500, 1396, 2578
PsrI GAACNNNNNNTAC 2 cut(s) 2815, 2847
PstI CTGCAG 3 cut(s) 499, 898, 2548
PstNI CAGNNNCTG 2 cut(s) 1561, 2876
PsuI RGATCY 3 cut(s) 232, 565, 713
PsyI GACNNNGTC 1 cut(s) 1916
PvuII CAGCTG 2 cut(s) 1835, 2813
RseI CAYNNNNRTG 2 cut(s) 1140, 1718
Sau3AI GATC 9 cut(s) 124, 232, 565, 713, 1412, 1471, 1489, 1543, 1906
Sau96I GGNCC 3 cut(s) 500, 1396, 2578
SchI GAGTC 2 cut(s) 73, 2030
ScrFI CCNGG 4 cut(s) 1233, 1286, 1934, 2565
SduI GDGCHC 2 cut(s) 607, 2454
SexAI ACCWGGT 1 cut(s) 1231
SfaNI GCATC 9 cut(s) 421, 880, 911, 974, 983, 988, 1263, 1429, 1626
SfcI CTRYAG 4 cut(s) 495, 894, 1951, 2544
SinI GGWCC 1 cut(s) 500
SmiMI CAYNNNNRTG 2 cut(s) 1140, 1718
SmlI CTYRAG 3 cut(s) 455, 1682, 2150
SmoI CTYRAG 3 cut(s) 455, 1682, 2150
SseBI AGGCCT 1 cut(s) 1659
SsiI CCGC 6 cut(s) 537, 699, 2016, 2131, 2462, 2590
SspMI CTAG 9 cut(s) 153, 354, 978, 1011, 1305, 1500, 1916, 2271, 2465
StuI AGGCCT 1 cut(s) 1659
StyD4I CCNGG 4 cut(s) 1231, 1284, 1932, 2563
TaaI ACNGT 7 cut(s) 656, 987, 1313, 1432, 1553, 1583, 2332
TaiI ACGT 6 cut(s) 377, 694, 1253, 1996, 2210, 2344
TaqI TCGA 1 cut(s) 1370
TatI WGTACW 4 cut(s) 862, 1213, 2050, 2761
TauI GCSGC 1 cut(s) 2018
TfiI GAWTC 8 cut(s) 110, 242, 344, 387, 832, 1096, 1328, 2267
TscAI CASTG 2 cut(s) 2707, 2842
TseFI GTSAC 2 cut(s) 64, 1307
Tsp45I GTSAC 2 cut(s) 64, 1307
TspRI CASTG 2 cut(s) 2707, 2842
Tth111I GACNNNGTC 1 cut(s) 1916
Van91I CCANNNNNTGG 1 cut(s) 509
VpaK11BI GGWCC 1 cut(s) 500
XagI CCTNNNNNAGG 2 cut(s) 1236, 1403
XapI RAATTY 6 cut(s) 943, 1366, 1518, 2167, 2200, 2671
XceI RCATGY 2 cut(s) 1717, 2246
XcmI CCANNNNNNNNNTGG 2 cut(s) 1618, 2842
XmnI GAANNNNTTC 2 cut(s) 1659, 2200
XspI CTAG 9 cut(s) 153, 354, 978, 1011, 1305, 1500, 1916, 2271, 2465
ZraI GACGTC 1 cut(s) 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.