Prupe.8G068000_v2.0.a1

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0005505
Physical Location & Seq
Forward (+)
10020457 .. 10034916
14460 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G068000.1

Sequence Viewer

Length: 2925 bp
ATGTCACAGGCAGATATTAGGAAGTGGTTTATGAAGTCGCACGACAAGGGCAATGGCAAGAAACCTGCGCCGACTACTTCACAGACTCCTTCTACAGCTAAGGAAGAGCCTAAAGAACCAGTGCATGGAGGTCAAGAGAATCCAGGCAGGAGGAAAACTAGCAAGTATTTTACCACAGATAAGCCAAAAGCTGAAAAAGAAACTGAAGTTCCAGCCAAAAGAAAGACTCACAAGGAACCTGATGAATCCGTGAAACCCTCACCTGCAAAAAAAGTTCACAAAGTTGTCGATGATGACGATGATTTTGTCTTACCTCATTCAAAGAAGAACTCAGTTGATGCGACTCCTAGTAAGAAATTGAAGAGTGCATCTGGCGTGGGAGTCCCACAGAAACTTACGGCTATTGATGAAGGTGGTGATAATGACGTCAAGGATGCTGAATCTCCTCACAAGCCTGGTGGTAAGGGTCGTGGTGGAAGAGGTACTTCGGCAGGACCAGCTGGTGGAAGAGGTACTTCGGCAGGACCAGCTGGTGGAAGAGGCAGGGGCGCTGGACGAGGTGGATTTATGAATTTCGGAGATAGAAAAGATCCTCCACACAAAGGAGAAAAGGAAGTTCCTGAAGGTGCTCCAGATTGTTTAGCTGGTTTAACTTTTGTAATTAGTGGTACACTTGACAGTTTGGAAAGAGAGGAAGCAGAGGAATTGATAAAACGTCATGGCGGTCGTATTACTGGATCTGTCAGCAAGAAAACGAACTATCTTTTATGTGACGAAGATATTGAGGGAAGGAAATCCTCTAAAGCCAAAGAACTTGGTACTGCTTTTCTCACCGAGGATGGACTGTTTGATATGATTCGGGCATCTATCCGTGCTAAAGTACCTGTGCAAGAGGCCAAGAAATCTGTGGATGATGCGGCAGCAGCACCTTTGCCCAAGAAAAGTCCTAATAAAGTAGCATCAAAGAAAGATTGTGCTGGAAGCTCCTTGGCATCAAGTGTATCCCACAAACAATTGGAGTCAGATGCTTCCCATGCTAGGCGTAAGAAGCAACCTACTGAGCATTCTGCCTCTACTTGGACAGAAAAATATAGGCCAAAGGTTCCAAATGACATTATCGGGAATCAGTCACTGGTAAAGCAGCTTCATGATTGGTTGGTACATTGGCATGAGCAATTTCTTGATACTGGAAATAAGAAAAAAGGCAAAAACCCAACCAATTCTAGTGCCAAAAAGGCTGTACTATTAAGTGGAACGCCTGGTATAGGGAAAACGACGTCCGCTAAGTTAGTCAGTCAGATGCTTGGTTTCCAGACAATTGAGGTAAATGCTAGTGACAGTCGTGGGAAAGCTGATTCCAAGATTGAAAAGGGAATTGGTGGAAGCAATGCAAATTCTATTAAAGAGCTTGTCAGCAACAAGGCCCTGAGCATGGATGGATTAAAGCATCCCAAAACTGTGCTAATTATGGATGAGGTTGATGGGATGTCTGCTGGAGATCGGGGAGGAGTTGCTGATCTTATTGCTAGCATAAAGATTTCTAAAATTCCTGTGATCTGCATTTGTAATGATCGTTACAGTCAGAAACTGAAAAGTCTTGTGAACTACTGTTTACTTCTCAGCTTTCGGAAACCTACTAAACAACAGATGGCAAAGAGGTTGATGCAAATTGCAAATGCTGAAGGACTTAAAGTTAATGAGATTGCTCTTGAGGAACTCGCAGAAAAAGTTAATGGAGACATGCGAATGGCAGTTAACCAGTTGCAGTATATGAGCCTATCAATGTCTGTGATTAAATATGATGATGTAAGGCAGCGCTTATTAAGCAGTGCAAAGGATGAAGATATTTCACCATTCACAGCTGTTGACAAGCTGTTTGGTTTTAATGCAGGAAAGTTGCGGATGGATGAGCGTGTTGACCTGAGCATGAGTGATCCTGATCTAGTCCCTCTTCTTATACAGGAAAATTATATTAACTATAGGCCAAGTTCGGCTGTCAAGGATGATAGTGGGATAAAACGCATGAACTTGATTGCCCATGCCGCTGAGTCTATTGGCAATGGGGATATTTTCAATGTACAGATTAGAAAATATCGGCAATGGCAGCTTTCCCAAAGTGCTTGTCTTTCATCCTCTATTTTTCCTGCTGCATTGTTGCGTGGGCAAAGAGAGACACTTGAGCAGGGAGAAAGGAATTTTAATAGATTTGGGGGGTGGCTGGGAAAGAACTCAACACTTGGAAAAAATTCGAGGCTCTTGGAAGATTTGCATGTCCATCTTCTCGCTTCTCGTGAATCTAGTTCGGGGAGGGAAACCCTGCGAGTTGAATACCTTTCTCTTCTTCTGAAACGATTGACCGTGCCACTCCGTGAACTTCCTAAGGATGAAGCTGTACACAAAGTTGTGGATTTCATGAATACTTACTCCATTAGTCAGGATGACTTTGATACTATTGTGGAGTTATCCAAATTTCAGGGGCACCCAAATCCTCTAGATGGGATTCAACCAGCTGTAAAGGCAGCGCTGACAAAAGCTTACAAAGAAGGCAGCAAAACAAGGATGGTACGAGCTGCAGATTTTGTCACTTTACCTGGAATGAAGAAGGCTCCTAAGAAGCGGATTGCTGCTATTTTAGAACCATCTGTTGATGTAATTGGAGAGAACAATGATGATACTTTGGTTGAGAGTGAAGAAGAAAATTCGTCAGATACAGAGGACTTAGAAGGTTCTGCGGCTGGTGAGAAACTGCAACAGGAACTTCAAAGTTTAAATACAAAAGGAGTGCATGTACAATTCGATTTGAAGGGTGCAACAAATTCAAGTGCAAAGAAGACACCAACGGGCAGGGGTAGAGGTGGTTCATCTGCCGCAGCTGAGAAAAAAGGAGGCCGAGGTTCAGGGCCTGGTGGAAAGAGAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

975

Amino Acids

105.67

Weight (kDa)

9.4

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 271
AatII GACGTC 2 cut(s) 429, 1280
Acc36I ACCTGC 2 cut(s) 73, 271
AccB1I GGYRCC 1 cut(s) 2478
AccB7I CCANNNNNTGG 3 cut(s) 125, 503, 533
AciI CCGC 8 cut(s) 723, 917, 1281, 1900, 2043, 2617, 2732, 2868
AclWI GGATC 3 cut(s) 584, 745, 1928
AcsI RAATTY 8 cut(s) 571, 1393, 1545, 2194, 2245, 2468, 2698, 2815
AcuI CTGAAG 3 cut(s) 225, 642, 1701
AcyI GRCGYC 2 cut(s) 426, 1277
AdeI CACNNNGTG 1 cut(s) 2369
AfeI AGCGCT 2 cut(s) 1817, 2523
AfiI CCNNNNNNNGG 9 cut(s) 125, 503, 533, 602, 1038, 1077, 1265, 1432, 2495
AgsI TTSAA 9 cut(s) 321, 361, 1367, 2074, 2327, 2504, 2762, 2803, 2820
AjnI CCWGG 5 cut(s) 142, 454, 1258, 2590, 2902
AjuI GAANNNNNNNTTGG 2 cut(s) 1359, 1391
Alw21I GWGCWC 1 cut(s) 631
Alw26I GTCTC 2 cut(s) 1731, 2165
AlwI GGATC 3 cut(s) 584, 745, 1928
AlwNI CAGNNNCTG 3 cut(s) 1132, 1588, 2903
Aor51HI AGCGCT 2 cut(s) 1817, 2523
AoxI GGCC 6 cut(s) 894, 1094, 1422, 1982, 2887, 2900
ApoI RAATTY 8 cut(s) 571, 1393, 1545, 2194, 2245, 2468, 2698, 2815
ArsI GACNNNNNNTTYG 6 cut(s) 287, 319, 1859, 1891, 2107, 2139
Asp700I GAANNNNTTC 1 cut(s) 2245
AspLEI GCGC 4 cut(s) 70, 551, 1818, 2524
AspS9I GGNCC 4 cut(s) 494, 524, 1423, 2900
AsuHPI GGTGA 5 cut(s) 252, 428, 823, 1842, 2750
AsuNHI GCTAGC 1 cut(s) 1526
AvaII GGWCC 2 cut(s) 494, 524
AxyI CCTNAGG 1 cut(s) 2379
BaeGI GKGCMC 1 cut(s) 2481
BaeI ACNNNNGTAYC 4 cut(s) 2439, 2472, 2664, 2697
BanI GGYRCC 1 cut(s) 2478
BarI GAAGNNNNNNTAC 2 cut(s) 1128, 1160
BauI CACGAG 1 cut(s) 2289
BbsI GAAGAC 1 cut(s) 2837
Bbv12I GWGCWC 1 cut(s) 631
BccI CCATC 9 cut(s) 833, 1430, 1475, 1642, 1897, 2283, 2489, 2554, 2647
BceAI ACGGC 1 cut(s) 414
BciT130I CCWGG 5 cut(s) 144, 456, 1260, 2592, 2904
BciVI GTATCC 1 cut(s) 1012
BcoDI GTCTC 2 cut(s) 1731, 2165
BfaI CTAG 9 cut(s) 159, 348, 1038, 1224, 1332, 1527, 1943, 2298, 2492
BfmI CTRYAG 3 cut(s) 93, 1978, 2571
BfoI RGCGCY 3 cut(s) 552, 1819, 2525
BfuAI ACCTGC 2 cut(s) 73, 271
BfuI GTATCC 1 cut(s) 1012
BglI GCCNNNNNGGC 1 cut(s) 1235
Bme1390I CCNGG 5 cut(s) 144, 456, 1260, 2592, 2904
Bme18I GGWCC 2 cut(s) 494, 524
BmgT120I GGNCC 4 cut(s) 494, 524, 1423, 2900
BmiI GGNNCC 4 cut(s) 237, 1104, 2480, 2607
BmrFI CCNGG 5 cut(s) 144, 456, 1260, 2592, 2904
BmtI GCTAGC 1 cut(s) 1530
BpiI GAAGAC 1 cut(s) 2837
BpmI CTGGAG 2 cut(s) 615, 1515
Bpu10I CCTNAGC 3 cut(s) 99, 1427, 1922
BpuEI CTTGAG 2 cut(s) 1730, 2198
BsaBI GATNNNNATC 1 cut(s) 1938
BsaHI GRCGYC 2 cut(s) 426, 1277
BsaJI CCNNGG 3 cut(s) 834, 987, 2890
BsaXI ACNNNNNCTCC 2 cut(s) 2772, 2802
Bsc4I CCNNNNNNNGG 9 cut(s) 125, 503, 533, 602, 1038, 1077, 1265, 1432, 2495
Bse1I ACTGG 5 cut(s) 119, 739, 1137, 1192, 1759
Bse21I CCTNAGG 1 cut(s) 2379
Bse3DI GCAATG 4 cut(s) 58, 1393, 2065, 2105
Bse8I GATNNNNATC 1 cut(s) 1938
BseBI CCWGG 5 cut(s) 144, 456, 1260, 2592, 2904
BseDI CCNNGG 3 cut(s) 834, 987, 2890
BseJI GATNNNNATC 1 cut(s) 1938
BseLI CCNNNNNNNGG 9 cut(s) 125, 503, 533, 602, 1038, 1077, 1265, 1432, 2495
BseMI GCAATG 4 cut(s) 58, 1393, 2065, 2105
BseMII CTCAG 7 cut(s) 345, 1050, 1418, 1633, 1913, 2037, 2865
BseNI ACTGG 5 cut(s) 119, 739, 1137, 1192, 1759
BseRI GAGGAG 2 cut(s) 435, 1521
BseSI GKGCMC 1 cut(s) 2481
BseYI CCCAGC 1 cut(s) 2218
Bsh1285I CGRYCG 1 cut(s) 727
BshFI GGCC 6 cut(s) 896, 1096, 1424, 1984, 2889, 2902
BshNI GGYRCC 1 cut(s) 2478
BsiEI CGRYCG 1 cut(s) 727
BsiHKAI GWGCWC 1 cut(s) 631
BslFI GGGAC 2 cut(s) 368, 1931
BslI CCNNNNNNNGG 9 cut(s) 125, 503, 533, 602, 1038, 1077, 1265, 1432, 2495
BsmAI GTCTC 2 cut(s) 1731, 2165
BsmFI GGGAC 2 cut(s) 368, 1931
BsmI GAATGC 1 cut(s) 1063
BsnI GGCC 6 cut(s) 896, 1096, 1424, 1984, 2889, 2902
Bsp1286I GDGCHC 2 cut(s) 631, 2481
Bsp1407I TGTACA 3 cut(s) 2077, 2392, 2788
Bsp143I GATC 8 cut(s) 589, 737, 1498, 1516, 1554, 1570, 1933, 1939
BspACI CCGC 8 cut(s) 723, 917, 1281, 1900, 2043, 2617, 2732, 2868
BspANI GGCC 6 cut(s) 896, 1096, 1424, 1984, 2889, 2902
BspCNI CTCAG 7 cut(s) 344, 1051, 1419, 1632, 1914, 2038, 2866
BspHI TCATGA 2 cut(s) 1147, 2412
BspLI GGNNCC 4 cut(s) 237, 1104, 2480, 2607
BspMAI CTGCAG 1 cut(s) 2575
BspMI ACCTGC 2 cut(s) 73, 271
BspOI GCTAGC 1 cut(s) 1530
BspPI GGATC 3 cut(s) 584, 745, 1928
BspQI GCTCTTC 1 cut(s) 99
BspT107I GGYRCC 1 cut(s) 2478
BsrDI GCAATG 4 cut(s) 58, 1393, 2065, 2105
BsrGI TGTACA 3 cut(s) 2077, 2392, 2788
BsrI ACTGG 5 cut(s) 119, 739, 1137, 1192, 1759
BssECI CCNNGG 3 cut(s) 834, 987, 2890
BssMI GATC 8 cut(s) 589, 737, 1498, 1516, 1554, 1570, 1933, 1939
BssNI GRCGYC 2 cut(s) 426, 1277
BssSI CACGAG 1 cut(s) 2289
BssT1I CCWWGG 1 cut(s) 987
Bst2BI CACGAG 1 cut(s) 2289
Bst2UI CCWGG 5 cut(s) 144, 456, 1260, 2592, 2904
Bst4CI ACNGT 7 cut(s) 680, 846, 1340, 1459, 1580, 1610, 2359
Bst6I CTCTTC 7 cut(s) 99, 356, 472, 502, 532, 1956, 2343
BstACI GRCGYC 2 cut(s) 426, 1277
BstAUI TGTACA 3 cut(s) 2077, 2392, 2788
BstC8I GCNNGC 1 cut(s) 1528
BstENI CCTNNNNNAGG 1 cut(s) 1263
BstH2I RGCGCY 3 cut(s) 552, 1819, 2525
BstHHI GCGC 4 cut(s) 70, 551, 1818, 2524
BstKTI GATC 8 cut(s) 592, 740, 1501, 1519, 1557, 1573, 1936, 1942
BstMAI GTCTC 2 cut(s) 1731, 2165
BstMBI GATC 8 cut(s) 589, 737, 1498, 1516, 1554, 1570, 1933, 1939
BstMCI CGRYCG 1 cut(s) 727
BstNI CCWGG 5 cut(s) 144, 456, 1260, 2592, 2904
BstNSI RCATGY 3 cut(s) 1744, 2273, 2789
BstSCI CCNGG 5 cut(s) 142, 454, 1258, 2590, 2902
BstSFI CTRYAG 3 cut(s) 93, 1978, 2571
BstSLI GKGCMC 1 cut(s) 2481
BstV2I GAAGAC 1 cut(s) 2837
BstX2I RGATCY 2 cut(s) 589, 737
BstYI RGATCY 2 cut(s) 589, 737
Bsu36I CCTNAGG 1 cut(s) 2379
BsuI GTATCC 1 cut(s) 1012
BsuRI GGCC 6 cut(s) 896, 1096, 1424, 1984, 2889, 2902
BtsI GCAGTG 1 cut(s) 1834
BtsIMutI CAGTG 3 cut(s) 126, 1130, 1834
BveI ACCTGC 2 cut(s) 73, 271
Cac8I GCNNGC 1 cut(s) 1528
CaiI CAGNNNCTG 3 cut(s) 1132, 1588, 2903
CciI TCATGA 2 cut(s) 1147, 2412
CfoI GCGC 4 cut(s) 70, 551, 1818, 2524
Cfr13I GGNCC 4 cut(s) 494, 524, 1423, 2900
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
DpnI GATC 8 cut(s) 591, 739, 1500, 1518, 1556, 1572, 1935, 1941
DpnII GATC 8 cut(s) 589, 737, 1498, 1516, 1554, 1570, 1933, 1939
DraI TTTAAA 1 cut(s) 2769
DraIII CACNNNGTG 1 cut(s) 2369
Eam1104I CTCTTC 7 cut(s) 99, 356, 472, 502, 532, 1956, 2343
EarI CTCTTC 7 cut(s) 99, 356, 472, 502, 532, 1956, 2343
Eco130I CCWWGG 1 cut(s) 987
Eco47I GGWCC 2 cut(s) 494, 524
Eco47III AGCGCT 2 cut(s) 1817, 2523
Eco57I CTGAAG 3 cut(s) 225, 642, 1701
Eco81I CCTNAGG 1 cut(s) 2379
EcoNI CCTNNNNNAGG 1 cut(s) 1263
EcoO109I RGGNCCY 2 cut(s) 1423, 2900
EcoRII CCWGG 5 cut(s) 142, 454, 1258, 2590, 2902
EcoT14I CCWWGG 1 cut(s) 987
ErhI CCWWGG 1 cut(s) 987
FalI AAGNNNNNCTT 4 cut(s) 469, 501, 499, 531
FaqI GGGAC 2 cut(s) 368, 1931
FspBI CTAG 9 cut(s) 159, 348, 1038, 1224, 1332, 1527, 1943, 2298, 2492
GlaI GCGC 4 cut(s) 69, 550, 1817, 2523
GsaI CCCAGC 1 cut(s) 2222
GsuI CTGGAG 2 cut(s) 615, 1515
HaeII RGCGCY 3 cut(s) 552, 1819, 2525
HaeIII GGCC 6 cut(s) 896, 1096, 1424, 1984, 2889, 2902
HhaI GCGC 4 cut(s) 70, 551, 1818, 2524
Hin1I GRCGYC 2 cut(s) 426, 1277
Hin6I GCGC 4 cut(s) 68, 549, 1816, 2522
HinP1I GCGC 4 cut(s) 68, 549, 1816, 2522
HincII GTYRAC 3 cut(s) 1756, 1867, 1918
HindII GTYRAC 3 cut(s) 1756, 1867, 1918
HindIII AAGCTT 1 cut(s) 2532
HpaI GTTAAC 1 cut(s) 1756
HphI GGTGA 5 cut(s) 252, 428, 823, 1842, 2750
Hpy166II GTNNAC 9 cut(s) 277, 671, 1603, 1613, 1756, 1867, 1918, 2372, 2395
Hpy188I TCNGA 7 cut(s) 578, 1024, 1299, 1584, 1629, 2346, 2707
Hpy8I GTNNAC 9 cut(s) 277, 671, 1603, 1613, 1756, 1867, 1918, 2372, 2395
Hpy99I CGWCG 1 cut(s) 1279
HpyAV CCTTC 9 cut(s) 99, 404, 617, 783, 1676, 2537, 2596, 2717, 2797
HpyCH4III ACNGT 7 cut(s) 680, 846, 1340, 1459, 1580, 1610, 2359
HpyCH4IV ACGT 3 cut(s) 426, 715, 1277
HpySE526I ACGT 3 cut(s) 426, 715, 1277
Hsp92I GRCGYC 2 cut(s) 426, 1277
HspAI GCGC 4 cut(s) 68, 549, 1816, 2522
KspAI GTTAAC 1 cut(s) 1756
Kzo9I GATC 8 cut(s) 589, 737, 1498, 1516, 1554, 1570, 1933, 1939
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 3 cut(s) 634, 989, 2611
MaeI CTAG 9 cut(s) 159, 348, 1038, 1224, 1332, 1527, 1943, 2298, 2492
MaeII ACGT 3 cut(s) 426, 715, 1277
MaeIII GTNAC 6 cut(s) 3, 770, 1128, 1334, 1574, 2581
MalI GATC 8 cut(s) 591, 739, 1500, 1518, 1556, 1572, 1935, 1941
MboI GATC 8 cut(s) 589, 737, 1498, 1516, 1554, 1570, 1933, 1939
MfeI CAATTG 2 cut(s) 1013, 1317
MflI RGATCY 2 cut(s) 589, 737
MhlI GDGCHC 2 cut(s) 631, 2481
MlyI GAGTC 6 cut(s) 79, 220, 337, 390, 1028, 2057
MroXI GAANNNNTTC 1 cut(s) 2245
MslI CAYNNNNRTG 2 cut(s) 1167, 1745
MspA1I CMGCKG 6 cut(s) 500, 530, 1862, 2045, 2510, 2873
MspR9I CCNGG 5 cut(s) 144, 456, 1260, 2592, 2904
MunI CAATTG 2 cut(s) 1013, 1317
Mva1269I GAATGC 1 cut(s) 1063
MvaI CCWGG 5 cut(s) 144, 456, 1260, 2592, 2904
NdeII GATC 8 cut(s) 589, 737, 1498, 1516, 1554, 1570, 1933, 1939
NheI GCTAGC 1 cut(s) 1526
NlaIV GGNNCC 4 cut(s) 237, 1104, 2480, 2607
NmeAIII GCCGAG 1 cut(s) 2915
NmuCI GTSAC 5 cut(s) 3, 770, 1128, 1334, 2581
NspI RCATGY 3 cut(s) 1744, 2273, 2789
PagI TCATGA 2 cut(s) 1147, 2412
PaqCI CACCTGC 1 cut(s) 271
PciSI GCTCTTC 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PctI GAATGC 1 cut(s) 1063
PdmI GAANNNNTTC 1 cut(s) 2245
PfeI GAWTC 8 cut(s) 139, 245, 440, 856, 1123, 1355, 2294, 2500
PflMI CCANNNNNTGG 3 cut(s) 125, 503, 533
PleI GAGTC 6 cut(s) 79, 220, 337, 389, 1027, 2056
PpsI GAGTC 6 cut(s) 79, 220, 337, 389, 1027, 2056
Psp6I CCWGG 5 cut(s) 142, 454, 1258, 2590, 2902
PspFI CCCAGC 1 cut(s) 2218
PspGI CCWGG 5 cut(s) 142, 454, 1258, 2590, 2902
PspN4I GGNNCC 4 cut(s) 237, 1104, 2480, 2607
PspPI GGNCC 4 cut(s) 494, 524, 1423, 2900
PsrI GAACNNNNNNTAC 2 cut(s) 2842, 2874
PstI CTGCAG 1 cut(s) 2575
PstNI CAGNNNCTG 3 cut(s) 1132, 1588, 2903
PsuI RGATCY 2 cut(s) 589, 737
PvuII CAGCTG 5 cut(s) 500, 530, 1862, 2510, 2873
RseI CAYNNNNRTG 2 cut(s) 1167, 1745
SapI GCTCTTC 1 cut(s) 99
Sau3AI GATC 8 cut(s) 589, 737, 1498, 1516, 1554, 1570, 1933, 1939
Sau96I GGNCC 4 cut(s) 494, 524, 1423, 2900
SchI GAGTC 6 cut(s) 79, 220, 337, 390, 1028, 2057
ScrFI CCNGG 5 cut(s) 144, 456, 1260, 2592, 2904
SduI GDGCHC 2 cut(s) 631, 2481
SfcI CTRYAG 3 cut(s) 93, 1978, 2571
SinI GGWCC 2 cut(s) 494, 524
SmiMI CAYNNNNRTG 2 cut(s) 1167, 1745
SmlI CTYRAG 2 cut(s) 1709, 2177
SmoI CTYRAG 2 cut(s) 1709, 2177
SsiI CCGC 8 cut(s) 723, 917, 1281, 1900, 2043, 2617, 2732, 2868
SspMI CTAG 9 cut(s) 159, 348, 1038, 1224, 1332, 1527, 1943, 2298, 2492
StyD4I CCNGG 5 cut(s) 142, 454, 1258, 2590, 2902
StyI CCWWGG 1 cut(s) 987
TaaI ACNGT 7 cut(s) 680, 846, 1340, 1459, 1580, 1610, 2359
TaiI ACGT 3 cut(s) 429, 718, 1280
TaqI TCGA 3 cut(s) 288, 2249, 2796
TatI WGTACW 4 cut(s) 1240, 2077, 2392, 2788
TauI GCSGC 4 cut(s) 920, 2045, 2735, 2870
TfiI GAWTC 8 cut(s) 139, 245, 440, 856, 1123, 1355, 2294, 2500
TscAI CASTG 3 cut(s) 126, 1137, 1834
TseFI GTSAC 5 cut(s) 3, 770, 1128, 1334, 2581
Tsp45I GTSAC 5 cut(s) 3, 770, 1128, 1334, 2581
TspGWI ACGGA 3 cut(s) 238, 860, 2357
TspRI CASTG 3 cut(s) 126, 1137, 1834
Van91I CCANNNNNTGG 3 cut(s) 125, 503, 533
VpaK11BI GGWCC 2 cut(s) 494, 524
XagI CCTNNNNNAGG 1 cut(s) 1263
XapI RAATTY 8 cut(s) 571, 1393, 1545, 2194, 2245, 2468, 2698, 2815
XbaI TCTAGA 1 cut(s) 2491
XceI RCATGY 3 cut(s) 1744, 2273, 2789
XcmI CCANNNNNNNNNTGG 1 cut(s) 904
XmnI GAANNNNTTC 1 cut(s) 2245
XspI CTAG 9 cut(s) 159, 348, 1038, 1224, 1332, 1527, 1943, 2298, 2492
ZraI GACGTC 2 cut(s) 427, 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.