pycom02g21030

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005505
Physical Location & Seq
Forward (+)
19099294 .. 19106396
7103 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g21030.1

Sequence Viewer

Length: 1461 bp
ATGCTTGGTTTCCAGGCAATTGAGGTAAATGCTAGTGACAGTCGTGGGAAAGCTGATTCAAAGATTGAAAAGGGAATTGGTGGAAGCAATGCAAACTCGATTAAAGAACTTGTCAGCAACAAGGCCCTGAGCATGGATGGATCAAGGCATCCCAAAACTGTGCTGATTATGGATGAGGTTGATGGGATGTCTGCTGGAGATCGGGGTGGAGTTGCTGATCTTATTGCTAGCATAAAGATTTCAAAAATTCCTATTATCTGCATTTGTAATGATCGTTATAGTCAGAAACTGAAAAGTCTTGTGAACTACTGTTTGCTTCTCAACTTTCGCAAACCTACCAAGCAGCAGATGGCAAAGAGGTTGATGCAAGTTGCAAATGCTGAAGGCCTTCAAGTTAATGAGATTGCTCTTGAGGAACTTGCAGAAAAAGTTAATGGAGACATGCGAATGGCAGTTAACCAATTGCAATATATGAGCCTCTCAATGTCAATCATTAAATATGATGATGTACGGCAGCGCTTATTAAGCAGTTCAAAGGATGAAGATATTTCACCATTCACAGCTGTTGACAAGCTGTTTGGTTTTAACGCTGGAAAGTTGCGAATGGATGAGCGTGTTGACCTGAGCATGAGTGATCCTGACCTAGTCCCTCTTCTAATCCAGGAAAATTATATCAACTATAGGCCAAGTTCGGCTGTTAAAGATGATAGTGGGATAAAACGTATGAACTTGATTGCCCGTGCCGCTGAGTCTATTGGCAATGGAGATATTTTCAATGTACAGATTAGAAAATATCGGCAGTGGCAGCTTTCCCAAAGTGCTTGTCTGTCATCTTCTATAATTCCTGCTGCATTGTTGCGGGGGCAGAGAGAGACACTTGAGCAGGGAGAAAGGAATTTTAATAGATTTGGAGGGTGGCTGGGAAAGAACTCAACATTAGGAAAAAATCTTAGGCTCTTGGAAGATTTGCATGTCCATCTTCTTGCTTCTCGTGAATCTAGTTCGGGGAGGGAAACACTGCGAGTTGAATACCTTTCTCTTCTTCTGAAACGATTGACCGTGCCTCTCCGTGAACTGCCTAAGGATGAAGCTGTGCAGGAAGTTGTGGAGTTCATGAATACTTACTGCATTAGTCAGGATGACTTTGATACTATTGTGGAGTTATCCAAATTTCAGGGGCATCCAAATCCGCTAGATGGCATACTGCCTGCTGTAAAATCAGCTCTGACAAAAGCATACAAAGAAGGAAGCAAAACAAGGATGGTACGAGCTGCAGATTTTGTTACTATTCCTGGAATGAAAAAGGTCCCTAAGAAGAGGATTGCTGTGCTTTTAGAACCATCTGATGATGTCATTGGTGAGAACAATGATGATACTTTTGTTCAGAGTGATGATGAAAATTCGTCAGATACAGAGGACTTGGGTAAGTTTTTCTGTCATCATTATGCTGCTCCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

487

Amino Acids

54.13

Weight (kDa)

6.38

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_lid_RFC1 PF25361 115 - 171 5.9e-10 RFC1-like, AAA+ ATPase lid domain
RCF1-5-like_lid PF21960 119 - 160 7e-07 RCF1/5-like, AAA+ ATPase lid domain
RFC1 PF08519 252 - 414 1.6e-40 Replication factor RFC1 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 744, 859, 1190
AclWI GGATC 2 cut(s) 148, 629
AcsI RAATTY 4 cut(s) 246, 895, 1169, 1399
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 3 cut(s) 510, 780, 1266
AfeI AGCGCT 1 cut(s) 518
AfiI CCNNNNNNNGG 2 cut(s) 133, 1196
AgsI TTSAA 7 cut(s) 60, 68, 243, 392, 534, 775, 1028
AjnI CCWGG 3 cut(s) 12, 660, 1291
AjuI GAANNNNNNNTTGG 2 cut(s) 60, 92
AluBI AGCT 7 cut(s) 53, 563, 574, 808, 1091, 1223, 1271
AluI AGCT 7 cut(s) 53, 563, 574, 808, 1091, 1223, 1271
Alw26I GTCTC 2 cut(s) 432, 866
AlwI GGATC 2 cut(s) 148, 629
AlwNI CAGNNNCTG 1 cut(s) 289
Aor51HI AGCGCT 1 cut(s) 518
AoxI GGCC 3 cut(s) 123, 385, 683
ApeKI GCWGC 6 cut(s) 343, 514, 805, 848, 1271, 1448
ApoI RAATTY 4 cut(s) 246, 895, 1169, 1399
ArsI GACNNNNNNTTYG 4 cut(s) 560, 592, 808, 840
Asp700I GAANNNNTTC 1 cut(s) 387
AspLEI GCGC 1 cut(s) 519
AspS9I GGNCC 2 cut(s) 124, 1306
AsuHPI GGTGA 2 cut(s) 543, 1370
AsuNHI GCTAGC 1 cut(s) 227
AvaII GGWCC 1 cut(s) 1306
AxyI CCTNAGG 1 cut(s) 1080
BaeI ACNNNNGTAYC 4 cut(s) 1140, 1173, 1365, 1398
BauI CACGAG 1 cut(s) 990
BbvI GCAGC 6 cut(s) 355, 526, 817, 835, 1258, 1435
BccI CCATC 7 cut(s) 131, 176, 343, 984, 1190, 1255, 1348
BceAI ACGGC 1 cut(s) 527
BciT130I CCWGG 3 cut(s) 14, 662, 1293
BcoDI GTCTC 2 cut(s) 432, 866
BfaI CTAG 5 cut(s) 33, 228, 644, 999, 1193
BfmI CTRYAG 2 cut(s) 679, 1272
BfoI RGCGCY 1 cut(s) 520
BisI GCNGC 7 cut(s) 344, 515, 744, 806, 849, 1272, 1449
BlsI GCNGC 7 cut(s) 345, 516, 745, 807, 850, 1273, 1450
Bme1390I CCNGG 3 cut(s) 14, 662, 1293
Bme18I GGWCC 1 cut(s) 1306
BmgT120I GGNCC 2 cut(s) 124, 1306
BmiI GGNNCC 1 cut(s) 1308
BmrFI CCNGG 3 cut(s) 14, 662, 1293
BmsI GCATC 3 cut(s) 157, 354, 1189
BmtI GCTAGC 1 cut(s) 231
BpmI CTGGAG 1 cut(s) 216
Bpu10I CCTNAGC 2 cut(s) 128, 623
BpuEI CTTGAG 2 cut(s) 431, 899
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 1196
Bse21I CCTNAGG 1 cut(s) 1080
Bse3DI GCAATG 2 cut(s) 94, 766
BseBI CCWGG 3 cut(s) 14, 662, 1293
BseLI CCNNNNNNNGG 2 cut(s) 133, 1196
BseMI GCAATG 2 cut(s) 94, 766
BseMII CTCAG 3 cut(s) 119, 614, 738
BseRI GAGGAG 1 cut(s) 1443
BseXI GCAGC 6 cut(s) 355, 526, 817, 835, 1258, 1435
BseYI CCCAGC 1 cut(s) 919
BsgI GTGCAG 1 cut(s) 1115
BshFI GGCC 3 cut(s) 125, 387, 685
BslFI GGGAC 2 cut(s) 632, 1292
BslI CCNNNNNNNGG 2 cut(s) 133, 1196
BsmAI GTCTC 2 cut(s) 432, 866
BsmFI GGGAC 2 cut(s) 632, 1292
BsnI GGCC 3 cut(s) 125, 387, 685
Bsp1407I TGTACA 1 cut(s) 778
Bsp143I GATC 5 cut(s) 140, 199, 217, 271, 634
BspACI CCGC 3 cut(s) 744, 859, 1190
BspANI GGCC 3 cut(s) 125, 387, 685
BspCNI CTCAG 3 cut(s) 120, 615, 739
BspHI TCATGA 1 cut(s) 1113
BspLI GGNNCC 1 cut(s) 1308
BspMAI CTGCAG 1 cut(s) 1276
BspOI GCTAGC 1 cut(s) 231
BspPI GGATC 2 cut(s) 148, 629
BsrDI GCAATG 2 cut(s) 94, 766
BsrGI TGTACA 1 cut(s) 778
BssMI GATC 5 cut(s) 140, 199, 217, 271, 634
BssSI CACGAG 1 cut(s) 990
Bst2BI CACGAG 1 cut(s) 990
Bst2UI CCWGG 3 cut(s) 14, 662, 1293
Bst4CI ACNGT 4 cut(s) 41, 160, 311, 1060
Bst6I CTCTTC 3 cut(s) 657, 1044, 1310
BstAUI TGTACA 1 cut(s) 778
BstC8I GCNNGC 2 cut(s) 229, 1209
BstDEI CTNAG 6 cut(s) 128, 623, 747, 950, 1080, 1311
BstH2I RGCGCY 1 cut(s) 520
BstHHI GCGC 1 cut(s) 519
BstKTI GATC 5 cut(s) 143, 202, 220, 274, 637
BstMAI GTCTC 2 cut(s) 432, 866
BstMBI GATC 5 cut(s) 140, 199, 217, 271, 634
BstMWI GCNNNNNNNGC 3 cut(s) 525, 743, 805
BstNI CCWGG 3 cut(s) 14, 662, 1293
BstNSI RCATGY 2 cut(s) 445, 974
BstSCI CCNGG 3 cut(s) 12, 660, 1291
BstSFI CTRYAG 2 cut(s) 679, 1272
BstV1I GCAGC 6 cut(s) 355, 526, 817, 835, 1258, 1435
Bsu36I CCTNAGG 1 cut(s) 1080
BsuRI GGCC 3 cut(s) 125, 387, 685
BtsI GCAGTG 2 cut(s) 806, 1016
BtsIMutI CAGTG 2 cut(s) 806, 1016
Cac8I GCNNGC 2 cut(s) 229, 1209
CaiI CAGNNNCTG 1 cut(s) 289
CciI TCATGA 1 cut(s) 1113
CfoI GCGC 1 cut(s) 519
Cfr13I GGNCC 2 cut(s) 124, 1306
Csp6I GTAC 3 cut(s) 509, 779, 1265
CviAII CATG 5 cut(s) 133, 442, 628, 971, 1114
CviQI GTAC 3 cut(s) 509, 779, 1265
DdeI CTNAG 6 cut(s) 128, 623, 747, 950, 1080, 1311
DpnI GATC 5 cut(s) 142, 201, 219, 273, 636
DpnII GATC 5 cut(s) 140, 199, 217, 271, 634
Eam1104I CTCTTC 3 cut(s) 657, 1044, 1310
EarI CTCTTC 3 cut(s) 657, 1044, 1310
Eco147I AGGCCT 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 1306
Eco47III AGCGCT 1 cut(s) 518
Eco57I CTGAAG 1 cut(s) 402
Eco81I CCTNAGG 1 cut(s) 1080
EcoO109I RGGNCCY 2 cut(s) 124, 1306
EcoRII CCWGG 3 cut(s) 12, 660, 1291
FaeI CATG 5 cut(s) 136, 445, 631, 974, 1117
FaqI GGGAC 2 cut(s) 632, 1292
FatI CATG 5 cut(s) 132, 441, 627, 970, 1113
FauI CCCGC 1 cut(s) 852
Fnu4HI GCNGC 7 cut(s) 344, 515, 744, 806, 849, 1272, 1449
Fsp4HI GCNGC 7 cut(s) 344, 515, 744, 806, 849, 1272, 1449
FspBI CTAG 5 cut(s) 33, 228, 644, 999, 1193
GlaI GCGC 1 cut(s) 518
GluI GCNGC 7 cut(s) 344, 515, 744, 806, 849, 1272, 1449
GsaI CCCAGC 1 cut(s) 923
GsuI CTGGAG 1 cut(s) 216
HaeII RGCGCY 1 cut(s) 520
HaeIII GGCC 3 cut(s) 125, 387, 685
HhaI GCGC 1 cut(s) 519
Hin1II CATG 5 cut(s) 136, 445, 631, 974, 1117
Hin6I GCGC 1 cut(s) 517
HinP1I GCGC 1 cut(s) 517
HincII GTYRAC 3 cut(s) 457, 568, 619
HindII GTYRAC 3 cut(s) 457, 568, 619
HinfI GANTC 3 cut(s) 56, 749, 995
HpaI GTTAAC 1 cut(s) 457
HphI GGTGA 2 cut(s) 543, 1370
Hpy166II GTNNAC 5 cut(s) 304, 457, 568, 619, 1073
Hpy188I TCNGA 6 cut(s) 285, 1047, 1227, 1345, 1386, 1408
Hpy188III TCNNGA 5 cut(s) 410, 638, 992, 1114, 1136
Hpy8I GTNNAC 5 cut(s) 304, 457, 568, 619, 1073
HpyAV CCTTC 3 cut(s) 377, 398, 1238
HpyCH4III ACNGT 4 cut(s) 41, 160, 311, 1060
HpyCH4IV ACGT 1 cut(s) 721
HpyF10VI GCNNNNNNNGC 3 cut(s) 525, 743, 805
HpyF3I CTNAG 6 cut(s) 128, 623, 747, 950, 1080, 1311
HpySE526I ACGT 1 cut(s) 721
Hsp92II CATG 5 cut(s) 136, 445, 631, 974, 1117
HspAI GCGC 1 cut(s) 517
KspAI GTTAAC 1 cut(s) 457
Kzo9I GATC 5 cut(s) 140, 199, 217, 271, 634
LmnI GCTCC 1 cut(s) 1456
Lsp1109I GCAGC 6 cut(s) 355, 526, 817, 835, 1258, 1435
LweI GCATC 3 cut(s) 157, 354, 1189
MaeI CTAG 5 cut(s) 33, 228, 644, 999, 1193
MaeII ACGT 1 cut(s) 721
MaeIII GTNAC 2 cut(s) 35, 1282
MalI GATC 5 cut(s) 142, 201, 219, 273, 636
MboI GATC 5 cut(s) 140, 199, 217, 271, 634
MboII GAAGA 8 cut(s) 554, 644, 825, 971, 974, 1031, 1034, 1327
MfeI CAATTG 2 cut(s) 18, 461
MluCI AATT 9 cut(s) 18, 75, 246, 461, 667, 840, 895, 1169, 1399
MlyI GAGTC 1 cut(s) 758
MroXI GAANNNNTTC 1 cut(s) 387
MslI CAYNNNNRTG 2 cut(s) 446, 1443
MspA1I CMGCKG 2 cut(s) 563, 746
MspR9I CCNGG 3 cut(s) 14, 662, 1293
MunI CAATTG 2 cut(s) 18, 461
MvaI CCWGG 3 cut(s) 14, 662, 1293
MwoI GCNNNNNNNGC 3 cut(s) 525, 743, 805
NdeII GATC 5 cut(s) 140, 199, 217, 271, 634
NheI GCTAGC 1 cut(s) 227
NlaIII CATG 5 cut(s) 136, 445, 631, 974, 1117
NlaIV GGNNCC 1 cut(s) 1308
NmuCI GTSAC 1 cut(s) 35
NspI RCATGY 2 cut(s) 445, 974
PagI TCATGA 1 cut(s) 1113
PceI AGGCCT 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 2 cut(s) 56, 995
PflFI GACNNNGTC 1 cut(s) 644
PfoI TCCNGGA 2 cut(s) 660, 1291
PkrI GCNGC 7 cut(s) 345, 516, 745, 807, 850, 1273, 1450
PleI GAGTC 1 cut(s) 757
PpsI GAGTC 1 cut(s) 757
PpuMI RGGWCCY 1 cut(s) 1306
Psp5II RGGWCCY 1 cut(s) 1306
Psp6I CCWGG 3 cut(s) 12, 660, 1291
PspFI CCCAGC 1 cut(s) 919
PspGI CCWGG 3 cut(s) 12, 660, 1291
PspN4I GGNNCC 1 cut(s) 1308
PspPI GGNCC 2 cut(s) 124, 1306
PspPPI RGGWCCY 1 cut(s) 1306
PstI CTGCAG 1 cut(s) 1276
PstNI CAGNNNCTG 1 cut(s) 289
PsyI GACNNNGTC 1 cut(s) 644
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 3 cut(s) 510, 780, 1266
RsaNI GTAC 3 cut(s) 509, 779, 1265
RseI CAYNNNNRTG 2 cut(s) 446, 1443
SatI GCNGC 7 cut(s) 344, 515, 744, 806, 849, 1272, 1449
Sau3AI GATC 5 cut(s) 140, 199, 217, 271, 634
Sau96I GGNCC 2 cut(s) 124, 1306
SchI GAGTC 1 cut(s) 758
ScrFI CCNGG 3 cut(s) 14, 662, 1293
SfaNI GCATC 3 cut(s) 157, 354, 1189
SfcI CTRYAG 2 cut(s) 679, 1272
SinI GGWCC 1 cut(s) 1306
SmiMI CAYNNNNRTG 2 cut(s) 446, 1443
SmlI CTYRAG 2 cut(s) 410, 878
SmoI CTYRAG 2 cut(s) 410, 878
Sse9I AATT 9 cut(s) 18, 75, 246, 461, 667, 840, 895, 1169, 1399
SseBI AGGCCT 1 cut(s) 387
SsiI CCGC 3 cut(s) 744, 859, 1190
SspMI CTAG 5 cut(s) 33, 228, 644, 999, 1193
StuI AGGCCT 1 cut(s) 387
StyD4I CCNGG 3 cut(s) 12, 660, 1291
TaaI ACNGT 4 cut(s) 41, 160, 311, 1060
TaiI ACGT 1 cut(s) 724
TaqI TCGA 1 cut(s) 98
TasI AATT 9 cut(s) 18, 75, 246, 461, 667, 840, 895, 1169, 1399
TatI WGTACW 1 cut(s) 778
TauI GCSGC 1 cut(s) 746
TfiI GAWTC 2 cut(s) 56, 995
TscAI CASTG 2 cut(s) 806, 1023
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 6 cut(s) 343, 514, 805, 848, 1271, 1448
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 7 cut(s) 555, 740, 1101, 1102, 1130, 1313, 1410
TspGWI ACGGA 1 cut(s) 1058
TspRI CASTG 2 cut(s) 806, 1023
Tth111I GACNNNGTC 1 cut(s) 644
VpaK11BI GGWCC 1 cut(s) 1306
XapI RAATTY 4 cut(s) 246, 895, 1169, 1399
XceI RCATGY 2 cut(s) 445, 974
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 5 cut(s) 33, 228, 644, 999, 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.