FvH4_3g28250

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005505
Physical Location & Seq
Reverse (-)
21220111 .. 21232114
12004 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28250.t1

Sequence Viewer

Length: 2907 bp
ATGTCGCAGGCTGATATCAGAAAGTGGTTTATGAAGTCCCACGACAAGGGCAATGCCTCCAAACCGGCTCCGGCTCCGGCTGCTTTGCCCAAACAGTCCACACAATCTCAAGAACAGAAAGTGCATGGAGGTCAAGAAAGTTCAGGCAGAAGGAAAACTAGCAAGCATTTTCCCCCGAATAAGTCAAAGCCGGATGAGGAAAAGGAAACGGTTCAAGTGCCAGCAAAGCGAAAGATTCAATCGGGTCCTGTTGAATCTAGCAGACCCTCGCCTGCGAAAAAAGTTCAGAAAGTTGAGGATGATGACGACGATGATTTTGTTATGCCTCAATCTAAGAAGAAAGAAGCCACGCCTAGCAAGAAACTGAAGACTGCATCTGGCAAGGGAATTCCACAGGAGCTGAAGTCTGCAGATGAAAGTGATGATGATGAAGCCAAGGGTTCTGAGGCTCCTCACAAATCTGGTGGTAGGGGTCGCGGTGGAAGAGGTAGTTCATCGGCTGCTCCAGCTGGTGGAAGAGGCAGAGGCGCTGGACGGGGTGGATTTATGAATTTTGGTGAAAGGAAAGATCCGCCGCACAAAGGGGAAAAGGATGTTCCTGAAGGTGATCCAAACTGTTTAGCTGGTTTAACTTTTGTAATAAGTGGTACACTTGATAGTTTGGAAAGAGAGGAGGCAGAAGATTTAATAAAACGCCATGGAGGCCGTATCACTGGATCTGTCAGCAAGAAAACGAGCTATCTTTTATGTGATGAAGATATTGAGGGCAGGAAATCCTCAAAAGCCAAAGAACTTGGGACGGCTTTCCTTACCGAGGATGGATTGTTTGATAAGATTCGTGCATCAATTCGTGCTAAAGCACCTGTGCAAGAAGCAAAGAAACTTGTGGATAGTGCCCAAGAAACATCTTTGCCTAATATAAGCCCTAAGAAAGTAGCATTAAAGAGTTCAGCAGATGGCACTAGCAGCTCCTTGGCATGTAGGGTATCCCAAAAACAGTCATCATCAGATGCTTCCCTTGCTAGGCGCAAAGAGAAAACTACAGAGCATTCTGCTTTGACATGGACAGAAAAATATAGACCAAAGGTTCCAAATGACATTATTGGTAATCAGTCACTGGTAAAACAGCTTCATGATTGGTTGGCACATTGGCATGAGCAATTCCTTGATACTGGAATTAAGAAAAAAGGCAATAAATCAAACAATTCGGGTGCTAAAAAAGCTTTATTATTAAGTGGAACACCTGGTATAGGGAAAACAACGTCAGCAAAGTTGGTCAGTCAGATGCTCGGTTTCGAGGCAATTGAGGTAAATGCAAGTGACAGTCGAGGGAAAGCTGATTCCAAGATTGTCAAGGGAGTCGGTGGAAGCAATTCAAATTCTGTTAAAGAGCTCATCAGCAATATGGCTCTAAGCATGGATAGATCTAAGCATCCAAAAACTGTGCTCATTATGGATGAGGTTGATGGAATGTCGGCTGGTGATCGGGGTGGAGTTGCTGATCTTATTGCCAGCATAAAGATTTCTAAAATTCCCATTATCTGCATTTGTAATGATCGTTACAGTCAGAAACTGAAAAGTCTTGTGAACTACTGTTTGCTTCTCAGCTTCCGCAAACCAACTAAGCAACAGATGGCAAAGAGGTTGAAGCAAGTTGCAAATGCTGAAGGTCTTCAAGTGAATGAGATTGCTCTCGAGGAACTTGCAGACAGAGTTAACGGAGACATGCGAATGGCACTTAACCAGTTGCATTACATGAGTCTGTCAATGTCTGTCATAAAATATGAAGATGTAAGGCAACGCCTGCGAAGCAGTGGAAAGGATGAGGACATTTCACCATTCACAGCTGTTGATAAGCTGTTTAATTTCAATGCTGGGAAGTTGCGGATGGATGAGCGGGTTGACCTGAGCATGAGTGACCCAGATCTAGTCCCTCTTCTTATTCAGGAAAATTATGTTAACTACAGGCCAAGTGCGGCTGCGAAGGATAATAGTGGTGTTGAACGTATGAACTTGATTGCCCGTGCGGCTGAGTCTATTGGCAATGGGGATATTATCAATGTACAAATTAGAAAATATCGCCAATGGCAACTTTCCCAAAGTGGTTCTCTGTCATCCAGCATAATTCCGGCTGCTTTGTTGCGCGGTCAAAGGGAGACGCTTGAGCAGGGAGAAAGAAATTTTAATAGATTCGGAGGGTGGCTGGGAAAGAACTCAACATTTGGCAAAAATCTGAGGCTCTTAGATGATTTGCATGTCCATCTTCTTGCATCTCGTGCATCTAGTTCAGGAAGGGAGACCCTCCGAGTTGAATACCTTCCGCTCCTTCTGAAACGATTATCTGTGCCACTGCGAAAATTACCGAAGGACGAAGCCGTTCAGGAAGTTGTGGAGTTCATGAATACTTACTCAATTAGTCAGGATGACTATGATACTATCGTCGAGTTATCAAAATTTCAGGGGCATCCAAATCCTCTGGAGGGCATCCAGCCGGCTGTAAAAGCAGCTCTGACAAAAGCATACAAAGAAGGTAGCAAATCAAGGGTACGAGTTGCAGAGTTTGTCACTTTCCCTGGAATGAAAAAGGCTCCTAAGAAGAGGATCGCAGCTATTTTAGAACCATCTGATGAAGCAATTGGAGAGAACAATGATGATACTTTGGTTCTAAGTGAAGAAGAAAATTCATCGGATGCAGAGGACTTGGAAAGCTCTGCTGTTGGGGAGAAGCTGCAGCAGGAACTTCAAAGTTTGAATAGAAAAGGAGTTCAAGTTCAATTGGATATGAAAGGTGCAGCAAATACAAGTGCCAAGAGGACACTGGCTGGAAGAGGTAAAGGTGCTTCTTCTGGTACCTCTGAGAAAAAAGGTGGCCGAGGTTCAGGGTCTGGTTCCAAAAGAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

969

Amino Acids

105.41

Weight (kDa)

9.49

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT PF00533 204 - 277 5.4e-17 BRCA1 C Terminus (BRCT) domain
PTCB-BRCT PF12738 219 - 267 4.7e-06 twin BRCT domain
AAA_22 PF13401 406 - 492 1.7e-07 AAA domain
AAA PF00004 409 - 519 8.3e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_RFC1 PF25361 543 - 599 1.1e-07 RFC1-like, AAA+ ATPase lid domain
RCF1-5-like_lid PF21960 547 - 588 9e-07 RCF1/5-like, AAA+ ATPase lid domain
RFC1 PF08519 680 - 842 2.3e-40 Replication factor RFC1 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2849
AccB1I GGYRCC 1 cut(s) 2849
AccB7I CCANNNNNTGG 1 cut(s) 512
AccBSI CCGCTC 2 cut(s) 1897, 2323
AccII CGCG 2 cut(s) 477, 2145
AclWI GGATC 4 cut(s) 563, 602, 724, 2609
AcoI YGGCCR 1 cut(s) 2869
AcsI RAATTY 7 cut(s) 387, 550, 1378, 1530, 2179, 2453, 2680
AcuI CTGAAG 4 cut(s) 386, 422, 621, 1686
AfaI GTAC 4 cut(s) 649, 2064, 2547, 2851
AfiI CCNNNNNNNGG 7 cut(s) 46, 512, 581, 814, 1023, 1250, 2480
AjnI CCWGG 2 cut(s) 1243, 2572
AloI GAACNNNNNNTCC 2 cut(s) 2754, 2786
Alw21I GWGCWC 2 cut(s) 1395, 1449
Alw26I GTCTC 3 cut(s) 1716, 2150, 2291
AlwI GGATC 4 cut(s) 563, 602, 724, 2609
AlwNI CAGNNNCTG 4 cut(s) 400, 1117, 1573, 2885
Ama87I CYCGRG 1 cut(s) 1694
AoxI GGCC 3 cut(s) 703, 1967, 2869
ApoI RAATTY 7 cut(s) 387, 550, 1378, 1530, 2179, 2453, 2680
ArsI GACNNNNNNTTYG 2 cut(s) 299, 331
Asp700I GAANNNNTTC 5 cut(s) 210, 1372, 1671, 2316, 2376
Asp718I GGTACC 1 cut(s) 2849
AspLEI GCGC 3 cut(s) 530, 1029, 2145
AspS9I GGNCC 1 cut(s) 245
AsuHPI GGTGA 4 cut(s) 569, 617, 1493, 1827
AvaI CYCGRG 1 cut(s) 1694
AvaII GGWCC 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 898
BaeI ACNNNNGTAYC 4 cut(s) 2424, 2457, 2646, 2679
BanI GGYRCC 1 cut(s) 2849
BanII GRGCYC 1 cut(s) 1395
BarI GAAGNNNNNNTAC 4 cut(s) 1113, 1145, 2824, 2856
BauI CACGAG 1 cut(s) 2274
BbsI GAAGAC 2 cut(s) 374, 1664
Bbv12I GWGCWC 2 cut(s) 1395, 1449
BccI CCATC 7 cut(s) 812, 950, 1460, 1627, 1882, 2268, 2629
BceAI ACGGC 3 cut(s) 690, 816, 2360
BcgI CGANNNNNNTGC 2 cut(s) 1685, 1719
BciT130I CCWGG 2 cut(s) 1245, 2574
BciVI GTATCC 1 cut(s) 997
BcoDI GTCTC 3 cut(s) 1716, 2150, 2291
BfaI CTAG 7 cut(s) 159, 258, 354, 963, 1023, 1928, 2283
BfmI CTRYAG 4 cut(s) 408, 1041, 1963, 2729
BfoI RGCGCY 1 cut(s) 531
BfuI GTATCC 1 cut(s) 997
BglI GCCNNNNNGGC 2 cut(s) 702, 2027
BglII AGATCT 2 cut(s) 1424, 1924
Bme1390I CCNGG 2 cut(s) 1245, 2574
Bme18I GGWCC 1 cut(s) 245
BmeT110I CYCGRG 1 cut(s) 1694
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 8 cut(s) 69, 75, 246, 450, 1089, 2589, 2851, 2890
BmrFI CCNGG 2 cut(s) 1245, 2574
BpiI GAAGAC 2 cut(s) 374, 1664
BpmI CTGGAG 2 cut(s) 489, 2498
Bpu10I CCTNAGC 1 cut(s) 1907
BpuEI CTTGAG 2 cut(s) 93, 2183
BsaI GGTCTC 1 cut(s) 2291
BsaJI CCNNGG 6 cut(s) 435, 697, 813, 972, 2572, 2872
BsaXI ACNNNNNCTCC 4 cut(s) 389, 419, 2754, 2784
Bsc4I CCNNNNNNNGG 7 cut(s) 46, 512, 581, 814, 1023, 1250, 2480
Bse118I RCCGGY 2 cut(s) 64, 2491
Bse1I ACTGG 5 cut(s) 718, 1122, 1177, 1744, 2823
Bse3DI GCAATG 2 cut(s) 58, 2050
BseBI CCWGG 2 cut(s) 1245, 2574
BseDI CCNNGG 6 cut(s) 435, 697, 813, 972, 2572, 2872
BseLI CCNNNNNNNGG 7 cut(s) 46, 512, 581, 814, 1023, 1250, 2480
BseMI GCAATG 2 cut(s) 58, 2050
BseMII CTCAG 6 cut(s) 435, 1618, 1898, 2022, 2225, 2847
BseNI ACTGG 5 cut(s) 718, 1122, 1177, 1744, 2823
BseRI GAGGAG 2 cut(s) 441, 686
BseSI GKGCMC 1 cut(s) 898
BseYI CCCAGC 2 cut(s) 1874, 2203
BsgI GTGCAG 1 cut(s) 2811
Bsh1236I CGCG 2 cut(s) 477, 2145
BshFI GGCC 3 cut(s) 705, 1969, 2871
BshNI GGYRCC 1 cut(s) 2849
BsiHKAI GWGCWC 2 cut(s) 1395, 1449
BsiHKCI CYCGRG 1 cut(s) 1694
BsiSI CCGG 6 cut(s) 65, 71, 77, 191, 2129, 2492
BslFI GGGAC 3 cut(s) 22, 811, 1916
BslI CCNNNNNNNGG 7 cut(s) 46, 512, 581, 814, 1023, 1250, 2480
BsmAI GTCTC 3 cut(s) 1716, 2150, 2291
BsmBI CGTCTC 1 cut(s) 2150
BsmFI GGGAC 3 cut(s) 22, 811, 1916
BsmI GAATGC 1 cut(s) 1048
BsnI GGCC 3 cut(s) 705, 1969, 2871
Bso31I GGTCTC 1 cut(s) 2291
BsoBI CYCGRG 1 cut(s) 1694
Bsp1286I GDGCHC 3 cut(s) 898, 1395, 1449
Bsp1407I TGTACA 1 cut(s) 2062
Bsp143I GATC 9 cut(s) 568, 607, 716, 1424, 1483, 1501, 1555, 1924, 2601
Bsp19I CCATGG 1 cut(s) 697
BspANI GGCC 3 cut(s) 705, 1969, 2871
BspCNI CTCAG 6 cut(s) 436, 1617, 1899, 2023, 2226, 2848
BspFNI CGCG 2 cut(s) 477, 2145
BspHI TCATGA 2 cut(s) 1132, 2397
BspLI GGNNCC 8 cut(s) 69, 75, 246, 450, 1089, 2589, 2851, 2890
BspMAI CTGCAG 2 cut(s) 412, 2733
BspPI GGATC 4 cut(s) 563, 602, 724, 2609
BspT107I GGYRCC 1 cut(s) 2849
BspTNI GGTCTC 1 cut(s) 2291
BsrBI CCGCTC 2 cut(s) 1897, 2323
BsrDI GCAATG 2 cut(s) 58, 2050
BsrFI RCCGGY 2 cut(s) 64, 2491
BsrGI TGTACA 1 cut(s) 2062
BsrI ACTGG 5 cut(s) 718, 1122, 1177, 1744, 2823
BssAI RCCGGY 2 cut(s) 64, 2491
BssECI CCNNGG 6 cut(s) 435, 697, 813, 972, 2572, 2872
BssMI GATC 9 cut(s) 568, 607, 716, 1424, 1483, 1501, 1555, 1924, 2601
BssSI CACGAG 1 cut(s) 2274
BssT1I CCWWGG 3 cut(s) 435, 697, 972
Bst2BI CACGAG 1 cut(s) 2274
Bst2UI CCWGG 2 cut(s) 1245, 2574
Bst4CI ACNGT 8 cut(s) 96, 211, 617, 999, 1325, 1444, 1565, 1595
Bst6I CTCTTC 5 cut(s) 478, 511, 1941, 2591, 2821
BstAPI GCANNNNNTGC 2 cut(s) 1804, 2276
BstAUI TGTACA 1 cut(s) 2062
BstC8I GCNNGC 7 cut(s) 9, 164, 222, 273, 1513, 1805, 2493
BstDSI CCRYGG 1 cut(s) 697
BstENI CCTNNNNNAGG 2 cut(s) 812, 1248
BstFNI CGCG 2 cut(s) 477, 2145
BstH2I RGCGCY 1 cut(s) 531
BstHHI GCGC 3 cut(s) 530, 1029, 2145
BstKTI GATC 9 cut(s) 571, 610, 719, 1427, 1486, 1504, 1558, 1927, 2604
BstMAI GTCTC 3 cut(s) 1716, 2150, 2291
BstMBI GATC 9 cut(s) 568, 607, 716, 1424, 1483, 1501, 1555, 1924, 2601
BstNI CCWGG 2 cut(s) 1245, 2574
BstNSI RCATGY 3 cut(s) 981, 1729, 2258
BstSCI CCNGG 2 cut(s) 1243, 2572
BstSFI CTRYAG 4 cut(s) 408, 1041, 1963, 2729
BstSLI GKGCMC 1 cut(s) 898
BstUI CGCG 2 cut(s) 477, 2145
BstV2I GAAGAC 2 cut(s) 374, 1664
BstX2I RGATCY 4 cut(s) 568, 716, 1424, 1924
BstYI RGATCY 4 cut(s) 568, 716, 1424, 1924
BsuI GTATCC 1 cut(s) 997
BsuRI GGCC 3 cut(s) 705, 1969, 2871
BtgI CCRYGG 1 cut(s) 697
BtsI GCAGTG 2 cut(s) 1819, 2348
BtsIMutI CAGTG 5 cut(s) 711, 1115, 1819, 2348, 2816
Cac8I GCNNGC 7 cut(s) 9, 164, 222, 273, 1513, 1805, 2493
CaiI CAGNNNCTG 4 cut(s) 400, 1117, 1573, 2885
CciI TCATGA 2 cut(s) 1132, 2397
CfoI GCGC 3 cut(s) 530, 1029, 2145
Cfr10I RCCGGY 2 cut(s) 64, 2491
Cfr13I GGNCC 1 cut(s) 245
CseI GACGC 1 cut(s) 2167
CsiI ACCWGGT 1 cut(s) 1243
Csp6I GTAC 4 cut(s) 648, 2063, 2546, 2850
CspCI CAANNNNNGTGG 2 cut(s) 445, 480
CviQI GTAC 4 cut(s) 648, 2063, 2546, 2850
DpnI GATC 9 cut(s) 570, 609, 718, 1426, 1485, 1503, 1557, 1926, 2603
DpnII GATC 9 cut(s) 568, 607, 716, 1424, 1483, 1501, 1555, 1924, 2601
EaeI YGGCCR 1 cut(s) 2869
Eam1104I CTCTTC 5 cut(s) 478, 511, 1941, 2591, 2821
EarI CTCTTC 5 cut(s) 478, 511, 1941, 2591, 2821
EciI GGCGGA 1 cut(s) 561
Ecl136II GAGCTC 1 cut(s) 1393
Eco130I CCWWGG 3 cut(s) 435, 697, 972
Eco24I GRGCYC 1 cut(s) 1395
Eco31I GGTCTC 1 cut(s) 2291
Eco32I GATATC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 1393
Eco57I CTGAAG 4 cut(s) 386, 422, 621, 1686
Eco88I CYCGRG 1 cut(s) 1694
EcoICRI GAGCTC 1 cut(s) 1393
EcoNI CCTNNNNNAGG 2 cut(s) 812, 1248
EcoO109I RGGNCCY 1 cut(s) 245
EcoRI GAATTC 1 cut(s) 387
EcoRII CCWGG 2 cut(s) 1243, 2572
EcoRV GATATC 1 cut(s) 16
EcoT14I CCWWGG 3 cut(s) 435, 697, 972
EcoT38I GRGCYC 1 cut(s) 1395
ErhI CCWWGG 3 cut(s) 435, 697, 972
Esp3I CGTCTC 1 cut(s) 2150
FaqI GGGAC 3 cut(s) 22, 811, 1916
FauI CCCGC 1 cut(s) 1890
FriOI GRGCYC 1 cut(s) 1395
FspBI CTAG 7 cut(s) 159, 258, 354, 963, 1023, 1928, 2283
GlaI GCGC 3 cut(s) 529, 1028, 2144
GsaI CCCAGC 2 cut(s) 1878, 2207
GsuI CTGGAG 2 cut(s) 489, 2498
HaeII RGCGCY 1 cut(s) 531
HaeIII GGCC 3 cut(s) 705, 1969, 2871
HapII CCGG 6 cut(s) 65, 71, 77, 191, 2129, 2492
HgaI GACGC 1 cut(s) 2167
HhaI GCGC 3 cut(s) 530, 1029, 2145
Hin6I GCGC 3 cut(s) 528, 1027, 2143
HinP1I GCGC 3 cut(s) 528, 1027, 2143
HincII GTYRAC 3 cut(s) 1717, 1903, 1960
HindII GTYRAC 3 cut(s) 1717, 1903, 1960
HindIII AAGCTT 1 cut(s) 1221
HinfI GANTC 8 cut(s) 235, 254, 835, 1340, 1359, 1759, 2033, 2190
HpaI GTTAAC 2 cut(s) 1717, 1960
HpaII CCGG 6 cut(s) 65, 71, 77, 191, 2129, 2492
HphI GGTGA 4 cut(s) 569, 617, 1493, 1827
Hpy166II GTNNAC 6 cut(s) 99, 650, 1588, 1717, 1903, 1960
Hpy8I GTNNAC 6 cut(s) 99, 650, 1588, 1717, 1903, 1960
Hpy99I CGWCG 2 cut(s) 311, 2444
HpyAV CCTTC 9 cut(s) 144, 596, 1661, 1978, 2286, 2327, 2336, 2359, 2522
HpyCH4III ACNGT 8 cut(s) 96, 211, 617, 999, 1325, 1444, 1565, 1595
HpyCH4IV ACGT 2 cut(s) 1262, 2005
HpySE526I ACGT 2 cut(s) 1262, 2005
HspAI GCGC 3 cut(s) 528, 1027, 2143
KpnI GGTACC 1 cut(s) 2853
KroI GCCGGC 1 cut(s) 2491
KroNI GCCGGC 1 cut(s) 2493
KspAI GTTAAC 2 cut(s) 1717, 1960
Kzo9I GATC 9 cut(s) 568, 607, 716, 1424, 1483, 1501, 1555, 1924, 2601
LmnI GCTCC 8 cut(s) 73, 79, 397, 454, 508, 974, 2328, 2593
MabI ACCWGGT 1 cut(s) 1243
MaeI CTAG 7 cut(s) 159, 258, 354, 963, 1023, 1928, 2283
MaeII ACGT 2 cut(s) 1262, 2005
MaeIII GTNAC 5 cut(s) 1113, 1319, 1559, 1916, 2563
MalI GATC 9 cut(s) 570, 609, 718, 1426, 1485, 1503, 1557, 1926, 2603
MbiI CCGCTC 2 cut(s) 1897, 2323
MboI GATC 9 cut(s) 568, 607, 716, 1424, 1483, 1501, 1555, 1924, 2601
MfeI CAATTG 3 cut(s) 1302, 2634, 2774
MflI RGATCY 4 cut(s) 568, 716, 1424, 1924
MhlI GDGCHC 3 cut(s) 898, 1395, 1449
MlyI GAGTC 3 cut(s) 1368, 1768, 2042
MroNI GCCGGC 1 cut(s) 2491
MroXI GAANNNNTTC 5 cut(s) 210, 1372, 1671, 2316, 2376
MslI CAYNNNNRTG 2 cut(s) 1152, 1730
MspA1I CMGCKG 2 cut(s) 509, 1847
MspI CCGG 6 cut(s) 65, 71, 77, 191, 2129, 2492
MspR9I CCNGG 2 cut(s) 1245, 2574
MunI CAATTG 3 cut(s) 1302, 2634, 2774
Mva1269I GAATGC 1 cut(s) 1048
MvaI CCWGG 2 cut(s) 1245, 2574
MvnI CGCG 2 cut(s) 477, 2145
NaeI GCCGGC 1 cut(s) 2493
NcoI CCATGG 1 cut(s) 697
NdeII GATC 9 cut(s) 568, 607, 716, 1424, 1483, 1501, 1555, 1924, 2601
NgoMIV GCCGGC 1 cut(s) 2491
NlaIV GGNNCC 8 cut(s) 69, 75, 246, 450, 1089, 2589, 2851, 2890
NmeAIII GCCGAG 1 cut(s) 2897
NmuCI GTSAC 4 cut(s) 1113, 1319, 1916, 2563
NspI RCATGY 3 cut(s) 981, 1729, 2258
PaeR7I CTCGAG 1 cut(s) 1694
PagI TCATGA 2 cut(s) 1132, 2397
PctI GAATGC 1 cut(s) 1048
PdiI GCCGGC 1 cut(s) 2493
PdmI GAANNNNTTC 5 cut(s) 210, 1372, 1671, 2316, 2376
PfeI GAWTC 5 cut(s) 235, 254, 835, 1340, 2190
PflMI CCANNNNNTGG 1 cut(s) 512
PleI GAGTC 3 cut(s) 1367, 1767, 2041
PpsI GAGTC 3 cut(s) 1367, 1767, 2041
PpuMI RGGWCCY 1 cut(s) 245
Psp124BI GAGCTC 1 cut(s) 1395
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 2 cut(s) 1243, 2572
PspFI CCCAGC 2 cut(s) 1874, 2203
PspGI CCWGG 2 cut(s) 1243, 2572
PspN4I GGNNCC 8 cut(s) 69, 75, 246, 450, 1089, 2589, 2851, 2890
PspPI GGNCC 1 cut(s) 245
PspPPI RGGWCCY 1 cut(s) 245
PstI CTGCAG 2 cut(s) 412, 2733
PstNI CAGNNNCTG 4 cut(s) 400, 1117, 1573, 2885
PsuI RGATCY 4 cut(s) 568, 716, 1424, 1924
PvuII CAGCTG 2 cut(s) 509, 1847
RsaI GTAC 4 cut(s) 649, 2064, 2547, 2851
RsaNI GTAC 4 cut(s) 648, 2063, 2546, 2850
RseI CAYNNNNRTG 2 cut(s) 1152, 1730
SacI GAGCTC 1 cut(s) 1395
Sau3AI GATC 9 cut(s) 568, 607, 716, 1424, 1483, 1501, 1555, 1924, 2601
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 3 cut(s) 1368, 1768, 2042
ScrFI CCNGG 2 cut(s) 1245, 2574
SduI GDGCHC 3 cut(s) 898, 1395, 1449
SexAI ACCWGGT 1 cut(s) 1243
SfcI CTRYAG 4 cut(s) 408, 1041, 1963, 2729
Sfr274I CTCGAG 1 cut(s) 1694
SinI GGWCC 1 cut(s) 245
SlaI CTCGAG 1 cut(s) 1694
SmiMI CAYNNNNRTG 2 cut(s) 1152, 1730
SmlI CTYRAG 3 cut(s) 108, 1694, 2162
SmoI CTYRAG 3 cut(s) 108, 1694, 2162
SspMI CTAG 7 cut(s) 159, 258, 354, 963, 1023, 1928, 2283
SstI GAGCTC 1 cut(s) 1395
StyD4I CCNGG 2 cut(s) 1243, 2572
StyI CCWWGG 3 cut(s) 435, 697, 972
TaaI ACNGT 8 cut(s) 96, 211, 617, 999, 1325, 1444, 1565, 1595
TaiI ACGT 2 cut(s) 1265, 2008
TaqI TCGA 4 cut(s) 1296, 1327, 1695, 2442
TatI WGTACW 1 cut(s) 2062
TauI GCSGC 3 cut(s) 577, 1979, 2030
TfiI GAWTC 5 cut(s) 235, 254, 835, 1340, 2190
TscAI CASTG 5 cut(s) 718, 1122, 1819, 2355, 2823
TseFI GTSAC 4 cut(s) 1113, 1319, 1916, 2563
Tsp45I GTSAC 4 cut(s) 1113, 1319, 1916, 2563
TspGWI ACGGA 1 cut(s) 1734
TspRI CASTG 5 cut(s) 718, 1122, 1819, 2355, 2823
Van91I CCANNNNNTGG 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 245
XagI CCTNNNNNAGG 2 cut(s) 812, 1248
XapI RAATTY 7 cut(s) 387, 550, 1378, 1530, 2179, 2453, 2680
XceI RCATGY 3 cut(s) 981, 1729, 2258
XcmI CCANNNNNNNNNTGG 1 cut(s) 2815
XhoI CTCGAG 1 cut(s) 1694
XmnI GAANNNNTTC 5 cut(s) 210, 1372, 1671, 2316, 2376
XspI CTAG 7 cut(s) 159, 258, 354, 963, 1023, 1928, 2283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.