pycom07g05580

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005505
Physical Location & Seq
Reverse (-)
4860763 .. 4865123
4361 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g05580.7

Sequence Viewer

Length: 1023 bp
ATGGATGAGCGAGTTGACCTGAGTATGAGTGATCCTGACCTAGTCCCTCTACTAATCCAGGAAAATTATATCAACTATAGGCCAAGTGCGGCTGTTAAAGATGATAGTGGGATTAAACGTATGAACTTGATTGCCCGTGCCGCTGAGTCTATTGGCAATGGAGATATTTTCAATGTACAGATTAGGAAATATCGGCAATGGCAGCTTTCCCAAAGTGCTTCTCTTTCATCCTCTATAATTCCTGCTGCGTTGTTGCGGGGGCAAAGAGAGACACTTGAGCAGGGAGAAAGGAATTTTAATAGATTTGGAGGGTGGCTGGGAAAGAACTCAACATTAGGAAAGAATCGTAGGCTCTTGGAAGATTTGCATGTCCATCTTCTTGCTTCTCGTGAATCTAGTTCGGGGAGGGAAACAGTGCGAGTTGAATACCTTTCTCTTCTTCTGAAACGATTGACCATGCCTCTCCGTGAACTGCCTAAGGATGAAGCAGTGCAGGAAGTTGTGGAGTTCATGAATACTTACTCCATTAGTCAGGATGACTTTGATACTATTGTGGAGTTATCCAAATATCAGGGGCATCCAAATCCGCTAGATGGCATAGTGCCTGCTGTAAAAGCAGCTCTGACAAAGGCATACAAAGAAGGAAGCAAAACAAGGATGGTACGAGCTGCAGATTTTGTCACTATTCCTGGAATGAAAAAGGCCCCTAAGAAGCGGATTGCTGCGCTTTTAGAACCATCTGATGATGCAATTGGAGAGAACATTGATGATACTTTGGTTCAGAGTGAAGATGAAAATTCGTCAGATACAGAGGACTTGGGTTCAGCTGTTGGTGAGAAGCTGCAAAAGGAACTGCAAAGTTTGAACACAAAAGGAGTGCAAGTACAATTCGATTTGAAGGGTGCGCCTAATACAAGTGCAAAGAAGACACCAGCTGGCAGGGGTAGAGGTGGTTCTGCTGCTGCTGCCAGCGTGAAAAAGGGCGGTCGAGGTTCAGGAGCTGGTGCCAAGAGAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

341

Amino Acids

37.22

Weight (kDa)

9.25

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1004
AciI CCGC 6 cut(s) 89, 141, 256, 587, 715, 984
AclWI GGATC 1 cut(s) 26
AcsI RAATTY 2 cut(s) 292, 796
AfaI GTAC 3 cut(s) 177, 663, 885
AfiI CCNNNNNNNGG 1 cut(s) 593
AgsI TTSAA 4 cut(s) 172, 425, 865, 898
AjnI CCWGG 2 cut(s) 57, 688
AluBI AGCT 7 cut(s) 205, 620, 668, 827, 841, 935, 1001
AluI AGCT 7 cut(s) 205, 620, 668, 827, 841, 935, 1001
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 1 cut(s) 26
AlwNI CAGNNNCTG 1 cut(s) 1001
AoxI GGCC 2 cut(s) 80, 702
ApeKI GCWGC 9 cut(s) 202, 245, 617, 668, 722, 841, 959, 962, 965
ApoI RAATTY 2 cut(s) 292, 796
AspLEI GCGC 2 cut(s) 727, 907
AspS9I GGNCC 1 cut(s) 703
AsuHPI GGTGA 1 cut(s) 845
AxyI CCTNAGG 1 cut(s) 477
BaeI ACNNNNGTAYC 4 cut(s) 537, 570, 762, 795
BanI GGYRCC 1 cut(s) 1004
BauI CACGAG 1 cut(s) 387
BbsI GAAGAC 1 cut(s) 932
BbvI GCAGC 9 cut(s) 214, 232, 629, 655, 709, 828, 946, 949, 952
BccI CCATC 4 cut(s) 381, 587, 652, 745
BciT130I CCWGG 2 cut(s) 59, 690
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 3 cut(s) 41, 396, 590
BfmI CTRYAG 2 cut(s) 76, 669
Bme1390I CCNGG 2 cut(s) 59, 690
BmgT120I GGNCC 1 cut(s) 703
BmiI GGNNCC 2 cut(s) 705, 1006
BmrFI CCNGG 2 cut(s) 59, 690
BmsI GCATC 2 cut(s) 586, 736
BpiI GAAGAC 1 cut(s) 932
BpuEI CTTGAG 1 cut(s) 296
BsaXI ACNNNNNCTCC 2 cut(s) 867, 897
Bsc4I CCNNNNNNNGG 1 cut(s) 593
Bse21I CCTNAGG 1 cut(s) 477
Bse3DI GCAATG 2 cut(s) 163, 203
BseBI CCWGG 2 cut(s) 59, 690
BseGI GGATG 6 cut(s) 10, 227, 487, 541, 577, 663
BseLI CCNNNNNNNGG 1 cut(s) 593
BseMI GCAATG 2 cut(s) 163, 203
BseMII CTCAG 2 cut(s) 11, 135
BseXI GCAGC 9 cut(s) 214, 232, 629, 655, 709, 828, 946, 949, 952
BseYI CCCAGC 1 cut(s) 316
BsgI GTGCAG 1 cut(s) 512
Bsh1285I CGRYCG 1 cut(s) 988
BshFI GGCC 2 cut(s) 82, 704
BshNI GGYRCC 1 cut(s) 1004
BsiEI CGRYCG 1 cut(s) 988
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 1 cut(s) 593
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 1 cut(s) 29
BsnI GGCC 2 cut(s) 82, 704
Bsp1407I TGTACA 1 cut(s) 175
Bsp143I GATC 1 cut(s) 31
BspACI CCGC 6 cut(s) 89, 141, 256, 587, 715, 984
BspANI GGCC 2 cut(s) 82, 704
BspCNI CTCAG 2 cut(s) 12, 136
BspHI TCATGA 1 cut(s) 510
BspLI GGNNCC 2 cut(s) 705, 1006
BspMAI CTGCAG 1 cut(s) 673
BspPI GGATC 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 1004
BsrDI GCAATG 2 cut(s) 163, 203
BsrGI TGTACA 1 cut(s) 175
BssMI GATC 1 cut(s) 31
BssSI CACGAG 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 387
Bst2UI CCWGG 2 cut(s) 59, 690
Bst4CI ACNGT 1 cut(s) 415
Bst6I CTCTTC 1 cut(s) 441
BstAUI TGTACA 1 cut(s) 175
BstC8I GCNNGC 3 cut(s) 606, 937, 970
BstDEI CTNAG 4 cut(s) 20, 144, 477, 708
BstF5I GGATG 6 cut(s) 10, 227, 487, 541, 577, 663
BstHHI GCGC 2 cut(s) 727, 907
BstKTI GATC 1 cut(s) 34
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 1 cut(s) 31
BstMCI CGRYCG 1 cut(s) 988
BstMWI GCNNNNNNNGC 4 cut(s) 140, 202, 614, 965
BstNI CCWGG 2 cut(s) 59, 690
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 2 cut(s) 57, 688
BstSFI CTRYAG 2 cut(s) 76, 669
BstV1I GCAGC 9 cut(s) 214, 232, 629, 655, 709, 828, 946, 949, 952
BstV2I GAAGAC 1 cut(s) 932
Bsu36I CCTNAGG 1 cut(s) 477
BsuRI GGCC 2 cut(s) 82, 704
BtsCI GGATG 6 cut(s) 10, 227, 487, 541, 577, 663
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 2 cut(s) 420, 495
Cac8I GCNNGC 3 cut(s) 606, 937, 970
CaiI CAGNNNCTG 1 cut(s) 1001
CciI TCATGA 1 cut(s) 510
CfoI GCGC 2 cut(s) 727, 907
Cfr13I GGNCC 1 cut(s) 703
Csp6I GTAC 3 cut(s) 176, 662, 884
CviAII CATG 3 cut(s) 368, 457, 511
CviQI GTAC 3 cut(s) 176, 662, 884
DdeI CTNAG 4 cut(s) 20, 144, 477, 708
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 441
Eco81I CCTNAGG 1 cut(s) 477
EcoO109I RGGNCCY 1 cut(s) 703
EcoRII CCWGG 2 cut(s) 57, 688
FaeI CATG 3 cut(s) 371, 460, 514
FaqI GGGAC 1 cut(s) 29
FatI CATG 3 cut(s) 367, 456, 510
FauI CCCGC 1 cut(s) 249
FokI GGATG 6 cut(s) 17, 214, 494, 548, 564, 670
FspBI CTAG 3 cut(s) 41, 396, 590
GlaI GCGC 2 cut(s) 726, 906
GsaI CCCAGC 1 cut(s) 320
HaeIII GGCC 2 cut(s) 82, 704
HhaI GCGC 2 cut(s) 727, 907
Hin1II CATG 3 cut(s) 371, 460, 514
Hin6I GCGC 2 cut(s) 725, 905
HinP1I GCGC 2 cut(s) 725, 905
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 3 cut(s) 146, 343, 392
HphI GGTGA 1 cut(s) 845
Hpy166II GTNNAC 2 cut(s) 16, 470
Hpy188I TCNGA 5 cut(s) 444, 624, 742, 783, 805
Hpy188III TCNNGA 5 cut(s) 35, 389, 511, 533, 996
Hpy8I GTNNAC 2 cut(s) 16, 470
HpyAV CCTTC 2 cut(s) 635, 892
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 8 cut(s) 367, 493, 671, 749, 844, 856, 880, 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 202, 614, 965
HpyF3I CTNAG 4 cut(s) 20, 144, 477, 708
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 3 cut(s) 371, 460, 514
HspAI GCGC 2 cut(s) 725, 905
Kzo9I GATC 1 cut(s) 31
LmnI GCTCC 1 cut(s) 998
Lsp1109I GCAGC 9 cut(s) 214, 232, 629, 655, 709, 828, 946, 949, 952
LweI GCATC 2 cut(s) 586, 736
MaeI CTAG 3 cut(s) 41, 396, 590
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 1 cut(s) 679
MalI GATC 1 cut(s) 33
MboI GATC 1 cut(s) 31
MboII GAAGA 6 cut(s) 368, 371, 428, 431, 800, 937
MfeI CAATTG 1 cut(s) 750
MluCI AATT 6 cut(s) 64, 237, 292, 750, 796, 887
MlyI GAGTC 1 cut(s) 155
MnlI CCTC 8 cut(s) 57, 241, 302, 399, 471, 805, 941, 983
MseI TTAA 3 cut(s) 96, 114, 297
MspA1I CMGCKG 3 cut(s) 143, 827, 935
MspR9I CCNGG 2 cut(s) 59, 690
MunI CAATTG 1 cut(s) 750
MvaI CCWGG 2 cut(s) 59, 690
MwoI GCNNNNNNNGC 4 cut(s) 140, 202, 614, 965
NdeII GATC 1 cut(s) 31
NlaIII CATG 3 cut(s) 371, 460, 514
NlaIV GGNNCC 2 cut(s) 705, 1006
NmuCI GTSAC 1 cut(s) 679
NspI RCATGY 1 cut(s) 371
PagI TCATGA 1 cut(s) 510
PfeI GAWTC 2 cut(s) 343, 392
PflFI GACNNNGTC 1 cut(s) 41
PfoI TCCNGGA 2 cut(s) 57, 688
PleI GAGTC 1 cut(s) 154
PpsI GAGTC 1 cut(s) 154
Psp6I CCWGG 2 cut(s) 57, 688
PspFI CCCAGC 1 cut(s) 316
PspGI CCWGG 2 cut(s) 57, 688
PspN4I GGNNCC 2 cut(s) 705, 1006
PspPI GGNCC 1 cut(s) 703
PsrI GAACNNNNNNTAC 2 cut(s) 937, 969
PstI CTGCAG 1 cut(s) 673
PstNI CAGNNNCTG 1 cut(s) 1001
PsyI GACNNNGTC 1 cut(s) 41
PvuII CAGCTG 2 cut(s) 827, 935
RsaI GTAC 3 cut(s) 177, 663, 885
RsaNI GTAC 3 cut(s) 176, 662, 884
SaqAI TTAA 3 cut(s) 96, 114, 297
Sau3AI GATC 1 cut(s) 31
Sau96I GGNCC 1 cut(s) 703
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 59, 690
SfaNI GCATC 2 cut(s) 586, 736
SfcI CTRYAG 2 cut(s) 76, 669
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 6 cut(s) 64, 237, 292, 750, 796, 887
SsiI CCGC 6 cut(s) 89, 141, 256, 587, 715, 984
SspMI CTAG 3 cut(s) 41, 396, 590
StyD4I CCNGG 2 cut(s) 57, 688
TaaI ACNGT 1 cut(s) 415
TaiI ACGT 1 cut(s) 121
TaqI TCGA 2 cut(s) 891, 988
TasI AATT 6 cut(s) 64, 237, 292, 750, 796, 887
TatI WGTACW 2 cut(s) 175, 883
TauI GCSGC 2 cut(s) 92, 143
TfiI GAWTC 2 cut(s) 343, 392
Tru1I TTAA 3 cut(s) 96, 114, 297
Tru9I TTAA 3 cut(s) 96, 114, 297
TscAI CASTG 2 cut(s) 420, 495
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 9 cut(s) 202, 245, 617, 668, 722, 841, 959, 962, 965
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 7 cut(s) 137, 216, 498, 499, 527, 710, 807
TspGWI ACGGA 1 cut(s) 455
TspRI CASTG 2 cut(s) 420, 495
Tth111I GACNNNGTC 1 cut(s) 41
XapI RAATTY 2 cut(s) 292, 796
XceI RCATGY 1 cut(s) 371
XspI CTAG 3 cut(s) 41, 396, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.