Rh5CG375100

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005505
Physical Location & Seq
Forward (+)
48473552 .. 48485965
12414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG375100.1

Sequence Viewer

Length: 2508 bp
ATGTCGCAGGCAGATATCAGAAAGTGGTTTATGAAATCGCATGACAAGGCCAATGCCTCCAAACCGGCTCCGGCTCCGGCTGCTTTGCCGAAACAGTCTACAGACCCCCCTCAAGAACAGAATGTGCATGGAGGTGAAGAAAATTCAGGCAGAAGGAAAACCAGCAAGTATTTTCCTCCAAAGCCGAATGAGGAAAAGGAAACAGTTGATGTTCCAGCAAAGCGGAAGACTCGACCGGATCCTGTTGAATCTAGCAGACCCTCGCCTGCAAAAAAAATTCAGAAAGTTGCTGATGACGACGACGACGACGACGATGATTTTGTTTTGCCTCAATCTAAAAAGAAAGAAGCCACGCCCAGCAAGAAACTGAAGAATGCATCTGGCATGGGAATTCCACAGAACCTGACGTCTGCAAATGAAAGTGATGATGATGAAGCCAAGGCTCCTCCCAAATCTGGTGGTAGGGGTCGTGGTGGAAGAGGTAGTTCATCGGCTGCACCAGCTGGTGGAAGGGGCAGAGGCGCTGGACGGGGTGGATTTATGAATTTTGGCGAAAGGAAAGATCCTCCACACAAAGGAGAAAAGGAGGTTCCTGAAGGTGATCCAAACTGTTTAGCTGGTTTAACTTTCGTAATAAGTGGTACACTTGATAGTTTGGAAAGAGAGGAGGCAGAAGATTTGATAAAACGCCATGGTGGTCGTATCACTGGATCTGTCAGCAAGAAAACGAGCTATCTTTTATGTGATGAAGATATTGAGGGCAGGAAATCCTCAAAAGCCAAAGAACTTGGGACGGCTTTCCTTACTGAGGATGGATTGTTTGATAAGATTCGGGCATCAATTCGTGCTAAAGCACCTGTGAAAGAAGAAAAGAAACCTGTGGATAGTGCACGAGAATCATCTCTGCCTAATAAAAGCCCTAAGAAAGTAGCATTAAAGAGTTCACCAGATTGCACTAGCAGCTCCTTGGCATGTAGGGTATCCCACAAACAGTCGCCATCAGATGCTTCCCTTGCTAGGCGCAAAGAGCAAACTACCGAGCATTCTGCTTTGACATGGACAGAAAAATATAGACCAAAGGTTCCAAATGACATTATTGGTAACCAGTCACTGGTAAAACAGCTTCATGATTGGTTGGCACATTGGCATGAGCAATTCCGTGATACTGGAGTTAAGAAAAAAGGCAATAAACCAAACAATACAGGTGCTAAAAAAGCTGTACTATTAAGTGGAACACCCGGAATAGGGAAAACAACGTCAGCAAAGTTGGTCAGTCAGATGCTCGGTTTCCAGGCAATTGAGGTAAATGCAAGTGACAGTCGTGGGAAAGCTGATTCCAAGATTGTCAAGGGAATCGGTGGAAGCAATGCAAACTCTGTTAAAGAGCTCGTCAGCAACGAGGCCCTAAGCATGGATAGATCTAAGCATCCCAAAACTGTGCTGATTATGGATGAGGTTGATGGGATGTCTGCTGGTGATCGGGGTGGAGTTGCTGATCTTATTGCTAGCATAAAGAGTTCTAAAATTCCCATTATCTGCATTTGTAATGATCGTTACAGTCAGAAACTGAAAAGTCTTGTGAACTACTGTTTGCTTCTCAGCTTCCGCAAACCCACTAAACAACAGATGGCAAAGAGGTTGAAGCAAGTTGCAAATGCTGAAGGACTTCAAGTGAATGAGATTGCTCTTGAGGAACTTGCAGAAAGAGTTCATGGAGACATGCGAATGGCACTTAACCAGTTGCACTATATGAGTCTCTCGATGTCTGTCATAAAATATGAAGATGTAAGGCAACGCCTACTAAGCAGTGCAAAGGATGAGGATATTTCACCATTCACAGCTGTTGACAAGCTGTTTGGTTTCAATGCTGGAAAGTTGCGGATGGATGAGCGGTTTGACCTGAGCATGAGTGATCCAGATCTAGTCCCTCTTCTTATTCAGGAAAATTATATTAACTACAGGCCAAGTGCGGCTGTGAAGGATGATAGTGGGGTTGAACGTATGAACTTGATTGCCCGTGCCGCTGAGTCTATTGGAAATGGGGATATTATCAATGTACAAATTAGAAAATATCGCCAATGGCAACTTTCCCAAAGTGGTTCTCTGTCATCCAGCATAATTCCAGCTGCTTTGTTGCGTGGGCAAAGGGAGACACTTGAGCAGGGAGAAAGAAATTTTAATAGATTTGGAGGTTGGCTAGGAAAGAACTCAACATTAGGCAAAAATCTGAGGCTCTTAGAAGATTTGCATTTCCATCTTCTTGCATCTCGTGAATCTAGTTCAGGGAGGGAGACCCTGCGAGTTGAATACCTTCCCCTACTTCTGAAACGATTGTCTGTGCCGCTGCGTAAATTGTTGAAGGATGAAGCTGTTCAGGAAGTTGTGGAGTTCATGAATACTTACTCCATTAGTCAAGATGACTATGATACTATTGTAGAGTTATCAAAATTTCAGGTAATCCTAACTAACTATATGGAGCGTGGTTATTGTAACAGTTCCAGAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

835

Amino Acids

91.98

Weight (kDa)

9.27

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT PF00533 202 - 275 4.5e-17 BRCA1 C Terminus (BRCT) domain
PTCB-BRCT PF12738 217 - 265 3.9e-06 twin BRCT domain
AAA_22 PF13401 404 - 494 3.7e-06 AAA domain
AAA PF00004 407 - 520 2.7e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_RFC1 PF25361 541 - 597 2.5e-08 RFC1-like, AAA+ ATPase lid domain
RCF1-5-like_lid PF21960 545 - 586 5.7e-06 RCF1/5-like, AAA+ ATPase lid domain
RFC1 PF08519 678 - 818 3.2e-33 Replication factor RFC1 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 410
AccB7I CCANNNNNTGG 2 cut(s) 506, 1111
AccBSI CCGCTC 1 cut(s) 1891
AccI GTMKAC 1 cut(s) 98
AciI CCGC 7 cut(s) 223, 1606, 1879, 1891, 1970, 2022, 2342
AclWI GGATC 6 cut(s) 233, 246, 557, 596, 718, 1907
AcsI RAATTY 8 cut(s) 142, 276, 390, 544, 1524, 2173, 2447, 2502
AcuI CTGAAG 3 cut(s) 389, 615, 1680
AcyI GRCGYC 1 cut(s) 407
AfaI GTAC 3 cut(s) 643, 1221, 2058
AfiI CCNNNNNNNGG 9 cut(s) 455, 506, 575, 808, 1017, 1111, 1244, 1245, 1411
AgsI TTSAA 7 cut(s) 248, 1642, 1670, 1864, 1997, 2306, 2359
AjnI CCWGG 1 cut(s) 1290
Alw21I GWGCWC 2 cut(s) 892, 1389
Alw26I GTCTC 4 cut(s) 1710, 1760, 2144, 2285
Alw44I GTGCAC 1 cut(s) 888
AlwI GGATC 6 cut(s) 233, 246, 557, 596, 718, 1907
AlwNI CAGNNNCTG 3 cut(s) 403, 1111, 1567
AoxI GGCC 3 cut(s) 48, 1401, 1961
ApaLI GTGCAC 1 cut(s) 888
ApeKI GCWGC 5 cut(s) 80, 494, 960, 2126, 2344
ApoI RAATTY 8 cut(s) 142, 276, 390, 544, 1524, 2173, 2447, 2502
ArsI GACNNNNNNTTYG 4 cut(s) 302, 334, 1838, 1870
Asp700I GAANNNNTTC 4 cut(s) 1665, 1707, 2310, 2370
AspLEI GCGC 2 cut(s) 524, 1023
AspS9I GGNCC 1 cut(s) 1402
AsuC2I CCSGG 1 cut(s) 1239
AsuHPI GGTGA 5 cut(s) 146, 611, 936, 1487, 1821
AsuNHI GCTAGC 1 cut(s) 1505
BaeGI GKGCMC 1 cut(s) 892
BaeI ACNNNNGTAYC 4 cut(s) 1155, 1188, 2418, 2451
BamHI GGATCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 1389
BarI GAAGNNNNNNTAC 2 cut(s) 1107, 1139
BauI CACGAG 2 cut(s) 891, 2268
BbsI GAAGAC 1 cut(s) 233
Bbv12I GWGCWC 2 cut(s) 892, 1389
BbvI GCAGC 5 cut(s) 67, 481, 972, 2113, 2331
BccI CCATC 6 cut(s) 806, 1006, 1454, 1621, 1876, 2262
BceAI ACGGC 1 cut(s) 810
BcgI CGANNNNNNTGC 2 cut(s) 1028, 1062
BciT130I CCWGG 1 cut(s) 1292
BciVI GTATCC 1 cut(s) 991
BcnI CCSGG 1 cut(s) 1239
BcoDI GTCTC 4 cut(s) 1710, 1760, 2144, 2285
BfaI CTAG 7 cut(s) 252, 957, 1017, 1506, 1922, 2198, 2277
BfmI CTRYAG 2 cut(s) 99, 1957
BfoI RGCGCY 1 cut(s) 525
BfuI GTATCC 1 cut(s) 991
BglII AGATCT 2 cut(s) 1418, 1918
BisI GCNGC 8 cut(s) 81, 495, 961, 1971, 2022, 2127, 2342, 2345
BlsI GCNGC 8 cut(s) 82, 496, 962, 1972, 2023, 2128, 2343, 2346
Bme1390I CCNGG 2 cut(s) 1239, 1292
BmgT120I GGNCC 1 cut(s) 1402
BmiI GGNNCC 6 cut(s) 69, 75, 240, 444, 591, 1083
BmrFI CCNGG 2 cut(s) 1239, 1292
BmsI GCATC 6 cut(s) 386, 845, 994, 1269, 1435, 2273
BmtI GCTAGC 1 cut(s) 1509
BpiI GAAGAC 1 cut(s) 233
BpmI CTGGAG 1 cut(s) 1188
Bpu10I CCTNAGC 2 cut(s) 1406, 1901
BpuEI CTTGAG 3 cut(s) 96, 1709, 2177
BpuMI CCSGG 1 cut(s) 1239
BsaBI GATNNNNATC 1 cut(s) 1917
BsaHI GRCGYC 1 cut(s) 407
BsaI GGTCTC 1 cut(s) 2285
BsaJI CCNNGG 3 cut(s) 438, 691, 966
BsaWI WCCGGW 1 cut(s) 235
Bsc4I CCNNNNNNNGG 9 cut(s) 455, 506, 575, 808, 1017, 1111, 1244, 1245, 1411
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 5 cut(s) 712, 1105, 1116, 1171, 1738
Bse3DI GCAATG 1 cut(s) 1372
Bse8I GATNNNNATC 1 cut(s) 1917
BseBI CCWGG 1 cut(s) 1292
BseDI CCNNGG 3 cut(s) 438, 691, 966
BseJI GATNNNNATC 1 cut(s) 1917
BseLI CCNNNNNNNGG 9 cut(s) 455, 506, 575, 808, 1017, 1111, 1244, 1245, 1411
BseMI GCAATG 1 cut(s) 1372
BseMII CTCAG 5 cut(s) 798, 1612, 1892, 2016, 2219
BseNI ACTGG 5 cut(s) 712, 1105, 1116, 1171, 1738
BseRI GAGGAG 2 cut(s) 435, 680
BseSI GKGCMC 1 cut(s) 892
BseXI GCAGC 5 cut(s) 67, 481, 972, 2113, 2331
BseYI CCCAGC 1 cut(s) 356
BsgI GTGCAG 1 cut(s) 480
Bsh1285I CGRYCG 1 cut(s) 236
BshFI GGCC 3 cut(s) 50, 1403, 1963
BsiEI CGRYCG 1 cut(s) 236
BsiHKAI GWGCWC 2 cut(s) 892, 1389
BsiSI CCGG 5 cut(s) 65, 71, 77, 236, 1239
BslFI GGGAC 2 cut(s) 805, 1910
BslI CCNNNNNNNGG 9 cut(s) 455, 506, 575, 808, 1017, 1111, 1244, 1245, 1411
BsmAI GTCTC 4 cut(s) 1710, 1760, 2144, 2285
BsmFI GGGAC 2 cut(s) 805, 1910
BsmI GAATGC 2 cut(s) 379, 1042
BsnI GGCC 3 cut(s) 50, 1403, 1963
Bso31I GGTCTC 1 cut(s) 2285
Bsp1286I GDGCHC 2 cut(s) 892, 1389
Bsp1407I TGTACA 1 cut(s) 2056
Bsp19I CCATGG 1 cut(s) 691
BspACI CCGC 7 cut(s) 223, 1606, 1879, 1891, 1970, 2022, 2342
BspANI GGCC 3 cut(s) 50, 1403, 1963
BspCNI CTCAG 5 cut(s) 799, 1611, 1893, 2017, 2220
BspHI TCATGA 2 cut(s) 1126, 2391
BspLI GGNNCC 6 cut(s) 69, 75, 240, 444, 591, 1083
BspOI GCTAGC 1 cut(s) 1509
BspPI GGATC 6 cut(s) 233, 246, 557, 596, 718, 1907
BspTNI GGTCTC 1 cut(s) 2285
BsrBI CCGCTC 1 cut(s) 1891
BsrDI GCAATG 1 cut(s) 1372
BsrFI RCCGGY 1 cut(s) 64
BsrGI TGTACA 1 cut(s) 2056
BsrI ACTGG 5 cut(s) 712, 1105, 1116, 1171, 1738
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 3 cut(s) 438, 691, 966
BssNI GRCGYC 1 cut(s) 407
BssSI CACGAG 2 cut(s) 891, 2268
BssT1I CCWWGG 3 cut(s) 438, 691, 966
Bst2BI CACGAG 2 cut(s) 891, 2268
Bst2UI CCWGG 1 cut(s) 1292
Bst4CI ACNGT 9 cut(s) 96, 205, 611, 993, 1319, 1438, 1559, 1589, 2495
Bst6I CTCTTC 2 cut(s) 472, 1935
BstACI GRCGYC 1 cut(s) 407
BstAUI TGTACA 1 cut(s) 2056
BstC8I GCNNGC 3 cut(s) 9, 267, 1507
BstDSI CCRYGG 1 cut(s) 691
BstEII GGTNACC 1 cut(s) 1100
BstENI CCTNNNNNAGG 1 cut(s) 806
BstH2I RGCGCY 1 cut(s) 525
BstHHI GCGC 2 cut(s) 524, 1023
BstMAI GTCTC 4 cut(s) 1710, 1760, 2144, 2285
BstMCI CGRYCG 1 cut(s) 236
BstMWI GCNNNNNNNGC 8 cut(s) 80, 500, 960, 1013, 1027, 1214, 1803, 2021
BstNI CCWGG 1 cut(s) 1292
BstNSI RCATGY 2 cut(s) 975, 1723
BstPI GGTNACC 1 cut(s) 1100
BstSCI CCNGG 2 cut(s) 1237, 1290
BstSFI CTRYAG 2 cut(s) 99, 1957
BstSLI GKGCMC 1 cut(s) 892
BstV1I GCAGC 5 cut(s) 67, 481, 972, 2113, 2331
BstV2I GAAGAC 1 cut(s) 233
BstX2I RGATCY 5 cut(s) 238, 562, 710, 1418, 1918
BstYI RGATCY 5 cut(s) 238, 562, 710, 1418, 1918
BsuI GTATCC 1 cut(s) 991
BsuRI GGCC 3 cut(s) 50, 1403, 1963
BtgI CCRYGG 1 cut(s) 691
BtsI GCAGTG 1 cut(s) 1813
BtsIMutI CAGTG 3 cut(s) 705, 1109, 1813
Cac8I GCNNGC 3 cut(s) 9, 267, 1507
CaiI CAGNNNCTG 3 cut(s) 403, 1111, 1567
CciI TCATGA 2 cut(s) 1126, 2391
CfoI GCGC 2 cut(s) 524, 1023
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 1 cut(s) 1402
Csp6I GTAC 3 cut(s) 642, 1220, 2057
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviQI GTAC 3 cut(s) 642, 1220, 2057
Eam1104I CTCTTC 2 cut(s) 472, 1935
EarI CTCTTC 2 cut(s) 472, 1935
Ecl136II GAGCTC 1 cut(s) 1387
Eco130I CCWWGG 3 cut(s) 438, 691, 966
Eco24I GRGCYC 1 cut(s) 1389
Eco31I GGTCTC 1 cut(s) 2285
Eco32I GATATC 1 cut(s) 16
Eco53kI GAGCTC 1 cut(s) 1387
Eco57I CTGAAG 3 cut(s) 389, 615, 1680
Eco91I GGTNACC 1 cut(s) 1100
EcoICRI GAGCTC 1 cut(s) 1387
EcoNI CCTNNNNNAGG 1 cut(s) 806
EcoO109I RGGNCCY 1 cut(s) 1402
EcoO65I GGTNACC 1 cut(s) 1100
EcoRI GAATTC 1 cut(s) 390
EcoRII CCWGG 1 cut(s) 1290
EcoRV GATATC 1 cut(s) 16
EcoT14I CCWWGG 3 cut(s) 438, 691, 966
EcoT22I ATGCAT 1 cut(s) 379
EcoT38I GRGCYC 1 cut(s) 1389
ErhI CCWWGG 3 cut(s) 438, 691, 966
FaqI GGGAC 2 cut(s) 805, 1910
FblI GTMKAC 1 cut(s) 98
Fnu4HI GCNGC 8 cut(s) 81, 495, 961, 1971, 2022, 2127, 2342, 2345
FriOI GRGCYC 1 cut(s) 1389
Fsp4HI GCNGC 8 cut(s) 81, 495, 961, 1971, 2022, 2127, 2342, 2345
FspBI CTAG 7 cut(s) 252, 957, 1017, 1506, 1922, 2198, 2277
GlaI GCGC 2 cut(s) 523, 1022
GluI GCNGC 8 cut(s) 81, 495, 961, 1971, 2022, 2127, 2342, 2345
GsaI CCCAGC 1 cut(s) 360
GsuI CTGGAG 1 cut(s) 1188
HaeII RGCGCY 1 cut(s) 525
HaeIII GGCC 3 cut(s) 50, 1403, 1963
HapII CCGG 5 cut(s) 65, 71, 77, 236, 1239
HhaI GCGC 2 cut(s) 524, 1023
Hin1I GRCGYC 1 cut(s) 407
Hin6I GCGC 2 cut(s) 522, 1021
HinP1I GCGC 2 cut(s) 522, 1021
HincII GTYRAC 1 cut(s) 1846
HindII GTYRAC 1 cut(s) 1846
HinfI GANTC 9 cut(s) 229, 248, 829, 896, 1334, 1353, 1753, 2027, 2273
HpaII CCGG 5 cut(s) 65, 71, 77, 236, 1239
HphI GGTGA 5 cut(s) 146, 611, 936, 1487, 1821
Hpy166II GTNNAC 6 cut(s) 99, 644, 890, 944, 1582, 1846
Hpy188I TCNGA 7 cut(s) 20, 282, 1003, 1278, 1563, 2229, 2325
Hpy8I GTNNAC 6 cut(s) 99, 644, 890, 944, 1582, 1846
Hpy99I CGWCG 5 cut(s) 302, 305, 308, 311, 314
HpyAV CCTTC 7 cut(s) 147, 504, 590, 1655, 1972, 2321, 2353
HpyCH4III ACNGT 9 cut(s) 96, 205, 611, 993, 1319, 1438, 1559, 1589, 2495
HpyCH4IV ACGT 3 cut(s) 407, 1256, 1999
HpyF10VI GCNNNNNNNGC 8 cut(s) 80, 500, 960, 1013, 1027, 1214, 1803, 2021
HpySE526I ACGT 3 cut(s) 407, 1256, 1999
Hsp92I GRCGYC 1 cut(s) 407
HspAI GCGC 2 cut(s) 522, 1021
LmnI GCTCC 5 cut(s) 73, 79, 448, 968, 2476
Lsp1109I GCAGC 5 cut(s) 67, 481, 972, 2113, 2331
LweI GCATC 6 cut(s) 386, 845, 994, 1269, 1435, 2273
MaeI CTAG 7 cut(s) 252, 957, 1017, 1506, 1922, 2198, 2277
MaeII ACGT 3 cut(s) 407, 1256, 1999
MaeIII GTNAC 5 cut(s) 1100, 1107, 1313, 1553, 2489
MbiI CCGCTC 1 cut(s) 1891
MfeI CAATTG 1 cut(s) 1296
MflI RGATCY 5 cut(s) 238, 562, 710, 1418, 1918
MhlI GDGCHC 2 cut(s) 892, 1389
MlyI GAGTC 3 cut(s) 223, 1762, 2036
Mph1103I ATGCAT 1 cut(s) 379
MroXI GAANNNNTTC 4 cut(s) 1665, 1707, 2310, 2370
MseI TTAA 8 cut(s) 623, 935, 1173, 1226, 1380, 1734, 1953, 2178
MslI CAYNNNNRTG 3 cut(s) 132, 1146, 1724
MspA1I CMGCKG 5 cut(s) 503, 1841, 2024, 2126, 2344
MspI CCGG 5 cut(s) 65, 71, 77, 236, 1239
MspR9I CCNGG 2 cut(s) 1239, 1292
MunI CAATTG 1 cut(s) 1296
Mva1269I GAATGC 2 cut(s) 379, 1042
MvaI CCWGG 1 cut(s) 1292
MwoI GCNNNNNNNGC 8 cut(s) 80, 500, 960, 1013, 1027, 1214, 1803, 2021
NciI CCSGG 1 cut(s) 1239
NcoI CCATGG 1 cut(s) 691
NheI GCTAGC 1 cut(s) 1505
NlaIV GGNNCC 6 cut(s) 69, 75, 240, 444, 591, 1083
NmuCI GTSAC 2 cut(s) 1107, 1313
NsiI ATGCAT 1 cut(s) 379
NspI RCATGY 2 cut(s) 975, 1723
PagI TCATGA 2 cut(s) 1126, 2391
PcsI WCGNNNNNNNCGW 4 cut(s) 303, 306, 309, 1395
PctI GAATGC 2 cut(s) 379, 1042
PdmI GAANNNNTTC 4 cut(s) 1665, 1707, 2310, 2370
PfeI GAWTC 6 cut(s) 248, 829, 896, 1334, 1353, 2273
PflMI CCANNNNNTGG 2 cut(s) 506, 1111
PkrI GCNGC 8 cut(s) 82, 496, 962, 1972, 2023, 2128, 2343, 2346
PleI GAGTC 3 cut(s) 223, 1761, 2035
PpsI GAGTC 3 cut(s) 223, 1761, 2035
Psp124BI GAGCTC 1 cut(s) 1389
Psp6I CCWGG 1 cut(s) 1290
PspEI GGTNACC 1 cut(s) 1100
PspFI CCCAGC 1 cut(s) 356
PspGI CCWGG 1 cut(s) 1290
PspN4I GGNNCC 6 cut(s) 69, 75, 240, 444, 591, 1083
PspPI GGNCC 1 cut(s) 1402
PstNI CAGNNNCTG 3 cut(s) 403, 1111, 1567
PsuI RGATCY 5 cut(s) 238, 562, 710, 1418, 1918
PvuII CAGCTG 3 cut(s) 503, 1841, 2126
RsaI GTAC 3 cut(s) 643, 1221, 2058
RsaNI GTAC 3 cut(s) 642, 1220, 2057
RseI CAYNNNNRTG 3 cut(s) 132, 1146, 1724
SacI GAGCTC 1 cut(s) 1389
SaqAI TTAA 8 cut(s) 623, 935, 1173, 1226, 1380, 1734, 1953, 2178
SatI GCNGC 8 cut(s) 81, 495, 961, 1971, 2022, 2127, 2342, 2345
Sau96I GGNCC 1 cut(s) 1402
SchI GAGTC 3 cut(s) 223, 1762, 2036
ScrFI CCNGG 2 cut(s) 1239, 1292
SduI GDGCHC 2 cut(s) 892, 1389
SfaNI GCATC 6 cut(s) 386, 845, 994, 1269, 1435, 2273
SfcI CTRYAG 2 cut(s) 99, 1957
SmiMI CAYNNNNRTG 3 cut(s) 132, 1146, 1724
SmlI CTYRAG 3 cut(s) 111, 1688, 2156
SmoI CTYRAG 3 cut(s) 111, 1688, 2156
SsiI CCGC 7 cut(s) 223, 1606, 1879, 1891, 1970, 2022, 2342
SspMI CTAG 7 cut(s) 252, 957, 1017, 1506, 1922, 2198, 2277
SstI GAGCTC 1 cut(s) 1389
StyD4I CCNGG 2 cut(s) 1237, 1290
StyI CCWWGG 3 cut(s) 438, 691, 966
TaaI ACNGT 9 cut(s) 96, 205, 611, 993, 1319, 1438, 1559, 1589, 2495
TaiI ACGT 3 cut(s) 410, 1259, 2002
TaqI TCGA 2 cut(s) 232, 1760
TatI WGTACW 2 cut(s) 1219, 2056
TauI GCSGC 3 cut(s) 1973, 2024, 2344
TfiI GAWTC 6 cut(s) 248, 829, 896, 1334, 1353, 2273
Tru1I TTAA 8 cut(s) 623, 935, 1173, 1226, 1380, 1734, 1953, 2178
Tru9I TTAA 8 cut(s) 623, 935, 1173, 1226, 1380, 1734, 1953, 2178
TscAI CASTG 3 cut(s) 712, 1116, 1813
TseFI GTSAC 2 cut(s) 1107, 1313
TseI GCWGC 5 cut(s) 80, 494, 960, 2126, 2344
Tsp45I GTSAC 2 cut(s) 1107, 1313
TspGWI ACGGA 1 cut(s) 1148
TspRI CASTG 3 cut(s) 712, 1116, 1813
Van91I CCANNNNNTGG 2 cut(s) 506, 1111
VneI GTGCAC 1 cut(s) 888
XagI CCTNNNNNAGG 1 cut(s) 806
XapI RAATTY 8 cut(s) 142, 276, 390, 544, 1524, 2173, 2447, 2502
XceI RCATGY 2 cut(s) 975, 1723
XmiI GTMKAC 1 cut(s) 98
XmnI GAANNNNTTC 4 cut(s) 1665, 1707, 2310, 2370
XspI CTAG 7 cut(s) 252, 957, 1017, 1506, 1922, 2198, 2277
ZraI GACGTC 1 cut(s) 408
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.