RLG00000022682

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005505
Physical Location & Seq
Reverse (-)
9399740 .. 9420645
20906 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022682

Sequence Viewer

Length: 2784 bp
ATGGTTGTTCTGTATGCCTCAACAGTGAGCATTAGCGTTTCGCATTCTGCAACCCAACCCAAGCTAACAATGCCGCAGGCAGATATCAGAAAGTGGTTTATGAAATCGCATGACAAGGGCAATGCCTCCAAACCGGCACCAGCTCCGGTTCCGGCTGCTTTGCAGAAACAGTCTACAGACCCTCAAGAACAGAATGTGCATGGAGGTGAAGAGAATTCAGGCGGAAGGAAAATTAGCAAGTATTTTCCTTCAAATAAACCGAAGCCGAATGAGGAAAAGGAAACAGTTGATAAACTGAAGAATGCATCTGGCATGGGAATTCCACAGAACCTGACGTCTGCAAATGAAAGTGATGATGATGATGCCAAGGCTCCTCGCAGATCTGGTGGTAGGGGTCATGGTGGAAGAGGTAGTTCATCGGCCGCACCAGCTGGTGGAAGAGGTAGAGGCGCTGGACGGGGTGGGTTTATGAATTTTGGCGAAAGGAAAGATCCTCCACACAAAGGAGACAAGGAGATTCCTGAAGGTGATCCAAACTGTTTAGCTGGTTTAACTTTCGTAATAAGTGGTACACTTGATAGTTTGGAAAGAGAGGAGGCAGAAGATTTGATAAAACGCCATGGTGGTTCAGCCTGTTTGCAGAGCTATCTTTTATGTGATGAAGATATTGGGGGCAGGAAATCCTCAAAAGCCAAAGAACTTGGGACGGCTTTCCTTACTGAGGATGGCTTGTTTGATAAGATTCAGGCATCAATTCGTGCTAAAGCACCTCTGAAAGAAGAAAAGAAACCTCTGGATAGTGCAAGAGAAGCATCTTTGCCTAATAAAAGCCCTAAGAAAGAAGCATTCAAGAGTTCACCAGAATGCACTAGCTGCTCCTTGGCGTGTAGGGTATCCCACAAACAGTTGCCATCAGATGCTTTGCTTGCTATGTGCAGAGAGCAAACTGCTGAGCGTCCTGCTTTGACATGGACGGAAAAATATAGACCAAAGGTTCCAAATGACATTATTGGTAACCGGTCACTGGTAAAGCAGCTTCATGGTTGGTTGGCACATTGGCATGAGCAATTCCACGATACTGCAGTTAAGAAAAAAGGCAATAAACCAAACAATTCAGGTGCTAAAAAAGCTGTACTATTAAGTGGAACACCTGGAATTGGAAAAACAACGTCAGCAAAGTTGGTAAATGCAAGTGACAGTCGTGGGAAAGCTGATTCCAAGATTGTCAAGGGAATCGATGGAAGCAATGCAAACTCTGTTAAAGAACTCGTCAACAACGAGGCCCTAAGCATGGATAGATCTAAGCATCCCAAAACTGTGCTGATTATGGATGAGGTTGATGGAATGTCTGCTGGTGATCAGGGTGGAGTTGCTGATCTTATTGCTAGCATAAAGAGTTCTAAAATTCCCATTATCTGCATTTGTAATGATCAATACATTCAGAAACTGAAAAGTCTTGTGAAATACTGTTTGCTTCTCAGCTTCCGCAAACCCAGTAAACAACAGATGGCAAAGAGGTTGAAGCAAGTTGCAAATTTTGAAGGACTTCAAGTGAATGAGATTGCTCTTGAGGAACTTGCAGAAAGAGTTAACGGAGACATGCGAATGGCACTTAACCAGTTGCAGTATATGGGTCTCTTGATGTCTGTCATAAAATATGAAGATGTAAGGCAACGCCTACTAAGCAGTGCAAGGGATGAGGATATTTCACCATTCACAGCTGTTGACAAGCTGTTTGGTTTCAATGCTGGAAAGTTGCGGATGGATGAGCGGTTTGACCTGAGCATGAGTGATCCAGATCTAGTCCCTCTTCTTATTCAGGAAAATTATTTTAACTACAGGCCAAGTGCGGTTGTGAAGGATGATAGTGGGGTTGAATGTATGAACTTGATTGCCGGTGCCACTGAGTCTATTGGAAATGGAGATATTATCAATGTACAAATTAGAAAATATCGCCAATGGCAACTTTCCCAAAGTCGTTCTCTGTCATCCAGCATAATTCCTGCTGCTTTGTTGCGTGGGCAAAGGGAGACACTTGAGCTGGGAGAAAGAAATTTTAATAGATTTGGAGGTTGGCTGGGAAAGAACTCGACATTAGGCAAAAATCTGAGGCTCTTAGAAGATTTGCATTTCCATCTTCTTGCATCTCGTGAATCTAGTTCAGGGAGGGAGACCCTGCGAGTTGAATACCTTCCCCTACTTCTGAAACGATTGTCTGTGCCGCTGCGTAAATTGTCGAAGGATGAAGCTGTTCAGGAAGTTGTGGAGTTCATGAATACTTACTCCATTAGTCAGGATGACTATGATACTATCGTAGAGTTATCAAAATTTCAGGAGCATCCAAATCCGCTGGATGGTGTCCAGCCGGCTGTAAAAGCGGCTCTGACAAAAGCATACAAAGAAGGAAGCAAATCAAGGATGGTACGAGTTGCAGAGCTTGTCACTTTCCCTGGAATGAAAAAGGCTCCTAAGAAGAGGATTGCAGCTATTTTAGAACCATCTGACGATGTAATTGGAGAGAACAATGATGATGCAGAGGTTCTAAGTGAAGAAGAAAATTCATCTGAGACAGAGGATTTGGAAAGCTCTGCTGTTGGGGAGAAGCTGCAAGAGGAACTTCAAAGTTTGAATAAAAAAGGAGTGCAAGTTCAATTGGATTTGAAGGGTGCAGCAAATCCAAGTGCCAAGAGGACACCAGCTGGAAGAGGTAAAGGTGGTTCTTCTGGTGCCTCTGAGAAAAAAGGTGGCCGAGGTTCAGGGACTGGTTCCAAAAGAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

928

Amino Acids

101.13

Weight (kDa)

9.08

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT PF00533 178 - 246 2.3e-08 BRCA1 C Terminus (BRCT) domain
AAA PF00004 378 - 484 1.1e-06 ATPase family associated with various cellular activities (AAA)
AAA_lid_RFC1 PF25361 501 - 557 2.3e-06 RFC1-like, AAA+ ATPase lid domain
RFC1 PF08519 638 - 800 1.5e-40 Replication factor RFC1 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 338
AccB1I GGYRCC 3 cut(s) 136, 1898, 2726
AccB7I CCANNNNNTGG 1 cut(s) 434
AccBSI CCGCTC 1 cut(s) 1771
AccI GTMKAC 1 cut(s) 173
AclWI GGATC 3 cut(s) 485, 524, 1787
AcoI YGGCCR 2 cut(s) 420, 2746
AcsI RAATTY 8 cut(s) 214, 318, 472, 1404, 1534, 2053, 2327, 2557
AcuI CTGAAG 2 cut(s) 317, 543
AcyI GRCGYC 1 cut(s) 335
AfaI GTAC 4 cut(s) 571, 1134, 1938, 2424
AfiI CCNNNNNNNGG 7 cut(s) 434, 503, 721, 1024, 1157, 1291, 2354
AgeI ACCGGT 1 cut(s) 1017
AjnI CCWGG 2 cut(s) 1150, 2449
AloI GAACNNNNNNTCC 2 cut(s) 2631, 2663
Alw26I GTCTC 6 cut(s) 501, 1590, 1640, 2024, 2165, 2561
AlwI GGATC 3 cut(s) 485, 524, 1787
AlwNI CAGNNNCTG 3 cut(s) 331, 1447, 2762
AoxI GGCC 4 cut(s) 420, 1281, 1841, 2746
ApeKI GCWGC 8 cut(s) 155, 873, 1033, 2006, 2224, 2483, 2605, 2669
ApoI RAATTY 8 cut(s) 214, 318, 472, 1404, 1534, 2053, 2327, 2557
ArsI GACNNNNNNTTYG 4 cut(s) 1718, 1750, 2560, 2592
AsiGI ACCGGT 1 cut(s) 1017
Asp700I GAANNNNTTC 3 cut(s) 1545, 2190, 2250
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 1 cut(s) 1282
AsuHPI GGTGA 5 cut(s) 218, 539, 849, 1367, 1701
AsuNHI GCTAGC 1 cut(s) 1385
BaeI ACNNNNGTAYC 4 cut(s) 1068, 1101, 2298, 2331
BanI GGYRCC 3 cut(s) 136, 1898, 2726
BarI GAAGNNNNNNTAC 2 cut(s) 1020, 1052
BauI CACGAG 1 cut(s) 2148
BbvI GCAGC 8 cut(s) 142, 860, 1045, 1993, 2211, 2495, 2592, 2681
BceAI ACGGC 1 cut(s) 723
BciT130I CCWGG 2 cut(s) 1152, 2451
BciVI GTATCC 1 cut(s) 904
BclI TGATCA 2 cut(s) 1357, 1429
BcoDI GTCTC 6 cut(s) 501, 1590, 1640, 2024, 2165, 2561
BfaI CTAG 4 cut(s) 870, 1386, 1802, 2157
BfmI CTRYAG 3 cut(s) 174, 1080, 1837
BfoI RGCGCY 1 cut(s) 453
BfuI GTATCC 1 cut(s) 904
BglII AGATCT 3 cut(s) 380, 1298, 1798
BlpI GCTNAGC 1 cut(s) 951
Bme1390I CCNGG 2 cut(s) 1152, 2451
BmgT120I GGNCC 1 cut(s) 1282
BmiI GGNNCC 8 cut(s) 138, 150, 372, 996, 1900, 2466, 2728, 2767
BmrFI CCNGG 2 cut(s) 1152, 2451
BmrI ACTGGG 1 cut(s) 1488
BmsI GCATC 9 cut(s) 314, 352, 758, 821, 907, 1315, 2153, 2347, 2521
BmtI GCTAGC 1 cut(s) 1389
BmuI ACTGGG 1 cut(s) 1488
Bpu10I CCTNAGC 2 cut(s) 1286, 1781
Bpu1102I GCTNAGC 1 cut(s) 951
BpuEI CTTGAG 3 cut(s) 168, 1589, 2057
Bsa29I ATCGAT 1 cut(s) 1236
BsaBI GATNNNNATC 1 cut(s) 1797
BsaHI GRCGYC 1 cut(s) 335
BsaI GGTCTC 2 cut(s) 1640, 2165
BsaJI CCNNGG 5 cut(s) 366, 619, 879, 2449, 2749
BsaWI WCCGGW 2 cut(s) 145, 1017
BsaXI ACNNNNNCTCC 2 cut(s) 2631, 2661
Bsc4I CCNNNNNNNGG 7 cut(s) 434, 503, 721, 1024, 1157, 1291, 2354
Bse118I RCCGGY 4 cut(s) 133, 1017, 1895, 2365
Bse1I ACTGG 4 cut(s) 1029, 1494, 1618, 2767
Bse3DI GCAATG 2 cut(s) 127, 1252
Bse8I GATNNNNATC 1 cut(s) 1797
BseBI CCWGG 2 cut(s) 1152, 2451
BseCI ATCGAT 1 cut(s) 1236
BseDI CCNNGG 5 cut(s) 366, 619, 879, 2449, 2749
BseJI GATNNNNATC 1 cut(s) 1797
BseLI CCNNNNNNNGG 7 cut(s) 434, 503, 721, 1024, 1157, 1291, 2354
BseMI GCAATG 2 cut(s) 127, 1252
BseMII CTCAG 8 cut(s) 711, 942, 1492, 1772, 1896, 2099, 2556, 2724
BseNI ACTGG 4 cut(s) 1029, 1494, 1618, 2767
BseRI GAGGAG 2 cut(s) 363, 608
BseX3I CGGCCG 1 cut(s) 420
BseXI GCAGC 8 cut(s) 142, 860, 1045, 1993, 2211, 2495, 2592, 2681
BseYI CCCAGC 2 cut(s) 2041, 2077
BsgI GTGCAG 2 cut(s) 955, 2688
Bsh1285I CGRYCG 1 cut(s) 423
BshFI GGCC 4 cut(s) 422, 1283, 1843, 2748
BshNI GGYRCC 3 cut(s) 136, 1898, 2726
BshTI ACCGGT 1 cut(s) 1017
BshVI ATCGAT 1 cut(s) 1236
BsiEI CGRYCG 1 cut(s) 423
BsiSI CCGG 6 cut(s) 134, 146, 152, 1018, 1896, 2366
BslFI GGGAC 3 cut(s) 718, 1790, 2773
BslI CCNNNNNNNGG 7 cut(s) 434, 503, 721, 1024, 1157, 1291, 2354
BsmAI GTCTC 6 cut(s) 501, 1590, 1640, 2024, 2165, 2561
BsmFI GGGAC 3 cut(s) 718, 1790, 2773
BsmI GAATGC 4 cut(s) 43, 307, 845, 869
BsnI GGCC 4 cut(s) 422, 1283, 1843, 2748
Bso31I GGTCTC 2 cut(s) 1640, 2165
Bsp1407I TGTACA 1 cut(s) 1936
Bsp143I GATC 9 cut(s) 380, 490, 529, 1298, 1357, 1375, 1429, 1792, 1798
Bsp1720I GCTNAGC 1 cut(s) 951
Bsp19I CCATGG 1 cut(s) 619
BspANI GGCC 4 cut(s) 422, 1283, 1843, 2748
BspCNI CTCAG 8 cut(s) 712, 943, 1491, 1773, 1897, 2100, 2557, 2725
BspDI ATCGAT 1 cut(s) 1236
BspHI TCATGA 1 cut(s) 2271
BspLI GGNNCC 8 cut(s) 138, 150, 372, 996, 1900, 2466, 2728, 2767
BspMAI CTGCAG 1 cut(s) 1084
BspOI GCTAGC 1 cut(s) 1389
BspPI GGATC 3 cut(s) 485, 524, 1787
BspT107I GGYRCC 3 cut(s) 136, 1898, 2726
BspTNI GGTCTC 2 cut(s) 1640, 2165
BsrBI CCGCTC 1 cut(s) 1771
BsrDI GCAATG 2 cut(s) 127, 1252
BsrFI RCCGGY 4 cut(s) 133, 1017, 1895, 2365
BsrGI TGTACA 1 cut(s) 1936
BsrI ACTGG 4 cut(s) 1029, 1494, 1618, 2767
BssAI RCCGGY 4 cut(s) 133, 1017, 1895, 2365
BssECI CCNNGG 5 cut(s) 366, 619, 879, 2449, 2749
BssMI GATC 9 cut(s) 380, 490, 529, 1298, 1357, 1375, 1429, 1792, 1798
BssNI GRCGYC 1 cut(s) 335
BssSI CACGAG 1 cut(s) 2148
BssT1I CCWWGG 3 cut(s) 366, 619, 879
Bst2BI CACGAG 1 cut(s) 2148
Bst2UI CCWGG 2 cut(s) 1152, 2451
Bst4CI ACNGT 8 cut(s) 25, 171, 286, 539, 906, 1199, 1318, 1469
Bst6I CTCTTC 6 cut(s) 204, 400, 433, 1815, 2468, 2698
BstACI GRCGYC 1 cut(s) 335
BstAPI GCANNNNNTGC 1 cut(s) 873
BstAUI TGTACA 1 cut(s) 1936
BstC8I GCNNGC 4 cut(s) 78, 927, 1387, 2367
BstDSI CCRYGG 1 cut(s) 619
BstEII GGTNACC 1 cut(s) 1013
BstENI CCTNNNNNAGG 1 cut(s) 719
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 9 cut(s) 383, 493, 532, 1301, 1360, 1378, 1432, 1795, 1801
BstMAI GTCTC 6 cut(s) 501, 1590, 1640, 2024, 2165, 2561
BstMBI GATC 9 cut(s) 380, 490, 529, 1298, 1357, 1375, 1429, 1792, 1798
BstMCI CGRYCG 1 cut(s) 423
BstMWI GCNNNNNNNGC 8 cut(s) 70, 428, 809, 873, 926, 1127, 1683, 2375
BstNI CCWGG 2 cut(s) 1152, 2451
BstNSI RCATGY 1 cut(s) 1603
BstPI GGTNACC 1 cut(s) 1013
BstSCI CCNGG 2 cut(s) 1150, 2449
BstSFI CTRYAG 3 cut(s) 174, 1080, 1837
BstV1I GCAGC 8 cut(s) 142, 860, 1045, 1993, 2211, 2495, 2592, 2681
BstX2I RGATCY 4 cut(s) 380, 490, 1298, 1798
BstYI RGATCY 4 cut(s) 380, 490, 1298, 1798
BstZI CGGCCG 1 cut(s) 420
Bsu15I ATCGAT 1 cut(s) 1236
BsuI GTATCC 1 cut(s) 904
BsuRI GGCC 4 cut(s) 422, 1283, 1843, 2748
BsuTUI ATCGAT 1 cut(s) 1236
BtgI CCRYGG 1 cut(s) 619
BtsI GCAGTG 1 cut(s) 1693
BtsIMutI CAGTG 4 cut(s) 30, 1022, 1693, 1902
Cac8I GCNNGC 4 cut(s) 78, 927, 1387, 2367
CaiI CAGNNNCTG 3 cut(s) 331, 1447, 2762
CciI TCATGA 1 cut(s) 2271
CfoI GCGC 1 cut(s) 452
Cfr10I RCCGGY 4 cut(s) 133, 1017, 1895, 2365
Cfr13I GGNCC 1 cut(s) 1282
ClaI ATCGAT 1 cut(s) 1236
CseI GACGC 1 cut(s) 944
Csp6I GTAC 4 cut(s) 570, 1133, 1937, 2423
CspAI ACCGGT 1 cut(s) 1017
CviQI GTAC 4 cut(s) 570, 1133, 1937, 2423
DpnI GATC 9 cut(s) 382, 492, 531, 1300, 1359, 1377, 1431, 1794, 1800
DpnII GATC 9 cut(s) 380, 490, 529, 1298, 1357, 1375, 1429, 1792, 1798
EaeI YGGCCR 2 cut(s) 420, 2746
EagI CGGCCG 1 cut(s) 420
Eam1104I CTCTTC 6 cut(s) 204, 400, 433, 1815, 2468, 2698
EarI CTCTTC 6 cut(s) 204, 400, 433, 1815, 2468, 2698
EciI GGCGGA 1 cut(s) 237
EclXI CGGCCG 1 cut(s) 420
Eco130I CCWWGG 3 cut(s) 366, 619, 879
Eco31I GGTCTC 2 cut(s) 1640, 2165
Eco32I GATATC 1 cut(s) 85
Eco52I CGGCCG 1 cut(s) 420
Eco57I CTGAAG 2 cut(s) 317, 543
Eco91I GGTNACC 1 cut(s) 1013
EcoNI CCTNNNNNAGG 1 cut(s) 719
EcoO109I RGGNCCY 1 cut(s) 1282
EcoO65I GGTNACC 1 cut(s) 1013
EcoRI GAATTC 2 cut(s) 214, 318
EcoRII CCWGG 2 cut(s) 1150, 2449
EcoRV GATATC 1 cut(s) 85
EcoT14I CCWWGG 3 cut(s) 366, 619, 879
EcoT22I ATGCAT 1 cut(s) 307
ErhI CCWWGG 3 cut(s) 366, 619, 879
FalI AAGNNNNNCTT 2 cut(s) 2601, 2633
FaqI GGGAC 3 cut(s) 718, 1790, 2773
FbaI TGATCA 2 cut(s) 1357, 1429
FblI GTMKAC 1 cut(s) 173
FspBI CTAG 4 cut(s) 870, 1386, 1802, 2157
GlaI GCGC 1 cut(s) 451
GsaI CCCAGC 2 cut(s) 2045, 2081
HaeII RGCGCY 1 cut(s) 453
HaeIII GGCC 4 cut(s) 422, 1283, 1843, 2748
HapII CCGG 6 cut(s) 134, 146, 152, 1018, 1896, 2366
HgaI GACGC 1 cut(s) 944
HhaI GCGC 1 cut(s) 452
Hin1I GRCGYC 1 cut(s) 335
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HincII GTYRAC 3 cut(s) 1273, 1591, 1726
HindII GTYRAC 3 cut(s) 1273, 1591, 1726
HinfI GANTC 6 cut(s) 517, 742, 1214, 1233, 1907, 2153
HpaI GTTAAC 1 cut(s) 1591
HpaII CCGG 6 cut(s) 134, 146, 152, 1018, 1896, 2366
HphI GGTGA 5 cut(s) 218, 539, 849, 1367, 1701
Hpy166II GTNNAC 7 cut(s) 174, 572, 857, 1273, 1499, 1591, 1726
Hpy8I GTNNAC 7 cut(s) 174, 572, 857, 1273, 1499, 1591, 1726
HpyAV CCTTC 9 cut(s) 219, 258, 518, 1535, 1852, 2201, 2233, 2396, 2656
HpyCH4III ACNGT 8 cut(s) 25, 171, 286, 539, 906, 1199, 1318, 1469
HpyCH4IV ACGT 2 cut(s) 335, 1169
HpyF10VI GCNNNNNNNGC 8 cut(s) 70, 428, 809, 873, 926, 1127, 1683, 2375
HpySE526I ACGT 2 cut(s) 335, 1169
Hsp92I GRCGYC 1 cut(s) 335
HspAI GCGC 1 cut(s) 450
KroI GCCGGC 1 cut(s) 2365
KroNI GCCGGC 1 cut(s) 2367
Ksp22I TGATCA 2 cut(s) 1357, 1429
KspAI GTTAAC 1 cut(s) 1591
Kzo9I GATC 9 cut(s) 380, 490, 529, 1298, 1357, 1375, 1429, 1792, 1798
LmnI GCTCC 5 cut(s) 148, 376, 881, 2335, 2470
Lsp1109I GCAGC 8 cut(s) 142, 860, 1045, 1993, 2211, 2495, 2592, 2681
LweI GCATC 9 cut(s) 314, 352, 758, 821, 907, 1315, 2153, 2347, 2521
MaeI CTAG 4 cut(s) 870, 1386, 1802, 2157
MaeII ACGT 2 cut(s) 335, 1169
MaeIII GTNAC 4 cut(s) 1013, 1020, 1193, 2440
MalI GATC 9 cut(s) 382, 492, 531, 1300, 1359, 1377, 1431, 1794, 1800
MbiI CCGCTC 1 cut(s) 1771
MboI GATC 9 cut(s) 380, 490, 529, 1298, 1357, 1375, 1429, 1792, 1798
MfeI CAATTG 1 cut(s) 2651
MflI RGATCY 4 cut(s) 380, 490, 1298, 1798
MlyI GAGTC 1 cut(s) 1916
Mph1103I ATGCAT 1 cut(s) 307
MroNI GCCGGC 1 cut(s) 2365
MroXI GAANNNNTTC 3 cut(s) 1545, 2190, 2250
MseI TTAA 8 cut(s) 551, 1086, 1139, 1260, 1590, 1614, 1833, 2058
MslI CAYNNNNRTG 4 cut(s) 204, 862, 1059, 1604
MspA1I CMGCKG 5 cut(s) 431, 1721, 2224, 2350, 2699
MspI CCGG 6 cut(s) 134, 146, 152, 1018, 1896, 2366
MspR9I CCNGG 2 cut(s) 1152, 2451
MunI CAATTG 1 cut(s) 2651
Mva1269I GAATGC 4 cut(s) 43, 307, 845, 869
MvaI CCWGG 2 cut(s) 1152, 2451
MwoI GCNNNNNNNGC 8 cut(s) 70, 428, 809, 873, 926, 1127, 1683, 2375
NaeI GCCGGC 1 cut(s) 2367
NcoI CCATGG 1 cut(s) 619
NdeII GATC 9 cut(s) 380, 490, 529, 1298, 1357, 1375, 1429, 1792, 1798
NgoMIV GCCGGC 1 cut(s) 2365
NheI GCTAGC 1 cut(s) 1385
NlaIV GGNNCC 8 cut(s) 138, 150, 372, 996, 1900, 2466, 2728, 2767
NmeAIII GCCGAG 1 cut(s) 2774
NmuCI GTSAC 3 cut(s) 1020, 1193, 2440
NsiI ATGCAT 1 cut(s) 307
NspI RCATGY 1 cut(s) 1603
PagI TCATGA 1 cut(s) 2271
PcsI WCGNNNNNNNCGW 1 cut(s) 1275
PctI GAATGC 4 cut(s) 43, 307, 845, 869
PdiI GCCGGC 1 cut(s) 2367
PdmI GAANNNNTTC 3 cut(s) 1545, 2190, 2250
PfeI GAWTC 5 cut(s) 517, 742, 1214, 1233, 2153
PflMI CCANNNNNTGG 1 cut(s) 434
PinAI ACCGGT 1 cut(s) 1017
PleI GAGTC 1 cut(s) 1915
PpsI GAGTC 1 cut(s) 1915
Psp6I CCWGG 2 cut(s) 1150, 2449
PspEI GGTNACC 1 cut(s) 1013
PspFI CCCAGC 2 cut(s) 2041, 2077
PspGI CCWGG 2 cut(s) 1150, 2449
PspN4I GGNNCC 8 cut(s) 138, 150, 372, 996, 1900, 2466, 2728, 2767
PspPI GGNCC 1 cut(s) 1282
PsrI GAACNNNNNNTAC 2 cut(s) 2701, 2733
PstI CTGCAG 1 cut(s) 1084
PstNI CAGNNNCTG 3 cut(s) 331, 1447, 2762
PsuI RGATCY 4 cut(s) 380, 490, 1298, 1798
PvuII CAGCTG 3 cut(s) 431, 1721, 2699
RsaI GTAC 4 cut(s) 571, 1134, 1938, 2424
RsaNI GTAC 4 cut(s) 570, 1133, 1937, 2423
RseI CAYNNNNRTG 4 cut(s) 204, 862, 1059, 1604
SaqAI TTAA 8 cut(s) 551, 1086, 1139, 1260, 1590, 1614, 1833, 2058
Sau3AI GATC 9 cut(s) 380, 490, 529, 1298, 1357, 1375, 1429, 1792, 1798
Sau96I GGNCC 1 cut(s) 1282
SchI GAGTC 1 cut(s) 1916
ScrFI CCNGG 2 cut(s) 1152, 2451
SfaNI GCATC 9 cut(s) 314, 352, 758, 821, 907, 1315, 2153, 2347, 2521
SfcI CTRYAG 3 cut(s) 174, 1080, 1837
SmiMI CAYNNNNRTG 4 cut(s) 204, 862, 1059, 1604
SmlI CTYRAG 3 cut(s) 183, 1568, 2036
SmoI CTYRAG 3 cut(s) 183, 1568, 2036
SspMI CTAG 4 cut(s) 870, 1386, 1802, 2157
StyD4I CCNGG 2 cut(s) 1150, 2449
StyI CCWWGG 3 cut(s) 366, 619, 879
TaaI ACNGT 8 cut(s) 25, 171, 286, 539, 906, 1199, 1318, 1469
TaiI ACGT 2 cut(s) 338, 1172
TaqI TCGA 3 cut(s) 1236, 2090, 2237
TatI WGTACW 2 cut(s) 1132, 1936
TauI GCSGC 4 cut(s) 76, 425, 2224, 2381
TfiI GAWTC 5 cut(s) 517, 742, 1214, 1233, 2153
Tru1I TTAA 8 cut(s) 551, 1086, 1139, 1260, 1590, 1614, 1833, 2058
Tru9I TTAA 8 cut(s) 551, 1086, 1139, 1260, 1590, 1614, 1833, 2058
TscAI CASTG 4 cut(s) 30, 1029, 1693, 1909
TseFI GTSAC 3 cut(s) 1020, 1193, 2440
TseI GCWGC 8 cut(s) 155, 873, 1033, 2006, 2224, 2483, 2605, 2669
Tsp45I GTSAC 3 cut(s) 1020, 1193, 2440
TspGWI ACGGA 2 cut(s) 989, 1608
TspRI CASTG 4 cut(s) 30, 1029, 1693, 1909
Van91I CCANNNNNTGG 1 cut(s) 434
XagI CCTNNNNNAGG 1 cut(s) 719
XapI RAATTY 8 cut(s) 214, 318, 472, 1404, 1534, 2053, 2327, 2557
XceI RCATGY 1 cut(s) 1603
XmiI GTMKAC 1 cut(s) 173
XmnI GAANNNNTTC 3 cut(s) 1545, 2190, 2250
XspI CTAG 4 cut(s) 870, 1386, 1802, 2157
ZraI GACGTC 1 cut(s) 336
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.