MD07G1072700.v1.1

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005505
Physical Location & Seq
Reverse (-)
6876894 .. 6889727
12834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1072700.v1.1.491

Sequence Viewer

Length: 2877 bp
ATGGCCGATATTAGGAAGTGGTTTATGAAGTCGCACGACAAGGGAAACACCAAAAAACCGGAGGCGACCGTTTCACAGAAGACTGAGCCTAAAGAACCAGTGCAAGGAGGTCAAGAAAATTCTGGCAGGAGGAAAACTAGCAAGTATTTCCCCACAGATAAGCCAAAAGATGAAAAAGGAACAACTGAAGTTCCAGCCAAAAGAAAGCCTCACAAGGATCCTGATGAATCTATTAAACCCTCACCTGCAAAAAAAGCTCACAAAGTTGATGTTGACGACGATGACGATTTTGTCTCTTCCAATTCGAAGAAGAAATCAGTGTATGCCACTCCTAGTAAGAAATTGAAGAGCACGTCTGGCACAGGAATCCCACAGAAGGTTATGGCTAATGATGAAGCTGGCGATGAAGATGAAAAGGTTGCCGAATCATCCCTCAAGCCAGCTGGTCGGGGTCGTGGTGGAAGAGGTGCTTCGGCAGTTCCAGCTGGTGGAAGAGGCAGGGGCGCTGGACGAGGCGGATTTATGAATTATGGAGAAAGGAAAGATCCTCCACACAAGGGAGAAAAGGAAGTTCCTGAAGGTGCTCCTAATTGTTTAGCTGGCTTGACTTTTGTAATTAGTGGTACACTTGACAGTTTGGAAAGAGAAGAAGCAGAGGATTTGATCAAACGTCATGGCGGTCGTATTACTGGATCTGTCAGCAAGAAAACGAACTATCTTTTATGTGATGAAGATATTGAGGGAAGGAAATCCTCCAAAGCCAAAGAACTTGGTACGGCTTTTCTTACCGAGGATGGATTGTTTGGTAAGATTCGGGCGTCTATTGGTGCTAAAGTACCTGTACAAGAAGCAAAGAAATCTGTGGATGATGCTGCAGCAGCATCTTTGCCCAGTAAAAGCCCTAAGAAAGTAACATTGAAGAATTGCACCGGAAGCTTGTTGGCATCAAGTGCATCTAGCAAACAGTTGCAGTCAGATGCCTCCCTTGCTAGGCGTAAGAAGCAAACTACCGAGCATTCTACTTTTACTTGGACAGAAAAATATAGGCCAAAGGTTCCTAACGACATTGTTGGTAATCAATCACTGGTAAAGCAGCTTCATGATTGGTTGTCACATTGGAATGAGCAATTTCTTGATACTGGAGATAAGAAAAAAGGAAAAAACCCAATCAATTCTGGTGCAAAAAAGGCTGTACTTTTAAGTGGAACACCTGGTATAGGGAAAACGACGTCTGCAAAGTTAGTCAGCCAGATGCTTGGTTTCCAGGCAATTGAGGTAAATGCTAGTGACAGTCGTGGGAAAGCTGATTCAAAGATTGAAAAGGGAATTGGTGGAAGCAATGCAAATTCAATTAAAGAGCTTGTCAGCAACAAGGGCCTGAGCATGGATGGATCAAAGCATCCAAAAACTGTGCTGATTATGGATGAGGTTGATGGGATGTCTGCTGGAGATCGGGGAGGAGTTGCTGATCTTATTGCAAGCATAAAGATTTCAAAAATTCCTATCATCTGCATTTGTAATGATCGTTACAGTCAGAAACTGAAAAGTCTTGTGAACTACTGTTTGCTTCTCAGCTTTCGCAAACCTACCAAGCAACAGATGGCAAAGAGGTTGATGCAAGTTGCAAATGCTGAAGGCCTTCAAGTTAATGAGATTGCTCTTGAGGAACTTGCAGAAAAAGTTAATGGAGACATGCGAATGGCAGTTAACCAATTGCAATATATGAGCCTCTCAATGTCTGTCATTAAATATGATGATGTACGGCAGCGTTTATTAAGCAGTTCAAAGGATGAAGATATTTCACCATTCACAGCTGTTGACAAGCTGTTTGGTTTTAATGCTGGAAAGCTGCGAATGGATGAGCGAGTTGACCTGAGCATGAGTGATCCTGACCTAGTCCCTCTTCTAATCCAGGAAAATTATGTCAACTATAGGCCAAGTGCGGCTGTTAAAGATGATAGTGGGATTAAACGTCTGAACTTGATTGCCCGTGCCGCTGAGTCTATTGGCAATGGAGATATTTTCAATGTACAGATTAGGAAATATCGGCAGTGGCAGCTTTCCCAAAGTGCTTGTCTTTCATCCTCTATAATTCCTGCTGCATTGTTGCGGGGGCAAAGAGAGACACTTGAGCAGGGAGAAAGGAATTTTAATAGATTTGGAGGGTGGCTGGGAAAGAACTCAACATTAGGAAAGAATCGTAGGCTCTTGGAAGATTTGCATGTCCATCTTCTTGCTTCTCGTGAATCTAGTTCGGGGAGGGAAACAGTGCGAGTTGAATACCTTTCTCTTCTTCTGAAACGATTGACTGTGCCTCTCCGTGAACTGCCTAAGGATGAAGCAGTGCAGGAAGTTGTGGAGTTCATGAATACTTACTCCATTAGTCAGGATGACTTTGATACTATTGTGGAGTTATCCAAATATCAGGGGCATCCAAATCCGCTAGATGGCATAGTGCCTGCTGTAAAAGCAGCTCTGACAAAAGCATACAAAGAAGGAAGCAAAACAAGGATGGTACGAGCTGCAGATTTTGTCACTATTCCTGGAATGAAAAAGGCCCCTAAGAAGCGGATTGCTGCTCTTTTAGAACCATCTGATGATGCAATTGGAGAGAACAATGATGATACTTTGGTTCAGAGTGAAGATGAGAATTCGTCAGATACAGAGGACTTGGAAGATTCTGCTGTTGGTGAGAAGCTGCAAAAGGAACTGCAAAGCTTGAACACAAAAGGAGTGCAAGTACAATTCGATTTGAAGGGTGCGCCAAATACAAGTGCAAAGAAGACACCAGCTGGCAGAGGTAGAGGTGGTTCTGCTGCTGCCAGCGTGAAAAAGGGCGGCCGAGGTTCCGGAGCTGGTGCCAAGAGAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000166 GO:0000280 GO:0000712 GO:0000723 GO:0000731 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005663 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006278 GO:0006281 GO:0006283 GO:0006289 GO:0006296 GO:0006297 GO:0006301 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008047 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010833 GO:0016043 GO:0017076 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019985 GO:0022402 GO:0022414 GO:0030234 GO:0030554 GO:0031056 GO:0031060 GO:0031323 GO:0031326 GO:0031399 GO:0031935 GO:0031974 GO:0031981 GO:0032200 GO:0032201 GO:0032268 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033043 GO:0033044 GO:0033260 GO:0033554 GO:0033683 GO:0033993 GO:0034641 GO:0034645 GO:0034654 GO:0035639 GO:0035825 GO:0036094 GO:0042221 GO:0042276 GO:0042592 GO:0042769 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044786 GO:0045132 GO:0046483 GO:0048285 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051246 GO:0051252 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051570 GO:0051606 GO:0051716 GO:0060249 GO:0060255 GO:0060968 GO:0061982 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070987 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:0097305 GO:0097367 GO:0098772 GO:0098813 GO:0140013 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902275 GO:1903046 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
AAA BRCT RFC1

Protein Analysis

959

Amino Acids

104.14

Weight (kDa)

9.42

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRCT PF00533 196 - 268 3e-16 BRCA1 C Terminus (BRCT) domain
PTCB-BRCT PF12738 211 - 259 4.4e-06 twin BRCT domain
AAA PF00004 398 - 508 2.2e-11 ATPase family associated with various cellular activities (AAA)
AAA_lid_RFC1 PF25361 532 - 588 4.9e-10 RFC1-like, AAA+ ATPase lid domain
RCF1-5-like_lid PF21960 536 - 577 1.6e-06 RCF1/5-like, AAA+ ATPase lid domain
RFC1 PF08519 669 - 831 1.7e-40 Replication factor RFC1 C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 253
AasI GACNNNNNNGTC 1 cut(s) 290
AatII GACGTC 1 cut(s) 1232
Acc36I ACCTGC 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 2858
AccB7I CCANNNNNTGG 1 cut(s) 488
AccIII TCCGGA 1 cut(s) 2849
AciI CCGC 8 cut(s) 516, 678, 1943, 1995, 2110, 2441, 2569, 2838
AclWI GGATC 6 cut(s) 212, 225, 539, 700, 1399, 1880
AcoI YGGCCR 2 cut(s) 3, 2839
AcsI RAATTY 5 cut(s) 118, 1345, 1497, 2146, 2650
AcuI CTGAAG 3 cut(s) 207, 597, 1653
AcyI GRCGYC 2 cut(s) 818, 1229
AfaI GTAC 9 cut(s) 625, 775, 837, 843, 1194, 1761, 2031, 2517, 2742
AfiI CCNNNNNNNGG 9 cut(s) 12, 104, 376, 488, 557, 990, 1217, 1384, 2447
AjiI CACGTC 1 cut(s) 354
AjnI CCWGG 4 cut(s) 1210, 1263, 1911, 2542
AjuI GAANNNNNNNTTGG 2 cut(s) 1311, 1343
Alw21I GWGCWC 2 cut(s) 353, 586
Alw26I GTCTC 3 cut(s) 298, 1683, 2117
AlwI GGATC 6 cut(s) 212, 225, 539, 700, 1399, 1880
AlwNI CAGNNNCTG 1 cut(s) 1540
Aor13HI TCCGGA 1 cut(s) 2849
AoxI GGCC 7 cut(s) 3, 1046, 1375, 1636, 1934, 2556, 2839
ApoI RAATTY 5 cut(s) 118, 1345, 1497, 2146, 2650
ArsI GACNNNNNNTTYG 4 cut(s) 1811, 1843, 2059, 2091
Asp700I GAANNNNTTC 1 cut(s) 1638
AspLEI GCGC 2 cut(s) 506, 2764
AspS9I GGNCC 2 cut(s) 1375, 2557
AsuHPI GGTGA 3 cut(s) 234, 1794, 2702
AsuII TTCGAA 1 cut(s) 305
AxyI CCTNAGG 1 cut(s) 2331
BaeI ACNNNNGTAYC 4 cut(s) 2391, 2424, 2616, 2649
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 1 cut(s) 2858
BarI GAAGNNNNNNTAC 2 cut(s) 1080, 1112
BauI CACGAG 1 cut(s) 2241
BbsI GAAGAC 2 cut(s) 86, 2789
Bbv12I GWGCWC 2 cut(s) 353, 586
BccI CCATC 8 cut(s) 788, 1382, 1427, 1594, 2235, 2441, 2506, 2599
BceAI ACGGC 2 cut(s) 792, 1778
BciT130I CCWGG 4 cut(s) 1212, 1265, 1913, 2544
BclI TGATCA 1 cut(s) 663
BcoDI GTCTC 3 cut(s) 298, 1683, 2117
BfaI CTAG 8 cut(s) 138, 333, 957, 990, 1284, 1895, 2250, 2444
BfmI CTRYAG 3 cut(s) 873, 1930, 2523
BfoI RGCGCY 1 cut(s) 507
BfuAI ACCTGC 1 cut(s) 253
Bme1390I CCNGG 4 cut(s) 1212, 1265, 1913, 2544
BmgBI CACGTC 1 cut(s) 354
BmgT120I GGNCC 2 cut(s) 1375, 2557
BmiI GGNNCC 5 cut(s) 219, 1056, 2559, 2848, 2860
BmrFI CCNGG 4 cut(s) 1212, 1265, 1913, 2544
BmrI ACTGGG 1 cut(s) 885
BmuI ACTGGG 1 cut(s) 885
BpiI GAAGAC 2 cut(s) 86, 2789
BpmI CTGGAG 2 cut(s) 1161, 1467
Bpu10I CCTNAGC 2 cut(s) 1379, 1874
Bpu14I TTCGAA 1 cut(s) 305
BpuEI CTTGAG 3 cut(s) 419, 1682, 2150
BsaHI GRCGYC 2 cut(s) 818, 1229
BsaJI CCNNGG 2 cut(s) 789, 2842
BsaWI WCCGGW 3 cut(s) 58, 929, 2849
BsaXI ACNNNNNCTCC 2 cut(s) 2724, 2754
Bsc4I CCNNNNNNNGG 9 cut(s) 12, 104, 376, 488, 557, 990, 1217, 1384, 2447
Bse1I ACTGG 5 cut(s) 98, 694, 891, 1089, 1144
Bse21I CCTNAGG 1 cut(s) 2331
Bse3DI GCAATG 2 cut(s) 1345, 2017
BseAI TCCGGA 1 cut(s) 2849
BseBI CCWGG 4 cut(s) 1212, 1265, 1913, 2544
BseDI CCNNGG 2 cut(s) 789, 2842
BseLI CCNNNNNNNGG 9 cut(s) 12, 104, 376, 488, 557, 990, 1217, 1384, 2447
BseMI GCAATG 2 cut(s) 1345, 2017
BseMII CTCAG 5 cut(s) 75, 1370, 1585, 1865, 1989
BseNI ACTGG 5 cut(s) 98, 694, 891, 1089, 1144
BseRI GAGGAG 1 cut(s) 1473
BseX3I CGGCCG 1 cut(s) 2839
BseYI CCCAGC 1 cut(s) 2170
BsgI GTGCAG 1 cut(s) 2366
Bsh1285I CGRYCG 3 cut(s) 69, 682, 2842
BshFI GGCC 7 cut(s) 5, 1048, 1377, 1638, 1936, 2558, 2841
BshNI GGYRCC 1 cut(s) 2858
BsiEI CGRYCG 3 cut(s) 69, 682, 2842
BsiHKAI GWGCWC 2 cut(s) 353, 586
BsiSI CCGG 3 cut(s) 59, 930, 2850
BslFI GGGAC 1 cut(s) 1883
BslI CCNNNNNNNGG 9 cut(s) 12, 104, 376, 488, 557, 990, 1217, 1384, 2447
BsmAI GTCTC 3 cut(s) 298, 1683, 2117
BsmFI GGGAC 1 cut(s) 1883
BsmI GAATGC 1 cut(s) 1015
BsnI GGCC 7 cut(s) 5, 1048, 1377, 1638, 1936, 2558, 2841
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 353, 586
Bsp13I TCCGGA 1 cut(s) 2849
Bsp1407I TGTACA 2 cut(s) 841, 2029
Bsp143I GATC 9 cut(s) 217, 544, 663, 692, 1391, 1450, 1468, 1522, 1885
BspACI CCGC 8 cut(s) 516, 678, 1943, 1995, 2110, 2441, 2569, 2838
BspANI GGCC 7 cut(s) 5, 1048, 1377, 1638, 1936, 2558, 2841
BspCNI CTCAG 5 cut(s) 76, 1371, 1584, 1866, 1990
BspEI TCCGGA 1 cut(s) 2849
BspHI TCATGA 2 cut(s) 1099, 2364
BspLI GGNNCC 5 cut(s) 219, 1056, 2559, 2848, 2860
BspMAI CTGCAG 2 cut(s) 877, 2527
BspMI ACCTGC 1 cut(s) 253
BspPI GGATC 6 cut(s) 212, 225, 539, 700, 1399, 1880
BspQI GCTCTTC 1 cut(s) 341
BspT104I TTCGAA 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 2858
BsrDI GCAATG 2 cut(s) 1345, 2017
BsrGI TGTACA 2 cut(s) 841, 2029
BsrI ACTGG 5 cut(s) 98, 694, 891, 1089, 1144
BssECI CCNNGG 2 cut(s) 789, 2842
BssMI GATC 9 cut(s) 217, 544, 663, 692, 1391, 1450, 1468, 1522, 1885
BssNI GRCGYC 2 cut(s) 818, 1229
BssSI CACGAG 1 cut(s) 2241
Bst2BI CACGAG 1 cut(s) 2241
Bst2UI CCWGG 4 cut(s) 1212, 1265, 1913, 2544
Bst4CI ACNGT 9 cut(s) 70, 635, 966, 1292, 1411, 1532, 1562, 2269, 2311
Bst6I CTCTTC 6 cut(s) 301, 341, 457, 487, 1908, 2295
BstACI GRCGYC 2 cut(s) 818, 1229
BstAPI GCANNNNNTGC 1 cut(s) 950
BstAUI TGTACA 2 cut(s) 841, 2029
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 7 cut(s) 400, 441, 601, 1480, 2460, 2794, 2824
BstDEI CTNAG 8 cut(s) 84, 903, 1379, 1571, 1874, 1998, 2331, 2562
BstENI CCTNNNNNAGG 1 cut(s) 1215
BstH2I RGCGCY 1 cut(s) 507
BstHHI GCGC 2 cut(s) 506, 2764
BstKTI GATC 9 cut(s) 220, 547, 666, 695, 1394, 1453, 1471, 1525, 1888
BstMAI GTCTC 3 cut(s) 298, 1683, 2117
BstMBI GATC 9 cut(s) 217, 544, 663, 692, 1391, 1450, 1468, 1522, 1885
BstMCI CGRYCG 3 cut(s) 69, 682, 2842
BstNI CCWGG 4 cut(s) 1212, 1265, 1913, 2544
BstNSI RCATGY 2 cut(s) 1696, 2225
BstSCI CCNGG 4 cut(s) 1210, 1263, 1911, 2542
BstSFI CTRYAG 3 cut(s) 873, 1930, 2523
BstV2I GAAGAC 2 cut(s) 86, 2789
BstX2I RGATCY 3 cut(s) 217, 544, 692
BstXI CCANNNNNNTGG 1 cut(s) 1256
BstYI RGATCY 3 cut(s) 217, 544, 692
BstZI CGGCCG 1 cut(s) 2839
Bsu36I CCTNAGG 1 cut(s) 2331
BsuRI GGCC 7 cut(s) 5, 1048, 1377, 1638, 1936, 2558, 2841
BtgZI GCGATG 1 cut(s) 417
BtrI CACGTC 1 cut(s) 354
BtsI GCAGTG 2 cut(s) 2057, 2349
BtsIMutI CAGTG 6 cut(s) 105, 324, 1082, 2057, 2274, 2349
BveI ACCTGC 1 cut(s) 253
Cac8I GCNNGC 7 cut(s) 400, 441, 601, 1480, 2460, 2794, 2824
CaiI CAGNNNCTG 1 cut(s) 1540
CciI TCATGA 2 cut(s) 1099, 2364
CfoI GCGC 2 cut(s) 506, 2764
Cfr13I GGNCC 2 cut(s) 1375, 2557
CseI GACGC 1 cut(s) 807
CsiI ACCWGGT 1 cut(s) 1210
Csp6I GTAC 9 cut(s) 624, 774, 836, 842, 1193, 1760, 2030, 2516, 2741
CviAII CATG 7 cut(s) 674, 1100, 1384, 1693, 1879, 2222, 2365
CviQI GTAC 9 cut(s) 624, 774, 836, 842, 1193, 1760, 2030, 2516, 2741
DdeI CTNAG 8 cut(s) 84, 903, 1379, 1571, 1874, 1998, 2331, 2562
DpnI GATC 9 cut(s) 219, 546, 665, 694, 1393, 1452, 1470, 1524, 1887
DpnII GATC 9 cut(s) 217, 544, 663, 692, 1391, 1450, 1468, 1522, 1885
DrdI GACNNNNNNGTC 1 cut(s) 290
DseDI GACNNNNNNGTC 1 cut(s) 290
EaeI YGGCCR 2 cut(s) 3, 2839
EagI CGGCCG 1 cut(s) 2839
Eam1104I CTCTTC 6 cut(s) 301, 341, 457, 487, 1908, 2295
EarI CTCTTC 6 cut(s) 301, 341, 457, 487, 1908, 2295
EciI GGCGGA 1 cut(s) 531
EclXI CGGCCG 1 cut(s) 2839
Eco147I AGGCCT 1 cut(s) 1638
Eco52I CGGCCG 1 cut(s) 2839
Eco57I CTGAAG 3 cut(s) 207, 597, 1653
Eco81I CCTNAGG 1 cut(s) 2331
EcoNI CCTNNNNNAGG 1 cut(s) 1215
EcoO109I RGGNCCY 2 cut(s) 1375, 2557
EcoRI GAATTC 1 cut(s) 2650
EcoRII CCWGG 4 cut(s) 1210, 1263, 1911, 2542
FaeI CATG 7 cut(s) 677, 1103, 1387, 1696, 1882, 2225, 2368
FalI AAGNNNNNCTT 2 cut(s) 454, 486
FaqI GGGAC 1 cut(s) 1883
FatI CATG 7 cut(s) 673, 1099, 1383, 1692, 1878, 2221, 2364
FauI CCCGC 1 cut(s) 2103
FbaI TGATCA 1 cut(s) 663
FspBI CTAG 8 cut(s) 138, 333, 957, 990, 1284, 1895, 2250, 2444
GlaI GCGC 2 cut(s) 505, 2763
GsaI CCCAGC 1 cut(s) 2174
GsuI CTGGAG 2 cut(s) 1161, 1467
HaeII RGCGCY 1 cut(s) 507
HaeIII GGCC 7 cut(s) 5, 1048, 1377, 1638, 1936, 2558, 2841
HapII CCGG 3 cut(s) 59, 930, 2850
HgaI GACGC 1 cut(s) 807
HhaI GCGC 2 cut(s) 506, 2764
Hin1I GRCGYC 2 cut(s) 818, 1229
Hin1II CATG 7 cut(s) 677, 1103, 1387, 1696, 1882, 2225, 2368
Hin6I GCGC 2 cut(s) 504, 2762
HinP1I GCGC 2 cut(s) 504, 2762
HincII GTYRAC 5 cut(s) 274, 1708, 1819, 1870, 1927
HindII GTYRAC 5 cut(s) 274, 1708, 1819, 1870, 1927
HindIII AAGCTT 2 cut(s) 934, 2716
HinfI GANTC 9 cut(s) 227, 366, 425, 811, 1307, 2000, 2197, 2246, 2678
HpaI GTTAAC 1 cut(s) 1708
HpaII CCGG 3 cut(s) 59, 930, 2850
HphI GGTGA 3 cut(s) 234, 1794, 2702
Hpy166II GTNNAC 8 cut(s) 274, 626, 1555, 1708, 1819, 1870, 1927, 2324
Hpy188I TCNGA 8 cut(s) 976, 1536, 1977, 2298, 2478, 2596, 2637, 2659
Hpy8I GTNNAC 8 cut(s) 274, 626, 1555, 1708, 1819, 1870, 1927, 2324
Hpy99I CGWCG 2 cut(s) 281, 1231
HpyAV CCTTC 7 cut(s) 370, 572, 738, 1628, 1649, 2489, 2749
HpyCH4III ACNGT 9 cut(s) 70, 635, 966, 1292, 1411, 1532, 1562, 2269, 2311
HpyCH4IV ACGT 4 cut(s) 353, 670, 1229, 1972
HpyF3I CTNAG 8 cut(s) 84, 903, 1379, 1571, 1874, 1998, 2331, 2562
HpySE526I ACGT 4 cut(s) 353, 670, 1229, 1972
Hsp92I GRCGYC 2 cut(s) 818, 1229
Hsp92II CATG 7 cut(s) 677, 1103, 1387, 1696, 1882, 2225, 2368
HspAI GCGC 2 cut(s) 504, 2762
Kpn2I TCCGGA 1 cut(s) 2849
Ksp22I TGATCA 1 cut(s) 663
KspAI GTTAAC 1 cut(s) 1708
Kzo9I GATC 9 cut(s) 217, 544, 663, 692, 1391, 1450, 1468, 1522, 1885
LguI GCTCTTC 1 cut(s) 341
LmnI GCTCC 2 cut(s) 589, 2852
MabI ACCWGGT 1 cut(s) 1210
MaeI CTAG 8 cut(s) 138, 333, 957, 990, 1284, 1895, 2250, 2444
MaeII ACGT 4 cut(s) 353, 670, 1229, 1972
MaeIII GTNAC 5 cut(s) 910, 1110, 1286, 1526, 2533
MalI GATC 9 cut(s) 219, 546, 665, 694, 1393, 1452, 1470, 1524, 1887
MboI GATC 9 cut(s) 217, 544, 663, 692, 1391, 1450, 1468, 1522, 1885
MfeI CAATTG 3 cut(s) 1269, 1712, 2604
MflI RGATCY 3 cut(s) 217, 544, 692
MhlI GDGCHC 2 cut(s) 353, 586
MlyI GAGTC 1 cut(s) 2009
MroI TCCGGA 1 cut(s) 2849
MroXI GAANNNNTTC 1 cut(s) 1638
MslI CAYNNNNRTG 2 cut(s) 1119, 1697
MspA1I CMGCKG 5 cut(s) 443, 485, 1814, 1997, 2792
MspI CCGG 3 cut(s) 59, 930, 2850
MspR9I CCNGG 4 cut(s) 1212, 1265, 1913, 2544
MunI CAATTG 3 cut(s) 1269, 1712, 2604
Mva1269I GAATGC 1 cut(s) 1015
MvaI CCWGG 4 cut(s) 1212, 1265, 1913, 2544
NdeII GATC 9 cut(s) 217, 544, 663, 692, 1391, 1450, 1468, 1522, 1885
NlaIII CATG 7 cut(s) 677, 1103, 1387, 1696, 1882, 2225, 2368
NlaIV GGNNCC 5 cut(s) 219, 1056, 2559, 2848, 2860
NmeAIII GCCGAG 1 cut(s) 2867
NmuCI GTSAC 3 cut(s) 1110, 1286, 2533
NspI RCATGY 2 cut(s) 1696, 2225
NspV TTCGAA 1 cut(s) 305
PagI TCATGA 2 cut(s) 1099, 2364
PaqCI CACCTGC 1 cut(s) 253
PceI AGGCCT 1 cut(s) 1638
PciSI GCTCTTC 1 cut(s) 341
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PctI GAATGC 1 cut(s) 1015
PdmI GAANNNNTTC 1 cut(s) 1638
PfeI GAWTC 8 cut(s) 227, 366, 425, 811, 1307, 2197, 2246, 2678
PflFI GACNNNGTC 1 cut(s) 1895
PflMI CCANNNNNTGG 1 cut(s) 488
PfoI TCCNGGA 2 cut(s) 1911, 2542
PleI GAGTC 1 cut(s) 2008
PpsI GAGTC 1 cut(s) 2008
Psp6I CCWGG 4 cut(s) 1210, 1263, 1911, 2542
PspFI CCCAGC 1 cut(s) 2170
PspGI CCWGG 4 cut(s) 1210, 1263, 1911, 2542
PspN4I GGNNCC 5 cut(s) 219, 1056, 2559, 2848, 2860
PspPI GGNCC 2 cut(s) 1375, 2557
PsrI GAACNNNNNNTAC 2 cut(s) 2794, 2826
PstI CTGCAG 2 cut(s) 877, 2527
PstNI CAGNNNCTG 1 cut(s) 1540
PsuI RGATCY 3 cut(s) 217, 544, 692
PsyI GACNNNGTC 1 cut(s) 1895
PvuII CAGCTG 4 cut(s) 443, 485, 1814, 2792
RsaI GTAC 9 cut(s) 625, 775, 837, 843, 1194, 1761, 2031, 2517, 2742
RsaNI GTAC 9 cut(s) 624, 774, 836, 842, 1193, 1760, 2030, 2516, 2741
RseI CAYNNNNRTG 2 cut(s) 1119, 1697
SapI GCTCTTC 1 cut(s) 341
Sau3AI GATC 9 cut(s) 217, 544, 663, 692, 1391, 1450, 1468, 1522, 1885
Sau96I GGNCC 2 cut(s) 1375, 2557
SchI GAGTC 1 cut(s) 2009
ScrFI CCNGG 4 cut(s) 1212, 1265, 1913, 2544
SduI GDGCHC 2 cut(s) 353, 586
SexAI ACCWGGT 1 cut(s) 1210
SfcI CTRYAG 3 cut(s) 873, 1930, 2523
SfuI TTCGAA 1 cut(s) 305
SmiMI CAYNNNNRTG 2 cut(s) 1119, 1697
SmlI CTYRAG 3 cut(s) 434, 1661, 2129
SmoI CTYRAG 3 cut(s) 434, 1661, 2129
SseBI AGGCCT 1 cut(s) 1638
SsiI CCGC 8 cut(s) 516, 678, 1943, 1995, 2110, 2441, 2569, 2838
SspMI CTAG 8 cut(s) 138, 333, 957, 990, 1284, 1895, 2250, 2444
StuI AGGCCT 1 cut(s) 1638
StyD4I CCNGG 4 cut(s) 1210, 1263, 1911, 2542
TaaI ACNGT 9 cut(s) 70, 635, 966, 1292, 1411, 1532, 1562, 2269, 2311
TaiI ACGT 4 cut(s) 356, 673, 1232, 1975
TaqI TCGA 2 cut(s) 305, 2748
TatI WGTACW 4 cut(s) 841, 1192, 2029, 2740
TauI GCSGC 3 cut(s) 1946, 1997, 2841
TfiI GAWTC 8 cut(s) 227, 366, 425, 811, 1307, 2197, 2246, 2678
TscAI CASTG 6 cut(s) 105, 324, 1089, 2057, 2274, 2349
TseFI GTSAC 3 cut(s) 1110, 1286, 2533
Tsp45I GTSAC 3 cut(s) 1110, 1286, 2533
TspGWI ACGGA 1 cut(s) 2309
TspRI CASTG 6 cut(s) 105, 324, 1089, 2057, 2274, 2349
Tth111I GACNNNGTC 1 cut(s) 1895
Van91I CCANNNNNTGG 1 cut(s) 488
XagI CCTNNNNNAGG 1 cut(s) 1215
XapI RAATTY 5 cut(s) 118, 1345, 1497, 2146, 2650
XceI RCATGY 2 cut(s) 1696, 2225
XcmI CCANNNNNNNNNTGG 1 cut(s) 1597
XmnI GAANNNNTTC 1 cut(s) 1638
XspI CTAG 8 cut(s) 138, 333, 957, 990, 1284, 1895, 2250, 2444
ZraI GACGTC 1 cut(s) 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.