AT5G25480
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003990
Physical Location & Seq
Reverse (-)
8869865 .. 8872262
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25480.1

Sequence Viewer

Length: 1152 bp
ATGGCGGAACAAGAATTACAGAGAATCAATGAGAAGAAGCCATGGCAAGTTCTCGAGTTCTACAGTGGAATCGGTGGAATGAGATACTCTTTAATGGCGTCAGGCATCGTCTCAGAAGTAGTAGAAGCTTTTGAGATCAATGATTCTGCTAATGATGTTTATCAACATAATTTCAAACATCGTCCCTATCAGGGGAATATTCAGAGTCTCACTGCTGCTGATTTAGATAAGTATAATGCAGATGCTTGGCTTCTTTCTCCTCCTTGTCAACCTTATACTAGACAAGGCCTTCAGAAACACTCTGGTGATGCTCGGGCTTTTTCGTTTCTCAGGATTCTTGAGCTTATACCGCATACAACAAAGCCTCCACAAATGTTATTTGTAGAGAATGTTGTTGGATTTGAGACTTCGGATACACATATGGAAATGATTGGTACATTAACAAAATTAGATTATGTCACACAAGAGTTTATATTGAGTCCGCTGCAGTTTGGTGTGCCGTATTCAAGACCACGTTATTTCTGCCTGGCCAAAAGAAAACCTCTGCCATTTAAGTCCCAACACAGTAATAACAAACTACTCTGGTCTCCAGACCCGTTATATGGGCGTGATGATCAGGTTGAGTTTGGAAAATGTCAAGCAGAAGAAGGATTGGATAAATTACTTGAGTTTTGCAAACCAGTAGAGAAGTTTCTCGAGTTAGCTGCACATGTTGATGGCGAGCCAAGTAGTGTTGATGACTCTGAAAATGGGTCCAAAGATTGTTGTGGACAAGAAGGCGATTCTGTCCCAGACTCTGTACATCAGTATCTAGTACCAGTTAGTTTGATAGAGAGATGGGGAAACGCAATGGACATTGTTTACCCTGATTCAAAGAGATGTTGTTGTTTCACAAAGAGTTATTATCGATACGTGAAGGGCACAGGTTCCCTATTGGCAACTGTCCAGCCAAAAATCAAGGGTAAGGAATCTTGTTTGAAGGAGCAACGGCTTAGATACTTCACCCCTAGAGAGGTAGCTAACTTTCACTCATTCCCTGAAGATTTTGAGTTCCCCAAGCACATAAGCCTTCGACAAAGATACGCAATGCTTGGAAACAGTTTAAGTGTAGCAGTGGTGGCTCCTCTGCTTCGGTATCTATTCGATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

383

Amino Acids

43.72

Weight (kDa)

5.97

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 16 - 377 7e-40 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 350, 482
AcoI YGGCCR 1 cut(s) 528
AcuI CTGAAG 2 cut(s) 275, 1059
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 3 cut(s) 436, 801, 816
AfiI CCNNNNNNNGG 4 cut(s) 191, 192, 602, 1012
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 4 cut(s) 175, 507, 873, 979
AjnI CCWGG 1 cut(s) 525
AleI CACNNNNGTG 1 cut(s) 303
AluBI AGCT 4 cut(s) 128, 343, 704, 1019
AluI AGCT 4 cut(s) 128, 343, 704, 1019
Alw26I GTCTC 4 cut(s) 115, 212, 398, 591
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 3 cut(s) 53, 312, 695
AoxI GGCC 2 cut(s) 286, 528
ApeKI GCWGC 3 cut(s) 215, 484, 704
AspS9I GGNCC 1 cut(s) 753
AsuHPI GGTGA 2 cut(s) 317, 994
AvaI CYCGRG 3 cut(s) 53, 312, 695
AvaII GGWCC 1 cut(s) 753
BaeGI GKGCMC 1 cut(s) 923
BaeI ACNNNNGTAYC 2 cut(s) 791, 824
BalI TGGCCA 1 cut(s) 530
BbvI GCAGC 3 cut(s) 202, 471, 691
BccI CCATC 2 cut(s) 710, 831
BceAI ACGGC 2 cut(s) 484, 1004
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BciT130I CCWGG 1 cut(s) 527
BciVI GTATCC 1 cut(s) 406
BclI TGATCA 1 cut(s) 613
BcoDI GTCTC 4 cut(s) 115, 212, 398, 591
BfaI CTAG 3 cut(s) 279, 812, 1008
BfmI CTRYAG 2 cut(s) 61, 485
BfuI GTATCC 1 cut(s) 406
BisI GCNGC 3 cut(s) 216, 485, 705
BlsI GCNGC 3 cut(s) 217, 486, 706
Bme1390I CCNGG 1 cut(s) 527
Bme18I GGWCC 1 cut(s) 753
BmeT110I CYCGRG 3 cut(s) 53, 312, 695
BmgT120I GGNCC 1 cut(s) 753
BmiI GGNNCC 3 cut(s) 754, 928, 1122
BmrFI CCNGG 1 cut(s) 527
BmsI GCATC 3 cut(s) 114, 232, 298
BpmI CTGGAG 1 cut(s) 573
BpuEI CTTGAG 2 cut(s) 359, 686
Bsa29I ATCGAT 1 cut(s) 907
BsaAI YACGTR 1 cut(s) 913
BsaBI GATNNNNATC 1 cut(s) 159
BsaHI GRCGYC 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 591
BsaJI CCNNGG 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 349, 379
Bsc4I CCNNNNNNNGG 4 cut(s) 191, 192, 602, 1012
Bse1I ACTGG 2 cut(s) 680, 818
Bse3DI GCAATG 2 cut(s) 855, 1092
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 1 cut(s) 527
BseCI ATCGAT 1 cut(s) 907
BseDI CCNNGG 1 cut(s) 41
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 4 cut(s) 191, 192, 602, 1012
BseMI GCAATG 2 cut(s) 855, 1092
BseMII CTCAG 2 cut(s) 126, 343
BseNI ACTGG 2 cut(s) 680, 818
BseRI GAGGAG 2 cut(s) 249, 1113
BseSI GKGCMC 1 cut(s) 923
BseXI GCAGC 3 cut(s) 202, 471, 691
BsgI GTGCAG 1 cut(s) 690
BshFI GGCC 2 cut(s) 288, 530
BshVI ATCGAT 1 cut(s) 907
BsiHKCI CYCGRG 3 cut(s) 53, 312, 695
BslFI GGGAC 3 cut(s) 168, 541, 773
BslI CCNNNNNNNGG 4 cut(s) 191, 192, 602, 1012
BsmAI GTCTC 4 cut(s) 115, 212, 398, 591
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 3 cut(s) 168, 541, 773
BsnI GGCC 2 cut(s) 288, 530
Bso31I GGTCTC 1 cut(s) 591
BsoBI CYCGRG 3 cut(s) 53, 312, 695
Bsp1286I GDGCHC 1 cut(s) 923
Bsp1407I TGTACA 1 cut(s) 799
Bsp143I GATC 2 cut(s) 135, 613
Bsp19I CCATGG 1 cut(s) 41
BspACI CCGC 3 cut(s) 5, 350, 482
BspANI GGCC 2 cut(s) 288, 530
BspCNI CTCAG 2 cut(s) 125, 342
BspDI ATCGAT 1 cut(s) 907
BspLI GGNNCC 3 cut(s) 754, 928, 1122
BspMAI CTGCAG 1 cut(s) 489
BspTNI GGTCTC 1 cut(s) 591
BsrDI GCAATG 2 cut(s) 855, 1092
BsrGI TGTACA 1 cut(s) 799
BsrI ACTGG 2 cut(s) 680, 818
BssECI CCNNGG 1 cut(s) 41
BssMI GATC 2 cut(s) 135, 613
BssNI GRCGYC 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 41
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 4 cut(s) 65, 566, 943, 1100
BstACI GRCGYC 1 cut(s) 98
BstAUI TGTACA 1 cut(s) 799
BstBAI YACGTR 1 cut(s) 913
BstC8I GCNNGC 1 cut(s) 722
BstDEI CTNAG 3 cut(s) 112, 329, 992
BstDSI CCRYGG 1 cut(s) 41
BstKTI GATC 2 cut(s) 138, 616
BstMAI GTCTC 4 cut(s) 115, 212, 398, 591
BstMBI GATC 2 cut(s) 135, 613
BstMWI GCNNNNNNNGC 2 cut(s) 349, 1118
BstNI CCWGG 1 cut(s) 527
BstNSI RCATGY 1 cut(s) 713
BstSCI CCNGG 1 cut(s) 525
BstSFI CTRYAG 2 cut(s) 61, 485
BstSLI GKGCMC 1 cut(s) 923
BstV1I GCAGC 3 cut(s) 202, 471, 691
Bsu15I ATCGAT 1 cut(s) 907
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 2 cut(s) 288, 530
BsuTUI ATCGAT 1 cut(s) 907
BtgI CCRYGG 1 cut(s) 41
BtsI GCAGTG 2 cut(s) 210, 1119
BtsIMutI CAGTG 3 cut(s) 70, 210, 1119
Cac8I GCNNGC 1 cut(s) 722
CaiI CAGNNNCTG 1 cut(s) 797
Cfr13I GGNCC 1 cut(s) 753
ClaI ATCGAT 1 cut(s) 907
CseI GACGC 1 cut(s) 87
Csp6I GTAC 3 cut(s) 435, 800, 815
CviAII CATG 2 cut(s) 42, 710
CviQI GTAC 3 cut(s) 435, 800, 815
DdeI CTNAG 3 cut(s) 112, 329, 992
DpnI GATC 2 cut(s) 137, 615
DpnII GATC 2 cut(s) 135, 613
EaeI YGGCCR 1 cut(s) 528
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 41
Eco147I AGGCCT 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 591
Eco47I GGWCC 1 cut(s) 753
Eco57I CTGAAG 2 cut(s) 275, 1059
Eco88I CYCGRG 3 cut(s) 53, 312, 695
EcoRII CCWGG 1 cut(s) 525
EcoT14I CCWWGG 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 41
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 2 cut(s) 45, 713
FaqI GGGAC 3 cut(s) 168, 541, 773
FatI CATG 2 cut(s) 41, 709
FauNDI CATATG 1 cut(s) 420
FbaI TGATCA 1 cut(s) 613
Fnu4HI GCNGC 3 cut(s) 216, 485, 705
Fsp4HI GCNGC 3 cut(s) 216, 485, 705
FspBI CTAG 3 cut(s) 279, 812, 1008
GluI GCNGC 3 cut(s) 216, 485, 705
GsuI CTGGAG 1 cut(s) 573
HaeIII GGCC 2 cut(s) 288, 530
HgaI GACGC 1 cut(s) 87
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 2 cut(s) 45, 713
HincII GTYRAC 1 cut(s) 269
HindII GTYRAC 1 cut(s) 269
HindIII AAGCTT 1 cut(s) 126
HphI GGTGA 2 cut(s) 317, 994
Hpy166II GTNNAC 3 cut(s) 269, 770, 862
Hpy188I TCNGA 5 cut(s) 115, 204, 294, 412, 745
Hpy188III TCNNGA 7 cut(s) 53, 331, 338, 507, 590, 695, 1149
Hpy8I GTNNAC 3 cut(s) 269, 770, 862
HpyAV CCTTC 6 cut(s) 299, 641, 770, 910, 973, 1079
HpyCH4III ACNGT 4 cut(s) 65, 566, 943, 1100
HpyCH4IV ACGT 2 cut(s) 514, 912
HpyCH4V TGCA 4 cut(s) 239, 487, 675, 707
HpyF10VI GCNNNNNNNGC 2 cut(s) 349, 1118
HpyF3I CTNAG 3 cut(s) 112, 329, 992
HpySE526I ACGT 2 cut(s) 514, 912
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 2 cut(s) 45, 713
Ksp22I TGATCA 1 cut(s) 613
Kzo9I GATC 2 cut(s) 135, 613
LmnI GCTCC 2 cut(s) 982, 1126
Lsp1109I GCAGC 3 cut(s) 202, 471, 691
LweI GCATC 3 cut(s) 114, 232, 298
MaeI CTAG 3 cut(s) 279, 812, 1008
MaeII ACGT 2 cut(s) 514, 912
MaeIII GTNAC 1 cut(s) 457
MalI GATC 2 cut(s) 137, 615
MboI GATC 2 cut(s) 135, 613
MboII GAAGA 3 cut(s) 46, 656, 1052
MhlI GDGCHC 1 cut(s) 923
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 4 cut(s) 14, 169, 446, 659
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 4 cut(s) 214, 487, 734, 788
MmeI TCCRAC 1 cut(s) 376
MnlI CCTC 5 cut(s) 270, 375, 552, 1006, 1134
Mox20I TGGCCA 1 cut(s) 530
MscI TGGCCA 1 cut(s) 530
MseI TTAA 4 cut(s) 92, 440, 552, 1103
MslI CAYNNNNRTG 2 cut(s) 303, 714
Msp20I TGGCCA 1 cut(s) 530
MspA1I CMGCKG 1 cut(s) 484
MspR9I CCNGG 1 cut(s) 527
MvaI CCWGG 1 cut(s) 527
MwoI GCNNNNNNNGC 2 cut(s) 349, 1118
NcoI CCATGG 1 cut(s) 41
NdeI CATATG 1 cut(s) 420
NdeII GATC 2 cut(s) 135, 613
NlaIII CATG 2 cut(s) 45, 713
NlaIV GGNNCC 3 cut(s) 754, 928, 1122
NmuCI GTSAC 1 cut(s) 457
NspI RCATGY 1 cut(s) 713
OliI CACNNNNGTG 1 cut(s) 303
PaeR7I CTCGAG 2 cut(s) 53, 695
PceI AGGCCT 1 cut(s) 288
PciI ACATGT 1 cut(s) 709
PfeI GAWTC 8 cut(s) 24, 69, 143, 334, 782, 869, 968, 1145
PkrI GCNGC 3 cut(s) 217, 486, 706
PleI GAGTC 4 cut(s) 213, 486, 734, 788
PpsI GAGTC 4 cut(s) 213, 486, 734, 788
Ppu21I YACGTR 1 cut(s) 913
PscI ACATGT 1 cut(s) 709
Psp6I CCWGG 1 cut(s) 525
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 3 cut(s) 754, 928, 1122
PspPI GGNCC 1 cut(s) 753
PsrI GAACNNNNNNTAC 1 cut(s) 32
PstI CTGCAG 1 cut(s) 489
PstNI CAGNNNCTG 1 cut(s) 797
RsaI GTAC 3 cut(s) 436, 801, 816
RsaNI GTAC 3 cut(s) 435, 800, 815
RseI CAYNNNNRTG 2 cut(s) 303, 714
SaqAI TTAA 4 cut(s) 92, 440, 552, 1103
SatI GCNGC 3 cut(s) 216, 485, 705
Sau3AI GATC 2 cut(s) 135, 613
Sau96I GGNCC 1 cut(s) 753
SchI GAGTC 4 cut(s) 214, 487, 734, 788
ScrFI CCNGG 1 cut(s) 527
SduI GDGCHC 1 cut(s) 923
SfaNI GCATC 3 cut(s) 114, 232, 298
SfcI CTRYAG 2 cut(s) 61, 485
Sfr274I CTCGAG 2 cut(s) 53, 695
SinI GGWCC 1 cut(s) 753
SlaI CTCGAG 2 cut(s) 53, 695
SmiMI CAYNNNNRTG 2 cut(s) 303, 714
SmlI CTYRAG 4 cut(s) 53, 338, 665, 695
SmoI CTYRAG 4 cut(s) 53, 338, 665, 695
Sse9I AATT 4 cut(s) 14, 169, 446, 659
SseBI AGGCCT 1 cut(s) 288
SsiI CCGC 3 cut(s) 5, 350, 482
SspI AATATT 1 cut(s) 199
SspMI CTAG 3 cut(s) 279, 812, 1008
StuI AGGCCT 1 cut(s) 288
StyD4I CCNGG 1 cut(s) 525
StyI CCWWGG 1 cut(s) 41
TaaI ACNGT 4 cut(s) 65, 566, 943, 1100
TaiI ACGT 2 cut(s) 517, 915
TaqI TCGA 5 cut(s) 54, 696, 907, 1072, 1143
TasI AATT 4 cut(s) 14, 169, 446, 659
TatI WGTACW 1 cut(s) 799
TfiI GAWTC 8 cut(s) 24, 69, 143, 334, 782, 869, 968, 1145
Tru1I TTAA 4 cut(s) 92, 440, 552, 1103
Tru9I TTAA 4 cut(s) 92, 440, 552, 1103
TscAI CASTG 3 cut(s) 70, 217, 1119
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 3 cut(s) 215, 484, 704
Tsp45I GTSAC 1 cut(s) 457
TspRI CASTG 3 cut(s) 70, 217, 1119
VpaK11BI GGWCC 1 cut(s) 753
XceI RCATGY 1 cut(s) 713
XhoI CTCGAG 2 cut(s) 53, 695
XspI CTAG 3 cut(s) 279, 812, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.