Rroxscaffold_7G00169480
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003990
Physical Location & Seq
Reverse (-)
10100368 .. 10103695
3328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00169480.1

Sequence Viewer

Length: 1158 bp
ATGGAGAAGGGTTCTGAGCAGCCATGGAGGGTCCTCGAGTTCTACAGCGGTATTGGTGGCATGAGGTATTCGTTAATGAAGGCGGGAGTGAATGCCAAGGTTGTTGAAGCATTCGACATCAACGACACTGCCAACGATGTTTATCAGCATAACTTCGGACACCGTCCTTATCAGGGTAACATTCAGAGCCTCACTGCAGCTGATCTCGATAGATATGGAGCGAATGTATGGCTTCTGTCTCCTCCTTGCCAACCTTATACTAGACAAGGTCTCCAAAAGCAATCTGGTGATGCTCGGGCATTTTCATTTCTCCAGATTCTTGAGTTGATACCACACACTTTGCGACCTCCAATAATGTTATTTGTGGAAAATGTTGTTGGATTTGAGACATCTGATACACATGGAAAAATGATAGAGATATTGAGAAGAAGTGATTTTGTTACACAGGAGTTCATATTGAGTCCCTTGCAGTTTGGTGTACCATATTCCAGGCCACGCTATTTTTGCCTGGCAAAGAGGAAACCGTCAACCTTTCATAGTCAACTTTTCAATAATCAGCTTCTTTGGTCCCCAAAGCCCTTATTTGGGCATACAAATGCAGATGACAAAGTGTTCTGTGAACTTGATGAGCCACAAGGGAGCTTGGATAAGTTGCTTGAATCTTGTGAGCCAATAGAAAGCTTTCTTGAATTCAAGAATTGCCGTGATCAGCCGAACTTTGTGGACAGCACCACTGTTTATACAGATACCATTGGAGTATTGGACAAAGATGAAGAGAATGGGTGTTGTACTAGCACCTTGGATCAGTACTCTGTTCCCTCAAGCTTGATAGAGAGATGGGGAAGTGCCATGGATATTGTCTACCCGGATTCAAAGCGATGCTGTTGTTTTACAAAAAGTTATTATCGATATGTGAAGGGTACTGGCTCCCTTTTGGCAACTGGGCAGCCAAAGATAAAGGATAAAGCATCTTCACTGAAGGAACAAAGCCTTAGATACTTTACCCCTAGAGAGGTTGCTAATTTGCATTCTTTGCCGGAGAGCTTTCAGTTTCCACAGCAAATAAGCCTTAGACAACGGTATGCATTGCTGGGAAACAGCCTGAGCATCTCAGTGGTTGCTCCTTTACTCCAATATTTATTTGCTGAATCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

385

Amino Acids

43.72

Weight (kDa)

6.03

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 10 - 376 4.9e-34 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 861
AciI CCGC 2 cut(s) 48, 83
AclWI GGATC 1 cut(s) 810
AcsI RAATTY 1 cut(s) 689
AcuI CTGAAG 1 cut(s) 998
AfaI GTAC 4 cut(s) 480, 790, 809, 922
AfiI CCNNNNNNNGG 4 cut(s) 173, 584, 585, 1012
AgsI TTSAA 6 cut(s) 107, 550, 659, 689, 694, 873
AjnI CCWGG 2 cut(s) 488, 507
AjuI GAANNNNNNNTTGG 2 cut(s) 360, 392
AluBI AGCT 6 cut(s) 200, 559, 642, 681, 825, 1044
AluI AGCT 6 cut(s) 200, 559, 642, 681, 825, 1044
Alw26I GTCTC 3 cut(s) 243, 275, 380
AlwI GGATC 1 cut(s) 810
Ama87I CYCGRG 2 cut(s) 35, 294
AoxI GGCC 1 cut(s) 491
ApeKI GCWGC 3 cut(s) 19, 197, 946
ApoI RAATTY 1 cut(s) 689
Asp700I GAANNNNTTC 1 cut(s) 681
AspS9I GGNCC 2 cut(s) 31, 567
AsuC2I CCSGG 1 cut(s) 866
AsuHPI GGTGA 1 cut(s) 299
AvaI CYCGRG 2 cut(s) 35, 294
AvaII GGWCC 2 cut(s) 31, 567
BbvI GCAGC 3 cut(s) 31, 209, 958
BccI CCATC 1 cut(s) 831
BceAI ACGGC 1 cut(s) 687
BciT130I CCWGG 2 cut(s) 490, 509
BclI TGATCA 1 cut(s) 706
BcnI CCSGG 1 cut(s) 866
BcoDI GTCTC 3 cut(s) 243, 275, 380
BfaI CTAG 3 cut(s) 261, 792, 1008
BfmI CTRYAG 2 cut(s) 43, 195
BisI GCNGC 3 cut(s) 20, 198, 947
BlsI GCNGC 3 cut(s) 21, 199, 948
BmcAI AGTACT 1 cut(s) 809
Bme1390I CCNGG 3 cut(s) 490, 509, 866
Bme18I GGWCC 2 cut(s) 31, 567
BmeT110I CYCGRG 2 cut(s) 35, 294
BmgT120I GGNCC 2 cut(s) 31, 567
BmiI GGNNCC 3 cut(s) 32, 569, 928
BmrFI CCNGG 3 cut(s) 490, 509, 866
BmrI ACTGGG 1 cut(s) 951
BmsI GCATC 4 cut(s) 280, 869, 977, 1116
BmuI ACTGGG 1 cut(s) 951
BpmI CTGGAG 1 cut(s) 296
Bpu10I CCTNAGC 1 cut(s) 1103
BpuEI CTTGAG 2 cut(s) 341, 805
BpuMI CCSGG 1 cut(s) 866
Bsa29I ATCGAT 1 cut(s) 907
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 4 cut(s) 23, 96, 798, 849
BsaXI ACNNNNNCTCC 4 cut(s) 210, 240, 255, 285
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 584, 585, 1012
Bse1I ACTGG 2 cut(s) 928, 946
Bse3DI GCAATG 1 cut(s) 1085
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 2 cut(s) 490, 509
BseCI ATCGAT 1 cut(s) 907
BseDI CCNNGG 4 cut(s) 23, 96, 798, 849
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 4 cut(s) 173, 584, 585, 1012
BseMI GCAATG 1 cut(s) 1085
BseMII CTCAG 3 cut(s) 6, 1094, 1125
BseNI ACTGG 2 cut(s) 928, 946
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 3 cut(s) 31, 209, 958
BseYI CCCAGC 1 cut(s) 1090
BshFI GGCC 1 cut(s) 493
BshVI ATCGAT 1 cut(s) 907
BsiHKCI CYCGRG 2 cut(s) 35, 294
BsiSI CCGG 2 cut(s) 866, 1037
BslFI GGGAC 2 cut(s) 447, 553
BslI CCNNNNNNNGG 4 cut(s) 173, 584, 585, 1012
BsmAI GTCTC 3 cut(s) 243, 275, 380
BsmFI GGGAC 2 cut(s) 447, 553
BsmI GAATGC 3 cut(s) 97, 110, 1027
BsnI GGCC 1 cut(s) 493
Bso31I GGTCTC 1 cut(s) 275
BsoBI CYCGRG 2 cut(s) 35, 294
Bsp143I GATC 3 cut(s) 202, 706, 802
Bsp19I CCATGG 2 cut(s) 23, 849
BspACI CCGC 2 cut(s) 48, 83
BspANI GGCC 1 cut(s) 493
BspCNI CTCAG 3 cut(s) 7, 1095, 1124
BspDI ATCGAT 1 cut(s) 907
BspHI TCATGA 1 cut(s) 1154
BspLI GGNNCC 3 cut(s) 32, 569, 928
BspMAI CTGCAG 1 cut(s) 199
BspPI GGATC 1 cut(s) 810
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 1085
BsrI ACTGG 2 cut(s) 928, 946
BssECI CCNNGG 4 cut(s) 23, 96, 798, 849
BssMI GATC 3 cut(s) 202, 706, 802
BssT1I CCWWGG 4 cut(s) 23, 96, 798, 849
Bst2UI CCWGG 2 cut(s) 490, 509
Bst4CI ACNGT 4 cut(s) 164, 525, 736, 1080
Bst6I CTCTTC 1 cut(s) 768
BstAPI GCANNNNNTGC 1 cut(s) 1033
BstDEI CTNAG 5 cut(s) 15, 992, 1070, 1103, 1111
BstDSI CCRYGG 2 cut(s) 23, 849
BstKTI GATC 3 cut(s) 205, 709, 805
BstMAI GTCTC 3 cut(s) 243, 275, 380
BstMBI GATC 3 cut(s) 202, 706, 802
BstMWI GCNNNNNNNGC 2 cut(s) 504, 1033
BstNI CCWGG 2 cut(s) 490, 509
BstSCI CCNGG 3 cut(s) 488, 507, 864
BstSFI CTRYAG 2 cut(s) 43, 195
BstV1I GCAGC 3 cut(s) 31, 209, 958
Bsu15I ATCGAT 1 cut(s) 907
BsuRI GGCC 1 cut(s) 493
BsuTUI ATCGAT 1 cut(s) 907
BtgI CCRYGG 2 cut(s) 23, 849
BtgZI GCGATG 1 cut(s) 892
BtsI GCAGTG 2 cut(s) 126, 192
BtsIMutI CAGTG 5 cut(s) 126, 192, 732, 974, 1119
CciI TCATGA 1 cut(s) 1154
Cfr13I GGNCC 2 cut(s) 31, 567
ClaI ATCGAT 1 cut(s) 907
Csp6I GTAC 4 cut(s) 479, 789, 808, 921
CspCI CAANNNNNGTGG 2 cut(s) 321, 356
CviAII CATG 5 cut(s) 24, 61, 401, 850, 1155
CviQI GTAC 4 cut(s) 479, 789, 808, 921
DdeI CTNAG 5 cut(s) 15, 992, 1070, 1103, 1111
DpnI GATC 3 cut(s) 204, 708, 804
DpnII GATC 3 cut(s) 202, 706, 802
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Eco130I CCWWGG 4 cut(s) 23, 96, 798, 849
Eco31I GGTCTC 1 cut(s) 275
Eco47I GGWCC 2 cut(s) 31, 567
Eco57I CTGAAG 1 cut(s) 998
Eco88I CYCGRG 2 cut(s) 35, 294
EcoO109I RGGNCCY 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 689
EcoRII CCWGG 2 cut(s) 488, 507
EcoT14I CCWWGG 4 cut(s) 23, 96, 798, 849
EcoT22I ATGCAT 1 cut(s) 1087
ErhI CCWWGG 4 cut(s) 23, 96, 798, 849
FaeI CATG 5 cut(s) 27, 64, 404, 853, 1158
FaqI GGGAC 2 cut(s) 447, 553
FatI CATG 5 cut(s) 23, 60, 400, 849, 1154
FauI CCCGC 1 cut(s) 76
FbaI TGATCA 1 cut(s) 706
FblI GTMKAC 1 cut(s) 861
Fnu4HI GCNGC 3 cut(s) 20, 198, 947
Fsp4HI GCNGC 3 cut(s) 20, 198, 947
FspBI CTAG 3 cut(s) 261, 792, 1008
GluI GCNGC 3 cut(s) 20, 198, 947
GsaI CCCAGC 1 cut(s) 1094
GsuI CTGGAG 1 cut(s) 296
HaeIII GGCC 1 cut(s) 493
HapII CCGG 2 cut(s) 866, 1037
Hin1II CATG 5 cut(s) 27, 64, 404, 853, 1158
HincII GTYRAC 2 cut(s) 528, 542
HindII GTYRAC 2 cut(s) 528, 542
HindIII AAGCTT 2 cut(s) 679, 823
HinfI GANTC 5 cut(s) 316, 460, 659, 869, 1148
HpaII CCGG 2 cut(s) 866, 1037
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 6 cut(s) 479, 528, 542, 620, 724, 862
Hpy188I TCNGA 4 cut(s) 16, 158, 186, 394
Hpy188III TCNNGA 6 cut(s) 206, 313, 320, 686, 694, 1155
Hpy8I GTNNAC 6 cut(s) 479, 528, 542, 620, 724, 862
HpyAV CCTTC 3 cut(s) 73, 910, 973
HpyCH4III ACNGT 4 cut(s) 164, 525, 736, 1080
HpyCH4V TGCA 5 cut(s) 197, 469, 599, 1027, 1085
HpyF10VI GCNNNNNNNGC 2 cut(s) 504, 1033
HpyF3I CTNAG 5 cut(s) 15, 992, 1070, 1103, 1111
Hsp92II CATG 5 cut(s) 27, 64, 404, 853, 1158
Ksp22I TGATCA 1 cut(s) 706
Kzo9I GATC 3 cut(s) 202, 706, 802
LmnI GCTCC 4 cut(s) 218, 639, 932, 1126
Lsp1109I GCAGC 3 cut(s) 31, 209, 958
LweI GCATC 4 cut(s) 280, 869, 977, 1116
MaeI CTAG 3 cut(s) 261, 792, 1008
MaeIII GTNAC 2 cut(s) 176, 439
MalI GATC 3 cut(s) 204, 708, 804
MboI GATC 3 cut(s) 202, 706, 802
MboII GAAGA 3 cut(s) 438, 785, 963
MluCI AATT 3 cut(s) 689, 697, 1021
MlyI GAGTC 1 cut(s) 469
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 9 cut(s) 21, 44, 57, 200, 252, 357, 510, 829, 1006
Mph1103I ATGCAT 1 cut(s) 1087
MroXI GAANNNNTTC 1 cut(s) 681
MseI TTAA 1 cut(s) 74
MslI CAYNNNNRTG 2 cut(s) 594, 1112
MspA1I CMGCKG 2 cut(s) 48, 200
MspI CCGG 2 cut(s) 866, 1037
MspR9I CCNGG 3 cut(s) 490, 509, 866
Mva1269I GAATGC 3 cut(s) 97, 110, 1027
MvaI CCWGG 2 cut(s) 490, 509
MwoI GCNNNNNNNGC 2 cut(s) 504, 1033
NciI CCSGG 1 cut(s) 866
NcoI CCATGG 2 cut(s) 23, 849
NdeII GATC 3 cut(s) 202, 706, 802
NlaIII CATG 5 cut(s) 27, 64, 404, 853, 1158
NlaIV GGNNCC 3 cut(s) 32, 569, 928
NsiI ATGCAT 1 cut(s) 1087
PaeR7I CTCGAG 1 cut(s) 35
PagI TCATGA 1 cut(s) 1154
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 3 cut(s) 97, 110, 1027
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 4 cut(s) 316, 659, 869, 1148
PflFI GACNNNGTC 2 cut(s) 162, 267
PkrI GCNGC 3 cut(s) 21, 199, 948
PleI GAGTC 1 cut(s) 468
PpsI GAGTC 1 cut(s) 468
PpuMI RGGWCCY 1 cut(s) 31
Psp5II RGGWCCY 1 cut(s) 31
Psp6I CCWGG 2 cut(s) 488, 507
PspFI CCCAGC 1 cut(s) 1090
PspGI CCWGG 2 cut(s) 488, 507
PspN4I GGNNCC 3 cut(s) 32, 569, 928
PspPI GGNCC 2 cut(s) 31, 567
PspPPI RGGWCCY 1 cut(s) 31
PspXI VCTCGAGB 1 cut(s) 35
PstI CTGCAG 1 cut(s) 199
PsyI GACNNNGTC 2 cut(s) 162, 267
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 4 cut(s) 480, 790, 809, 922
RsaNI GTAC 4 cut(s) 479, 789, 808, 921
RseI CAYNNNNRTG 2 cut(s) 594, 1112
SaqAI TTAA 1 cut(s) 74
SatI GCNGC 3 cut(s) 20, 198, 947
Sau3AI GATC 3 cut(s) 202, 706, 802
Sau96I GGNCC 2 cut(s) 31, 567
ScaI AGTACT 1 cut(s) 809
SchI GAGTC 1 cut(s) 469
ScrFI CCNGG 3 cut(s) 490, 509, 866
SfaNI GCATC 4 cut(s) 280, 869, 977, 1116
SfcI CTRYAG 2 cut(s) 43, 195
Sfr274I CTCGAG 1 cut(s) 35
SinI GGWCC 2 cut(s) 31, 567
SlaI CTCGAG 1 cut(s) 35
SmiMI CAYNNNNRTG 2 cut(s) 594, 1112
SmlI CTYRAG 3 cut(s) 35, 320, 820
SmoI CTYRAG 3 cut(s) 35, 320, 820
Sse9I AATT 3 cut(s) 689, 697, 1021
SsiI CCGC 2 cut(s) 48, 83
SspI AATATT 1 cut(s) 1136
SspMI CTAG 3 cut(s) 261, 792, 1008
StyD4I CCNGG 3 cut(s) 488, 507, 864
StyI CCWWGG 4 cut(s) 23, 96, 798, 849
TaaI ACNGT 4 cut(s) 164, 525, 736, 1080
TaqI TCGA 4 cut(s) 36, 114, 207, 907
TasI AATT 3 cut(s) 689, 697, 1021
TatI WGTACW 2 cut(s) 788, 807
TfiI GAWTC 4 cut(s) 316, 659, 869, 1148
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 5 cut(s) 133, 199, 739, 981, 1119
TseI GCWGC 3 cut(s) 19, 197, 946
TspDTI ATGAA 5 cut(s) 92, 294, 442, 524, 786
TspRI CASTG 5 cut(s) 133, 199, 739, 981, 1119
Tth111I GACNNNGTC 2 cut(s) 162, 267
VpaK11BI GGWCC 2 cut(s) 31, 567
XapI RAATTY 1 cut(s) 689
XcmI CCANNNNNNNNNTGG 2 cut(s) 281, 757
XhoI CTCGAG 1 cut(s) 35
XmiI GTMKAC 1 cut(s) 861
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 3 cut(s) 261, 792, 1008
ZrmI AGTACT 1 cut(s) 809
Zsp2I ATGCAT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.