MD15G1120500.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003990
Physical Location & Seq
Reverse (-)
8671128 .. 8674717
3590 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1120500.v1.1.491

Sequence Viewer

Length: 1182 bp
ATGGCGAAGGGTTTAGAGCAGCAGGAACTGGAAGAGCAGCCATGGCGGGTCCTCGAGTTCTACAGCGGCATTGGCGGCATGAGGTACTCGCTCATGAAGGCAGGAGTGAACGCCGAAGTCGTGGAAGCGTTCGACATCAACGACACCGCCAATGACGTTTACGAGCACAATTTCGGCCACCGTCCCTATCAGGGTAATATTCAGAGTCTTACTGCTGCTGATCTCGACCGTTATCAGGCAGATGCATGGCTTCTTTCTCCTCCTTGCCAACCTTACACTCGACAAGGTCTTCAGAAGCAATCCGGTGATGCTCGGGCATTTTCATTTCTCAACATTCTTGAGTTGATACCACACACATCTCGACCTCCAATGATGTTATTTGTGGAAAATGTTGTCGGATTTGAGACATCTGATACGCATACAAAAATGATAGAGATATTGGGAAGAACAAATTTTGTTACACAGGAGTTCATATTGAGTCCGTTACAGTTTGATGTACCGTATTCCAGGCCACGCTATTTTTGCCTGGCAAAGAGGAAACCTTCAACCTTTCATAATCAAGTTTTCGATAATCAGCTTCTTCACTCGCTAGGGCCATTATTTGGGGATACAAATGCCGATGAGACAGTGCTCTGTGAACTTGATAAACCACAAAAGAGCCAGGATAAGTTACTTGAATCTTGTGAGCCTATAGAAAGCTTTCTTGAATTCAAGAATTGTAGTGACCAATCGGATTCCAATTTTGTGGACACCACTACTAGTTCTACAGATACTGATGGAATATTGGAGAAAGATGAAGGGAATGGATATTGTAGCGGCATCGTTGATCAGTACATTGTTCCATTAAGCTTGATAGAGAGGTGGGGAAGTGCCATGGATATTGTCTATCCTGATTCAAAACGATGTTGTTGTTTTACAAAAAGTTACCATCGATATGTGAAGGGTACAGGCTCCCTTTTGGCAACTCTGCAGCCAAAGAATAAGGATAAAACATCTTCATTGAAGGAGCAGCACCTTAGATACTTTTCCCCAAGGGAGGTTGCTAATTTGCATTCTCTCCCGGAAGAATTTCAGTTTCCACAACAGATAAGCCTCAGGCAGCGTTATGCATTACTGGGGAACAGTTTGAGTATAGCAGTGGTTGCTCCTTTACTCAAATACTTATTTGCCGGATCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

394

Amino Acids

44.53

Weight (kDa)

5.22

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 16 - 386 9.9e-36 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 602
AciI CCGC 5 cut(s) 46, 66, 75, 147, 816
AclWI GGATC 1 cut(s) 1180
AcoI YGGCCR 1 cut(s) 175
AcsI RAATTY 3 cut(s) 451, 707, 1067
AcuI CTGAAG 1 cut(s) 275
AfaI GTAC 4 cut(s) 86, 498, 833, 946
AfiI CCNNNNNNNGG 4 cut(s) 191, 235, 602, 1036
AgsI TTSAA 6 cut(s) 546, 677, 707, 712, 897, 1003
AhlI ACTAGT 1 cut(s) 758
AjnI CCWGG 3 cut(s) 506, 525, 660
AluBI AGCT 3 cut(s) 577, 699, 849
AluI AGCT 3 cut(s) 577, 699, 849
Alw21I GWGCWC 2 cut(s) 168, 633
Alw26I GTCTC 2 cut(s) 398, 617
AlwI GGATC 1 cut(s) 1180
AlwNI CAGNNNCTG 2 cut(s) 28, 773
Ama87I CYCGRG 2 cut(s) 53, 312
AoxI GGCC 3 cut(s) 175, 509, 593
ApeKI GCWGC 6 cut(s) 19, 37, 215, 970, 1009, 1099
ApoI RAATTY 3 cut(s) 451, 707, 1067
Asp700I GAANNNNTTC 2 cut(s) 699, 1068
AspS9I GGNCC 2 cut(s) 49, 593
AsuC2I CCSGG 1 cut(s) 1061
AsuHPI GGTGA 1 cut(s) 317
AvaI CYCGRG 2 cut(s) 53, 312
AvaII GGWCC 1 cut(s) 49
AxyI CCTNAGG 1 cut(s) 1094
BbsI GAAGAC 1 cut(s) 281
Bbv12I GWGCWC 2 cut(s) 168, 633
BbvI GCAGC 6 cut(s) 31, 49, 202, 982, 1021, 1111
BccI CCATC 2 cut(s) 770, 936
BciT130I CCWGG 3 cut(s) 508, 527, 662
BciVI GTATCC 1 cut(s) 601
BclI TGATCA 1 cut(s) 826
BcnI CCSGG 1 cut(s) 1061
BcoDI GTCTC 2 cut(s) 398, 617
BcuI ACTAGT 1 cut(s) 758
BfaI CTAG 2 cut(s) 590, 759
BfmI CTRYAG 4 cut(s) 61, 690, 765, 968
BfuI GTATCC 1 cut(s) 601
BisI GCNGC 9 cut(s) 20, 38, 67, 76, 216, 817, 971, 1010, 1100
BlsI GCNGC 9 cut(s) 21, 39, 68, 77, 217, 818, 972, 1011, 1101
Bme1390I CCNGG 4 cut(s) 508, 527, 662, 1061
Bme18I GGWCC 1 cut(s) 49
BmeT110I CYCGRG 2 cut(s) 53, 312
BmgT120I GGNCC 2 cut(s) 49, 593
BmiI GGNNCC 2 cut(s) 50, 952
BmrFI CCNGG 4 cut(s) 508, 527, 662, 1061
BmrI ACTGGG 1 cut(s) 1124
BmsI GCATC 3 cut(s) 232, 298, 828
BmuI ACTGGG 1 cut(s) 1124
BpiI GAAGAC 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 1 cut(s) 1061
Bsa29I ATCGAT 1 cut(s) 931
BsaJI CCNNGG 3 cut(s) 41, 873, 1031
BsaWI WCCGGW 1 cut(s) 302
Bsc4I CCNNNNNNNGG 4 cut(s) 191, 235, 602, 1036
Bse1I ACTGG 2 cut(s) 33, 1119
Bse21I CCTNAGG 1 cut(s) 1094
BseBI CCWGG 3 cut(s) 508, 527, 662
BseCI ATCGAT 1 cut(s) 931
BseDI CCNNGG 3 cut(s) 41, 873, 1031
BseLI CCNNNNNNNGG 4 cut(s) 191, 235, 602, 1036
BseMII CTCAG 1 cut(s) 1108
BseNI ACTGG 2 cut(s) 33, 1119
BseRI GAGGAG 1 cut(s) 249
BseXI GCAGC 6 cut(s) 31, 49, 202, 982, 1021, 1111
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 3 cut(s) 177, 511, 595
BshVI ATCGAT 1 cut(s) 931
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 2 cut(s) 168, 633
BsiHKCI CYCGRG 2 cut(s) 53, 312
BsiSI CCGG 3 cut(s) 303, 1061, 1170
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 4 cut(s) 191, 235, 602, 1036
BsmAI GTCTC 2 cut(s) 398, 617
BsmFI GGGAC 1 cut(s) 168
BsmI GAATGC 1 cut(s) 1051
BsnI GGCC 3 cut(s) 177, 511, 595
BsoBI CYCGRG 2 cut(s) 53, 312
Bsp1286I GDGCHC 2 cut(s) 168, 633
Bsp143I GATC 3 cut(s) 220, 826, 1172
Bsp19I CCATGG 2 cut(s) 41, 873
BspACI CCGC 5 cut(s) 46, 66, 75, 147, 816
BspANI GGCC 3 cut(s) 177, 511, 595
BspCNI CTCAG 1 cut(s) 1107
BspDI ATCGAT 1 cut(s) 931
BspHI TCATGA 2 cut(s) 93, 1178
BspLI GGNNCC 2 cut(s) 50, 952
BspMAI CTGCAG 1 cut(s) 972
BspPI GGATC 1 cut(s) 1180
BspQI GCTCTTC 1 cut(s) 27
BsrI ACTGG 2 cut(s) 33, 1119
BssECI CCNNGG 3 cut(s) 41, 873, 1031
BssMI GATC 3 cut(s) 220, 826, 1172
BssT1I CCWWGG 3 cut(s) 41, 873, 1031
Bst2UI CCWGG 3 cut(s) 508, 527, 662
Bst4CI ACNGT 6 cut(s) 182, 230, 489, 501, 628, 1124
Bst6I CTCTTC 1 cut(s) 27
BstAPI GCANNNNNTGC 1 cut(s) 1142
BstDEI CTNAG 2 cut(s) 1016, 1094
BstDSI CCRYGG 2 cut(s) 41, 873
BstKTI GATC 3 cut(s) 223, 829, 1175
BstMAI GTCTC 2 cut(s) 398, 617
BstMBI GATC 3 cut(s) 220, 826, 1172
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 5 cut(s) 43, 72, 75, 522, 1142
BstNI CCWGG 3 cut(s) 508, 527, 662
BstSCI CCNGG 4 cut(s) 506, 525, 660, 1059
BstSFI CTRYAG 4 cut(s) 61, 690, 765, 968
BstV1I GCAGC 6 cut(s) 31, 49, 202, 982, 1021, 1111
BstV2I GAAGAC 1 cut(s) 281
BstXI CCANNNNNNTGG 1 cut(s) 745
Bsu15I ATCGAT 1 cut(s) 931
Bsu36I CCTNAGG 1 cut(s) 1094
BsuI GTATCC 1 cut(s) 601
BsuRI GGCC 3 cut(s) 177, 511, 595
BsuTUI ATCGAT 1 cut(s) 931
BtgI CCRYGG 2 cut(s) 41, 873
BtsI GCAGTG 1 cut(s) 1143
BtsIMutI CAGTG 2 cut(s) 633, 1143
CaiI CAGNNNCTG 2 cut(s) 28, 773
CciI TCATGA 2 cut(s) 93, 1178
Cfr13I GGNCC 2 cut(s) 49, 593
ClaI ATCGAT 1 cut(s) 931
Csp6I GTAC 4 cut(s) 85, 497, 832, 945
CviAII CATG 6 cut(s) 42, 79, 94, 246, 874, 1179
CviQI GTAC 4 cut(s) 85, 497, 832, 945
DdeI CTNAG 2 cut(s) 1016, 1094
DpnI GATC 3 cut(s) 222, 828, 1174
DpnII GATC 3 cut(s) 220, 826, 1172
EaeI YGGCCR 1 cut(s) 175
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco130I CCWWGG 3 cut(s) 41, 873, 1031
Eco47I GGWCC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 275
Eco81I CCTNAGG 1 cut(s) 1094
Eco88I CYCGRG 2 cut(s) 53, 312
EcoO109I RGGNCCY 1 cut(s) 49
EcoRI GAATTC 1 cut(s) 707
EcoRII CCWGG 3 cut(s) 506, 525, 660
EcoT14I CCWWGG 3 cut(s) 41, 873, 1031
EcoT22I ATGCAT 2 cut(s) 247, 1111
ErhI CCWWGG 3 cut(s) 41, 873, 1031
FaeI CATG 6 cut(s) 45, 82, 97, 249, 877, 1182
FaqI GGGAC 1 cut(s) 168
FatI CATG 6 cut(s) 41, 78, 93, 245, 873, 1178
FauI CCCGC 1 cut(s) 39
FbaI TGATCA 1 cut(s) 826
Fnu4HI GCNGC 9 cut(s) 20, 38, 67, 76, 216, 817, 971, 1010, 1100
Fsp4HI GCNGC 9 cut(s) 20, 38, 67, 76, 216, 817, 971, 1010, 1100
FspBI CTAG 2 cut(s) 590, 759
GluI GCNGC 9 cut(s) 20, 38, 67, 76, 216, 817, 971, 1010, 1100
HaeIII GGCC 3 cut(s) 177, 511, 595
HapII CCGG 3 cut(s) 303, 1061, 1170
Hin1II CATG 6 cut(s) 45, 82, 97, 249, 877, 1182
HindIII AAGCTT 2 cut(s) 697, 847
HinfI GANTC 5 cut(s) 205, 478, 677, 734, 893
HpaII CCGG 3 cut(s) 303, 1061, 1170
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 4 cut(s) 109, 160, 638, 748
Hpy188I TCNGA 5 cut(s) 204, 294, 398, 412, 733
Hpy188III TCNNGA 8 cut(s) 94, 224, 338, 360, 704, 712, 890, 1179
Hpy8I GTNNAC 4 cut(s) 109, 160, 638, 748
HpyAV CCTTC 5 cut(s) 91, 552, 791, 934, 997
HpyCH4III ACNGT 6 cut(s) 182, 230, 489, 501, 628, 1124
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 4 cut(s) 245, 970, 1051, 1109
HpyF10VI GCNNNNNNNGC 5 cut(s) 43, 72, 75, 522, 1142
HpyF3I CTNAG 2 cut(s) 1016, 1094
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 6 cut(s) 45, 82, 97, 249, 877, 1182
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 3 cut(s) 220, 826, 1172
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 3 cut(s) 956, 1006, 1150
Lsp1109I GCAGC 6 cut(s) 31, 49, 202, 982, 1021, 1111
LweI GCATC 3 cut(s) 232, 298, 828
MaeI CTAG 2 cut(s) 590, 759
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 5 cut(s) 457, 483, 669, 722, 923
MalI GATC 3 cut(s) 222, 828, 1174
MboI GATC 3 cut(s) 220, 826, 1172
MboII GAAGA 6 cut(s) 44, 281, 456, 572, 987, 1076
MhlI GDGCHC 2 cut(s) 168, 633
MluCI AATT 7 cut(s) 169, 451, 707, 715, 739, 1045, 1067
MlyI GAGTC 2 cut(s) 214, 487
MmeI TCCRAC 1 cut(s) 376
MnlI CCTC 8 cut(s) 62, 75, 270, 375, 528, 852, 1030, 1103
Mph1103I ATGCAT 2 cut(s) 247, 1111
MroXI GAANNNNTTC 2 cut(s) 699, 1068
MseI TTAA 1 cut(s) 845
MslI CAYNNNNRTG 1 cut(s) 933
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 3 cut(s) 303, 1061, 1170
MspR9I CCNGG 4 cut(s) 508, 527, 662, 1061
Mva1269I GAATGC 1 cut(s) 1051
MvaI CCWGG 3 cut(s) 508, 527, 662
MwoI GCNNNNNNNGC 5 cut(s) 43, 72, 75, 522, 1142
NciI CCSGG 1 cut(s) 1061
NcoI CCATGG 2 cut(s) 41, 873
NdeII GATC 3 cut(s) 220, 826, 1172
NlaIII CATG 6 cut(s) 45, 82, 97, 249, 877, 1182
NlaIV GGNNCC 2 cut(s) 50, 952
NmuCI GTSAC 1 cut(s) 722
NsiI ATGCAT 2 cut(s) 247, 1111
PaeR7I CTCGAG 1 cut(s) 53
PagI TCATGA 2 cut(s) 93, 1178
PciSI GCTCTTC 1 cut(s) 27
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 138
PctI GAATGC 1 cut(s) 1051
PdmI GAANNNNTTC 2 cut(s) 699, 1068
PfeI GAWTC 3 cut(s) 677, 734, 893
PflFI GACNNNGTC 1 cut(s) 285
PflMI CCANNNNNTGG 1 cut(s) 602
PfoI TCCNGGA 1 cut(s) 1059
PkrI GCNGC 9 cut(s) 21, 39, 68, 77, 217, 818, 972, 1011, 1101
PleI GAGTC 2 cut(s) 213, 486
PpsI GAGTC 2 cut(s) 213, 486
PpuMI RGGWCCY 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 49
Psp6I CCWGG 3 cut(s) 506, 525, 660
PspGI CCWGG 3 cut(s) 506, 525, 660
PspN4I GGNNCC 2 cut(s) 50, 952
PspPI GGNCC 2 cut(s) 49, 593
PspPPI RGGWCCY 1 cut(s) 49
PspXI VCTCGAGB 1 cut(s) 53
PstI CTGCAG 1 cut(s) 972
PstNI CAGNNNCTG 2 cut(s) 28, 773
PsyI GACNNNGTC 1 cut(s) 285
RsaI GTAC 4 cut(s) 86, 498, 833, 946
RsaNI GTAC 4 cut(s) 85, 497, 832, 945
RseI CAYNNNNRTG 1 cut(s) 933
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 845
SatI GCNGC 9 cut(s) 20, 38, 67, 76, 216, 817, 971, 1010, 1100
Sau3AI GATC 3 cut(s) 220, 826, 1172
Sau96I GGNCC 2 cut(s) 49, 593
SchI GAGTC 2 cut(s) 214, 487
ScrFI CCNGG 4 cut(s) 508, 527, 662, 1061
SduI GDGCHC 2 cut(s) 168, 633
SfaNI GCATC 3 cut(s) 232, 298, 828
SfcI CTRYAG 4 cut(s) 61, 690, 765, 968
Sfr274I CTCGAG 1 cut(s) 53
SinI GGWCC 1 cut(s) 49
SlaI CTCGAG 1 cut(s) 53
SmiMI CAYNNNNRTG 1 cut(s) 933
SmlI CTYRAG 2 cut(s) 53, 338
SmoI CTYRAG 2 cut(s) 53, 338
SpeI ACTAGT 1 cut(s) 758
Sse9I AATT 7 cut(s) 169, 451, 707, 715, 739, 1045, 1067
SsiI CCGC 5 cut(s) 46, 66, 75, 147, 816
SspI AATATT 2 cut(s) 199, 783
SspMI CTAG 2 cut(s) 590, 759
StyD4I CCNGG 4 cut(s) 506, 525, 660, 1059
StyI CCWWGG 3 cut(s) 41, 873, 1031
TaaI ACNGT 6 cut(s) 182, 230, 489, 501, 628, 1124
TaiI ACGT 1 cut(s) 159
TaqI TCGA 7 cut(s) 54, 132, 225, 280, 361, 567, 931
TasI AATT 7 cut(s) 169, 451, 707, 715, 739, 1045, 1067
TatI WGTACW 1 cut(s) 831
TauI GCSGC 3 cut(s) 69, 78, 819
TfiI GAWTC 3 cut(s) 677, 734, 893
Tru1I TTAA 1 cut(s) 845
Tru9I TTAA 1 cut(s) 845
TscAI CASTG 2 cut(s) 633, 1143
TseFI GTSAC 1 cut(s) 722
TseI GCWGC 6 cut(s) 19, 37, 215, 970, 1009, 1099
Tsp45I GTSAC 1 cut(s) 722
TspDTI ATGAA 6 cut(s) 110, 312, 460, 542, 810, 987
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 2 cut(s) 633, 1143
Tth111I GACNNNGTC 1 cut(s) 285
Van91I CCANNNNNTGG 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 3 cut(s) 451, 707, 1067
XhoI CTCGAG 1 cut(s) 53
XmnI GAANNNNTTC 2 cut(s) 699, 1068
XspI CTAG 2 cut(s) 590, 759
Zsp2I ATGCAT 2 cut(s) 247, 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.