Rh6BG400900
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003990
Physical Location & Seq
Forward (+)
62811560 .. 62814853
3294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG400900.1

Sequence Viewer

Length: 909 bp
ATGGAGAAGGGTTCTGAGCAGCCATGGAGGGTCCTCGAGTTCTACAGCGGTATTGGTGGCATGAGGTATTCGTTAATGAAGGCGGGAGTGAATGCCAACGTTGTTGAAGCATTCGACATCAACGACACTGCCAACGATGTTTATCAGCACAACTTCGGACACCGTCCTTATCAGGGTAACATTCAGAGCCTTACTGCAGCTGATCTCGATAGATATGGAGCGAATGTATGGCTTCTGTCTCCTCCTTGCCAACCTTATACTAGACAAGGTCTCCAAAAGCAATCTGGTGATGCTCGGGCATTTTCATTTCTCCAGATTCTTGAGTTGATACCACACACCTCGCAACCTCCAATAATGTTATTTGTGGAAAATGTTGTTGGATTTGAGACATCTGATACACATGGAAAAATGATAGAGATATTGAGAAGAAGTGATTTTGTTACACAGGAGTTCATATTGAGTCCCTTGCAGTTTGGTGTACCATATTCCAGGCCACGCTATTTTTGCCTGGCAAAGAGGAAACCGTCAACCTTTCATAGTCAACTTTTCAATAATCATCTTCTTTGGTCCCCAAAGCCCTTATTTGGGCATACAAATGCGGATGACAAAGTGTTCTGTGAACTTGATGAGCCGCAAGGAAGCTTGGATAAGTTACTTGAATCTTGTGAGCCAATAGAAAGCTTTCTTGAATTCAAGAATTGCCGTGATCAGCCGAACTTTGTGGACAACACCACTGTTTCTACAGATACCATTGGAGTATTGGAGAAAGATGAAGAGAATGGGTGTTGTACTAGCACCTTGGATCAGTACTCTGTTCCCTCAAGCTTGATAGAGAGATGGGGAAGTGCCATGGGTATCCTTGTTTTACGTTTTATTTTTGTCATTGATTTGAACATAGGAATTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

302

Amino Acids

34.19

Weight (kDa)

5.03

Isoelectric Point (pI)

49.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 10 - 184 5.2e-24 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 48, 83, 599, 632
AclI AACGTT 1 cut(s) 99
AclWI GGATC 1 cut(s) 810
AcsI RAATTY 1 cut(s) 689
AfaI GTAC 3 cut(s) 480, 790, 809
AfiI CCNNNNNNNGG 3 cut(s) 173, 584, 585
AgsI TTSAA 6 cut(s) 107, 550, 659, 689, 694, 892
AjnI CCWGG 2 cut(s) 488, 507
AjuI GAANNNNNNNTTGG 2 cut(s) 360, 392
AluBI AGCT 4 cut(s) 200, 642, 681, 825
AluI AGCT 4 cut(s) 200, 642, 681, 825
Alw26I GTCTC 3 cut(s) 243, 275, 380
AlwI GGATC 1 cut(s) 810
Ama87I CYCGRG 2 cut(s) 35, 294
AoxI GGCC 1 cut(s) 491
ApeKI GCWGC 2 cut(s) 19, 197
ApoI RAATTY 1 cut(s) 689
Asp700I GAANNNNTTC 1 cut(s) 681
AspS9I GGNCC 2 cut(s) 31, 567
AsuHPI GGTGA 1 cut(s) 299
AvaI CYCGRG 2 cut(s) 35, 294
AvaII GGWCC 2 cut(s) 31, 567
BbvI GCAGC 2 cut(s) 31, 209
BccI CCATC 1 cut(s) 831
BceAI ACGGC 1 cut(s) 687
BciT130I CCWGG 2 cut(s) 490, 509
BciVI GTATCC 1 cut(s) 866
BclI TGATCA 1 cut(s) 706
BcoDI GTCTC 3 cut(s) 243, 275, 380
BfaI CTAG 2 cut(s) 261, 792
BfmI CTRYAG 3 cut(s) 43, 195, 741
BfuI GTATCC 1 cut(s) 866
BisI GCNGC 3 cut(s) 20, 198, 632
BlsI GCNGC 3 cut(s) 21, 199, 633
BmcAI AGTACT 1 cut(s) 809
Bme1390I CCNGG 2 cut(s) 490, 509
Bme18I GGWCC 2 cut(s) 31, 567
BmeT110I CYCGRG 2 cut(s) 35, 294
BmgT120I GGNCC 2 cut(s) 31, 567
BmiI GGNNCC 2 cut(s) 32, 569
BmrFI CCNGG 2 cut(s) 490, 509
BmsI GCATC 1 cut(s) 280
BpmI CTGGAG 1 cut(s) 296
BpuEI CTTGAG 2 cut(s) 341, 805
BsaBI GATNNNNATC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 3 cut(s) 23, 798, 849
BsaXI ACNNNNNCTCC 4 cut(s) 210, 240, 255, 285
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 584, 585
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 2 cut(s) 490, 509
BseDI CCNNGG 3 cut(s) 23, 798, 849
BseGI GGATG 1 cut(s) 607
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 3 cut(s) 173, 584, 585
BseMII CTCAG 1 cut(s) 6
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 2 cut(s) 31, 209
BshFI GGCC 1 cut(s) 493
BsiHKCI CYCGRG 2 cut(s) 35, 294
BslFI GGGAC 2 cut(s) 447, 553
BslI CCNNNNNNNGG 3 cut(s) 173, 584, 585
BsmAI GTCTC 3 cut(s) 243, 275, 380
BsmFI GGGAC 2 cut(s) 447, 553
BsmI GAATGC 2 cut(s) 97, 110
BsnI GGCC 1 cut(s) 493
Bso31I GGTCTC 1 cut(s) 275
BsoBI CYCGRG 2 cut(s) 35, 294
Bsp143I GATC 3 cut(s) 202, 706, 802
Bsp19I CCATGG 2 cut(s) 23, 849
BspACI CCGC 4 cut(s) 48, 83, 599, 632
BspANI GGCC 1 cut(s) 493
BspCNI CTCAG 1 cut(s) 7
BspLI GGNNCC 2 cut(s) 32, 569
BspMAI CTGCAG 1 cut(s) 199
BspPI GGATC 1 cut(s) 810
BspTNI GGTCTC 1 cut(s) 275
BssECI CCNNGG 3 cut(s) 23, 798, 849
BssMI GATC 3 cut(s) 202, 706, 802
BssT1I CCWWGG 3 cut(s) 23, 798, 849
Bst2UI CCWGG 2 cut(s) 490, 509
Bst4CI ACNGT 3 cut(s) 164, 525, 736
Bst6I CTCTTC 1 cut(s) 768
BstDEI CTNAG 1 cut(s) 15
BstDSI CCRYGG 2 cut(s) 23, 849
BstF5I GGATG 1 cut(s) 607
BstKTI GATC 3 cut(s) 205, 709, 805
BstMAI GTCTC 3 cut(s) 243, 275, 380
BstMBI GATC 3 cut(s) 202, 706, 802
BstMWI GCNNNNNNNGC 1 cut(s) 504
BstNI CCWGG 2 cut(s) 490, 509
BstSCI CCNGG 2 cut(s) 488, 507
BstSFI CTRYAG 3 cut(s) 43, 195, 741
BstV1I GCAGC 2 cut(s) 31, 209
BsuI GTATCC 1 cut(s) 866
BsuRI GGCC 1 cut(s) 493
BtgI CCRYGG 2 cut(s) 23, 849
BtsCI GGATG 1 cut(s) 607
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 2 cut(s) 126, 732
Cfr13I GGNCC 2 cut(s) 31, 567
Csp6I GTAC 3 cut(s) 479, 789, 808
CviAII CATG 5 cut(s) 24, 61, 401, 850, 906
CviQI GTAC 3 cut(s) 479, 789, 808
DdeI CTNAG 1 cut(s) 15
DpnI GATC 3 cut(s) 204, 708, 804
DpnII GATC 3 cut(s) 202, 706, 802
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Eco130I CCWWGG 3 cut(s) 23, 798, 849
Eco31I GGTCTC 1 cut(s) 275
Eco47I GGWCC 2 cut(s) 31, 567
Eco88I CYCGRG 2 cut(s) 35, 294
EcoO109I RGGNCCY 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 689
EcoRII CCWGG 2 cut(s) 488, 507
EcoT14I CCWWGG 3 cut(s) 23, 798, 849
ErhI CCWWGG 3 cut(s) 23, 798, 849
FaeI CATG 5 cut(s) 27, 64, 404, 853, 909
FaqI GGGAC 2 cut(s) 447, 553
FatI CATG 5 cut(s) 23, 60, 400, 849, 905
FauI CCCGC 1 cut(s) 76
FbaI TGATCA 1 cut(s) 706
Fnu4HI GCNGC 3 cut(s) 20, 198, 632
FokI GGATG 1 cut(s) 614
Fsp4HI GCNGC 3 cut(s) 20, 198, 632
FspBI CTAG 2 cut(s) 261, 792
GluI GCNGC 3 cut(s) 20, 198, 632
GsuI CTGGAG 1 cut(s) 296
HaeIII GGCC 1 cut(s) 493
Hin1II CATG 5 cut(s) 27, 64, 404, 853, 909
HincII GTYRAC 2 cut(s) 528, 542
HindII GTYRAC 2 cut(s) 528, 542
HindIII AAGCTT 3 cut(s) 640, 679, 823
HinfI GANTC 3 cut(s) 316, 460, 659
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 5 cut(s) 479, 528, 542, 620, 724
Hpy188I TCNGA 4 cut(s) 16, 158, 186, 394
Hpy188III TCNNGA 5 cut(s) 206, 313, 320, 686, 694
Hpy8I GTNNAC 5 cut(s) 479, 528, 542, 620, 724
HpyAV CCTTC 1 cut(s) 73
HpyCH4III ACNGT 3 cut(s) 164, 525, 736
HpyCH4IV ACGT 2 cut(s) 99, 868
HpyCH4V TGCA 2 cut(s) 197, 469
HpyF10VI GCNNNNNNNGC 1 cut(s) 504
HpyF3I CTNAG 1 cut(s) 15
HpySE526I ACGT 2 cut(s) 99, 868
Hsp92II CATG 5 cut(s) 27, 64, 404, 853, 909
Ksp22I TGATCA 1 cut(s) 706
Kzo9I GATC 3 cut(s) 202, 706, 802
LmnI GCTCC 1 cut(s) 218
LpnPI CCDG 8 cut(s) 158, 270, 326, 431, 475, 494, 502, 521
Lsp1109I GCAGC 2 cut(s) 31, 209
LweI GCATC 1 cut(s) 280
MaeI CTAG 2 cut(s) 261, 792
MaeII ACGT 2 cut(s) 99, 868
MaeIII GTNAC 3 cut(s) 176, 439, 651
MalI GATC 3 cut(s) 204, 708, 804
MboI GATC 3 cut(s) 202, 706, 802
MboII GAAGA 3 cut(s) 438, 551, 785
MluCI AATT 3 cut(s) 689, 697, 900
MlyI GAGTC 1 cut(s) 469
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 8 cut(s) 21, 44, 57, 252, 349, 357, 510, 829
MroXI GAANNNNTTC 1 cut(s) 681
MseI TTAA 1 cut(s) 74
MslI CAYNNNNRTG 1 cut(s) 594
MspA1I CMGCKG 2 cut(s) 48, 200
MspR9I CCNGG 2 cut(s) 490, 509
Mva1269I GAATGC 2 cut(s) 97, 110
MvaI CCWGG 2 cut(s) 490, 509
MwoI GCNNNNNNNGC 1 cut(s) 504
NcoI CCATGG 2 cut(s) 23, 849
NdeII GATC 3 cut(s) 202, 706, 802
NlaIII CATG 5 cut(s) 27, 64, 404, 853, 909
NlaIV GGNNCC 2 cut(s) 32, 569
PaeR7I CTCGAG 1 cut(s) 35
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 2 cut(s) 97, 110
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 2 cut(s) 316, 659
PflFI GACNNNGTC 2 cut(s) 162, 267
PkrI GCNGC 3 cut(s) 21, 199, 633
PleI GAGTC 1 cut(s) 468
PpsI GAGTC 1 cut(s) 468
PpuMI RGGWCCY 1 cut(s) 31
Psp1406I AACGTT 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 31
Psp6I CCWGG 2 cut(s) 488, 507
PspGI CCWGG 2 cut(s) 488, 507
PspN4I GGNNCC 2 cut(s) 32, 569
PspPI GGNCC 2 cut(s) 31, 567
PspPPI RGGWCCY 1 cut(s) 31
PspXI VCTCGAGB 1 cut(s) 35
PstI CTGCAG 1 cut(s) 199
PsyI GACNNNGTC 2 cut(s) 162, 267
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 3 cut(s) 480, 790, 809
RsaNI GTAC 3 cut(s) 479, 789, 808
RseI CAYNNNNRTG 1 cut(s) 594
SaqAI TTAA 1 cut(s) 74
SatI GCNGC 3 cut(s) 20, 198, 632
Sau3AI GATC 3 cut(s) 202, 706, 802
Sau96I GGNCC 2 cut(s) 31, 567
ScaI AGTACT 1 cut(s) 809
SchI GAGTC 1 cut(s) 469
ScrFI CCNGG 2 cut(s) 490, 509
SfaNI GCATC 1 cut(s) 280
SfcI CTRYAG 3 cut(s) 43, 195, 741
Sfr274I CTCGAG 1 cut(s) 35
SinI GGWCC 2 cut(s) 31, 567
SlaI CTCGAG 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 3 cut(s) 35, 320, 820
SmoI CTYRAG 3 cut(s) 35, 320, 820
Sse9I AATT 3 cut(s) 689, 697, 900
SsiI CCGC 4 cut(s) 48, 83, 599, 632
SspMI CTAG 2 cut(s) 261, 792
StyD4I CCNGG 2 cut(s) 488, 507
StyI CCWWGG 3 cut(s) 23, 798, 849
TaaI ACNGT 3 cut(s) 164, 525, 736
TaiI ACGT 2 cut(s) 102, 871
TaqI TCGA 3 cut(s) 36, 114, 207
TasI AATT 3 cut(s) 689, 697, 900
TatI WGTACW 2 cut(s) 788, 807
TauI GCSGC 1 cut(s) 634
TfiI GAWTC 2 cut(s) 316, 659
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 2 cut(s) 133, 739
TseI GCWGC 2 cut(s) 19, 197
TspDTI ATGAA 5 cut(s) 92, 294, 442, 524, 786
TspRI CASTG 2 cut(s) 133, 739
Tth111I GACNNNGTC 2 cut(s) 162, 267
VpaK11BI GGWCC 2 cut(s) 31, 567
XapI RAATTY 1 cut(s) 689
XcmI CCANNNNNNNNNTGG 2 cut(s) 281, 757
XhoI CTCGAG 1 cut(s) 35
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 2 cut(s) 261, 792
ZrmI AGTACT 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.