Prupe.1G473200_v2.0.a1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0003990
Physical Location & Seq
Reverse (-)
39326293 .. 39329439
3147 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G473200.3

Sequence Viewer

Length: 1170 bp
ATGGAAAAGGGTTTAGGCAAGCAGGGAGACGAGCCATGGAGGGTCCTCGAGTTCTACAGCGGTATTGGTGGCATGAGGTACTCGCTGATGAAGGCTGGAGTGAATGCCAAAGTTGTGGAAGCATTCGACATCAACGACACAGCTAACGATGTTTACCAGCACAATTTCGGCCACCGTCCCTATCAGGGTAATATCCAGAGCCTTACTGCTGCCGATCTCGACAGGTATGGAGCGGATGCATGGCTTCTTTCTCCTCCTTGTCAACCTTACACACGACAAGGTCTCCAAAAGCAATCTGGTGATGCTCGGGCTTTTTCATTTCTCAACATTCTTGAGTTGATACCACACACTTCGCAACCTCCAATAATGTTATTTGTGGAAAATGTTGTTGGATTTGAGACGTCTGATACGCATACGAAAATGATAGAGATATTGGGAAGAACTAATTTTATTACGCAGGAGTTCATTTTAAGTCCGTTACAGTTTGGTGTGCCATATTCCAGGCCACGCTATTTTTGCCTGGCAAAGAGGAAACCTTCAACCTTTCAGAGTCAACTTTTCAATAATCAGCTTCTTCAGTCCCCAGGGCCATTATTTAGGCATACAAATACTGTGGAGACACTGTTCTGTGAACTTGATGAACCACAAGAGAGCTGGGATAGGTTGCTTGAATCTTGTGAGTCTATAGAAAGCTTCCTTGAATTCAAGAATTGCAGTGACCAATCAGAGTCCAATTTTGTGGACACCACTACTGTATCTACAGATAGTGATGAAGTTTTGGATGAAGGGAATGAATATTGTAGCGGTGGCCTAGATCAGTACGCTGTTCCATTGAGCTTGATAGAGAGGTGGGGAAGTGCCATGGATATTGTCTATCCTGATTCAAAGCGATGCTGTTGTTTTACAAAAAGTTATTATCGATATGTGAAGGGTACAGGCTCCCTTTTAGCAACTCTGCAGCCAAATAACGAGGATAAAGCATCTTCACTGAAGGAGCAGTGCCTTAGATACTTTACTCCTAGGGAGGTTGCTAATTTGCATTCTCTCCCAGAAGATTTTCAGTTTCCACAGCAAATAAGCCTTCGGCAACGTTATGCATTGCTGGGGAACAGTTTGAGCATAGCAGTGGTTGCTCCTTTACTGCAATATTTATTTTCCGAATCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

390

Amino Acids

44.08

Weight (kDa)

4.91

Isoelectric Point (pI)

49.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 404
AccBSI CCGCTC 1 cut(s) 233
AciI CCGC 3 cut(s) 60, 233, 804
AclI AACGTT 1 cut(s) 1090
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 701
AcuI CTGAAG 2 cut(s) 560, 1010
AcyI GRCGYC 1 cut(s) 401
AfaI GTAC 3 cut(s) 80, 821, 934
AfiI CCNNNNNNNGG 1 cut(s) 185
AgsI TTSAA 6 cut(s) 540, 562, 671, 701, 706, 885
AjnI CCWGG 3 cut(s) 500, 519, 583
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AloI GAACNNNNNNTCC 2 cut(s) 608, 640
AluBI AGCT 5 cut(s) 143, 571, 654, 693, 837
AluI AGCT 5 cut(s) 143, 571, 654, 693, 837
Alw26I GTCTC 4 cut(s) 21, 287, 392, 611
Ama87I CYCGRG 2 cut(s) 47, 306
AoxI GGCC 4 cut(s) 169, 503, 587, 808
ApeKI GCWGC 2 cut(s) 209, 958
ApoI RAATTY 1 cut(s) 701
Asp700I GAANNNNTTC 1 cut(s) 1056
AspA2I CCTAGG 1 cut(s) 1019
AspS9I GGNCC 2 cut(s) 43, 587
AsuHPI GGTGA 1 cut(s) 311
AvaI CYCGRG 2 cut(s) 47, 306
AvaII GGWCC 1 cut(s) 43
AvrII CCTAGG 1 cut(s) 1019
BaeI ACNNNNGTAYC 2 cut(s) 738, 771
BbvI GCAGC 2 cut(s) 196, 970
BciT130I CCWGG 3 cut(s) 502, 521, 585
BcoDI GTCTC 4 cut(s) 21, 287, 392, 611
BfaI CTAG 2 cut(s) 812, 1020
BfmI CTRYAG 4 cut(s) 55, 684, 759, 956
BisI GCNGC 2 cut(s) 210, 959
BlnI CCTAGG 1 cut(s) 1019
BlsI GCNGC 2 cut(s) 211, 960
Bme1390I CCNGG 3 cut(s) 502, 521, 585
Bme18I GGWCC 1 cut(s) 43
BmeT110I CYCGRG 2 cut(s) 47, 306
BmgT120I GGNCC 2 cut(s) 43, 587
BmiI GGNNCC 2 cut(s) 44, 940
BmrFI CCNGG 3 cut(s) 502, 521, 585
BmsI GCATC 4 cut(s) 226, 292, 881, 989
BpmI CTGGAG 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 353
Bsa29I ATCGAT 1 cut(s) 919
BsaHI GRCGYC 1 cut(s) 401
BsaI GGTCTC 1 cut(s) 287
BsaJI CCNNGG 5 cut(s) 35, 583, 584, 861, 1019
BsaXI ACNNNNNCTCC 4 cut(s) 267, 297, 608, 638
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse3DI GCAATG 1 cut(s) 1097
BseBI CCWGG 3 cut(s) 502, 521, 585
BseCI ATCGAT 1 cut(s) 919
BseDI CCNNGG 5 cut(s) 35, 583, 584, 861, 1019
BseGI GGATG 2 cut(s) 241, 787
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMI GCAATG 1 cut(s) 1097
BseRI GAGGAG 1 cut(s) 243
BseXI GCAGC 2 cut(s) 196, 970
BseYI CCCAGC 2 cut(s) 654, 1102
BshFI GGCC 4 cut(s) 171, 505, 589, 810
BshVI ATCGAT 1 cut(s) 919
BsiHKCI CYCGRG 2 cut(s) 47, 306
BslFI GGGAC 2 cut(s) 162, 565
BslI CCNNNNNNNGG 1 cut(s) 185
BsmAI GTCTC 4 cut(s) 21, 287, 392, 611
BsmBI CGTCTC 2 cut(s) 21, 392
BsmFI GGGAC 2 cut(s) 162, 565
BsmI GAATGC 3 cut(s) 109, 122, 1039
BsnI GGCC 4 cut(s) 171, 505, 589, 810
Bso31I GGTCTC 1 cut(s) 287
BsoBI CYCGRG 2 cut(s) 47, 306
Bsp143I GATC 2 cut(s) 214, 814
Bsp19I CCATGG 2 cut(s) 35, 861
BspACI CCGC 3 cut(s) 60, 233, 804
BspANI GGCC 4 cut(s) 171, 505, 589, 810
BspDI ATCGAT 1 cut(s) 919
BspHI TCATGA 1 cut(s) 1166
BspLI GGNNCC 2 cut(s) 44, 940
BspMAI CTGCAG 1 cut(s) 960
BspTNI GGTCTC 1 cut(s) 287
BsrBI CCGCTC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 1097
BssECI CCNNGG 5 cut(s) 35, 583, 584, 861, 1019
BssMI GATC 2 cut(s) 214, 814
BssNI GRCGYC 1 cut(s) 401
BssT1I CCWWGG 3 cut(s) 35, 861, 1019
Bst2UI CCWGG 3 cut(s) 502, 521, 585
Bst4CI ACNGT 6 cut(s) 176, 483, 613, 624, 754, 1112
BstACI GRCGYC 1 cut(s) 401
BstAPI GCANNNNNTGC 1 cut(s) 1130
BstC8I GCNNGC 1 cut(s) 20
BstDEI CTNAG 1 cut(s) 1004
BstDSI CCRYGG 2 cut(s) 35, 861
BstF5I GGATG 2 cut(s) 241, 787
BstKTI GATC 2 cut(s) 217, 817
BstMAI GTCTC 4 cut(s) 21, 287, 392, 611
BstMBI GATC 2 cut(s) 214, 814
BstMWI GCNNNNNNNGC 2 cut(s) 516, 1130
BstNI CCWGG 3 cut(s) 502, 521, 585
BstSCI CCNGG 3 cut(s) 500, 519, 583
BstSFI CTRYAG 4 cut(s) 55, 684, 759, 956
BstV1I GCAGC 2 cut(s) 196, 970
BstXI CCANNNNNNTGG 2 cut(s) 115, 739
Bsu15I ATCGAT 1 cut(s) 919
BsuRI GGCC 4 cut(s) 171, 505, 589, 810
BsuTUI ATCGAT 1 cut(s) 919
BtgI CCRYGG 2 cut(s) 35, 861
BtgZI GCGATG 1 cut(s) 904
BtsCI GGATG 2 cut(s) 241, 787
BtsI GCAGTG 3 cut(s) 721, 1004, 1131
BtsIMutI CAGTG 5 cut(s) 620, 721, 986, 1004, 1131
Cac8I GCNNGC 1 cut(s) 20
CciI TCATGA 1 cut(s) 1166
Cfr13I GGNCC 2 cut(s) 43, 587
ClaI ATCGAT 1 cut(s) 919
Csp6I GTAC 3 cut(s) 79, 820, 933
CspCI CAANNNNNGTGG 2 cut(s) 594, 629
CviAII CATG 5 cut(s) 36, 73, 240, 862, 1167
CviQI GTAC 3 cut(s) 79, 820, 933
DdeI CTNAG 1 cut(s) 1004
DpnI GATC 2 cut(s) 216, 816
DpnII GATC 2 cut(s) 214, 814
EaeI YGGCCR 1 cut(s) 169
Eco130I CCWWGG 3 cut(s) 35, 861, 1019
Eco31I GGTCTC 1 cut(s) 287
Eco47I GGWCC 1 cut(s) 43
Eco57I CTGAAG 2 cut(s) 560, 1010
Eco88I CYCGRG 2 cut(s) 47, 306
EcoO109I RGGNCCY 1 cut(s) 43
EcoRI GAATTC 1 cut(s) 701
EcoRII CCWGG 3 cut(s) 500, 519, 583
EcoT14I CCWWGG 3 cut(s) 35, 861, 1019
EcoT22I ATGCAT 2 cut(s) 241, 1099
ErhI CCWWGG 3 cut(s) 35, 861, 1019
Esp3I CGTCTC 2 cut(s) 21, 392
FaeI CATG 5 cut(s) 39, 76, 243, 865, 1170
FaqI GGGAC 2 cut(s) 162, 565
FatI CATG 5 cut(s) 35, 72, 239, 861, 1166
Fnu4HI GCNGC 2 cut(s) 210, 959
FokI GGATG 2 cut(s) 248, 794
Fsp4HI GCNGC 2 cut(s) 210, 959
FspBI CTAG 2 cut(s) 812, 1020
GluI GCNGC 2 cut(s) 210, 959
GsaI CCCAGC 2 cut(s) 658, 1106
GsuI CTGGAG 1 cut(s) 117
HaeIII GGCC 4 cut(s) 171, 505, 589, 810
Hin1I GRCGYC 1 cut(s) 401
Hin1II CATG 5 cut(s) 39, 76, 243, 865, 1170
HincII GTYRAC 2 cut(s) 263, 554
HindII GTYRAC 2 cut(s) 263, 554
HindIII AAGCTT 1 cut(s) 691
HinfI GANTC 6 cut(s) 550, 671, 680, 728, 881, 1160
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 5 cut(s) 154, 263, 554, 632, 742
Hpy188I TCNGA 4 cut(s) 406, 549, 727, 1159
Hpy188III TCNNGA 6 cut(s) 196, 218, 332, 706, 878, 1167
Hpy8I GTNNAC 5 cut(s) 154, 263, 554, 632, 742
HpyAV CCTTC 6 cut(s) 85, 546, 779, 922, 985, 1091
HpyCH4III ACNGT 6 cut(s) 176, 483, 613, 624, 754, 1112
HpyCH4IV ACGT 2 cut(s) 401, 1090
HpyCH4V TGCA 6 cut(s) 239, 714, 958, 1039, 1097, 1144
HpyF10VI GCNNNNNNNGC 2 cut(s) 516, 1130
HpyF3I CTNAG 1 cut(s) 1004
HpySE526I ACGT 2 cut(s) 401, 1090
Hsp92I GRCGYC 1 cut(s) 401
Hsp92II CATG 5 cut(s) 39, 76, 243, 865, 1170
Kzo9I GATC 2 cut(s) 214, 814
LmnI GCTCC 4 cut(s) 230, 944, 994, 1138
Lsp1109I GCAGC 2 cut(s) 196, 970
LweI GCATC 4 cut(s) 226, 292, 881, 989
MaeI CTAG 2 cut(s) 812, 1020
MaeII ACGT 2 cut(s) 401, 1090
MaeIII GTNAC 2 cut(s) 477, 716
MalI GATC 2 cut(s) 216, 816
MbiI CCGCTC 1 cut(s) 233
MboI GATC 2 cut(s) 214, 814
MboII GAAGA 4 cut(s) 450, 566, 975, 1064
MluCI AATT 6 cut(s) 163, 445, 701, 709, 733, 1033
MlyI GAGTC 3 cut(s) 559, 689, 737
MmeI TCCRAC 1 cut(s) 370
MnlI CCTC 9 cut(s) 33, 56, 69, 264, 369, 522, 840, 964, 1018
Mph1103I ATGCAT 2 cut(s) 241, 1099
MroXI GAANNNNTTC 1 cut(s) 1056
MseI TTAA 1 cut(s) 470
MslI CAYNNNNRTG 1 cut(s) 1124
MspA1I CMGCKG 1 cut(s) 60
MspR9I CCNGG 3 cut(s) 502, 521, 585
Mva1269I GAATGC 3 cut(s) 109, 122, 1039
MvaI CCWGG 3 cut(s) 502, 521, 585
MwoI GCNNNNNNNGC 2 cut(s) 516, 1130
NcoI CCATGG 2 cut(s) 35, 861
NdeII GATC 2 cut(s) 214, 814
NlaIII CATG 5 cut(s) 39, 76, 243, 865, 1170
NlaIV GGNNCC 2 cut(s) 44, 940
NmuCI GTSAC 1 cut(s) 716
NsiI ATGCAT 2 cut(s) 241, 1099
PaeR7I CTCGAG 1 cut(s) 47
PagI TCATGA 1 cut(s) 1166
PasI CCCWGGG 1 cut(s) 584
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PctI GAATGC 3 cut(s) 109, 122, 1039
PdmI GAANNNNTTC 1 cut(s) 1056
PfeI GAWTC 3 cut(s) 671, 881, 1160
PflFI GACNNNGTC 1 cut(s) 279
PkrI GCNGC 2 cut(s) 211, 960
PleI GAGTC 3 cut(s) 558, 688, 736
PpsI GAGTC 3 cut(s) 558, 688, 736
PpuMI RGGWCCY 1 cut(s) 43
Psp1406I AACGTT 1 cut(s) 1090
Psp5II RGGWCCY 1 cut(s) 43
Psp6I CCWGG 3 cut(s) 500, 519, 583
PspFI CCCAGC 2 cut(s) 654, 1102
PspGI CCWGG 3 cut(s) 500, 519, 583
PspN4I GGNNCC 2 cut(s) 44, 940
PspPI GGNCC 2 cut(s) 43, 587
PspPPI RGGWCCY 1 cut(s) 43
PspXI VCTCGAGB 1 cut(s) 47
PstI CTGCAG 1 cut(s) 960
PsyI GACNNNGTC 1 cut(s) 279
RsaI GTAC 3 cut(s) 80, 821, 934
RsaNI GTAC 3 cut(s) 79, 820, 933
RseI CAYNNNNRTG 1 cut(s) 1124
SaqAI TTAA 1 cut(s) 470
SatI GCNGC 2 cut(s) 210, 959
Sau3AI GATC 2 cut(s) 214, 814
Sau96I GGNCC 2 cut(s) 43, 587
SchI GAGTC 3 cut(s) 559, 689, 737
ScrFI CCNGG 3 cut(s) 502, 521, 585
SfaNI GCATC 4 cut(s) 226, 292, 881, 989
SfcI CTRYAG 4 cut(s) 55, 684, 759, 956
Sfr274I CTCGAG 1 cut(s) 47
SinI GGWCC 1 cut(s) 43
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 1124
SmlI CTYRAG 2 cut(s) 47, 332
SmoI CTYRAG 2 cut(s) 47, 332
Sse9I AATT 6 cut(s) 163, 445, 701, 709, 733, 1033
SsiI CCGC 3 cut(s) 60, 233, 804
SspI AATATT 2 cut(s) 797, 1148
SspMI CTAG 2 cut(s) 812, 1020
StyD4I CCNGG 3 cut(s) 500, 519, 583
StyI CCWWGG 3 cut(s) 35, 861, 1019
TaaI ACNGT 6 cut(s) 176, 483, 613, 624, 754, 1112
TaiI ACGT 2 cut(s) 404, 1093
TaqI TCGA 4 cut(s) 48, 126, 219, 919
TasI AATT 6 cut(s) 163, 445, 701, 709, 733, 1033
TfiI GAWTC 3 cut(s) 671, 881, 1160
Tru1I TTAA 1 cut(s) 470
Tru9I TTAA 1 cut(s) 470
TscAI CASTG 5 cut(s) 627, 721, 993, 1004, 1131
TseFI GTSAC 1 cut(s) 716
TseI GCWGC 2 cut(s) 209, 958
Tsp45I GTSAC 1 cut(s) 716
TspDTI ATGAA 7 cut(s) 104, 306, 454, 654, 786, 798, 807
TspGWI ACGGA 1 cut(s) 465
TspRI CASTG 5 cut(s) 627, 721, 993, 1004, 1131
Tth111I GACNNNGTC 1 cut(s) 279
VpaK11BI GGWCC 1 cut(s) 43
XapI RAATTY 1 cut(s) 701
XcmI CCANNNNNNNNNTGG 2 cut(s) 293, 651
XhoI CTCGAG 1 cut(s) 47
XmaJI CCTAGG 1 cut(s) 1019
XmnI GAANNNNTTC 1 cut(s) 1056
XspI CTAG 2 cut(s) 812, 1020
ZraI GACGTC 1 cut(s) 402
Zsp2I ATGCAT 2 cut(s) 241, 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.