FvH4_2g29650
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003990
Physical Location & Seq
Forward (+)
22954850 .. 22957889
3040 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29650.t1

Sequence Viewer

Length: 1233 bp
ATGACTATTAACCATAGCGGGTTAGTGCTCTTTTTCAACACATTGGAGACGAAACGCATGTCGTTTGTGTTTGAAAGCTTAATGGAGAAAGGTTGTGAGCAGCCATGGAGGGTCCTCGAGTTCTACAGCGGTATTGGTGGCATGAGGTATTCGTTAATGAAGGCGGGAGTGAATGCCAACGTTGTCGAAGCATTCGACATCAACGACACTGCCAACGATGTTTATCAGCACAACTTCGGACATCGTCCCTATCAGGGAAACATTCAGAGCCTTACTGCTGCTGATCTCGATAGATATAACGCGAATGTATGGCTTCTGTCTCCTCCTTGCCAACCTTACACTAGACAAGGTCTCCAAAAGCAATCTGGTGATGCTCGGGCATTTTCATTTCTCCAGATTCTTGAGTTGATACCACACACTTTGCAACCTCCAGTAATGTTATTTGTGGAAAATGTTGTTGGATTTGAGACATCTGATACACATGCAAAAATGTTAGAGATATTGAGAACAAGTGACTATGTTACACAGGAGTTCATATTGAGTCCCTTGCAGTTTGGTGTACCATATTCCAGGCCGCGCTATTTTTGCCTGGCAAAGAGGAAACCCTCGACCTTTCAGAGTCAACTTTTCGATAATCAGCTTCTTCGGTCGCCAAAGCCATTATATACAAATGCGGATGACAAAGTGTTCTGTGAACTTGATGAGCTGGAAGGGAGCTCAGACAAGTTGGTTGAATCTTGTCAGCCAATAGAAAGCTTTCTTGAATTCAAGAATTGCAGTGATTGCCCCAACTTTGTGGATGGCACTACTGTTTCTACAGATACCATTAGAGTTTTGGGCAAAGATGAAGAGAATGGTTGTTATACTAGCACATTGGATCAGTACACTGTTCCCTCAAGCTTGATAGAGAGATGGGGAAGTGCCATGGATATTGTCTACCCGGATTCAAAGAGATGTTGTTGTTTTACAAAAAGTTATTATCGATATGTGAAGGGAACTGGCTCCTTTTTGGCAACTGGGCAGGCAAAGATAAAGGATAAAGCATCTTCACTGAAGGAACAGAGCCTTAGATACTTCACCCCTAGGGAGGTTGCTAATTTGCATTCTCTGCCGGAAAGCTTTCAGTTTCCGCAGCACATAAGCCTTAGACAACGGTATGCATTGCTGGGAAACAGCTTGAGCATATCAGTGGTTGCTCCCTTACTCCGATATTTAATCGCTGAATCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

411

Amino Acids

46.54

Weight (kDa)

5.88

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 37 - 402 1.7e-35 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 936
AccII CGCG 2 cut(s) 302, 577
AciI CCGC 6 cut(s) 18, 129, 164, 575, 674, 1130
AclI AACGTT 1 cut(s) 180
AclWI GGATC 1 cut(s) 885
AcsI RAATTY 1 cut(s) 764
AcuI CTGAAG 1 cut(s) 1073
AfaI GTAC 2 cut(s) 561, 884
AfiI CCNNNNNNNGG 2 cut(s) 254, 1087
AgsI TTSAA 6 cut(s) 37, 74, 734, 764, 769, 948
AjnI CCWGG 2 cut(s) 569, 588
AjuI GAANNNNNNNTTGG 2 cut(s) 441, 473
AluBI AGCT 8 cut(s) 78, 640, 706, 717, 756, 900, 1119, 1176
AluI AGCT 8 cut(s) 78, 640, 706, 717, 756, 900, 1119, 1176
Alw21I GWGCWC 2 cut(s) 30, 719
Alw26I GTCTC 4 cut(s) 41, 324, 356, 461
AlwI GGATC 1 cut(s) 885
Ama87I CYCGRG 2 cut(s) 116, 375
AoxI GGCC 1 cut(s) 572
ApeKI GCWGC 3 cut(s) 100, 278, 1132
ApoI RAATTY 1 cut(s) 764
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
Asp700I GAANNNNTTC 2 cut(s) 756, 1119
AspA2I CCTAGG 1 cut(s) 1082
AspLEI GCGC 1 cut(s) 579
AspS9I GGNCC 1 cut(s) 112
AsuC2I CCSGG 1 cut(s) 941
AsuHPI GGTGA 2 cut(s) 380, 1069
AvaI CYCGRG 2 cut(s) 116, 375
AvaII GGWCC 1 cut(s) 112
AvrII CCTAGG 1 cut(s) 1082
BanII GRGCYC 1 cut(s) 719
Bbv12I GWGCWC 2 cut(s) 30, 719
BbvI GCAGC 3 cut(s) 112, 265, 1144
BccI CCATC 2 cut(s) 794, 906
BciT130I CCWGG 2 cut(s) 571, 590
BcnI CCSGG 1 cut(s) 941
BcoDI GTCTC 4 cut(s) 41, 324, 356, 461
BfaI CTAG 3 cut(s) 342, 867, 1083
BfmI CTRYAG 2 cut(s) 124, 816
BisI GCNGC 4 cut(s) 101, 279, 575, 1133
BlnI CCTAGG 1 cut(s) 1082
BlsI GCNGC 4 cut(s) 102, 280, 576, 1134
Bme1390I CCNGG 3 cut(s) 571, 590, 941
Bme18I GGWCC 1 cut(s) 112
BmeT110I CYCGRG 2 cut(s) 116, 375
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 2 cut(s) 113, 1003
BmrFI CCNGG 3 cut(s) 571, 590, 941
BmrI ACTGGG 1 cut(s) 1026
BmsI GCATC 2 cut(s) 361, 1052
BmuI ACTGGG 1 cut(s) 1026
BpmI CTGGAG 2 cut(s) 377, 414
BpuEI CTTGAG 3 cut(s) 422, 880, 1198
BpuMI CCSGG 1 cut(s) 941
Bsa29I ATCGAT 1 cut(s) 982
BsaBI GATNNNNATC 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 3 cut(s) 104, 924, 1082
BsaXI ACNNNNNCTCC 2 cut(s) 336, 366
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 1087
Bse1I ACTGG 3 cut(s) 431, 1003, 1021
Bse3DI GCAATG 1 cut(s) 1160
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 2 cut(s) 571, 590
BseCI ATCGAT 1 cut(s) 982
BseDI CCNNGG 3 cut(s) 104, 924, 1082
BseGI GGATG 2 cut(s) 682, 805
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 2 cut(s) 254, 1087
BseMI GCAATG 1 cut(s) 1160
BseMII CTCAG 1 cut(s) 732
BseNI ACTGG 3 cut(s) 431, 1003, 1021
BseRI GAGGAG 1 cut(s) 312
BseXI GCAGC 3 cut(s) 112, 265, 1144
BseYI CCCAGC 1 cut(s) 1165
Bsh1236I CGCG 2 cut(s) 302, 577
Bsh1285I CGRYCG 1 cut(s) 650
BshFI GGCC 1 cut(s) 574
BshVI ATCGAT 1 cut(s) 982
BsiEI CGRYCG 1 cut(s) 650
BsiHKAI GWGCWC 2 cut(s) 30, 719
BsiHKCI CYCGRG 2 cut(s) 116, 375
BsiSI CCGG 2 cut(s) 941, 1112
BslFI GGGAC 2 cut(s) 231, 528
BslI CCNNNNNNNGG 2 cut(s) 254, 1087
BsmAI GTCTC 4 cut(s) 41, 324, 356, 461
BsmBI CGTCTC 1 cut(s) 41
BsmFI GGGAC 2 cut(s) 231, 528
BsmI GAATGC 3 cut(s) 178, 191, 1102
BsnI GGCC 1 cut(s) 574
Bso31I GGTCTC 1 cut(s) 356
BsoBI CYCGRG 2 cut(s) 116, 375
Bsp1286I GDGCHC 2 cut(s) 30, 719
Bsp143I GATC 2 cut(s) 283, 877
Bsp19I CCATGG 2 cut(s) 104, 924
BspACI CCGC 6 cut(s) 18, 129, 164, 575, 674, 1130
BspANI GGCC 1 cut(s) 574
BspCNI CTCAG 1 cut(s) 731
BspDI ATCGAT 1 cut(s) 982
BspFNI CGCG 2 cut(s) 302, 577
BspHI TCATGA 1 cut(s) 1229
BspLI GGNNCC 2 cut(s) 113, 1003
BspPI GGATC 1 cut(s) 885
BspTNI GGTCTC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 1160
BsrI ACTGG 3 cut(s) 431, 1003, 1021
BssECI CCNNGG 3 cut(s) 104, 924, 1082
BssMI GATC 2 cut(s) 283, 877
BssT1I CCWWGG 3 cut(s) 104, 924, 1082
Bst2UI CCWGG 2 cut(s) 571, 590
Bst4CI ACNGT 3 cut(s) 811, 889, 1155
Bst6I CTCTTC 1 cut(s) 843
BstAPI GCANNNNNTGC 2 cut(s) 783, 1108
BstC8I GCNNGC 1 cut(s) 1023
BstDEI CTNAG 3 cut(s) 718, 1067, 1145
BstDSI CCRYGG 2 cut(s) 104, 924
BstF5I GGATG 2 cut(s) 682, 805
BstFNI CGCG 2 cut(s) 302, 577
BstHHI GCGC 1 cut(s) 579
BstKTI GATC 2 cut(s) 286, 880
BstMAI GTCTC 4 cut(s) 41, 324, 356, 461
BstMBI GATC 2 cut(s) 283, 877
BstMCI CGRYCG 1 cut(s) 650
BstMWI GCNNNNNNNGC 3 cut(s) 585, 783, 1108
BstNI CCWGG 2 cut(s) 571, 590
BstNSI RCATGY 2 cut(s) 61, 485
BstSCI CCNGG 3 cut(s) 569, 588, 939
BstSFI CTRYAG 2 cut(s) 124, 816
BstUI CGCG 2 cut(s) 302, 577
BstV1I GCAGC 3 cut(s) 112, 265, 1144
BstXI CCANNNNNNTGG 1 cut(s) 796
Bsu15I ATCGAT 1 cut(s) 982
BsuRI GGCC 1 cut(s) 574
BsuTUI ATCGAT 1 cut(s) 982
BtgI CCRYGG 2 cut(s) 104, 924
BtsCI GGATG 2 cut(s) 682, 805
BtsI GCAGTG 2 cut(s) 207, 784
BtsIMutI CAGTG 5 cut(s) 207, 784, 885, 1049, 1194
Cac8I GCNNGC 1 cut(s) 1023
CciI TCATGA 1 cut(s) 1229
CfoI GCGC 1 cut(s) 579
Cfr13I GGNCC 1 cut(s) 112
ClaI ATCGAT 1 cut(s) 982
Csp6I GTAC 2 cut(s) 560, 883
CspCI CAANNNNNGTGG 2 cut(s) 402, 437
CviAII CATG 6 cut(s) 58, 105, 142, 482, 925, 1230
CviQI GTAC 2 cut(s) 560, 883
DdeI CTNAG 3 cut(s) 718, 1067, 1145
DpnI GATC 2 cut(s) 285, 879
DpnII GATC 2 cut(s) 283, 877
Eam1104I CTCTTC 1 cut(s) 843
EarI CTCTTC 1 cut(s) 843
Ecl136II GAGCTC 1 cut(s) 717
Eco130I CCWWGG 3 cut(s) 104, 924, 1082
Eco24I GRGCYC 1 cut(s) 719
Eco31I GGTCTC 1 cut(s) 356
Eco47I GGWCC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 717
Eco57I CTGAAG 1 cut(s) 1073
Eco88I CYCGRG 2 cut(s) 116, 375
EcoICRI GAGCTC 1 cut(s) 717
EcoO109I RGGNCCY 1 cut(s) 112
EcoRI GAATTC 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 569, 588
EcoT14I CCWWGG 3 cut(s) 104, 924, 1082
EcoT22I ATGCAT 1 cut(s) 1162
EcoT38I GRGCYC 1 cut(s) 719
ErhI CCWWGG 3 cut(s) 104, 924, 1082
Esp3I CGTCTC 1 cut(s) 41
FaeI CATG 6 cut(s) 61, 108, 145, 485, 928, 1233
FaqI GGGAC 2 cut(s) 231, 528
FatI CATG 6 cut(s) 57, 104, 141, 481, 924, 1229
FauI CCCGC 2 cut(s) 11, 157
FblI GTMKAC 1 cut(s) 936
Fnu4HI GCNGC 4 cut(s) 101, 279, 575, 1133
FokI GGATG 2 cut(s) 689, 812
FriOI GRGCYC 1 cut(s) 719
Fsp4HI GCNGC 4 cut(s) 101, 279, 575, 1133
FspBI CTAG 3 cut(s) 342, 867, 1083
GlaI GCGC 1 cut(s) 578
GluI GCNGC 4 cut(s) 101, 279, 575, 1133
GsaI CCCAGC 1 cut(s) 1169
GsuI CTGGAG 2 cut(s) 377, 414
HaeIII GGCC 1 cut(s) 574
HapII CCGG 2 cut(s) 941, 1112
HhaI GCGC 1 cut(s) 579
Hin1II CATG 6 cut(s) 61, 108, 145, 485, 928, 1233
Hin6I GCGC 1 cut(s) 577
HinP1I GCGC 1 cut(s) 577
HincII GTYRAC 1 cut(s) 623
HindII GTYRAC 1 cut(s) 623
HindIII AAGCTT 4 cut(s) 76, 754, 898, 1117
HinfI GANTC 6 cut(s) 397, 541, 619, 734, 944, 1223
HpaII CCGG 2 cut(s) 941, 1112
HphI GGTGA 2 cut(s) 380, 1069
Hpy166II GTNNAC 5 cut(s) 560, 623, 695, 885, 937
Hpy188I TCNGA 6 cut(s) 239, 267, 475, 618, 721, 1208
Hpy188III TCNNGA 6 cut(s) 287, 394, 401, 761, 769, 1230
Hpy8I GTNNAC 5 cut(s) 560, 623, 695, 885, 937
HpyAV CCTTC 4 cut(s) 154, 704, 985, 1048
HpyCH4III ACNGT 3 cut(s) 811, 889, 1155
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 6 cut(s) 424, 485, 550, 777, 1102, 1160
HpyF10VI GCNNNNNNNGC 3 cut(s) 585, 783, 1108
HpyF3I CTNAG 3 cut(s) 718, 1067, 1145
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 6 cut(s) 61, 108, 145, 485, 928, 1233
HspAI GCGC 1 cut(s) 577
Kzo9I GATC 2 cut(s) 283, 877
LmnI GCTCC 3 cut(s) 714, 1007, 1201
Lsp1109I GCAGC 3 cut(s) 112, 265, 1144
LweI GCATC 2 cut(s) 361, 1052
MaeI CTAG 3 cut(s) 342, 867, 1083
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 2 cut(s) 512, 520
MalI GATC 2 cut(s) 285, 879
MboI GATC 2 cut(s) 283, 877
MboII GAAGA 3 cut(s) 635, 860, 1038
MhlI GDGCHC 2 cut(s) 30, 719
MluCI AATT 3 cut(s) 764, 772, 1096
MlyI GAGTC 2 cut(s) 550, 628
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 9 cut(s) 102, 125, 138, 333, 438, 591, 616, 904, 1081
Mph1103I ATGCAT 1 cut(s) 1162
MroXI GAANNNNTTC 2 cut(s) 756, 1119
MseI TTAA 4 cut(s) 9, 80, 155, 1214
MslI CAYNNNNRTG 1 cut(s) 1187
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 2 cut(s) 941, 1112
MspR9I CCNGG 3 cut(s) 571, 590, 941
Mva1269I GAATGC 3 cut(s) 178, 191, 1102
MvaI CCWGG 2 cut(s) 571, 590
MvnI CGCG 2 cut(s) 302, 577
MwoI GCNNNNNNNGC 3 cut(s) 585, 783, 1108
NciI CCSGG 1 cut(s) 941
NcoI CCATGG 2 cut(s) 104, 924
NdeII GATC 2 cut(s) 283, 877
NlaIII CATG 6 cut(s) 61, 108, 145, 485, 928, 1233
NlaIV GGNNCC 2 cut(s) 113, 1003
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 1162
NspI RCATGY 2 cut(s) 61, 485
PaeR7I CTCGAG 1 cut(s) 116
PagI TCATGA 1 cut(s) 1229
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 201
PctI GAATGC 3 cut(s) 178, 191, 1102
PdmI GAANNNNTTC 2 cut(s) 756, 1119
PfeI GAWTC 4 cut(s) 397, 734, 944, 1223
PflFI GACNNNGTC 2 cut(s) 243, 348
PkrI GCNGC 4 cut(s) 102, 280, 576, 1134
PleI GAGTC 2 cut(s) 549, 627
PpsI GAGTC 2 cut(s) 549, 627
PpuMI RGGWCCY 1 cut(s) 112
Psp124BI GAGCTC 1 cut(s) 719
Psp1406I AACGTT 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 112
Psp6I CCWGG 2 cut(s) 569, 588
PspFI CCCAGC 1 cut(s) 1165
PspGI CCWGG 2 cut(s) 569, 588
PspN4I GGNNCC 2 cut(s) 113, 1003
PspPI GGNCC 1 cut(s) 112
PspPPI RGGWCCY 1 cut(s) 112
PspXI VCTCGAGB 1 cut(s) 116
PsyI GACNNNGTC 2 cut(s) 243, 348
RsaI GTAC 2 cut(s) 561, 884
RsaNI GTAC 2 cut(s) 560, 883
RseI CAYNNNNRTG 1 cut(s) 1187
SacI GAGCTC 1 cut(s) 719
SaqAI TTAA 4 cut(s) 9, 80, 155, 1214
SatI GCNGC 4 cut(s) 101, 279, 575, 1133
Sau3AI GATC 2 cut(s) 283, 877
Sau96I GGNCC 1 cut(s) 112
SchI GAGTC 2 cut(s) 550, 628
ScrFI CCNGG 3 cut(s) 571, 590, 941
SduI GDGCHC 2 cut(s) 30, 719
SfaNI GCATC 2 cut(s) 361, 1052
SfcI CTRYAG 2 cut(s) 124, 816
Sfr274I CTCGAG 1 cut(s) 116
SinI GGWCC 1 cut(s) 112
SlaI CTCGAG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 1187
SmlI CTYRAG 4 cut(s) 116, 401, 895, 1177
SmoI CTYRAG 4 cut(s) 116, 401, 895, 1177
Sse9I AATT 3 cut(s) 764, 772, 1096
SsiI CCGC 6 cut(s) 18, 129, 164, 575, 674, 1130
SspMI CTAG 3 cut(s) 342, 867, 1083
SstI GAGCTC 1 cut(s) 719
StyD4I CCNGG 3 cut(s) 569, 588, 939
StyI CCWWGG 3 cut(s) 104, 924, 1082
TaaI ACNGT 3 cut(s) 811, 889, 1155
TaiI ACGT 1 cut(s) 183
TaqI TCGA 7 cut(s) 117, 186, 195, 288, 608, 630, 982
TaqII GACCGA 1 cut(s) 636
TasI AATT 3 cut(s) 764, 772, 1096
TatI WGTACW 1 cut(s) 882
TauI GCSGC 1 cut(s) 577
TfiI GAWTC 4 cut(s) 397, 734, 944, 1223
Tru1I TTAA 4 cut(s) 9, 80, 155, 1214
Tru9I TTAA 4 cut(s) 9, 80, 155, 1214
TscAI CASTG 5 cut(s) 214, 784, 892, 1056, 1194
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 3 cut(s) 100, 278, 1132
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 4 cut(s) 173, 375, 523, 861
TspRI CASTG 5 cut(s) 214, 784, 892, 1056, 1194
Tth111I GACNNNGTC 2 cut(s) 243, 348
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 764
XceI RCATGY 2 cut(s) 61, 485
XcmI CCANNNNNNNNNTGG 2 cut(s) 362, 832
XhoI CTCGAG 1 cut(s) 116
XmaJI CCTAGG 1 cut(s) 1082
XmiI GTMKAC 1 cut(s) 936
XmnI GAANNNNTTC 2 cut(s) 756, 1119
XspI CTAG 3 cut(s) 342, 867, 1083
Zsp2I ATGCAT 1 cut(s) 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.