Rh4AG109400
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003990
Physical Location & Seq
Reverse (-)
23700058 .. 23703404
3347 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG109400.1

Sequence Viewer

Length: 420 bp
ATGGAGCGAATGTATGGCTTCTGTCTCCTCCTCGCCAACCCTATACTAGACAAGACATCTGATACACATGGAAAAATGATAGAGATATTGAGAAGAAGTGATTTTGTTACACAGGAGTTCATATTGAGTCCCTTGCAGTTTGGTGTACCAATAGAAAGCTTTCTTGAATTCAAGAATTGCTGTGATCAGCCGAACTTTGTGGACAACACCACTGTTTATACAGATACCATTGGAGTATTGGAGAAAGATGAAGAGAATGGGTGTTGTACTAGCACCTTGGATCAGTACTCTGTTCCCTCAAGCTTGATAGAGAGATGGGGAAGTGCCATGGATATTGTCTACCCGGATTCAAAGCGATGCTGTTGTTTTACAAAAAGTTATTATCGATATGTGAAGGGTACTGGCTCCCTTTTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

139

Amino Acids

15.82

Weight (kDa)

4.71

Isoelectric Point (pI)

49.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 339
AclWI GGATC 1 cut(s) 288
AcsI RAATTY 1 cut(s) 167
AfaI GTAC 4 cut(s) 147, 268, 287, 400
AgsI TTSAA 3 cut(s) 167, 172, 351
AluBI AGCT 2 cut(s) 159, 303
AluI AGCT 2 cut(s) 159, 303
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 288
ApoI RAATTY 1 cut(s) 167
Asp700I GAANNNNTTC 1 cut(s) 159
AsuC2I CCSGG 1 cut(s) 344
BccI CCATC 1 cut(s) 309
BclI TGATCA 1 cut(s) 184
BcnI CCSGG 1 cut(s) 344
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 2 cut(s) 47, 270
BmcAI AGTACT 1 cut(s) 287
Bme1390I CCNGG 1 cut(s) 344
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 1 cut(s) 344
BmsI GCATC 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 283
BpuMI CCSGG 1 cut(s) 344
Bsa29I ATCGAT 1 cut(s) 385
BsaJI CCNNGG 2 cut(s) 276, 327
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 406
BseCI ATCGAT 1 cut(s) 385
BseDI CCNNGG 2 cut(s) 276, 327
BseNI ACTGG 1 cut(s) 406
BseRI GAGGAG 2 cut(s) 17, 20
BshVI ATCGAT 1 cut(s) 385
BsiSI CCGG 1 cut(s) 344
BslFI GGGAC 1 cut(s) 114
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 114
Bsp143I GATC 2 cut(s) 184, 280
Bsp19I CCATGG 1 cut(s) 327
BspDI ATCGAT 1 cut(s) 385
BspLI GGNNCC 1 cut(s) 406
BspPI GGATC 1 cut(s) 288
BsrI ACTGG 1 cut(s) 406
BssECI CCNNGG 2 cut(s) 276, 327
BssMI GATC 2 cut(s) 184, 280
BssT1I CCWWGG 2 cut(s) 276, 327
Bst4CI ACNGT 1 cut(s) 214
Bst6I CTCTTC 1 cut(s) 246
BstDSI CCRYGG 1 cut(s) 327
BstKTI GATC 2 cut(s) 187, 283
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 2 cut(s) 184, 280
BstSCI CCNGG 1 cut(s) 342
Bsu15I ATCGAT 1 cut(s) 385
BsuTUI ATCGAT 1 cut(s) 385
BtgI CCRYGG 1 cut(s) 327
BtgZI GCGATG 1 cut(s) 370
BtsIMutI CAGTG 1 cut(s) 210
ClaI ATCGAT 1 cut(s) 385
Csp6I GTAC 4 cut(s) 146, 267, 286, 399
CviAII CATG 3 cut(s) 68, 328, 417
CviJI RGCY 5 cut(s) 18, 159, 190, 303, 405
CviKI_1 RGCY 5 cut(s) 18, 159, 190, 303, 405
CviQI GTAC 4 cut(s) 146, 267, 286, 399
DpnI GATC 2 cut(s) 186, 282
DpnII GATC 2 cut(s) 184, 280
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco130I CCWWGG 2 cut(s) 276, 327
EcoRI GAATTC 1 cut(s) 167
EcoT14I CCWWGG 2 cut(s) 276, 327
ErhI CCWWGG 2 cut(s) 276, 327
FaeI CATG 3 cut(s) 71, 331, 420
FaiI YATR 8 cut(s) 15, 44, 69, 122, 219, 329, 390, 418
FaqI GGGAC 1 cut(s) 114
FatI CATG 3 cut(s) 67, 327, 416
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 1 cut(s) 339
FspBI CTAG 2 cut(s) 47, 270
HapII CCGG 1 cut(s) 344
Hin1II CATG 3 cut(s) 71, 331, 420
HindIII AAGCTT 2 cut(s) 157, 301
HinfI GANTC 2 cut(s) 127, 347
HpaII CCGG 1 cut(s) 344
Hpy166II GTNNAC 3 cut(s) 146, 202, 340
Hpy188I TCNGA 1 cut(s) 61
Hpy188III TCNNGA 2 cut(s) 164, 172
Hpy8I GTNNAC 3 cut(s) 146, 202, 340
HpyAV CCTTC 1 cut(s) 388
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4V TGCA 1 cut(s) 136
Hsp92II CATG 3 cut(s) 71, 331, 420
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 2 cut(s) 184, 280
LmnI GCTCC 2 cut(s) 4, 410
LpnPI CCDG 3 cut(s) 98, 357, 387
LweI GCATC 1 cut(s) 347
MaeI CTAG 2 cut(s) 47, 270
MaeIII GTNAC 1 cut(s) 106
MalI GATC 2 cut(s) 186, 282
MboI GATC 2 cut(s) 184, 280
MboII GAAGA 2 cut(s) 105, 263
MluCI AATT 2 cut(s) 167, 175
MlyI GAGTC 1 cut(s) 136
MnlI CCTC 3 cut(s) 38, 41, 307
MroXI GAANNNNTTC 1 cut(s) 159
MspI CCGG 1 cut(s) 344
MspR9I CCNGG 1 cut(s) 344
NciI CCSGG 1 cut(s) 344
NcoI CCATGG 1 cut(s) 327
NdeII GATC 2 cut(s) 184, 280
NlaIII CATG 3 cut(s) 71, 331, 420
NlaIV GGNNCC 1 cut(s) 406
PdmI GAANNNNTTC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 347
PleI GAGTC 1 cut(s) 135
PpsI GAGTC 1 cut(s) 135
PspN4I GGNNCC 1 cut(s) 406
RsaI GTAC 4 cut(s) 147, 268, 287, 400
RsaNI GTAC 4 cut(s) 146, 267, 286, 399
Sau3AI GATC 2 cut(s) 184, 280
ScaI AGTACT 1 cut(s) 287
SchI GAGTC 1 cut(s) 136
ScrFI CCNGG 1 cut(s) 344
SetI ASST 3 cut(s) 161, 278, 305
SfaNI GCATC 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 298
SmoI CTYRAG 1 cut(s) 298
Sse9I AATT 2 cut(s) 167, 175
SspMI CTAG 2 cut(s) 47, 270
StyD4I CCNGG 1 cut(s) 342
StyI CCWWGG 2 cut(s) 276, 327
TaaI ACNGT 1 cut(s) 214
TaqI TCGA 1 cut(s) 385
TasI AATT 2 cut(s) 167, 175
TatI WGTACW 2 cut(s) 266, 285
TfiI GAWTC 1 cut(s) 347
TscAI CASTG 1 cut(s) 217
TspDTI ATGAA 2 cut(s) 109, 264
TspRI CASTG 1 cut(s) 217
XapI RAATTY 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 339
XmnI GAANNNNTTC 1 cut(s) 159
XspI CTAG 2 cut(s) 47, 270
ZrmI AGTACT 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.