Rh4BG194000
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003990
Physical Location & Seq
Reverse (-)
33518743 .. 33521086
2344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG194000.1

Sequence Viewer

Length: 744 bp
ATGACAAGGTTCACAACTAGAGCAGGAGAGAGACCTGGAGGGTCTCTTTGCTTTGGAGAAATGGAGGACACGATGCCCATTTGCAAGACTGATACTCTAACTCCGATCTGGAAGTCCATTGGACTACTGGAAAGCAACTTCGCATTGGACTACTGGAAATCAAAAGGTAACATTCAGATCCTTACTGCAGCTGGTCTCGATAGATATGGAGCGAATGTATGGCTTCTGTCTCCTCCTTGCCAACCCTATACTAGACAAGGTCTCCAAAAGCAATCTGGTGATGCTCAGGCATTTTCATTTCTCCAGATTCTTGAGTTGATACCACACACCTCGCAACCTCCAATAATGTTATTTGTGGAAAATGTTGTTGGATTTGAGACATCTGATACACATGGAAAAATGATAGAGATATTGAGAAGAAGTGATTTTGTTACACAGGAGTTCATATTGAGTCCCTTGCAGTTTGGTGTACCAATAGAAAGCTTTCTTGAATTCAAGAATTGCCGTGATCAGCCGAACTTTGTGGACAACACCACTGTTTATACAGATACCATTGGAGTATTGGAGAAAGATGAAGAGAATGGGTGTTGTACTAGCACCTTGGATCAGTACTCTGTTCCCTCAAGCTTGATAGAGAGATGGGGAAGTGCCATGGATATTGTCTACCCGGATTCAAAGCGATGCTGTTGTTTTACAAAAAGTTATTATCGATATGTGAAGGGTACTGGCTCCCTTTTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

247

Amino Acids

27.79

Weight (kDa)

4.95

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 53 - 158 2.6e-06 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 663
AclWI GGATC 2 cut(s) 172, 612
AcsI RAATTY 1 cut(s) 491
AfaI GTAC 4 cut(s) 471, 592, 611, 724
AgsI TTSAA 3 cut(s) 491, 496, 675
AjnI CCWGG 1 cut(s) 34
AjuI GAANNNNNNNTTGG 2 cut(s) 351, 383
AluBI AGCT 3 cut(s) 191, 483, 627
AluI AGCT 3 cut(s) 191, 483, 627
Alw26I GTCTC 6 cut(s) 25, 48, 200, 234, 266, 371
AlwI GGATC 2 cut(s) 172, 612
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 1 cut(s) 491
Asp700I GAANNNNTTC 1 cut(s) 483
AsuC2I CCSGG 1 cut(s) 668
AsuHPI GGTGA 1 cut(s) 290
BbvI GCAGC 1 cut(s) 200
BccI CCATC 1 cut(s) 633
BceAI ACGGC 1 cut(s) 489
BciT130I CCWGG 1 cut(s) 36
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 1 cut(s) 668
BcoDI GTCTC 6 cut(s) 25, 48, 200, 234, 266, 371
BfaI CTAG 3 cut(s) 18, 252, 594
BfmI CTRYAG 1 cut(s) 186
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
BmcAI AGTACT 1 cut(s) 611
Bme1390I CCNGG 2 cut(s) 36, 668
BmiI GGNNCC 1 cut(s) 730
BmrFI CCNGG 2 cut(s) 36, 668
BmsI GCATC 3 cut(s) 63, 271, 671
BpmI CTGGAG 2 cut(s) 57, 287
Bpu10I CCTNAGC 1 cut(s) 285
BpuEI CTTGAG 2 cut(s) 332, 607
BpuMI CCSGG 1 cut(s) 668
Bsa29I ATCGAT 1 cut(s) 709
BsaI GGTCTC 4 cut(s) 25, 48, 200, 266
BsaJI CCNNGG 2 cut(s) 600, 651
BsaXI ACNNNNNCTCC 6 cut(s) 85, 115, 201, 231, 246, 276
Bse1I ACTGG 3 cut(s) 132, 158, 730
BseBI CCWGG 1 cut(s) 36
BseCI ATCGAT 1 cut(s) 709
BseDI CCNNGG 2 cut(s) 600, 651
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 3 cut(s) 132, 158, 730
BseRI GAGGAG 1 cut(s) 222
BseXI GCAGC 1 cut(s) 200
BshVI ATCGAT 1 cut(s) 709
BsiSI CCGG 1 cut(s) 668
BslFI GGGAC 1 cut(s) 438
BsmAI GTCTC 6 cut(s) 25, 48, 200, 234, 266, 371
BsmFI GGGAC 1 cut(s) 438
Bso31I GGTCTC 4 cut(s) 25, 48, 200, 266
Bsp143I GATC 4 cut(s) 105, 177, 508, 604
Bsp19I CCATGG 1 cut(s) 651
BspCNI CTCAG 1 cut(s) 298
BspDI ATCGAT 1 cut(s) 709
BspLI GGNNCC 1 cut(s) 730
BspMAI CTGCAG 1 cut(s) 190
BspPI GGATC 2 cut(s) 172, 612
BspTNI GGTCTC 4 cut(s) 25, 48, 200, 266
BsrI ACTGG 3 cut(s) 132, 158, 730
BssECI CCNNGG 2 cut(s) 600, 651
BssMI GATC 4 cut(s) 105, 177, 508, 604
BssT1I CCWWGG 2 cut(s) 600, 651
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 1 cut(s) 538
Bst6I CTCTTC 1 cut(s) 570
BstDEI CTNAG 1 cut(s) 285
BstDSI CCRYGG 1 cut(s) 651
BstKTI GATC 4 cut(s) 108, 180, 511, 607
BstMAI GTCTC 6 cut(s) 25, 48, 200, 234, 266, 371
BstMBI GATC 4 cut(s) 105, 177, 508, 604
BstNI CCWGG 1 cut(s) 36
BstSCI CCNGG 2 cut(s) 34, 666
BstSFI CTRYAG 1 cut(s) 186
BstV1I GCAGC 1 cut(s) 200
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
Bsu15I ATCGAT 1 cut(s) 709
BsuTUI ATCGAT 1 cut(s) 709
BtgI CCRYGG 1 cut(s) 651
BtgZI GCGATG 1 cut(s) 694
BtsIMutI CAGTG 1 cut(s) 534
ClaI ATCGAT 1 cut(s) 709
Csp6I GTAC 4 cut(s) 470, 591, 610, 723
CviAII CATG 3 cut(s) 392, 652, 741
CviJI RGCY 6 cut(s) 191, 223, 483, 514, 627, 729
CviKI_1 RGCY 6 cut(s) 191, 223, 483, 514, 627, 729
CviQI GTAC 4 cut(s) 470, 591, 610, 723
DdeI CTNAG 1 cut(s) 285
DpnI GATC 4 cut(s) 107, 179, 510, 606
DpnII GATC 4 cut(s) 105, 177, 508, 604
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Eco130I CCWWGG 2 cut(s) 600, 651
Eco31I GGTCTC 4 cut(s) 25, 48, 200, 266
EcoRI GAATTC 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 34
EcoT14I CCWWGG 2 cut(s) 600, 651
ErhI CCWWGG 2 cut(s) 600, 651
FaeI CATG 3 cut(s) 395, 655, 744
FaiI YATR 9 cut(s) 207, 220, 249, 393, 446, 543, 653, 714, 742
FaqI GGGAC 1 cut(s) 438
FatI CATG 3 cut(s) 391, 651, 740
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 663
Fnu4HI GCNGC 1 cut(s) 189
Fsp4HI GCNGC 1 cut(s) 189
FspBI CTAG 3 cut(s) 18, 252, 594
GluI GCNGC 1 cut(s) 189
GsuI CTGGAG 2 cut(s) 57, 287
HapII CCGG 1 cut(s) 668
Hin1II CATG 3 cut(s) 395, 655, 744
HindIII AAGCTT 2 cut(s) 481, 625
HinfI GANTC 3 cut(s) 307, 451, 671
HpaII CCGG 1 cut(s) 668
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 4 cut(s) 12, 470, 526, 664
Hpy188I TCNGA 3 cut(s) 105, 177, 385
Hpy188III TCNNGA 6 cut(s) 109, 197, 304, 311, 488, 496
Hpy8I GTNNAC 4 cut(s) 12, 470, 526, 664
HpyAV CCTTC 1 cut(s) 712
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4V TGCA 3 cut(s) 84, 188, 460
HpyF3I CTNAG 1 cut(s) 285
Hsp92II CATG 3 cut(s) 395, 655, 744
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 4 cut(s) 105, 177, 508, 604
LmnI GCTCC 2 cut(s) 209, 734
Lsp1109I GCAGC 1 cut(s) 200
LweI GCATC 3 cut(s) 63, 271, 671
MaeI CTAG 3 cut(s) 18, 252, 594
MaeIII GTNAC 2 cut(s) 167, 430
MalI GATC 4 cut(s) 107, 179, 510, 606
MboI GATC 4 cut(s) 105, 177, 508, 604
MboII GAAGA 2 cut(s) 429, 587
MflI RGATCY 1 cut(s) 177
MluCI AATT 2 cut(s) 491, 499
MlyI GAGTC 1 cut(s) 460
MmeI TCCRAC 1 cut(s) 349
MnlI CCTC 6 cut(s) 32, 58, 243, 340, 348, 631
MroXI GAANNNNTTC 1 cut(s) 483
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 1 cut(s) 668
MspR9I CCNGG 2 cut(s) 36, 668
MvaI CCWGG 1 cut(s) 36
NciI CCSGG 1 cut(s) 668
NcoI CCATGG 1 cut(s) 651
NdeII GATC 4 cut(s) 105, 177, 508, 604
NlaIII CATG 3 cut(s) 395, 655, 744
NlaIV GGNNCC 1 cut(s) 730
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 307, 671
PflFI GACNNNGTC 1 cut(s) 258
PkrI GCNGC 1 cut(s) 190
PleI GAGTC 1 cut(s) 459
PpsI GAGTC 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 1 cut(s) 730
PstI CTGCAG 1 cut(s) 190
PsuI RGATCY 1 cut(s) 177
PsyI GACNNNGTC 1 cut(s) 258
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 4 cut(s) 471, 592, 611, 724
RsaNI GTAC 4 cut(s) 470, 591, 610, 723
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 4 cut(s) 105, 177, 508, 604
ScaI AGTACT 1 cut(s) 611
SchI GAGTC 1 cut(s) 460
ScrFI CCNGG 2 cut(s) 36, 668
SfaNI GCATC 3 cut(s) 63, 271, 671
SfcI CTRYAG 1 cut(s) 186
SmlI CTYRAG 2 cut(s) 311, 622
SmoI CTYRAG 2 cut(s) 311, 622
Sse9I AATT 2 cut(s) 491, 499
SspMI CTAG 3 cut(s) 18, 252, 594
StyD4I CCNGG 2 cut(s) 34, 666
StyI CCWWGG 2 cut(s) 600, 651
TaaI ACNGT 1 cut(s) 538
TaqI TCGA 2 cut(s) 198, 709
TasI AATT 2 cut(s) 491, 499
TatI WGTACW 2 cut(s) 590, 609
TfiI GAWTC 2 cut(s) 307, 671
TscAI CASTG 1 cut(s) 541
TseI GCWGC 1 cut(s) 188
TspDTI ATGAA 3 cut(s) 285, 433, 588
TspRI CASTG 1 cut(s) 541
Tth111I GACNNNGTC 1 cut(s) 258
XapI RAATTY 1 cut(s) 491
XcmI CCANNNNNNNNNTGG 3 cut(s) 124, 272, 559
XmiI GTMKAC 1 cut(s) 663
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 3 cut(s) 18, 252, 594
ZrmI AGTACT 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.