Rh2DG337600
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003990
Physical Location & Seq
Forward (+)
43417447 .. 43448967
31521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG337600.1

Sequence Viewer

Length: 882 bp
ATGTTTAAGACGGAAGAAGATCCAGGTTGCTATTCAAGGGCATCTAACTCTGCTTCTGAGCCTGTGGTAGATGTACAAATGAATCTGATCTCAATTGATATGGAGCGAATGTATGGCTTCTGTCTCCTCCTTGCCAACCCTATACTAGACAAGATTCTTGAGTTGATACCACACACCTCGCAACCTCCAATAATGTTATTTGTGGAAAATGTTGTTGGATTTGAGACATCTGATACACATGGAAAAATGATAGAGATATTGAGAAGAAGTGATTTTGTTACACAGGAGTTCATATTGAGTCCCTTGCAGTTTGGTGTACCATATTCCAGGCCACGCTATTTTTGCCCGGCAAAGAGGAAACCGTCAACCTTTCATAGTCAACTTTTCAATAATCAGCTTCTTTGGTCCCCAAAGCCCTTATTTGGGCATACAAATGCGGATGACAAAGTGTTCTGTGAACTTGATGAGCCGCAAGGTTGCTTGGATAAGTTGCTTGAATCTTGTGAGCCAATAGAAAGCTTTCTTGAATTCAAGAATTGCCCTGATCAGCCGAACTTTGTGGACAGCACCACTGTTTATACAGATACCATTAGAGTATTGGAGAAAGATGAAGAGAATGGGTGTTGTACTAGCACCTTGGATCAGTACTCTGTTCCCTCAAGCTTGAGAGAGATGGGGAAGTGCCATGGATATTGTCTACCCGGATTCAAAGCGATGCTGTTGTTTTACAAAAAGTTATTATCGATATGTGAAGGGTACTGGCTCCCTTTTGGCAACTGGGCAGATCAATCAAAGCGGCGTCACCACCATATCGATATCGACGTTGATATCGTTATCCTTTCACACTTCCCCATAAATCCAATTTCTATTTGTTGGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

293

Amino Acids

33.69

Weight (kDa)

5.11

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 697
AciI CCGC 3 cut(s) 437, 470, 796
AclWI GGATC 2 cut(s) 14, 648
AcsI RAATTY 1 cut(s) 527
AcyI GRCGYC 1 cut(s) 799
AfaI GTAC 6 cut(s) 75, 318, 628, 647, 758, 878
AfiI CCNNNNNNNGG 2 cut(s) 422, 423
AgsI TTSAA 6 cut(s) 36, 388, 497, 527, 532, 709
AjnI CCWGG 2 cut(s) 22, 326
AjuI GAANNNNNNNTTGG 2 cut(s) 198, 230
AluBI AGCT 3 cut(s) 397, 519, 663
AluI AGCT 3 cut(s) 397, 519, 663
Alw26I GTCTC 2 cut(s) 128, 218
AlwI GGATC 2 cut(s) 14, 648
AoxI GGCC 1 cut(s) 329
ApoI RAATTY 1 cut(s) 527
Asp700I GAANNNNTTC 1 cut(s) 519
AspS9I GGNCC 1 cut(s) 405
AsuC2I CCSGG 2 cut(s) 347, 702
AsuHPI GGTGA 1 cut(s) 794
AvaII GGWCC 1 cut(s) 405
BccI CCATC 1 cut(s) 667
BciT130I CCWGG 2 cut(s) 24, 328
BclI TGATCA 1 cut(s) 544
BcnI CCSGG 2 cut(s) 347, 702
BcoDI GTCTC 2 cut(s) 128, 218
BfaI CTAG 2 cut(s) 146, 630
BisI GCNGC 2 cut(s) 470, 797
BlsI GCNGC 2 cut(s) 471, 798
BmcAI AGTACT 1 cut(s) 647
Bme1390I CCNGG 4 cut(s) 24, 328, 347, 702
Bme18I GGWCC 1 cut(s) 405
BmgT120I GGNCC 1 cut(s) 405
BmiI GGNNCC 2 cut(s) 407, 764
BmrFI CCNGG 4 cut(s) 24, 328, 347, 702
BmrI ACTGGG 1 cut(s) 787
BmsI GCATC 2 cut(s) 50, 705
BmuI ACTGGG 1 cut(s) 787
BpuEI CTTGAG 3 cut(s) 179, 643, 685
BpuMI CCSGG 2 cut(s) 347, 702
Bsa29I ATCGAT 2 cut(s) 743, 813
BsaHI GRCGYC 1 cut(s) 799
BsaJI CCNNGG 2 cut(s) 636, 685
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 2 cut(s) 422, 423
Bse1I ACTGG 2 cut(s) 764, 782
BseBI CCWGG 2 cut(s) 24, 328
BseCI ATCGAT 2 cut(s) 743, 813
BseDI CCNNGG 2 cut(s) 636, 685
BseGI GGATG 1 cut(s) 445
BseLI CCNNNNNNNGG 2 cut(s) 422, 423
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 2 cut(s) 764, 782
BseRI GAGGAG 1 cut(s) 116
BshFI GGCC 1 cut(s) 331
BshVI ATCGAT 2 cut(s) 743, 813
BsiSI CCGG 2 cut(s) 347, 702
BslFI GGGAC 2 cut(s) 285, 391
BslI CCNNNNNNNGG 2 cut(s) 422, 423
BsmAI GTCTC 2 cut(s) 128, 218
BsmFI GGGAC 2 cut(s) 285, 391
BsnI GGCC 1 cut(s) 331
Bsp1407I TGTACA 1 cut(s) 73
Bsp143I GATC 5 cut(s) 19, 87, 544, 640, 784
Bsp19I CCATGG 1 cut(s) 685
BspACI CCGC 3 cut(s) 437, 470, 796
BspANI GGCC 1 cut(s) 331
BspCNI CTCAG 1 cut(s) 49
BspDI ATCGAT 2 cut(s) 743, 813
BspLI GGNNCC 2 cut(s) 407, 764
BspPI GGATC 2 cut(s) 14, 648
BsrGI TGTACA 1 cut(s) 73
BsrI ACTGG 2 cut(s) 764, 782
BssECI CCNNGG 2 cut(s) 636, 685
BssMI GATC 5 cut(s) 19, 87, 544, 640, 784
BssNI GRCGYC 1 cut(s) 799
BssT1I CCWWGG 2 cut(s) 636, 685
Bst2UI CCWGG 2 cut(s) 24, 328
Bst4CI ACNGT 2 cut(s) 363, 574
Bst6I CTCTTC 1 cut(s) 606
BstACI GRCGYC 1 cut(s) 799
BstAUI TGTACA 1 cut(s) 73
BstDEI CTNAG 1 cut(s) 57
BstDSI CCRYGG 1 cut(s) 685
BstF5I GGATG 1 cut(s) 445
BstKTI GATC 5 cut(s) 22, 90, 547, 643, 787
BstMAI GTCTC 2 cut(s) 128, 218
BstMBI GATC 5 cut(s) 19, 87, 544, 640, 784
BstMWI GCNNNNNNNGC 1 cut(s) 342
BstNI CCWGG 2 cut(s) 24, 328
BstSCI CCNGG 4 cut(s) 22, 326, 345, 700
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
Bsu15I ATCGAT 2 cut(s) 743, 813
BsuRI GGCC 1 cut(s) 331
BsuTUI ATCGAT 2 cut(s) 743, 813
BtgI CCRYGG 1 cut(s) 685
BtgZI GCGATG 1 cut(s) 728
BtsCI GGATG 1 cut(s) 445
BtsIMutI CAGTG 1 cut(s) 570
Cfr13I GGNCC 1 cut(s) 405
ClaI ATCGAT 2 cut(s) 743, 813
CseI GACGC 1 cut(s) 788
Csp6I GTAC 6 cut(s) 74, 317, 627, 646, 757, 877
CviAII CATG 2 cut(s) 239, 686
CviQI GTAC 6 cut(s) 74, 317, 627, 646, 757, 877
DdeI CTNAG 1 cut(s) 57
DpnI GATC 5 cut(s) 21, 89, 546, 642, 786
DpnII GATC 5 cut(s) 19, 87, 544, 640, 784
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Eco130I CCWWGG 2 cut(s) 636, 685
Eco32I GATATC 2 cut(s) 817, 829
Eco47I GGWCC 1 cut(s) 405
EcoRI GAATTC 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 22, 326
EcoRV GATATC 2 cut(s) 817, 829
EcoT14I CCWWGG 2 cut(s) 636, 685
ErhI CCWWGG 2 cut(s) 636, 685
FaeI CATG 2 cut(s) 242, 689
FaqI GGGAC 2 cut(s) 285, 391
FatI CATG 2 cut(s) 238, 685
FbaI TGATCA 1 cut(s) 544
FblI GTMKAC 1 cut(s) 697
Fnu4HI GCNGC 2 cut(s) 470, 797
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 2 cut(s) 470, 797
FspBI CTAG 2 cut(s) 146, 630
GluI GCNGC 2 cut(s) 470, 797
HaeIII GGCC 1 cut(s) 331
HapII CCGG 2 cut(s) 347, 702
HgaI GACGC 1 cut(s) 788
Hin1I GRCGYC 1 cut(s) 799
Hin1II CATG 2 cut(s) 242, 689
HincII GTYRAC 2 cut(s) 366, 380
HindII GTYRAC 2 cut(s) 366, 380
HindIII AAGCTT 2 cut(s) 517, 661
HinfI GANTC 5 cut(s) 82, 154, 298, 497, 705
HpaII CCGG 2 cut(s) 347, 702
HphI GGTGA 1 cut(s) 794
Hpy166II GTNNAC 6 cut(s) 317, 366, 380, 458, 562, 698
Hpy188I TCNGA 3 cut(s) 58, 87, 232
Hpy188III TCNNGA 3 cut(s) 158, 524, 532
Hpy8I GTNNAC 6 cut(s) 317, 366, 380, 458, 562, 698
Hpy99I CGWCG 1 cut(s) 824
HpyAV CCTTC 1 cut(s) 746
HpyCH4III ACNGT 2 cut(s) 363, 574
HpyCH4IV ACGT 1 cut(s) 822
HpyCH4V TGCA 1 cut(s) 307
HpyF10VI GCNNNNNNNGC 1 cut(s) 342
HpyF3I CTNAG 1 cut(s) 57
HpySE526I ACGT 1 cut(s) 822
Hsp92I GRCGYC 1 cut(s) 799
Hsp92II CATG 2 cut(s) 242, 689
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 5 cut(s) 19, 87, 544, 640, 784
LmnI GCTCC 2 cut(s) 103, 768
LweI GCATC 2 cut(s) 50, 705
MaeI CTAG 2 cut(s) 146, 630
MaeII ACGT 1 cut(s) 822
MaeIII GTNAC 2 cut(s) 277, 800
MalI GATC 5 cut(s) 21, 89, 546, 642, 786
MboI GATC 5 cut(s) 19, 87, 544, 640, 784
MboII GAAGA 4 cut(s) 26, 29, 276, 623
MfeI CAATTG 1 cut(s) 93
MflI RGATCY 1 cut(s) 19
MluCI AATT 4 cut(s) 93, 527, 535, 861
MlyI GAGTC 1 cut(s) 307
MmeI TCCRAC 1 cut(s) 196
MnlI CCTC 5 cut(s) 137, 187, 195, 348, 667
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 432
MspI CCGG 2 cut(s) 347, 702
MspR9I CCNGG 4 cut(s) 24, 328, 347, 702
MunI CAATTG 1 cut(s) 93
MvaI CCWGG 2 cut(s) 24, 328
MwoI GCNNNNNNNGC 1 cut(s) 342
NciI CCSGG 2 cut(s) 347, 702
NcoI CCATGG 1 cut(s) 685
NdeII GATC 5 cut(s) 19, 87, 544, 640, 784
NlaIII CATG 2 cut(s) 242, 689
NlaIV GGNNCC 2 cut(s) 407, 764
NmuCI GTSAC 1 cut(s) 800
PcsI WCGNNNNNNNCGW 2 cut(s) 819, 828
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 4 cut(s) 82, 154, 497, 705
PkrI GCNGC 2 cut(s) 471, 798
PleI GAGTC 1 cut(s) 306
PpsI GAGTC 1 cut(s) 306
Psp6I CCWGG 2 cut(s) 22, 326
PspGI CCWGG 2 cut(s) 22, 326
PspN4I GGNNCC 2 cut(s) 407, 764
PspPI GGNCC 1 cut(s) 405
PsuI RGATCY 1 cut(s) 19
RsaI GTAC 6 cut(s) 75, 318, 628, 647, 758, 878
RsaNI GTAC 6 cut(s) 74, 317, 627, 646, 757, 877
RseI CAYNNNNRTG 1 cut(s) 432
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 2 cut(s) 470, 797
Sau3AI GATC 5 cut(s) 19, 87, 544, 640, 784
Sau96I GGNCC 1 cut(s) 405
ScaI AGTACT 1 cut(s) 647
SchI GAGTC 1 cut(s) 307
ScrFI CCNGG 4 cut(s) 24, 328, 347, 702
SfaNI GCATC 2 cut(s) 50, 705
SinI GGWCC 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 3 cut(s) 158, 658, 664
SmoI CTYRAG 3 cut(s) 158, 658, 664
Sse9I AATT 4 cut(s) 93, 527, 535, 861
SsiI CCGC 3 cut(s) 437, 470, 796
SspMI CTAG 2 cut(s) 146, 630
StyD4I CCNGG 4 cut(s) 22, 326, 345, 700
StyI CCWWGG 2 cut(s) 636, 685
TaaI ACNGT 2 cut(s) 363, 574
TaiI ACGT 1 cut(s) 825
TaqI TCGA 3 cut(s) 743, 813, 819
TasI AATT 4 cut(s) 93, 527, 535, 861
TatI WGTACW 3 cut(s) 73, 626, 645
TauI GCSGC 2 cut(s) 472, 799
TfiI GAWTC 4 cut(s) 82, 154, 497, 705
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TscAI CASTG 1 cut(s) 577
TseFI GTSAC 1 cut(s) 800
Tsp45I GTSAC 1 cut(s) 800
TspDTI ATGAA 4 cut(s) 95, 280, 362, 624
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 1 cut(s) 577
VpaK11BI GGWCC 1 cut(s) 405
XapI RAATTY 1 cut(s) 527
XcmI CCANNNNNNNNNTGG 1 cut(s) 595
XmiI GTMKAC 1 cut(s) 697
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 2 cut(s) 146, 630
ZrmI AGTACT 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.