Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002111

Physical Location & Seq

Genomic Coordinates Reverse (-)

9734896 .. 9736250

Gene Structure 1355 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G27570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1353 bp

ATGATGAACACTTCTTCACATTTAAAGGCACAAGCTTCGTGTCCACTTGTAGAACACTTTCTCCGTCGGAAACTTTCCAAGGAAAACTTCGACAGATTCATACCGAATAGATCGGCCATGGATTTCGACTTTGCTAACTACGCTCTTACCCAAGGAAGAAAACGTAATGTCGATGAGGTAACTTCAGCATCAAGAAAAGCCTACATGACGCAATTGGCTGAGGCTATGAATCAGAATCGCACTCGGATTCTCGCTTTCAGAAACAAACCTAAAGCTCTGCTTTCTAGTAATCACTCTGATCCTCCTCACCAACAACCTATATCCGTAAAGCCTCGTAGATACATTCCTCAGAATAGTGAGAGAGTCTTGGATGCACCTGGAATTGCGGATGACTTCTACCTTAACTTGTTGGATTGGGGCAGTTCTAATGTCTTAGCCATTGCTTTGGGAGACACTGTTTACTTGTGGGATGCGTCTAGTGGTTCTACATATAAGCTTGTGACCATTGATGAAGAGGAGGGACCTGTCACAAGTATTAACTGGACGCAAGATGGTCTTGATCTGGCAATTGGTCTTGACAACAGTGAAGTCCAGCTTTGGGATTGTGTTTCCAATCGTCAAGTGAGAACACTGAGAGGTGGTCACGAGTCAAGAGTAGGATCATTGGCATGGAACAATCACATTCTAACAACTGGAGGAATGGATGGAAAGATCGTCAACAATGATGTGAGGATCAGATCATCTATTGTTGAAACTTACTTGGGTCACACAGAAGAGGTTTGTGGTCTTAAGTGGTCAGAATCTGGAAAAAAACTAGCAAGTGGTGGTAACGACAATGTAGTACACATTTGGGATCATCGTTCTGTCGCTTCCTCAAACCCAACTAGGCAATGGCTGCATAGGTTTGAGGAACATACAGCTGCAGTGAGAGCTCTTGCTTGGTGCCCTTTCCAAGCGAGTTTGCTTGCAACTGGTGGTGGTGTAGGTGATGGGAAGATTAAGTTCTGGAATACACACACTGGGGCTTGCTTGAATTCAGTAGAAACTGGCTCTCAAGTTTGTTCATTGTTATGGAGCAAAAGTGAAAGAGAGTTGCTTAGCTCACATGGGTTTACACAGAACCAGCTTACACTTTGGAAGTACCCATCTATGGTGAAAATGGCTGAACTCAATGGTCATACATCAAGAGTTCTTTTTATGGCACAGAGTCCGGATGGTTGTACCGTGGCTTCTGCAGCCGGTGACGAGACTCTCAGGCTTTGGAATGTTTTTGGAGAACCACCTAAGACCACCAAGAAAGCTGCTTCAAAAAAATATACGGATCCATTTGCTCATGTGAATCATATTAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0019899 GO:0019900 GO:0031461 GO:0032991 GO:0033597 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0080008 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 ko04120 ko05166 ko05203 map04110 map04111 map04113 map04114 map04120 map05166 map05203

Pfam Domains

Protein Families

ANAPC4_WD40 WD40

Protein Analysis

450

Amino Acids

50.1

Weight (kDa)

8.57

Isoelectric Point (pI)

40.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	123 - 421	6e-124	CDC20/Fizzy WD40 domain
Beta-prop_WDR19_1st	PF23389	137 - 238	1.8e-06	WDR19 first beta-propeller
Beta-prop_THOC3	PF25174	142 - 291	1.7e-18	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	142 - 286	4.8e-07	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd	PF25168	144 - 288	6.4e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	145 - 288	1.6e-17	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	145 - 244	4e-12	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	146 - 223	4.8e-08	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	150 - 288	1.1e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd	PF25172	193 - 300	1e-07	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	193 - 315	7e-09	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	225 - 422	2.6e-22	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	230 - 380	1.9e-16	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	248 - 356	6.3e-13	WDR5 beta-propeller domain
WD40	PF00400	250 - 285	2.1e-07	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	251 - 347	9.1e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	256 - 366	1.3e-20	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	298 - 423	4.2e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML	PF23409	302 - 436	1.2e-07	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5	PF25175	303 - 423	2.7e-11	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	309 - 423	7e-08	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	310 - 427	3.5e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3	PF25174	352 - 423	2.8e-09	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	353 - 426	1e-06	WDR3 first beta-propeller domain
WD40	PF00400	387 - 422	3.7e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002111)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33260 AT4G33260 AT4G33270 AT5G26900 AT5G27080 AT5G27570
fragaria_vesca	FvH4_6g21090 FvH4_7g33570
malus_domestica	MD01G1233500.v1.1 MD04G1021900.v1.1 MD07G1305600.v1.1
prunus_persica	Prupe.1G099100_v2.0.a1 Prupe.1G099100_v2.0.a1 Prupe.1G099100_v2.0.a1 Prupe.2G129000_v2.0.a1 Prupe.2G129000_v2.0.a1 Prupe.2G323700_v2.0.a1 Prupe.7G086200_v2.0.a1 Prupe.7G089100_v2.0.a1
pyrus_communis	pycom01g24180 pycom04g01810 pycom07g27890
rosa_chinensis	RchiOBHm_Chr1g0383831 RchiOBHm_Chr3g0477471
rosa_laevigata	RLG00000023716 RLG00000026093
rosa_multiflora	Rmu_co8406979.1_g000001 Rmu_sc0033596.1_g000001 Rmu_ssc0000341.1_g000010
rosa_roxburghii	Rroxscaffold_4G00277200 Rroxscaffold_6G00403920 Rroxscaffold_6G00404600
rosa_rugosa	Rorug01G0442500 Rorug03G0161400
rosa_samantha	Rh1AG468300 Rh1BG426000 Rh1CG438700 Rh1DG458800 Rh3AG211400 Rh3BG244900 Rh3CG238800 Rh3DG238200
rosa_wichuraiana	Rw1G040410 Rw3G019160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	942
AccIII	TCCGGA	1 cut(s)	1210
AciI	CCGC	1 cut(s)	386
AclWI	GGATC	6 cut(s)	293, 667, 740, 861, 1316, 1329
AcoI	YGGCCR	1 cut(s)	114
AcsI	RAATTY	1 cut(s)	1033
AcuI	CTGAAG	1 cut(s)	168
AfaI	GTAC	3 cut(s)	843, 1142, 1222
AfiI	CCNNNNNNNGG	2 cut(s)	598, 1150
AflII	CTTAAG	1 cut(s)	788
AgsI	TTSAA	3 cut(s)	752, 1033, 1308
AjnI	CCWGG	1 cut(s)	376
AjuI	GAANNNNNNNTTGG	2 cut(s)	71, 103
AloI	GAACNNNNNNTCC	2 cut(s)	45, 77
AluBI	AGCT	9 cut(s)	35, 275, 496, 595, 920, 932, 1101, 1126, 1301
AluI	AGCT	9 cut(s)	35, 275, 496, 595, 920, 932, 1101, 1126, 1301
Alw21I	GWGCWC	1 cut(s)	934
Alw26I	GTCTC	2 cut(s)	444, 1241
AlwI	GGATC	6 cut(s)	293, 667, 740, 861, 1316, 1329
AlwNI	CAGNNNCTG	1 cut(s)	803
Aor13HI	TCCGGA	1 cut(s)	1210
AoxI	GGCC	1 cut(s)	114
ApeKI	GCWGC	4 cut(s)	895, 920, 1235, 1301
ApoI	RAATTY	1 cut(s)	1033
Asp700I	GAANNNNTTC	1 cut(s)	57
AspS9I	GGNCC	1 cut(s)	521
AsuHPI	GGTGA	4 cut(s)	299, 998, 1165, 1253
AvaII	GGWCC	1 cut(s)	521
BaeGI	GKGCMC	1 cut(s)	947
BamHI	GGATCC	1 cut(s)	1321
BanI	GGYRCC	1 cut(s)	942
BanII	GRGCYC	1 cut(s)	934
BarI	GAAGNNNNNNTAC	2 cut(s)	1213, 1245
BauI	CACGAG	1 cut(s)	644
Bbv12I	GWGCWC	1 cut(s)	934
BbvCI	CCTCAGC	1 cut(s)	219
BbvI	GCAGC	4 cut(s)	882, 907, 1247, 1288
BccI	CCATC	5 cut(s)	545, 698, 983, 1153, 1208
BcgI	CGANNNNNNTGC	2 cut(s)	18, 52
BciT130I	CCWGG	1 cut(s)	378
BcoDI	GTCTC	2 cut(s)	444, 1241
BfaI	CTAG	4 cut(s)	285, 477, 815, 885
BfmI	CTRYAG	2 cut(s)	921, 1233
BfrI	CTTAAG	1 cut(s)	788
BisI	GCNGC	4 cut(s)	896, 921, 1236, 1302
BlpI	GCTNAGC	1 cut(s)	1097
BlsI	GCNGC	4 cut(s)	897, 922, 1237, 1303
Bme1390I	CCNGG	1 cut(s)	378
Bme18I	GGWCC	1 cut(s)	521
BmgT120I	GGNCC	1 cut(s)	521
BmiI	GGNNCC	3 cut(s)	522, 944, 1323
BmrFI	CCNGG	1 cut(s)	378
BmrI	ACTGGG	1 cut(s)	1029
BmsI	GCATC	3 cut(s)	197, 361, 460
BmuI	ACTGGG	1 cut(s)	1029
BpmI	CTGGAG	1 cut(s)	714
Bpu10I	CCTNAGC	1 cut(s)	219
Bpu1102I	GCTNAGC	1 cut(s)	1097
BpuEI	CTTGAG	1 cut(s)	1038
BsaJI	CCNNGG	4 cut(s)	78, 117, 151, 1224
BsaWI	WCCGGW	1 cut(s)	1210
BsaXI	ACNNNNNCTCC	6 cut(s)	45, 75, 441, 471, 1066, 1096
Bsc4I	CCNNNNNNNGG	2 cut(s)	598, 1150
Bse118I	RCCGGY	1 cut(s)	1238
Bse1I	ACTGG	5 cut(s)	545, 697, 976, 1024, 1051
Bse3DI	GCAATG	2 cut(s)	438, 896
BseAI	TCCGGA	1 cut(s)	1210
BseBI	CCWGG	1 cut(s)	378
BseDI	CCNNGG	4 cut(s)	78, 117, 151, 1224
BseGI	GGATG	5 cut(s)	376, 394, 475, 709, 1219
BseLI	CCNNNNNNNGG	2 cut(s)	598, 1150
BseMI	GCAATG	2 cut(s)	438, 896
BseMII	CTCAG	4 cut(s)	210, 362, 623, 1267
BseNI	ACTGG	5 cut(s)	545, 697, 976, 1024, 1051
BseRI	GAGGAG	2 cut(s)	294, 530
BseSI	GKGCMC	1 cut(s)	947
BseXI	GCAGC	4 cut(s)	882, 907, 1247, 1288
BshFI	GGCC	1 cut(s)	116
BshNI	GGYRCC	1 cut(s)	942
BsiHKAI	GWGCWC	1 cut(s)	934
BsiSI	CCGG	2 cut(s)	1211, 1239
BslFI	GGGAC	1 cut(s)	534
BslI	CCNNNNNNNGG	2 cut(s)	598, 1150
BsmAI	GTCTC	2 cut(s)	444, 1241
BsmFI	GGGAC	1 cut(s)	534
BsnI	GGCC	1 cut(s)	116
Bsp1286I	GDGCHC	2 cut(s)	934, 947
Bsp13I	TCCGGA	1 cut(s)	1210
Bsp143I	GATC	9 cut(s)	110, 298, 559, 659, 711, 732, 737, 853, 1321
Bsp1720I	GCTNAGC	1 cut(s)	1097
Bsp19I	CCATGG	1 cut(s)	117
BspACI	CCGC	1 cut(s)	386
BspANI	GGCC	1 cut(s)	116
BspCNI	CTCAG	4 cut(s)	211, 361, 624, 1266
BspEI	TCCGGA	1 cut(s)	1210
BspLI	GGNNCC	3 cut(s)	522, 944, 1323
BspMAI	CTGCAG	2 cut(s)	925, 1237
BspPI	GGATC	6 cut(s)	293, 667, 740, 861, 1316, 1329
BspT107I	GGYRCC	1 cut(s)	942
BspTI	CTTAAG	1 cut(s)	788
BsrDI	GCAATG	2 cut(s)	438, 896
BsrFI	RCCGGY	1 cut(s)	1238
BsrI	ACTGG	5 cut(s)	545, 697, 976, 1024, 1051
BssAI	RCCGGY	1 cut(s)	1238
BssECI	CCNNGG	4 cut(s)	78, 117, 151, 1224
BssMI	GATC	9 cut(s)	110, 298, 559, 659, 711, 732, 737, 853, 1321
BssSI	CACGAG	1 cut(s)	644
BssT1I	CCWWGG	3 cut(s)	78, 117, 151
Bst2BI	CACGAG	1 cut(s)	644
Bst2UI	CCWGG	1 cut(s)	378
Bst4CI	ACNGT	3 cut(s)	457, 584, 1225
Bst6I	CTCTTC	2 cut(s)	507, 768
BstAFI	CTTAAG	1 cut(s)	788
BstAPI	GCANNNNNTGC	1 cut(s)	895
BstC8I	GCNNGC	2 cut(s)	966, 1027
BstDEI	CTNAG	7 cut(s)	219, 348, 433, 632, 1097, 1253, 1284
BstDSI	CCRYGG	2 cut(s)	117, 1224
BstF5I	GGATG	5 cut(s)	376, 394, 475, 709, 1219
BstKTI	GATC	9 cut(s)	113, 301, 562, 662, 714, 735, 740, 856, 1324
BstMAI	GTCTC	2 cut(s)	444, 1241
BstMBI	GATC	9 cut(s)	110, 298, 559, 659, 711, 732, 737, 853, 1321
BstMWI	GCNNNNNNNGC	4 cut(s)	140, 895, 929, 1235
BstNI	CCWGG	1 cut(s)	378
BstSCI	CCNGG	1 cut(s)	376
BstSFI	CTRYAG	2 cut(s)	921, 1233
BstSLI	GKGCMC	1 cut(s)	947
BstV1I	GCAGC	4 cut(s)	882, 907, 1247, 1288
BstX2I	RGATCY	1 cut(s)	1321
BstXI	CCANNNNNNTGG	1 cut(s)	445
BstYI	RGATCY	1 cut(s)	1321
BsuRI	GGCC	1 cut(s)	116
BtgI	CCRYGG	2 cut(s)	117, 1224
BtsCI	GGATG	5 cut(s)	376, 394, 475, 709, 1219
BtsI	GCAGTG	1 cut(s)	930
BtsIMutI	CAGTG	5 cut(s)	453, 589, 629, 930, 1017
Cac8I	GCNNGC	2 cut(s)	966, 1027
CaiI	CAGNNNCTG	1 cut(s)	803
Cfr10I	RCCGGY	1 cut(s)	1238
Cfr13I	GGNCC	1 cut(s)	521
CseI	GACGC	3 cut(s)	217, 462, 553
Csp6I	GTAC	3 cut(s)	842, 1141, 1221
CviAII	CATG	5 cut(s)	118, 205, 669, 1106, 1334
CviQI	GTAC	3 cut(s)	842, 1141, 1221
DdeI	CTNAG	7 cut(s)	219, 348, 433, 632, 1097, 1253, 1284
DpnI	GATC	9 cut(s)	112, 300, 561, 661, 713, 734, 739, 855, 1323
DpnII	GATC	9 cut(s)	110, 298, 559, 659, 711, 732, 737, 853, 1321
DraI	TTTAAA	1 cut(s)	24
EaeI	YGGCCR	1 cut(s)	114
Eam1104I	CTCTTC	2 cut(s)	507, 768
EarI	CTCTTC	2 cut(s)	507, 768
Ecl136II	GAGCTC	1 cut(s)	932
Eco130I	CCWWGG	3 cut(s)	78, 117, 151
Eco24I	GRGCYC	1 cut(s)	934
Eco47I	GGWCC	1 cut(s)	521
Eco53kI	GAGCTC	1 cut(s)	932
Eco57I	CTGAAG	1 cut(s)	168
EcoICRI	GAGCTC	1 cut(s)	932
EcoO109I	RGGNCCY	1 cut(s)	521
EcoRI	GAATTC	1 cut(s)	1033
EcoRII	CCWGG	1 cut(s)	376
EcoT14I	CCWWGG	3 cut(s)	78, 117, 151
EcoT38I	GRGCYC	1 cut(s)	934
ErhI	CCWWGG	3 cut(s)	78, 117, 151
FaeI	CATG	5 cut(s)	121, 208, 672, 1109, 1337
FalI	AAGNNNNNCTT	8 cut(s)	71, 103, 264, 296, 540, 572, 579, 611
FaqI	GGGAC	1 cut(s)	534
FatI	CATG	5 cut(s)	117, 204, 668, 1105, 1333
Fnu4HI	GCNGC	4 cut(s)	896, 921, 1236, 1302
FokI	GGATG	5 cut(s)	383, 401, 482, 716, 1226
FriOI	GRGCYC	1 cut(s)	934
Fsp4HI	GCNGC	4 cut(s)	896, 921, 1236, 1302
FspBI	CTAG	4 cut(s)	285, 477, 815, 885
GluI	GCNGC	4 cut(s)	896, 921, 1236, 1302
GsuI	CTGGAG	1 cut(s)	714
HaeIII	GGCC	1 cut(s)	116
HapII	CCGG	2 cut(s)	1211, 1239
HgaI	GACGC	3 cut(s)	217, 462, 553
Hin1II	CATG	5 cut(s)	121, 208, 672, 1109, 1337
HincII	GTYRAC	1 cut(s)	718
HindII	GTYRAC	1 cut(s)	718
HindIII	AAGCTT	2 cut(s)	33, 494
HpaII	CCGG	2 cut(s)	1211, 1239
HphI	GGTGA	4 cut(s)	299, 998, 1165, 1253
Hpy166II	GTNNAC	5 cut(s)	44, 460, 718, 844, 1113
Hpy188I	TCNGA	8 cut(s)	69, 234, 246, 260, 298, 351, 737, 799
Hpy188III	TCNNGA	9 cut(s)	192, 557, 575, 644, 651, 804, 1006, 1185, 1211
Hpy8I	GTNNAC	5 cut(s)	44, 460, 718, 844, 1113
Hpy99I	CGWCG	1 cut(s)	69
HpyCH4III	ACNGT	3 cut(s)	457, 584, 1225
HpyCH4IV	ACGT	1 cut(s)	163
HpyCH4V	TGCA	5 cut(s)	374, 898, 923, 968, 1235
HpyF10VI	GCNNNNNNNGC	4 cut(s)	140, 895, 929, 1235
HpyF3I	CTNAG	7 cut(s)	219, 348, 433, 632, 1097, 1253, 1284
HpySE526I	ACGT	1 cut(s)	163
Hsp92II	CATG	5 cut(s)	121, 208, 672, 1109, 1337
Kpn2I	TCCGGA	1 cut(s)	1210
Kzo9I	GATC	9 cut(s)	110, 298, 559, 659, 711, 732, 737, 853, 1321
LmnI	GCTCC	1 cut(s)	1074
Lsp1109I	GCAGC	4 cut(s)	882, 907, 1247, 1288
LweI	GCATC	3 cut(s)	197, 361, 460
MaeI	CTAG	4 cut(s)	285, 477, 815, 885
MaeII	ACGT	1 cut(s)	163
MaeIII	GTNAC	7 cut(s)	178, 499, 526, 641, 764, 827, 1241
MalI	GATC	9 cut(s)	112, 300, 561, 661, 713, 734, 739, 855, 1323
MboI	GATC	9 cut(s)	110, 298, 559, 659, 711, 732, 737, 853, 1321
MboII	GAAGA	5 cut(s)	6, 168, 524, 785, 1006
MfeI	CAATTG	2 cut(s)	212, 567
MflI	RGATCY	1 cut(s)	1321
MhlI	GDGCHC	2 cut(s)	934, 947
MluCI	AATT	4 cut(s)	212, 381, 567, 1033
MlyI	GAGTC	4 cut(s)	372, 656, 1216, 1243
MmeI	TCCRAC	2 cut(s)	47, 390
MroI	TCCGGA	1 cut(s)	1210
MroXI	GAANNNNTTC	1 cut(s)	57
MseI	TTAA	5 cut(s)	23, 402, 537, 789, 999
MslI	CAYNNNNRTG	2 cut(s)	667, 1069
MspA1I	CMGCKG	1 cut(s)	920
MspCI	CTTAAG	1 cut(s)	788
MspI	CCGG	2 cut(s)	1211, 1239
MspR9I	CCNGG	1 cut(s)	378
MunI	CAATTG	2 cut(s)	212, 567
MvaI	CCWGG	1 cut(s)	378
MwoI	GCNNNNNNNGC	4 cut(s)	140, 895, 929, 1235
NcoI	CCATGG	1 cut(s)	117
NdeII	GATC	9 cut(s)	110, 298, 559, 659, 711, 732, 737, 853, 1321
NlaIII	CATG	5 cut(s)	121, 208, 672, 1109, 1337
NlaIV	GGNNCC	3 cut(s)	522, 944, 1323
NmuCI	GTSAC	5 cut(s)	499, 526, 641, 764, 1241
PdmI	GAANNNNTTC	1 cut(s)	57
PfeI	GAWTC	6 cut(s)	96, 229, 235, 247, 800, 1339
PkrI	GCNGC	4 cut(s)	897, 922, 1237, 1303
PleI	GAGTC	4 cut(s)	371, 655, 1215, 1243
PpsI	GAGTC	4 cut(s)	371, 655, 1215, 1243
PpuMI	RGGWCCY	1 cut(s)	521
Psp124BI	GAGCTC	1 cut(s)	934
Psp5II	RGGWCCY	1 cut(s)	521
Psp6I	CCWGG	1 cut(s)	376
PspGI	CCWGG	1 cut(s)	376
PspN4I	GGNNCC	3 cut(s)	522, 944, 1323
PspPI	GGNCC	1 cut(s)	521
PspPPI	RGGWCCY	1 cut(s)	521
PstI	CTGCAG	2 cut(s)	925, 1237
PstNI	CAGNNNCTG	1 cut(s)	803
PsuI	RGATCY	1 cut(s)	1321
PvuII	CAGCTG	1 cut(s)	920
RsaI	GTAC	3 cut(s)	843, 1142, 1222
RsaNI	GTAC	3 cut(s)	842, 1141, 1221
RseI	CAYNNNNRTG	2 cut(s)	667, 1069
SacI	GAGCTC	1 cut(s)	934
SaqAI	TTAA	5 cut(s)	23, 402, 537, 789, 999
SatI	GCNGC	4 cut(s)	896, 921, 1236, 1302
Sau3AI	GATC	9 cut(s)	110, 298, 559, 659, 711, 732, 737, 853, 1321
Sau96I	GGNCC	1 cut(s)	521
SchI	GAGTC	4 cut(s)	372, 656, 1216, 1243
ScrFI	CCNGG	1 cut(s)	378
SduI	GDGCHC	2 cut(s)	934, 947
SfaNI	GCATC	3 cut(s)	197, 361, 460
SfcI	CTRYAG	2 cut(s)	921, 1233
SinI	GGWCC	1 cut(s)	521
SmiMI	CAYNNNNRTG	2 cut(s)	667, 1069
SmlI	CTYRAG	2 cut(s)	788, 1053
SmoI	CTYRAG	2 cut(s)	788, 1053
Sse9I	AATT	4 cut(s)	212, 381, 567, 1033
SsiI	CCGC	1 cut(s)	386
SspMI	CTAG	4 cut(s)	285, 477, 815, 885
SstI	GAGCTC	1 cut(s)	934
StyD4I	CCNGG	1 cut(s)	376
StyI	CCWWGG	3 cut(s)	78, 117, 151
TaaI	ACNGT	3 cut(s)	457, 584, 1225
TaiI	ACGT	1 cut(s)	166
TaqI	TCGA	3 cut(s)	90, 126, 171
TasI	AATT	4 cut(s)	212, 381, 567, 1033
TatI	WGTACW	1 cut(s)	841
TfiI	GAWTC	6 cut(s)	96, 229, 235, 247, 800, 1339
Tru1I	TTAA	5 cut(s)	23, 402, 537, 789, 999
Tru9I	TTAA	5 cut(s)	23, 402, 537, 789, 999
TscAI	CASTG	5 cut(s)	460, 589, 636, 930, 1024
TseFI	GTSAC	5 cut(s)	499, 526, 641, 764, 1241
TseI	GCWGC	4 cut(s)	895, 920, 1235, 1301
Tsp45I	GTSAC	5 cut(s)	499, 526, 641, 764, 1241
TspDTI	ATGAA	5 cut(s)	20, 88, 242, 525, 1053
TspGWI	ACGGA	3 cut(s)	53, 313, 1334
TspRI	CASTG	5 cut(s)	460, 589, 636, 930, 1024
Vha464I	CTTAAG	1 cut(s)	788
VpaK11BI	GGWCC	1 cut(s)	521
XapI	RAATTY	1 cut(s)	1033
XcmI	CCANNNNNNNNNTGG	1 cut(s)	888
XmnI	GAANNNNTTC	1 cut(s)	57
XspI	CTAG	4 cut(s)	285, 477, 815, 885

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.