FvH4_6g21090

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002111
Physical Location & Seq
Forward (+)
14673996 .. 14677427
3432 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21090.t1

Sequence Viewer

Length: 1311 bp
ATGGATTCATCAAGAAAGGCCATGTCCCAGTACCCAAGACATCGTAGTCAAGAAAATTTGGATAGGTTCATCCCCAATCGGGAGGCTATAGACATTGGTTATGCCAATGCTGTGCTGACAGAAAGGAGAACAGGCAAAGTGAACCCAGTTGAGAGCTCTGCATCGACTGTGGCGTACCAGAAGCTATTGGCTGAGACGCTCAACATGAATCGGAGAATTCTTACATTCAAGAGCAAGCCTCCTGCGCCTATTGAACTGATTCCGAGGGCAATGTGTTCACCCCCAAATAATAAGGCCAAATCCAGGACCAGGAGACACATTTCTCAGACAGCAGAGAAGATATTGGATGCTCCCGATCTCTCTGATGATTTCTATTTGAAGTTACTGGACTGGGGCACAAACAACATCCTTGCAATAGCTCTTGGAAGCACGGTTTACCTATGGGATGCTTCAAACAGCTCCACCTCAGAGCTTGTTACAGTTTCTGATGATGATGGTCCTGTTACAAGCCTTAGCTGGGCTGCTGATGGAAGGCACATTGCTCTTGGATTAAACAATTCTTGCGTTGAATTGTGGGATTCTATCTCTGTTAAACTGTTACGAAGATTGAGGGGTCACCATGAACGAGTAGGTTCATTGGCATGGAACAAAGAGATTCTCACAACAGGTTCAAGGGATGGAAATATCATCAACAATGATGTAAGAGTTAGATCCCATATGGTTCAAAACTTCTTGGGCCACCAGCAGGAGGTTTGTGGACTAAAATGGTCTGCCTCTGGCTGTCAGCTAGCAAGTGGAGGTAACGATAGTCTGGTTTTCATATGGGATAGGTCATTTGCCTCTCGAAATGCACAGTGGCTTCACAGGTTACAAGATCACACTTCAGCAGTCAAAGCCCTTGCTTGGTGTCCATTCCAAGCGAATTTACTAGCCTCTGGTGGTGGTGTAGGGGATGATACAATTAAGTTCTGGAATGCACACACGGGCTCCTGCTTAAATTCAGTCAACACAGGCTCGCAAGTGTGTGCTCTACTGTGGAACAGCCATGAACGAGAGTTGTTAAGCTCTCAGGGCTTTACAGAGAACCAACTCATCCTCTGGAAGTACCCGTCCATGGTGAGGTTGACAGAGCTCAGTGGTCACACTTCTAGAGTGCTCTATATGACGCAGAGCCCAGATGGGTGCACCGTTGCATCTGCAGCGGACGAAACACTGGGGTTCTGGAATGTGTTTGGGATCCCAGAAGTTGCCAAGCCTGCTCCAAAACAAAGTCTGATGCCATTTTCTCCCAGGTACAACTGCATTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko05166 ko05203 map04110 map04111 map04113 map04114 map04120 map05166 map05203
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

437

Amino Acids

48.4

Weight (kDa)

8.68

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 115 - 408 6.4e-119 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 118 - 218 2.4e-06 WDHD1 first WD40 domain
Beta-prop_WDR19_1st PF23389 128 - 229 5.9e-07 WDR19 first beta-propeller
WD40_Gbeta PF25391 133 - 325 1.5e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 137 - 238 9.7e-13 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 139 - 276 5.1e-18 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 141 - 277 3.4e-17 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 141 - 254 8e-09 TEP-1 second beta-propeller
Beta-prop_EML_2 PF23414 151 - 226 7.8e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 217 - 409 1.7e-17 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 239 - 368 8.3e-17 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 239 - 351 1.4e-14 WDR5 beta-propeller domain
WD40 PF00400 241 - 276 2e-06 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 242 - 336 1.1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 247 - 353 1.6e-19 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 309 - 411 4e-06 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 45
AciI CCGC 1 cut(s) 1202
AclWI GGATC 3 cut(s) 705, 1231, 1244
AcsI RAATTY 4 cut(s) 55, 216, 922, 997
AcuI CTGAAG 1 cut(s) 867
AfaI GTAC 4 cut(s) 32, 176, 1106, 1295
AfiI CCNNNNNNNGG 9 cut(s) 79, 303, 309, 517, 745, 748, 903, 1114, 1119
AgsI TTSAA 7 cut(s) 229, 254, 379, 453, 569, 672, 725
AjnI CCWGG 3 cut(s) 302, 308, 1289
AluBI AGCT 9 cut(s) 156, 184, 419, 459, 472, 516, 787, 1065, 1132
AluI AGCT 9 cut(s) 156, 184, 419, 459, 472, 516, 787, 1065, 1132
Alw21I GWGCWC 5 cut(s) 158, 1030, 1134, 1158, 1187
Alw26I GTCTC 2 cut(s) 188, 307
Alw44I GTGCAC 1 cut(s) 1183
AlwI GGATC 3 cut(s) 705, 1231, 1244
AlwNI CAGNNNCTG 1 cut(s) 485
AoxI GGCC 3 cut(s) 18, 294, 736
ApaLI GTGCAC 1 cut(s) 1183
ApeKI GCWGC 2 cut(s) 521, 1199
ApoI RAATTY 4 cut(s) 55, 216, 922, 997
Asp700I GAANNNNTTC 1 cut(s) 258
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 3 cut(s) 306, 497, 736
AsuHPI GGTGA 3 cut(s) 270, 608, 1129
AsuNHI GCTAGC 1 cut(s) 787
AvaII GGWCC 2 cut(s) 306, 497
BaeGI GKGCMC 2 cut(s) 398, 1187
BamHI GGATCC 1 cut(s) 1236
BanII GRGCYC 4 cut(s) 158, 989, 1134, 1175
Bbv12I GWGCWC 5 cut(s) 158, 1030, 1134, 1158, 1187
BbvI GCAGC 2 cut(s) 508, 1211
BccI CCATC 4 cut(s) 488, 521, 671, 1172
BciT130I CCWGG 3 cut(s) 304, 310, 1291
BcoDI GTCTC 2 cut(s) 188, 307
BfaI CTAG 3 cut(s) 788, 929, 1149
BfmI CTRYAG 2 cut(s) 87, 1197
BisI GCNGC 2 cut(s) 522, 1200
BlsI GCNGC 2 cut(s) 523, 1201
Bme1390I CCNGG 3 cut(s) 304, 310, 1291
Bme18I GGWCC 2 cut(s) 306, 497
BmgT120I GGNCC 3 cut(s) 306, 497, 736
BmiI GGNNCC 2 cut(s) 988, 1238
BmrFI CCNGG 3 cut(s) 304, 310, 1291
BmrI ACTGGG 4 cut(s) 22, 140, 400, 1223
BmsI GCATC 5 cut(s) 170, 337, 436, 1202, 1266
BmtI GCTAGC 1 cut(s) 791
BmuI ACTGGG 4 cut(s) 22, 140, 400, 1223
BplI GAGNNNNNCTC 2 cut(s) 223, 255
Bpu10I CCTNAGC 1 cut(s) 512
BsaJI CCNNGG 3 cut(s) 263, 1113, 1289
BsaXI ACNNNNNCTCC 2 cut(s) 971, 1001
Bsc4I CCNNNNNNNGG 9 cut(s) 79, 303, 309, 517, 745, 748, 903, 1114, 1119
Bse1I ACTGG 5 cut(s) 28, 146, 390, 395, 1218
Bse3DI GCAATG 2 cut(s) 276, 537
BseBI CCWGG 3 cut(s) 304, 310, 1291
BseDI CCNNGG 3 cut(s) 263, 1113, 1289
BseGI GGATG 7 cut(s) 69, 352, 405, 451, 682, 958, 1092
BseLI CCNNNNNNNGG 9 cut(s) 79, 303, 309, 517, 745, 748, 903, 1114, 1119
BseMI GCAATG 2 cut(s) 276, 537
BseMII CTCAG 5 cut(s) 183, 338, 480, 1082, 1147
BseNI ACTGG 5 cut(s) 28, 146, 390, 395, 1218
BseSI GKGCMC 2 cut(s) 398, 1187
BseXI GCAGC 2 cut(s) 508, 1211
BseYI CCCAGC 1 cut(s) 516
BshFI GGCC 3 cut(s) 20, 296, 738
BsiHKAI GWGCWC 5 cut(s) 158, 1030, 1134, 1158, 1187
BslFI GGGAC 1 cut(s) 10
BslI CCNNNNNNNGG 9 cut(s) 79, 303, 309, 517, 745, 748, 903, 1114, 1119
BsmAI GTCTC 2 cut(s) 188, 307
BsmBI CGTCTC 1 cut(s) 188
BsmFI GGGAC 1 cut(s) 10
BsmI GAATGC 2 cut(s) 979, 1302
BsnI GGCC 3 cut(s) 20, 296, 738
Bsp1286I GDGCHC 8 cut(s) 158, 398, 989, 1030, 1134, 1158, 1175, 1187
Bsp143I GATC 4 cut(s) 355, 710, 874, 1236
Bsp19I CCATGG 1 cut(s) 1113
BspACI CCGC 1 cut(s) 1202
BspANI GGCC 3 cut(s) 20, 296, 738
BspCNI CTCAG 5 cut(s) 184, 337, 479, 1081, 1146
BspLI GGNNCC 2 cut(s) 988, 1238
BspMAI CTGCAG 1 cut(s) 1201
BspOI GCTAGC 1 cut(s) 791
BspPI GGATC 3 cut(s) 705, 1231, 1244
BsrDI GCAATG 2 cut(s) 276, 537
BsrI ACTGG 5 cut(s) 28, 146, 390, 395, 1218
BssECI CCNNGG 3 cut(s) 263, 1113, 1289
BssMI GATC 4 cut(s) 355, 710, 874, 1236
BssT1I CCWWGG 1 cut(s) 1113
Bst2UI CCWGG 3 cut(s) 304, 310, 1291
Bst4CI ACNGT 7 cut(s) 169, 433, 481, 597, 855, 1035, 1189
BstC8I GCNNGC 4 cut(s) 236, 789, 1016, 1257
BstDEI CTNAG 6 cut(s) 192, 324, 466, 512, 1068, 1133
BstDSI CCRYGG 1 cut(s) 1113
BstEII GGTNACC 1 cut(s) 614
BstF5I GGATG 7 cut(s) 69, 352, 405, 451, 682, 958, 1092
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 4 cut(s) 358, 713, 877, 1239
BstMAI GTCTC 2 cut(s) 188, 307
BstMBI GATC 4 cut(s) 355, 710, 874, 1236
BstMWI GCNNNNNNNGC 5 cut(s) 244, 893, 1071, 1199, 1256
BstNI CCWGG 3 cut(s) 304, 310, 1291
BstPI GGTNACC 1 cut(s) 614
BstSCI CCNGG 3 cut(s) 302, 308, 1289
BstSFI CTRYAG 2 cut(s) 87, 1197
BstSLI GKGCMC 2 cut(s) 398, 1187
BstV1I GCAGC 2 cut(s) 508, 1211
BstX2I RGATCY 2 cut(s) 710, 1236
BstYI RGATCY 2 cut(s) 710, 1236
BsuRI GGCC 3 cut(s) 20, 296, 738
BtgI CCRYGG 1 cut(s) 1113
BtsCI GGATG 7 cut(s) 69, 352, 405, 451, 682, 958, 1092
BtsIMutI CAGTG 3 cut(s) 860, 1141, 1211
Cac8I GCNNGC 4 cut(s) 236, 789, 1016, 1257
CaiI CAGNNNCTG 1 cut(s) 485
CfoI GCGC 1 cut(s) 247
Cfr13I GGNCC 3 cut(s) 306, 497, 736
CseI GACGC 2 cut(s) 205, 1174
Csp6I GTAC 4 cut(s) 31, 175, 1105, 1294
CviAII CATG 6 cut(s) 22, 205, 620, 642, 1046, 1114
CviQI GTAC 4 cut(s) 31, 175, 1105, 1294
DdeI CTNAG 6 cut(s) 192, 324, 466, 512, 1068, 1133
DpnI GATC 4 cut(s) 357, 712, 876, 1238
DpnII GATC 4 cut(s) 355, 710, 874, 1236
DrdI GACNNNNNNGTC 1 cut(s) 45
DseDI GACNNNNNNGTC 1 cut(s) 45
Ecl136II GAGCTC 2 cut(s) 156, 1132
Eco130I CCWWGG 1 cut(s) 1113
Eco24I GRGCYC 4 cut(s) 158, 989, 1134, 1175
Eco47I GGWCC 2 cut(s) 306, 497
Eco53kI GAGCTC 2 cut(s) 156, 1132
Eco57I CTGAAG 1 cut(s) 867
Eco91I GGTNACC 1 cut(s) 614
EcoICRI GAGCTC 2 cut(s) 156, 1132
EcoO65I GGTNACC 1 cut(s) 614
EcoRI GAATTC 1 cut(s) 216
EcoRII CCWGG 3 cut(s) 302, 308, 1289
EcoT14I CCWWGG 1 cut(s) 1113
EcoT38I GRGCYC 4 cut(s) 158, 989, 1134, 1175
ErhI CCWWGG 1 cut(s) 1113
Esp3I CGTCTC 1 cut(s) 188
FaeI CATG 6 cut(s) 25, 208, 623, 645, 1049, 1117
FaqI GGGAC 1 cut(s) 10
FatI CATG 6 cut(s) 21, 204, 619, 641, 1045, 1113
FauNDI CATATG 2 cut(s) 717, 821
Fnu4HI GCNGC 2 cut(s) 522, 1200
FokI GGATG 7 cut(s) 56, 359, 392, 458, 689, 965, 1079
FriOI GRGCYC 4 cut(s) 158, 989, 1134, 1175
Fsp4HI GCNGC 2 cut(s) 522, 1200
FspBI CTAG 3 cut(s) 788, 929, 1149
GlaI GCGC 1 cut(s) 246
GluI GCNGC 2 cut(s) 522, 1200
GsaI CCCAGC 1 cut(s) 520
HaeIII GGCC 3 cut(s) 20, 296, 738
HgaI GACGC 2 cut(s) 205, 1174
HhaI GCGC 1 cut(s) 247
Hin1II CATG 6 cut(s) 25, 208, 623, 645, 1049, 1117
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HincII GTYRAC 2 cut(s) 1006, 1125
HindII GTYRAC 2 cut(s) 1006, 1125
HinfI GANTC 5 cut(s) 5, 208, 259, 578, 655
HphI GGTGA 3 cut(s) 270, 608, 1129
Hpy166II GTNNAC 7 cut(s) 142, 278, 436, 758, 1006, 1125, 1185
Hpy188I TCNGA 7 cut(s) 213, 264, 327, 364, 469, 487, 1275
Hpy8I GTNNAC 7 cut(s) 142, 278, 436, 758, 1006, 1125, 1185
HpyAV CCTTC 1 cut(s) 525
HpyCH4III ACNGT 7 cut(s) 169, 433, 481, 597, 855, 1035, 1189
HpyCH4V TGCA 8 cut(s) 161, 413, 851, 977, 1185, 1193, 1199, 1302
HpyF10VI GCNNNNNNNGC 5 cut(s) 244, 893, 1071, 1199, 1256
HpyF3I CTNAG 6 cut(s) 192, 324, 466, 512, 1068, 1133
Hsp92II CATG 6 cut(s) 25, 208, 623, 645, 1049, 1117
HspAI GCGC 1 cut(s) 245
Kzo9I GATC 4 cut(s) 355, 710, 874, 1236
LmnI GCTCC 4 cut(s) 355, 464, 992, 1264
Lsp1109I GCAGC 2 cut(s) 508, 1211
LweI GCATC 5 cut(s) 170, 337, 436, 1202, 1266
MaeI CTAG 3 cut(s) 788, 929, 1149
MaeIII GTNAC 8 cut(s) 381, 475, 502, 597, 614, 800, 867, 1139
MalI GATC 4 cut(s) 357, 712, 876, 1238
MboI GATC 4 cut(s) 355, 710, 874, 1236
MboII GAAGA 2 cut(s) 349, 615
MflI RGATCY 2 cut(s) 710, 1236
MhlI GDGCHC 8 cut(s) 158, 398, 989, 1030, 1134, 1158, 1175, 1187
MluCI AATT 7 cut(s) 55, 216, 556, 569, 922, 960, 997
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 5 cut(s) 551, 591, 963, 995, 1061
MslI CAYNNNNRTG 1 cut(s) 640
MspA1I CMGCKG 1 cut(s) 1202
MspR9I CCNGG 3 cut(s) 304, 310, 1291
Mva1269I GAATGC 2 cut(s) 979, 1302
MvaI CCWGG 3 cut(s) 304, 310, 1291
MwoI GCNNNNNNNGC 5 cut(s) 244, 893, 1071, 1199, 1256
NcoI CCATGG 1 cut(s) 1113
NdeI CATATG 2 cut(s) 717, 821
NdeII GATC 4 cut(s) 355, 710, 874, 1236
NheI GCTAGC 1 cut(s) 787
NlaIII CATG 6 cut(s) 25, 208, 623, 645, 1049, 1117
NlaIV GGNNCC 2 cut(s) 988, 1238
NmuCI GTSAC 2 cut(s) 614, 1139
PcsI WCGNNNNNNNCGW 1 cut(s) 170
PctI GAATGC 2 cut(s) 979, 1302
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 5 cut(s) 5, 208, 259, 578, 655
PfoI TCCNGGA 1 cut(s) 302
PkrI GCNGC 2 cut(s) 523, 1201
Psp124BI GAGCTC 2 cut(s) 158, 1134
Psp6I CCWGG 3 cut(s) 302, 308, 1289
PspEI GGTNACC 1 cut(s) 614
PspFI CCCAGC 1 cut(s) 516
PspGI CCWGG 3 cut(s) 302, 308, 1289
PspN4I GGNNCC 2 cut(s) 988, 1238
PspPI GGNCC 3 cut(s) 306, 497, 736
PstI CTGCAG 1 cut(s) 1201
PstNI CAGNNNCTG 1 cut(s) 485
PsuI RGATCY 2 cut(s) 710, 1236
RsaI GTAC 4 cut(s) 32, 176, 1106, 1295
RsaNI GTAC 4 cut(s) 31, 175, 1105, 1294
RseI CAYNNNNRTG 1 cut(s) 640
SacI GAGCTC 2 cut(s) 158, 1134
SaqAI TTAA 5 cut(s) 551, 591, 963, 995, 1061
SatI GCNGC 2 cut(s) 522, 1200
Sau3AI GATC 4 cut(s) 355, 710, 874, 1236
Sau96I GGNCC 3 cut(s) 306, 497, 736
ScrFI CCNGG 3 cut(s) 304, 310, 1291
SduI GDGCHC 8 cut(s) 158, 398, 989, 1030, 1134, 1158, 1175, 1187
SfaNI GCATC 5 cut(s) 170, 337, 436, 1202, 1266
SfcI CTRYAG 2 cut(s) 87, 1197
SinI GGWCC 2 cut(s) 306, 497
SmiMI CAYNNNNRTG 1 cut(s) 640
Sse9I AATT 7 cut(s) 55, 216, 556, 569, 922, 960, 997
SsiI CCGC 1 cut(s) 1202
SspMI CTAG 3 cut(s) 788, 929, 1149
SstI GAGCTC 2 cut(s) 158, 1134
StyD4I CCNGG 3 cut(s) 302, 308, 1289
StyI CCWWGG 1 cut(s) 1113
TaaI ACNGT 7 cut(s) 169, 433, 481, 597, 855, 1035, 1189
TaqI TCGA 2 cut(s) 164, 844
TasI AATT 7 cut(s) 55, 216, 556, 569, 922, 960, 997
TfiI GAWTC 5 cut(s) 5, 208, 259, 578, 655
Tru1I TTAA 5 cut(s) 551, 591, 963, 995, 1061
Tru9I TTAA 5 cut(s) 551, 591, 963, 995, 1061
TscAI CASTG 3 cut(s) 860, 1141, 1218
TseFI GTSAC 2 cut(s) 614, 1139
TseI GCWGC 2 cut(s) 521, 1199
Tsp45I GTSAC 2 cut(s) 614, 1139
TspDTI ATGAA 6 cut(s) 58, 221, 624, 636, 808, 1062
TspRI CASTG 3 cut(s) 860, 1141, 1218
VneI GTGCAC 1 cut(s) 1183
VpaK11BI GGWCC 2 cut(s) 306, 497
XapI RAATTY 4 cut(s) 55, 216, 922, 997
XbaI TCTAGA 1 cut(s) 1148
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 3 cut(s) 788, 929, 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.